CN108484739A - 杀虫基因的axmi-192家族和使用它们的方法 - Google Patents

杀虫基因的axmi-192家族和使用它们的方法 Download PDF

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CN108484739A
CN108484739A CN201810244642.4A CN201810244642A CN108484739A CN 108484739 A CN108484739 A CN 108484739A CN 201810244642 A CN201810244642 A CN 201810244642A CN 108484739 A CN108484739 A CN 108484739A
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K·S·桑普森
D·J·汤姆索
R·郭
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Abstract

提供了对细菌、植物、植物细胞、组织和种子赋予杀虫活性的组合物和方法。提供了包含毒素多肽编码序列的组合物。所述编码序列可以在用于植物和细菌中转化和表达的DNA构建体或表达盒中使用。组合物也包含转化的细菌、植物、植物细胞、组织和种子。特别地，提供了分离的毒素核酸分子。另外，本发明包含与所述多核苷酸对应的氨基酸序列和与那些氨基酸序列特异性结合的抗体。特别地，本发明提供了分离的核酸分子以及其变体和片段，所述分离的核酸分子包含编码SEQ ID NO:28‑62中所示氨基酸序列的核苷酸序列或SEQ ID NO:1‑27中所示的核苷酸序列。

Description

杀虫基因的AXMI-192家族和使用它们的方法
本申请是中国专利申请201080043772.3的分案申请，原申请的申请日是2010年7月30日，发明名称是“杀虫基因的AXMI-192家族和使用它们的方法”。
相关申请的交互参考
本申请要求2009年7月31日提交的美国临时专利申请系列号61/230,659的权益，所述专利申请的内容均通过引用方式完整并入本文作为参考。
参考电子提交的序列表
通过EFS-Web将序列表的正式文本电子提交为2010年7月27日创建的具有文件名“APA067US01_SEQLIST.txt”的ASCII格式序列表，并且其具有273千字节的大小且与本说明书同时提交。在该ASCII格式文件中包含的序列表是本说明书的一部分并将其通过引用方式完整并入本文作为参考。
发明领域
本发明涉及分子生物学领域。提供了编码杀虫蛋白的新基因。这些蛋白质和编码它们的核酸序列在制备杀虫制剂中并且在产生转基因抗害虫植物中有用。
发明背景
苏云金芽孢杆菌(Bacillus thuringiensis)是形成孢子的革兰氏阳性土壤细菌，其特征在于它产生晶状包涵体的能力，其中所述的晶状包涵体特异性地对某些目和种的昆虫有毒，但是对植物和其他非靶向的生物无害。出于这个原因，包括苏云金芽孢杆菌菌株或它们的杀昆虫性蛋白质的组合物可以作为环境可接受的杀虫剂用来控制农业害虫或多种人或动物疾病的昆虫媒介。
来自苏云金芽孢杆菌的晶体(Cry)蛋白(Δ-内毒素)具有主要针对鳞翅目的、双翅目的和鞘翅目的幼虫的强力杀昆虫活性。这些蛋白质也显示针对膜翅目(Hymenoptera)、同翅目(Homoptera)、虱目(Phthiraptera)、食毛目(Mallophaga)和蜱螨亚纲(Acari)害虫类以及其他无脊椎动物类如线虫类动物(Nemathelminthe)、扁形虫(Platyhelminthe)和Sarcomastigorphora的活性(Feitelson(1993)The Bacillus Thuringiensis familytree.在Advanced Engineered Pesticides中,Marcel Dekker,Inc.,New York,N.Y.)。这些蛋白质起初主要基于它们的杀昆虫活性分类为CryI至CryV。主要类型是鳞翅目特异的(I)、鳞翅目与双翅目特异的(II)、鞘翅目特异的(III)、双翅目特异的(IV)和线虫特异的(V)与(VI)。所述蛋白质进一步划分成亚家族；每个家族内部更高度相关的蛋白质被给予区分性字母如Cry1A、Cry1B、Cry1C等。在每个部分内部甚至更密切相关的蛋白质给予名称，如Cry1C1、Cry1C2等。
基于氨基酸序列同源性而非昆虫靶特异性，最近描述了Cry基因的一种新命名法(Crickmore等人,(1998)Microbiol.Mol.Biol.Rev.62:807-813)。在这种新分类中，每种毒素赋予一个独特名称，包含初阶(阿拉伯数字)、次阶(大写字母)、三阶(小写字母)和四阶(另一个阿拉伯数字)。在这个新分类法中，罗马数字已经更换为初阶中的阿拉伯数字。序列同一性小于45％的蛋白质具有不同的初阶，并且次阶和三阶的标准分别是78％和95％。
晶体蛋白在昆虫中肠中被摄取和溶解之前不显示杀昆虫活性。所摄取的前毒素(protoxin)由昆虫消化道中的蛋白酶水解成活性有毒分子。(和Whiteley(1989)Microbiol.Rev.53:242-255)。这种毒素与靶幼虫中肠中的顶部刷状缘受体结合并插入顶膜，产生离子通道或孔，从而导致幼虫死亡。
Δ-内毒素通常具有5个保守的序列结构域和3个保守的结构性结构域(见，例如,de Maagd等人,(2001)Trends Genetics 17:193-199)。第一个保守的结构性结构域由7个α螺旋组成并且参与膜插入和孔形成。结构域II由以希腊钥构型排列的3个β-片层组成，并且结构域III由“簿卷饼型”形式的2个反平行β-片层组成(de Maagd等人,2001，见上文)。结构域II和III参与受体识别和结合，并且因此被视为毒素特异性的决定因素。
除Δ-内毒素之外，还存在几种其他已知类型的杀虫蛋白毒素。VIP1/VIP2毒素(见，例如，美国专利号5,770,696)是通过下述机制对昆虫显示强烈活性的双元杀虫毒素，其中所述机制据信涉及受体介导的内吞作用，随后是细胞中毒，其类似于其他双元(“A/B”)毒素的作用模式。A/B毒素如VIP、C2、CDT、CST或炭疽芽孢杆菌(B.anthracis)水肿和致死毒素起初借助作为单体的“B”组分的受体介导的特异性结合与靶细胞相互作用。这些单体随后形成同七聚体。“B”七聚体-受体复合体随后作为停靠平台发挥作用，所述的停靠平台依次结合并允许酶促“A”组分借助受体介导的内吞作用转位至细胞溶胶中。一旦在细胞的细胞溶胶内部，“A”组分通过例如G-肌动蛋白的ADP-核糖基化或增加环AMP(cAMP)胞内水平抑制正常的细胞功能。见Barth等人,(2004)Microbiol Mol Biol Rev68:373—402。
密集使用基于苏云金芽孢杆菌的杀虫剂已经在小菜蛾(Plutella xylostella)田间种群中导致抗性(Ferré和Van Rie(2002)Annu.Rev.Entomol.47:501-533)。最常见的抗性机制是减少毒素与其特异性中肠受体的结合。这也可以赋予针对相同受体的其他毒素的交叉抗性(Ferré和Van Rie(2002))。
发明概述
提供了用于赋予害虫抗性至细菌、植物、植物细胞、组织和种子的组合物和方法。组合物包括编码毒素多肽的序列的核酸分子、包含这些核酸分子的载体和包含所述载体的宿主细胞。组合物也包括该毒素的多肽序列和针对那些多肽的抗体。所述核苷酸序列可以在用于生物(包括微生物和植物)中转化和表达的DNA构建体或表达盒中使用。所述核苷酸或氨基酸序列可以是已经设计用于在生物中表达的合成序列，所述的生物包括但不限于微生物和植物。组合物也包含转化的细菌、植物、植物细胞、组织和种子。
特别地，提供了与毒素核酸序列对应的分离的或者重组的核酸分子。额外地，包括与所述多核苷酸相对应的氨基酸序列。特别地，本发明提供了分离的核酸分子以及其变体和片段，所述的分离核酸分子包含编码任一SEQ ID NO:28-62所示氨基酸序列的核苷酸序列或任一SEQ ID NO:1-27所示的核苷酸序列。也包括与本发明核苷酸序列互补或与本发明序列杂交的核苷酸序列。
本发明的组合物和方法用于产生具有杀虫剂抗性的生物，特别地是细菌和植物。这些生物和从它们衍生的组合物对农业目的而言是希望的。本发明的组合物也用于产生具有杀虫活性的改变或改良的毒素蛋白，或用于检测产品或生物中毒素蛋白或核酸的存在。
发明详述
本发明涉及用于调节生物中、特别是植物或植物细胞中害虫抗性或耐受性的组合物和方法。“抗性”意指摄取或者另外地接触本发明的多肽后杀死害虫(例如昆虫)。“耐受性”意指削弱或者降低害虫的移动、进食、繁殖或者其他功能。所述方法涉及用编码本发明杀虫蛋白的核苷酸序列转化生物。特别地，本发明的核苷酸序列用于制备拥有杀虫活性的植物和微生物。因而，提供了转化的细菌、植物、植物细胞、植物组织和种子。组合物是细菌物种的杀虫核酸和蛋白质。所述序列用于构建随后转化至目的生物中的表达载体，作为探针用于分离其他同源的(或部分同源的)基因，和用于通过本领域已知方法(如结构域交换法(domain swapping)或DNA改组法)产生改变的杀虫蛋白。所述蛋白质用于控制或杀死鳞翅目的、鞘翅目的、双翅目的和线虫类的害虫种群并且用于产生具有杀虫活性的组合物。
“杀虫毒素”或“杀虫蛋白”意指针对一种或多种害虫具有毒害活性的毒素或者与该蛋白质具有同源性的蛋白质，其中所述的害虫包括但不限于鳞翅目(Lepidoptera)、双翅目(Diptera)和鞘翅目(Coleopter)或线虫纲(Nematoda)的成员。杀虫蛋白已经从生物，包括例如芽孢杆菌属物种(Bacillus sp.)、双酶梭菌(Clostridium bifermentans)和Paenibacillus popilliae分离。杀虫蛋白包括从本文所公开的全长核苷酸序列推导的氨基酸序列和因使用下游备选起始位点或因产生具有杀虫活性的更短蛋白质的加工作用而短于全长序列的氨基酸序列。加工可以在表达该蛋白质的生物中或在摄取该蛋白质后的害虫中发生。
在多个实施方案中，本文中所公开的序列与Δ-内毒素蛋白具有同源性。Δ-内毒素包括鉴定为cry1至cry53、cyt1和cyt2和Cyt样毒素的蛋白质。目前存在超过250个具有广泛类型特异性和毒性的Δ-内毒素已知种类。对于广泛列表，见Crickmore等人(1998),Microbiol.Mol.Biol.Rev.62:807-813，并且对于定期更新内容，见在www.biols.susx.ac.uk/Home/Neil_Crickmore/Bt/index处的Crickmore等人(2003)“Bacillus thuringiensis toxin nomenclature(苏云金芽孢杆菌毒素命名法)”。在一些实施方案中，本文中所公开的Δ-内毒素序列包括任一SEQ ID NO:1-27所述的核苷酸序列、任一SEQ ID NO:28-62所述的氨基酸序列、以及它们的变体和片段。
因此，本文提供了新分离的或重组的赋予杀虫活性的核苷酸序列。还提供了杀虫蛋白质的氨基酸序列。产生自这种基因的翻译的蛋白质允许细胞控制或者杀死摄取它的害虫。
分离的核酸分子及其变体和片段
本发明的一个方面涉及分离或重组的核酸分子，其包含编码杀虫蛋白和多肽或其生物学活性部分的核苷酸序列，以及这样的核酸分子，其足以用作杂交探针来鉴定编码具有序列同源性区域的蛋白质的核酸分子。如本文中所用，术语“核酸分子”意图包括DNA分子(例如，重组DNA、cDNA或基因组DNA)和RNA分子(例如，mRNA)和使用核苷酸类似物产生的DNA或RNA的类似物。核酸分子可以是单链的或双链的，但优选是双链DNA。
本文中所用的“分离的”核酸序列(或DNA)指已不再在其天然环境，例如在体外或者在重组的细菌或植物宿主细胞中的核酸序列(或DNA)。在一些实施方案中，“分离的”核酸不含在衍生该核酸的生物的基因组DNA中天然分布于该核酸侧翼的序列(即，位于该核酸的5'端和3'端处的序列)(优选地是蛋白质编码序列)。出于本发明的目的，用来指核酸分子时，“分离的”核酸分子排除分离的染色体。例如，在多个实施方案中，分离的编码杀虫蛋白质的核酸分子可以含有小于约5kb、4kb、3kb、2kb、1kb、0.5kb或0.1kb的下述核苷酸序列，所述的核苷酸序列在衍生该核酸分子的细胞的基因组DNA中天然分布于该核酸分子侧翼。基本上不含细胞物质的杀虫蛋白包括具有少于约30％、20％、10％或5％(以干重计)的非杀虫蛋白(本文中也称作“杂质蛋白”)的蛋白制备物。
编码本发明蛋白质的核苷酸序列包括SEQ ID NO:1-27中所述的序列及其变体、片段和互补物。“互补物”意指与给定核苷酸序列充分互补，从而它可以与给定核苷酸序列杂交以因而形成稳定双链体的核苷酸序列。由该核苷酸序列编码的毒素蛋白的对应氨基酸序列在SEQ ID NO:28-62中给出。
作为这些编码杀虫蛋白的核苷酸序列的片段的核酸分子也由本发明包括。“片段”意指编码杀虫蛋白的核苷酸序列的一部分。核苷酸序列的片段可以编码杀虫蛋白的生物学活性部分，或它可以是使用下文公开的方法时可以作为杂交探针或PCR引物使用的片段。取决于预期用途，作为编码杀虫蛋白的核苷酸序列的片段的核酸分子包含至少约50、100、200、300、400、500、600、700、800、900、1000、1100、1200、1300、1350、1400、1500、1600、1700、1800、1900、2000、2500、3000、3500、4000、4500、5000个连续核苷酸，或者直至本文中所公开的编码杀虫蛋白的全长核苷酸序列中存在的核苷酸数目。“连续的”核苷酸意指彼此紧密相邻的核苷酸残基。本发明核苷酸序列的片段将编码保留杀虫蛋白的生物学活性并且因而保留杀虫活性的蛋白质片段。“保留活性”意指该片段将具有该杀虫蛋白至少约30％、至少约50％、至少约70％、80％、90％、95％或更高的杀虫活性。在一个实施方案中，杀虫活性是杀鞘翅目的活性。在另一实施方案中，杀虫活性是杀鳞翅目的活性。在另一实施方案中，杀虫活性是杀线虫的活性。在另一实施方案中，杀虫活性是杀双翅目的活性。用于测量杀虫活性的方法是本领域熟知的。见，例如，Czapla和Lang(1990)J.Econ.Entomol.83:2480-2485；Andrews等人(1988)Biochem.J.252:199-206；Marrone等人(1985)J.of EconomicEntomology 78:290-293；和美国专利号5,743,477，所述全部文献均通过引用方式完整并入本文作为参考。
编码本发明杀虫蛋白的生物活性部分的核苷酸序列片段将编码至少约15、25、30、50、75、100、125、150、175、200、250、300、350、400、450、500、550、600、650、700、750、800、850、900、950、1000、1050、1100个连续氨基酸，或者直至本发明全长杀虫蛋白中存在的氨基酸总数。在一些实施方案中，该片段是相对于本发明的氨基酸序列具有至少约10、20、30、40、50、60、70、80、90、100、110、120、130、140、150、160、170、180、190、200、250、300、400、450、500、650、600个或者更多氨基酸的C末端截短。
本发明的优选杀虫蛋白由与SEQ ID NO:1-27的核苷酸序列充分同一的核苷酸序列编码。“充分同一的”意指使用标准参数，使用本文中所述的比对程序之一，与参考序列相比，具有至少约60％或65％序列同一性、约70％或75％序列同一性、约80％或85％序列同一性、约90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高序列同一性的氨基酸或核苷酸序列。本领域技术人员将会认识到通过考虑密码子简并性、氨基酸相似性、可读框定位等，可以适当调整这些值以确定两个核苷酸序列编码的蛋白质的相应同一性。
为确定两个氨基酸序列或两个核酸的同一性百分数，所述序列为了最佳比较目的进行比对。这两个序列之间的同一性百分数是所述序列共有的相同位置的数值的函数(即，同一性百分数＝相同位置数/总位置数(例如，重叠位置)x 100)。在另一个实施方案中，比较遍及参考序列的全部范围(即，遍及任一SEQ ID NO:1-61的全部范围)。可以使用与下文所述那些技术相似的技术，在容许或不容许空位的情况下确定两个序列之间的同一性百分数。在计算同一性百分数中，一般计数精确匹配。
使用数学算法，可以完成两个序列之间同一性百分数的确定。用于比较两个序列的数学算法的非限制性实例是Karlin和Altschul,(1990)Proc.Natl.Acad.Sci.USA 87:2264的算法，该算法如Karlin和Altschul,(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877中那样修改。该算法集成至Altschul等人,(1990)J.Mol.Biol.215:403的BLASTN和BLASTX程序中。BLAST核苷酸搜索可以在采用BLASTN程序，评分＝100、字长度＝12的情况下执行以获得与本发明的杀虫样核酸分子同源的核苷酸序列。可以用BLASTX程序、评分＝50、字长度＝3进行BLAST蛋白质搜索以获得同源于本发明杀虫蛋白质分子的氨基酸序列。为获得用于比较目的的空位比对，可以如Altschul等人,(1997)Nucleic Acids Res.25:3389中所述那样使用空位BLAST(在BLAST 2.0中)。备选地，PSI-Blast可以用来执行检测分子之间远缘关系的重复搜索。见上文Altschul等人,(1997)。当使用BLAST、空位BLAST和PSI-Blast程序时，可以使用相应程序(例如，BLASTX和BLASTN)的默认参数。比对也可以通过目测法人工地进行。
用来比较序列的数学算法的另一个非限制性实例是ClustalW算法(Higgins等人(1994)Nucleic acids Res.22:4673-4680)。ClustalW比较序列和比对氨基酸或DNA序列的完整性，并且因而可以提供关于完整氨基酸序列的序列保守性的数据。ClustalW算法在几个市售的DNA/氨基酸分析软件包中使用，如Vector NTI程序包的ALTGNX模块(InvitrogenCorporation,Carlsbad,CA)。在用ClustalW比对氨基酸序列后，可以评估氨基酸同一性百分数。用于分析ClustalW比对的软件程序的非限制实例是GENEDOCTM。GENEDOCTM(KarlNicholas)允许评估多种蛋白质之间的氨基酸(或DNA)相似性和同一性。另一个用于序列比较的非限制性数学算法的实例是Myers和Miller,(1988)CABIOS 4:11-17的算法。该算法集成至作为(从美国加利福尼亚州圣迭戈Accelrys,Inc.,9685Scranton Rd.可获得的)GCGWisconsin遗传学软件包版本10的一部分的ALIGN程序(版本2.0)。当使用ALIGN程序以比较氨基酸序列时，可以使用PAM120权重残基表、空位长度罚分12和空位罚分4。
除非另外说明，使用Needleman和Wunsch算法(1970)J.Mol.Biol.48(3):443-453的GAP第10版，使用以下参数：对于核苷酸序列的％同一性和％相似性，使用GAP权重50和长度权重3及nwsgapdna.cmp评分矩阵；对于氨基酸序列的％同一性或％相似性，使用GAP权重8和长度权重2及BLOSUM62评分程序，确定序列同一性或相似性。也可以使用等同程序。“等同程序”意指任何序列比较程序，其中对于所讨论的任意两个序列而言，与GAP第10版所产生的相应比对结果比较时，所述的序列比较程序产生具有相同核苷酸残基匹配和相同序列同一性百分数的比对结果。
本发明也包括变体核酸分子。编码杀虫蛋白的核苷酸序列的“变体”包括编码本文公开的杀虫蛋白、但是因为遗传密码简并性而保守地不同的那些序列，以及如上文所讨论的充分同一的那些序列。天然存在的等位基因变体可以使用熟知的分子生物学技术鉴定，如下文说明的聚合酶链反应(PCR)和杂交技术。变体核苷酸序列也包括合成方式衍生的核苷酸序列，所述核苷酸序列例如通过使用位点定向诱变法产生，但如下文所讨论的那样仍编码本发明中所公开的杀虫蛋白。由本发明包括的变体蛋白是有生物学活性的，即它们继续拥有天然蛋白的想要的生物学活性，即，保留杀虫活性。“保留活性”意指该变体将具有天然蛋白至少约30％、至少约50％、至少约70％或至少约80％的杀虫活性。用于测量杀虫活性的方法是本领域熟知的。见，例如，Czapla和Lang(1990)J.Econ.Entomol.83:2480-2485；Andrews等人(1988)Biochem.J.252:199-206；Marrone等人(1985)J.of EconomicEntomology 78:290-293；和美国专利号5,743,477，所述全部文献均通过引用方式完整地并入本文作为参考。
技术人员会进一步理解，可以通过突变本发明的核苷酸序列引入变化，从而导致编码的杀虫蛋白的氨基酸序列变化，而不改变该蛋白质的生物学活性。因而，分离的变体核酸分子可以通过将一个或多个核苷酸置换、添加或缺失导入本文公开的相应核苷酸序列来产生，从而将一个或多个氨基酸置换、添加或缺失导入所编码的蛋白质。突变可以通过标准技术如位点定向诱变法和PCR介导的诱变法导入。此类变体核苷酸序列也由本发明包括。
例如，可以在一个或多个预测的非必需氨基酸残基处进行保守性氨基酸置换。“非必需”氨基酸残基是可以被改变而偏离杀虫蛋白的野生型序列，但是不改变生物学活性的残基，而“必需”氨基酸残基是生物学活性所需要的。“保守性氨基酸置换”是其中氨基酸残基以具有相似侧链的氨基酸残基替换的置换。具有相似侧链的氨基酸残基家族已经在本领域中定义。这些家族包括具有碱性侧链的氨基酸(例如赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支的侧链的氨基酸(例如苏氨酸、缬氨酸、异亮氨酸)，以及具有芳香族侧链的氨基酸(例如酪氨酸、苯丙氨酸、色氨酸、组氨酸)。
Δ-内毒素通常具有5个保守的序列结构域和3个保守的结构性结构域(见，例如，de Maagd等人.(2001)Trends Genetics 17:193-199)。第一个保守的结构性结构域由7个α螺旋组成并且参与膜插入和孔形成。结构域II由以希腊钥构型排列的3个β-片层组成，并且结构域III由“簿卷饼型”形式的2个反平行β-片层组成(de Maagd等人,2001，上文)。结构域II和III参与受体识别和结合，并且因此被视为毒素特异性的决定因素。
可以在非保守区域进行氨基酸置换，其保留功能。通常，此类置换不对保守氨基酸残基或不对位于保守基序内部的氨基酸残基进行，鉴于此类残基对蛋白质活性而言为必需的。保守并且可能对蛋白质活性为必需的残基的实例包括例如在与本发明序列类似的或者相关的毒素的比对结果中所含的全部蛋白质之间相同的残基(例如，在同源性蛋白质的比对结果中相同的残基)。保守但可能允许保守性氨基酸置换并仍保持活性的残基的实例包括例如在与本发明氨基酸序列类似的或者相关的毒素的比对结果中所含的全部蛋白质之间仅具有保守性置换的残基(例如，在同源性蛋白质的比对结果中所含的全部蛋白质之间具有仅保守性置换的残基)。然而，本领域技术人员将会理解，功能性变体可以在保守残基中具有少量保守或非保守的改变。
备选地，变体核苷酸序列可以通过沿着全部或部分的编码序列随机导入突变(如通过饱和诱变法)产生，并且可以对得到的突变体筛选赋予杀虫活性的能力以鉴定保留活性的突变体。诱变后，可以重组地表达所编码的蛋白质，并且可以使用标准测定法技术测定该蛋白质的活性。
使用如PCR、杂交等方法，可以从样品(例如，含有核酸序列的样品，例如生物样品)中鉴定、分离或者回收相应的杀虫序列，此类序列与本发明的序列具有相当大的同一性(例如，遍及参照序列的全部范围的至少约70％、至少约75％、80％、85％、90％、95％或更多的序列同一性)并具有或者赋予杀虫活性。见，例如，Sambrook和Russell(2001)MolecularCloning:A Laboratory Manual.(Cold Spring Harbor Laboratory Press,Cold SpringHarbor,NY)，和Innis等人(1990)PCR Protocols:A Guide to Methods and Applications(Academic Press,NY)。
在杂交方法中，全部或部分的杀虫核苷酸序列可以用来筛选cDNA或基因组文库。用于构建此类cDNA和基因组文库的方法通常是本领域已知的并且在上文Sambrook和Russell，2001中公开。所谓的杂交探针可以是基因组DNA片段、cDNA片段、RNA片段或其他寡核苷酸，并且可以用可检测基团如32P或任何其他可检测标记物(如其他放射性同位素、荧光化合物、酶或酶辅因子)标记。可以通过标记以本文中公开的编码已知杀虫蛋白的核苷酸序列为基础的合成性寡核苷酸产生用于杂交的探针。可以额外地使用基于核苷酸序列或编码的氨基酸序列中保守的核苷酸或氨基酸残基设计的简并性引物。探针一般包含核苷酸序列的区域，所述的区域在严格条件下与本发明编码杀虫蛋白的核苷酸序列或其片段或变体的至少约12个、至少约25个、至少约50、75、100、125、150、175或200个连续核苷酸杂交。用于制备杂交用探针的方法通常是本领域已知的并且在通过引用方式并入本文的上文Sambrook和Russell，2001中公开。
例如，本文中公开的完整杀虫蛋白序列或其一个或多个部分可以作为能够与相应的杀虫蛋白样序列和信使RNA特异性杂交的探针使用。为在多种条件下实现特异性杂交，此类探针包括下述序列，所述序列是独特的并且优选地是至少约10个核苷酸长度或至少约20个核苷酸长度。此类探针可以用来通过PCR从所选的生物或样品扩增相应的杀虫序列。该项技术可以用来从目的生物中分离额外的编码序列或作为诊断性测定法用来确定生物中编码序列的存在。杂交技术包括铺板DNA文库的杂交筛选法(蚀斑或菌落；见，例如，Sambrook等人(1989)Molecular Cloning:A Laboratory Manual(第2版，Cold Spring HarborLaboratory Press,Cold Spring Harbor,New York)。
此类序列的杂交可以在严格性条件下实施。“严格条件”或“严格杂交条件”意指这样的条件，在所述条件下探针将会与其靶序列杂交至比其与其他序列杂交可检测性更大的程度(例如，高于背景至少2倍)。严格条件是序列依赖的并且在不同的情况下不同。通过控制杂交和/或洗涤条件的严格性，可以鉴定与探针100％互补的靶序列(同源探测)。备选地，可以调节严格性条件以允许序列中的一些错配，从而检测到较低程度的相似性(异源探测)。通常，探针是小于约1000个核苷酸长度，优选地小于500个核苷酸长度。
一般而言，严格性条件将是这些条件，其中盐浓度在pH 7.0至8.3时小于约1.5MNa离子，一般约0.01至1.0M Na离子浓度(或其他盐)并且对于短探针(例如10至50个核苷酸)，温度是至少约30℃，并且对于长探针(例如多于50个核苷酸)，温度是至少约60℃。也可以通过添加去稳定剂如甲酰胺实现严格条件。示例性低严格性条件包括在37℃用30至35％甲酰胺，1M NaCl，1％SDS(十二烷基硫酸钠)的缓冲溶液杂交，并且在50至55℃于1X至2XSSC(20X SSC＝3.0M NaCl/0.3M柠檬酸三钠)中洗涤。示例性中等严格性条件包括在37℃于40至45％甲酰胺，1.0M NaCl，1％SDS中杂交，并且在55至60℃于0.5X至1XSSC中洗涤。示例性高严格性条件包括在37℃于50％甲酰胺，1M NaCl，1％SDS中杂交，并且在60至65℃于0.1XSSC中洗涤。任选地，洗涤缓冲液可以包含约0.1％至约1％SDS。杂交的持续时间一般是小于约24小时，通常约4至约12小时。
特异性一般是杂交后洗液的函数，关键因素是终末洗涤溶液的离子强度和温度。对于DNA-DNA杂合体，Tm可以从Meinkoth和Wahl(1984)Anal.Biochem.138:267-284的等式：Tm＝81.5℃+16.6(log M)+0.41(％GC)-0.61(％form)-500/L近似算出；其中M是单价阳离子的摩尔浓度，％GC是DNA中鸟苷和胞嘧啶核苷酸的百分数，％form是杂交溶液中甲酰胺的百分数，并且L是该杂合体的碱基对长度。Tm是50％互补性靶序列与完全匹配的探针杂交的温度(在定义的离子强度和pH下)。Tm因每1％错配下降约1℃；因此，可以调节Tm、杂交和/或洗涤条件以杂交至具有目的同一性的序列。例如，如果寻求具有≥90％同一性的序列，则Tm可以降低10℃。通常，选择严格性条件比在定义的离子强度和pH下的该特定序列及其互补物的热变性温度低约5℃。然而，极严格性条件可以利用比热变性温度(Tm)低1、2、3或4℃的杂交和/或洗涤；中等严格性条件可以利用比热变性温度(Tm)低6、7、8、9或10℃的杂交和/或洗涤；低严格性条件可以利用比热变性温度(Tm)低11、12、13、14、15或20℃的杂交和/或洗涤。使用该等式、杂交与洗涤组合和想要的Tm，普通技术人员将会理解杂交和/或洗涤溶液的严格性的变化被固有地描述。如果想要的错配程度产生小于45℃(水溶液)或32℃(甲酰胺溶液)的Tm，则优选增加SSC浓度，从而可以使用更高的温度。在Tijssen(1993)Laboratory Techniques in Biochemistry and Molecular Biology—Hybridizationwith Nucleic Acid Probes,第I部分，第2章(Elsevier,New York)；和Ausubel等人编辑(1995)Current Protocols in Molecular Biology，第2章(Greene Publishing和Wiley-Interscience,New York)中存在对核酸杂交的广泛指导。见Sambrook等人(1989)Molecular Cloning:A Laboratory Manual(第2版，Cold Spring Harbor LaboratoryPress,Cold Spring Harbor,New York)。
分离的蛋白质及其变体和片段
杀虫蛋白也包括在本发明范围内。“杀虫蛋白”意图指具有SEQ ID NO:28-62中所述氨基酸序列的蛋白质。也提供了其片段、生物学活性部分和变体，并且可以用来实施本发明的方法。“分离的蛋白质”用于指不再在其天然环境中，例如在体外或者在重组的细菌或植物宿主细胞中的蛋白质。
“片段”或“生物学活性部分”包括多肽片段，它们包含与任一SEQ ID NO:28-62所述的氨基酸序列充分同一并且显示杀虫活性的氨基酸序列。杀虫蛋白的生物学活性部分可以是例如10、25、50、100个或更多氨基酸长度的多肽。此类生物学活性部分可以通过重组技术制备并评价杀虫活性。用于测量杀虫活性的方法是本领域熟知的。见，例如，Czapla和Lang(1990)J.Econ.Entomol.83:2480-2485；Andrews等人(1988)Biochem.J.252:199-206；Marrone等人(1985)J.of Economic Entomology 78:290-293；和美国专利号5,743,477，所述全部文献均通过引用方式完整并入本文。如此处使用，片段包含SEQ ID NO:28-62的至少8个连续氨基酸。然而，本发明包括其他片段，如蛋白质中多于约10、20、30、50、100、150、200、250、300、350、400、400、450、500、550、600、650、700、750、800、850、900、950、1000、1050、1100、1150、1200、1250个或1300个氨基酸的任意片段。
“变体”意指具有与任一SEQ ID NO:28-62的氨基酸序列至少约60％、65％、约70％、75％、约80％、85％、约90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一的氨基酸序列的蛋白质或多肽。变体也包括由下述核酸分子编码的多肽，其中所述核酸分子与SEQ ID NO:1-27的核酸分子或其互补物在严格条件下杂交。变体包括因诱变而在氨基酸序列上不同的多肽。由本发明包括的变体蛋白是有生物学活性的，即它们继续拥有天然蛋白的目的生物学活性，即，保留杀虫活性。在一些实施方案中，变体具有改善的活性。用于测量杀虫活性的方法是本领域熟知的。见，例如，Czapla和Lang(1990)J.Econ.Entomol.83:2480-2485；Andrews等人(1988)Biochem.J.252:199-206；Marrone等人(1985)J.of Economic Entomology 78:290-293；和美国专利号5,743,477，所述全部文献均通过引用方式完整地并入本文。
细菌基因，如本发明的axmi基因，相当经常地拥有多个靠近可读框起点的甲硫氨酸起始密码子。常常，在这些起始密码子中一个或多个起始密码子处的翻译起始将会导致功能性蛋白质的产生。这些起始密码子可以包括ATG密码子。然而，细菌(如芽孢杆菌属物种)也将密码子GTG识别为起始密码子，并且在GTG密码子处启动翻译的蛋白质含有在第一氨基酸处的甲硫氨酸。在个别情况下，细菌系统中的翻译可以在TTG密码子处启动，不过在该情况下TTG编码甲硫氨酸。另外，常常不推理地确定在细菌中天然地使用这些密码子中的哪些密码子。因而，可以理解使用可选甲硫氨酸密码子中的一个甲硫氨酸密码子也可以导致杀虫蛋白的产生。在本发明中包括这些杀虫蛋白并且它们可以在本发明的方法中使用。应当理解，当在植物中表达时，必须将备选的起始密码子改变成ATG，以正确地翻译。
本发明也包括针对本发明多肽或针对其变体或片段的抗体。用于产生抗体的方法是本领域熟知的(见，例如，Harlow和Lane(1988)Antibodies:A Laboratory Manual,ColdSpring Harbor Laboratory,Cold Spring Harbor,NY；美国专利号4,196,265)。
改变或改善的变体
认识到可以通过多种方法改变杀虫蛋白的DNA序列，并且这些改变可以产生编码蛋白质的DNA序列，其中所述的蛋白质具有与本发明杀虫蛋白所编码的氨基酸序列不同的氨基酸序列。这个蛋白质可以以多种方式改变，所述方式包括SEQ ID NO:28-62的一个或多个氨基酸的氨基酸置换、缺失、截短和插入，包括多达约2、约3、约4、约5、约6、约7、约8、约9、约10、约15、约20、约25、约30、约35、约40、约45、约50、约55、约60、约65、约70、约75、约80、约85、约90、约100、约105、约110、约115、约120、约125、约130个或更多个氨基酸置换、缺失或插入。
用于此类操作的方法通常是本领域已知的。例如，可以通过在DNA中突变来制备杀虫蛋白的氨基酸序列变体。这也可以通过几种形式的诱变法之一和/或在定向进化中完成。在一些方面中，氨基酸序列中所编码的变化基本上不会影响蛋白质的功能。此类变体会拥有目的杀虫活性。然而，可以理解，杀虫蛋白赋予杀虫活性的能力可以通过使用此类技术至本发明的组合物来改善。例如，可以在DNA复制期间显示高碱基错误掺入率的宿主细胞如XL-1Red(Stratagene)中表达杀虫蛋白。在此类菌株中增殖后，可以分离毒素DNA(例如通过制备质粒DNA或通过PCR扩增并将所得的PCR片段克隆至载体中)，在非诱变性菌株中培养毒素突变体，并且鉴定具有杀虫活性的突变毒素基因，例如通过实施检测杀虫活性的测定法。一般而言，将所述蛋白质混合并用于饲喂测定法中。见，例如Marrone等人(1985)J.ofEconomic Entomology 78:290-293。此类测定法可以包括使植物与一种或多种害虫接触并且确定植物存活和/或造成害虫死亡的能力。导致毒性增加的突变的实例存在于Schnepf等人(1998)Microbiol.Mol.Biol.Rev.62:775-806中。
备选地，可以在氨基或羧基端改变许多蛋白质的蛋白质序列，而基本上不影响活性。这可以包括通过现代分子方法(如PCR)导入的插入、缺失或改变，所述的PCR包括通过在PCR扩增中所用的寡核苷酸中纳入氨基酸编码序列而改变或延伸蛋白质编码序列的PCR扩增。备选地，所添加的蛋白质序列可以包括完整的蛋白质编码序列，如本领域常见用来产生蛋白质融合物的那些序列。此类融合蛋白常常用来(1)增加目的蛋白的表达，(2)导入结合结构域、酶活性或表位来促进蛋白质纯化、蛋白质检测或本领域已知的其他实验用途，(3)将蛋白质靶向分泌或翻译至亚细胞器，如革兰阴性细菌的周质间隙或真核细胞的内质网，后者常常导致蛋白质的糖基化。
本发明的变体核苷酸和氨基酸序列也包括从诱变性和重组方法如DNA改组法衍生的序列。采用这种方法，一种或多种不同的杀虫蛋白编码区可以用来产生拥有想要的特性的新杀虫蛋白。以这种方式，从包含具有相当高序列同一性并且可以在体外或体内同源重组的序列区的相关性序列多核苷酸群产生重组多核苷酸的文库。例如，使用这种方法，可以改组本发明的杀虫基因和其他已知的杀虫基因之间编码目的结构域的序列基序以获得编码蛋白质的新基因，其中所述蛋白质具有改善的目的特性，如增加的杀昆虫活性。用于这种DNA改组的策略是本领域已知的。见，例如Stemmer(1994)Proc.Natl.Acad.Sci.USA 91:10747-10751；Stemmer(1994)Nature 370:389-391；Crameri等人(1997)NatureBiotech.15:436-438；Moore等人(1997)J.Mol.Biol.272:336-347；Zhang等人(1997)Proc.Natl.Acad.Sci.USA 94:4504-4509；Crameri等人(1998)Nature 391:288-291；和美国专利号5,605,793和5,837,458。
结构域交换或改组是用于产生改变的Δ-内毒素蛋白的另一种机制。结构域可以是在Δ-内毒素蛋白之间交换，从而产生具有改善的杀虫活性或靶谱的杂合体或嵌合毒素。用于产生重组蛋白和测试其杀虫活性的方法是本领域熟知的(见，例如，Naimov等人(2001)Appl.Environ.Microbiol.67:5328-5330；de Maagd等人(1996)Appl.Environ.Microbiol.62:1537-1543；Ge等人(1991)J.Biol.Chem.266:17954-17958；Schnepf等人(1990)J.Biol.Chem.265:20923-20930；Rang等人91999)Appl.Environ.Microbiol.65:2918-2925)。
又在另一实施方案中，可以使用错配PCR、针对寡核苷酸的诱变、组装PCR、有性PCR诱变、体内诱变、盒诱变、递归整体诱变、指数整体诱变、位点特异的诱变、基因重装配、基因位点饱和诱变、全突变诱变(permutational mutagenesis)、合成的连接重装配(SLR)、重组、递归序列重组、硫代磷酸酯修饰的DNA诱变、含尿嘧啶模板的诱变、缺口双链体诱变、点错配修复诱变、修复缺陷型宿主菌株诱变、化学诱变、放射所致的诱变、缺失诱变、限制选择诱变、限制纯化诱变、人工基因合成、整体诱变、嵌合的核酸多聚体产生等等中的一种或多种获得变体核苷酸和/或氨基酸序列。
载体
可以在用于目的植物中表达的表达盒中提供本发明的杀虫序列。“植物表达盒”意指能够在植物细胞中导致蛋白质从可读框中表达的DNA构建体。一般地，这些植物表达盒含有启动子和编码序列。常常地，此类构建体也会含有3′非翻译区。此类构建体可以含有促进该肽共翻译或翻译后运输至某些胞内结构如叶绿体(或其他质体)、内质网或高尔基体的“信号序列”或“前导序列”。
“信号序列”意指已知或推测导致跨细胞膜共翻译或翻译后肽运输的序列。在真核生物中，这一般涉及分泌至高尔基体中，伴有所得的一些糖基化。通常将细菌的杀昆虫毒素合成为前毒素，其在靶害虫的肠中蛋白水解(protolytically)活化(Chang(1987)MethodsEnzymol.153：507-516)。在本发明的一些实施方案中，信号序列定位于天然序列中，或者可以来源于本发明的序列。“前导序列”意指任意的下述序列，所述序列在翻译时产生足以触发肽链共翻译性运输至亚细胞器的氨基酸序列。因而，这包括通过进入内质网、进入液泡、质体(包括叶绿体)、线粒体等引导运输和/或糖基化的前导序列。
“植物转化载体”意指用于有效转化植物细胞必需的DNA分子。这种分子可以由一个或多个植物表达盒组成，并且可以组织成多于一个“载体”DNA分子。例如，双元载体是使用两个非连续DNA载体来编码用于转化植物细胞的全部必要顺式和反式作用功能的植物转化载体(Hellens和Mullineaux(2000)Trends in Plant Science 5:446-451)。“载体”指为不同宿主细胞之间转移所设计的核酸构建体。“表达载体”指在外来细胞中具有并入、整合和表达异源DNA序列或片段的能力的载体。该盒将包括与本发明序列有效连接的5'和3'调节序列。“有效连接”意指启动子与第二序列之间的功能性连接，其中所述启动子序列启动并且介导与第二序列相对应的DNA序列的转录。通常，有效连接意指被连接的核酸序列是连续的，并且在需要连接两个蛋白质编码区的情况下，是连续的并且处于相同的可读框中。该盒可以额外地含有至少一个待共转化入生物中的额外基因。备选地，可以在多个表达盒上提供额外的基因。
“启动子”指发挥指导下游编码序列转录的功能的核酸序列。对于目的DNA序列的表达而言，启动子连同其他转录性和翻译性调节核酸序列(又称作“调控序列”)是必需的。
这种表达盒提供有用于插入处在调节区转录性调控下的杀虫序列的多个限制性位点。
所述表达盒将会在5’-3’转录方向包括在植物中有功能的转录和翻译起始区(即，启动子)、本发明的DNA序列和转录与翻译终止区(即，终止区)。启动子可以是天然的或类似的，或对植物宿主和/或对本发明的DNA序列是外来或异源的。另外，启动子可以是天然序列或备选地是合成序列。在启动子对植物宿主是“天然”或“同源”的情况下，意指该启动子存在于导入该启动子的天然植物中。在启动子对本发明的DNA序列是“外来”或“异源”的情况下，意指该启动子对于有效连接的本发明DNA序列而言不是本来的或天然存在的启动子。
终止区可以相对于转录起始区是固有的，可以相对于有效连接的目的DNA序列是固有的，可以相对于植物宿主是固有的，或可以从另外来源衍生(即，相对于启动子、目的DNA序列、植物宿主或其任意组合是外来或异源的)。常规的终止区是从根瘤农杆菌(A.tumefaciens)的Ti质粒可得到的，如章鱼碱合酶和胭脂碱合成酶的终止区。也见Guerineau等人(1991)Mol.Gen.Genet.262:141-144；Proudfoot(1991)Cell 64:671-674；Sanfacon等人(1991)Genes Dev.5:141-149；Mogen等人(1990)Plant Cell 2:1261-1272；Munroe等人(1990)Gene 91:151-158；Ballas等人(1989)Nucleic Acids Res.17:7891-7903；和Joshi等人(1987)Nucleic Acid Res.15:9627-9639。
根据需要，基因可以优化为在转化的宿主细胞中增加的表达。即，基因可以为了改善的表达而使用宿主细胞优选的密码子合成，或可以以宿主优选的密码子选择频率使用密码子加以合成。通常，基因的GC含量将会增加。见，例如，Campbell和Gowri(1990)PlantPhysiol.92:1-11对宿主优选密码子使用的讨论。用于合成植物优选基因的方法是本领域可获得的。见，例如，通过引用的方式并入本文的美国专利号5,380,831和5,436,391以及Murray等人,1989,Nucleic Acids Res.17:477-498。
在一个实施方案中，将杀虫序列靶向至叶绿体用于表达。以这种方式，在杀虫序列不直接插入叶绿体的情况下，表达盒将额外地含有这样的核酸，其编码指引杀虫序列至叶绿体的转运肽。此类转运肽是本领域已知的。见，例如，Von Heijne等人(1991)PlantMol.Biol.Rep.9:104-126；Clark等人(1989)J.Biol.Chem.264:17544-17550；Della-Cioppa等人(1987)Plant Physiol.84:965-968；Romer等人(1993)Biochem.Biophys.Res.Commun.196:1414-1421；和Shah等人(1986)Science 233:478-481。
待靶向至叶绿体的杀虫基因可以为了在叶绿体中表达进行优化以解决植物细胞核和这种细胞器之间密码子选择的差异。以这种方式，目的核酸可以使用叶绿体优选的密码子来合成。见，例如，通过引用方式并入本文的美国专利号5,380,831。
植物转化
本发明的方法涉及导入核苷酸构建体至植物中。术语“导入”意指如此方式向植物呈递核苷酸构建体，从而该构建体进入该植物的细胞的内部。本发明的方法不要求使用将核苷酸构建体导入植物中的特定方法，只要该核苷酸构建体进入植物的至少一个细胞的内部即可。用于将核苷酸构建体导入植物中的方法是本领域已知的，包括但不限于稳定转化法、瞬时转化法和病毒介导法。
“植物”意指完整植物、植物器官(例如，叶、茎、根等)、种子、植物细胞、繁殖体、胚和其子代。植物细胞可以是分化的或未分化的(例如愈伤组织、悬浮培养细胞、原生质体、叶细胞、根细胞、韧皮部细胞、花粉)。
“转基因植物”或“转化的植物”或“稳定转化的”植物或细胞或组织指已经使外源核酸序列或DNA片段并入或整合入植物细胞中的植物。这些核酸序列包括外源的或在未转化的植物细胞中不存在的那些核酸序列以及可以内源或在未转化的植物细胞中存在的那些核酸序列。“异源的”通常指这些核酸序列，所述核酸序列相对于它们存在其中的细胞或天然基因组的部分而言不为内源并且已经通过感染法、转染法、微量注射法、电穿孔法、微量投射法等添加至细胞。
本发明的转基因植物表达本文中公开的一种或多种杀虫序列。在多个实施方案中，转基因植物还包含一个或多个用于昆虫抵抗性的额外基因，例如，一个或多个用于控制鞘翅目的、鳞翅目的、异翅目的或线虫类害虫的额外基因。本领域技术人员将会理解该转基因植物可以包含赋予目的农学性状的任意基因。
植物细胞的转化可以通过本领域已知的几项技术之一完成。可以修饰本发明的杀虫基因以获得或增强在植物细胞中的表达。一般而言，表达这种蛋白质的构建体含有驱动该基因转录的启动子以及允许转录终止和多腺苷酸化的3′非翻译区。此类构建体的组织构造是本领域熟知的。在一些情况下，可能有用的是使该基因工程化，从而所得的肽被分泌或靶向于植物细胞内部。例如，该基因可以工程化以含有促进肽转移至内质网的信号肽。也可以优选的是工程化植物表达盒以含有内含子，从而对表达而言需要内含子的mRNA加工。
一般而言，这种“植物表达盒”将插入“植物转化载体”中。这种植物转化载体可以包含实现植物转化所需要的一个或多个DNA载体。例如，本领域中常见的实践是使用由多于一个连续DNA节段组成的植物转化载体。这些载体在本领域常常称作“双元载体”。双元载体以及带有辅助质粒的载体最常用于农杆菌介导的转化，其中为实现高效转化所需要的DNA节段的大小和复杂度是相当大的，并且有利的是将多项功能分配至独立的DNA分子上。双元载体一般含有这样的质粒载体，其含有为T-DNA转移所需要的顺式作用序列(如左边界和右边界)、工程化以能够在植物细胞中表达的选择标记和“目的基因”(工程化以能够在对于产生转基因植物所需要的植物细胞中表达的基因)。在这种质粒载体上也存在为细菌复制所需要的序列。顺式作用序列以允许高效转移至植物细胞中并在其中表达的方式排列。例如，选择标记基因和杀虫序列位于左边界和右边界之间。第二种质粒载体经常含有介导T-DNA从农杆菌转移至植物细胞的反式作用因子。如本领域理解，这种质粒经常含有允许农杆菌感染植物细胞的毒力功能(Vir基因)和通过在边界序列处切割的DNA的转移和vir介导的DNA转移(Hellens和Mullineaux(2000)Trends in Plant Science 5:446-451)。几个类型的农杆菌菌株(例如LBA4404、GV3101、EHA101、EHA105等)可以用于植物转化。第二质粒载体对于通过其他方法如微量投射法、微量注射法、电穿孔法、聚乙二醇法等转化植物而言不是必需的。
通常，植物转化方法涉及将异源DNA转移至靶植物细胞中(例如不成熟或成熟的胚、悬浮培养物、未分化的愈伤组织、原生质体等)，随后施加最大阈水平的适宜选择(取决于选择标记基因)以从一组未转化的细胞团回收转化的植物细胞。一般将外植体转移至相同培养基的新鲜补给物并且例行地培养。随后，转化的细胞在补充有最大阈水平选择剂的再生培养基上放置后分化成苗。苗随后转移至选择性生根培养基用于回收生根的苗或小植株。转基因小植株随后生长为成熟植物并且产生能育性种子(例如Hiei等人(1994)ThePlant Journal 6:271-282；Ishida等人(1996)Nature Biotechnology 14:745-750)。一般将外植体转移至相同培养基的新鲜补给物并且例行地培养。对产生转基因植物的技术和方法的一般描述存在于Ayres和Park(1994)Critical Critical Reviews in Plant Science13:219-239以及Bommineni和Jauhar(1997)Maydica 42:107-120中。由于转化的材料含有许多细胞；转化和未转化的细胞均存在于任何一片受处理的靶愈伤组织或组织或细胞群中。杀死未转化细胞并且允许转化细胞增殖的能力产生转化的植物培养物。除去未转化细胞的能力经常是快速回收转化的植物细胞和成功产生转基因植物的限制。
转化方案以及用于将核苷酸序列导入植物中的方案可以根据用于转化的植物或植物细胞的类型(即单子叶植物或双子叶植物)变化。可以通过几种方法之一进行转基因植物的产生，所述方法包括但不限于微量注射法、电穿孔法、直接基因转移法、通过农杆菌导入异源DNA至植物细胞中(农杆菌介导的转化法)、用粘附至粒子的异源外来DNA轰击植物细胞、射弹粒子加速法、气溶胶束转化法(美国公开的申请号20010026941；美国专利号4,945,050；国际公开号WO 91/00915；美国公开的申请号2002015066)、Lec1转化法和转移DNA的多种其他的非粒子直接介导方法。
用于转化叶绿体的方法是本领域已知的。见，例如，Svab等人,(1990),Proc.Natl.Acad.Sci.USA 87:8526-8530；Svab和Maliga,(1993),Proc.Natl.Acad.Sci.USA 90:913-917；Svab和Maliga,(1993),EMBO J.12:601-606。该方法依赖于粒子枪递送含有选择性标记的DNA和通过同源重组导引该DNA至质体基因组。额外地，质体转化可以通过组织优选性表达细胞核编码且指向质体的RNA聚合酶反式地激活质体携带的沉默转基因来实现。这种系统已经在McBride等人,(1994),Proc.Natl.Acad.Sci.USA 91:7301-7305中报道。
在异源外来DNA整合至植物细胞中之后，随后在培养基中施加最大阈水平的适宜选择以通过定期转移至新鲜培养基而杀死未转化的细胞并且分离和增殖从这种选择处理中存活的推定的转化细胞。通过连续传代并且用适宜的选择攻击，鉴定并且增殖用质粒载体转化的细胞。随后可以使用分子方法和生物化学方法来证实存在整合至转基因植物基因组中的异源目的基因。
已经转化的细胞可以根据常规方法培育成植物。见，例如McCormick等人(1986)Plant Cell Reports 5:81-84。这些植物随后可以进行培育，并且用相同的转化株系或不同的株系授粉，并且鉴定具有所需要表型特征组成型表达的所得杂交种。可以培育两个或多个世代以确保稳定维持并且遗传了所需要表型特征的表达，并且随后可以收获种子以确保已经实现所需要表型特征的表达。以这种方式，本发明提供了使本发明的核苷酸构建体(例如本发明的表达盒)稳定并入其基因组中的转化种子(也称作“转基因种子”)。
植物转化的评价
在导入异源外来DNA至植物细胞中之后，通过多种方法如分析与所整合基因相关的核酸、蛋白质和代谢物来证实异源基因在植物基因组中的转化或整合。
PCR分析是在移植入土壤之前的早期对转化的细胞、组织或苗筛选所并入基因存在的快速方法(Sambrook和Russell(2001)Molecular Cloning:A LaboratoryManual.Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY)。使用针对目的基因或农杆菌载体背景等特异的寡核苷酸引物实施PCR。
可以通过基因组DNA的DNA印迹分析证实植物转化(上文Sambrook和Russell,2001)。通常，从转化体提取总DNA，用适宜的限制酶消化，在琼脂糖凝胶中分离并且转移至硝化纤维素或尼龙膜。膜或“印迹物”随后根据标准技术(上文Sambrook和Russell,2001)用例如放射标记的32P靶DNA片段探测以证实所导入基因整合到植物基因组中。
在RNA印迹分析中，从转化体的特定组织分离RNA，在甲醛琼脂糖凝胶中分离并且根据本领域例行所用的标准方法(上文Sambrook和Russell,2001)印迹至尼龙滤膜上。随后通过本领域已知方法(上文Sambrook和Russell,2001)使该滤膜与从毒素基因衍生的放射活性探针杂交，检验由杀虫序列编码的RNA的表达。
可以对转基因植物实施蛋白质印迹法、生物化学测定法等以使用与杀虫蛋白上存在的一个或多个表位结合的抗体，通过标准方法(上文Sambrook和Russell,2001)证实由杀虫基因编码的蛋白质的存在。
植物中的杀虫活性
在本发明的另一个方面，可以产生表达具有杀虫活性的毒素的转基因植物。上文通过例举方式所述的方法可以用来产生转基因植物，但是产生转基因植物细胞的方式对于本发明并非关键。本领域已知或描述的方法，如农杆菌介导的转化法、生物射弹转化法和非粒子介导方法，可以由实验者酌情使用。表达杀虫序列的植物可以通过本领域描述的常见方法予以分离，例如通过转化愈伤组织、选择转化的愈伤组织和从此类转基因愈伤组织再生能育植物。在这个方法中，可以使用任意基因作为选择标记，只要该选择标记在植物细胞中的表达赋予鉴定或选择转化细胞的能力。
已经开发了随植物细胞一起使用的众多标记，如氯霉素、氨基糖甙G418、潮霉素等抗性标记。也可以使用编码参与叶绿体代谢的产物的其他基因作为选择标记。例如，提供针对植物除草剂如草甘膦、溴苯腈或咪唑啉酮的抗性的基因可能特别有用。已经报道了此类基因(Stalker等人(1985)J.Biol.Chem.263:6310-6314(溴苯腈抗性腈水解酶基因)；和Sathasivan等人(1990)Nucl.Acids Res.18:2188(AHAS咪唑啉酮抗性基因)。额外地，本文中公开的基因用作标记以评估细菌细胞或植物细胞的转化。用于检测转基因在植物、植物器官(例如，叶、茎、根等)、种子、植物细胞、繁殖体、胚或其子代中存在的方法是本领域熟知的。在一个实施方案中，通过测试杀虫活性检测该转基因的存在。
可以对表达杀虫序列的能育植物测试杀虫活性，并且选择显示最佳活性的植物用于进一步育种。检测杀虫活性的方法是本领域可获得的。一般而言，将所述蛋白质混合并用于饲喂测定法中。见，例如Marrone等人(1985)J.of Economic Entomology 78:290-293。
