EA202091496A1 - Вариант инсектицидного гена axmi115 и способы его применения - Google Patents
Вариант инсектицидного гена axmi115 и способы его примененияInfo
- Publication number
- EA202091496A1 EA202091496A1 EA202091496A EA202091496A EA202091496A1 EA 202091496 A1 EA202091496 A1 EA 202091496A1 EA 202091496 A EA202091496 A EA 202091496A EA 202091496 A EA202091496 A EA 202091496A EA 202091496 A1 EA202091496 A1 EA 202091496A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- seq
- plants
- bacteria
- methods
- seeds
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 239000002917 insecticide Substances 0.000 title 1
- 241000894006 Bacteria Species 0.000 abstract 3
- 102000037865 fusion proteins Human genes 0.000 abstract 3
- 108020001507 fusion proteins Proteins 0.000 abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 210000004900 c-terminal fragment Anatomy 0.000 abstract 1
- 210000004898 n-terminal fragment Anatomy 0.000 abstract 1
- 230000000361 pesticidal effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Manufacture And Refinement Of Metals (AREA)
Abstract
Изобретение предлагает композиции и способы, придающие пестицидную активность бактериям, растениям, растительным клеткам, тканям и семенам. Последовательности, кодирующие токсины, можно использовать в составе конструкций ДНК или экспрессионных кассет для экспрессии в растениях и бактериях. Композиции также включают в себя трансформированные бактерии, растения, растительные клетки, ткани и семена. В частности, изобретение предлагает полинуклеотидные последовательности и кодируемые ими токсичные белки. Изобретение также предлагает антитела, способные специфически связываться с указанными аминокислотными последовательностями. В частности, изобретение охватывает полинуклеотидные последовательности, кодирующие гибридные белки, а также их биологически активные варианты и фрагменты, где гибридный белок содержит C-концевой фрагмент SEQ ID NO:43. Гибридный белок также может содержать N-концевой фрагмент SEQ ID NO:45. Изобретение также включает в себя нуклеотидную последовательность SEQ ID NO:47 и 1-14 или нуклеотидную последовательность, кодирующую аминокислотную последовательность, описанную в SEQ ID NO:48 и 15-31, в том числе ее биологически активные варианты и фрагменты.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161471848P | 2011-04-05 | 2011-04-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA202091496A1 true EA202091496A1 (ru) | 2020-09-30 |
Family
ID=45974517
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA202091496A EA202091496A1 (ru) | 2011-04-05 | 2012-04-04 | Вариант инсектицидного гена axmi115 и способы его применения |
| EA201391444A EA036053B1 (ru) | 2011-04-05 | 2012-04-04 | Вариант инсектицидного гена axmi115 и способы его применения |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201391444A EA036053B1 (ru) | 2011-04-05 | 2012-04-04 | Вариант инсектицидного гена axmi115 и способы его применения |
Country Status (18)
| Country | Link |
|---|---|
| US (4) | US20130097728A1 (ru) |
| EP (3) | EP3498849B1 (ru) |
| CN (2) | CN110055264A (ru) |
| AP (1) | AP2013007163A0 (ru) |
| AR (1) | AR085939A1 (ru) |
| AU (3) | AU2012240347A1 (ru) |
| BR (1) | BR112013025665B8 (ru) |
| CA (1) | CA2832087C (ru) |
| EA (2) | EA202091496A1 (ru) |
| ES (2) | ES2901155T3 (ru) |
| HU (1) | HUE045066T2 (ru) |
| MX (3) | MX367427B (ru) |
| PL (1) | PL2694659T3 (ru) |
| RS (1) | RS59239B1 (ru) |
| UA (3) | UA121184C2 (ru) |
| UY (1) | UY34005A (ru) |
| WO (1) | WO2012138703A1 (ru) |
| ZA (1) | ZA201307363B (ru) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2811698C (en) * | 2010-09-22 | 2020-02-18 | Bayer Intellectual Property Gmbh | Use of active ingredients for controlling nematodes in nematode-resistant crops |
| WO2013015993A1 (en) * | 2011-07-28 | 2013-01-31 | Syngenta Participations Ag | Methods and compositions for controlling nematode pests |
| BR112015023709A2 (pt) | 2013-03-15 | 2017-07-18 | Pioneer Hi Bred Int | polipeptídeo phi-4, polinucleotídeo, composição, método para inibir o crescimento, método para controlar uma população, planta, semente, cassete de expressão, método para expressar em uma planta um polinucleotídeo, método para proteger uma planta, proteína de fusão |
