BR112013033592A8 - Toxina nematoide axmi277 e métodos para a sua utilização - Google Patents

Toxina nematoide axmi277 e métodos para a sua utilização

Info

Publication number
BR112013033592A8
BR112013033592A8 BR112013033592A BR112013033592A BR112013033592A8 BR 112013033592 A8 BR112013033592 A8 BR 112013033592A8 BR 112013033592 A BR112013033592 A BR 112013033592A BR 112013033592 A BR112013033592 A BR 112013033592A BR 112013033592 A8 BR112013033592 A8 BR 112013033592A8
Authority
BR
Brazil
Prior art keywords
toxin
methods
plants
compositions
amino acid
Prior art date
Application number
BR112013033592A
Other languages
English (en)
Other versions
BR112013033592A2 (pt
Inventor
Poutre Candace
W Kahn Theodore
Anthony Alissa
Original Assignee
Athenix Corp
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Athenix Corp, Basf Se filed Critical Athenix Corp
Priority claimed from PCT/US2012/044751 external-priority patent/WO2014003769A1/en
Publication of BR112013033592A2 publication Critical patent/BR112013033592A2/pt
Publication of BR112013033592A8 publication Critical patent/BR112013033592A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

TOXINA NEMATOIDE AXMI277 E MÉTODOS PARA A SUA UTILIZAÇÃO. São fornecidas composições e métodos para conferir atividade pesticida a bactérias, plantas, células vegetais, tecidos e sementes. São fornecidas composições compreendendo uma sequência codificadora para um polipeptídeo de toxina. As sequências codificadoras podem ser usadas em construções de DNA ou em cassetes de expressão para a transformação e expressão em plantas e bactérias. As composições também compreendem bactérias transformadas, plantas, células vegetais, tecidos e sementes. Em particular, são fornecidas moléculas de ácido nucleico de toxina isoladas. Adicionalmente, são abrangidas as sequências de aminoácidos correspondentes aos polinucleótidos, e anticorpos que se ligam especificamente a estas sequências de aminoácidos. Em particular, a presente invenção fornece moléculas de ácidos nucleicos isoladas compreendendo sequências nucleotídicas que codificam a sequência de aminoácidos mostrada na SEQ ID NO:2 ou 3, ou a sequência de nucleótidos descrita em SEQ ID NO:1, bem como variantes e fragmentos das mesmas.
BR112013033592A 2011-06-30 2012-06-29 Toxina nematoide axmi277 e métodos para a sua utilização BR112013033592A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161503132P 2011-06-30 2011-06-30
US61/503,132 2011-06-30
PCT/US2012/044751 WO2014003769A1 (en) 2012-06-29 2012-06-29 Axmi277 nematode toxin and methods for its use

Publications (2)

Publication Number Publication Date
BR112013033592A2 BR112013033592A2 (pt) 2021-05-04
BR112013033592A8 true BR112013033592A8 (pt) 2022-07-05

Family

ID=46545547

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013033592A BR112013033592A8 (pt) 2011-06-30 2012-06-29 Toxina nematoide axmi277 e métodos para a sua utilização

Country Status (5)

Country Link
US (1) US20140380521A1 (pt)
EP (1) EP2726617B8 (pt)
BR (1) BR112013033592A8 (pt)
RU (1) RU2706488C2 (pt)
ZA (1) ZA201400199B (pt)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589382A (en) * 1990-07-27 1996-12-31 Mycogen Corporation Bacillus thuringiensis genes encoding nematode-active toxins
RU2286385C2 (ru) * 2004-11-23 2006-10-27 Центр "Биоинженерия" Ран РЕКОМБИНАНТНАЯ ПОЛИНУКЛЕОТИДНАЯ ПОСЛЕДОВАТЕЛЬНОСТЬ, ХАРАКТЕРИЗУЮЩАЯ УНИКАЛЬНЫЙ ТРАНСФОРМАЦИОННЫЙ АКТ МЕЖДУ ГЕНЕТИЧЕСКОЙ КОНСТРУКЦИЕЙ, ВКЛЮЧАЮЩЕЙ ГЕН cryIIIa, И ГЕНОМНОЙ ДНК КАРТОФЕЛЯ СОРТА ЕЛИЗАВЕТА, ЕЕ ПРИМЕНЕНИЕ И СОДЕРЖАЩИЕ ЭТУ ПОСЛЕДОВАТЕЛЬНОСТЬ КЛЕТКА, ТРАНСГЕННОЕ РАСТЕНИЕ И ЕГО ПОТОМСТВО
AR061467A1 (es) * 2006-06-14 2008-08-27 Athenix Corp Axmi-031 axmi-039 axmi-040 y axmi-049 una familia de nuevos genes de delta-endotoxinas y metodos para usarlos
BRPI0917159A2 (pt) * 2008-08-25 2015-07-28 Down Agrosciences Llc Proteínas cry14 modificadas do bacillus trhuringiensis para o controle de nematódeos
EP2666866A3 (en) * 2009-03-06 2014-03-05 Athenix Corporation Methods and compositions for controlling plant pests

Also Published As

Publication number Publication date
BR112013033592A2 (pt) 2021-05-04
EP2726617B1 (en) 2019-01-16
EP2726617A1 (en) 2014-05-07
US20140380521A1 (en) 2014-12-25
RU2706488C2 (ru) 2019-11-19
EP2726617B8 (en) 2019-04-17
RU2014102759A (ru) 2015-08-10
ZA201400199B (en) 2016-08-31

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]
B25A Requested transfer of rights approved

Owner name: BASF SE (DE)

B25A Requested transfer of rights approved

Owner name: BASF AGRICULTURAL SOLUTIONS SEED US LLC (US)