本发明可以用于转化任何植物物种，包括但不限于单子叶植物和双子叶植物。目的植物的实例包括但是不限于谷物(玉米)、高粱、小麦、向日葵、番茄、十字花科植物、胡椒属植物、马铃薯、棉花、稻、大豆、甜菜、甘蔗、烟草、大麦和油籽油菜、芸苔属物种(Brassicasp.)、苜蓿、黑麦、粟、红花、花生、甘薯、木薯、咖啡、椰子、凤梨、柑桔树、可可、茶、香蕉、鳄梨、无花果、番石榴、芒果、油橄榄、番木瓜、腰果、全缘叶澳洲坚果、扁桃、燕麦、蔬菜类、观赏植物类和松柏类。
蔬菜包括但是不限于番茄、生菜、青豆、利马豆、豌豆和黄瓜属(Cucumis)的成员如黄瓜、网纹甜瓜和香甜瓜。观赏植物包括但是不限于杜鹃花、八仙花、朱槿、玫瑰、郁金香、水仙、矮牵牛、康乃馨、一品红和菊花。优选地，本发明的植物是作物植物(例如，玉米、高粱、小麦、向日葵、番茄、十字花科植物、胡椒属植物、马铃薯、棉花、稻、大豆、甜菜、甘蔗、烟草、大麦、油籽油菜等)。
在杀虫控制中的用途
将包含本发明核苷酸序列或其变体的菌株应用于杀虫剂控制中或工程化其他生物作为杀虫剂中的一般方法是本领域已知的。见，例如，美国专利号5,039,523和EP0480762A2。
含有本发明核苷酸序列或其变体的芽孢杆菌属菌株或已经遗传地改变以含有杀虫基因和蛋白质的微生物可以用于保护农作物和农产品免遭害虫影响。在本发明的一个方面，产毒素(杀虫剂)生物的完整(即未裂解)细胞用下述试剂处理，其中在应用该细胞至靶害虫的环境时，所述的试剂延长了细胞中所产生杀虫蛋白的活性。
备选地，通过导入杀虫基因至细胞性宿主中产生杀虫剂。杀虫基因的表达直接或间接地导致该杀虫剂的胞内产生和维持。在本发明的一个方面，这些细胞随后在下述条件下处理，其中在应用该细胞至靶害虫的环境时，所述的条件延长了细胞中所产生杀虫的活性。所得的产物保留杀虫蛋白的毒性。这些天然包囊化的杀虫剂随后可以根据常规技术配制以应用至靶害虫所在的环境，例如，土壤、水和植物的叶。见，例如EPA 0192319及其中援引的参考文献。备选地，可以配制表达本发明基因的细胞，从而允许应用所得的物质作为杀虫剂。
杀虫组合物
本发明的活性成分通常以组合物形式应用并且可以随其他复合物同时或依次地应用至待处理的作物区域或植物。这些复合物可以是肥料、除草剂、抗冻剂、表面活性剂、去垢剂、杀虫皂、休眠油、聚合物和/或在单次应用该制剂后允许对靶区域长期投药的延时释放或生物可降解载体制剂。它们也可以是选择性除草剂、化学杀虫剂、杀病毒剂、杀微生物剂、抗阿米巴药、杀虫剂、杀真菌剂、杀细菌剂、杀线虫药、杀螺剂或这些制品中几种制品的混合物，根据需要，连同制剂领域通常使用的其他农药可接受载体、表面活性剂或应用促进佐剂。合适的载体和佐剂可以是固体或液体并且对应于制剂技术中通常使用的物质，例如天然或再生性矿物质、溶剂、分散剂、润湿剂、增粘剂、粘合剂或肥料。同样地，制剂可以制备成可食性“饵料”或加工成害虫“陷阱”以允许靶害虫采食或摄取杀虫制剂。
应用本发明活性成分或本发明农业化学组合物的方法包括叶面应用、种子包衣和土壤应用，其中所述的本发明活性成分或农业化学组合物含有少一种由本发明细菌菌株产生的杀虫蛋白。应用的次数和应用的速率取决于相应害虫的侵袭强度。
该组合物可以作为粉剂、尘剂、丸剂、粒剂、喷雾剂、乳剂、胶体剂、溶液剂等配制，并且可以通过常规手段如干燥、冻干、均化、提取、过滤、离心、沉淀或浓缩包含所述多肽的细胞的培养物来制备。在含有至少一种所述杀虫多肽的全部此类组合物中，该多肽可以以按重量计约1％至约99％的浓度存在。
可以通过本发明的方法杀死给定地区内的鳞翅目害虫、双翅目害虫、异翅目害虫、鞘翅目害虫或线虫类害虫或减少其数目，或可以预防地应用至某环境区域以防止易感害虫侵袭。优选地，害虫摄取或接触杀虫有效量的所述多肽。“杀虫有效量”意指杀虫剂的能够对至少一种害虫造成死亡或能够显著减少害虫生长、采食或正常生理发育的量。该量将根据此类因素变动，例如，待控制的特定靶害虫、待处理的具体环境、位置、植物、作物或农业地点、环境条件，和应用杀虫有效的多肽组合物的方法、速率、浓度、稳定性及量。所述制剂也可以根据气候条件、环境考虑和/或应用频率和/或害虫侵袭的严重性方面而变动。
可以通过将细菌细胞、晶体和/或孢子悬液或分离的蛋白质组分与想要的可农用载体配制来制造所述杀虫剂组合物。所述组合物可以在施用之前以适宜手段如冻干、冷冻干燥、干燥或在水性载体、培养基或合适的稀释剂(如盐水或其他缓冲液)中配制。配制的组合物可以是尘剂或颗粒物质形式或是在油(植物油或矿物油)中的混悬剂或水或油/水乳剂，或作为可湿性粉剂，或与适用于农业应用的任何其他载体物质组合。合适的农用载体可以是固体或液体并且是本领域熟知的。术语“可农用载体”涵盖杀虫剂配制技术中通常使用的全部佐剂、惰性组分、分散剂、表面活性剂、增粘剂、粘合剂等；这些物质是杀虫剂配制领域技术人员熟知的。所述制剂可以与一种或多种固体或液体佐剂混合并且通过多种手段制备，例如，通过使用常规配制技术将杀虫性组合物与合适佐剂均匀地混合、掺合和/或研磨。合适的配制和应用方法在通过引用方式并入本文的美国专利号6,468,523中描述。
植物也可以用一种或多种化学组合物处理，所述的化学组合物包括一种或多种除草剂、杀虫剂或杀真菌剂。示例性化学组合物包括：水果/蔬菜除草剂：莠去津(Atrazine)、除草定(Bromacil)、敌草隆(Diuron)、草甘膦(Glyphosate)、利谷隆(Linuron)、嗪草酮(Metribuzin)、西玛津(Simazine)、氟乐灵(Trifluralin)、吡氟禾草灵(Fluazifop)、草铵膦(Glufosinate)、氯吡嘧磺隆(Halosulfuron Gowan)、百草枯(Paraquat)、炔草胺(Propyzamide)、稀禾定(Sethoxydim)、氟丙嘧草酯(Butafenacil)、氯吡嘧磺隆(Halosulfuron)、Indaziflam；水果/蔬菜杀虫剂：涕灭威(Aldicarb)、苏云金芽孢杆菌(Bacillus thuriengiensis)、甲萘威(Carbaryl)、克百威(Carbofuran)、毒死蜱(Chlorpyrifos)、氯氰菊酯(Cypermethrin)、溴氰菊酯(deltamethrin)、地亚农(Diazinon)、马拉硫磷(Malathion)、阿维菌素(Abamectin)、氟氯氰菊酯/β-氟氯氰菊酯(Cyfluthrin/Beta-Cyfluthrin)、顺式氰戊菊酯(Esfenvalerate)、高三氟氯氰菊酯(Lambda-cyhalothrin)、灭螨醌(Acequinocyl)、联苯肼酯(Bifenazate)、甲氧虫酰肼(Methoxyfenozide)、双苯氟脲(Novaluron)、环虫酰肼(Chromafenozide)、噻虫啉(Thiacloprid)、呋虫胺(Dinotefuran)、嘧螨酯(fluacrypyrim)、唑虫酰胺(Tolfenpyrad)、可尼丁(Clothianidin)、螺螨酯(spirodiclofen)、γ-氯氟氰菊酯(gama-cyhalothrin)、螺甲螨酯(spiromesifen)、艾克敌(spinosad)、氯虫苯甲酰胺(Rynaxypyr)、溴氰虫酰胺(Cyazypyr)、乙基多杀菌素(Spinoteram)、杀铃脲(Triflumuron)、螺虫乙酯(Spirotetramat)、吡虫啉(Imidacloprid)、氟虫双酰胺(Flubendiamide)、硫双威(Thiodicarb)、氰氟虫腙(Metaflumizone)、氟啶虫胺腈(Sulfoxaflor)、丁氟螨酯(Cyflumetofen)、Cyanopyrafen、吡虫啉(Imidacloprid)、可尼丁(Clothianidin)、噻虫嗪(Thiamethoxam)、Spinotoram、硫双威(Thiodicarb)、氟啶虫酰胺(Flonicamid)、甲硫威(Methiocarb)、氨基阿维菌素苯甲酸盐(Emamectin-benzoate)、二唑虫(Indoxacarb)、Fozthiazate、苯线磷(Fenamiphos)、硫线磷(Cadusaphos)、吡丙醚(Pyriproxifen)、苯丁锡(Fenbutatin-oxid)、噻螨酮(Hexthiazox)、灭多威(Methomyl)、4-[[(6-氯吡啶-3-基)甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮；水果/蔬菜杀真菌剂：多菌灵(Carbendazim)、百菌清(Chlorothalonil)、EBDC、硫、甲基硫菌灵(Thiophanate-methyl)、嘧菌酯(Azoxystrobin)、霜脲氰(Cymoxanil)、氟啶胺(Fluazinam)、福賽得(Fosetyl)、异菌脲(Iprodione)、醚菌酯(Kresoxim-methyl)、甲霜灵/精甲霜灵(Metalaxyl/mefenoxam)、肟菌酯(Trifloxystrobin)、噻唑菌胺(Ethaboxam)、丙森锌(Iprovalicarb)、肟菌酯(Trifloxystrobin)、环酰菌胺(Fenhexamid)、延胡索酸口恶咪唑(Oxpoconazolefumarate)、氰霜唑(Cyazofamid)、咪唑菌酮(Fenamidone)、苯酰菌胺(Zoxamide)、啶氧菌酯(Picoxystrobin)、吡唑醚菌酯(Pyraclostrobin)、环氟菌胺(Cyflufenamid)、啶酰菌胺(Boscalid)；谷类植物除草剂：异丙隆(Isoproturon)、溴苯腈(Bromoxynil)、碘苯腈(Ioxynil)、苯氧基类(Phenoxies)、氯磺隆(Chlorsulfuron)、炔草酸(Clodinafop)、禾草灵酸(Diclofop)、吡氟草胺(Diflufenican)、口恶唑禾草灵(Fenoxaprop)、双氟磺草胺(Florasulam)、氟草烟(Fluroxypyr)、甲磺隆(Metsulfuron)、醚苯磺隆(Triasulfuron)、氟酮磺隆(Flucarbazone)、碘甲磺隆(Iodosulfuron)、丙苯磺隆(Propoxycarbazone)、氟吡酰草胺(Picolinafen)、甲磺胺磺隆(Mesosulfuron)、氟丁酰草胺(Beflubutamid)、唑啉草酯(Pinoxaden)、酰嘧磺隆(Amidosulfuron)、噻磺隆(Thifensulfuron)、苯磺隆(Tribenuron)、氟啶嘧磺隆(Flupyrsulfuron)、磺酰磺隆(Sulfosulfuron)、Pyrasulfotole、甲氧磺草胺(Pyroxsulam)、氟噻草胺(Flufenacet)、肟草酮(Tralkoxydim)、Pyroxasulfon；谷类植物杀真菌剂：多菌灵(Carbendazim)、百菌清(Chlorothalonil)、嘧菌酯(Azoxystrobin)、环丙唑醇(Cyproconazole)、嘧菌环胺(Cyprodinil)、丁苯吗啉(Fenpropimorph)、氟环唑(Epoxiconazole)、醚菌酯(Kresoxim-methyl)、苯氧喹啉(Quinoxyfen)、戊唑醇(Tebuconazole)、肟菌酯(Trifloxystrobin)、硅氟唑(Simeconazole)、啶氧菌酯(Picoxystrobin)、吡唑醚菌酯(Pyraclostrobin)、醚菌胺(Dimoxystrobin)、丙硫菌唑(Prothioconazole)、氟嘧菌酯(Fluoxastrobin)；谷类植物杀虫剂：乐果(Dimethoate)、三氟氯氰菊酯(Lambda-cyhalthrin)、溴氰菊酯(Deltamethrin)、α-氯氰菊酯(alpha-Cypermethrin)、β-氟氯氰菊酯(β-Cyfluthrin)、联苯菊酯(Bifenthrin)、吡虫啉(Imidacloprid)、可尼丁(Clothianidin)、噻虫嗪(Thiamethoxam)、噻虫啉(Thiacloprid)、啶虫脒(Acetamiprid)、呋虫胺(Dinetofuran)、Clorphyriphos、甲胺磷(Metamidophos)、乙酰甲胺磷(Oxidemethon-methyl)、抗蚜威(Pirimicarb)、甲硫威(Methiocarb)；玉米除草剂：莠去津(Atrazine)、甲草胺(Alachlor)、溴苯腈(Bromoxynil)、乙草胺(Acetochlor)、麦草畏(Dicamba)、二氯吡啶酸(Clopyralid)、二甲吩草胺(S-)Dimethenamid)、草铵膦(Glufosinate)、草甘膦(Glyphosate)、异唑草酮(Isoxaflutole)、精异丙甲草胺(S-Metolachlor)、甲基磺草酮(Mesotrione)、烟嘧磺隆(Nicosulfuron)、氟嘧磺隆(Primisulfuron)、玉嘧磺隆(Rimsulfuron)、磺草酮(Sulcotrione)、甲酰胺磺隆(Foramsulfuron)、苯吡唑草酮(Topramezone)、Tembotrione、苯嘧磺草胺(Saflufenacil)、酮脲磺草吩(Thiencarbazone)、氟噻草胺(Flufenacet)、Pyroxasulfon；玉米杀虫剂：克百威(Carbofuran)、毒死蜱(Chlorpyrifos)、联苯菊酯(Bifenthrin)、氯虫双酰胺(Cyazypyr)、氟虫腈(Fipronil)、吡虫啉(Imidacloprid)、高三氟氯氰菊酯(Lambda-Cyhalothrin)、七氟菊酯(Tefluthrin)、特丁硫磷(Terbufos)、噻虫嗪(Thiamethoxam)、可尼丁(Clothianidin)、螺甲螨酯(spiromesifen)、氟虫双酰胺(Flubendiamide)、杀铃脲(Triflumuron)、氯虫苯甲酰胺(Rynaxypyr)、溴氰菊酯(Deltamethrin)、硫双威(Thiodicarb)、β-氟氯氰菊酯(β-Cyfluthrin)、氯氰菊酯(Cypermethrin)、联苯菊酯(Bifenthrin)、虱螨脲(Lufenuron)、杀虫隆(Triflumoron)、七氟菊酯(Tefluthrin)、丁基嘧啶磷(Tebupirimphos)、乙虫腈(Ethiprole)、溴氰虫酰胺(Cyazypyr)、噻虫啉(Thiacloprid)、啶虫脒(Acetamiprid)、呋虫胺(Dinetofuran)、阿维菌素(Avermectin)、甲硫威(Methiocarb)、螺螨酯(spirodiclofen)、螺虫乙酯(Spirotetramat)；玉米杀真菌剂：种衣酯(Fenitropan)、福美双(Thiram)、丙硫菌唑(Prothioconazole)、戊唑醇(Tebuconazole)、肟菌酯(Trifloxystrobin)；稻除草剂：丁草胺(Butachlor)、敌稗(Propanil)、四唑嘧磺隆(Azimsulfuron)、苄嘧磺隆(Bensulfuron)、氰氟草酯(Cyhalofop)、杀草隆(Daimuron)、四唑酰草胺(Fentrazamide)、咪唑磺隆(Imazosulfuron)、苯噻草胺(Mefenacet)、嗪草酮(Oxaziclomefone)、吡嘧磺隆(Pyrazosulfuron)、稗草畏(Pyributicarb)、二氯喹啉酸(Quinclorac)、禾草丹(Thiobencarb)、茚草酮(Indanofan)、氟噻草胺(Flufenacet)、四唑酰草胺(Fentrazamide)、氯吡嘧磺隆(Halosulfuron)、嗪草酮(Oxaziclomefone)、双环磺草酮(Benzobicyclon)、环酯草醚(Pyriftalid)、五氟磺草胺(Penoxsulam)、双草醚(Bispyribac)、丙炔口恶草酮(Oxadiargyl)、乙氧磺隆(Ethoxysulfuron)、丙草胺(Pretilachlor)、甲基磺草酮(Mesotrione)、Tefuryltrione、口恶草酮(Oxadiazone)、口恶唑禾草灵(Fenoxaprop)、Pyrimisulfan；稻杀虫剂：地亚农(Diazinon)、杀螟硫磷(Fenitrothion)、仲丁威(Fenobucarb)、久效磷(Monocrotophos)、丙硫克百威(Benfuracarb)、噻嗪酮(Buprofezin)、呋虫胺(Dinotefuran)、氟虫腈(Fipronil)、吡虫啉(Imidacloprid)、异丙威(Isoprocarb)、噻虫啉(Thiacloprid)、环虫酰肼(Chromafenozide)、噻虫啉(Thiacloprid)、呋虫胺(Dinotefuran)、可尼丁(Clothianidin)、乙虫腈(Ethiprole)、氟虫双酰胺(Flubendiamide)、氯虫苯甲酰胺(Rynaxypyr)、溴氰菊酯(Deltamethrin)、啶虫脒(Acetamiprid)、噻虫嗪(Thiamethoxam)、溴氰虫酰胺(Cyazypyr)、艾克敌(spinosad)、Spinotoram、氨基阿维菌素苯甲酸盐(Emamectin-benzoate)、氯氰菊酯(Cypermethrin)、毒死蜱(Chlorpyriphos)、杀螟丹(Cartap)、对甲胺磷(Methamidophos)、醚菊酯(Etofenprox)、三唑磷(Triazophos)、4-[[(6-氯吡啶-3-基)甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮、克百威(Carbofuran)、丙硫克百威(Benfuracarb；稻杀真菌剂：甲基硫菌灵(Thiophanate-methyl)、嘧菌酯(Azoxystrobin)、环丙酰亚胺(Carpropamid)、敌瘟磷(Edifenphos)、嘧菌腙(Ferimzone)、异稻瘟净(Iprobenfos)、稻瘟灵(Isoprothiolane)、戊菌隆(Pencycuron)、烯丙苯噻唑(probenazole)、咯喹酮(Pyroquilon)、三环唑(Tricyclazole)、肟菌酯(Trifloxystrobin)、双氯氰菌胺(Diclocymet)、稻瘟酰胺(Fenoxanil)、硅氟唑(Simeconazole)、噻酰菌胺(Tiadinil)；棉花除草剂：敌草隆(Diuron)、伏草隆(Fluometuron)、MSMA、乙氧氟草醚(Oxyfluorfen)、扑草净(Prometryn)、氟乐灵(Trifluralin)、唑草酮(Carfentrazone)、烯草酮(Clethodim)、精吡氟禾草灵(Fluazifop-butyl)、草甘膦(Glyphosate)、氟草敏(Norflurazon)、二甲戊(Pendimethalin)、嘧草硫醚(Pyrithiobac-sodium)、三氟啶磺隆(Trifloxysulfuron)、吡喃草酮(Tepraloxydim)、草铵膦(Glufosinate)、丙炔氟草胺(Flumioxazin)、噻苯隆(Thidiazuron)；棉花杀虫剂：乙酰甲胺磷(Acephate)、涕灭威(Aldicarb)、毒死蜱(Chlorpyrifos)、氯氰菊酯(Cypermethrin)、溴氰菊酯(Deltamethrin)、马拉硫磷(Malathion)、久效磷(Monocrotophos)、阿维菌素(Abamectin)、啶虫脒(Acetamiprid)、甲胺基阿维菌素苯甲酸盐(Emamectin Benzoate)、吡虫啉(Imidacloprid)、二唑虫(Indoxacarb)、高三氟氯氰菊酯(Lambda-Cyhalothrin)、艾克敌(spinosad)、硫双威(Thiodicarb)、γ-氯氟氰菊酯、螺甲螨酯(spiromesifen)、啶虫丙醚(Pyridalyl)、氟啶虫酰胺(Flonicamid)、氟虫双酰胺(Flubendiamide)、杀铃脲(Triflumuron)、氯虫苯甲酰胺(Rynaxypyr)、Beta-Cyfluthrin、螺虫乙酯(Spirotetramat)、可尼丁(Clothianidin)、噻虫嗪(Thiamethoxam)、噻虫啉(Thiacloprid)、呋虫胺(Dinetofuran)、氟虫双酰胺(Flubendiamide)、溴氰虫酰胺(Cyazypyr)、艾克敌(spinosad)、Spinotoram、γ氯氟氰菊酯、4-[[(6-氯吡啶-3-基)甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮、硫双威(Thiodicarb)、阿维菌素(Avermectin)、氟啶虫酰胺(Flonicamid)、啶虫丙醚(Pyridalyl)、螺甲螨酯(spiromesifen)、氟啶虫胺腈(Sulfoxaflor)、丙溴磷(Profenophos)、三唑磷(Thriazophos)、硫丹(Endosulfan)；棉花杀真菌剂：土菌灵(Etridiazole)、甲霜灵(Metalaxyl)、五氯硝基苯(Quintozene)；大豆除草剂：甲草胺(Alachlor)、灭草松(Bentazone)、氟乐灵(Trifluralin)、氯嘧磺隆(Chlorimuron-Ethyl)、氯酯磺草胺(Cloransulam-Methyl)、口恶唑禾草灵(Fenoxaprop)、氟磺胺草醚(Fomesafen)、吡氟禾草灵(Fluazifop)、草甘膦(Glyphosate)、甲氧咪草烟(Imazamox)、咪唑喹啉酸(Imazaquin)、咪唑乙烟酸(Imazethapyr)、精异丙甲草胺(S-)Metolachlor)、嗪草酮(Metribuzin)、二甲戊(Pendimethalin)、吡喃草酮(Tepraloxydim)、草铵膦(Glufosinate)；大豆杀虫剂：高三氟氯氰菊酯(Lambda-Cyhalothrin)、灭多威(Methomyl)、对硫磷(Parathion)、硫双威(Thiocarb)、吡虫啉(Imidacloprid)、可尼丁(Clothianidin)、噻虫嗪(Thiamethoxam)、噻虫啉(Thiacloprid)、啶虫脒(Acetamiprid)、呋虫胺(Dinetofuran)、氟虫双酰胺(Flubendiamide)、氯虫苯甲酰胺(Rynaxypyr)、溴氰虫酰胺(Cyazypyr)、艾克敌(spinosad)、Spinotoram、氨基阿维菌素苯甲酸盐(Emamectin-benzoate)、氟虫腈(Fipronil)、乙虫腈(Ethiprole)、溴氰菊酯(Deltamethrin)、β-氟氯氰菊酯(β-Cyfluthrin)、γ和λ氯氟氰菊酯、4-[[(6-氯吡啶-3-基)甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮、螺虫乙酯(Spirotetramat)、Spinodiclofen、杀铃脲(Triflumuron)、氟啶虫酰胺(Flonicamid)、硫双威(Thiodicarb)、高效氟氯氰菊酯(Beta-Cyfluthrin)；大豆杀真菌剂：嘧菌酯(Azoxystrobin)、环丙唑醇(Cyproconazole)、氟环唑(Epoxiconazole)、粉唑醇(Flutriafol)、吡唑醚菌酯(Pyraclostrobin)、戊唑醇(Tebuconazole)、肟菌酯(Trifloxystrobin)、丙硫菌唑(Prothioconazole)、四氟醚唑(Tetraconazole)；甜菜除草剂：氯草敏(Chloridazon)、双苯胺灵(Desmedipham)、乙氧呋草黄(Ethofumesate)、甲双苯胺灵(Phenmedipham)、野麦畏(Triallate)、二氯吡啶酸(Clopyralid)、吡氟禾草灵(Fluazifop)、环草定(Lenacil)、苯嗪草酮(Metamitron)、氯甲喹啉酸(Quinmerac)、噻草酮(Cycloxydim)、氟胺磺隆(Triflusulfuron)、吡喃草酮(Tepraloxydim)、喹禾灵(Quizalofop)；甜菜杀虫剂：吡虫啉(Imidacloprid)、可尼丁(Clothianidin)、噻虫嗪(Thiamethoxam)、噻虫啉(Thiacloprid)、啶虫脒(Acetamiprid)、呋虫胺(Dinetofuran)、溴氰菊酯(Deltamethrin)、β-氟氯氰菊酯(β-Cyfluthrin)、γ/λ氯氟氰菊酯、4-[[(6-氯吡啶-3-基)甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮、七氟菊酯(Tefluthrin)、氯虫苯甲酰胺(Rynaxypyr)、Cyaxypyr、氟虫腈(Fipronil)、克百威(Carbofuran)；卡诺拉油菜除草剂：二氯吡啶酸(Clopyralid)、禾草灵酸(Diclofop)、吡氟禾草灵(Fluazifop)、草铵膦(Glufosinate)、草甘膦(Glyphosate)、吡唑草胺(Metazachlor)、氟乐灵(Trifluralin)、胺苯磺隆(EthaMetsulfuron)、氯甲喹啉酸(Quinmerac)、喹禾灵(Quizalofop)、烯草酮(Clethodim)、吡喃草酮(Tepraloxydim)；卡诺拉油菜杀真菌剂：嘧菌酯(Azoxystrobin)、多菌灵(Carbendazim)、咯菌腈(Fludioxonil)、异菌脲(Iprodione)、咪鲜胺(Prochloraz)、乙烯菌核利(Vinclozolin)；卡诺拉油菜杀虫剂：克百威(Carbofuran)、有机磷农药类、拟除虫菊酯类、噻虫啉(Thiacloprid)、溴氰菊酯(Deltamethrin)、吡虫啉(Imidacloprid)、可尼丁(Clothianidin)、噻虫嗪(Thiamethoxam)、啶虫脒(Acetamiprid)、呋虫胺(Dinetofuran)、β-氟氯氰菊酯(β-Cyfluthrin)、γ和λ氯氟氰菊酯、τ-氟胺氰菊酯(Fluvaleriate)、乙虫腈(Ethiprole)、艾克敌(spinosad)、Spinotoram、氟虫双酰胺(Flubendiamide)、氯虫苯甲酰胺(Rynaxypyr)、溴氰虫酰胺(Cyazypyr)、4-[[(6-氯吡啶-3-基)甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮。
“害虫”包括但是不限于昆虫、真菌、细菌、线虫、螨、蜱等。昆虫害虫包括选自鞘翅目(Coleoptera)、双翅目(Diptera)、膜翅目(Hymenoptera)、鳞翅目(Lepidoptera)、食毛目(Mallophaga)、同翅目(Homoptera)、半翅目(Hemiptera)、直翅目(Orthroptera)、缨翅目(Thysanoptera)、革翅目(Dermaptera)、等翅目(Isoptera)、虱目(Anoplura)、蚤目(Siphonaptera)、毛翅目(Trichoptera)等，特别地选自鞘翅目、鳞翅目和双翅目的昆虫。
鞘翅目包括肉食亚目(Adephaga)和多食亚目(Polyphaga)。肉食亚目包括步甲总科(Caraboidea)和豉甲总科(Gyrinoidea)、而多食亚目包括水龟甲总科(Hydrophiloidea)、隐翅甲总科(Staphylinoidea)、花萤总科(Cantharoidea)、郭公甲总科(Cleroidea)、叩甲总科(Elateroidea)、花甲总科(Dascilloidea)、泥甲总科(Dryopoidea)、丸甲总科(Byrrhoidea)、扁甲总科(Cucujoidea)、芫菁总科(Meloidea)、花蚤总科(Mordelloidea)、拟步甲总科(Tenebrionoidea)、长蠹总科(Bostrichoidea)、金龟总科(Scarabaeoidea)、天牛总科(Cerambycoidea)、叶甲总科(Chrysomeloidea)和象甲总科(Curculionoidea)。步甲总科包括虎甲科(Cicindelidae)、步甲科(Carabidae)和龙虱科(Dytiscidae)。豉甲总科包括豉甲科(Gyrinidae)。水龟甲总科包括水龟甲科(Hydrophilidae)。隐翅甲总科包括葬甲科(Silphidae)和隐翅甲科(Staphylinidae)。花萤总科包括花萤科(Cantharidae)和萤科(Lampyridae)。郭公甲总科包括郭公甲科(Cleridae)和皮蠹科(Dermestidae)。叩甲总科包括叩甲科(Elateridae)和吉丁甲科(Buprestidae)。扁甲总科包括瓢甲科(Coccinellidae)。芫菁总科包括芫箐科(Meloidae)。拟步甲总科包括拟步甲科(Tenebrionidae)。金龟总科包括黑蜣科(Passalidae)和金龟科(Scarabaeidae)。天牛总科包括天牛科(Cerambycidae)。叶甲总科包括叶甲科(Chrysomelidae)。象甲总科包括象甲科(Curculionidae)和小蠹科(Scolytidae)。
双翅目包括亚目长角亚目(Nematocera)、短角亚目(Brachycera)和环裂亚目(Cyclorrhapha)。长角亚目(Nematocera)包括大蚊科(Tipulidae)、毛蠓科(Psychodidae)、蚊科(Culicidae)、蠓科(Ceratopogonidae)、摇蚊科(Chironomidae)、蚋科(Simuliidae)、毛蚊科(Bibionidae)和瘿蚊科(Cecidomyiidae)。短角亚目包括水虻科(Stratiomyidae)、虻科(Tabanidae)、剑虻科((Therevidae)、食虫虻科(Asilidae)、拟蜂虻科(Mydidae)、蜂虻科(Bombyliidae)和长足虻科(Dolichopodidae)。环裂亚目(Cyclorrhapha)包括无缝组(Aschiza)和有缝组。无缝组包括科蚤蝇科(Phoridae)、食蚜蝇科(Syrphidae)和眼蝇科(Conopidae)。有缝组包括无瓣类(Acalyptratae)和有瓣类(Calyptratae)。无瓣类包括斑蝇科(Otitidae)、实蝇科(Tephritidae)、潜蝇科(Agromyzidae)和果蝇科(Drosophilidae)。有瓣类包括科虱蝇科(Hippoboscidae)、狂蝇科(Oestridae)、寄蝇科(Tachinidae)、花蝇科(Anthomyiidae)、蝇科(Muscidae)、丽蝇科(Calliphoridae)和麻蝇科(Sarcophagidae)。
鳞翅目包括凤蝶科(Papilionidae)、粉蝶科(Pieridae)、灰蝶科(Lycaenidae)、蛱蝶科(Nymphalidae)、斑蝶科(Danaidae)、眼蝶科(Satyridae)、弄蝶科(Hesperiidae)、天蛾科(Sphingidae)、大蚕蛾科(Saturniidae)、尺蛾科(Geometridae)、灯蛾科(Arctiidae)、夜蛾科(Noctuidae)、毒蛾科(Lymantriidae)、透翅蛾科(Sesiidae)和谷蛾科(Tineidaee)。
线虫包括寄生性线虫如根结线虫、胞囊线虫和根腐线虫，包括胞囊线虫属(Heterodera)某些物种、根结线虫属(Meloidogyne)某些物种和球胞囊属(Globodera)某些物种；特别地是胞囊线虫成员，包括但不限于Heterodera glycines(大豆胞囊线虫)；Heterodera schachtii(甜菜胞囊线虫)；Heterodera avenae(谷类胞囊线虫)；和马铃薯金线虫(Globodera rostochiensis)和马铃薯白线虫(Globodera pailida)(马铃薯胞囊线虫)。根腐线虫包括短体属(Pratylenchus)某些物种，例如穿刺根腐线虫(Pratylenchuspenetrans)。
对于主要作物的本发明昆虫害虫包括：玉米：玉米螟(Ostrinia nubilalis)、欧洲玉米钻心虫(European corn borer)；小地老虎(Agrotis ipsilon)、黑地蚕(blackcutworm)；谷实夜蛾(Helicoverpa zea)、美洲棉铃虫(corn earworm)；草地夜蛾(Spodoptera frugiperda)、秋夜蛾(fall armyworm)；西南玉米杆草螟(Diatraeagrandiosella)、西南玉米钻心虫(southwestern corn borer)；南美玉米苗斑螟(Elasmopalpus lignosellus)、小玉米茎钻心虫(lesser cornstalk borer)；小蔗螟(Diatraea saccharalis)、甘蔗钻心虫(surgarcane borer)；玉米根叶甲(Diabroticavirgifera)、西方玉米根虫(western corn rootworm)；长角叶甲barberi亚种(Diabroticalongicornis barberi)、北方玉米根虫(northern corn rootworm)；黄瓜十一星叶甲食根亚种(Diabrotica undecimpunctata howardi)、南方玉米根虫(southern cornrootworm)；纹路叩甲属(Melanotus spp.)、铁线虫(wireworms)；圆头犀金龟(Cyclocephala borealis)、圆头独角仙(northern masked chafer(蛴螬))；圆头无斑犀金龟(Cyclocephala immaculata)、南方圆头犀金龟(southern masked chafer(蛴螬))；日本金龟(Popillia japonica)、日本甲虫(Japanese beetle)；玉米铜色跳甲(Chaetocnemapulicaria)、玉米跳甲(corn flea beetle)；玉米谷喙甲(Sphenophorus maidis)、玉米谷象(maize billbug)；玉米缢管蚜(Rhopalosiphum maidis)、玉米蚜(corn leaf aphid)；玉米根蚜(Anuraphis maidiradicis，corn root aphid)；美洲谷长蝽(Blissus leucopterusleucopterus)、麦虱(chinch bug)；赤腿黑蝗(Melanoplus femurrubrum)、赤腿蚱蜢(redlegged grasshopper)；血黑蝗(Melanoplus sanguinipes)、迁徙蚱蜢(migratorygrasshopper)；玉米种蝇(Hylemya platura)、玉米种蛆(seedcorn maggot)；美洲黍潜叶蝇(Agromyza parvicornis)、玉米斑潜叶蝇(corn blot leafminer)；玉米黄呆蓟马(Anaphothrips obscrurus)、草蓟马(grass thrips)；窃叶蚁(Solenopsis milesta，thiefant)；二斑叶螨(Tetranychus urticae)、二点叶螨(twospotted spider mite)；高粱：玉米禾螟(Chilo partellus)、高粱钻心虫(sorghum borer)；草地夜蛾，秋夜蛾；谷实夜蛾，美洲棉铃虫；南美玉米苗斑螟，小玉米茎钻心虫；粒肤地老虎(Feltia subterranea，granulatecutworm)；Phyllophaga crinita，蛴螬；伪金针虫属(Eleodes)、Conoderus和Aeolus属某些物种，铁线虫；黑甲负泥虫(Oulema melanopus)、(谷类植物跳甲)；玉米铜色跳甲(Chaetocnema pulicaria)、玉米跳甲(corn flea beetle)；玉米谷喙甲(Sphenophorusmaidis)、玉米谷象(maize billbug)；玉米缢管蚜(Rhopalosiphum maidis)、玉米蚜(cornleaf aphid)；蔗黄伪毛蚜(Sipha flava)、甘蔗黄蚜(yellow sugarcane aphid)；美洲谷长蝽(Blissus leucopterus leucopterus)、麦虱(chinch bug)；高粱瘿蚊(Contariniasorghicola)、高粱蠓(sorghum midge)；朱砂叶螨(Tetranychus cinnabarinus)、棉红蜘蛛(carmine spider mite)；二斑叶螨(Tetranychus urticae)、二点叶螨(twospottedspider mite)；小麦：美洲一星粘虫(Pseudaletia unipunctata)、行军虫(army worm)；草地夜蛾(Spodoptera frugiperda)、秋夜蛾(fall armyworm)；南美玉米苗斑螟(Elasmopalpus lignosellus)、小玉米茎钻心虫(lesser cornstalk borer)；Agrotisorthogonia，西部切根虫(western cutworm；南美玉米苗斑螟(Elasmopalpuslignosellus)、小玉米茎钻心虫(lesser cornstalk borer)；黑甲负泥虫，禾谷跳甲；三叶草叶象(Hypera punctata)、车轴草叶象甲(clover leaf weevil)；黄瓜十一星叶甲食根亚种，南方玉米根虫；俄罗斯麦蚜(Russian wheat aphid)；麦二叉蚜(Schizaphisgraminum)、绿虫(greenbug)；麦长管蚜(Macrosiphum avenae)、英国禾谷蚜(Englishgrain aphid)；赤腿黑蝗，赤腿蚱蜢；殊种黑蝗，殊种蚱蜢；血黑蝗，迁徙蚱蜢；小麦瘿蚊(Mayetiola destructor)、黑森瘿蚊(hessian fly)；麦红吸浆虫(Sitodiplosismosellana)、麦蠓(wheat midge)；美洲麦杆蝇(Meromyza americana，wheat stemmaggot)；冬作种蝇(Hylemya coarctata，wheat bulb fly)；烟草蓟马(Frankliniellafusca)、烟蓟马(tobacco thrips)；茎锯蜂(Cephus cinctus)、麦茎蜂(wheat stemsawfly)；郁金香瘤瘿螨(Aceria tulipae)、麦瘿螨(wheat curl mite)；向日葵：Suleimahelianthana，sunflower bud moth；向日葵斑螟(Homoeosoma electellum)、美洲向日葵螟(sunflower moth)；向日葵叶甲(zygogramma exclamationis，sunflower beetle)；胡萝卜金龟(Bothyrus gibbosus，carrot beetle)；向日葵籽瘿蚊(Neolasiopteramurtfeldtiana，sunflower seed midge)；棉属植物：烟蚜夜蛾(Heliothis virescens)、棉卷叶蛾(cotton budworm)；谷实夜蛾(Helicoverpa zea)、美洲棉铃虫(cotton bollworm)；甜菜夜蛾(Spodoptera exigua)、甜菜粘虫(beet armyworm)；红铃麦蛾(Pectinophoragossypiella)、棉红铃虫(pink bollworm)；墨西哥棉铃象(Anthonomus grandis)、棉铃象甲(boll weevil)；棉蚜(Aphis gossypii，cotton aphid)；棉跳盲蝽(Pseudatomoscelisseriatus，cotton fleahopper)；结翅粉虱(Trialeurodes abutilonea，bandedwingedwhitefly)；牧草盲蝽(Lygus lineolaris，tarnished plant bug)；赤腿黑蝗，赤腿蚱蜢；殊种黑蝗，殊种蚱蜢；棉蓟马(Thrips tabaci)、洋葱蓟马(onion thrips)；烟草蓟马，草蓟马；朱砂叶螨，棉红蜘蛛；二斑叶螨，二点叶螨；稻:小蔗螟，甘蔗钻心虫；草地夜蛾，秋夜蛾；谷实夜蛾，美洲棉铃虫；葡萄肖叶甲(Colaspis brunnea，grape colaspis)；稻水象甲(Lissorhoptrus oryzophilus，rice water weevil)；米象(Sitophilus oryzae，riceweevil)；黑尾叶蝉(Nephotettix nigropictus)、稻叶蝉(rice leafhopper)；美洲谷长蝽，麦虱；喜绿蝽(Acrosternum hilare，green stink bug)；大豆：大豆夜蛾(Pseudoplusiaincludens)、大豆尺蠖(soybean looper)；梨豆夜蛾(Anticarsia gemmatalis)、梨豆毛虫(velvetbean caterpillar)；苜蓿绿夜蛾(Plathypena scabra，green cloverworm)；玉米螟，欧洲玉米钻心虫；小地老虎，黑地蚕；甜菜夜蛾，甜菜粘虫；烟蚜夜蛾，棉卷叶蛾；谷实夜蛾，美洲棉铃虫；墨西哥豆瓢虫(Epilachna varivestis，Mexican bean beetle)；桃蚜(Myzus persicae，green peach aphid)；蚕豆微叶蝉(Empoasca fabae)、马铃薯叶蝉(potato leafhopper)；喜绿蝽(Acrosternum hilare)、绿臭蝽(green stink bug)；赤腿黑蝗，赤腿蚱蜢；殊种黑蝗，殊种蚱蜢；玉米种蝇，玉米种蛆；Sericothrips variabilis、大豆蓟马(soybean thrips)；棉蓟马，洋葱蓟马；土耳其斯坦叶螨(Tetranychus turkestani，strawberry spider mite)；二斑叶螨，二点叶螨；大麦：玉米螟，欧洲玉米钻心虫；小地老虎，黑地蚕；麦二叉蚜，绿虫；美洲谷长蝽，麦虱；喜绿蝽，绿臭蝽；褐臭椿(Euschistusservus，brown stink bug)；玉米种蝇，玉米种蛆；小麦瘿蚊，黑森瘿蚊；麦岩螨(Petrobialatens，brown wheat mite)；油籽油菜(Oil Seed Rape)：甘蓝短棒蚜(Brevicorynebrassicae)、甘蓝蚜(cabbage aphid)；十字花科跳甲(Phyllotreta cruciferae，Fleabeetle)；蓓带夜蛾(Mamestra configurata、Bertha armyworm)；小菜蛾(Plutellaxylostella，Diamond-back moth)；地种蝇属某些物种(Delia ssp.)、根蛆(Rootmaggots)。
用于增加植物产量的方法
提供了用于增加植物产量的方法。所述方法包括提供植物或植物细胞，其表达编码本文中所公开的杀虫多肽序列的多核苷酸，和在被害虫侵袭的田间生长该植物或其种子，其中所述多肽对所述害虫具有杀虫活性。在一些实施方案中，所述多肽具有针对鳞翅目的、鞘翅目的、双翅目的、半翅目的或线虫类的害虫的杀虫活性并且所述的田地用鳞翅目的、半翅目的、鞘翅目的、双翅目的或线虫类的害虫侵袭。
如本文中定义，植物的“产量”指由植物产生的生物量的质量和/或数量。“生物量”意指任何所度量的植物产物。生物量产生的增加是所度量植物产物的产量方面的任意改善。增加植物产量具有几项商业应用。例如，增加植物叶生物量可以增加用于人或动物消费的叶菜类的产量。另外，增加叶生物量可以用来增加植物衍生的药用或工业产物的生产。产量的增加可以包含任何统计显著的增加，包括但不限于与不表达杀虫序列的植物相比，产量增加至少1％、增加至少3％、增加至少5％、增加至少10％、增加至少20％、增加至少30％、至少50％、至少70％、至少100％或更多。
在具体的方法中，植物产量因表达本文中所公开杀虫蛋白的植物的改善的害虫抗性而增加。杀虫蛋白的表达导致害虫侵袭或采食植物的能力降低，因而，改善植物产量。
以下实施例以说明方式且不以限制方式提供。
实施例1.来自苏云金芽孢杆菌的与杀虫基因具有同源性的新杀虫基因的发现
使用如下步骤从表1中列出的细菌菌株中鉴定新杀虫基因：
·从包含通常携带Δ-内毒素基因的质粒的菌株中制备染色体外DNA
·机械剪切染色体外DNA以产生大小分布的片段
·克隆约2Kb至约10Kb的染色体外DNA片段
·生长出染色体外DNA的约1500个克隆
·使用克隆载体特异性引物，对这1500个克隆进行部分测序(末端读取)
·通过MiDAS方法(如通过引用方式完整并入本文的美国专利公开号20040014091中所述)，通过同源性分析鉴定推定的毒素基因
·含有推定的目的毒素基因的片段的克隆的序列整理(步行法)
表1
1与axmi201配对
2与axmi200配对
3与axmi214配对
4与axmi213配对
5基于检查ATX24031的序列，鉴定了两个重叠的可读框(ORFs)，每一个可读框都具有与内毒素样基因的同源性。检查了这些ORF及它们编码的蛋白质后，显而易见，这两个ORFs可能起源于单一的ORF，所述单一的ORF在从第一个ORF开始的核苷酸224至309区域中经历了单核苷插入(或者产生了单核苷酸移码的更大插入)。将这些orf在本文中称为ATX24031_contig4_orf1(SEQ ID NO:63)和ATX424031_contig4_orf2(SEQ ID NO:64)。全长序列在SEQ ID NO:65中示出。通过“固定”插入来产生单一的ORF，可以装配与内毒素完全同源的复合ORF。然而应当理解，可以创造多种解决方案来产生此种ORF，并且这些解决方案在ORFs之间重叠的区域方面不同，本文提供了一种解决方案，称为axmi196(SEQ ID NO:7)。
6在紧接axmi211的上游鉴定了p19/CryBP1样基因。这种基因的核苷酸序列在SEQID NO:66中示出，并在SEQ ID NO:67中示出了氨基酸序列。
6在紧接axmi212的上游鉴定了p19样基因。这种基因的核苷酸序列在SEQ ID NO:68中示出，并在SEQ ID NO:69中示出了氨基酸序列。
实施例2.在芽孢杆菌属中表达
通过PCR从pAX980扩增本文中所公开的杀虫基因并且通过本领域熟知的方法，将PCR产物克隆到芽孢杆菌表达载体pAX916或另一个合适载体中。含有携带axmi基因的载体的所得芽孢杆菌菌株在常规生长培养基上培养，如CYS培养基(10g/l细菌用酪蛋白胨；3g/l酵母提取物；6g/l KH2PO4；14g/l K2HPO4；0.5mM MgSO4；0.05mM MnCl2；0.05mM FeSO4)，直至通过显微镜检表明芽孢形成明显。制备样品并在生物测定法中测试活性。
实施例3.Axmi-191和Axmi-192的杀虫活性
基因表达和纯化
编码Axmi-191和Axmi-192的毒素结构域的多个DNA区域独立地克隆到大肠杆菌表达载体pMAL-C4x中于编码麦芽糖结合蛋白(MBP)的malE基因之后。这些符合读码框的融合导致大肠杆菌中的MBP-Axmi融合蛋白表达。
为了在大肠杆菌中表达，BL21*DE3以各个质粒转化。将单菌落接种到补充有羧苄西林和葡萄糖的LB中并且在37℃培育过夜。次日，新鲜培养基用1％过夜培养物接种并且在37℃生长至对数期。随后，培养物用0.3mM IPTG在20℃诱导过夜。将每份细胞沉淀悬浮于20mM Tris-Cl缓冲液，pH 7.4+200mM NaCl+1mM DTT+蛋白酶抑制剂中并作超声处理。通过SDS-PAGE分析证实了融合蛋白的表达。
使无细胞的总提取物经过与FPLC连接的直链淀粉柱以亲和纯化MBP-axmi融合蛋白。结合的融合蛋白用10mM麦芽糖溶液从树脂洗脱。纯化的融合蛋白随后以因子Xa切割以从Axmi蛋白去除氨基端MBP标签。通过SDS-PAGE确定蛋白质的切割和溶解性。
昆虫生物测定法
在带有适宜对照的昆虫测定法中测试切割的蛋白质。对平板读取5天，显示Axmi-191和Axmi-192具有针对小菜蛾(Diamondback moth)的活性。Axmi191显示出引起矮化，且Axmi192显示出引起严重矮化和100％的死亡率。
实施例4.合成序列的构建
在本发明的一个方面，产生了合成的毒素序列。这些合成的序列相对于亲代毒素序列具有改变的DNA序列，并且编码这样的蛋白质，该蛋白质与其相对应的亲代毒素蛋白相符，但是缺少在许多Δ-内毒素蛋白中存在的C末端“结晶结构域”。
在本发明的另一个方面，如此设计改变形式的合成基因，从而将所得的肽靶向植物细胞器，如内质网或质外体。已知导致融合蛋白靶向植物细胞器的肽序列是本领域已知的。例如，本领域已知来自白羽扇豆(Lupinus albus)的酸性磷酸酶基因(Genebank ID GI:14276838；Miller等人(2001)Plant Physiology 127:594-606)的N端区导致异源蛋白靶向内质网。如果所得的融合蛋白还在C末端含有包含肽N-末端-赖氨酸-天冬氨酸-谷氨酸-亮氨酸(即，“KDEL”基序，SEQ ID NO:70)的内质网停留序列，则该融合蛋白会被靶向内质网。如果融合蛋白在C末端处缺少内质网靶向序列，则蛋白质将会被靶向内质网，但最终会隔绝在质外体中。
实施例5.杀虫活性的测定法
常常以众多方式评估杀虫蛋白作为杀虫剂对害虫发挥作用的能力。本领域熟知的一种方式是进行饲喂测定法。在这种饲喂测定法中，使害虫暴露于含有待测试化合物的样品或对照样品。这常常通过将待测试物质或该物质的合适稀释物放置到害虫会摄取的物质(如人工食物)上进行。待测试的物质可以由液体、固体或浆液组成。待测试的物质可以置于表面上并且随后允许干燥。备选地，待测试的物质可以与熔融的人工食物混合，随后分配至测定箱中。测定箱可以是例如杯、碟或微量滴定板的孔。
用于吸吮性害虫(例如蚜虫)的测定法可以涉及试验材料与昆虫通过隔离物(理想地是可以被吸吮昆虫的吸口部分穿透的部分)分隔以允许摄取试验材料。该试验材料常常与饲喂刺激物如蔗糖混合以促进试验化合物的摄取。
其他类型的测定法可以包括微量注射试验材料至害虫的口或肠，以及产生转基因植物，随后测试害虫以该转基因植物为食后的能力。植物测试法可以涉及分离通常消费的植物部分，例如，附着在叶子上的小笼状物，或在含有昆虫的小笼状物中分离完整植株。
测定害虫的其他方法和手段是本领域已知的，并且可以例如在Robertson,J.L.和H.K.Preisler(1992)Pesticide bioassays with arthropods,CRC,Boca Raton,FL中找到。备选地，测定法通常在期刊“Arthropod Management Tests”和“Journal of EconomicEntomology”中或通过美国昆虫学学会(Entomological Society of America(ESA))”成员讨论来描述。
实施例6.pMal融合蛋白的表达
为了在大肠杆菌中表达，将本发明的选定基因克隆入pMal表达载体(New EnglandBiolabs)中，以使在大肠杆菌中表达的该蛋白质具有N端融合至麦芽糖结合蛋白(MBP)。将编码Axmi207的截短变体的核苷酸序列(对应于SEQ ID NO:47的位置15至647)用于生物活性的测试。截短的变体Axmi207序列在SEQ ID NO:62中示出。对于Axmi196、Axmi204和Axmi209，使用全长天然序列。
随后通过本领域已知的亲和层析纯化这些融合蛋白。然后用本领域已知的蛋白酶切割纯化的蛋白质，以将MBP从本发明的蛋白质中分离。然后将所得的蛋白质针对选定的害虫在生物测定法中测试。结果显示于表2中。
表2.
DBM–小菜蛾
CPB–科罗拉多马铃薯叶甲虫(Colorado potato beetle)
SWCB–西南玉米钻心虫
VBC–梨豆毛虫(Velvetbean caterpillar)
ECB–欧洲玉米钻心虫
Hz–谷实夜蛾
FAW–秋夜蛾
SCB–甘蔗螟虫(Sugarcane borer)
SCN–大豆孢囊线虫(Soybean cyst nematode)
实施例7.用于植物表达的本发明毒素基因的载体
本发明基因的每个编码区独立地与用于植物中表达的适宜启动子序列和终止子序列连接。此类序列是本领域熟知的，并且可以包括用于单子叶植物中表达的稻肌动蛋白启动子或玉米遍在蛋白启动子、用于双子叶植物中表达的拟南芥UBQ3启动子或CaMV 35S启动子和nos或PinII终止子。用于产生并证实启动子–基因–终止子构建体的技术也是本领域熟知的。
实施例8.本发明基因通过农杆菌介导的转化法转化入植物细胞中
在授粉后8-12日收获穗。从穗中分离胚，并且将大小0.8-1.5mm的那些胚用于转化。将胚以小盾片侧朝上平铺在合适的孵育培养基上，并且在25℃于黑暗下孵育过夜。然而，本身并不必需要孵育胚过夜。使胚与含有用于Ti质粒介导转移的适宜载体的农杆菌菌株接触5-10分钟，并且随后铺在共培育培养基上3天(25℃于黑暗下)。在共培育后，将外植体转移至恢复期培养基5天(在25℃于黑暗下)。取决于所用具体选择的性质和特征，在选择培养基中孵育外植体持续直至8周。在选择期之后，将所得的愈伤组织转移至胚成熟培养基，直至观察到成熟体细胞胚的形成。所得的成熟体细胞胚随后置于低光照下，并且如本领域已知那样启动再生的过程。允许所得的苗在生根培养基上生根，并且将所得的植物转移至苗圃钵并作为转基因植物繁殖。
实施例9.用本发明毒素基因转化玉米细胞
在授粉后8-12日收获玉米穗。从穗中分离胚，并且将大小0.8-1.5mm的那些胚用于转化。胚以小盾片侧朝上平铺在合适的孵育培养基如DN62A5S培养基(3.98g/L N6盐；1mL/L(1000x母液)N6维生素；800mg/L L-天冬酰胺；100mg/L肌醇；1.4g/L L-脯氨酸；100mg/L酪蛋白氨基酸；50g/L蔗糖；1mL/L(1mg/mL母液)2,4-D)上，并且在25℃于黑暗下孵育过夜。
将所得的外植体转移至筛板(mesh squares)(每个平板30-40个网孔)，转移到渗透培养基上30-45分钟，随后转移至照射平板(beaming plate)(见，例如，PCT公开号WO/0138514和美国专利号5,240,842)。
使用气溶胶束加速器，使用基本上如PCT公开号WO/0138514中所述的条件，将设计旨在植物细胞中表达本发明基因的DNA构建体加速至植物组织中。在照射后，将胚在渗透培养基上孵育30分钟，随后置于孵育培养基上在25℃黑暗中过夜。为避免过分损伤照射过的外植体，将它们在转移至恢复培养基之前孵育至少24小时。胚随后铺展在恢复期培养基上，于25℃黑暗下5日，随后转移至选择培养基。取决于所用具体选择的性质和特征，在选择培养基中孵育外植体持续直至8周。在选择期之后，将所得的愈伤组织转移至胚成熟培养基，直至观察到成熟体细胞胚的形成。所得的成熟体细胞胚随后置于低光照下，并且通过本领域已知的方法启动再生的过程。允许所得的苗在生根培养基上生根，并且将所得的植物转移至苗圃钵并作为转基因植物繁殖。
材料
DN62A5S培养基
用1N KOH/1N KCl调节溶液的pH至pH 5.8，添加Gelrite(Sigma)至3g/L，并且高压灭菌。在冷却至50℃后，添加2ml/L的5mg/ml硝酸银母液(植物技术实验室)。配方产生约20个平板。
本说明书中提到的全部出版物及专利申请说明了本发明所属领域的技术人员的水平。全部出版物及专利申请通过引用的方式以相同程度并入本文，如同专门且个别地说明通过引用方式并入每份单独的出版物或专利申请。
虽然为了清楚理解的目的已经通过阐述和实例的方式某种程度上详细地描述了上述发明，但是显然可以在所附权利要求的范围内进行某些改变和变化。