| EP3185895A4 (en) | 2014-08-29 | 2018-01-10 | Syngenta Participations AG | Modified vip3 polypeptides |
| US10155960B2 (en) * | 2015-08-27 | 2018-12-18 | Monsanto Technology Llc | Insect inhibitory proteins |
| BR112021002792A2 (pt) * | 2018-08-13 | 2021-06-29 | Pioneer Hi-Bred International, Inc. | novos receptores de toxinas inseticidas e métodos de uso |
| CA3115551A1 (en) * | 2019-01-22 | 2020-07-30 | Monsanto Technology Llc | Novel insect inhibitory proteins |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1506602A (en) | 1922-05-17 | 1924-08-26 | Nichols Henry | Vehicle wheel |
| US4196265A (en) | 1977-06-15 | 1980-04-01 | The Wistar Institute | Method of producing antibodies |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| DE3685968T2 (de) | 1985-01-22 | 1993-07-01 | Mycogen Corp | Zellulare einkapselung biologischer pestizide. |
| US5039523A (en) | 1988-10-27 | 1991-08-13 | Mycogen Corporation | Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin |
| AU642889B2 (en) | 1989-07-11 | 1993-11-04 | Biotechnology Research And Development Corporation | Aerosol beam microinjector |
| US5240842A (en) | 1989-07-11 | 1993-08-31 | Biotechnology Research And Development Corporation | Aerosol beam microinjector |
| CA2051562C (en) | 1990-10-12 | 2003-12-02 | Jewel M. Payne | Bacillus thuringiensis isolates active against dipteran pests |
| TW261517B (ru) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US5743477A (en) | 1992-08-27 | 1998-04-28 | Dowelanco | Insecticidal proteins and method for plant protection |
| US5849870A (en) * | 1993-03-25 | 1998-12-15 | Novartis Finance Corporation | Pesticidal proteins and strains |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6570005B1 (en) | 1996-07-01 | 2003-05-27 | Mycogen Corporation | Toxins active against pests |
| US7129212B2 (en) | 1996-10-30 | 2006-10-31 | Mycogen Corporation | Polynucleotides, pesticidal proteins, and novel methods of using them |
| EP0972062A2 (en) | 1997-04-03 | 2000-01-19 | Novartis AG | Plant pest control |
| US6468523B1 (en) | 1998-11-02 | 2002-10-22 | Monsanto Technology Llc | Polypeptide compositions toxic to diabrotic insects, and methods of use |
| US6938976B2 (en) | 1999-06-16 | 2005-09-06 | Eastman Kodak Company | Printer and method therefor adapted to sense data uniquely associated with a consumable loaded into the printer |
| WO2001038514A2 (en) | 1999-11-29 | 2001-05-31 | Midwest Oilseeds, Inc. | Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams |
| AU2001287862B2 (en) | 2000-09-29 | 2006-12-14 | Syngenta Limited | Herbicide resistant plants |
| AR035799A1 (es) * | 2001-03-30 | 2004-07-14 | Syngenta Participations Ag | Toxinas insecticidas aisladas de bacillus thuringiensis y sus usos. |
| JP2006500908A (ja) | 2002-03-06 | 2006-01-12 | シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト | 新規なＶｉｐ３トキシン及び使用方法 |
| WO2003080656A1 (en) * | 2002-03-22 | 2003-10-02 | Bayer Bioscience N.V. | Novel bacillus thuringiensis insecticidal proteins |
| EP3800198A1 (en) * | 2006-06-15 | 2021-04-07 | Basf Agricultural Solutions Seed Us Llc | A family of pesticidal proteins and methods for their use |
| US7973214B2 (en) | 2006-09-25 | 2011-07-05 | Ut-Battelle, Llc | Designer organisms for photosynthetic production of ethanol from carbon dioxide and water |
| CN101821744B (zh) | 2007-10-09 | 2013-05-22 | 阿森尼克斯公司 | 合成基因设计的计算方法 |
| CN110734919A (zh) * | 2008-07-02 | 2020-01-31 | 阿森尼克斯公司 | Axmi-il5、axmi-113、axmi-005、axmi-163和axmi-184∶vip3a杀虫蛋白及其使用方法 |
| AU2010278843B2 (en) * | 2009-07-31 | 2016-05-19 | BASF Agricultural Solutions Seed US LLC | Axmi-192 family of pesticidal genes and methods for their use |
-
2012
- 2012-04-04 AR ARP120101158A patent/AR085939A1/es active IP Right Grant
- 2012-04-04 WO PCT/US2012/032086 patent/WO2012138703A1/en active Application Filing
- 2012-04-04 ES ES19153784T patent/ES2901155T3/es active Active
- 2012-04-04 UA UAA201905281A patent/UA121184C2/uk unknown
- 2012-04-04 CA CA2832087A patent/CA2832087C/en active Active
