AR125392A2 - Métodos y composiciones para la expresión génica en plantas - Google Patents

Métodos y composiciones para la expresión génica en plantas

Info

Publication number
AR125392A2
AR125392A2 ARP220101020A ARP220101020A AR125392A2 AR 125392 A2 AR125392 A2 AR 125392A2 AR P220101020 A ARP220101020 A AR P220101020A AR P220101020 A ARP220101020 A AR P220101020A AR 125392 A2 AR125392 A2 AR 125392A2
Authority
AR
Argentina
Prior art keywords
transit peptide
sequence encoding
sequence
recombinant dna
seq
Prior art date
Application number
ARP220101020A
Other languages
English (en)
Inventor
Clayton T Larue
Joel E Ream
Aabid Shariff
Yuanji Zhang
Xuefeng Zhou
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR125392A2 publication Critical patent/AR125392A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/07—Fusion polypeptide containing a localisation/targetting motif containing a mitochondrial localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Abstract

La invención proporciona moléculas de ADN recombinante útiles para proporcionar la expresión eficaz de proteínas en plantas transgénicas, así como composiciones y métodos para utilizar las moléculas de ADN recombinante. En modalidades particulares, la invención proporciona moléculas de ADN recombinante y construcciones que comprenden secuencias que codifican péptidos de tránsito y secuencias unidas operativamente que confieren tolerancia a herbicidas. Reivindicación 1: Una molécula de ADN recombinante que comprende una secuencia de ADN que codifica un péptido de tránsito unida operativamente a una secuencia de ADN que codifica una proteína con tolerancia a herbicidas heteróloga, caracterizada porque el péptido de tránsito comprende una secuencia de aminoácidos que comprende al menos 97 por ciento de identidad con la secuencia de SEQ ID Nº 241. Reivindicación 11: Una proteína recombinante, caracterizada porque comprende lo siguiente en unión operativa: a) un péptido de tránsito que comprende una secuencia de aminoácidos que comprende al menos 95 por ciento de identidad con la secuencia de SEQ ID Nº 241; y b) una proteína con tolerancia a herbicidas heteróloga. Reivindicación 27: Una molécula de ADN recombinante que comprende una secuencia de ADN que codifica un péptido de tránsito unida operativamente a una secuencia de ADN que codifica una proteína con tolerancia a herbicidas heteróloga, caracterizada porque el péptido de tránsito comprende una secuencia de aminoácidos que comprende al menos 95 por ciento de identidad con la secuencia de SEQ ID Nº 241. Reivindicación 33: Una molécula de ADN recombinante aislada que comprende una secuencia de ADN que codifica un péptido de tránsito unida operativamente a una secuencia de ADN que codifica una proteína con tolerancia a herbicidas heteróloga, caracterizada porque el péptido de tránsito comprende una secuencia de aminoácidos que comprende al menos 97 por ciento de identidad con la secuencia de SEQ ID Nº 241. Reivindicación 39: Una molécula de ADN recombinante aislada que comprende una secuencia de ADN que codifica un péptido de tránsito unida operativamente a una secuencia de ADN que codifica una proteína con tolerancia a herbicidas heteróloga, caracterizada porque el péptido de tránsito comprende una secuencia de aminoácidos que comprende al menos 95 por ciento de identidad con la secuencia de SEQ ID Nº 241.
ARP220101020A 2016-07-29 2022-04-20 Métodos y composiciones para la expresión génica en plantas AR125392A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201662368840P 2016-07-29 2016-07-29

Publications (1)

Publication Number Publication Date
AR125392A2 true AR125392A2 (es) 2023-07-12

Family

ID=61016595

Family Applications (4)

Application Number Title Priority Date Filing Date
ARP170102148A AR109196A1 (es) 2016-07-29 2017-07-28 Métodos y composiciones para la expresión génica en plantas
ARP220101022A AR125394A2 (es) 2016-07-29 2022-04-20 Métodos y composiciones para la expresión génica en plantas
ARP220101020A AR125392A2 (es) 2016-07-29 2022-04-20 Métodos y composiciones para la expresión génica en plantas
ARP220101021A AR125393A2 (es) 2016-07-29 2022-04-20 Métodos y composiciones para la expresión génica en plantas

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ARP170102148A AR109196A1 (es) 2016-07-29 2017-07-28 Métodos y composiciones para la expresión génica en plantas
ARP220101022A AR125394A2 (es) 2016-07-29 2022-04-20 Métodos y composiciones para la expresión génica en plantas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP220101021A AR125393A2 (es) 2016-07-29 2022-04-20 Métodos y composiciones para la expresión génica en plantas

Country Status (20)

Country Link
US (3) US10745712B2 (es)
EP (2) EP3797583A3 (es)
JP (1) JP7118047B2 (es)
KR (1) KR102587506B1 (es)
CN (1) CN109475099A (es)
AR (4) AR109196A1 (es)
AU (2) AU2017301813B2 (es)
BR (1) BR112019000923A2 (es)
CA (1) CA2974824A1 (es)
CL (2) CL2019000220A1 (es)
CO (1) CO2019001678A2 (es)
EC (1) ECSP19013893A (es)
MX (3) MX2019001215A (es)
PE (2) PE20240136A1 (es)
PH (1) PH12019500209A1 (es)
RU (1) RU2763534C2 (es)
UA (1) UA127343C2 (es)
UY (1) UY37347A (es)
WO (1) WO2018022777A1 (es)
ZA (1) ZA201900225B (es)

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Also Published As

Publication number Publication date
EP3797583A3 (en) 2021-06-23
AU2023229539A1 (en) 2023-10-05
CO2019001678A2 (es) 2019-03-08
US20230340519A1 (en) 2023-10-26
CA2974824A1 (en) 2018-01-29
AU2017301813B2 (en) 2023-06-15
EP3490366A1 (en) 2019-06-05
KR102587506B1 (ko) 2023-10-11
JP2019523004A (ja) 2019-08-22
UY37347A (es) 2018-02-28
RU2019103990A (ru) 2020-08-28
RU2019103990A3 (es) 2020-12-30
MX2020013900A (es) 2021-03-09
ZA201900225B (en) 2019-10-30
UA127343C2 (uk) 2023-07-26
MX2019001215A (es) 2019-06-03
AR125394A2 (es) 2023-07-12
PE20190838A1 (es) 2019-06-17
PE20240136A1 (es) 2024-01-30
RU2763534C2 (ru) 2021-12-30
EP3797583A2 (en) 2021-03-31
CN109475099A (zh) 2019-03-15
US10745712B2 (en) 2020-08-18
AR109196A1 (es) 2018-11-07
JP7118047B2 (ja) 2022-08-15
US11629358B2 (en) 2023-04-18
MX2021010925A (es) 2021-10-01
CL2020002234A1 (es) 2021-02-05
AU2017301813A1 (en) 2019-02-07
CL2019000220A1 (es) 2019-04-12
BR112019000923A2 (pt) 2019-04-30
WO2018022777A1 (en) 2018-02-01
US20210002663A1 (en) 2021-01-07
PH12019500209A1 (en) 2019-12-02
EP3490366A4 (en) 2020-04-22
US20180044690A1 (en) 2018-02-15
AR125393A2 (es) 2023-07-12
ECSP19013893A (es) 2019-03-29
KR20190033548A (ko) 2019-03-29

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