序列表
<110> 阿森尼克斯公司
<120> 杀虫基因的AXMI-192家族和使用它们的方法
<130> APA067 US01
<150> 61/230,659
<151> 2009-12-31
<160> 70
<170> 用于Windows版本4.0的FastSEQ
<210> 1
<211> 1922
<212> DNA
<213> 苏云金芽孢杆菌（Bacillus thuringiensis）
<400> 1
ttggaaatta aaattggaag cggaggaaca tatatgaatc catataacaa cgaaagctat 60
gaaattattg atttaaatac ttcaccttat ccttctaaca gaaataattc taggtatcct 120
tatgcaaatg cttgcggttt tccagaaaac gtagattgga cagcaggcgc aagtgcaatg 180
ataattgtag ctggtacttt attaagtgct ataggttcag gaggagtagg tatagttgct 240
gcaggtatta tatcagttgg tacactattc cctttttttt ggcctcaaga taagcctact 300
gcacaagtat ggaaagattt tattaaacaa ggggatacta taactaataa aacaatatca 360
gcagccgtag aatctctagt actcgcagaa ttaaatggtt taaaatctat acttgatgtt 420
tatactgatg ctttagaact ttggaaaaaa gataaaaata atatagtcaa tagagacaat 480
gtaaaaagta tttttacaaa tttacatcta caatttgtag ccgctatgcc aaaatttgca 540
acaaatggtt atgaagtaat attattatct acttacacag cagctgcact tcttcatatt 600
acttttttac atgaagctct tcaatatgca aatgaatgga atttagctcg aagtgaagga 660
accttctatc gtggacaatt aattcaagca atagaaaact acattaatta ttgtgaaaaa 720
tggtatcgtg aaggtttaga gatacttaaa aattctactt gggatatata tgctgcgtat 780
caaaatgaat acactctaag tatattaaat gttatttcaa tttttccaag atttgatata 840
cgtaatttcc ctacaaatat agcaactcga ttagaatcta cacaaaaact ttatacaaca 900
acaccaaata tgaaagcatt aaaaacaaat aactcaattg attatataaa agataaactt 960
atacctcctt tagatttatt taaaaaatta aaaagtttaa ctttttatac atttttagat 1020
agcaataacc aatatgatca tttacaaggt attgtaaata atagttatta tactaatatt 1080
tccactaaca aaatcttttc ttctggaact accgaaggta gttcatatca actaggtttg 1140
gcttctgatc aagttattta ctacactgac atcttccatc atctaaatca aagtaatttt 1200
aaggatggtt cccttggaat taaaataatt aattttaata ttataaataa atataatgag 1260
gtttctcaaa aatcttatga ttctaatgca acaagtaatc taatactaga agttatatta 1320
ccttttctaa aaacaactga gaaagattat aaatatattt tatcttatat tacaataact 1380
ccacagcaga tagtaggatg tctaagtcct agttatatat atggatttat ttggacacat 1440
agtagtgtta atcttaacaa tactattcat tatacaaata aaaataattt ttctcaaatt 1500
acacaaattt ctgcagtaaa agcatatctg aaaaaagatc gagtttcagt tatagaagga 1560
ccaggtcata caggcggaga tttagttaaa tttacacaat gggatgattc aatttcaact 1620
cattatcaat ttactagcag tggtgaatat aaaatacgtg tccgatatgc ttctactgct 1680
caagttaatc aaaccagcgg acttagtatg acgatatacc ataaaggaaa tcctacagaa 1740
acatgggatt taaacataaa taacaaatca gatacaatac ttaatttaaa tgaaccaaaa 1800
tacaatcatt ttcaatacac agaatttcca aataaaactc ttataataaa taaagaccca 1860
aattctccat acttagaact aagaatagac ttaagctata aaggaaatac tgcaacaact 1920
ct 1922
<210> 2
<211> 1518
<212> DNA
<213> 苏云金芽孢杆菌
<400> 2
atgaatggaa atggaaaaca tgataactgg aatcacaatc aacaaatatc aaatgtccaa 60
atgaaccaca atcatggtag atcatatgat tgcagttgtc aacaaaatca gtatgggtat 120
gagcaacaaa aacagcagta tgaacagaat aacagccaat atatgcagaa taatctggga 180
aacgaaaata ggaatggatt gtatccttat caagaaaatc aatatgaaca aaataagaat 240
tattatgcat caaacaattt aacatataat cagtctgatt tgtataattc taatcctcaa 300
aatatgtata aacatcaaac atattctaat gatttttatt gttctcctag ctatacagca 360
ggtgaaaata atatattaga tctattaggt acagaaagta aacaattcca aaaaatttca 420
aatataaata ctaaagattt acatcgaagt ataactgcga gcaatactca aattggttat 480
caaattgata ctcgtgttcc aggaccatgt aaaggtgtag attatcaaaa cacagtaacc 540
tatgaacaaa attcaatagg tggcgattcc caatacttga ttttttataa aacggattat 600
actgatgcat ttattattgc gaatagagca aatggtcgag ttttagaagt aatacctagt 660
tcagttaatg gttttgtaac aatttctaat atgtttactt ataatcaaaa tcaacttttt 720
attcgtacta aaatatcaaa taatgataat tcagatgatg ttccattttc tttaacaaca 780
gaaaacaatc aaacattaaa catatgccat catgaatttc aatataatac taaaattaca 840
gctcttgata atgcatatcg tttggatgat aaggttttat ttaaaccaac tagagataaa 900
atcaacatat catttccaaa tatggtagtg aatgcgaagg agaaattacc agaacccgag 960
gaattaacaa atatggataa gaatactctt tttataccga aagtgattat aagtaaaacg 1020
ttaattccag gtataattgt aaacgatgta actttattaa aggagcaaca aatagcaaaa 1080
agtccatatt atgtattaga atatgttcaa tcttgggaag aagtgtataa tgaaatagta 1140
cctgcttata gaccttcgta tacttggact tcaacagatg gaattagaca cgttaatcta 1200
ttagatataa agaatactat aaatatatca ataggtggaa ctagtcaagg ctggggatta 1260
agatttagtg ataaatcaga tctttttaaa aacataatta catcagcatt cattataaaa 1320
tcaacacaag ctccagatat gggattcagt gagaatgata tagatcagta ctatggtaag 1380
aatattgaca gtagagttaa aatatatata aaaacccata atttaatatt aagacgttta 1440
gatcaattga acaattcaat agctacatgg acaatatttg agaatacaaa acctgttata 1500
agaacgtttc caattagt 1518
<210> 3
<211> 2337
<212> DNA
<213> 苏云金芽孢杆菌
<400> 3
atgaaggata aaaaatactg gaaatacgag ggaggaacca aaatgaatcc ttatcagaat 60
aagaatgaat atgaaatagt aaataatccg caaaattata atactgtttc caatagatat 120
ccttacacga atgatccaaa tgttgcaata caaaatacga attataaaga ttggatgaat 180
gggtatgaag aaattaatcc ttcttcaata tcgttaattt tagcttcaat aggaattctt 240
aatcaagcaa ttgctttaac tggagtatta ggtaagacac cagaaattat taacatagta 300
caagaaatgg tgggattaat tagcgggagt acaggcaatg atttattagt acatacagaa 360
caacttattc aacaaacttt agcacaacag tatagaaacg cagcaaccgg agcggtgaat 420
gctatatcta aatcatacaa tgattatttg atgtttttta ggcaatggga acgtaataga 480
acttctcaaa atggactaca agtagagagt gcttttaata ctgttaatac tttatgtctt 540
cgtactttaa ctcctcagga agcactttct cgcagaggat ttgaaactct tttattacca 600
aactatgcac tagcggcaaa tttccatttg ttattattaa gagatgctgt tctttataga 660
actcagtggt tacctaattt tatttcaact acaaatgcga atattgaaat attggaaagg 720
tccataaatc aatatcgtaa tcattgtaat cattggtaca atgacggctt aaatagattt 780
gcacgtacat cttttgatga ttgggttcgg tttaatgctt atcgtagaga tatgacgtta 840
tcggtattag attttgttac agtatttcca acttataatc ctataaactt tccaacacca 900
acaaatgttg aattgactag aatcgtttat accgatccaa taagtccacc tagaggatat 960
gcaagaactg gctcacctag ttttcgtcaa atggaagatc taattatttc cggtagccct 1020
agtttcttga atcaattaag tatatttaca acttattatc atgatcctcg taatgtaaat 1080
agagactttt gggccgggaa tcggaattat ttaagcaatg ggacttctcg acagtctgga 1140
gctaccacac cttggcgaac taatatacct atgcaaaaca ttgatatttt cagagtaaat 1200
ctaactactc atgacattga tgatatatca cgaagttatg gaggagttca tagatctgat 1260
ttcattggtg taaatacaat aaataatcaa agaacaacat tgttctatca ccaaaatgtg 1320
gatacttccc gttttctaat aaggaatgaa acagtatttt taccagggga ttccggctta 1380
gcaccaaatg aacgtaatta tactcacagg ttatttcaag tgatgaccac atatcgtact 1440
aacccgaatg ctcgtagggc agctttttta catgcatgga cgcatagaag tttaagacgt 1500
agaaatggat ttaggacgga tcagattatg caaatacctg ctgtgaagag cataagtaat 1560
ggtggtgatc gtgcagtcat atcctatact ggagaaaata tgatgaaatt agataactta 1620
actgcaagtt tatcctataa attaacagcg gaggattccg aagcatcgaa tacacgtttt 1680
atagtgcgta ttcgttatgc tagtatgaac aataatagat tgaatcttat tttaaatggt 1740
actcagatag catcgctgaa tgtggaaggt acaatgcaaa atggcggatc attaacaaat 1800
cttcaatctg aaaattttaa atatgctaca ttttcaggta atttcaagat gggttctcag 1860
tctatagtag gtatttttaa agagatatct aatgcagact ttattttaga taaaattgaa 1920
ttgattccaa ttcattttat gccattatta gaacaaaaac aaagctacaa caattacgac 1980
caaaacatgg atactacata tcaaccaaac tatgacactt ataatcaaaa tgccaatggt 2040
atgtatgacg atacatacta tccaaataat aatgatagtt ataatcaaaa taataccgat 2100
atgtatgatt caggctacaa taacaaccaa aatactaact ataattatga tcaagaatat 2160
aatacttaca atcaaaatat ggaaaatacg tatgaccaat cgtatgaaaa ttacaatcca 2220
gaaaccaaca attacaacca ataccctaat gatatgtaca atcaagagta tactaacgac 2280
tacaaccaaa actccggctg caggtgtaac caagggtata ataataatta ccctaaa 2337
<210> 4
<211> 966
<212> DNA
<213> 苏云金芽孢杆菌
<400> 4
atgaatttct tgtacaattt tgtgacatta gatatgctaa tattaaatag attagaagga 60
agtgatttta aaatgaagaa aaaagcaata gtgtgtggct tactagctag tactttatta 120
ggcggcggta cttttgtaga tgctgtgagt gcggctgaaa ttcaaaaaac taatcattta 180
aacaagtatg atagtgcaca agagaaagct ctacaagata ttaaccaaga agcgttacaa 240
gatattgatc aaaaagtcaa taagatgatt gattctatcc cacccatttt tggatcaaaa 300
tatacacgta cagatcgcta cggtgaaagt cttacttatt caggaataaa tctaaaagaa 360
aataatagta caaatgttga accgatgtac tttggttcaa atacatttta taacgataca 420
gagctagaac aatcctataa cactacttct tttagtgaag ctgttactaa atcaactact 480
actcaaacac aaaatggatt taaatcaggt gtaactacag gaggaaaggt tgggatacct 540
tttgtagctg aaggtgaagt gaaaatcaat cttgaatata attttacaca caccaattca 600
aatactacta gcaagactac aactttaaca gcccctccac aacctgttaa ggttcctgca 660
ggtaaagttt ataaagcaga cgtttatttt gaaaagaaat ctacttcggg tacggttgaa 720
ctttatggag atctccttac aggtgtagta gccgagggaa ggacatcatt tgtaggtaac 780
gtattacata aggcaactga tacacaaggg ctaattcaat ctcctgagga ttcaaataaa 840
gttcgtgcgg ttggaaaagg aacgttcacc actgaacatg gctcaaactt tatcgtcaaa 900
acatatgatg taacatcagg gcaaaaatcc gcaaaattgg tagatactag agtaatacct 960
ataaaa 966
<210> 5
<211> 969
<212> DNA
<213> 苏云金芽孢杆菌
<400> 5
atgaatttaa ggaggatttc tatgagagtt tataaaaaat tagcaacgtt ggcacctatt 60
gctgcattaa gtacatccat tttatgttct cctgcaatga catttgcagc agaaaaagaa 120
tcaacagtga aacaaaccac acaacaaagt gcggttcaac aaggtcgtat cattcaaggg 180
tatcttatta aaaatggtgt gaaaatacca gtttatacag gtggattagt aacaaataag 240
gctgaacaag gtgcagcagc atttccacaa ttgtcttcca atcctaatga tcctatccct 300
caaaaaggtt ctatttcatc tgaagatgga aatattggag atattttata tttttctaaa 360
actccaatgg gagataatgt ttatataaaa aaacttgaga ataacaacat tgaaattgga 420
aaatataatc gaggcacttt agaattatcg aaatttgtga cagttaatgg agatccacag 480
ggacctataa tgttatttga tgctacggta aaacgtgaaa cggcatttga aaaaattggt 540
ggtgctgtac aaccaaaggc aacacaatat acttttagtc aagcggtaac atccggttta 600
tcaacatcag atgcgattgg cggttcatta acattaggat ataaaatatc gcttaaagaa 660
ggtggcggag tagtaccagc cgaagcaaca caggaattta gtacacaatt aagtgctaca 720
tataatcata caattacagt gacaaaccaa acaacgaata cacaaacaca aacctttaaa 780
cctatagaca gttatggaca atcaacctat gcagctgctg tgtatcaatt aaaatctcat 840
tatacggtga ttccaggagc aggattacag aagggattaa atagtggata tgtgttagat 900
caaacagcgt tttcatatag cgattctgat ttatatctag ctgtaacacc aggagcaggt 960
tcaaatgta 969
<210> 6
<211> 756
<212> DNA
<213> 苏云金芽孢杆菌
<400> 6
ttgatcacta atcaagcagc acaagcaagt gatgcaccct atccagaatt accatcgaat 60
ccgaatgatg cgattccaaa tgccggggcc actcatgctg aaaatggaag tgtaggttcg 120
gttttatatt ttaaacagat agatttaaat aatctgggag ctggcatagg aaatagtcaa 180
aaagattatg tttacgtaga aaaaaaaggt gattctggat atgaattagg aaattacaat 240
ccgttaactt tacaaagaac taaaattaaa gattatgata aatccagtga acttgcagaa 300
aagatggatg gctattttaa aagtacaatt acacgagata ctttttttag taaaatcgga 360
tctggtgtcg taccaaaaaa tgcagcgtat acctttagtc aagcagttac atccggttta 420
actacatcag atgcgattgg gggtgcgcta acactcggat ataaagtaag tgttacagaa 480
ggcggaggaa tattcccagc tgcagcgtca gaagaattta gtgcacaatt aacagcaact 540
tataatcata cgattactgt ttccagccaa gtaacaaata ctcaaacatt gggcattaca 600
aaagctgcag atggctatca atatgataaa tatgtaggcg ctgtatatca attgcattcc 660
aagtatacat ttaaacctag tgatgaatta caatttgcaa tgaattcacc ttttggatat 720
aaggtaattc ttaatcaacg agcacaatca ttccaa 756
<210> 7
<211> 4323
<212> DNA
<213> 苏云金芽孢杆菌
<400> 7
atgacaacaa taaatgaatt atatccggct gtaccttata atgtactggc atatgctcca 60
ccacttaatt tagctgattc gacaccatgg ggtcaaatag ttgttgctga tgcaattaaa 120
gaagcttggg ataattttca aaaatatggt gtattagatt taacagctat aaatcaaggg 180
tttgatgatg caaatacagg ttcttttagt tatcaagctt taatacaaac tgttttgggt 240
attataggta caattggtat gacagttcct gtggctgctc catttgcagc tacagcgcct 300
attattagtt tatttgtagg atttttttgg cctaaaaaag ataagggacc acaattaatc 360
gatataattg ataaagaaat taaaaaatta ttagataagg aattaggaga gcaaaaacgt 420
aatgatttag ttagtgcttt aaatgagatg caagagggag caaatgagtt aagtgatatt 480
atgactaatg cactttttga aggtactata cagggaaatg ttgttactaa tgataaccct 540
caaggtaaaa ggcgaactcc taaagctcca acagttagtg attatgagaa tgtttattcg 600
gcatattttg tggaacatgt ggattttaga aacaaaatat ctacgtttct tactggttct 660
tatgatctta tagcactccc attatatgca ttagcaaaaa caatggagct ttcattgtat 720
caatcattta ttaattttgc taataaatgg atggattttg tatatacaaa agcaattaat 780
gaatcagcaa ctgatgatat gaaaagagat tatcaagcga gatacaatac tcaaaaaagt 840
aatttagctg tacaaaaaac acaattgatt aacaaaatta aagatggtac agatgctgtt 900
atgaaagttt ttaaagatac caataattta ccttcaatag gtactaataa attagcagta 960
aatgctcgta ataagtatat tagggcctta caaataaatt gtttagattt agttgctttg 1020
tggcctggct tatatccaga tgaatatctt ttaccattac aattagataa aacacgtgtt 1080
gtattttctg atacaatggg acctgatgaa acacatgatg gtcaaatgaa agttttaaat 1140
atattagact caactacaag ttataaccat caagatatag gaataagtac aactcaagat 1200
gtaaattctt tattatttta tccaagaaaa gaactgttag aattagattt tgctaaatat 1260
atttcatcta gtagtcgttt ttgggtttat ggatttggct taaaatattc agatgataac 1320
ttttatagat atggtgataa cgatccaagc agtgatttta aacctgcata taagtggttt 1380
acgaaaaatt cccagttcga aaaccttcct acttatggaa atcctactcc tattactaat 1440
ttaaatgcta aaactcaagt aacttcttat cttgatgcat taatatatta tatagacgga 1500
ggaactaatc tatataataa tgcgattctt catgatacag ggggttatat tccgggatat 1560
ccaggtgtag aaggatatgg tatgagtaat aatgaacctt tagcaggaca aaaattaaat 1620
gctttatatc ctataaaagt ggaaaatgta agtggttcac aaggaaaatt aggaacaata 1680
gcagcttatg ttcctttaaa tttacaacca gaaaatatta ttggtgatgc tgatccgaat 1740
acaggttttc cccttaatgt aattaaagga tttccatttg aaaaatatgg acctgattat 1800
gagggacgag gaatttcggt tgtaaaagaa tggataaatg gtgcaaatgc tgtaaaattg 1860
tctccaggtc aatcagttgg ggtacaaatt aaaaatataa caaaacaaaa ttatcaaatt 1920
cgtactcgtt atgcaagtaa taacagtaat caagtatatt ttaatgtaga tccaggtgga 1980
tcaccattat ttgcacaatc agtaacattt gaatctacaa caaatgttac aagtggccaa 2040
caaggcgaaa atggtagata tacattaaaa actatttttt ctggtaatga tctacttaca 2100
gtagaaatcc ctgttggaaa tttttatgtg catgttacga ataaaggatc ttctgatatc 2160
tttttagatc gtcttgagtt ttctacagtt ccttcatatg ttatatattc aggtgattat 2220
gatgctacag gtacagatga tgtcttattg tcagatccac atgagtattt ttatgatgtc 2280
atagtgaatg gtactgctag tcattctagt gcagctactt ctatgaattt gctcaataaa 2340
ggaaccgtag taagaagcat tgatattcca ggtcactcaa cgtcttattc tgtacagtat 2400
tcagttccag aaggatttga tgaagttaga attctcagtt ctcttccgga tattagtgga 2460
actataagag tagaatctag taaaccacct gtatttaaga atgatggtaa tagtggtgat 2520
ggtggtaata ctgaatataa ttttaatttt gatttatcag gattgcaaga tactgggctt 2580
tattctggta aacttaaatc tggtattcgt gtgcaaggta attacactta cacaggtgct 2640
ccatctttaa atctggttgt ttacagaaat aatagtgttg tatccacttt tccagtaggt 2700
tctccttttg atatcactat aacaacagaa actgataagg ttatcctttc attacaacct 2760
caacatgggt tggcaacagt tactggtact ggcacaataa caattcctaa tgataaatta 2820
gcaattgttt atgataagtt atttaaatta ccacatgatt tagaaaatat aagaatacaa 2880
gtaaatgcat tattcatatc gagtacacaa aatgaattag ctaaagaagt aaatgaccat 2940
gatattgaag aagttgcatt gaaagtagat gcattatcgg atgaagtatt tggaaaagag 3000
aaaaaagaat tacgtaaact ggtcaatcaa gcgaaacgtt taagtaaagc acgaaacctt 3060
ctggtaggag gcaattttga taattgggaa gcttggtata aaggaaaaga agttgcaaga 3120
gtatctgatc atgaattatt gaagagtgat catgtattat taccgcctcc aactatgtat 3180
ccatcctata tatatcaaaa agtagaagaa acaaaattaa agccaaatac tcgttatatg 3240
atttctggtt tcatcgcaca tgcggaagat ttagaaattg tggtttctcg ttatgggcaa 3300
gaagtaagga aaatagtgca agttccatat ggagaagctt tcccattaac atccaatgga 3360
tcaatttgtt gtacaccaag ttttagacgt gatggaaaac tatcagatcc acatttcttt 3420
agttatagta ttgatgtagg tgaactggat atgacggcag gtccaggtat tgaattggga 3480
cttcgtattg tagatcgatt aggaatggcc cgtgtaagta atttagaaat tcgtgaagat 3540
cgttctttaa cagcaaatga aatacgaaaa gtgcaacgta tggcaagaaa ttggagaacc 3600
gaatatgaga aagaacgtgc agaagtaaca gcattaattg aacctgtatt aaaccaaatc 3660
aatgcgttat atgaaaatgg agattggaat ggttctattc gttcagatat ttcgtactac 3720
gatatagaat ctattgtatt accaacatta ccaagattac gtcattggtt tgttcctgat 3780
atgttaactg aacatggaaa tatcatgaat cgattcgaag aagcattaaa tcgtgcttat 3840
acacagctgg aaggaaatac actattgcat aacggtcatt ttacaacaga tgcggtaaat 3900
tggatgatac aaggagatgc acatcaggta atattagaag atggtagacg tgtattacga 3960
ttaccagact ggtcttcgag tgtatcccaa acaattgaaa tcgagaaatt tgatccagat 4020
aaagaataca acttagtatt tcatgcgcaa ggagaaggaa cggttacgtt ggagcatgga 4080
gaaaaaacaa aatatataga aacgcataca catcattttg cgaattttac aacatcacaa 4140
agtcaaggaa ttacgtttga atcgaataag gtgaccgtgg aaatttcttc agaagatggg 4200
gaattattgg tagatcatat cgcacttgtg gaagttccta tgtttaacaa gaatcaaatg 4260
gtcaatgaaa atagagatgt aaatataaat agcaatacaa atatgaataa tagcaataat 4320
caa 4323
<210> 8
<211> 1092
<212> DNA
<213> 苏云金芽孢杆菌
<400> 8
atgttaataa aggagatgca atatatgcat tctattaaaa aatataaaaa ggttctatta 60
attgcaccac ttgcttgtat gttaacaggt gctattttac ctacagctac tacagttcat 120
gcacaagagg tagaaaataa aaaagctgta tcaatgatga agccgggagg agagtttgga 180
gcaactaaat attcaaaaga aaatttagta aaggaaatca atcttagatt attaacagcg 240
cttgatcgtt caacaagttt gcgtgaaaaa tttcatataa agggcaacga agttttagat 300
gttagtcagc ttgatgacac atctaaacaa ttaatggaga aattacaatt aacagctgaa 360
ggatcaattg atgtgaaacc acatgtcgat agctataaag atcttggtca aaccaatatt 420
gttacatata acaatgataa cggagtggtt ggacagacat ataacacacc agaaacaaca 480
gtaaaagaat ctgaaactca tacctactcg aatacagaag gggtgaaatt aggactcgag 540
gtaggaacaa aaattacagt tggaattcca tttatcggaa aagatgaaac agaaataaaa 600
gcaacatccg aattttctta tgaacataat gattcacaaa caaaaacgaa agaaactgat 660
gttacgttta aatcccaacc agtagttgcc gctccaggtg gaacaaccac ttattatggt 720
gatattaaaa cagcaacatt ttctggatca tttcaaagtg atgcttatgt agcaggcggt 780
ttcgaattga aagttcctat cgcacatgat atggcttcgc caaaaataga tcgttatgaa 840
acggctacgc tgacagctgc agatatatat gaaattttta atgcttctaa tgcgatagca 900
gcaccaaatt acttaaaact tgataacgca ggtaaaaaag ttcttcttac agacaaagca 960
acttttgata taaatggaca aggtggtttt tatacaacat tacaggttaa atttgttcct 1020
aaagattcta ataaaaagcc tcaaatgatg tcttataaag aatatgtaca aaaaatgaac 1080
aataatgaat ta 1092
<210> 9
<211> 1182
<212> DNA
<213> 苏云金芽孢杆菌
<400> 9
atgtattcta ttaaaagata taaaaaggta gcaatagtag ccccacttgt ttgtttattg 60
ggaacgggac taacatttgt taataaacca ataccagctg ctgcggcagt aactacaaat 120
tattctacag cagattctgc atcaaatttc caacccatta gtaaatatac tttagccgga 180
gatctatatg aacgatatat gagagctcta gtaagacatc ctgaattact ttcttcaggt 240
ggtttaaaac cagtaactaa tcaaacggat ctagaacaaa tcgacggata ctacaaggta 300
atggctcaat tcataagaga caataatcag aattttccat ctccttttaa tagaccaagt 360
atgaaattga tgactggagt taatccgttt tttaattggg ctcctcaata tactaatctt 420
tctactcaaa atgtaattaa cttagataat ccaaaagtag atgattataa agaagataat 480
attgaactag ctacttatac taataacaca acatcagaac aaactttttc gacgccttca 540
aaatcagaaa aagtaacaga ttctttcaca tattctaatt cagaaggtgg aaaattagga 600
gtttcttcca cgactacaat tagagcggga attccaatag cgcaagctca agaaactctt 660
acaatgtcat ttgaagcaac ttataatcat acaagctcga atacatcttc tactgaaaag 720
acagttacat atccatctca agtactaaag tgcctaccag gatatagaac ttctttaatt 780
gtaaaagtat ctcaagcgaa tttttctggt acaatggatt ttgacgttga accaactgtg 840
agttcattaa tagatggtat agaaaaaaat tggaaagaca taaaagacga taagacaata 900
aaaggagata aaagtggaga ttacacagtc ccaaatcgac aagaattttt atataatgtg 960
tataaatatt cagatttacc aattccatct tatgttaaat tagatgataa aaagaaaact 1020
gtatcatttg gaaaagttac aactccatat acaggtgtag caggtcattt atcagaagca 1080
aatgcaacac aagtaaaact ggaatcactt gataaagcac agaaaccaat tattatgcct 1140
ttaaaacaat atcaacaaaa aattcaaaat catgaatctt tt 1182
<210> 10
<211> 1080
<212> DNA
<213> 苏云金芽孢杆菌
<400> 10
atgcattcta ttaaaaaata taaaaaaatt ctattagttg caccacttgc ttgtatgtta 60
acaggtgcta ttttacctac agctactaca gttcatgcac aagaaatcaa gggaccgggg 120
gtaatgaaac ctgacgttcc gtggaatcaa gaacattata cgaaagaaaa tttagcatgg 180
cgtgcagcag atagactttc ctatgctgcg gatagaattc ctagtttacg tgagaaattt 240
aaattaaaac caaacgaaca tttttattgt agcaatgaca cgaggtacta tatggaagaa 300
accttattaa aaaacttgca attatcagct gaaggtccaa taaatgttac accacatgta 360
gatagttata ctgatttagg acaaacaaat ttattaacat ataacaatga tgatggaatt 420
gtagagcaaa aggcttctac accagaaact accattaaag aatcagaaac atcttcttat 480
tctaataaag aaggagttac attgggagca gaggtggaat ctaaagtaac attcaatata 540
ccatttatag tagctggaga aacaaaggta atagcgaaat ccgaattttc ttatgaacat 600
gatgatactc aaactaagac ccatgaaaaa gaggtaacat ttaaatcaca agagatcgtt 660
gctgctccag agggaacaac tacttattat ggttcaatca aaactgccaa tttttctgga 720
tcgttccaaa gtgatgctgt agtaggtggt ggtgtaacgt taacccttcc tataggagta 780
atggataagg atggtgggca gaaaaaaact catacggaaa cagctacttt aactgcagaa 840
gatatgtatg agattttcaa agcaccaatg ccttgggaca tgaataaatt accaccatat 900
ctaaaattag atgattctgg caaaagggtg ctactggcag aaaaagcaac ctttgatata 960
aagggacaag gtggttttta tacagaaata caggcaaaat ttgttccaaa agacaaaaat 1020
aagaaaacac aaattatgcc atacgcagag tatgtacaga aagtaaaaca gaatgctctt 1080
<210> 11
<211> 2028
<212> DNA
<213> 苏云金芽孢杆菌
<400> 11
gtgaaaagta tgaattcata tcaaaataaa aatgaatatg aaatattgga tgcttcacaa 60
aataactcta ctatgtctac tcgttatcca aggtatccac tagcaaagga tccacaagct 120
tctatgcaga ctacgaatta taaagattgg ctaaatctat gcgatactcc aaatatggaa 180
aatccagagt ttcaatcagt aggaagaagc gcactttcta ttctcattaa ccttagctct 240
agaatattat ccttattagg tattcctttc gcagcacaaa tcgggcaact ctggagctat 300
acactcaacc tactatggcc tgtggcaaat aatgctactc aatgggaaat ttttatgcgc 360
accatagaag aattaattaa tgcacgcata gagacttcgg taagaaatag agcccttgca 420
gagctggcag gcttaggaaa catattagag gactataagg tggttttaca acgatggaat 480
ctaaatccta ctaatccaac attgcaacgc gatgtggtac gccaatttga aatcgttcat 540
gccttttttc gctttcaaat gccggtcttt gctgtagatg gttttgaagt accattattg 600
ccagtatatg cttcggcagc taatcttcat ttgcttttac taagggatgt tgtaattaac 660
ggagctcgtt ggggcttgga atctgatgta attaacgatt atcatgacct tcaattacgt 720
cttacatcaa catatgtaga ccattgcgta acttggtaca acactggatt aaacaggtta 780
attggcacaa atgctagaca atgggtaact tacaatcagt tccgtaggga gatgactata 840
tccgtactag atattatttc attattttct aactatgatg ttcgtagata cccaacaaaa 900
acacagagcg agctaacaag gatgatttat acagatccaa taggtaccga agggaatcaa 960
tttattcctg ggtgggtaga taatgcacct tctttctcgg ttatagagaa tagtgtagtt 1020
cgaagcccag gagctttcac ttttctggaa agggttggta ttttcacagg gttcttacat 1080
ggatggagta gccggtctga gttttggtca gcccatagat tattttctag accggttttg 1140
ggttggatat gggagagtgt tatttttggc aatccccaaa ataatattgg gtatcaagaa 1200
gtggatttta cgaattttga tgtatttagc attaattcta gggccacttc tcatatgttc 1260
ccaaatggca gtgctagatt atttggagtt ccacgagtta catttgattt atcgaatgta 1320
actaataata atctagcaca aagaacttat aacagaccat ttacttttgg cggccaggat 1380
atagtgtcga gattacctgg cgaaacaaca gagataccga atagtagtaa ctttagtcac 1440
agactagccc atatttcatc ttttccagta ggtaacaatg gatcagtcct ctcatatggg 1500
tggacacacc gtaatgtgaa tcgtcataat agactgaatc cgaacagtat tacacagatt 1560
ccagctataa agtttgctag tggttctgca cggagaggtc ctgggcatac aggtggagat 1620
cttgcaattg ctcaacaaca cagtggttat cagctgttta tgcaatcgcc ttcagcacaa 1680
aggtaccgtc tccgtttgcg ttatgccggt atttcgggag gtagtatttc tgtttcgcat 1740
cgggacgaaa ataatcaaaa catccttcat agtgctacat tcaatgttag ggctacatca 1800
ggtcagctaa gatacgccga tttcatttat acagacctag aggagaacac aacgttgttt 1860
gaaactcgaa atggagtgaa tctatataga ctaatgattt ttgtttcaag tggctctata 1920
ttaattgacc gaattgagta tatccctgaa aatacaacaa ctatagaata tgaggaagaa 1980
cgaaatctag aaaaagaaaa gaaagcggtg gacgatttgt ttaccaat 2028
<210> 12
<211> 1701
<212> DNA
<213> 苏云金芽孢杆菌
<400> 12
gtgaataata tgtataccaa taatatgaaa actacattaa aacttgagac gacagattat 60
gaaatagatc aagcggcaat ttcaatagaa tgtatgtcag atgaacaaga tctacaggaa 120
aaaatgatgt tatgggatga agtaaaactt gcaaaacaac tcagtcaatc tcgtaattta 180
ctctacaatg gtgattttga agattcatcc aacggctgga aaacaagtaa taatattacg 240
attcaattgg agaatcctat tttaaaaggg aaatacctca atatgcctgg agcacgagac 300
atatatggaa ccatatttcc aacatatgtt tatcaaaaaa tagatgaatc taaattaaaa 360
cccaatacac gttatcgagt aagaggtttt gtgggaagta gtaaagatct aaaattagtg 420
gtaacacgtt atgagaaaga aattgatgct agtatggatg ttccaaatga tttgtcctat 480
atgcagccta gcccttcatg tggggattat ggctgtgact catcatccca gccaatgatg 540
aatcaaggat atcctacacc atatacagac gactatgctt ccgatatgta tgcatgctcg 600
tcaaacctag gtaaaaaaca tgtgaagtgt cacgatcgtc atccatttga ttttcatatt 660
gacaccggag aattagatac aaatacaaac ttaggtattt gtatcttatt taaaatttcc 720
aatccagatg gatatgctac attaggaaat ctagaagtaa ttgaagaagg accactaaca 780
agcgaagcgt tagcacatgt gaatcaaaag gaaaagaaat ggaatcaaca aatggagaaa 840
aagcgatcgg aaacacaaca agcctatgat ccggcaaaac aagcagtaga tgcattattc 900
acaaattcac aaggagaaga gttacactat catattactt tagatcatat tcagaacgcc 960
aatcagttgg tacagtcgat tccttatgta caccatgctt ggttaccgga tgccccagga 1020
atgaactatg atttatataa caatttaaag gtacgtatag aacaagcacg ttatttatac 1080
gatgcacgaa atgtcataac aaatggcgac tttgcacagg ggctaacggg gtggcacgca 1140
acaggtaaag tagacgtaca acaaatggat ggagcttctg tattagttct atcaaactgg 1200
agtgcggggg tatctcagaa tctgcatgcc caagatcatc atggatatat gttacgtgtg 1260
attgccaaaa aagaaggtcc tggaaaaggc tatgtaacga tgatggattg taatggacat 1320
caggaaacac tgaagttcac ttcttgtgaa gaggggtata tgacaaaaac agtagaggta 1380
ttcccagaaa gtgatcgtgt acggattgaa ataggagaaa ccgaaggtac attttatata 1440
gatagcatcg agttgctttg tatgcaagga tatgctagca ataatacccc acacacaggt 1500
aatatgtatg agcaaagtta taatggaatt tataatcaga atacgagcga tctgtatcac 1560
