EA201200199A1 - Семейство axmi-192 пестицидных генов и способы их применения - Google Patents

Семейство axmi-192 пестицидных генов и способы их применения

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Publication number
EA201200199A1
EA201200199A1 EA201200199A EA201200199A EA201200199A1 EA 201200199 A1 EA201200199 A1 EA 201200199A1 EA 201200199 A EA201200199 A EA 201200199A EA 201200199 A EA201200199 A EA 201200199A EA 201200199 A1 EA201200199 A1 EA 201200199A1
Authority
EA
Eurasian Patent Office
Prior art keywords
plants
compositions
amino acid
bacteria
methods
Prior art date
Application number
EA201200199A
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English (en)
Other versions
EA035563B1 (ru
Inventor
Кимберли С. Сэмпсон
Дэниел Джон Томсо
Жун Го
Original Assignee
Атеникс Корп.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Атеникс Корп. filed Critical Атеникс Корп.
Publication of EA201200199A1 publication Critical patent/EA201200199A1/ru
Publication of EA035563B1 publication Critical patent/EA035563B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2/00—Peptides of undefined number of amino acids; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pest Control & Pesticides (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Предложены композиции и способы для придания пестицидной активности бактериям, растениям, растительным клеткам, тканям и семенам. Предложены композиции, включающие кодирующую последовательность для полипептида токсина. Кодирующие последовательности могут быть использованы в конструктах ДНК или кассетах экспрессии для трансформации и экспрессии в растениях и бактериях. Композиции также включают трансформированные бактерии, растения, растительные клетки, ткани и семена. В частности, предложены выделенные молекулы нуклеиновой кислоты токсина. Дополнительно охвачены аминокислотные последовательности, соответствующие этим полинуклеотидам, и антитела, специфически связывающиеся с этими аминокислотными последовательностями. В частности, данное изобретение обеспечивает выделенные молекулы нуклеиновой кислоты, включающие нуклеотидные последовательности, кодирующие аминокислотную последовательность, показанную в SEQ ID NO: 28-62, или нуклеотидную последовательность, приведенную в SEQ ID NO: 1-27, а также их варианты и фрагменты.
EA201200199A 2009-07-31 2010-07-30 Молекула рекомбинантной нуклеиновой кислоты, кодирующей полипептид с пестицидной активностью против чешуекрылых, жесткокрылых или нематодных вредителей, ее получение и применение EA035563B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23065909P 2009-07-31 2009-07-31
PCT/US2010/043871 WO2011014749A1 (en) 2009-07-31 2010-07-30 Axmi-192 family of pesticidal genes and methods for their use

Publications (2)

Publication Number Publication Date
EA201200199A1 true EA201200199A1 (ru) 2012-06-29
EA035563B1 EA035563B1 (ru) 2020-07-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201200199A EA035563B1 (ru) 2009-07-31 2010-07-30 Молекула рекомбинантной нуклеиновой кислоты, кодирующей полипептид с пестицидной активностью против чешуекрылых, жесткокрылых или нематодных вредителей, ее получение и применение

Country Status (11)

Country Link
US (2) US8461415B2 (ru)
EP (1) EP2459587B1 (ru)
CN (2) CN102656185B (ru)
AR (1) AR077344A1 (ru)
AU (2) AU2010278843B2 (ru)
BR (1) BR112012002275B8 (ru)
CA (2) CA2769643C (ru)
EA (1) EA035563B1 (ru)
MX (2) MX2012001426A (ru)
WO (1) WO2011014749A1 (ru)
ZA (1) ZA201200723B (ru)

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Also Published As

Publication number Publication date
EP2459587A1 (en) 2012-06-06
AU2016213694B2 (en) 2017-10-19
US20130303440A1 (en) 2013-11-14
BR112012002275A2 (pt) 2016-11-08
CA3062192A1 (en) 2011-02-03
CA2769643A1 (en) 2011-02-03
CN102656185B (zh) 2018-04-24
CN108484739A (zh) 2018-09-04
ZA201200723B (en) 2014-03-26
AU2010278843A1 (en) 2012-02-16
CA3062192C (en) 2022-05-17
MX354219B (es) 2018-02-19
CN108484739B (zh) 2022-08-30
BR112012002275B8 (pt) 2022-07-05
CA2769643C (en) 2020-01-07
WO2011014749A1 (en) 2011-02-03
US20110030096A1 (en) 2011-02-03
EA035563B1 (ru) 2020-07-08
EP2459587B1 (en) 2016-03-16
BR112012002275B1 (pt) 2022-02-01
MX2012001426A (es) 2012-03-26
AR077344A1 (es) 2011-08-17
AU2010278843B2 (en) 2016-05-19
CN102656185A (zh) 2012-09-05
AU2016213694A1 (en) 2016-09-01
US8461415B2 (en) 2013-06-11

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