- 2012-04-04 US US13/439,269 patent/US20130097728A1/en not_active Abandoned
- 2012-04-04 CN CN201910218413.XA patent/CN110055264A/zh active Pending
- 2012-04-04 PL PL12714905T patent/PL2694659T3/pl unknown
- 2012-04-04 BR BR112013025665A patent/BR112013025665B8/pt active IP Right Grant
- 2012-04-04 UA UAA201311758A patent/UA120584C2/uk unknown
- 2012-04-04 HU HUE12714905A patent/HUE045066T2/hu unknown
- 2012-04-04 RS RSP20191164 patent/RS59239B1/sr unknown
- 2012-04-04 EP EP19153784.4A patent/EP3498849B1/en active Active
- 2012-04-04 AU AU2012240347A patent/AU2012240347A1/en not_active Abandoned
- 2012-04-04 CN CN201280027517.9A patent/CN103635579B/zh active Active
- 2012-04-04 EA EA202091496A patent/EA202091496A1/ru unknown
- 2012-04-04 AP AP2013007163A patent/AP2013007163A0/xx unknown
- 2012-04-04 UA UAA201911882A patent/UA122856C2/uk unknown
- 2012-04-04 MX MX2016015033A patent/MX367427B/es unknown
- 2012-04-04 MX MX2013011580A patent/MX344229B/es active IP Right Grant
- 2012-04-04 ES ES12714905T patent/ES2743322T3/es active Active
- 2012-04-04 EA EA201391444A patent/EA036053B1/ru not_active IP Right Cessation
- 2012-04-04 EP EP12714905.2A patent/EP2694659B1/en active Active
- 2012-04-04 EP EP21187009.2A patent/EP3971297A1/en active Pending
- 2012-04-09 UY UY0001034005A patent/UY34005A/es active IP Right Grant
-
2013
- 2013-10-02 ZA ZA2013/07363A patent/ZA201307363B/en unknown
- 2013-10-04 MX MX2019009896A patent/MX2019009896A/es unknown
-
2015
- 2015-09-11 US US14/851,442 patent/US9752158B2/en active Active
- 2015-09-11 US US14/851,555 patent/US9982274B2/en active Active
-
2017
- 2017-04-26 AU AU2017202747A patent/AU2017202747A1/en not_active Abandoned
-
2018
- 2018-04-24 US US15/960,817 patent/US10647996B2/en active Active
-
2019
- 2019-07-31 AU AU2019210561A patent/AU2019210561B2/en active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EA201170980A1 (ru) | Пестицидные белки и способы их применения | |
| EA201200199A1 (ru) | Семейство axmi-192 пестицидных генов и способы их применения | |
| EA201071323A1 (ru) | Axmi-115, axmi-113, axmi-005, axmi-163, axmi-184 инсектицидные белки и способы их применения | |
| EA201490379A1 (ru) | Варианты белков axmi205 и способы их применения | |
| EA201070371A1 (ru) | Axmi-066 и axmi-076: дельта-эндотоксиновые белки и способы их применения | |
| EA201490374A1 (ru) | Ген пестицида axmi279 и способы его применения | |
| EA202091496A1 (ru) | Вариант инсектицидного гена axmi115 и способы его применения | |
| EA201170717A1 (ru) | Дельта-эндотоксиновый ген axmi-150 и способы его применения | |
| BR112012020921A2 (pt) | genes de delta-endotoxina axmi218, axmi219, axmi220, axmi226, axmi227, axmi228, axmi229, axmi230 e axmi231 e métodos para sua utilização | |
| EA201400248A1 (ru) | Гены токсинов и способы их применения | |
| WO2010141141A3 (en) | Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: insecticidal proteins from bacillus thuringiensis and methods for their use | |
| AR061467A1 (es) | Axmi-031 axmi-039 axmi-040 y axmi-049 una familia de nuevos genes de delta-endotoxinas y metodos para usarlos | |
| AR080200A1 (es) | Genes delta-endotoxinicos axmi221z, axmi222z, axmi223z, axmi224z, y axmi225z y metodos para su uso | |
| MX2014000993A (es) | Gen de la toxina axmi270 y sus metodos de uso. | |
| EA201170961A1 (ru) | Новый ген bacillus thuringiensis с активностью против lepidoptera | |
| EA201170685A1 (ru) | Гены, кодирущие токсины нематод | |
| MX362999B (es) | Gen de la delta-endotoxina axmi345 y sus metodos de uso. | |
| EA201691192A1 (ru) | Гены токсинов axmi477, axmi482, axmi486 и axmi525 и способы их применения | |
| BR112014022034A2 (pt) | gene da toxina axmi335 de bacillus thuringiensis e métodos para a sua utilização | |
| EA201071400A1 (ru) | Новый ген bacillus thuringiensis с активностью против lepidopteran (чешуекрылых) | |
| UA117731C2 (uk) | Токсин axmi277 проти нематод та його застосування | |
| EA201991665A3 (ru) | Гены токсинов axmi477, axmi482, axmi486 и axmi525 и способы их применения | |
| BR112013033592A8 (pt) | Toxina nematoide axmi277 e métodos para a sua utilização |