caagggtata caaacaacta taaccaagaa tctagtagta tgtataatca aaattatact 1620
aacaatgatg accagcattc cggttgcaca tgtaaccaag ggcataattc tggctgtaca 1680
tgtaatcaag gatataaccg t 1701
<210> 13
<211> 1920
<212> DNA
<213> 苏云金芽孢杆菌
<400> 13
atggaagaat tagagttaaa aagaacaaac acactatctt ctgaggatgt gaatatttta 60
caaattgaaa atttagtaaa agaatatgtt aagcaaacat atggtaattc agctgaaatc 120
aaaaaacttt cattagatgg gttagatgtt ttgtataatt tagatattcc ttctatttta 180
aagggtactt cttcttcttc tgctattaaa gttggtactg acaatttgaa taacccaaca 240
gatacagcga aaaccattaa acttccagtt aaaaatgtac gaaaaaaaga atttaaggtt 300
aaacctattc aagctttaaa ttttgaaaat ggtgcaacaa tcactaaaaa aagtataact 360
tcaattccta gtattaatgc tacttttata gctttggctg aacagaattt tcaaaatgca 420
cattttcata tagtaaacga tagtcaatct tatgaaaatg aaatacccat ctatgtacct 480
ccacattcaa aagttgaaat aacatactac gtgaaagaaa tccaatttga tgcaattatt 540
cagtccactg ccacaatagg tggatccata agttttgaat atattgttca tgataatggc 600
catgaaggaa tagattttct tactattttc gaattagtaa atagccttaa tctaaatgac 660
tttgaaattc aggaggcatc tgatgtacat ggtaaggtag tttataaagg gaaatcccaa 720
tttcagggaa ctgtaggttt aaatttattc atgcaaatca aaggaacgcc attggatgaa 780
agtaaaaata actatgaatt taccaaagta ctatcagagg acgttgaaat gagtctatct 840
ccgtcggagg gagaatatta tattgatttc gggtcatctc ctaaattaac aaataaagaa 900
gaagtaatag ttaaatttac aagagattac cttttgtcaa atgaccgcaa aaacgcatac 960
gtacaacaac taccacgtct tgaatatgga gaagaagtta ctacattaaa atctattgat 1020
actgctcatg aaaggaaaga aatcatagct tctacaatta atacttttca aaatccttct 1080
gatacagaga ttaccagaaa tacaataaaa gagacattta gtacaacgga tacaattact 1140
accactgcta caactgataa gtttcttgaa ctgggaggaa gtatagaaac ctctgcaaaa 1200
ggaaaagttc cactagtcgc agaagcatct ataaaagtta cacagagtat aaaaggtggt 1260
tggaaatggg taagtacaaa aactaataca agaacaaacg tccatacaat tgaaatacca 1320
tcgcaatcta ttaaaatacc tccacacaaa atgtggaaat accagtatat tctaacaaaa 1380
ttcgaatcaa gtggttattt aagctcagct tgggaaataa atactaaaga atctatgtca 1440
gctccggagg ttcacatagg ttactataat aaagatcttc agaatccaag aaatataacg 1500
gggctatcgg caaatgttga atctggaaat gtagttggac gtgtatttga attcaataaa 1560
ttccagccag gaggacttca ttacaaaata ctgaatagtg aaaatatatt aaatgcaact 1620
ccttatcaat tttttaaaga attagccaaa cgtgttaatc aatacccatt aatacaaaat 1680
aatcctcgct atagacgatt aggaattctt ttgggatttg gaaaggacat atctcaaatc 1740
acttgggaac ctcagataca ctataatgaa catgtgttat ttgatgctga agaactatta 1800
aatgttttac gttttgatga tattgccaat aaagtatatg cacttgatgg aggcacacct 1860
tttacagttg ctgtaggtca tgagctacta ccaaaagagt caatagaacc attaaataat 1920
<210> 14
<211> 2898
<212> DNA
<213> 苏云金芽孢杆菌
<400> 14
gtgatgaata tgagtaatac cttagcacct tataatgttt tgagaagtat ggatatgccg 60
aacatatcag gaaccaagtg ggataaagga atgtttatca atgcacttga taatacttct 120
tttcttttag agcttataga aaaaggaatt aatgatgatg atgatgtgtt aggtctgtta 180
agttttattg gattaacagc cttagaggca attccaattg tgggtggagt tatgtccaaa 240
cttgtttcta tgatattttt ccctacaaaa tcaagcatta atttccagaa gatatgggag 300
caattagaaa aagctattga acaaatagtt gacaaaaaaa taactgaagc tatgatgtct 360
cagctaatgc aagaaatagc cggtttagcc gatgtattag aagaatatag gaatgcttat 420
gatttatata atggtaaaaa attatttaat ataccagata agatgacacc tggggattat 480
ctgatcaatg tatttactac tgcaaatttg caattcattc agagaatacc gacatttcag 540
aactctatat atgatgtagt gtttcttcca ttctttgttc acgctgctga aatgcatatt 600
cttctgatta gggatgcagc aatacatggt caagaatggg ggatggatga aactgtacac 660
caaaaattta aaagggattt aaaaacttta attaataaat attctagtta tttattagct 720
acatataaaa aaggattaaa agaagcatcc gaaaaaaaac ttgaaaataa tgattttcca 780
acatctaaca accaacatca ttatattaat acagttagat ggaatgtaat caatcaatat 840
aaaagaggga tggctttaac tgtttttgat tttgcttaca aatggaagta ttaccaagaa 900
gtttatcaaa ataatataac gttaaatcct gctagaacaa tttattcaga tattgcaggt 960
tcggtatatc cttatgaaaa aactacaaat gaaattgata atattatcaa agagcagaat 1020
cttaaatatc gcggactctt aaaagaactg ctaattaatc atggggatag aatcgatagt 1080
attcaaagta agtatataag gaacaatgaa ataattgata gtaacagaac tgggggggct 1140
ggtggaaggg caaccttttt cgatttaaaa tctccaataa ataacccttt catacaagta 1200
aatatgtggt ctgaattagt accattctct ttaggattca aatattataa tggggaggag 1260
tcaaaactta tatggggagg agggacccct gggaaacata agtttggttc ttatcattat 1320
gtagggaata aagtgtcttc tattatagga tttggtaaaa atggaaccgg tggattcaac 1380
tctttagatg caatggtagt tggttttaaa cgagatgatt atatacctga aaatagattt 1440
gttggtgtaa acaaaaatgg tgaacctgta actaaagtaa tagatgcaga gaatttctac 1500
caagagaagt ttcaatcaaa tataaaaatg atagatgagc ctatgtttgg agaggcggtt 1560
ttacaattcg aaaattattc taataatctt aataaggata gttatgtgac atatcaaatt 1620
gatgcaaaga tagagggtac ttacgaatta catgtaatta taggtgcaaa aaaacaaaaa 1680
gataaaatag cttttaaaat ggctcttaat gaaaaacagc cagaaaagtt tataactgaa 1740
ccctttaatg ccggtgatat ttgggaagga atatcattga gcgaaggctt agtttataag 1800
agaatattat taggaaattt ccaacttaag aaaggtatga atcgtattac tattcacaat 1860
ggggtcctcc aaacatcagc aaatataaaa acatggaatt tagctaaact agagttaaca 1920
ctcacttctg atagcttaaa agaccctgat attacaactt tatatgataa agataattat 1980
tcaggaacaa aaaagtttat tttcgagaat acgagtcgtt taaaagactt taatgataaa 2040
acatcatcaa taaaagtcga gtctcattta gctggcatta ggatttatca agattacaat 2100
tataaaggta agtctatgga ccttgtaggt ggagaaaaaa taagtttaaa aaatcattca 2160
tttaataaca gagcttcttc agttaaattt gctaatatcg ttttatataa ccaagataac 2220
tatcagggtt caagaaaact agtttttgaa gatattcctg atttaggaaa acaaagcttt 2280
aacgataaaa cctcttcaat tgttgttagt tctaatgtat ctggtgctag actatatgaa 2340
catgcttatt ataaaggtaa gtatgtggat gttgttggtg gacagaaact taatttaaaa 2400
aatcatgtat taaataaaaa gatttcatcc attaaatttt ttaaagaggg tgaagtactt 2460
aatggtgtat atcaaattat tactgcaata aataacacga gtgtaataga taaacatcta 2520
gaaaattcta acgttcattt atgggagaat gcagaaaaca aaaatcaaaa atggcgaatt 2580
gagtatgatg tggctaaaaa agcttatcaa attaagaata tgttggatga gaagttagta 2640
ttatcaacgc atgagttatt tccaattttc tcagcactat attgtttacc taacaaaggt 2700
tatgtttcgc aatactggat ttttgagtat gtaggaaatg gttattatat tattaaaaat 2760
aaagcgtatc ctgattgggt attagatgta gatggtttga attctgataa tggtacttta 2820
attaaattac attcacagca tgatttaact gatccactta ttaatgcgca aaaatttaaa 2880
cttaaggata taaataat 2898
<210> 15
<211> 1725
<212> DNA
<213> 苏云金芽孢杆菌
<400> 15
atgaatggaa atggaagaca tgatggatgg aatcagaatc aacacataga aaatggacag 60
atgaacccaa atcatagtgg atcttgtaag tgcgggtgtc aacaaaataa taatggatcg 120
tatccttcga atgagtataa ttcaaataat aatggatcgt atccttcgaa tgagtataat 180
tcaaataata atggatcgta tccttcgaat gagtataatt caaataataa tggatcgtat 240
ccttcgaatg agtataattc aaataataat ggatcgtatc cttcgaatga gtataattca 300
aataataatg gatcgtatcc ttcgaatgag tataattcaa ataataatgg atcgtatcct 360
tcgaatgagt ataattcaaa taataatgga tcgtatcctt cgaatgagta taattcaaat 420
aataatggat cgtattcttc gaatgagtat aattcaaata ataatggatc gtatccttcg 480
aatgagtata attcaaataa taatggatcg tatccttcga atgagtataa ttcaaataat 540
aatggatcgt atccttcgaa tgagtatgta ggaggatata gtatacaaga tggtttacct 600
caagaaagta aacagtttca aaaaatttca aatatgaata ctagagataa tcatcgtgtt 660
ttagacgcac aagatactta ttttggtcaa ttgattgata atcgtgtagg tgacacatgt 720
aaatatgtag agcataaaaa ttcagtaatt tatgaactta gtaggcaacc tgtatatact 780
cctgattccc aatatttcat tttttatcaa atggataatg ggaattttat aattgcgaat 840
aaagaaaata gtcgagtttt agaagttata tttagttcag taaatggatt tgtaacaata 900
tcaaatgagt ttaatgcaac ttcagatcaa cgttttaagg ttgttagatc aaagaatgat 960
acattccgct tagtaacaga aggaaataaa acattaaata tatgtggtca ttcatttcaa 1020
tataatacta aaattacagc tgtaaatgcg gatattgatg gtgataatta tttatttcaa 1080
aaatctatgg ataaggatac aagggattta tatttcggaa caatatctaa taaaaatcca 1140
gaaatattaa atgacccaag aaatttaaaa agtttagatg atcttggtga tgagccgaga 1200
gcatttaaag gagccgcatt acttcctgct ctatttgtaa atgaccctag atattcagtg 1260
catcgaagag tatcaaatag tccatattat tacttagagt atacacagta ttggcataga 1320
atatggactg atgttttgcc tattgatggt tatggcgcat ggatagaaat gataggggta 1380
acaaatgata cacaagtaaa tatgaaaaac ataatgaata ttacaataac tggaaaagat 1440
ttaggtgtag atttgggtat agatttggga ttaagatttg gtgataagtc atttcttttt 1500
gaacaaaaaa tcctttcagg attatctata cggaaaactg attatccaaa tctcggaata 1560
gatgaaagag caatgtatca aagaaacaat agtaatttaa aaaccagatt tgtaagatac 1620
gtgaaaaaac atgaatttgt attaagagat ttgaatggga gtaaggtagc tgaaccatgg 1680
attatcacgg aagatagatc gattacgaaa gaatactctt caaat 1725
<210> 16
<211> 1677
<212> DNA
<213> 苏云金芽孢杆菌
<400> 16
atgaagtaca aaaatcgaac acgtgcaaaa tgcaaataca aacaagcgct tcttgtaaca 60
gtagcaacaa tgacactagg agtaagtaca ttaggaagca atgcctcagc attcgctgat 120
gaaaaagaaa agaatgttat tcagcaaaaa agtcctggca cttattatga agatgcgcaa 180
aaaaatctgg gctccctagc acgatttgat acatgggcac aagatcttgg aaaaacaaca 240
ggtgcaggga attataaaac tacacttggt atggcagaaa agctactacc aacaatttat 300
aatgatttaa atagtggaaa tttcaacaat actgcaaggt ccattacgat gttatctaca 360
gcgttgattc catatggagg agcattcatt tctccgataa ttgggatact ttggccagaa 420
aacgggccaa atataaaaga aatgctgcag gaaatggaaa acaaacttgt tggtataatg 480
gatgaaaaaa ttgaagccaa agatttagat gatcttgagg ctgcagtaaa aggattgatg 540
gtaagtctaa aagaatttga aaactcattg aatggtaata taggtggtga atattattct 600
gccctagctg atgtagactc acttaaccga ggtcgtataa cagccattca aaagggtttt 660
aacgacctta ttagtgctac tagtaaaccg aagttcaaaa taacagaact tcctctatat 720
acaattattg caactgctca cttgaatttc ttgaatactg tggaaaaaca gggaacttca 780
cctaaaataa actacacaga agcagcctta aaagatcttc tacaaaatat gaagaagaat 840
cacaaggatt atgcagatta tatagaaaaa acgtatacag aaggagaagc tagaatcaat 900
agcaaactag aagataaaca aaaaatagaa caagatttag ctgccgtaaa ccaaaagcta 960
tcggaaatgc ctcgtaaacc taagaatcac acccacgagg aagagaataa atttataatt 1020
caaaaagaga agctttacgc gcaacaagac tctcttgaga aaaagttgtc tgaatacaat 1080
gacttgatgt atcaaaagag tgatttttat agcaagacaa agggtagcga agcattccaa 1140
atagcatcaa caggaaaaac gataccaact ccaagttggg ttaaaacaga aggaacgtgg 1200
gtttgcgagg ctggtttttg gttttatatt gacgcaaaag ggcaaaagaa aagtgattgg 1260
ttcaatgata agacacctga tggtaaggat agatggtatt accttagcac tgaaacacca 1320
cgtctcgaca atgtaagggg gaatgcttat gttggaaaag gaacaatgct gactggttgg 1380
ttccacgata cgcgtaaaga taagcagatc atcggtgtga atacgaagac tacttatgaa 1440
tactggtatt acctcagccc agaaaaaaat cttaaaaact ctgccggaga actatttaag 1500
cagggacaga tgatgacgaa atgggttgaa attaaggata caaagactgg tgaaccacac 1560
tggtattatt tcaatcctga cgacggtagt atgacacatg ataaaaaagc ggtacaaatt 1620
ggtgacaaaa aatatgattt tggttccaat ggtgtatgta caacgcctaa cggttac 1677
<210> 17
<211> 3879
<212> DNA
<213> 侧孢短芽孢杆菌（Brevibacillus laterosporus）
<400> 17
atgaatcaga atcaaaataa aaatgaaatg caaattatag aaccttcaag tgattctttt 60
ctttatagtc acaacaatta tccgtatgcc actgatccaa atacagtatt agaaggtagg 120
aattataaag agtggctaaa taagtgtaca gataattata cagacgcttt acagagtccc 180
gaagctactg ctatttcaaa aggagctgtt tctgctgcga tttctataag caccaaagtt 240
cttggtttat taggtgttcc atttgcagct caaatcgggc aactttggac cttcatatta 300
aatgcgttat ggccttcaga caatactcaa tgggaagagt tcatgagaca tgtagaagaa 360
ctcataaacc aacgaatagc cgattatgca agaaataagg cacttgcaga attaacgggt 420
ttaggtaata atctagattt atatatagag gctcttgatg attggaaacg aaatcctact 480
agtcaagaag ctaaaacccg tgtaatagat agattccgta tagtagatgg attatttgaa 540
gcatatatac cttcatttgc agtatcaggt tatcaagtac aattattaac ggtgtatgca 600
gccgctgcaa atctccactt acttttatta agagattcca ctatttacgg aattgattgg 660
ggattaagtc aaactaatgt taatgataat tacaatcgcc aaataagact caccgcaacg 720
tatgcaaatc attgtacaac ttggtatcaa actggtttag aaagattgag gggttccaat 780
gcttccagtt gggtcactta taataggttt cgaagggaaa tgacgttaac cgtattggat 840
atttgttcct tattttcaaa ttatgattat cgtagttacc cagcagaggt aaggggagag 900
attacgagag aaatttatac agacccagta ggtgtaggct gggtggatag tgcaccatca 960
ttcggagaaa tagaaaatct agcaattagg gcaccaagaa ccgttacttg gttaaattca 1020
acaagaattt ttacagggag attgcagggc tggagtggta ctaacaatta ttgggcagct 1080
cacatgcaaa acttctcaga aaccaattca ggaaatatac aatttgaagg tcctctctat 1140
gggtcgacgg taggtactat tcatcgtact gatgattacg atatggggaa tcgagatatt 1200
tacaccatta cttcacaagc tgttttaggc ctttgggcaa ctggtcaaag ggtgttgggg 1260
gtcgcttcgg ctagatttac tttaagaaat cttttcaata atcttacaca ggtgctggtg 1320
tatgagaacc caataagttc aacttttgga agttcaactt taactcatga attatctgga 1380
gaaaactcag ataggccaac ttctagcgac tatagtcata gactaacgag tatcacaggt 1440
tttcgagctg gagctaatgg aacggtccca gtgtttggtt ggacatctgc aactgttgat 1500
cgtaacaata taattgagcg aaacaaaata acacaattcc caggtgttaa gtcacacact 1560
ctcaacaatt gtcaagtagt taggggtact ggatttacag gaggagactg gttgagacca 1620
aataataatg gtacatttag actaactatt acttcattct ccagccaatc ttaccgaatt 1680
cgcttacgtt atgctacttc agtagggaat acttctttag ttatatcttc ttctgatgca 1740
ggtatttctt ccacaacaat tccgcttacc tcaacaataa catcactgcc acaaactgta 1800
ccataccaag cttttagggt tgtagattta cctattactt ttacaacacc tactacccaa 1860
agaaattata cgtttgattt ccgtctccaa aatccatcaa acgcaaatgt attcattgat 1920
agatttgaat ttgttccaat tgggggttct ttgtctgagt atgaaaccaa acatcagcta 1980
gaaaaagcaa ggaaagcggt gaacgatttg tttaccaatg aatcgaaaaa tgtgttaaaa 2040
aaagacacga ccgattatga tatagatcaa gctgcaaact tggtagaatg tgtatctgat 2100
gaatgtgcaa atgctaaaat gatcctatta gatgaagtaa aatatgcgaa acaacttagc 2160
gaagcccgca atctacttct aaatggtaat tttgaatacc aagatagaga tggggagaat 2220
ccatggaaaa caagtcccaa tgttaccatc caagagaata accccatttt taaaggccgc 2280
tatctcagta tgtcaggtgc gaacaatatc gaggcaacca atgagatatt tcccacttat 2340
gtataccaaa aaattgatga atccaaatta aaaccttata cccgttataa agttcgaggt 2400
tttgttggaa atagtaaaga tttagaatta ttggttacac ggtatgatga agaagtagat 2460
gcgattttaa atgtaccaaa tgatatacca catgctccgc cacctttctg cggtgaattt 2520
gatcgatgca agccgcattc ttatcctcct attaatccag aatgtcacca tgatgtaata 2580
aataacattg aaatatcctc tccttgccaa cacaataaga tggtagataa cgctgatata 2640
tcttatcgcc atagccgatt aagtaaaaaa catggcattt gtcatgaatc tcatcatttt 2700
gaattccata ttgatacagg gaaaatcgat ttggtcgaaa atttgggaat ttgggttgta 2760
tttaaaatat gttccacaga tggttacgca acattagata atttggaagt tattgaagag 2820
ggtcctttag gagccgaatc cttagaacgt gtgaaaagaa gagaaaagaa atggaaacat 2880
cacatggaac acaaatgttc agaaactaaa catgcatatc atgccgcaaa acaagcggtg 2940
gtggcgttat tcaccaactc taaatatgat agattaaagt tcgaaacaac catatccaat 3000
attctttttg ctgattatct cgtgcagtca attccgtatg tatataataa atggttacca 3060
ggtgttccag gtatgaatta cgatatctat acagaattaa aaaatctgtt tacgggagct 3120
ttcaatctat atgatcagcg aaatattata aaaaatggag actttaatcg tgggctcatg 3180
cattggcatg cgacacctca tgcaagagta gagcaaataa tagataatag gtctgtgcta 3240
gtgcttccaa attatgctgc caatgtttca caagaggttt gtttagaaca caatcgtggt 3300
tatgtattac gtgtaacggc gaaaaaagaa ggccctggaa ttggatatgt tacattcagt 3360
gattgtgcaa atcatataga aaagcttaca tttacttctt gcgattatgg tacaaacgta 3420
gtgccatatg aacaatctaa ttatcctaca gacggagtac catatggaca acatggttgt 3480
aatatagacg gagtaccgta tgaacaatcc ggttatcgta cagacggagt accgtatgaa 3540
caatccggtt atcgtacaga cggagtaccg tacgaacaat ctggtcatcg tacagatgga 3600
gtaccgtacg aacaatctgg ttatcgtaca gacggagtac catgcgaaca acatggttgt 3660
catacagacg gactaccaca catacaacat ggttgtcgta cagacggact accacacata 3720
caacatggtt gtcgtacaga cagatcaaga gatgaactac ttggttatgt gacaaaaacg 3780
attgatgtat tccctaatac agataaagta cgtatcgaca ttggagaaac cgaaggtact 3840
tttaaagtag aaagtgtaga actgatttgt atggaagag 3879
<210> 18
<211> 3768
<212> DNA
<213> 侧孢短芽孢杆菌
<400> 18
atgaatcaaa atcaaaatca gaatcaaaat aaaaatgaac tgcaaatcat agaaccttca 60
agcgattctt ttctttatag tcacaacaat tatccgtatg ccactgatcc aaatacagta 120
ttacaaggta ggaattacaa agagtggcta aacatgtgta caggtacaga cgattcacga 180
agtcccgaag ctgcttctac tgcaaaatca gctatttcag ttgcgattac tataagcacc 240
acaattcttg gcttactagg tgttccgttt gcatctcaaa tcggggcatt ttataacttc 300
gtattgaata cggtttggcc tcagggaaat aaccaatggg aagagttcat gagacatgta 360
gaagatctca taaacgaacg aatagctgat tatgcaagaa gtaaggcact tgcagaatta 420
gcgggtttag gtaataactt agatttatat agagaggctt ttgaagattg gagacgaaat 480
cctactagtc aacaagctaa aacccgtgta atagaaagat ttcgtatact agatggtctt 540
tttgaacaat atatgccatc atttgcagta caaggttttc aagtacaatt attaacagtg 600
tatgcatccg ctgcaaatat ccatttattt ttattaagag atagctctat ttacggtttg 660
gattggggat taagtcaaac taatgttaac gaaaattaca atcgccaaat aaggcacgcc 720
gcaacgtatg caaatcattg tacaacttgg tatcaaactg gtttacaaag attgcaaggt 780
accaatgcta ccagttgggt cgcttataat agatttagaa gggaaatgac attaacagta 840
ttagatatta gttcattatt ttcaaattat gattatcgta gttatccaac agaggtaagg 900
ggagagctta cgagagaaat ttatacggac ccagtaggta gaaactggca gaatagtgca 960
ccatcattcg ctcaaataga aaatctagca attagggcac caagaaccgt tacttggtta 1020
aattcaacaa gaatttctac agggaccttg cagggctgga gtggttctaa cagatattgg 1080
gcagctcaca tgcaaaactt ttcagaaacc aattcaggaa atatacgatt tgacggtcct 1140
ctatatgggt cgacggtagg tactattcat cgtactgatg attacgatat ggggaatcga 1200
gatatttaca ccattacttc agaagttgtt gcctcccttt gggcaactgg tcaaactgtg 1260
ttgggagtcg cttcggctag atttacttta agaaatcttt tcaataatct tacacaggcg 1320
ctggtgtatg agaacccaat aagttcaagt tttaataggt caactttaac tcatgaatta 1380
cctggagaaa actcagatag gccaacttct agcgactata gtcatagact atcgagtatc 1440
acaggttttc gagctggagc taatggaacg gtcccagtgt ttggttggac atctgcaact 1500
gttgatcgta acaatataat tgagcgaaac aaaataacac aattcccagg tgttaagtca 1560
cacactctca acaattgtca agtagttagg ggtactggat ttacaggagg agactggttg 1620
agaccaaata ataatggtac atttagacta actattactt cattctccag tcaatcttac 1680
cgaattcgct tacgttatgc tacttcagta gggaatactt ctttagttat atcttcttct 1740
gatggaggta tttcttccac aacaattccg cttacctcaa caataacatc actgccacaa 1800
actgtaccat accaggcttt tagggttgta gatttaccta ttacttttac aacacctact 1860
acccaaagaa attatacgtt tgatttccgt ctccaaaatc catcaaacgc aaatgtattc 1920
attgatagaa ttgaatttgt tccaattggg ggttctttgt ctgagtatga aaccaaacat 1980
cagctagaaa aagcaaggaa agcggtgaac gatttgttta ccaatgaatc gaaaaatgtg 2040
ttaaaaaaag acacgaccga ttatgatata gatcaagctg caaacttggt agaatgtgta 2100
tctgatgaat gtgcaaatgc taaaatgatc ctattagatg aagtaaaata tgcgaaacaa 2160
cttagcgaag cccgcaatct acttctaaat ggtaattttg ataacataga tagagatggt 2220
gagaatccat ggaaaacaag tcccaatgtt accatccaag agaataaccc catttttaaa 2280
ggccgctatc ttagtatgtc aggtgcgaac aatatcgagg caaccaatga gatatttccc 2340
acttatgcat accaaaaaat agatgaagca aaattaaaac cctatacgcg ttataaagtt 2400
cgagggtttg ttggaaatag taaagattta gagttgttgg ttacacggta tgatgaagaa 2460
gtagatgcaa ttttaaatgt accaaatgat ataccacatg ctccgccacc tttttgcggt 2520
gaatttgatc gatgcaaccc gcattcttat cctcctatga atccagaatg tcaccatgat 2580
gtaataaata acattgaaat atcctctcct tgccaacaca ataagatggt agataacgct 2640
gatatatctt atcgccatag ccataaaaaa catggcattt gtcatgaatc tcatcatttc 2700
gaattccata ttgatacagg gaaaatcgat ttggtcgaaa atttgggaat ttgggttata 2760
tttaaaatat gttccacaga tggttacgca acattagata atttggaagt tattgaagag 2820
cgtcctttag gagccgaatc attagaacgt gtgaaaagaa gagaaaagaa atggaaacat 2880
cacatggaac acaagtgttc agaaactaaa cttgcatatc atgctgcaaa acaagcgctg 2940
gtggggttat tcacaaacac tgaatatgat agattaaagt tcgaaacaac catatccaat 3000
attctttttg ctgattatct cgtgcagtca attccgtatg tatataataa atggttacca 3060
gatgttccag gtatgaattt cgagatctat acagaattaa aaaatctgta tacgggagct 3120
ttcaatttat atgatcagcg aaatattata aaaaatggag actttaatcg cgggctcatg 3180
cattggcatg cgacacctca tgcaagagta gagcaaatag ataataggtc tgtgctggtg 3240
cttccaaatt atgctgccaa tgtttcacaa gaggtttgtt tagaacacaa tcgtggttat 3300
gtattacgtg taacggcgaa aaaagaaggc cctggaattg gatatattac attcagtgat 3360
tgtgcaaata atatagaaaa gctgacattt acttcttgcg attatggtac aaacgaagtg 3420
ccgtatgagc aatctaatta tcctacagac ggagtttcat acggacacca tggttgtaat 3480
atagacagag taaggtacga agaatctggt tatcgtacag acggtgtacc gtacgaacaa 3540
tctggttatc gtgcagacgg agtatcgtac gaacaacatg gttgtcatac cgacggagta 3600
ccatacaaac aacatggttg tcgtacggac agatcaagag atgaacaact tggttacgtg 3660
acaaaaacga ttgatgtatt ccctgatact gataaagtac gtatcgacat tggagaaacc 3720
gaaggtacct ttaaagtaga aagtgtggaa ctgatttgta tggaagag 3768
<210> 19
<211> 951
<212> DNA
<213> 侧孢短芽孢杆菌
<400> 19
atgaaaaaat ttgcaagttt aattcttata agtgtgttcc ttttttcgag tacgcaattt 60
gttcatgcgt catccacaga tgttcaagaa agattacggg acttggcaag agaaaatgaa 120
gctggaaccc ttaatgaagc atggaatact aacttcaaac ccagtgatga acaacaattc 180
tcttatagtc caactgaagg tattgttttc ttaacaccac ctaaaaatgt tattggcgaa 240
agaagaattt cacagtataa agtaaataat gcatgggcta cattagaagg aagtccaacc 300
gaagtatcgg ggacaccttt atatgtggga aaaaacgtat tagataactc aaaaggaaca 360
agcgatcaag agctgttaac acccgagttt aactatacct atacggaaag cacttcaaat 420
acaacaactc atggattaaa attaggagtc aaaaccactg ctaccatgaa attcccgatt 480
gctcagggta gcatggaagc ttctactgaa tataactttc aagattcttc cactgatact 540
acaactaaaa cagtatcata taaaagccca tcacaaaaga ttaaagtacc agcaggtaaa 600
acctttagag ttttagcata cctaaatact ggatctattt caggtgaagc taacctttac 660
gcaaatgttg ggggtatagc ttggggggtt ttaccaggtt atcccaatgg cggaggagta 720
aatataggtg ctgtacttac caaatgccaa caaaaaggat ggggagattt cagaaacttt 780
caacctagtg gaagagatgt aatcgttaaa ggccaaggta ctttcaaatc taattatgga 840
acggacttca ttttaaaaat tgaagacatc acagattcaa agttacgaaa caataacggg 900
agtggaactg tcgttcaaga gattaaagtt ccactaatta gaactgaaat a 951
<210> 20
<211> 3462
<212> DNA
<213> 苏云金芽孢杆菌
<400> 20
gtgaattttt tatttttagt taattatgaa aaaaataagt ttaaatataa tatacaagga 60
gacttgaata tgaatcaaaa aaactatgat attataggtt cttcgacaaa cggcacaacg 120
aaattacctg aagattataa cattataatt agtcctgatg cagccccaga ggctgttact 180
attgcgattt caattacagg agaagtactg tctctttttg gtgttccagg tgcaacatta 240
ggaagtactc ttcttaatac acttgttgat aaattatggc caaccaatac aaatactgta 300
tggggtacat ttacagagga aactgcaaaa cttataaatg aagtatataa tccatcagat 360
ccagtagtaa aagatgcaga tgctcgacta acatcgttac atgaatcgtt aaaattatat 420
caattagcct ttggaaattg gtttaaatca caagataatt caaaactcaa agaagaggta 480
cgacgccagt ttgatattac tcataataga tttgtaacta gtatgccttt ttttaaggta 540
tcggactacg aaattagatt gttaacaaat tatgctcaag ctgctaatct tcatttaact 600
tttttaagag atgcgtccat ttatgggctt gattggggtt tcagtgacga gcatagtaat 660
gatttgtatg aacaacaaaa gaatcgtaca ggagaataca cagatcattg tgtaaagtgg 720
tataacgcag gattagaaaa attaaaagga aatttaactg gggaaaattg gtacacttat 780
aatagatttc gtagagaaat gacgttaatg gtgttagacg tagttgcatt atttccaaac 840
tatgatacac gaatgtaccc gatcgcaacg tcatcagaac ttacaagaat gatttataca 900
gatccaatcg cttatacaca aagcgatcca tggtacaaga taacatctct ttctttttcg 960
aatattgaaa acagcgcgat tccaagtcct tctttcttca ggtggctaaa atccgtttca 1020
attaatagcc agtggtgggg cagtggtcct aatcaaacct actattgggt tggacatgaa 1080
ttggtatatt ctaattcaaa ttataatcaa tcacttaagg ttaaatacgg ggatcccaat 1140
tcttatattg agccccctga ttctttcagt ttttcttcta cggatgttta cagaaccatc 1200
tctgtcgtta gaaattcaat tagtaattat atagtaagtg aagttcaatt caattcaatt 1260
agtaatacaa atcaaattag tgaagaaatt tataaacatc aatcaaattg gaatagaaga 1320
gaaaccaaag attcaattac tgaactatcc ttagctgcta atcccccaac aacatttgga 1380
aacgtagcag aatacagtca tagattagca tatatttcag aggcatacca aagtaacaac 1440
ccatcaaaat acccagccta cattcctgta ttcggttgga cgcatacaag cgtacgttac 1500
gataataaaa ttttcccgga caaaatcact caaattccag ctgttaaaag ttcctcagct 1560
gaaggtggaa catggaaaaa tatagcgaaa ggtcctggat ttactggagg cgatgtgaca 1620
acagctgttt cgccagcatt tataacagat gtaataaaaa tacacgttac tctagatcca 1680
aattcacttt cacaaaaata tcgtgcacga cttcgctacg cttccaatgc atatgtagca 1740
gctacattgt atacaaattc aagtagtaat tataattttg aacttacaaa aggtacaacg 1800
gaacagttta caacatataa ttcataccag tatgtagata ttccaggttc aatacaattt 1860
aatactactt ctgatacagt gtctgtttat ttgcatatgg attcaacaac taatgcaaac 1920
gttcatgtag atagaattga attcattcca gtagatgaaa attacgataa cagagtaaca 1980
ctagaaaaag cacagaaagc cgtgaatgcc ttgtttacag cgggaagaca tgcactccaa 2040
acagatgtga cagattttaa agtagatcag gtttcaattt tagtggattg tgtatcaggg 2100
gaattatatc ccaatgagaa acgcgaacta ctcagtttag tcaaatacgc aaaacgtttg 2160
agttattccc gtaatttact cctagatcca acattcgatt ctattaattc atctgaggag 2220
aatggctggc acggaagtaa tggtattgca attggcaatg ggaactttgt attcaaagga 2280
aactatttaa ttttctcagg taccaatgat acacaatacc caacgtatct ctatcaaaaa 2340
attgatgaat ccaagctcaa agaatataca cgctataaac tgagaggatt tatcgagagt 2400
agtcaagatt tagaagcata tgtgattcgc tatgatgcaa aatatgaaac attggatgta 2460
tccaataatc tatacccaga tatttctcct gtaaatgcat gcggagaacc caatcgttgt 2520
gcggcactac catacctgga tgaaaatccg aggttagaat gtagttcgat acaagatggc 2580
attttatctg attcgcattc attttctctc aatatagata caggttctat tgattccaat 2640
gagaacgtag gaatttgggt gttgtttaaa atttccacac cggaagggta tgcgaaattt 2700
ggaaacctag aagtgattga agatggccca gtcattggag aagcattagc ccgtgtgaaa 2760
cgtcaagaaa cgaagtggag aaacaagttg acacaactgc gaacggaaac acaagcgatt 2820
tatacacgcg caaaacaagc cattgataat ttattcacaa atgcacagga ctctcactta 2880
aaaataggtg ctacattcgc gtcaattgtg gctgcgcgaa agattgtcca atccatacgt 2940
gaagcgtata tgccatggtt atctatcgtc ccaggtgtaa attaccctat tttcacagag 3000
ttgaatgaga gagtacagca agcatttcaa ttatatgatg tacggaatgt cgtgcgtaat 3060
ggccgattcc tgaatggagt atcggattgg attgtgacat ctgatgtaac ggtacaagaa 3120
gaaaatggga acaatgtatt agttctttcc aattgggatg cgcaagtatt acaatgtctg 3180
aagctctatc aagatcgcgg atatatcttg cgtgtaacgg cacgtaaaga aggattggga 3240
gaaggatata ttacaattac ggatgaagaa gggtatacag atcaattgac atttggcaca 3300
tgtgaggaga tagatgcatc taacacgttc gtatccacag gttatattac aaaagaactg 3360
gaattcttcc cagatacaga gaaagtgcgt atagaagtag gagaaacaga aggaaccttc 3420
cgggtagaaa gtgtagaatt attcttgatg gaagaacact gt 3462
<210> 21
<211> 3618
<212> DNA
<213> 苏云金芽孢杆菌
<400> 21
atgcggttaa aaaaattact tgtatgtaat ataagaattg gaggaacaaa tatgaatttg 60
ggaaactata atgaattcga tatcatagat attactgaaa acaatcagac taaaacatca 120
cgatataata atgtgaatag acaagagaat ccatctaata tgattatttc aaatccttct 180
tctaactatc ctctagcaaa caatccaaat acacccttcc aaaatataaa ttataaagat 240
tttttgaata tgaatgagga gattgcaccg tatgcaagtt cgaaagatgt aatttttagt 300
tcaatgaata tcattagaac cttcatgggt tttgcaggac atgggactgc tggaggtatt 360
gttgcattat ttacggaagt attaagatta ctatggccta ataagcaaga tgagctttgg 420
gaatcgttta tgaaagaagt agagaaactt attgaacaag aaataacaga tgcggtagta 480
agtaaagctt tggcagaatt agagggttta agaaacgctt tgcaaggata tacagatgca 540
ctggaagcat ggcaaaataa tcgtagtgat aaacttaagc aattactagt gtatgataga 600
tttgtttcta cagaaaattt atttaaattt gcaatgccgt cttttagagt gggaggtttt 660
gaagttccat tattaacagt atatgcacaa gccgcaaatc ttcacttatt attattaaaa 720
aattccgaat tattcggggc agaatgggga atgcagcaat acgaaataga cctattttat 780
aatgaacaaa aggattacgt agtagaatat acagatcatt gtgttaaatg gtatactgaa 840
gggttaaata ggttgaagaa tgcaagcgga gtaaaaggta aggtatggga ggaatataat 900
cgttttcgca gagaaatgac gattatggtg ttagatcttc ttccattatt tccaatctat 960
gatgtacgca catatcctac ggaaacagta acagagttga caagacaaat tttcacagat 1020
ccaataggtc ttagaggaat taatgagtcg aaatatcctg attggtatgg agctgcaagt 1080
gatagtttca gtcttataga aaatagggca gtaccacaac ctagcttatt tcaatggtta 1140
actgaattta aagtatatac taaatatgtt gaaccgaatg ataagcttac aattttggct 1200
ggacacagtg taactactca atatactagc tattataaaa agagcacatt tacttatgga 1260
gatacttcaa gtgctaattc atctagaact tttgacctac ttgctaaaga tgtatatcag 1320
gttgattctg tagctgcagc aagtaaaagt gctacttggt atttggctgt tcctgaaatg 1380
cgattatata gcattaatac taacaatata ttatctgaag attatttttc tttgagtact 1440
aatataccat ccagtaagat gagacgtatg tattctagtg aggaattacc gataggaatc 1500
tcggatacac ctatttatgg agatcttgag gaatatagtc ataggttaag ttttatttct 1560
gaaattatgc ataactctgg aagtgtaaca ggttcaaata acatcaaagg aataattcca 1620
gtattaggat ggacacatac aagtgtatct cctgaaaatt atattcacag ggataaaatt 1680
tcacaattat atgctgttaa agcatacacc actagtaatg tttctgttgt aggaggacct 1740
ggatttttag gaggaaatat aattaaaggt aataatgatc ctgctagcta taccggaagc 1800
gtgagctggg caattagatt ggatggttca acagtaagtc gattccgtct tagaattccc 1860
tatgctgctg aaacagatgg cacattttct attactgttc gagacgattt aggccctttt 1920
actataaaga aggactttat agcaacaatg aaaccaggag atcctttatc atatggtaaa 1980
tttgaatatt tagaatttga acaaacaatg agtcttaata ataagcatgg tcaatttttc 2040
gttcatacag aaaatttaaa agatagaaat tctagtgtat attggaatag agttgaaatt 2100
atcccggtgg atgaaaatta cgataacaga gtaagattag aaaaagcaca gaaagccgtg 2160
aatgctttgt ttacagcggg aagacatgca ctccaaacaa atgtgacgga ttacaaagtg 2220
gatcaggttt caatcttagt ggattctgta tcaggggaat tatatccaaa tgagaaacgc 2280
gaactacaaa gtttagttaa atatgcaaaa cgtttgagct attcccgtaa tttacttcta 2340
gatccaacat tcgattctat taattcatct gaggagaatg gctggtacgg aagtaatggt 2400
attgcaattg gaaatgggaa ctttgtattc aaaggaaact atttaaattt ctcaggtacc 2460
aatgatacac aatacccaac gtatctctat caaaaaattg atgaatccaa gctcaaagaa 2520
tatacacgct ataaactgag aggatttatc gagagtagtc aagatttaga agcatatgtg 2580
gttcgctatg atgcaaaaca tgaaacattg gatgtatcca ataatctatt cccagatatt 2640
tctcctgtga atgcatgcgg agaacccaat cgttgtgcgg cactaccata cctggataaa 2700
aatccgaggt tagaatgtag tttgatacaa gatggtattt tatctgattc gcattcattt 2760
tctctcaata tagatacagg ttctattgat tccactgaga acgtaggaat ttgggtgttg 2820
tttaaaattt ccacaccgga agggtatgcg aaatttggaa acctagaagt gattgaatat 2880
ggcccagtca ttggagaagc attagcccgt gtgaaacgtc aagaaacgaa gtggagaaac 2940
aagttgacac aactgcgaac ggaaacacaa gcgatttata cacgagcaaa acaagccatt 3000
gataatttat tcacaaatac acaggactct tacctaaaaa taggtgctac attcgcgtca 3060
attgtggctg cacgaaagat tgtccaatcc atacgtgaag cgtatatgtc atggttatct 3120
atcgtcccag gtgtaaatta tcctattttc acagagttga atgagagagt acagcgagca 3180
tttcaattat atgatgtacg gaatgtcgtg cgtaatggcc gattcctgag tggagtatca 3240
gattggattg tgacatctga tgtaaaggta caagaagaaa atgggaacaa tgtattagtt 3300
ctttccaatt gggatgcaca agtattacaa tgtctgaagc tctatcaaga tcgcggatat 3360
atcttgcgtg taacggcacg taaggaagga ctcggagaag gatatattac aattacggat 3420
gaagaagggc atacagatca attgacattt ggcacatgtg aggaaataga tgcatctaac 3480
acgttcgtat ccacaggtta tattacaaaa gaactggaat tcttcccaga tacagagaaa 3540
gtgcgcatag aaattggaga aacagaggga atattcaagg tggaaagtgt agaattattt 3600
ttgatggaag atctatgt 3618
<210> 22
<211> 2316
<212> DNA
<213> 苏云金芽孢杆菌
<400> 22
atggtgaatg aaaatatgga tatgtataat aacaacggta gtatgaacgg aaatccagat 60
atgtacaata aaaacggaag tatgaacgga aatacggatg tgtataataa caacggtagt 120
atgaacggaa atccagatat gtacaataac aacggaagca tgaacggaaa tacagatgtg 180
tataataaca acggaagcat gaacggaaat ccagatgtgt ataataaaaa cggaagcatg 240
gacggaaatc cagatatgta caataacaac ggaagcatga acggaaatac agatgtgtat 300
aataaaaacg gaagcatgaa cggaaatcca gatatgtaca ataacaacgg aagtatgaac 360
ggaaatacgg atgtgtacaa taacaacgga agtatgaacg gaaatacgga taatcaagtg 420
ccggcttata acattctttc tgcggaaaac ccttctaata ttttagaaag tgatactaga 480
tgcacactaa atgttaaaaa tgtgcaagat gaggctatct gtacaggtag taatttaacc 540
aacgaaatag gtccacttgt tgttcccatt gcttttactc ctattattct aacgcctgca 600
cttattgaag taggtaaatg gttaggagtt caaattggta aatgggctct aagtacagct 660
ttaaaagaat taaaatcttt tctctttcca aattctgatc cccaaaggga aatggagaaa 720
ttacgcatag aattagaaaa ttcatttaat aagaaattaa cagaagataa attgaatttt 780
ttaactgccg cgtatactgg ttttaataat ttatctaatt cttttatttc tgcaaccgag 840
cgtgtaaaag cagcagaaat tacattagct acagctcctt ctcaagaaaa tcaagatatt 900
ttagatgaag ctagaacatt agcaagagac tattttgtga gtttacactc acaaatgata 960
gtgtggcttc cccagtttga aattagtgga tatgaagaaa tttccttacc attatttact 1020
cagatgtgca ctttacatct cactcatcta aaagatggag tattaatggg gcagaattgg 1080
gggctttcta cagatgatat taaacatttt aaaggtgaat tttacagatt aagcaatgat 1140
tacacttcta gagctttcga ttcatttcat agaggtttta atcgtttacg aacacaacaa 1200
ggaacagctg gagtcataaa atttagaaca gccatgaatg catatgcttt tgacaatata 1260
tataaatggt cattgttgcg ttatgagggt attaatccta ggataacaag aagtttatgg 1320
cattatattg gatataattc atctttagga tctaatgatt ttaatacact atacaaactt 1380
atggtgggta taccgcatga aagatttaga acagttgcaa taggatatcg tgctaaaaca 1440
ggtgaggatt ggaaagttac aggggctaaa tcaacttttt attctggtgg tggtgaatgg 1500
gttggaaacg tctccaaagc aacaagaatc cctgtttaca ctactaaaac ggattggagg 1560
caatttgaaa gaagaataca tggcagatta ggaactgagc aatatactag atggcatctt 1620
acaattcaag atacgaatat cattggtaat tcatatttaa ccggtttacc ctttgatatt 1680
tcatatcctg attattttat ccgaacaatt tcagcaaaac cagaagccta ccctatttat 1740
aaatcgctta gtctggggga taatccagga tacgtagtag acaatcctgg aaataacctt 1800
attataggtt tttctcctga taatttaaaa acatttatga ctgatggaaa cagatatcat 1860
tcaatagaat caggatatcc aacaaaccca tcttgtacta taccagcggt actttataat 1920
agtgtaagta acccattcca agcttatttt aatgatgaat taggtaatgg ttcagatggg 1980
agtataactt taatccgtcg aggtggtgca cattatcttg ttgattcaag atctgcttct 2040
tatgacagaa gctttcgtct tataattaga attcaggcag ggagttctgc attcaaggta 2100
acagtaagat caaggcacac atctgagagc tttgaattaa atttcacact tctttcagat 2160
caagatatta attattatta tgattatata tcccaacctt ttaatctaag ctctacttac 2220
tattatatag atgtagaacg tgttgttagt gatgacataa gagcgttaac ttttaatcaa 2280
atgattatag ttccaactac agaattccag atatta 2316
<210> 23
<211> 867
<212> DNA
<213> 苏云金芽孢杆菌
<400> 23
atggcaattt acgatatagc agcagatttg ttcgatctta ccagatggta cgccgaacaa 60
aattataatg caaatccaac aacatttaga ggggctaaag tatatgatcg aatcgtatca 120
gatgttcaat ctataccgga aaaagtagat tttaatttga taccaggctt agcttatacg 180
gtgaaaaatg aaatcgtaaa tgatactaat acagaacaat ctatgagtac aaaactcatg 240
catacattaa ttgaatcaaa ttctgttaca accacaaaag gatataaaat tggaagtagt 300
atcaaaaata cgtttagcgt aaacattgag ggaagttttt ttgttggtgg tgggtctaca 360
gagcattcca ttgaagtatc agtaagtggg gaatataatc atagttcttc agaaactaaa 420
acaaatacct cacagaaaac atgggaatat aacagcccta ttcttgtccc agcaaaaaca 480
aaagttacag caactttaga tatttatgca ggaccagttg tagtcccagt aactttaaaa 540
agtacagtta ctggaacggg tattgtgaat aattttccta acgtattaac gagtttaagt 600
tacattgata gaaacaataa gttgtggaca gactctcttc caactgcttt gttatatgat 660
tatcgaaatc agtggccagg aagtcagtct atttatgttg ggaagaatgg gggaggtgta 720
caggtagaag gtaaagctga aatacaatta gaactaggtc tatactccat tgcaaccttc 780
gactctcagc cactgtcagg gaacacaaca ggtaaagaag cagtgtattc taaggctata 840
ctccgagatg gatctattat tgatatt 867
<210> 24
<211> 318
<212> DNA
<213> 苏云金芽孢杆菌
<400> 24
taaattcaat tatgattcgt aattaaaatg tgaaaattaa caatctccga atttcatgtt 60
aatttatggg tgtcaaataa aattatataa ggcctactta tattttcgag attactatat 120
gaggcctaaa atatttaaca aattgctatg tcttgcatat aaacaaattt aagtatggtg 180
aatttctcaa gatatatgta taaaaatgat aattatatcc tttgttgttt tttgtgaata 240
gatgaatgct aactattctt acctatgaaa aatagattaa tttgcctata aagtattttt 300
taaaaggagg aatgtatt 318
<210> 25
<211> 3999
<212> DNA
<213> 苏云金芽孢杆菌
<400> 25
ttggcacaat taaatgaaat ttatccaagt tattacaatg ttttagcata tccccctcta 60
attctcgacg ataagagcct atatgatcag tatacagagt ggaagaaaaa aattgataag 120
acttggaaac aatatgacaa agacttttta ccaaagcctt taatggattt aggaaaatct 180
ctggcagagg cctataaggg tgatcctgat ggttaccttc atatcgcaaa cacagcaata 240
agaatagctt tcttattgat accaggaggg caaactgctg cttttggtgt aaatcttgtg 300
ctgaataaag caatagggat cttttatcct cctcaaaata aatctctatt tgatcaaatt 360
aaggacgctg tatccaacct ggtggatcaa aaattgatag accaagaaat ctctggagta 420
ctgattaaac ttaacagcct acagcagccc ctatcacgtt tcagcaattc catacagcga 480
gctgttggaa aaccacagga cttcgacgac caaactacat catccaacgc aattattctt 540
gatgagacac aggactgcag taaggacgat tcgtgttctt gttccaatac tcaacctcgt 600
ccttctgatg cgcctctttg taccccatgt atttgtcgta tgaaggaagt tcaacaaaca 660
ttcaacaact caagtactga tgttaaccga gcattaactg acatgaaaac aacgttaaag 720
gacgtagtgg gtgcggatca actgagaagc tacatgcaaa tatatttacc gttatatgtg 780
acggctgcta caatggagtt acaaatgtat aaaacctata ttgactttac acaaaaattc 840
gatttcgatg taactggcac gacaaaggaa catgtaaacg agttacgtca aaaaatcaaa 900
acgcatagtg agtacatcat gggcttattt aagaaatctt taccagaaat tagtaataat 960
acaaaagaac aactaaatgc atatattaaa tatacacgta atataacttt gaatgctctt 1020
gatatggtaa gtacatggaa atttttagac cctgttgact accctacgac tgctacattc 1080
aatccgacta gaattatatt taacgatctt gcaggacctg ttgagtgtct gaatagcact 1140
caggacagca ataaactaca ttttaatttc tttgatatga acggacagtc tatgcctaat 1200
aatgatattt tcaattattt ttatagaggt atgcaagtaa aaggtctgca gatccaaacc 1260
tatactagct ctgacaccaa aaatccacag cattttcctg taggattcct atcctcttat 1320
tatggtagta acggtgattt tccatttgac aagagagtag atcctaacaa atttacaggg 1380
ggaagcaaat ccgtcaaact gggagacgat gtatatgaga gccgttcagc tttaagtgta 1440
ataaatgcag tgagtaatca gctacaagtt tttctaaact atattgatac agaagatttg 1500
tattttgacc aatctgtatc ccctggtggc actgcatgtg ggtcaggtaa ttccacaatt 1560
tggccagacc aaaaaattca agctatatac cctatacaac ctgataattc tcaaacatat 1620
ccaagttatt attcaacaag taaaatagga tttgttacta cacttgtccc taatgatacc 1680
actccatgga tcacttttac cgataacggc aataacagca tttatacgtt ttctgcagaa 1740
aatacacgaa ctcttacagg ttcagcggga ccggttcgtg aatttataac tggttcagct 1800
cctcttggac tgtctcctgg aggcggtgca caatattcta ttaatactag tgatgctcct 1860
agcggagatt atcaggttcg cgttcatgta gccacacctg gctctggtgg gtcccttgct 1920
atctcagttg atggaaaaac gcaaacttta caactaccgg atacaaatgt gaacgataca 1980
aaccatatag cgggattcgc gggaacatat acgcttgctc ctgcaaccca agtagacgct 2040
gcaaccctta aaccaaaggc acctactgaa aatattttcc cagtgcgtca aacatcttct 2100
ctacctgtga gcataaccaa taattcttca acagttatta atatagaccg cattgaattt 2160
gttcctgttt ctgctcctgc tcctgatcct agtcctgact ctggtaagcc aatacacaaa 2220
tcagtgccta agacagtgac acaactaagc acaacgaaag agatttggtc atctactagc 2280
gagtatgcta caactatatc ttttacaggg aatgtttata acgatgcttc cattacattt 2340
cagttattaa gttcaggtca ggtagtgaaa gaatttccat ttaccggaaa cggggtcgcg 2400
agtaaaccag gttttcatgg cagttcaccg tcctgctatg acacgcctta tccattttcc 2460
caaccggacc tatcagtgcc taaatataat aaattgcagg tggtaatgaa gagcgatggt 2520
tactcaaaac cttgtgatct tggcgactca ttccctaata cttttgatgc ggaaatagac 2580
ataaagttta atttgagtga taccgcagac ttagcacaaa tcactgcaca agtgcaggga 2640
ttattcacat cttcttcatc tacagaatta tctccaaacg tttccggcta tcaaattgat 2700
caaatcgcac tgaaagtgaa tgcactatcc gatgaagtat tttgtaaaga aaaaatagta 2760
ttacgcaaat tagtcaacaa agccaagcaa ttcatgaaga cacgtaatct gctgataggc 2820
ggggattttg aaatacttga taaatgggca ttaggaacac aagctactat aaaagataat 2880
tcatctttat ttaaagggaa tcacttattt ttacaaccga ctaatggcat atcttcatct 2940
tatgcttatc aaaaaataga tgaatccaag ttaaaaccct acacacgcta taacgtttct 3000
ggttttgtag cgcaaagtga acacttagaa attgtcgttt ctcgctatgg gaaagaaatc 3060
gataaaatat taaatgtccc atatgaagaa gcattaccgg tttcttctgg gaatcagtcc 3120
acttgttgca aaccatcttc ttgctcatgt tcagcttgta ctggtggacc acatccacat 3180
ttctttagct atagcattga tgtcggtaag ctatatccag acttaaatcc aggaatagaa 3240
tttggactgc gtcttgcaca cccaagtgga tatgcaaaag tcggcaatct cgaaattgta 3300
gaagaacgcc ctcttacaaa cacagaaatt cgaaaaatcc aaagaaaaga agagaaatgg 3360
aaaaaagcat gggatacaga acgggcagaa attaatgcca tccttcagcc agtcattaac 3420
cagatcaatg ctttctatac aaatggagat tggaacggtt ctattcttcc tcatgtcaca 3480
tatcaagacc tatataatat cgtattacca gaattatcaa aattaagaca ttggtttatg 3540
aaagatcgtc caggtgaaca ctatactatc ctccaacaat tcaagcaagc tttagaacgc 3600
gtattcaatc aattagaaga aagaaactta atccacaatg gtagttttac aaatggatta 3660
gcaaattggc tggtagacgg agatacccaa ataactacct tagaaaatgg aaatctcgca 3720
ttacaactct cagactggga tgcaagcgca tcacaatcca ttgatatctc ggactttgat 3780
gaagataaag aatatacagt tcgcgtatat gcaaaaggaa aaggaaccat tagaactgta 3840
aactgtgaaa atgagcccct atcctttaat acaaacacat tcacaatcct agaacaacga 3900
ttatatttcg acaacccatc cgttctcctg cacatacaat cagaaggttc tgaattcgtc 3960
ataggcagtg tggaactcat tgaattgtca gacgacgaa 3999
<210> 26
<211> 1134
<212> DNA
<213> 苏云金芽孢杆菌
<400> 26
ttgtattgta atacaatatt gcgaaaacgt tataaaaagt tagctacgat tataccgctt 60
acaagtatgt cagccgttgc gattgcacct gctacgtctt ttgcagttga aacacaaaaa 120
gcagatgttt catcacaaga agggccgatt caaggttacc agatggaaaa tggaaaaatt 180
actcctgtgt acaaaaataa acttacccag tttaatacgg cggatgatat cgatcctggc 240
cttccactac tcccagagaa tccatataat ccaattcctg atcatggaac tgcatatgtt 300
gaatcaactg atataggaga tactgtatat ttcaaaccat ttgaaccccc taaaaataat 360
gtattagagt taggtgactg tgatgataat acttatcagt ggtccgtatt tgtagattca 420
cagaaatata aaagtgtagg atactttgtt caaaaacaag ccgatggtca aattagagtt 480
ggatattata atccagaaga tttatctctg attacagatt caaaccatgc tttcgcagga 540
gtgccaggtt tcaaactgac agcagaagag aaagctgaga tgcaacgaga tttaaatcga 600
gaatatggcg atatatggga tggcacaagt aaactaaaac gagaaacaaa ctataaactt 660
ctgccaaatg cctcaggtct acaggatgac gcatcgggat ttggttataa tcaaacatta 720
acttcgggtg tatcaactac aaatatgttt ggaatagcga caacagttgg gtggaaaatg 780
gggataaaag tatcggttgt tcctcttgtt gcagacgtta cgtcagagat tagtgcaagt 840
ttaacagcta gttatcagca tactgtaaac gttacaaacc aaacgagttc gcaagtgaaa 900
tttgacgtat caagagtaga taaccctgac tataagtata atgactatgc ggcagctgta 960
tacaaaatat acacagacta tacattagaa ccgggtaaag gattatctcg ttttttagca 1020
aagcaagatc ttaaagatcc tgtgcgtaca gctgcattag caaatacgaa ttatgcatat 1080
gaaggttcaa aatactactt tacagtaaca cctggatcac acaagaaaat tgtg 1134
<210> 27
<211> 1149
<212> DNA
<213> 苏云金芽孢杆菌
<400> 27
atggattttt taaattatta taataaatta aaaaatgaat tggatgatgt aaattctaaa 60
aaatattctt tagaatatac atcagatgga ttaatggttc aacctactga tgatccatta 120
aacacaatgc ctttgcctga tagacctgta ttatctggaa acccaaatga ccctatccct 180
tcagaaggaa caacacgtac agatattcaa aaacaaaatc cacccttttt tacatttaaa 240
gtagtagcta aattagctta ttctggaaaa ggtgaaaatt gtcaaaaggc tcgtgcagca 300
tcagtttatg gtgcagttct tgaacttgaa aaagttaaac aattaccaga atattctaat 360
gtatatttat actcggaaac aggaataaaa acagatcgta gtaatataag atacaacacg 420
gacggtataa tacaattttt gaatcctagt tttataaaca cattttcttc aaatcctata 480
aaatatgggg atacagtagg ttatatttct tatccatatg atacattaaa atttccttct 540
acaacacaat tagaacgttt ggtgtacttt aatttactag atagtaatat tcttgataaa 600
cacattggtt ttgattggtc aaaaagtgtt acaaatggaa cagaagatac agaaatgtgg 660
actcatagtt ccactgttgg cgctgaatta aatcttaaag atatactaca gatcaatgca 720
agttatgagc atacattttc aacgtctcat atggagaaaa aagagaatac tgtatcaaaa 780
actgctcatt ttaatagtcc tttacctcct tataactatg ctacatgggt agcagctata 840
tatcaattat ccattcgata tcaacgtaca aatgcacaac cgatactaga tacgataaat 900
gctgttaatt caggattaac agcatctgaa acagatattt atctgaaggc attatacggt 960
gctgggaaga atggtaagcc tgctgtgggt gatccatcaa tattacacaa gttaagtaat 1020
gtcatagaag atgcttatga atatttatat tattcggata ctctttattt tactcaaact 1080
ccttctggaa acagcccaac accaaattct ccaaatcgca ttcaatttat tgccacagat 1140
cctcaaagt 1149
<210> 28
<211> 651
<212> PRT
<213> 苏云金芽孢杆菌
<400> 28
Met Glu Ile Lys Ile Gly Ser Gly Gly Thr Tyr Met Asn Pro Tyr Asn
1 5 10 15
Asn Glu Ser Tyr Glu Ile Ile Asp Leu Asn Thr Ser Pro Tyr Pro Ser
20 25 30
Asn Arg Asn Asn Ser Arg Tyr Pro Tyr Ala Asn Ala Cys Gly Phe Pro
35 40 45
Glu Asn Val Asp Trp Thr Ala Gly Ala Ser Ala Met Ile Ile Val Ala
50 55 60
Gly Thr Leu Leu Ser Ala Ile Gly Ser Gly Gly Val Gly Ile Val Ala
65 70 75 80
Ala Gly Ile Ile Ser Val Gly Thr Leu Phe Pro Phe Phe Trp Pro Gln
85 90 95
Asp Lys Pro Thr Ala Gln Val Trp Lys Asp Phe Ile Lys Gln Gly Asp
100 105 110
Thr Ile Thr Asn Lys Thr Ile Ser Ala Ala Val Glu Ser Leu Val Leu
115 120 125
Ala Glu Leu Asn Gly Leu Lys Ser Ile Leu Asp Val Tyr Thr Asp Ala
130 135 140
Leu Glu Leu Trp Lys Lys Asp Lys Asn Asn Ile Val Asn Arg Asp Asn
145 150 155 160
Val Lys Ser Ile Phe Thr Asn Leu His Leu Gln Phe Val Ala Ala Met
165 170 175
Pro Lys Phe Ala Thr Asn Gly Tyr Glu Val Ile Leu Leu Ser Thr Tyr
180 185 190
Thr Ala Ala Ala Leu Leu His Ile Thr Phe Leu His Glu Ala Leu Gln
195 200 205
Tyr Ala Asn Glu Trp Asn Leu Ala Arg Ser Glu Gly Thr Phe Tyr Arg
210 215 220
Gly Gln Leu Ile Gln Ala Ile Glu Asn Tyr Ile Asn Tyr Cys Glu Lys
225 230 235 240
Trp Tyr Arg Glu Gly Leu Glu Ile Leu Lys Asn Ser Thr Trp Asp Ile
245 250 255
Tyr Ala Ala Tyr Gln Asn Glu Tyr Thr Leu Ser Ile Leu Asn Val Ile
260 265 270
Ser Ile Phe Pro Arg Phe Asp Ile Arg Asn Phe Pro Thr Asn Ile Ala
275 280 285
Thr Arg Leu Glu Ser Thr Gln Lys Leu Tyr Thr Thr Thr Pro Asn Met
290 295 300
Lys Ala Leu Lys Thr Asn Asn Ser Ile Asp Tyr Ile Lys Asp Lys Leu
305 310 315 320
Ile Pro Pro Leu Asp Leu Phe Lys Lys Leu Lys Ser Leu Thr Phe Tyr
325 330 335
Thr Phe Leu Asp Ser Asn Asn Gln Tyr Asp His Leu Gln Gly Ile Val
340 345 350
Asn Asn Ser Tyr Tyr Thr Asn Ile Ser Thr Asn Lys Ile Phe Ser Ser
355 360 365
Gly Thr Thr Glu Gly Ser Ser Tyr Gln Leu Gly Leu Ala Ser Asp Gln
370 375 380
Val Ile Tyr Tyr Thr Asp Ile Phe His His Leu Asn Gln Ser Asn Phe
385 390 395 400
Lys Asp Gly Ser Leu Gly Ile Lys Ile Ile Asn Phe Asn Ile Ile Asn
405 410 415
Lys Tyr Asn Glu Val Ser Gln Lys Ser Tyr Asp Ser Asn Ala Thr Ser
420 425 430
Asn Leu Ile Leu Glu Val Ile Leu Pro Phe Leu Lys Thr Thr Glu Lys
435 440 445
Asp Tyr Lys Tyr Ile Leu Ser Tyr Ile Thr Ile Thr Pro Gln Gln Ile
450 455 460
Val Gly Cys Leu Ser Pro Ser Tyr Ile Tyr Gly Phe Ile Trp Thr His
465 470 475 480
Ser Ser Val Asn Leu Asn Asn Thr Ile His Tyr Thr Asn Lys Asn Asn
485 490 495
Phe Ser Gln Ile Thr Gln Ile Ser Ala Val Lys Ala Tyr Leu Lys Lys
500 505 510
Asp Arg Val Ser Val Ile Glu Gly Pro Gly His Thr Gly Gly Asp Leu
515 520 525
Val Lys Phe Thr Gln Trp Asp Asp Ser Ile Ser Thr His Tyr Gln Phe
530 535 540
Thr Ser Ser Gly Glu Tyr Lys Ile Arg Val Arg Tyr Ala Ser Thr Ala
545 550 555 560
Gln Val Asn Gln Thr Ser Gly Leu Ser Met Thr Ile Tyr His Lys Gly
565 570 575
Asn Pro Thr Glu Thr Trp Asp Leu Asn Ile Asn Asn Lys Ser Asp Thr
580 585 590
Ile Leu Asn Leu Asn Glu Pro Lys Tyr Asn His Phe Gln Tyr Thr Glu
595 600 605
Phe Pro Asn Lys Thr Leu Ile Ile Asn Lys Asp Pro Asn Ser Pro Tyr
610 615 620
Leu Glu Leu Arg Ile Asp Leu Ser Tyr Lys Gly Asn Thr Ala Thr Thr
625 630 635 640
Leu Ile Asp Lys Ile Glu Phe Ile Pro Val Ser
645 650
<210> 29
<211> 506
<212> PRT
<213> 苏云金芽孢杆菌
<400> 29
Met Asn Gly Asn Gly Lys His Asp Asn Trp Asn His Asn Gln Gln Ile
1 5 10 15
Ser Asn Val Gln Met Asn His Asn His Gly Arg Ser Tyr Asp Cys Ser
20 25 30
Cys Gln Gln Asn Gln Tyr Gly Tyr Glu Gln Gln Lys Gln Gln Tyr Glu
35 40 45
Gln Asn Asn Ser Gln Tyr Met Gln Asn Asn Leu Gly Asn Glu Asn Arg
50 55 60
Asn Gly Leu Tyr Pro Tyr Gln Glu Asn Gln Tyr Glu Gln Asn Lys Asn
65 70 75 80
Tyr Tyr Ala Ser Asn Asn Leu Thr Tyr Asn Gln Ser Asp Leu Tyr Asn
85 90 95
Ser Asn Pro Gln Asn Met Tyr Lys His Gln Thr Tyr Ser Asn Asp Phe
100 105 110
Tyr Cys Ser Pro Ser Tyr Thr Ala Gly Glu Asn Asn Ile Leu Asp Leu
115 120 125
Leu Gly Thr Glu Ser Lys Gln Phe Gln Lys Ile Ser Asn Ile Asn Thr
130 135 140
Lys Asp Leu His Arg Ser Ile Thr Ala Ser Asn Thr Gln Ile Gly Tyr
145 150 155 160
Gln Ile Asp Thr Arg Val Pro Gly Pro Cys Lys Gly Val Asp Tyr Gln
165 170 175
Asn Thr Val Thr Tyr Glu Gln Asn Ser Ile Gly Gly Asp Ser Gln Tyr
180 185 190
Leu Ile Phe Tyr Lys Thr Asp Tyr Thr Asp Ala Phe Ile Ile Ala Asn
195 200 205
Arg Ala Asn Gly Arg Val Leu Glu Val Ile Pro Ser Ser Val Asn Gly
210 215 220
Phe Val Thr Ile Ser Asn Met Phe Thr Tyr Asn Gln Asn Gln Leu Phe
225 230 235 240
Ile Arg Thr Lys Ile Ser Asn Asn Asp Asn Ser Asp Asp Val Pro Phe
245 250 255
Ser Leu Thr Thr Glu Asn Asn Gln Thr Leu Asn Ile Cys His His Glu
260 265 270
Phe Gln Tyr Asn Thr Lys Ile Thr Ala Leu Asp Asn Ala Tyr Arg Leu
275 280 285
Asp Asp Lys Val Leu Phe Lys Pro Thr Arg Asp Lys Ile Asn Ile Ser
290 295 300
Phe Pro Asn Met Val Val Asn Ala Lys Glu Lys Leu Pro Glu Pro Glu
305 310 315 320
Glu Leu Thr Asn Met Asp Lys Asn Thr Leu Phe Ile Pro Lys Val Ile
325 330 335
Ile Ser Lys Thr Leu Ile Pro Gly Ile Ile Val Asn Asp Val Thr Leu
340 345 350
Leu Lys Glu Gln Gln Ile Ala Lys Ser Pro Tyr Tyr Val Leu Glu Tyr
355 360 365
Val Gln Ser Trp Glu Glu Val Tyr Asn Glu Ile Val Pro Ala Tyr Arg
370 375 380
Pro Ser Tyr Thr Trp Thr Ser Thr Asp Gly Ile Arg His Val Asn Leu
385 390 395 400
Leu Asp Ile Lys Asn Thr Ile Asn Ile Ser Ile Gly Gly Thr Ser Gln
405 410 415
Gly Trp Gly Leu Arg Phe Ser Asp Lys Ser Asp Leu Phe Lys Asn Ile
420 425 430
Ile Thr Ser Ala Phe Ile Ile Lys Ser Thr Gln Ala Pro Asp Met Gly
435 440 445
Phe Ser Glu Asn Asp Ile Asp Gln Tyr Tyr Gly Lys Asn Ile Asp Ser
450 455 460
Arg Val Lys Ile Tyr Ile Lys Thr His Asn Leu Ile Leu Arg Arg Leu
465 470 475 480
Asp Gln Leu Asn Asn Ser Ile Ala Thr Trp Thr Ile Phe Glu Asn Thr
485 490 495
Lys Pro Val Ile Arg Thr Phe Pro Ile Ser
500 505
<210> 30
<211> 779
<212> PRT
<213> 苏云金芽孢杆菌
<400> 30
Met Lys Asp Lys Lys Tyr Trp Lys Tyr Glu Gly Gly Thr Lys Met Asn
1 5 10 15
Pro Tyr Gln Asn Lys Asn Glu Tyr Glu Ile Val Asn Asn Pro Gln Asn
20 25 30
Tyr Asn Thr Val Ser Asn Arg Tyr Pro Tyr Thr Asn Asp Pro Asn Val
35 40 45
Ala Ile Gln Asn Thr Asn Tyr Lys Asp Trp Met Asn Gly Tyr Glu Glu
50 55 60
Ile Asn Pro Ser Ser Ile Ser Leu Ile Leu Ala Ser Ile Gly Ile Leu
65 70 75 80
Asn Gln Ala Ile Ala Leu Thr Gly Val Leu Gly Lys Thr Pro Glu Ile
85 90 95
Ile Asn Ile Val Gln Glu Met Val Gly Leu Ile Ser Gly Ser Thr Gly
100 105 110
Asn Asp Leu Leu Val His Thr Glu Gln Leu Ile Gln Gln Thr Leu Ala
115 120 125
Gln Gln Tyr Arg Asn Ala Ala Thr Gly Ala Val Asn Ala Ile Ser Lys
130 135 140
Ser Tyr Asn Asp Tyr Leu Met Phe Phe Arg Gln Trp Glu Arg Asn Arg
145 150 155 160
Thr Ser Gln Asn Gly Leu Gln Val Glu Ser Ala Phe Asn Thr Val Asn
165 170 175
Thr Leu Cys Leu Arg Thr Leu Thr Pro Gln Glu Ala Leu Ser Arg Arg
180 185 190
Gly Phe Glu Thr Leu Leu Leu Pro Asn Tyr Ala Leu Ala Ala Asn Phe
195 200 205
His Leu Leu Leu Leu Arg Asp Ala Val Leu Tyr Arg Thr Gln Trp Leu
210 215 220
Pro Asn Phe Ile Ser Thr Thr Asn Ala Asn Ile Glu Ile Leu Glu Arg
225 230 235 240
Ser Ile Asn Gln Tyr Arg Asn His Cys Asn His Trp Tyr Asn Asp Gly
245 250 255
Leu Asn Arg Phe Ala Arg Thr Ser Phe Asp Asp Trp Val Arg Phe Asn
260 265 270
Ala Tyr Arg Arg Asp Met Thr Leu Ser Val Leu Asp Phe Val Thr Val
275 280 285
Phe Pro Thr Tyr Asn Pro Ile Asn Phe Pro Thr Pro Thr Asn Val Glu
290 295 300
Leu Thr Arg Ile Val Tyr Thr Asp Pro Ile Ser Pro Pro Arg Gly Tyr
305 310 315 320
Ala Arg Thr Gly Ser Pro Ser Phe Arg Gln Met Glu Asp Leu Ile Ile
325 330 335
Ser Gly Ser Pro Ser Phe Leu Asn Gln Leu Ser Ile Phe Thr Thr Tyr
340 345 350
Tyr His Asp Pro Arg Asn Val Asn Arg Asp Phe Trp Ala Gly Asn Arg
355 360 365
Asn Tyr Leu Ser Asn Gly Thr Ser Arg Gln Ser Gly Ala Thr Thr Pro
370 375 380
Trp Arg Thr Asn Ile Pro Met Gln Asn Ile Asp Ile Phe Arg Val Asn
385 390 395 400
Leu Thr Thr His Asp Ile Asp Asp Ile Ser Arg Ser Tyr Gly Gly Val
405 410 415
His Arg Ser Asp Phe Ile Gly Val Asn Thr Ile Asn Asn Gln Arg Thr
420 425 430
Thr Leu Phe Tyr His Gln Asn Val Asp Thr Ser Arg Phe Leu Ile Arg
435 440 445
Asn Glu Thr Val Phe Leu Pro Gly Asp Ser Gly Leu Ala Pro Asn Glu
450 455 460
Arg Asn Tyr Thr His Arg Leu Phe Gln Val Met Thr Thr Tyr Arg Thr
465 470 475 480
Asn Pro Asn Ala Arg Arg Ala Ala Phe Leu His Ala Trp Thr His Arg
485 490 495
Ser Leu Arg Arg Arg Asn Gly Phe Arg Thr Asp Gln Ile Met Gln Ile
500 505 510
Pro Ala Val Lys Ser Ile Ser Asn Gly Gly Asp Arg Ala Val Ile Ser
515 520 525
Tyr Thr Gly Glu Asn Met Met Lys Leu Asp Asn Leu Thr Ala Ser Leu
530 535 540
Ser Tyr Lys Leu Thr Ala Glu Asp Ser Glu Ala Ser Asn Thr Arg Phe
545 550 555 560
Ile Val Arg Ile Arg Tyr Ala Ser Met Asn Asn Asn Arg Leu Asn Leu
565 570 575
Ile Leu Asn Gly Thr Gln Ile Ala Ser Leu Asn Val Glu Gly Thr Met
580 585 590
Gln Asn Gly Gly Ser Leu Thr Asn Leu Gln Ser Glu Asn Phe Lys Tyr
595 600 605
Ala Thr Phe Ser Gly Asn Phe Lys Met Gly Ser Gln Ser Ile Val Gly
610 615 620
Ile Phe Lys Glu Ile Ser Asn Ala Asp Phe Ile Leu Asp Lys Ile Glu
625 630 635 640
Leu Ile Pro Ile His Phe Met Pro Leu Leu Glu Gln Lys Gln Ser Tyr
645 650 655
Asn Asn Tyr Asp Gln Asn Met Asp Thr Thr Tyr Gln Pro Asn Tyr Asp
660 665 670
Thr Tyr Asn Gln Asn Ala Asn Gly Met Tyr Asp Asp Thr Tyr Tyr Pro
675 680 685
Asn Asn Asn Asp Ser Tyr Asn Gln Asn Asn Thr Asp Met Tyr Asp Ser
690 695 700
Gly Tyr Asn Asn Asn Gln Asn Thr Asn Tyr Asn Tyr Asp Gln Glu Tyr
705 710 715 720
Asn Thr Tyr Asn Gln Asn Met Glu Asn Thr Tyr Asp Gln Ser Tyr Glu
725 730 735
Asn Tyr Asn Pro Glu Thr Asn Asn Tyr Asn Gln Tyr Pro Asn Asp Met
740 745 750
Tyr Asn Gln Glu Tyr Thr Asn Asp Tyr Asn Gln Asn Ser Gly Cys Arg
755 760 765
Cys Asn Gln Gly Tyr Asn Asn Asn Tyr Pro Lys
770 775
<210> 31
<211> 322
<212> PRT
<213> 苏云金芽孢杆菌
<400> 31
Met Asn Phe Leu Tyr Asn Phe Val Thr Leu Asp Met Leu Ile Leu Asn
1 5 10 15
Arg Leu Glu Gly Ser Asp Phe Lys Met Lys Lys Lys Ala Ile Val Cys
20 25 30
Gly Leu Leu Ala Ser Thr Leu Leu Gly Gly Gly Thr Phe Val Asp Ala
35 40 45
Val Ser Ala Ala Glu Ile Gln Lys Thr Asn His Leu Asn Lys Tyr Asp
50 55 60
Ser Ala Gln Glu Lys Ala Leu Gln Asp Ile Asn Gln Glu Ala Leu Gln
65 70 75 80
Asp Ile Asp Gln Lys Val Asn Lys Met Ile Asp Ser Ile Pro Pro Ile
85 90 95
Phe Gly Ser Lys Tyr Thr Arg Thr Asp Arg Tyr Gly Glu Ser Leu Thr
100 105 110
Tyr Ser Gly Ile Asn Leu Lys Glu Asn Asn Ser Thr Asn Val Glu Pro
115 120 125
Met Tyr Phe Gly Ser Asn Thr Phe Tyr Asn Asp Thr Glu Leu Glu Gln
130 135 140
Ser Tyr Asn Thr Thr Ser Phe Ser Glu Ala Val Thr Lys Ser Thr Thr
145 150 155 160
Thr Gln Thr Gln Asn Gly Phe Lys Ser Gly Val Thr Thr Gly Gly Lys
165 170 175
Val Gly Ile Pro Phe Val Ala Glu Gly Glu Val Lys Ile Asn Leu Glu
180 185 190
Tyr Asn Phe Thr His Thr Asn Ser Asn Thr Thr Ser Lys Thr Thr Thr
195 200 205
Leu Thr Ala Pro Pro Gln Pro Val Lys Val Pro Ala Gly Lys Val Tyr
210 215 220
Lys Ala Asp Val Tyr Phe Glu Lys Lys Ser Thr Ser Gly Thr Val Glu
225 230 235 240
Leu Tyr Gly Asp Leu Leu Thr Gly Val Val Ala Glu Gly Arg Thr Ser
245 250 255
Phe Val Gly Asn Val Leu His Lys Ala Thr Asp Thr Gln Gly Leu Ile
260 265 270
Gln Ser Pro Glu Asp Ser Asn Lys Val Arg Ala Val Gly Lys Gly Thr
275 280 285
Phe Thr Thr Glu His Gly Ser Asn Phe Ile Val Lys Thr Tyr Asp Val
290 295 300
Thr Ser Gly Gln Lys Ser Ala Lys Leu Val Asp Thr Arg Val Ile Pro
305 310 315 320
Ile Lys
<210> 32
<211> 323
<212> PRT
<213> 苏云金芽孢杆菌
<400> 32
Met Asn Leu Arg Arg Ile Ser Met Arg Val Tyr Lys Lys Leu Ala Thr
1 5 10 15
Leu Ala Pro Ile Ala Ala Leu Ser Thr Ser Ile Leu Cys Ser Pro Ala
20 25 30
Met Thr Phe Ala Ala Glu Lys Glu Ser Thr Val Lys Gln Thr Thr Gln
35 40 45
Gln Ser Ala Val Gln Gln Gly Arg Ile Ile Gln Gly Tyr Leu Ile Lys
50 55 60
Asn Gly Val Lys Ile Pro Val Tyr Thr Gly Gly Leu Val Thr Asn Lys
65 70 75 80
Ala Glu Gln Gly Ala Ala Ala Phe Pro Gln Leu Ser Ser Asn Pro Asn
85 90 95
Asp Pro Ile Pro Gln Lys Gly Ser Ile Ser Ser Glu Asp Gly Asn Ile
100 105 110
Gly Asp Ile Leu Tyr Phe Ser Lys Thr Pro Met Gly Asp Asn Val Tyr
115 120 125
Ile Lys Lys Leu Glu Asn Asn Asn Ile Glu Ile Gly Lys Tyr Asn Arg
130 135 140
Gly Thr Leu Glu Leu Ser Lys Phe Val Thr Val Asn Gly Asp Pro Gln
145 150 155 160
Gly Pro Ile Met Leu Phe Asp Ala Thr Val Lys Arg Glu Thr Ala Phe
165 170 175
Glu Lys Ile Gly Gly Ala Val Gln Pro Lys Ala Thr Gln Tyr Thr Phe
180 185 190
Ser Gln Ala Val Thr Ser Gly Leu Ser Thr Ser Asp Ala Ile Gly Gly
195 200 205
Ser Leu Thr Leu Gly Tyr Lys Ile Ser Leu Lys Glu Gly Gly Gly Val
210 215 220
Val Pro Ala Glu Ala Thr Gln Glu Phe Ser Thr Gln Leu Ser Ala Thr
225 230 235 240
Tyr Asn His Thr Ile Thr Val Thr Asn Gln Thr Thr Asn Thr Gln Thr
245 250 255
Gln Thr Phe Lys Pro Ile Asp Ser Tyr Gly Gln Ser Thr Tyr Ala Ala
260 265 270
Ala Val Tyr Gln Leu Lys Ser His Tyr Thr Val Ile Pro Gly Ala Gly
275 280 285
Leu Gln Lys Gly Leu Asn Ser Gly Tyr Val Leu Asp Gln Thr Ala Phe
290 295 300
Ser Tyr Ser Asp Ser Asp Leu Tyr Leu Ala Val Thr Pro Gly Ala Gly
305 310 315 320
Ser Asn Val
<210> 33
<211> 252
<212> PRT
<213> 苏云金芽孢杆菌
<400> 33
Met Ile Thr Asn Gln Ala Ala Gln Ala Ser Asp Ala Pro Tyr Pro Glu
1 5 10 15
Leu Pro Ser Asn Pro Asn Asp Ala Ile Pro Asn Ala Gly Ala Thr His
20 25 30
Ala Glu Asn Gly Ser Val Gly Ser Val Leu Tyr Phe Lys Gln Ile Asp
35 40 45
Leu Asn Asn Leu Gly Ala Gly Ile Gly Asn Ser Gln Lys Asp Tyr Val
50 55 60
Tyr Val Glu Lys Lys Gly Asp Ser Gly Tyr Glu Leu Gly Asn Tyr Asn
65 70 75 80
Pro Leu Thr Leu Gln Arg Thr Lys Ile Lys Asp Tyr Asp Lys Ser Ser
85 90 95
Glu Leu Ala Glu Lys Met Asp Gly Tyr Phe Lys Ser Thr Ile Thr Arg
100 105 110
Asp Thr Phe Phe Ser Lys Ile Gly Ser Gly Val Val Pro Lys Asn Ala
115 120 125
Ala Tyr Thr Phe Ser Gln Ala Val Thr Ser Gly Leu Thr Thr Ser Asp
130 135 140
Ala Ile Gly Gly Ala Leu Thr Leu Gly Tyr Lys Val Ser Val Thr Glu
145 150 155 160
Gly Gly Gly Ile Phe Pro Ala Ala Ala Ser Glu Glu Phe Ser Ala Gln
165 170 175
Leu Thr Ala Thr Tyr Asn His Thr Ile Thr Val Ser Ser Gln Val Thr
180 185 190
Asn Thr Gln Thr Leu Gly Ile Thr Lys Ala Ala Asp Gly Tyr Gln Tyr
195 200 205
Asp Lys Tyr Val Gly Ala Val Tyr Gln Leu His Ser Lys Tyr Thr Phe
210 215 220
Lys Pro Ser Asp Glu Leu Gln Phe Ala Met Asn Ser Pro Phe Gly Tyr
225 230 235 240
Lys Val Ile Leu Asn Gln Arg Ala Gln Ser Phe Gln
245 250
<210> 34
<211> 1441
<212> PRT
<213> 苏云金芽孢杆菌
<400> 34
Met Thr Thr Ile Asn Glu Leu Tyr Pro Ala Val Pro Tyr Asn Val Leu
1 5 10 15
Ala Tyr Ala Pro Pro Leu Asn Leu Ala Asp Ser Thr Pro Trp Gly Gln
20 25 30
Ile Val Val Ala Asp Ala Ile Lys Glu Ala Trp Asp Asn Phe Gln Lys
35 40 45
Tyr Gly Val Leu Asp Leu Thr Ala Ile Asn Gln Gly Phe Asp Asp Ala
50 55 60
Asn Thr Gly Ser Phe Ser Tyr Gln Ala Leu Ile Gln Thr Val Leu Gly
65 70 75 80
Ile Ile Gly Thr Ile Gly Met Thr Val Pro Val Ala Ala Pro Phe Ala
85 90 95
Ala Thr Ala Pro Ile Ile Ser Leu Phe Val Gly Phe Phe Trp Pro Lys
100 105 110
Lys Asp Lys Gly Pro Gln Leu Ile Asp Ile Ile Asp Lys Glu Ile Lys
115 120 125
Lys Leu Leu Asp Lys Glu Leu Gly Glu Gln Lys Arg Asn Asp Leu Val
130 135 140
Ser Ala Leu Asn Glu Met Gln Glu Gly Ala Asn Glu Leu Ser Asp Ile
145 150 155 160
Met Thr Asn Ala Leu Phe Glu Gly Thr Ile Gln Gly Asn Val Val Thr
165 170 175
Asn Asp Asn Pro Gln Gly Lys Arg Arg Thr Pro Lys Ala Pro Thr Val
180 185 190
Ser Asp Tyr Glu Asn Val Tyr Ser Ala Tyr Phe Val Glu His Val Asp
195 200 205
Phe Arg Asn Lys Ile Ser Thr Phe Leu Thr Gly Ser Tyr Asp Leu Ile
210 215 220
Ala Leu Pro Leu Tyr Ala Leu Ala Lys Thr Met Glu Leu Ser Leu Tyr
225 230 235 240
Gln Ser Phe Ile Asn Phe Ala Asn Lys Trp Met Asp Phe Val Tyr Thr
245 250 255
Lys Ala Ile Asn Glu Ser Ala Thr Asp Asp Met Lys Arg Asp Tyr Gln
260 265 270
Ala Arg Tyr Asn Thr Gln Lys Ser Asn Leu Ala Val Gln Lys Thr Gln
275 280 285
Leu Ile Asn Lys Ile Lys Asp Gly Thr Asp Ala Val Met Lys Val Phe
290 295 300
Lys Asp Thr Asn Asn Leu Pro Ser Ile Gly Thr Asn Lys Leu Ala Val
305 310 315 320
Asn Ala Arg Asn Lys Tyr Ile Arg Ala Leu Gln Ile Asn Cys Leu Asp
325 330 335
Leu Val Ala Leu Trp Pro Gly Leu Tyr Pro Asp Glu Tyr Leu Leu Pro
340 345 350
Leu Gln Leu Asp Lys Thr Arg Val Val Phe Ser Asp Thr Met Gly Pro
355 360 365
Asp Glu Thr His Asp Gly Gln Met Lys Val Leu Asn Ile Leu Asp Ser
370 375 380
Thr Thr Ser Tyr Asn His Gln Asp Ile Gly Ile Ser Thr Thr Gln Asp
385 390 395 400
Val Asn Ser Leu Leu Phe Tyr Pro Arg Lys Glu Leu Leu Glu Leu Asp
405 410 415
Phe Ala Lys Tyr Ile Ser Ser Ser Ser Arg Phe Trp Val Tyr Gly Phe
420 425 430
Gly Leu Lys Tyr Ser Asp Asp Asn Phe Tyr Arg Tyr Gly Asp Asn Asp
435 440 445
Pro Ser Ser Asp Phe Lys Pro Ala Tyr Lys Trp Phe Thr Lys Asn Ser
450 455 460
Gln Phe Glu Asn Leu Pro Thr Tyr Gly Asn Pro Thr Pro Ile Thr Asn
465 470 475 480
Leu Asn Ala Lys Thr Gln Val Thr Ser Tyr Leu Asp Ala Leu Ile Tyr
485 490 495
Tyr Ile Asp Gly Gly Thr Asn Leu Tyr Asn Asn Ala Ile Leu His Asp
500 505 510
Thr Gly Gly Tyr Ile Pro Gly Tyr Pro Gly Val Glu Gly Tyr Gly Met
515 520 525
Ser Asn Asn Glu Pro Leu Ala Gly Gln Lys Leu Asn Ala Leu Tyr Pro
530 535 540
Ile Lys Val Glu Asn Val Ser Gly Ser Gln Gly Lys Leu Gly Thr Ile
545 550 555 560
Ala Ala Tyr Val Pro Leu Asn Leu Gln Pro Glu Asn Ile Ile Gly Asp
565 570 575
Ala Asp Pro Asn Thr Gly Phe Pro Leu Asn Val Ile Lys Gly Phe Pro
580 585 590
Phe Glu Lys Tyr Gly Pro Asp Tyr Glu Gly Arg Gly Ile Ser Val Val
595 600 605
Lys Glu Trp Ile Asn Gly Ala Asn Ala Val Lys Leu Ser Pro Gly Gln
610 615 620
Ser Val Gly Val Gln Ile Lys Asn Ile Thr Lys Gln Asn Tyr Gln Ile
625 630 635 640
Arg Thr Arg Tyr Ala Ser Asn Asn Ser Asn Gln Val Tyr Phe Asn Val
645 650 655
Asp Pro Gly Gly Ser Pro Leu Phe Ala Gln Ser Val Thr Phe Glu Ser
660 665 670
Thr Thr Asn Val Thr Ser Gly Gln Gln Gly Glu Asn Gly Arg Tyr Thr
675 680 685
Leu Lys Thr Ile Phe Ser Gly Asn Asp Leu Leu Thr Val Glu Ile Pro
690 695 700
Val Gly Asn Phe Tyr Val His Val Thr Asn Lys Gly Ser Ser Asp Ile
705 710 715 720
Phe Leu Asp Arg Leu Glu Phe Ser Thr Val Pro Ser Tyr Val Ile Tyr
725 730 735
Ser Gly Asp Tyr Asp Ala Thr Gly Thr Asp Asp Val Leu Leu Ser Asp
740 745 750
Pro His Glu Tyr Phe Tyr Asp Val Ile Val Asn Gly Thr Ala Ser His
755 760 765
Ser Ser Ala Ala Thr Ser Met Asn Leu Leu Asn Lys Gly Thr Val Val
770 775 780
Arg Ser Ile Asp Ile Pro Gly His Ser Thr Ser Tyr Ser Val Gln Tyr
785 790 795 800
Ser Val Pro Glu Gly Phe Asp Glu Val Arg Ile Leu Ser Ser Leu Pro
805 810 815
Asp Ile Ser Gly Thr Ile Arg Val Glu Ser Ser Lys Pro Pro Val Phe
820 825 830
Lys Asn Asp Gly Asn Ser Gly Asp Gly Gly Asn Thr Glu Tyr Asn Phe
835 840 845
Asn Phe Asp Leu Ser Gly Leu Gln Asp Thr Gly Leu Tyr Ser Gly Lys
850 855 860
Leu Lys Ser Gly Ile Arg Val Gln Gly Asn Tyr Thr Tyr Thr Gly Ala
865 870 875 880
Pro Ser Leu Asn Leu Val Val Tyr Arg Asn Asn Ser Val Val Ser Thr
885 890 895
Phe Pro Val Gly Ser Pro Phe Asp Ile Thr Ile Thr Thr Glu Thr Asp
900 905 910
Lys Val Ile Leu Ser Leu Gln Pro Gln His Gly Leu Ala Thr Val Thr
915 920 925
Gly Thr Gly Thr Ile Thr Ile Pro Asn Asp Lys Leu Ala Ile Val Tyr
930 935 940
Asp Lys Leu Phe Lys Leu Pro His Asp Leu Glu Asn Ile Arg Ile Gln
945 950 955 960
Val Asn Ala Leu Phe Ile Ser Ser Thr Gln Asn Glu Leu Ala Lys Glu
965 970 975
Val Asn Asp His Asp Ile Glu Glu Val Ala Leu Lys Val Asp Ala Leu
980 985 990
Ser Asp Glu Val Phe Gly Lys Glu Lys Lys Glu Leu Arg Lys Leu Val
995 1000 1005
Asn Gln Ala Lys Arg Leu Ser Lys Ala Arg Asn Leu Leu Val Gly Gly
1010 1015 1020
Asn Phe Asp Asn Trp Glu Ala Trp Tyr Lys Gly Lys Glu Val Ala Arg
1025 1030 1035 1040
Val Ser Asp His Glu Leu Leu Lys Ser Asp His Val Leu Leu Pro Pro
1045 1050 1055
Pro Thr Met Tyr Pro Ser Tyr Ile Tyr Gln Lys Val Glu Glu Thr Lys
1060 1065 1070
Leu Lys Pro Asn Thr Arg Tyr Met Ile Ser Gly Phe Ile Ala His Ala
1075 1080 1085
Glu Asp Leu Glu Ile Val Val Ser Arg Tyr Gly Gln Glu Val Arg Lys
1090 1095 1100
Ile Val Gln Val Pro Tyr Gly Glu Ala Phe Pro Leu Thr Ser Asn Gly
1105 1110 1115 1120
Ser Ile Cys Cys Thr Pro Ser Phe Arg Arg Asp Gly Lys Leu Ser Asp
1125 1130 1135
Pro His Phe Phe Ser Tyr Ser Ile Asp Val Gly Glu Leu Asp Met Thr
1140 1145 1150
Ala Gly Pro Gly Ile Glu Leu Gly Leu Arg Ile Val Asp Arg Leu Gly
1155 1160 1165
Met Ala Arg Val Ser Asn Leu Glu Ile Arg Glu Asp Arg Ser Leu Thr
1170 1175 1180
Ala Asn Glu Ile Arg Lys Val Gln Arg Met Ala Arg Asn Trp Arg Thr
1185 1190 1195 1200
Glu Tyr Glu Lys Glu Arg Ala Glu Val Thr Ala Leu Ile Glu Pro Val
1205 1210 1215
Leu Asn Gln Ile Asn Ala Leu Tyr Glu Asn Gly Asp Trp Asn Gly Ser
1220 1225 1230
Ile Arg Ser Asp Ile Ser Tyr Tyr Asp Ile Glu Ser Ile Val Leu Pro
1235 1240 1245
Thr Leu Pro Arg Leu Arg His Trp Phe Val Pro Asp Met Leu Thr Glu
1250 1255 1260
His Gly Asn Ile Met Asn Arg Phe Glu Glu Ala Leu Asn Arg Ala Tyr
1265 1270 1275 1280
Thr Gln Leu Glu Gly Asn Thr Leu Leu His Asn Gly His Phe Thr Thr
1285 1290 1295
Asp Ala Val Asn Trp Met Ile Gln Gly Asp Ala His Gln Val Ile Leu
1300 1305 1310
Glu Asp Gly Arg Arg Val Leu Arg Leu Pro Asp Trp Ser Ser Ser Val
1315 1320 1325
Ser Gln Thr Ile Glu Ile Glu Lys Phe Asp Pro Asp Lys Glu Tyr Asn
1330 1335 1340
Leu Val Phe His Ala Gln Gly Glu Gly Thr Val Thr Leu Glu His Gly
1345 1350 1355 1360
Glu Lys Thr Lys Tyr Ile Glu Thr His Thr His His Phe Ala Asn Phe
1365 1370 1375
Thr Thr Ser Gln Ser Gln Gly Ile Thr Phe Glu Ser Asn Lys Val Thr
1380 1385 1390
Val Glu Ile Ser Ser Glu Asp Gly Glu Leu Leu Val Asp His Ile Ala
1395 1400 1405
Leu Val Glu Val Pro Met Phe Asn Lys Asn Gln Met Val Asn Glu Asn
1410 1415 1420
Arg Asp Val Asn Ile Asn Ser Asn Thr Asn Met Asn Asn Ser Asn Asn
1425 1430 1435 1440
Gln
<210> 35
<211> 731
<212> PRT
<213> 苏云金芽孢杆菌
<400> 35
Met Thr Thr Ile Asn Glu Leu Tyr Pro Ala Val Pro Tyr Asn Val Leu
1 5 10 15
Ala Tyr Ala Pro Pro Leu Asn Leu Ala Asp Ser Thr Pro Trp Gly Gln
20 25 30
Ile Val Val Ala Asp Ala Ile Lys Glu Ala Trp Asp Asn Phe Gln Lys
35 40 45
Tyr Gly Val Leu Asp Leu Thr Ala Ile Asn Gln Gly Phe Asp Asp Ala
50 55 60
Asn Thr Gly Ser Phe Ser Tyr Gln Ala Leu Ile Gln Thr Val Leu Gly
65 70 75 80
Ile Ile Gly Thr Ile Gly Met Thr Val Pro Val Ala Ala Pro Phe Ala
85 90 95
Ala Thr Ala Pro Ile Ile Ser Leu Phe Val Gly Phe Phe Trp Pro Lys
100 105 110
Lys Asp Lys Gly Pro Gln Leu Ile Asp Ile Ile Asp Lys Glu Ile Lys
115 120 125
Lys Leu Leu Asp Lys Glu Leu Gly Glu Gln Lys Arg Asn Asp Leu Val
130 135 140
Ser Ala Leu Asn Glu Met Gln Glu Gly Ala Asn Glu Leu Ser Asp Ile
145 150 155 160
Met Thr Asn Ala Leu Phe Glu Gly Thr Ile Gln Gly Asn Val Val Thr
165 170 175
Asn Asp Asn Pro Gln Gly Lys Arg Arg Thr Pro Lys Ala Pro Thr Val
180 185 190
Ser Asp Tyr Glu Asn Val Tyr Ser Ala Tyr Phe Val Glu His Val Asp
195 200 205
Phe Arg Asn Lys Ile Ser Thr Phe Leu Thr Gly Ser Tyr Asp Leu Ile
210 215 220
Ala Leu Pro Leu Tyr Ala Leu Ala Lys Thr Met Glu Leu Ser Leu Tyr
225 230 235 240
Gln Ser Phe Ile Asn Phe Ala Asn Lys Trp Met Asp Phe Val Tyr Thr
245 250 255
Lys Ala Ile Asn Glu Ser Ala Thr Asp Asp Met Lys Arg Asp Tyr Gln
260 265 270
Ala Arg Tyr Asn Thr Gln Lys Ser Asn Leu Ala Val Gln Lys Thr Gln
275 280 285
Leu Ile Asn Lys Ile Lys Asp Gly Thr Asp Ala Val Met Lys Val Phe
290 295 300
Lys Asp Thr Asn Asn Leu Pro Ser Ile Gly Thr Asn Lys Leu Ala Val
305 310 315 320
Asn Ala Arg Asn Lys Tyr Ile Arg Ala Leu Gln Ile Asn Cys Leu Asp
325 330 335
Leu Val Ala Leu Trp Pro Gly Leu Tyr Pro Asp Glu Tyr Leu Leu Pro
340 345 350
Leu Gln Leu Asp Lys Thr Arg Val Val Phe Ser Asp Thr Met Gly Pro
355 360 365
Asp Glu Thr His Asp Gly Gln Met Lys Val Leu Asn Ile Leu Asp Ser
370 375 380
Thr Thr Ser Tyr Asn His Gln Asp Ile Gly Ile Ser Thr Thr Gln Asp
385 390 395 400
Val Asn Ser Leu Leu Phe Tyr Pro Arg Lys Glu Leu Leu Glu Leu Asp
405 410 415
Phe Ala Lys Tyr Ile Ser Ser Ser Ser Arg Phe Trp Val Tyr Gly Phe
420 425 430
Gly Leu Lys Tyr Ser Asp Asp Asn Phe Tyr Arg Tyr Gly Asp Asn Asp
435 440 445
Pro Ser Ser Asp Phe Lys Pro Ala Tyr Lys Trp Phe Thr Lys Asn Ser
450 455 460
Gln Phe Glu Asn Leu Pro Thr Tyr Gly Asn Pro Thr Pro Ile Thr Asn
465 470 475 480
Leu Asn Ala Lys Thr Gln Val Thr Ser Tyr Leu Asp Ala Leu Ile Tyr
485 490 495
Tyr Ile Asp Gly Gly Thr Asn Leu Tyr Asn Asn Ala Ile Leu His Asp
500 505 510
Thr Gly Gly Tyr Ile Pro Gly Tyr Pro Gly Val Glu Gly Tyr Gly Met
515 520 525
Ser Asn Asn Glu Pro Leu Ala Gly Gln Lys Leu Asn Ala Leu Tyr Pro
530 535 540
Ile Lys Val Glu Asn Val Ser Gly Ser Gln Gly Lys Leu Gly Thr Ile
545 550 555 560
Ala Ala Tyr Val Pro Leu Asn Leu Gln Pro Glu Asn Ile Ile Gly Asp
565 570 575
Ala Asp Pro Asn Thr Gly Phe Pro Leu Asn Val Ile Lys Gly Phe Pro
580 585 590
Phe Glu Lys Tyr Gly Pro Asp Tyr Glu Gly Arg Gly Ile Ser Val Val
595 600 605
Lys Glu Trp Ile Asn Gly Ala Asn Ala Val Lys Leu Ser Pro Gly Gln
610 615 620
Ser Val Gly Val Gln Ile Lys Asn Ile Thr Lys Gln Asn Tyr Gln Ile
625 630 635 640
Arg Thr Arg Tyr Ala Ser Asn Asn Ser Asn Gln Val Tyr Phe Asn Val
645 650 655
Asp Pro Gly Gly Ser Pro Leu Phe Ala Gln Ser Val Thr Phe Glu Ser
660 665 670
Thr Thr Asn Val Thr Ser Gly Gln Gln Gly Glu Asn Gly Arg Tyr Thr
675 680 685
Leu Lys Thr Ile Phe Ser Gly Asn Asp Leu Leu Thr Val Glu Ile Pro
690 695 700
Val Gly Asn Phe Tyr Val His Val Thr Asn Lys Gly Ser Ser Asp Ile
705 710 715 720
Phe Leu Asp Arg Leu Glu Phe Ser Thr Val Pro
725 730
<210> 36
<211> 364
<212> PRT
<213> 苏云金芽孢杆菌
<400> 36
Met Leu Ile Lys Glu Met Gln Tyr Met His Ser Ile Lys Lys Tyr Lys
1 5 10 15
Lys Val Leu Leu Ile Ala Pro Leu Ala Cys Met Leu Thr Gly Ala Ile
20 25 30
Leu Pro Thr Ala Thr Thr Val His Ala Gln Glu Val Glu Asn Lys Lys
35 40 45
Ala Val Ser Met Met Lys Pro Gly Gly Glu Phe Gly Ala Thr Lys Tyr
50 55 60
Ser Lys Glu Asn Leu Val Lys Glu Ile Asn Leu Arg Leu Leu Thr Ala
65 70 75 80
Leu Asp Arg Ser Thr Ser Leu Arg Glu Lys Phe His Ile Lys Gly Asn
85 90 95
Glu Val Leu Asp Val Ser Gln Leu Asp Asp Thr Ser Lys Gln Leu Met
100 105 110
Glu Lys Leu Gln Leu Thr Ala Glu Gly Ser Ile Asp Val Lys Pro His
115 120 125
Val Asp Ser Tyr Lys Asp Leu Gly Gln Thr Asn Ile Val Thr Tyr Asn
130 135 140
Asn Asp Asn Gly Val Val Gly Gln Thr Tyr Asn Thr Pro Glu Thr Thr
145 150 155 160
Val Lys Glu Ser Glu Thr His Thr Tyr Ser Asn Thr Glu Gly Val Lys
165 170 175
Leu Gly Leu Glu Val Gly Thr Lys Ile Thr Val Gly Ile Pro Phe Ile
180 185 190
Gly Lys Asp Glu Thr Glu Ile Lys Ala Thr Ser Glu Phe Ser Tyr Glu
195 200 205
His Asn Asp Ser Gln Thr Lys Thr Lys Glu Thr Asp Val Thr Phe Lys
210 215 220
Ser Gln Pro Val Val Ala Ala Pro Gly Gly Thr Thr Thr Tyr Tyr Gly
225 230 235 240
Asp Ile Lys Thr Ala Thr Phe Ser Gly Ser Phe Gln Ser Asp Ala Tyr
245 250 255
Val Ala Gly Gly Phe Glu Leu Lys Val Pro Ile Ala His Asp Met Ala
260 265 270
Ser Pro Lys Ile Asp Arg Tyr Glu Thr Ala Thr Leu Thr Ala Ala Asp
275 280 285
Ile Tyr Glu Ile Phe Asn Ala Ser Asn Ala Ile Ala Ala Pro Asn Tyr
290 295 300
Leu Lys Leu Asp Asn Ala Gly Lys Lys Val Leu Leu Thr Asp Lys Ala
305 310 315 320
Thr Phe Asp Ile Asn Gly Gln Gly Gly Phe Tyr Thr Thr Leu Gln Val
325 330 335
Lys Phe Val Pro Lys Asp Ser Asn Lys Lys Pro Gln Met Met Ser Tyr
340 345 350
Lys Glu Tyr Val Gln Lys Met Asn Asn Asn Glu Leu
355 360
<210> 37
<211> 394
<212> PRT
<213> 苏云金芽孢杆菌
<400> 37
Met Tyr Ser Ile Lys Arg Tyr Lys Lys Val Ala Ile Val Ala Pro Leu
1 5 10 15
Val Cys Leu Leu Gly Thr Gly Leu Thr Phe Val Asn Lys Pro Ile Pro
20 25 30
Ala Ala Ala Ala Val Thr Thr Asn Tyr Ser Thr Ala Asp Ser Ala Ser
35 40 45
Asn Phe Gln Pro Ile Ser Lys Tyr Thr Leu Ala Gly Asp Leu Tyr Glu
50 55 60
Arg Tyr Met Arg Ala Leu Val Arg His Pro Glu Leu Leu Ser Ser Gly
65 70 75 80
Gly Leu Lys Pro Val Thr Asn Gln Thr Asp Leu Glu Gln Ile Asp Gly
85 90 95
Tyr Tyr Lys Val Met Ala Gln Phe Ile Arg Asp Asn Asn Gln Asn Phe
100 105 110
Pro Ser Pro Phe Asn Arg Pro Ser Met Lys Leu Met Thr Gly Val Asn
115 120 125
Pro Phe Phe Asn Trp Ala Pro Gln Tyr Thr Asn Leu Ser Thr Gln Asn
130 135 140
Val Ile Asn Leu Asp Asn Pro Lys Val Asp Asp Tyr Lys Glu Asp Asn
145 150 155 160
Ile Glu Leu Ala Thr Tyr Thr Asn Asn Thr Thr Ser Glu Gln Thr Phe
165 170 175
Ser Thr Pro Ser Lys Ser Glu Lys Val Thr Asp Ser Phe Thr Tyr Ser
180 185 190
Asn Ser Glu Gly Gly Lys Leu Gly Val Ser Ser Thr Thr Thr Ile Arg
195 200 205
Ala Gly Ile Pro Ile Ala Gln Ala Gln Glu Thr Leu Thr Met Ser Phe
210 215 220
Glu Ala Thr Tyr Asn His Thr Ser Ser Asn Thr Ser Ser Thr Glu Lys
225 230 235 240
Thr Val Thr Tyr Pro Ser Gln Val Leu Lys Cys Leu Pro Gly Tyr Arg
245 250 255
Thr Ser Leu Ile Val Lys Val Ser Gln Ala Asn Phe Ser Gly Thr Met
260 265 270
Asp Phe Asp Val Glu Pro Thr Val Ser Ser Leu Ile Asp Gly Ile Glu
275 280 285
Lys Asn Trp Lys Asp Ile Lys Asp Asp Lys Thr Ile Lys Gly Asp Lys
290 295 300
Ser Gly Asp Tyr Thr Val Pro Asn Arg Gln Glu Phe Leu Tyr Asn Val
305 310 315 320
Tyr Lys Tyr Ser Asp Leu Pro Ile Pro Ser Tyr Val Lys Leu Asp Asp
325 330 335
Lys Lys Lys Thr Val Ser Phe Gly Lys Val Thr Thr Pro Tyr Thr Gly
340 345 350
Val Ala Gly His Leu Ser Glu Ala Asn Ala Thr Gln Val Lys Leu Glu
355 360 365
Ser Leu Asp Lys Ala Gln Lys Pro Ile Ile Met Pro Leu Lys Gln Tyr
370 375 380
Gln Gln Lys Ile Gln Asn His Glu Ser Phe
385 390
<210> 38
<211> 360
<212> PRT
<213> 苏云金芽孢杆菌
<400> 38
Met His Ser Ile Lys Lys Tyr Lys Lys Ile Leu Leu Val Ala Pro Leu
1 5 10 15
Ala Cys Met Leu Thr Gly Ala Ile Leu Pro Thr Ala Thr Thr Val His
20 25 30
Ala Gln Glu Ile Lys Gly Pro Gly Val Met Lys Pro Asp Val Pro Trp
35 40 45
Asn Gln Glu His Tyr Thr Lys Glu Asn Leu Ala Trp Arg Ala Ala Asp
50 55 60
Arg Leu Ser Tyr Ala Ala Asp Arg Ile Pro Ser Leu Arg Glu Lys Phe
65 70 75 80
Lys Leu Lys Pro Asn Glu His Phe Tyr Cys Ser Asn Asp Thr Arg Tyr
85 90 95
Tyr Met Glu Glu Thr Leu Leu Lys Asn Leu Gln Leu Ser Ala Glu Gly
100 105 110
Pro Ile Asn Val Thr Pro His Val Asp Ser Tyr Thr Asp Leu Gly Gln
115 120 125
Thr Asn Leu Leu Thr Tyr Asn Asn Asp Asp Gly Ile Val Glu Gln Lys
130 135 140
Ala Ser Thr Pro Glu Thr Thr Ile Lys Glu Ser Glu Thr Ser Ser Tyr
145 150 155 160
Ser Asn Lys Glu Gly Val Thr Leu Gly Ala Glu Val Glu Ser Lys Val
165 170 175
Thr Phe Asn Ile Pro Phe Ile Val Ala Gly Glu Thr Lys Val Ile Ala
180 185 190
Lys Ser Glu Phe Ser Tyr Glu His Asp Asp Thr Gln Thr Lys Thr His
195 200 205
Glu Lys Glu Val Thr Phe Lys Ser Gln Glu Ile Val Ala Ala Pro Glu
210 215 220
Gly Thr Thr Thr Tyr Tyr Gly Ser Ile Lys Thr Ala Asn Phe Ser Gly
225 230 235 240
Ser Phe Gln Ser Asp Ala Val Val Gly Gly Gly Val Thr Leu Thr Leu
245 250 255
Pro Ile Gly Val Met Asp Lys Asp Gly Gly Gln Lys Lys Thr His Thr
260 265 270
Glu Thr Ala Thr Leu Thr Ala Glu Asp Met Tyr Glu Ile Phe Lys Ala
275 280 285
Pro Met Pro Trp Asp Met Asn Lys Leu Pro Pro Tyr Leu Lys Leu Asp
290 295 300
Asp Ser Gly Lys Arg Val Leu Leu Ala Glu Lys Ala Thr Phe Asp Ile
305 310 315 320
Lys Gly Gln Gly Gly Phe Tyr Thr Glu Ile Gln Ala Lys Phe Val Pro
325 330 335
Lys Asp Lys Asn Lys Lys Thr Gln Ile Met Pro Tyr Ala Glu Tyr Val
340 345 350
Gln Lys Val Lys Gln Asn Ala Leu
355 360
<210> 39
<211> 676
<212> PRT
<213> 苏云金芽孢杆菌
<400> 39
Met Lys Ser Met Asn Ser Tyr Gln Asn Lys Asn Glu Tyr Glu Ile Leu
1 5 10 15
Asp Ala Ser Gln Asn Asn Ser Thr Met Ser Thr Arg Tyr Pro Arg Tyr
20 25 30
Pro Leu Ala Lys Asp Pro Gln Ala Ser Met Gln Thr Thr Asn Tyr Lys
35 40 45
Asp Trp Leu Asn Leu Cys Asp Thr Pro Asn Met Glu Asn Pro Glu Phe
50 55 60
Gln Ser Val Gly Arg Ser Ala Leu Ser Ile Leu Ile Asn Leu Ser Ser
65 70 75 80
Arg Ile Leu Ser Leu Leu Gly Ile Pro Phe Ala Ala Gln Ile Gly Gln
85 90 95
Leu Trp Ser Tyr Thr Leu Asn Leu Leu Trp Pro Val Ala Asn Asn Ala
100 105 110
Thr Gln Trp Glu Ile Phe Met Arg Thr Ile Glu Glu Leu Ile Asn Ala
115 120 125
Arg Ile Glu Thr Ser Val Arg Asn Arg Ala Leu Ala Glu Leu Ala Gly
130 135 140
Leu Gly Asn Ile Leu Glu Asp Tyr Lys Val Val Leu Gln Arg Trp Asn
145 150 155 160
Leu Asn Pro Thr Asn Pro Thr Leu Gln Arg Asp Val Val Arg Gln Phe
165 170 175
Glu Ile Val His Ala Phe Phe Arg Phe Gln Met Pro Val Phe Ala Val
180 185 190
Asp Gly Phe Glu Val Pro Leu Leu Pro Val Tyr Ala Ser Ala Ala Asn
195 200 205
Leu His Leu Leu Leu Leu Arg Asp Val Val Ile Asn Gly Ala Arg Trp
210 215 220
Gly Leu Glu Ser Asp Val Ile Asn Asp Tyr His Asp Leu Gln Leu Arg
225 230 235 240
Leu Thr Ser Thr Tyr Val Asp His Cys Val Thr Trp Tyr Asn Thr Gly
245 250 255
Leu Asn Arg Leu Ile Gly Thr Asn Ala Arg Gln Trp Val Thr Tyr Asn
260 265 270
Gln Phe Arg Arg Glu Met Thr Ile Ser Val Leu Asp Ile Ile Ser Leu
275 280 285
Phe Ser Asn Tyr Asp Val Arg Arg Tyr Pro Thr Lys Thr Gln Ser Glu
290 295 300
Leu Thr Arg Met Ile Tyr Thr Asp Pro Ile Gly Thr Glu Gly Asn Gln
305 310 315 320
Phe Ile Pro Gly Trp Val Asp Asn Ala Pro Ser Phe Ser Val Ile Glu
325 330 335
Asn Ser Val Val Arg Ser Pro Gly Ala Phe Thr Phe Leu Glu Arg Val
340 345 350
Gly Ile Phe Thr Gly Phe Leu His Gly Trp Ser Ser Arg Ser Glu Phe
355 360 365
Trp Ser Ala His Arg Leu Phe Ser Arg Pro Val Leu Gly Trp Ile Trp
370 375 380
Glu Ser Val Ile Phe Gly Asn Pro Gln Asn Asn Ile Gly Tyr Gln Glu
385 390 395 400
Val Asp Phe Thr Asn Phe Asp Val Phe Ser Ile Asn Ser Arg Ala Thr
405 410 415
Ser His Met Phe Pro Asn Gly Ser Ala Arg Leu Phe Gly Val Pro Arg
420 425 430
Val Thr Phe Asp Leu Ser Asn Val Thr Asn Asn Asn Leu Ala Gln Arg
435 440 445
Thr Tyr Asn Arg Pro Phe Thr Phe Gly Gly Gln Asp Ile Val Ser Arg
450 455 460
Leu Pro Gly Glu Thr Thr Glu Ile Pro Asn Ser Ser Asn Phe Ser His
465 470 475 480
Arg Leu Ala His Ile Ser Ser Phe Pro Val Gly Asn Asn Gly Ser Val
485 490 495
Leu Ser Tyr Gly Trp Thr His Arg Asn Val Asn Arg His Asn Arg Leu
500 505 510
Asn Pro Asn Ser Ile Thr Gln Ile Pro Ala Ile Lys Phe Ala Ser Gly
515 520 525
Ser Ala Arg Arg Gly Pro Gly His Thr Gly Gly Asp Leu Ala Ile Ala
530 535 540
Gln Gln His Ser Gly Tyr Gln Leu Phe Met Gln Ser Pro Ser Ala Gln
545 550 555 560
Arg Tyr Arg Leu Arg Leu Arg Tyr Ala Gly Ile Ser Gly Gly Ser Ile
565 570 575
Ser Val Ser His Arg Asp Glu Asn Asn Gln Asn Ile Leu His Ser Ala
580 585 590
Thr Phe Asn Val Arg Ala Thr Ser Gly Gln Leu Arg Tyr Ala Asp Phe
595 600 605
Ile Tyr Thr Asp Leu Glu Glu Asn Thr Thr Leu Phe Glu Thr Arg Asn
610 615 620
Gly Val Asn Leu Tyr Arg Leu Met Ile Phe Val Ser Ser Gly Ser Ile
625 630 635 640
Leu Ile Asp Arg Ile Glu Tyr Ile Pro Glu Asn Thr Thr Thr Ile Glu
645 650 655
Tyr Glu Glu Glu Arg Asn Leu Glu Lys Glu Lys Lys Ala Val Asp Asp
660 665 670
Leu Phe Thr Asn
675
<210> 40
<211> 567
<212> PRT
<213> 苏云金芽孢杆菌
<400> 40
Met Asn Asn Met Tyr Thr Asn Asn Met Lys Thr Thr Leu Lys Leu Glu
1 5 10 15
Thr Thr Asp Tyr Glu Ile Asp Gln Ala Ala Ile Ser Ile Glu Cys Met
20 25 30
Ser Asp Glu Gln Asp Leu Gln Glu Lys Met Met Leu Trp Asp Glu Val
35 40 45
Lys Leu Ala Lys Gln Leu Ser Gln Ser Arg Asn Leu Leu Tyr Asn Gly
50 55 60
Asp Phe Glu Asp Ser Ser Asn Gly Trp Lys Thr Ser Asn Asn Ile Thr
65 70 75 80
Ile Gln Leu Glu Asn Pro Ile Leu Lys Gly Lys Tyr Leu Asn Met Pro
85 90 95
Gly Ala Arg Asp Ile Tyr Gly Thr Ile Phe Pro Thr Tyr Val Tyr Gln
100 105 110
Lys Ile Asp Glu Ser Lys Leu Lys Pro Asn Thr Arg Tyr Arg Val Arg
115 120 125
Gly Phe Val Gly Ser Ser Lys Asp Leu Lys Leu Val Val Thr Arg Tyr
130 135 140
Glu Lys Glu Ile Asp Ala Ser Met Asp Val Pro Asn Asp Leu Ser Tyr
145 150 155 160
Met Gln Pro Ser Pro Ser Cys Gly Asp Tyr Gly Cys Asp Ser Ser Ser
165 170 175
Gln Pro Met Met Asn Gln Gly Tyr Pro Thr Pro Tyr Thr Asp Asp Tyr
180 185 190
Ala Ser Asp Met Tyr Ala Cys Ser Ser Asn Leu Gly Lys Lys His Val
195 200 205
Lys Cys His Asp Arg His Pro Phe Asp Phe His Ile Asp Thr Gly Glu
210 215 220
Leu Asp Thr Asn Thr Asn Leu Gly Ile Cys Ile Leu Phe Lys Ile Ser
225 230 235 240
Asn Pro Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Val Ile Glu Glu
245 250 255
Gly Pro Leu Thr Ser Glu Ala Leu Ala His Val Asn Gln Lys Glu Lys
260 265 270
Lys Trp Asn Gln Gln Met Glu Lys Lys Arg Ser Glu Thr Gln Gln Ala
275 280 285
Tyr Asp Pro Ala Lys Gln Ala Val Asp Ala Leu Phe Thr Asn Ser Gln
290 295 300
Gly Glu Glu Leu His Tyr His Ile Thr Leu Asp His Ile Gln Asn Ala
305 310 315 320
Asn Gln Leu Val Gln Ser Ile Pro Tyr Val His His Ala Trp Leu Pro
325 330 335
Asp Ala Pro Gly Met Asn Tyr Asp Leu Tyr Asn Asn Leu Lys Val Arg
340 345 350
Ile Glu Gln Ala Arg Tyr Leu Tyr Asp Ala Arg Asn Val Ile Thr Asn
355 360 365
Gly Asp Phe Ala Gln Gly Leu Thr Gly Trp His Ala Thr Gly Lys Val
370 375 380
Asp Val Gln Gln Met Asp Gly Ala Ser Val Leu Val Leu Ser Asn Trp
385 390 395 400
Ser Ala Gly Val Ser Gln Asn Leu His Ala Gln Asp His His Gly Tyr
405 410 415
Met Leu Arg Val Ile Ala Lys Lys Glu Gly Pro Gly Lys Gly Tyr Val
420 425 430
Thr Met Met Asp Cys Asn Gly His Gln Glu Thr Leu Lys Phe Thr Ser
435 440 445
Cys Glu Glu Gly Tyr Met Thr Lys Thr Val Glu Val Phe Pro Glu Ser
450 455 460
Asp Arg Val Arg Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Tyr Ile
465 470 475 480
Asp Ser Ile Glu Leu Leu Cys Met Gln Gly Tyr Ala Ser Asn Asn Thr
485 490 495
Pro His Thr Gly Asn Met Tyr Glu Gln Ser Tyr Asn Gly Ile Tyr Asn
500 505 510
Gln Asn Thr Ser Asp Leu Tyr His Gln Gly Tyr Thr Asn Asn Tyr Asn
515 520 525
Gln Glu Ser Ser Ser Met Tyr Asn Gln Asn Tyr Thr Asn Asn Asp Asp
530 535 540
Gln His Ser Gly Cys Thr Cys Asn Gln Gly His Asn Ser Gly Cys Thr
545 550 555 560
Cys Asn Gln Gly Tyr Asn Arg
565
<210> 41
<211> 640
<212> PRT
<213> 苏云金芽孢杆菌
<400> 41
Met Glu Glu Leu Glu Leu Lys Arg Thr Asn Thr Leu Ser Ser Glu Asp
1 5 10 15
Val Asn Ile Leu Gln Ile Glu Asn Leu Val Lys Glu Tyr Val Lys Gln
20 25 30
Thr Tyr Gly Asn Ser Ala Glu Ile Lys Lys Leu Ser Leu Asp Gly Leu
35 40 45
Asp Val Leu Tyr Asn Leu Asp Ile Pro Ser Ile Leu Lys Gly Thr Ser
50 55 60
Ser Ser Ser Ala Ile Lys Val Gly Thr Asp Asn Leu Asn Asn Pro Thr
65 70 75 80
Asp Thr Ala Lys Thr Ile Lys Leu Pro Val Lys Asn Val Arg Lys Lys
85 90 95
Glu Phe Lys Val Lys Pro Ile Gln Ala Leu Asn Phe Glu Asn Gly Ala
100 105 110
Thr Ile Thr Lys Lys Ser Ile Thr Ser Ile Pro Ser Ile Asn Ala Thr
115 120 125
Phe Ile Ala Leu Ala Glu Gln Asn Phe Gln Asn Ala His Phe His Ile
130 135 140
Val Asn Asp Ser Gln Ser Tyr Glu Asn Glu Ile Pro Ile Tyr Val Pro
145 150 155 160
Pro His Ser Lys Val Glu Ile Thr Tyr Tyr Val Lys Glu Ile Gln Phe
165 170 175
Asp Ala Ile Ile Gln Ser Thr Ala Thr Ile Gly Gly Ser Ile Ser Phe
180 185 190
Glu Tyr Ile Val His Asp Asn Gly His Glu Gly Ile Asp Phe Leu Thr
195 200 205
Ile Phe Glu Leu Val Asn Ser Leu Asn Leu Asn Asp Phe Glu Ile Gln
210 215 220
Glu Ala Ser Asp Val His Gly Lys Val Val Tyr Lys Gly Lys Ser Gln
225 230 235 240
Phe Gln Gly Thr Val Gly Leu Asn Leu Phe Met Gln Ile Lys Gly Thr
245 250 255
Pro Leu Asp Glu Ser Lys Asn Asn Tyr Glu Phe Thr Lys Val Leu Ser
260 265 270
Glu Asp Val Glu Met Ser Leu Ser Pro Ser Glu Gly Glu Tyr Tyr Ile
275 280 285
Asp Phe Gly Ser Ser Pro Lys Leu Thr Asn Lys Glu Glu Val Ile Val
290 295 300
Lys Phe Thr Arg Asp Tyr Leu Leu Ser Asn Asp Arg Lys Asn Ala Tyr
305 310 315 320
Val Gln Gln Leu Pro Arg Leu Glu Tyr Gly Glu Glu Val Thr Thr Leu
325 330 335
Lys Ser Ile Asp Thr Ala His Glu Arg Lys Glu Ile Ile Ala Ser Thr
340 345 350
Ile Asn Thr Phe Gln Asn Pro Ser Asp Thr Glu Ile Thr Arg Asn Thr
355 360 365
Ile Lys Glu Thr Phe Ser Thr Thr Asp Thr Ile Thr Thr Thr Ala Thr
370 375 380
Thr Asp Lys Phe Leu Glu Leu Gly Gly Ser Ile Glu Thr Ser Ala Lys
385 390 395 400
Gly Lys Val Pro Leu Val Ala Glu Ala Ser Ile Lys Val Thr Gln Ser
405 410 415
Ile Lys Gly Gly Trp Lys Trp Val Ser Thr Lys Thr Asn Thr Arg Thr
420 425 430
Asn Val His Thr Ile Glu Ile Pro Ser Gln Ser Ile Lys Ile Pro Pro
435 440 445
His Lys Met Trp Lys Tyr Gln Tyr Ile Leu Thr Lys Phe Glu Ser Ser
450 455 460
Gly Tyr Leu Ser Ser Ala Trp Glu Ile Asn Thr Lys Glu Ser Met Ser
465 470 475 480
Ala Pro Glu Val His Ile Gly Tyr Tyr Asn Lys Asp Leu Gln Asn Pro
485 490 495
Arg Asn Ile Thr Gly Leu Ser Ala Asn Val Glu Ser Gly Asn Val Val
500 505 510
Gly Arg Val Phe Glu Phe Asn Lys Phe Gln Pro Gly Gly Leu His Tyr
515 520 525
Lys Ile Leu Asn Ser Glu Asn Ile Leu Asn Ala Thr Pro Tyr Gln Phe
530 535 540
Phe Lys Glu Leu Ala Lys Arg Val Asn Gln Tyr Pro Leu Ile Gln Asn
545 550 555 560
Asn Pro Arg Tyr Arg Arg Leu Gly Ile Leu Leu Gly Phe Gly Lys Asp
565 570 575
Ile Ser Gln Ile Thr Trp Glu Pro Gln Ile His Tyr Asn Glu His Val
580 585 590
Leu Phe Asp Ala Glu Glu Leu Leu Asn Val Leu Arg Phe Asp Asp Ile
595 600 605
Ala Asn Lys Val Tyr Ala Leu Asp Gly Gly Thr Pro Phe Thr Val Ala
610 615 620
Val Gly His Glu Leu Leu Pro Lys Glu Ser Ile Glu Pro Leu Asn Asn
625 630 635 640
<210> 42
<211> 966
<212> PRT
<213> 苏云金芽孢杆菌
<400> 42
Met Met Asn Met Ser Asn Thr Leu Ala Pro Tyr Asn Val Leu Arg Ser
1 5 10 15
Met Asp Met Pro Asn Ile Ser Gly Thr Lys Trp Asp Lys Gly Met Phe
20 25 30
Ile Asn Ala Leu Asp Asn Thr Ser Phe Leu Leu Glu Leu Ile Glu Lys
35 40 45
Gly Ile Asn Asp Asp Asp Asp Val Leu Gly Leu Leu Ser Phe Ile Gly
50 55 60
Leu Thr Ala Leu Glu Ala Ile Pro Ile Val Gly Gly Val Met Ser Lys
65 70 75 80
Leu Val Ser Met Ile Phe Phe Pro Thr Lys Ser Ser Ile Asn Phe Gln
85 90 95
Lys Ile Trp Glu Gln Leu Glu Lys Ala Ile Glu Gln Ile Val Asp Lys
100 105 110
Lys Ile Thr Glu Ala Met Met Ser Gln Leu Met Gln Glu Ile Ala Gly
115 120 125
Leu Ala Asp Val Leu Glu Glu Tyr Arg Asn Ala Tyr Asp Leu Tyr Asn
130 135 140
Gly Lys Lys Leu Phe Asn Ile Pro Asp Lys Met Thr Pro Gly Asp Tyr
145 150 155 160
Leu Ile Asn Val Phe Thr Thr Ala Asn Leu Gln Phe Ile Gln Arg Ile
165 170 175
Pro Thr Phe Gln Asn Ser Ile Tyr Asp Val Val Phe Leu Pro Phe Phe
180 185 190
Val His Ala Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala Ile
195 200 205
His Gly Gln Glu Trp Gly Met Asp Glu Thr Val His Gln Lys Phe Lys
210 215 220
Arg Asp Leu Lys Thr Leu Ile Asn Lys Tyr Ser Ser Tyr Leu Leu Ala
225 230 235 240
Thr Tyr Lys Lys Gly Leu Lys Glu Ala Ser Glu Lys Lys Leu Glu Asn
245 250 255
Asn Asp Phe Pro Thr Ser Asn Asn Gln His His Tyr Ile Asn Thr Val
260 265 270
Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Ala Leu Thr Val
275 280 285
Phe Asp Phe Ala Tyr Lys Trp Lys Tyr Tyr Gln Glu Val Tyr Gln Asn
290 295 300
Asn Ile Thr Leu Asn Pro Ala Arg Thr Ile Tyr Ser Asp Ile Ala Gly
305 310 315 320
Ser Val Tyr Pro Tyr Glu Lys Thr Thr Asn Glu Ile Asp Asn Ile Ile
325 330 335
Lys Glu Gln Asn Leu Lys Tyr Arg Gly Leu Leu Lys Glu Leu Leu Ile
340 345 350
Asn His Gly Asp Arg Ile Asp Ser Ile Gln Ser Lys Tyr Ile Arg Asn
355 360 365
Asn Glu Ile Ile Asp Ser Asn Arg Thr Gly Gly Ala Gly Gly Arg Ala
370 375 380
Thr Phe Phe Asp Leu Lys Ser Pro Ile Asn Asn Pro Phe Ile Gln Val
385 390 395 400
Asn Met Trp Ser Glu Leu Val Pro Phe Ser Leu Gly Phe Lys Tyr Tyr
405 410 415
Asn Gly Glu Glu Ser Lys Leu Ile Trp Gly Gly Gly Thr Pro Gly Lys
420 425 430
His Lys Phe Gly Ser Tyr His Tyr Val Gly Asn Lys Val Ser Ser Ile
435 440 445
Ile Gly Phe Gly Lys Asn Gly Thr Gly Gly Phe Asn Ser Leu Asp Ala
450 455 460
Met Val Val Gly Phe Lys Arg Asp Asp Tyr Ile Pro Glu Asn Arg Phe
465 470 475 480
Val Gly Val Asn Lys Asn Gly Glu Pro Val Thr Lys Val Ile Asp Ala
485 490 495
Glu Asn Phe Tyr Gln Glu Lys Phe Gln Ser Asn Ile Lys Met Ile Asp
500 505 510
Glu Pro Met Phe Gly Glu Ala Val Leu Gln Phe Glu Asn Tyr Ser Asn
515 520 525
Asn Leu Asn Lys Asp Ser Tyr Val Thr Tyr Gln Ile Asp Ala Lys Ile
530 535 540
Glu Gly Thr Tyr Glu Leu His Val Ile Ile Gly Ala Lys Lys Gln Lys
545 550 555 560
Asp Lys Ile Ala Phe Lys Met Ala Leu Asn Glu Lys Gln Pro Glu Lys
565 570 575
Phe Ile Thr Glu Pro Phe Asn Ala Gly Asp Ile Trp Glu Gly Ile Ser
580 585 590
Leu Ser Glu Gly Leu Val Tyr Lys Arg Ile Leu Leu Gly Asn Phe Gln
595 600 605
Leu Lys Lys Gly Met Asn Arg Ile Thr Ile His Asn Gly Val Leu Gln
610 615 620
Thr Ser Ala Asn Ile Lys Thr Trp Asn Leu Ala Lys Leu Glu Leu Thr
625 630 635 640
Leu Thr Ser Asp Ser Leu Lys Asp Pro Asp Ile Thr Thr Leu Tyr Asp
645 650 655
Lys Asp Asn Tyr Ser Gly Thr Lys Lys Phe Ile Phe Glu Asn Thr Ser
660 665 670
Arg Leu Lys Asp Phe Asn Asp Lys Thr Ser Ser Ile Lys Val Glu Ser
675 680 685
His Leu Ala Gly Ile Arg Ile Tyr Gln Asp Tyr Asn Tyr Lys Gly Lys
690 695 700
Ser Met Asp Leu Val Gly Gly Glu Lys Ile Ser Leu Lys Asn His Ser
705 710 715 720
Phe Asn Asn Arg Ala Ser Ser Val Lys Phe Ala Asn Ile Val Leu Tyr
725 730 735
Asn Gln Asp Asn Tyr Gln Gly Ser Arg Lys Leu Val Phe Glu Asp Ile
740 745 750
Pro Asp Leu Gly Lys Gln Ser Phe Asn Asp Lys Thr Ser Ser Ile Val
755 760 765
Val Ser Ser Asn Val Ser Gly Ala Arg Leu Tyr Glu His Ala Tyr Tyr
770 775 780
Lys Gly Lys Tyr Val Asp Val Val Gly Gly Gln Lys Leu Asn Leu Lys
785 790 795 800
Asn His Val Leu Asn Lys Lys Ile Ser Ser Ile Lys Phe Phe Lys Glu
805 810 815
Gly Glu Val Leu Asn Gly Val Tyr Gln Ile Ile Thr Ala Ile Asn Asn
820 825 830
Thr Ser Val Ile Asp Lys His Leu Glu Asn Ser Asn Val His Leu Trp
835 840 845
Glu Asn Ala Glu Asn Lys Asn Gln Lys Trp Arg Ile Glu Tyr Asp Val
850 855 860
Ala Lys Lys Ala Tyr Gln Ile Lys Asn Met Leu Asp Glu Lys Leu Val
865 870 875 880
Leu Ser Thr His Glu Leu Phe Pro Ile Phe Ser Ala Leu Tyr Cys Leu
885 890 895
Pro Asn Lys Gly Tyr Val Ser Gln Tyr Trp Ile Phe Glu Tyr Val Gly
900 905 910
Asn Gly Tyr Tyr Ile Ile Lys Asn Lys Ala Tyr Pro Asp Trp Val Leu
915 920 925
Asp Val Asp Gly Leu Asn Ser Asp Asn Gly Thr Leu Ile Lys Leu His
930 935 940
Ser Gln His Asp Leu Thr Asp Pro Leu Ile Asn Ala Gln Lys Phe Lys
945 950 955 960
Leu Lys Asp Ile Asn Asn
965
<210> 43
<211> 769
<212> PRT
<213> 苏云金芽孢杆菌
<400> 43
Met Met Asn Met Ser Asn Thr Leu Ala Pro Tyr Asn Val Leu Arg Ser
1 5 10 15
Met Asp Met Pro Asn Ile Ser Gly Thr Lys Trp Asp Lys Gly Met Phe
20 25 30
Ile Asn Ala Leu Asp Asn Thr Ser Phe Leu Leu Glu Leu Ile Glu Lys
35 40 45
Gly Ile Asn Asp Asp Asp Asp Val Leu Gly Leu Leu Ser Phe Ile Gly
50 55 60
Leu Thr Ala Leu Glu Ala Ile Pro Ile Val Gly Gly Val Met Ser Lys
65 70 75 80
Leu Val Ser Met Ile Phe Phe Pro Thr Lys Ser Ser Ile Asn Phe Gln
85 90 95
Lys Ile Trp Glu Gln Leu Glu Lys Ala Ile Glu Gln Ile Val Asp Lys
100 105 110
Lys Ile Thr Glu Ala Met Met Ser Gln Leu Met Gln Glu Ile Ala Gly
115 120 125
Leu Ala Asp Val Leu Glu Glu Tyr Arg Asn Ala Tyr Asp Leu Tyr Asn
130 135 140
Gly Lys Lys Leu Phe Asn Ile Pro Asp Lys Met Thr Pro Gly Asp Tyr
145 150 155 160
Leu Ile Asn Val Phe Thr Thr Ala Asn Leu Gln Phe Ile Gln Arg Ile
165 170 175
Pro Thr Phe Gln Asn Ser Ile Tyr Asp Val Val Phe Leu Pro Phe Phe
180 185 190
Val His Ala Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala Ile
195 200 205
His Gly Gln Glu Trp Gly Met Asp Glu Thr Val His Gln Lys Phe Lys
210 215 220
Arg Asp Leu Lys Thr Leu Ile Asn Lys Tyr Ser Ser Tyr Leu Leu Ala
225 230 235 240
Thr Tyr Lys Lys Gly Leu Lys Glu Ala Ser Glu Lys Lys Leu Glu Asn
245 250 255
Asn Asp Phe Pro Thr Ser Asn Asn Gln His His Tyr Ile Asn Thr Val
260 265 270
Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Ala Leu Thr Val
275 280 285
Phe Asp Phe Ala Tyr Lys Trp Lys Tyr Tyr Gln Glu Val Tyr Gln Asn
290 295 300
Asn Ile Thr Leu Asn Pro Ala Arg Thr Ile Tyr Ser Asp Ile Ala Gly
305 310 315 320
Ser Val Tyr Pro Tyr Glu Lys Thr Thr Asn Glu Ile Asp Asn Ile Ile
325 330 335
Lys Glu Gln Asn Leu Lys Tyr Arg Gly Leu Leu Lys Glu Leu Leu Ile
340 345 350
Asn His Gly Asp Arg Ile Asp Ser Ile Gln Ser Lys Tyr Ile Arg Asn
355 360 365
Asn Glu Ile Ile Asp Ser Asn Arg Thr Gly Gly Ala Gly Gly Arg Ala
370 375 380
Thr Phe Phe Asp Leu Lys Ser Pro Ile Asn Asn Pro Phe Ile Gln Val
385 390 395 400
Asn Met Trp Ser Glu Leu Val Pro Phe Ser Leu Gly Phe Lys Tyr Tyr
405 410 415
Asn Gly Glu Glu Ser Lys Leu Ile Trp Gly Gly Gly Thr Pro Gly Lys
420 425 430
His Lys Phe Gly Ser Tyr His Tyr Val Gly Asn Lys Val Ser Ser Ile
435 440 445
Ile Gly Phe Gly Lys Asn Gly Thr Gly Gly Phe Asn Ser Leu Asp Ala
450 455 460
Met Val Val Gly Phe Lys Arg Asp Asp Tyr Ile Pro Glu Asn Arg Phe
465 470 475 480
Val Gly Val Asn Lys Asn Gly Glu Pro Val Thr Lys Val Ile Asp Ala
485 490 495
Glu Asn Phe Tyr Gln Glu Lys Phe Gln Ser Asn Ile Lys Met Ile Asp
500 505 510
Glu Pro Met Phe Gly Glu Ala Val Leu Gln Phe Glu Asn Tyr Ser Asn
515 520 525
Asn Leu Asn Lys Asp Ser Tyr Val Thr Tyr Gln Ile Asp Ala Lys Ile
530 535 540
Glu Gly Thr Tyr Glu Leu His Val Ile Ile Gly Ala Lys Lys Gln Lys
545 550 555 560
Asp Lys Ile Ala Phe Lys Met Ala Leu Asn Glu Lys Gln Pro Glu Lys
565 570 575
Phe Ile Thr Glu Pro Phe Asn Ala Gly Asp Ile Trp Glu Gly Ile Ser
580 585 590
Leu Ser Glu Gly Leu Val Tyr Lys Arg Ile Leu Leu Gly Asn Phe Gln
595 600 605
Leu Lys Lys Gly Met Asn Arg Ile Thr Ile His Asn Gly Val Leu Gln
610 615 620
Thr Ser Ala Asn Ile Lys Thr Trp Asn Leu Ala Lys Leu Glu Leu Thr
625 630 635 640
Leu Thr Ser Asp Ser Leu Lys Asp Pro Asp Ile Thr Thr Leu Tyr Asp
645 650 655
Lys Asp Asn Tyr Ser Gly Thr Lys Lys Phe Ile Phe Glu Asn Thr Ser
660 665 670
Arg Leu Lys Asp Phe Asn Asp Lys Thr Ser Ser Ile Lys Val Glu Ser
675 680 685
His Leu Ala Gly Ile Arg Ile Tyr Gln Asp Tyr Asn Tyr Lys Gly Lys
690 695 700
Ser Met Asp Leu Val Gly Gly Glu Lys Ile Ser Leu Lys Asn His Ser
705 710 715 720
Phe Asn Asn Arg Ala Ser Ser Val Lys Phe Ala Asn Ile Val Leu Tyr
725 730 735
Asn Gln Asp Asn Tyr Gln Gly Ser Arg Lys Leu Val Phe Glu Asp Ile
740 745 750
Pro Asp Leu Gly Lys Gln Ser Phe Asn Asp Lys Thr Ser Ser Ile Val
755 760 765
Val
<210> 44
<211> 575
<212> PRT
<213> 苏云金芽孢杆菌
<400> 44
Met Asn Gly Asn Gly Arg His Asp Gly Trp Asn Gln Asn Gln His Ile
1 5 10 15
Glu Asn Gly Gln Met Asn Pro Asn His Ser Gly Ser Cys Lys Cys Gly
20 25 30
Cys Gln Gln Asn Asn Asn Gly Ser Tyr Pro Ser Asn Glu Tyr Asn Ser
35 40 45
Asn Asn Asn Gly Ser Tyr Pro Ser Asn Glu Tyr Asn Ser Asn Asn Asn
50 55 60
Gly Ser Tyr Pro Ser Asn Glu Tyr Asn Ser Asn Asn Asn Gly Ser Tyr
65 70 75 80
Pro Ser Asn Glu Tyr Asn Ser Asn Asn Asn Gly Ser Tyr Pro Ser Asn
85 90 95
Glu Tyr Asn Ser Asn Asn Asn Gly Ser Tyr Pro Ser Asn Glu Tyr Asn
100 105 110
Ser Asn Asn Asn Gly Ser Tyr Pro Ser Asn Glu Tyr Asn Ser Asn Asn
115 120 125
Asn Gly Ser Tyr Pro Ser Asn Glu Tyr Asn Ser Asn Asn Asn Gly Ser
130 135 140
Tyr Ser Ser Asn Glu Tyr Asn Ser Asn Asn Asn Gly Ser Tyr Pro Ser
145 150 155 160
Asn Glu Tyr Asn Ser Asn Asn Asn Gly Ser Tyr Pro Ser Asn Glu Tyr
165 170 175
Asn Ser Asn Asn Asn Gly Ser Tyr Pro Ser Asn Glu Tyr Val Gly Gly
180 185 190
Tyr Ser Ile Gln Asp Gly Leu Pro Gln Glu Ser Lys Gln Phe Gln Lys
195 200 205
Ile Ser Asn Met Asn Thr Arg Asp Asn His Arg Val Leu Asp Ala Gln
210 215 220
Asp Thr Tyr Phe Gly Gln Leu Ile Asp Asn Arg Val Gly Asp Thr Cys
225 230 235 240
Lys Tyr Val Glu His Lys Asn Ser Val Ile Tyr Glu Leu Ser Arg Gln
245 250 255
Pro Val Tyr Thr Pro Asp Ser Gln Tyr Phe Ile Phe Tyr Gln Met Asp
260 265 270
Asn Gly Asn Phe Ile Ile Ala Asn Lys Glu Asn Ser Arg Val Leu Glu
275 280 285
Val Ile Phe Ser Ser Val Asn Gly Phe Val Thr Ile Ser Asn Glu Phe
290 295 300
Asn Ala Thr Ser Asp Gln Arg Phe Lys Val Val Arg Ser Lys Asn Asp
305 310 315 320
Thr Phe Arg Leu Val Thr Glu Gly Asn Lys Thr Leu Asn Ile Cys Gly
325 330 335
His Ser Phe Gln Tyr Asn Thr Lys Ile Thr Ala Val Asn Ala Asp Ile
340 345 350
Asp Gly Asp Asn Tyr Leu Phe Gln Lys Ser Met Asp Lys Asp Thr Arg
355 360 365
Asp Leu Tyr Phe Gly Thr Ile Ser Asn Lys Asn Pro Glu Ile Leu Asn
370 375 380
Asp Pro Arg Asn Leu Lys Ser Leu Asp Asp Leu Gly Asp Glu Pro Arg
385 390 395 400
Ala Phe Lys Gly Ala Ala Leu Leu Pro Ala Leu Phe Val Asn Asp Pro
405 410 415
Arg Tyr Ser Val His Arg Arg Val Ser Asn Ser Pro Tyr Tyr Tyr Leu
420 425 430
Glu Tyr Thr Gln Tyr Trp His Arg Ile Trp Thr Asp Val Leu Pro Ile
435 440 445
Asp Gly Tyr Gly Ala Trp Ile Glu Met Ile Gly Val Thr Asn Asp Thr
450 455 460
Gln Val Asn Met Lys Asn Ile Met Asn Ile Thr Ile Thr Gly Lys Asp
465 470 475 480
Leu Gly Val Asp Leu Gly Ile Asp Leu Gly Leu Arg Phe Gly Asp Lys
485 490 495
Ser Phe Leu Phe Glu Gln Lys Ile Leu Ser Gly Leu Ser Ile Arg Lys
500 505 510
Thr Asp Tyr Pro Asn Leu Gly Ile Asp Glu Arg Ala Met Tyr Gln Arg
515 520 525
Asn Asn Ser Asn Leu Lys Thr Arg Phe Val Arg Tyr Val Lys Lys His
530 535 540
Glu Phe Val Leu Arg Asp Leu Asn Gly Ser Lys Val Ala Glu Pro Trp
545 550 555 560
Ile Ile Thr Glu Asp Arg Ser Ile Thr Lys Glu Tyr Ser Ser Asn
565 570 575
<210> 45
<211> 559
<212> PRT
<213> 苏云金芽孢杆菌
<400> 45
Met Lys Tyr Lys Asn Arg Thr Arg Ala Lys Cys Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Val Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Asn Ala Ser Ala Phe Ala Asp Glu Lys Glu Lys Asn Val Ile Gln
35 40 45
Gln Lys Ser Pro Gly Thr Tyr Tyr Glu Asp Ala Gln Lys Asn Leu Gly
50 55 60
Ser Leu Ala Arg Phe Asp Thr Trp Ala Gln Asp Leu Gly Lys Thr Thr
65 70 75 80
Gly Ala Gly Asn Tyr Lys Thr Thr Leu Gly Met Ala Glu Lys Leu Leu
85 90 95
Pro Thr Ile Tyr Asn Asp Leu Asn Ser Gly Asn Phe Asn Asn Thr Ala
100 105 110
Arg Ser Ile Thr Met Leu Ser Thr Ala Leu Ile Pro Tyr Gly Gly Ala
115 120 125
Phe Ile Ser Pro Ile Ile Gly Ile Leu Trp Pro Glu Asn Gly Pro Asn
130 135 140
Ile Lys Glu Met Leu Gln Glu Met Glu Asn Lys Leu Val Gly Ile Met
145 150 155 160
Asp Glu Lys Ile Glu Ala Lys Asp Leu Asp Asp Leu Glu Ala Ala Val
165 170 175
Lys Gly Leu Met Val Ser Leu Lys Glu Phe Glu Asn Ser Leu Asn Gly
180 185 190
Asn Ile Gly Gly Glu Tyr Tyr Ser Ala Leu Ala Asp Val Asp Ser Leu
195 200 205
Asn Arg Gly Arg Ile Thr Ala Ile Gln Lys Gly Phe Asn Asp Leu Ile
210 215 220
Ser Ala Thr Ser Lys Pro Lys Phe Lys Ile Thr Glu Leu Pro Leu Tyr
225 230 235 240
Thr Ile Ile Ala Thr Ala His Leu Asn Phe Leu Asn Thr Val Glu Lys
245 250 255
Gln Gly Thr Ser Pro Lys Ile Asn Tyr Thr Glu Ala Ala Leu Lys Asp
260 265 270
Leu Leu Gln Asn Met Lys Lys Asn His Lys Asp Tyr Ala Asp Tyr Ile
275 280 285
Glu Lys Thr Tyr Thr Glu Gly Glu Ala Arg Ile Asn Ser Lys Leu Glu
290 295 300
Asp Lys Gln Lys Ile Glu Gln Asp Leu Ala Ala Val Asn Gln Lys Leu
305 310 315 320
Ser Glu Met Pro Arg Lys Pro Lys Asn His Thr His Glu Glu Glu Asn
325 330 335
Lys Phe Ile Ile Gln Lys Glu Lys Leu Tyr Ala Gln Gln Asp Ser Leu
340 345 350
Glu Lys Lys Leu Ser Glu Tyr Asn Asp Leu Met Tyr Gln Lys Ser Asp
355 360 365
Phe Tyr Ser Lys Thr Lys Gly Ser Glu Ala Phe Gln Ile Ala Ser Thr
370 375 380
Gly Lys Thr Ile Pro Thr Pro Ser Trp Val Lys Thr Glu Gly Thr Trp
385 390 395 400
Val Cys Glu Ala Gly Phe Trp Phe Tyr Ile Asp Ala Lys Gly Gln Lys
405 410 415
Lys Ser Asp Trp Phe Asn Asp Lys Thr Pro Asp Gly Lys Asp Arg Trp
420 425 430
Tyr Tyr Leu Ser Thr Glu Thr Pro Arg Leu Asp Asn Val Arg Gly Asn
435 440 445
Ala Tyr Val Gly Lys Gly Thr Met Leu Thr Gly Trp Phe His Asp Thr
450 455 460
Arg Lys Asp Lys Gln Ile Ile Gly Val Asn Thr Lys Thr Thr Tyr Glu
465 470 475 480
Tyr Trp Tyr Tyr Leu Ser Pro Glu Lys Asn Leu Lys Asn Ser Ala Gly
485 490 495
Glu Leu Phe Lys Gln Gly Gln Met Met Thr Lys Trp Val Glu Ile Lys
500 505 510
Asp Thr Lys Thr Gly Glu Pro His Trp Tyr Tyr Phe Asn Pro Asp Asp
515 520 525
Gly Ser Met Thr His Asp Lys Lys Ala Val Gln Ile Gly Asp Lys Lys
530 535 540
Tyr Asp Phe Gly Ser Asn Gly Val Cys Thr Thr Pro Asn Gly Tyr
545 550 555
<210> 46
<211> 1293
<212> PRT
<213> 侧孢短芽孢杆菌
<400> 46
Met Asn Gln Asn Gln Asn Lys Asn Glu Met Gln Ile Ile Glu Pro Ser
1 5 10 15
Ser Asp Ser Phe Leu Tyr Ser His Asn Asn Tyr Pro Tyr Ala Thr Asp
20 25 30
Pro Asn Thr Val Leu Glu Gly Arg Asn Tyr Lys Glu Trp Leu Asn Lys
35 40 45
Cys Thr Asp Asn Tyr Thr Asp Ala Leu Gln Ser Pro Glu Ala Thr Ala
50 55 60
Ile Ser Lys Gly Ala Val Ser Ala Ala Ile Ser Ile Ser Thr Lys Val
65 70 75 80
Leu Gly Leu Leu Gly Val Pro Phe Ala Ala Gln Ile Gly Gln Leu Trp
85 90 95
Thr Phe Ile Leu Asn Ala Leu Trp Pro Ser Asp Asn Thr Gln Trp Glu
100 105 110
Glu Phe Met Arg His Val Glu Glu Leu Ile Asn Gln Arg Ile Ala Asp
115 120 125
Tyr Ala Arg Asn Lys Ala Leu Ala Glu Leu Thr Gly Leu Gly Asn Asn
130 135 140
Leu Asp Leu Tyr Ile Glu Ala Leu Asp Asp Trp Lys Arg Asn Pro Thr
145 150 155 160
Ser Gln Glu Ala Lys Thr Arg Val Ile Asp Arg Phe Arg Ile Val Asp
165 170 175
Gly Leu Phe Glu Ala Tyr Ile Pro Ser Phe Ala Val Ser Gly Tyr Gln
180 185 190
Val Gln Leu Leu Thr Val Tyr Ala Ala Ala Ala Asn Leu His Leu Leu
195 200 205
Leu Leu Arg Asp Ser Thr Ile Tyr Gly Ile Asp Trp Gly Leu Ser Gln
210 215 220
Thr Asn Val Asn Asp Asn Tyr Asn Arg Gln Ile Arg Leu Thr Ala Thr
225 230 235 240
Tyr Ala Asn His Cys Thr Thr Trp Tyr Gln Thr Gly Leu Glu Arg Leu
245 250 255
Arg Gly Ser Asn Ala Ser Ser Trp Val Thr Tyr Asn Arg Phe Arg Arg
260 265 270
Glu Met Thr Leu Thr Val Leu Asp Ile Cys Ser Leu Phe Ser Asn Tyr
275 280 285
Asp Tyr Arg Ser Tyr Pro Ala Glu Val Arg Gly Glu Ile Thr Arg Glu
290 295 300
Ile Tyr Thr Asp Pro Val Gly Val Gly Trp Val Asp Ser Ala Pro Ser
305 310 315 320
Phe Gly Glu Ile Glu Asn Leu Ala Ile Arg Ala Pro Arg Thr Val Thr
325 330 335
Trp Leu Asn Ser Thr Arg Ile Phe Thr Gly Arg Leu Gln Gly Trp Ser
340 345 350
Gly Thr Asn Asn Tyr Trp Ala Ala His Met Gln Asn Phe Ser Glu Thr
355 360 365
Asn Ser Gly Asn Ile Gln Phe Glu Gly Pro Leu Tyr Gly Ser Thr Val
370 375 380
Gly Thr Ile His Arg Thr Asp Asp Tyr Asp Met Gly Asn Arg Asp Ile
385 390 395 400
Tyr Thr Ile Thr Ser Gln Ala Val Leu Gly Leu Trp Ala Thr Gly Gln
405 410 415
Arg Val Leu Gly Val Ala Ser Ala Arg Phe Thr Leu Arg Asn Leu Phe
420 425 430
Asn Asn Leu Thr Gln Val Leu Val Tyr Glu Asn Pro Ile Ser Ser Thr
435 440 445
Phe Gly Ser Ser Thr Leu Thr His Glu Leu Ser Gly Glu Asn Ser Asp
450 455 460
Arg Pro Thr Ser Ser Asp Tyr Ser His Arg Leu Thr Ser Ile Thr Gly
465 470 475 480
Phe Arg Ala Gly Ala Asn Gly Thr Val Pro Val Phe Gly Trp Thr Ser
485 490 495
Ala Thr Val Asp Arg Asn Asn Ile Ile Glu Arg Asn Lys Ile Thr Gln
500 505 510
Phe Pro Gly Val Lys Ser His Thr Leu Asn Asn Cys Gln Val Val Arg
515 520 525
Gly Thr Gly Phe Thr Gly Gly Asp Trp Leu Arg Pro Asn Asn Asn Gly
530 535 540
Thr Phe Arg Leu Thr Ile Thr Ser Phe Ser Ser Gln Ser Tyr Arg Ile
545 550 555 560
Arg Leu Arg Tyr Ala Thr Ser Val Gly Asn Thr Ser Leu Val Ile Ser
565 570 575
Ser Ser Asp Ala Gly Ile Ser Ser Thr Thr Ile Pro Leu Thr Ser Thr
580 585 590
Ile Thr Ser Leu Pro Gln Thr Val Pro Tyr Gln Ala Phe Arg Val Val
595 600 605
Asp Leu Pro Ile Thr Phe Thr Thr Pro Thr Thr Gln Arg Asn Tyr Thr
610 615 620
Phe Asp Phe Arg Leu Gln Asn Pro Ser Asn Ala Asn Val Phe Ile Asp
625 630 635 640
Arg Phe Glu Phe Val Pro Ile Gly Gly Ser Leu Ser Glu Tyr Glu Thr
645 650 655
Lys His Gln Leu Glu Lys Ala Arg Lys Ala Val Asn Asp Leu Phe Thr
660 665 670
Asn Glu Ser Lys Asn Val Leu Lys Lys Asp Thr Thr Asp Tyr Asp Ile
675 680 685
Asp Gln Ala Ala Asn Leu Val Glu Cys Val Ser Asp Glu Cys Ala Asn
690 695 700
Ala Lys Met Ile Leu Leu Asp Glu Val Lys Tyr Ala Lys Gln Leu Ser
705 710 715 720
Glu Ala Arg Asn Leu Leu Leu Asn Gly Asn Phe Glu Tyr Gln Asp Arg
725 730 735
Asp Gly Glu Asn Pro Trp Lys Thr Ser Pro Asn Val Thr Ile Gln Glu
740 745 750
Asn Asn Pro Ile Phe Lys Gly Arg Tyr Leu Ser Met Ser Gly Ala Asn
755 760 765
Asn Ile Glu Ala Thr Asn Glu Ile Phe Pro Thr Tyr Val Tyr Gln Lys
770 775 780
Ile Asp Glu Ser Lys Leu Lys Pro Tyr Thr Arg Tyr Lys Val Arg Gly
785 790 795 800
Phe Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Thr Arg Tyr Asp
805 810 815
Glu Glu Val Asp Ala Ile Leu Asn Val Pro Asn Asp Ile Pro His Ala
820 825 830
Pro Pro Pro Phe Cys Gly Glu Phe Asp Arg Cys Lys Pro His Ser Tyr
835 840 845
Pro Pro Ile Asn Pro Glu Cys His His Asp Val Ile Asn Asn Ile Glu
850 855 860
Ile Ser Ser Pro Cys Gln His Asn Lys Met Val Asp Asn Ala Asp Ile
865 870 875 880
Ser Tyr Arg His Ser Arg Leu Ser Lys Lys His Gly Ile Cys His Glu
885 890 895
Ser His His Phe Glu Phe His Ile Asp Thr Gly Lys Ile Asp Leu Val
900 905 910
Glu Asn Leu Gly Ile Trp Val Val Phe Lys Ile Cys Ser Thr Asp Gly
915 920 925
Tyr Ala Thr Leu Asp Asn Leu Glu Val Ile Glu Glu Gly Pro Leu Gly
930 935 940
Ala Glu Ser Leu Glu Arg Val Lys Arg Arg Glu Lys Lys Trp Lys His
945 950 955 960
His Met Glu His Lys Cys Ser Glu Thr Lys His Ala Tyr His Ala Ala
965 970 975
Lys Gln Ala Val Val Ala Leu Phe Thr Asn Ser Lys Tyr Asp Arg Leu
980 985 990
Lys Phe Glu Thr Thr Ile Ser Asn Ile Leu Phe Ala Asp Tyr Leu Val
995 1000 1005
Gln Ser Ile Pro Tyr Val Tyr Asn Lys Trp Leu Pro Gly Val Pro Gly
1010 1015 1020
Met Asn Tyr Asp Ile Tyr Thr Glu Leu Lys Asn Leu Phe Thr Gly Ala
1025 1030 1035 1040
Phe Asn Leu Tyr Asp Gln Arg Asn Ile Ile Lys Asn Gly Asp Phe Asn
1045 1050 1055
Arg Gly Leu Met His Trp His Ala Thr Pro His Ala Arg Val Glu Gln
1060 1065 1070
Ile Ile Asp Asn Arg Ser Val Leu Val Leu Pro Asn Tyr Ala Ala Asn
1075 1080 1085
Val Ser Gln Glu Val Cys Leu Glu His Asn Arg Gly Tyr Val Leu Arg
1090 1095 1100
Val Thr Ala Lys Lys Glu Gly Pro Gly Ile Gly Tyr Val Thr Phe Ser
1105 1110 1115 1120
Asp Cys Ala Asn His Ile Glu Lys Leu Thr Phe Thr Ser Cys Asp Tyr
1125 1130 1135
Gly Thr Asn Val Val Pro Tyr Glu Gln Ser Asn Tyr Pro Thr Asp Gly
1140 1145 1150
Val Pro Tyr Gly Gln His Gly Cys Asn Ile Asp Gly Val Pro Tyr Glu
1155 1160 1165
Gln Ser Gly Tyr Arg Thr Asp Gly Val Pro Tyr Glu Gln Ser Gly Tyr
1170 1175 1180
Arg Thr Asp Gly Val Pro Tyr Glu Gln Ser Gly His Arg Thr Asp Gly
1185 1190 1195 1200
Val Pro Tyr Glu Gln Ser Gly Tyr Arg Thr Asp Gly Val Pro Cys Glu
1205 1210 1215
Gln His Gly Cys His Thr Asp Gly Leu Pro His Ile Gln His Gly Cys
1220 1225 1230
Arg Thr Asp Gly Leu Pro His Ile Gln His Gly Cys Arg Thr Asp Arg
1235 1240 1245
Ser Arg Asp Glu Leu Leu Gly Tyr Val Thr Lys Thr Ile Asp Val Phe
1250 1255 1260
Pro Asn Thr Asp Lys Val Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr
1265 1270 1275 1280
Phe Lys Val Glu Ser Val Glu Leu Ile Cys Met Glu Glu
1285 1290
<210> 47
<211> 647
<212> PRT
<213> 侧孢短芽孢杆菌
<400> 47
Met Asn Gln Asn Gln Asn Lys Asn Glu Met Gln Ile Ile Glu Pro Ser
1 5 10 15
Ser Asp Ser Phe Leu Tyr Ser His Asn Asn Tyr Pro Tyr Ala Thr Asp
20 25 30
Pro Asn Thr Val Leu Glu Gly Arg Asn Tyr Lys Glu Trp Leu Asn Lys
35 40 45
Cys Thr Asp Asn Tyr Thr Asp Ala Leu Gln Ser Pro Glu Ala Thr Ala
50 55 60
Ile Ser Lys Gly Ala Val Ser Ala Ala Ile Ser Ile Ser Thr Lys Val
65 70 75 80
Leu Gly Leu Leu Gly Val Pro Phe Ala Ala Gln Ile Gly Gln Leu Trp
85 90 95
Thr Phe Ile Leu Asn Ala Leu Trp Pro Ser Asp Asn Thr Gln Trp Glu
100 105 110
Glu Phe Met Arg His Val Glu Glu Leu Ile Asn Gln Arg Ile Ala Asp
115 120 125
Tyr Ala Arg Asn Lys Ala Leu Ala Glu Leu Thr Gly Leu Gly Asn Asn
130 135 140
Leu Asp Leu Tyr Ile Glu Ala Leu Asp Asp Trp Lys Arg Asn Pro Thr
145 150 155 160
Ser Gln Glu Ala Lys Thr Arg Val Ile Asp Arg Phe Arg Ile Val Asp
165 170 175
Gly Leu Phe Glu Ala Tyr Ile Pro Ser Phe Ala Val Ser Gly Tyr Gln
180 185 190
Val Gln Leu Leu Thr Val Tyr Ala Ala Ala Ala Asn Leu His Leu Leu
195 200 205
Leu Leu Arg Asp Ser Thr Ile Tyr Gly Ile Asp Trp Gly Leu Ser Gln
210 215 220
Thr Asn Val Asn Asp Asn Tyr Asn Arg Gln Ile Arg Leu Thr Ala Thr
225 230 235 240
Tyr Ala Asn His Cys Thr Thr Trp Tyr Gln Thr Gly Leu Glu Arg Leu
245 250 255
Arg Gly Ser Asn Ala Ser Ser Trp Val Thr Tyr Asn Arg Phe Arg Arg
260 265 270
Glu Met Thr Leu Thr Val Leu Asp Ile Cys Ser Leu Phe Ser Asn Tyr
275 280 285
Asp Tyr Arg Ser Tyr Pro Ala Glu Val Arg Gly Glu Ile Thr Arg Glu
290 295 300
Ile Tyr Thr Asp Pro Val Gly Val Gly Trp Val Asp Ser Ala Pro Ser
305 310 315 320
Phe Gly Glu Ile Glu Asn Leu Ala Ile Arg Ala Pro Arg Thr Val Thr
325 330 335
Trp Leu Asn Ser Thr Arg Ile Phe Thr Gly Arg Leu Gln Gly Trp Ser
340 345 350
Gly Thr Asn Asn Tyr Trp Ala Ala His Met Gln Asn Phe Ser Glu Thr
355 360 365
Asn Ser Gly Asn Ile Gln Phe Glu Gly Pro Leu Tyr Gly Ser Thr Val
370 375 380
Gly Thr Ile His Arg Thr Asp Asp Tyr Asp Met Gly Asn Arg Asp Ile
385 390 395 400
Tyr Thr Ile Thr Ser Gln Ala Val Leu Gly Leu Trp Ala Thr Gly Gln
405 410 415
Arg Val Leu Gly Val Ala Ser Ala Arg Phe Thr Leu Arg Asn Leu Phe
420 425 430
Asn Asn Leu Thr Gln Val Leu Val Tyr Glu Asn Pro Ile Ser Ser Thr
435 440 445
Phe Gly Ser Ser Thr Leu Thr His Glu Leu Ser Gly Glu Asn Ser Asp
450 455 460
Arg Pro Thr Ser Ser Asp Tyr Ser His Arg Leu Thr Ser Ile Thr Gly
465 470 475 480
Phe Arg Ala Gly Ala Asn Gly Thr Val Pro Val Phe Gly Trp Thr Ser
485 490 495
Ala Thr Val Asp Arg Asn Asn Ile Ile Glu Arg Asn Lys Ile Thr Gln
500 505 510
Phe Pro Gly Val Lys Ser His Thr Leu Asn Asn Cys Gln Val Val Arg
515 520 525
Gly Thr Gly Phe Thr Gly Gly Asp Trp Leu Arg Pro Asn Asn Asn Gly
530 535 540
Thr Phe Arg Leu Thr Ile Thr Ser Phe Ser Ser Gln Ser Tyr Arg Ile
545 550 555 560
Arg Leu Arg Tyr Ala Thr Ser Val Gly Asn Thr Ser Leu Val Ile Ser
565 570 575
Ser Ser Asp Ala Gly Ile Ser Ser Thr Thr Ile Pro Leu Thr Ser Thr
580 585 590
Ile Thr Ser Leu Pro Gln Thr Val Pro Tyr Gln Ala Phe Arg Val Val
595 600 605
Asp Leu Pro Ile Thr Phe Thr Thr Pro Thr Thr Gln Arg Asn Tyr Thr
610 615 620
Phe Asp Phe Arg Leu Gln Asn Pro Ser Asn Ala Asn Val Phe Ile Asp
625 630 635 640
Arg Phe Glu Phe Val Pro Ile
645
<210> 48
<211> 1256
<212> PRT
<213> 侧孢短芽孢杆菌
<400> 48
Met Asn Gln Asn Gln Asn Gln Asn Gln Asn Lys Asn Glu Leu Gln Ile
1 5 10 15
Ile Glu Pro Ser Ser Asp Ser Phe Leu Tyr Ser His Asn Asn Tyr Pro
20 25 30
Tyr Ala Thr Asp Pro Asn Thr Val Leu Gln Gly Arg Asn Tyr Lys Glu
35 40 45
Trp Leu Asn Met Cys Thr Gly Thr Asp Asp Ser Arg Ser Pro Glu Ala
50 55 60
Ala Ser Thr Ala Lys Ser Ala Ile Ser Val Ala Ile Thr Ile Ser Thr
65 70 75 80
Thr Ile Leu Gly Leu Leu Gly Val Pro Phe Ala Ser Gln Ile Gly Ala
85 90 95
Phe Tyr Asn Phe Val Leu Asn Thr Val Trp Pro Gln Gly Asn Asn Gln
100 105 110
Trp Glu Glu Phe Met Arg His Val Glu Asp Leu Ile Asn Glu Arg Ile
115 120 125
Ala Asp Tyr Ala Arg Ser Lys Ala Leu Ala Glu Leu Ala Gly Leu Gly
130 135 140
Asn Asn Leu Asp Leu Tyr Arg Glu Ala Phe Glu Asp Trp Arg Arg Asn
145 150 155 160
Pro Thr Ser Gln Gln Ala Lys Thr Arg Val Ile Glu Arg Phe Arg Ile
165 170 175
Leu Asp Gly Leu Phe Glu Gln Tyr Met Pro Ser Phe Ala Val Gln Gly
180 185 190
Phe Gln Val Gln Leu Leu Thr Val Tyr Ala Ser Ala Ala Asn Ile His
195 200 205
Leu Phe Leu Leu Arg Asp Ser Ser Ile Tyr Gly Leu Asp Trp Gly Leu
210 215 220
Ser Gln Thr Asn Val Asn Glu Asn Tyr Asn Arg Gln Ile Arg His Ala
225 230 235 240
Ala Thr Tyr Ala Asn His Cys Thr Thr Trp Tyr Gln Thr Gly Leu Gln
245 250 255
Arg Leu Gln Gly Thr Asn Ala Thr Ser Trp Val Ala Tyr Asn Arg Phe
260 265 270
Arg Arg Glu Met Thr Leu Thr Val Leu Asp Ile Ser Ser Leu Phe Ser
275 280 285
Asn Tyr Asp Tyr Arg Ser Tyr Pro Thr Glu Val Arg Gly Glu Leu Thr
290 295 300
Arg Glu Ile Tyr Thr Asp Pro Val Gly Arg Asn Trp Gln Asn Ser Ala
305 310 315 320
Pro Ser Phe Ala Gln Ile Glu Asn Leu Ala Ile Arg Ala Pro Arg Thr
325 330 335
Val Thr Trp Leu Asn Ser Thr Arg Ile Ser Thr Gly Thr Leu Gln Gly
340 345 350
Trp Ser Gly Ser Asn Arg Tyr Trp Ala Ala His Met Gln Asn Phe Ser
355 360 365
Glu Thr Asn Ser Gly Asn Ile Arg Phe Asp Gly Pro Leu Tyr Gly Ser
370 375 380
Thr Val Gly Thr Ile His Arg Thr Asp Asp Tyr Asp Met Gly Asn Arg
385 390 395 400
Asp Ile Tyr Thr Ile Thr Ser Glu Val Val Ala Ser Leu Trp Ala Thr
405 410 415
Gly Gln Thr Val Leu Gly Val Ala Ser Ala Arg Phe Thr Leu Arg Asn
420 425 430
Leu Phe Asn Asn Leu Thr Gln Ala Leu Val Tyr Glu Asn Pro Ile Ser
435 440 445
Ser Ser Phe Asn Arg Ser Thr Leu Thr His Glu Leu Pro Gly Glu Asn
450 455 460
Ser Asp Arg Pro Thr Ser Ser Asp Tyr Ser His Arg Leu Ser Ser Ile
465 470 475 480
Thr Gly Phe Arg Ala Gly Ala Asn Gly Thr Val Pro Val Phe Gly Trp
485 490 495
Thr Ser Ala Thr Val Asp Arg Asn Asn Ile Ile Glu Arg Asn Lys Ile
500 505 510
Thr Gln Phe Pro Gly Val Lys Ser His Thr Leu Asn Asn Cys Gln Val
515 520 525
Val Arg Gly Thr Gly Phe Thr Gly Gly Asp Trp Leu Arg Pro Asn Asn
530 535 540
Asn Gly Thr Phe Arg Leu Thr Ile Thr Ser Phe Ser Ser Gln Ser Tyr
545 550 555 560
Arg Ile Arg Leu Arg Tyr Ala Thr Ser Val Gly Asn Thr Ser Leu Val
565 570 575
Ile Ser Ser Ser Asp Gly Gly Ile Ser Ser Thr Thr Ile Pro Leu Thr
580 585 590
Ser Thr Ile Thr Ser Leu Pro Gln Thr Val Pro Tyr Gln Ala Phe Arg
595 600 605
Val Val Asp Leu Pro Ile Thr Phe Thr Thr Pro Thr Thr Gln Arg Asn
610 615 620
Tyr Thr Phe Asp Phe Arg Leu Gln Asn Pro Ser Asn Ala Asn Val Phe
625 630 635 640
Ile Asp Arg Ile Glu Phe Val Pro Ile Gly Gly Ser Leu Ser Glu Tyr
645 650 655
Glu Thr Lys His Gln Leu Glu Lys Ala Arg Lys Ala Val Asn Asp Leu
660 665 670
Phe Thr Asn Glu Ser Lys Asn Val Leu Lys Lys Asp Thr Thr Asp Tyr
675 680 685
Asp Ile Asp Gln Ala Ala Asn Leu Val Glu Cys Val Ser Asp Glu Cys
690 695 700
Ala Asn Ala Lys Met Ile Leu Leu Asp Glu Val Lys Tyr Ala Lys Gln
705 710 715 720
Leu Ser Glu Ala Arg Asn Leu Leu Leu Asn Gly Asn Phe Asp Asn Ile
725 730 735
Asp Arg Asp Gly Glu Asn Pro Trp Lys Thr Ser Pro Asn Val Thr Ile
740 745 750
Gln Glu Asn Asn Pro Ile Phe Lys Gly Arg Tyr Leu Ser Met Ser Gly
755 760 765
Ala Asn Asn Ile Glu Ala Thr Asn Glu Ile Phe Pro Thr Tyr Ala Tyr
770 775 780
Gln Lys Ile Asp Glu Ala Lys Leu Lys Pro Tyr Thr Arg Tyr Lys Val
785 790 795 800
Arg Gly Phe Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Thr Arg
805 810 815
Tyr Asp Glu Glu Val Asp Ala Ile Leu Asn Val Pro Asn Asp Ile Pro
820 825 830
His Ala Pro Pro Pro Phe Cys Gly Glu Phe Asp Arg Cys Asn Pro His
835 840 845
Ser Tyr Pro Pro Met Asn Pro Glu Cys His His Asp Val Ile Asn Asn
850 855 860
Ile Glu Ile Ser Ser Pro Cys Gln His Asn Lys Met Val Asp Asn Ala
865 870 875 880
Asp Ile Ser Tyr Arg His Ser His Lys Lys His Gly Ile Cys His Glu
885 890 895
Ser His His Phe Glu Phe His Ile Asp Thr Gly Lys Ile Asp Leu Val
900 905 910
Glu Asn Leu Gly Ile Trp Val Ile Phe Lys Ile Cys Ser Thr Asp Gly
915 920 925
Tyr Ala Thr Leu Asp Asn Leu Glu Val Ile Glu Glu Arg Pro Leu Gly
930 935 940
Ala Glu Ser Leu Glu Arg Val Lys Arg Arg Glu Lys Lys Trp Lys His
945 950 955 960
His Met Glu His Lys Cys Ser Glu Thr Lys Leu Ala Tyr His Ala Ala
965 970 975
Lys Gln Ala Leu Val Gly Leu Phe Thr Asn Thr Glu Tyr Asp Arg Leu
980 985 990
Lys Phe Glu Thr Thr Ile Ser Asn Ile Leu Phe Ala Asp Tyr Leu Val
995 1000 1005
Gln Ser Ile Pro Tyr Val Tyr Asn Lys Trp Leu Pro Asp Val Pro Gly
1010 1015 1020
Met Asn Phe Glu Ile Tyr Thr Glu Leu Lys Asn Leu Tyr Thr Gly Ala
1025 1030 1035 1040
Phe Asn Leu Tyr Asp Gln Arg Asn Ile Ile Lys Asn Gly Asp Phe Asn
1045 1050 1055
Arg Gly Leu Met His Trp His Ala Thr Pro His Ala Arg Val Glu Gln
1060 1065 1070
Ile Asp Asn Arg Ser Val Leu Val Leu Pro Asn Tyr Ala Ala Asn Val
1075 1080 1085
Ser Gln Glu Val Cys Leu Glu His Asn Arg Gly Tyr Val Leu Arg Val
1090 1095 1100
Thr Ala Lys Lys Glu Gly Pro Gly Ile Gly Tyr Ile Thr Phe Ser Asp
1105 1110 1115 1120
Cys Ala Asn Asn Ile Glu Lys Leu Thr Phe Thr Ser Cys Asp Tyr Gly
1125 1130 1135
Thr Asn Glu Val Pro Tyr Glu Gln Ser Asn Tyr Pro Thr Asp Gly Val
1140 1145 1150
Ser Tyr Gly His His Gly Cys Asn Ile Asp Arg Val Arg Tyr Glu Glu
1155 1160 1165
Ser Gly Tyr Arg Thr Asp Gly Val Pro Tyr Glu Gln Ser Gly Tyr Arg
1170 1175 1180
Ala Asp Gly Val Ser Tyr Glu Gln His Gly Cys His Thr Asp Gly Val
1185 1190 1195 1200
Pro Tyr Lys Gln His Gly Cys Arg Thr Asp Arg Ser Arg Asp Glu Gln
1205 1210 1215
Leu Gly Tyr Val Thr Lys Thr Ile Asp Val Phe Pro Asp Thr Asp Lys
1220 1225 1230
Val Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe Lys Val Glu Ser
1235 1240 1245
Val Glu Leu Ile Cys Met Glu Glu
1250 1255
<210> 49
<211> 649
<212> PRT
<213> 侧孢短芽孢杆菌
<400> 49
Met Asn Gln Asn Gln Asn Gln Asn Gln Asn Lys Asn Glu Leu Gln Ile
1 5 10 15
Ile Glu Pro Ser Ser Asp Ser Phe Leu Tyr Ser His Asn Asn Tyr Pro
20 25 30
Tyr Ala Thr Asp Pro Asn Thr Val Leu Gln Gly Arg Asn Tyr Lys Glu
35 40 45
Trp Leu Asn Met Cys Thr Gly Thr Asp Asp Ser Arg Ser Pro Glu Ala
50 55 60
Ala Ser Thr Ala Lys Ser Ala Ile Ser Val Ala Ile Thr Ile Ser Thr
65 70 75 80
Thr Ile Leu Gly Leu Leu Gly Val Pro Phe Ala Ser Gln Ile Gly Ala
85 90 95
Phe Tyr Asn Phe Val Leu Asn Thr Val Trp Pro Gln Gly Asn Asn Gln
100 105 110
Trp Glu Glu Phe Met Arg His Val Glu Asp Leu Ile Asn Glu Arg Ile
115 120 125
Ala Asp Tyr Ala Arg Ser Lys Ala Leu Ala Glu Leu Ala Gly Leu Gly
130 135 140
Asn Asn Leu Asp Leu Tyr Arg Glu Ala Phe Glu Asp Trp Arg Arg Asn
145 150 155 160
Pro Thr Ser Gln Gln Ala Lys Thr Arg Val Ile Glu Arg Phe Arg Ile
165 170 175
Leu Asp Gly Leu Phe Glu Gln Tyr Met Pro Ser Phe Ala Val Gln Gly
180 185 190
Phe Gln Val Gln Leu Leu Thr Val Tyr Ala Ser Ala Ala Asn Ile His
195 200 205
Leu Phe Leu Leu Arg Asp Ser Ser Ile Tyr Gly Leu Asp Trp Gly Leu
210 215 220
Ser Gln Thr Asn Val Asn Glu Asn Tyr Asn Arg Gln Ile Arg His Ala
225 230 235 240
Ala Thr Tyr Ala Asn His Cys Thr Thr Trp Tyr Gln Thr Gly Leu Gln
245 250 255
Arg Leu Gln Gly Thr Asn Ala Thr Ser Trp Val Ala Tyr Asn Arg Phe
260 265 270
Arg Arg Glu Met Thr Leu Thr Val Leu Asp Ile Ser Ser Leu Phe Ser
275 280 285
Asn Tyr Asp Tyr Arg Ser Tyr Pro Thr Glu Val Arg Gly Glu Leu Thr
290 295 300
Arg Glu Ile Tyr Thr Asp Pro Val Gly Arg Asn Trp Gln Asn Ser Ala
305 310 315 320
Pro Ser Phe Ala Gln Ile Glu Asn Leu Ala Ile Arg Ala Pro Arg Thr
325 330 335
Val Thr Trp Leu Asn Ser Thr Arg Ile Ser Thr Gly Thr Leu Gln Gly
340 345 350
Trp Ser Gly Ser Asn Arg Tyr Trp Ala Ala His Met Gln Asn Phe Ser
355 360 365
Glu Thr Asn Ser Gly Asn Ile Arg Phe Asp Gly Pro Leu Tyr Gly Ser
370 375 380
Thr Val Gly Thr Ile His Arg Thr Asp Asp Tyr Asp Met Gly Asn Arg
385 390 395 400
Asp Ile Tyr Thr Ile Thr Ser Glu Val Val Ala Ser Leu Trp Ala Thr
405 410 415
Gly Gln Thr Val Leu Gly Val Ala Ser Ala Arg Phe Thr Leu Arg Asn
420 425 430
Leu Phe Asn Asn Leu Thr Gln Ala Leu Val Tyr Glu Asn Pro Ile Ser
435 440 445
Ser Ser Phe Asn Arg Ser Thr Leu Thr His Glu Leu Pro Gly Glu Asn
450 455 460
Ser Asp Arg Pro Thr Ser Ser Asp Tyr Ser His Arg Leu Ser Ser Ile
465 470 475 480
Thr Gly Phe Arg Ala Gly Ala Asn Gly Thr Val Pro Val Phe Gly Trp
485 490 495
Thr Ser Ala Thr Val Asp Arg Asn Asn Ile Ile Glu Arg Asn Lys Ile
500 505 510
Thr Gln Phe Pro Gly Val Lys Ser His Thr Leu Asn Asn Cys Gln Val
515 520 525
Val Arg Gly Thr Gly Phe Thr Gly Gly Asp Trp Leu Arg Pro Asn Asn
530 535 540
Asn Gly Thr Phe Arg Leu Thr Ile Thr Ser Phe Ser Ser Gln Ser Tyr
545 550 555 560
Arg Ile Arg Leu Arg Tyr Ala Thr Ser Val Gly Asn Thr Ser Leu Val
565 570 575
Ile Ser Ser Ser Asp Gly Gly Ile Ser Ser Thr Thr Ile Pro Leu Thr
580 585 590
Ser Thr Ile Thr Ser Leu Pro Gln Thr Val Pro Tyr Gln Ala Phe Arg
595 600 605
Val Val Asp Leu Pro Ile Thr Phe Thr Thr Pro Thr Thr Gln Arg Asn
610 615 620
Tyr Thr Phe Asp Phe Arg Leu Gln Asn Pro Ser Asn Ala Asn Val Phe
625 630 635 640
Ile Asp Arg Ile Glu Phe Val Pro Ile
645
<210> 50
<211> 317
<212> PRT
<213> 侧孢短芽孢杆菌
<400> 50
Met Lys Lys Phe Ala Ser Leu Ile Leu Ile Ser Val Phe Leu Phe Ser
1 5 10 15
Ser Thr Gln Phe Val His Ala Ser Ser Thr Asp Val Gln Glu Arg Leu
20 25 30
Arg Asp Leu Ala Arg Glu Asn Glu Ala Gly Thr Leu Asn Glu Ala Trp
35 40 45
Asn Thr Asn Phe Lys Pro Ser Asp Glu Gln Gln Phe Ser Tyr Ser Pro
50 55 60
Thr Glu Gly Ile Val Phe Leu Thr Pro Pro Lys Asn Val Ile Gly Glu
65 70 75 80
Arg Arg Ile Ser Gln Tyr Lys Val Asn Asn Ala Trp Ala Thr Leu Glu
85 90 95
Gly Ser Pro Thr Glu Val Ser Gly Thr Pro Leu Tyr Val Gly Lys Asn
100 105 110
Val Leu Asp Asn Ser Lys Gly Thr Ser Asp Gln Glu Leu Leu Thr Pro
115 120 125
Glu Phe Asn Tyr Thr Tyr Thr Glu Ser Thr Ser Asn Thr Thr Thr His
130 135 140
Gly Leu Lys Leu Gly Val Lys Thr Thr Ala Thr Met Lys Phe Pro Ile
145 150 155 160
Ala Gln Gly Ser Met Glu Ala Ser Thr Glu Tyr Asn Phe Gln Asp Ser
165 170 175
Ser Thr Asp Thr Thr Thr Lys Thr Val Ser Tyr Lys Ser Pro Ser Gln
180 185 190
Lys Ile Lys Val Pro Ala Gly Lys Thr Phe Arg Val Leu Ala Tyr Leu
195 200 205
Asn Thr Gly Ser Ile Ser Gly Glu Ala Asn Leu Tyr Ala Asn Val Gly
210 215 220
Gly Ile Ala Trp Gly Val Leu Pro Gly Tyr Pro Asn Gly Gly Gly Val
225 230 235 240
Asn Ile Gly Ala Val Leu Thr Lys Cys Gln Gln Lys Gly Trp Gly Asp
245 250 255
Phe Arg Asn Phe Gln Pro Ser Gly Arg Asp Val Ile Val Lys Gly Gln
260 265 270
Gly Thr Phe Lys Ser Asn Tyr Gly Thr Asp Phe Ile Leu Lys Ile Glu
275 280 285
Asp Ile Thr Asp Ser Lys Leu Arg Asn Asn Asn Gly Ser Gly Thr Val
290 295 300
Val Gln Glu Ile Lys Val Pro Leu Ile Arg Thr Glu Ile
305 310 315
<210> 51
<211> 1154
<212> PRT
<213> 苏云金芽孢杆菌
<400> 51
Met Asn Phe Leu Phe Leu Val Asn Tyr Glu Lys Asn Lys Phe Lys Tyr
1 5 10 15
Asn Ile Gln Gly Asp Leu Asn Met Asn Gln Lys Asn Tyr Asp Ile Ile
20 25 30
Gly Ser Ser Thr Asn Gly Thr Thr Lys Leu Pro Glu Asp Tyr Asn Ile
35 40 45
Ile Ile Ser Pro Asp Ala Ala Pro Glu Ala Val Thr Ile Ala Ile Ser
50 55 60
Ile Thr Gly Glu Val Leu Ser Leu Phe Gly Val Pro Gly Ala Thr Leu
65 70 75 80
Gly Ser Thr Leu Leu Asn Thr Leu Val Asp Lys Leu Trp Pro Thr Asn
85 90 95
Thr Asn Thr Val Trp Gly Thr Phe Thr Glu Glu Thr Ala Lys Leu Ile
100 105 110
Asn Glu Val Tyr Asn Pro Ser Asp Pro Val Val Lys Asp Ala Asp Ala
115 120 125
Arg Leu Thr Ser Leu His Glu Ser Leu Lys Leu Tyr Gln Leu Ala Phe
130 135 140
Gly Asn Trp Phe Lys Ser Gln Asp Asn Ser Lys Leu Lys Glu Glu Val
145 150 155 160
Arg Arg Gln Phe Asp Ile Thr His Asn Arg Phe Val Thr Ser Met Pro
165 170 175
Phe Phe Lys Val Ser Asp Tyr Glu Ile Arg Leu Leu Thr Asn Tyr Ala
180 185 190
Gln Ala Ala Asn Leu His Leu Thr Phe Leu Arg Asp Ala Ser Ile Tyr
195 200 205
Gly Leu Asp Trp Gly Phe Ser Asp Glu His Ser Asn Asp Leu Tyr Glu
210 215 220
Gln Gln Lys Asn Arg Thr Gly Glu Tyr Thr Asp His Cys Val Lys Trp
225 230 235 240
Tyr Asn Ala Gly Leu Glu Lys Leu Lys Gly Asn Leu Thr Gly Glu Asn
245 250 255
Trp Tyr Thr Tyr Asn Arg Phe Arg Arg Glu Met Thr Leu Met Val Leu
260 265 270
Asp Val Val Ala Leu Phe Pro Asn Tyr Asp Thr Arg Met Tyr Pro Ile
275 280 285
Ala Thr Ser Ser Glu Leu Thr Arg Met Ile Tyr Thr Asp Pro Ile Ala
290 295 300
Tyr Thr Gln Ser Asp Pro Trp Tyr Lys Ile Thr Ser Leu Ser Phe Ser
305 310 315 320
Asn Ile Glu Asn Ser Ala Ile Pro Ser Pro Ser Phe Phe Arg Trp Leu
325 330 335
Lys Ser Val Ser Ile Asn Ser Gln Trp Trp Gly Ser Gly Pro Asn Gln
340 345 350
Thr Tyr Tyr Trp Val Gly His Glu Leu Val Tyr Ser Asn Ser Asn Tyr
355 360 365
Asn Gln Ser Leu Lys Val Lys Tyr Gly Asp Pro Asn Ser Tyr Ile Glu
370 375 380
Pro Pro Asp Ser Phe Ser Phe Ser Ser Thr Asp Val Tyr Arg Thr Ile
385 390 395 400
Ser Val Val Arg Asn Ser Ile Ser Asn Tyr Ile Val Ser Glu Val Gln
405 410 415
Phe Asn Ser Ile Ser Asn Thr Asn Gln Ile Ser Glu Glu Ile Tyr Lys
420 425 430
His Gln Ser Asn Trp Asn Arg Arg Glu Thr Lys Asp Ser Ile Thr Glu
435 440 445
Leu Ser Leu Ala Ala Asn Pro Pro Thr Thr Phe Gly Asn Val Ala Glu
450 455 460
Tyr Ser His Arg Leu Ala Tyr Ile Ser Glu Ala Tyr Gln Ser Asn Asn
465 470 475 480
Pro Ser Lys Tyr Pro Ala Tyr Ile Pro Val Phe Gly Trp Thr His Thr
485 490 495
Ser Val Arg Tyr Asp Asn Lys Ile Phe Pro Asp Lys Ile Thr Gln Ile
500 505 510
Pro Ala Val Lys Ser Ser Ser Ala Glu Gly Gly Thr Trp Lys Asn Ile
515 520 525
Ala Lys Gly Pro Gly Phe Thr Gly Gly Asp Val Thr Thr Ala Val Ser
530 535 540
Pro Ala Phe Ile Thr Asp Met Ile Lys Ile His Val Thr Leu Asp Pro
545 550 555 560
Asn Ser Leu Ser Gln Lys Tyr Arg Ala Arg Leu Arg Tyr Ala Ser Asn
565 570 575
Ala Tyr Val Ala Ala Thr Leu Tyr Thr Asn Ser Ser Ser Asn Tyr Asn
580 585 590
Phe Glu Leu Thr Lys Gly Thr Thr Glu Gln Phe Thr Thr Tyr Asn Ser
595 600 605
Tyr Gln Tyr Val Asp Ile Pro Gly Ser Ile Gln Phe Asn Thr Thr Ser
610 615 620
Asp Thr Val Ser Val Tyr Leu His Met Asp Ser Thr Thr Asn Ala Asn
625 630 635 640
Val His Val Asp Arg Ile Glu Phe Ile Pro Val Asp Glu Asn Tyr Asp
645 650 655
Asn Arg Val Thr Leu Glu Lys Ala Gln Lys Ala Val Asn Ala Leu Phe
660 665 670
Thr Ala Gly Arg His Ala Leu Gln Thr Asp Val Thr Asp Phe Lys Val
675 680 685
Asp Gln Val Ser Ile Leu Val Asp Cys Val Ser Gly Glu Leu Tyr Pro
690 695 700
Asn Glu Lys Arg Glu Leu Leu Ser Leu Val Lys Tyr Ala Lys Arg Leu
705 710 715 720
Ser Tyr Ser Arg Asn Leu Leu Leu Asp Pro Thr Phe Asp Ser Ile Asn
725 730 735
Ser Ser Glu Glu Asn Gly Trp His Gly Ser Asn Gly Ile Ala Ile Gly
740 745 750
Asn Gly Asn Phe Val Phe Lys Gly Asn Tyr Leu Ile Phe Ser Gly Thr
755 760 765
Asn Asp Thr Gln Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser
770 775 780
Lys Leu Lys Glu Tyr Thr Arg Tyr Lys Leu Arg Gly Phe Ile Glu Ser
785 790 795 800
Ser Gln Asp Leu Glu Ala Tyr Val Ile Arg Tyr Asp Ala Lys Tyr Glu
805 810 815
Thr Leu Asp Val Ser Asn Asn Leu Tyr Pro Asp Ile Ser Pro Val Asn
820 825 830
Ala Cys Gly Glu Pro Asn Arg Cys Ala Ala Leu Pro Tyr Leu Asp Glu
835 840 845
Asn Pro Arg Leu Glu Cys Ser Ser Ile Gln Asp Gly Ile Leu Ser Asp
850 855 860
Ser His Ser Phe Ser Leu Asn Ile Asp Thr Gly Ser Ile Asp Ser Asn
865 870 875 880
Glu Asn Val Gly Ile Trp Val Leu Phe Lys Ile Ser Thr Pro Glu Gly
885 890 895
Tyr Ala Lys Phe Gly Asn Leu Glu Val Ile Glu Asp Gly Pro Val Ile
900 905 910
Gly Glu Ala Leu Ala Arg Val Lys Arg Gln Glu Thr Lys Trp Arg Asn
915 920 925
Lys Leu Thr Gln Leu Arg Thr Glu Thr Gln Ala Ile Tyr Thr Arg Ala
930 935 940
Lys Gln Ala Ile Asp Asn Leu Phe Thr Asn Ala Gln Asp Ser His Leu
945 950 955 960
Lys Ile Gly Ala Thr Phe Ala Ser Ile Val Ala Ala Arg Lys Ile Val
965 970 975
Gln Ser Ile Arg Glu Ala Tyr Met Pro Trp Leu Ser Ile Val Pro Gly
980 985 990
Val Asn Tyr Pro Ile Phe Thr Glu Leu Asn Glu Arg Val Gln Gln Ala
995 1000 1005
Phe Gln Leu Tyr Asp Val Arg Asn Val Val Arg Asn Gly Arg Phe Leu
1010 1015 1020
Asn Gly Val Ser Asp Trp Ile Val Thr Ser Asp Val Thr Val Gln Glu
1025 1030 1035 1040
Glu Asn Gly Asn Asn Val Leu Val Leu Ser Asn Trp Asp Ala Gln Val
1045 1050 1055
Leu Gln Cys Leu Lys Leu Tyr Gln Asp Arg Gly Tyr Ile Leu Arg Val
1060 1065 1070
Thr Ala Arg Lys Glu Gly Leu Gly Glu Gly Tyr Ile Thr Ile Thr Asp
1075 1080 1085
Glu Glu Gly Tyr Thr Asp Gln Leu Thr Phe Gly Thr Cys Glu Glu Ile
1090 1095 1100
Asp Ala Ser Asn Thr Phe Val Ser Thr Gly Tyr Ile Thr Lys Glu Leu
1105 1110 1115 1120
Glu Phe Phe Pro Asp Thr Glu Lys Val Arg Ile Glu Val Gly Glu Thr
1125 1130 1135
Glu Gly Thr Phe Arg Val Glu Ser Val Glu Leu Phe Leu Met Glu Glu
1140 1145 1150
His Cys
<210> 52
<211> 651
<212> PRT
<213> 苏云金芽孢杆菌
<400> 52
Met Asn Phe Leu Phe Leu Val Asn Tyr Glu Lys Asn Lys Phe Lys Tyr
1 5 10 15
Asn Ile Gln Gly Asp Leu Asn Met Asn Gln Lys Asn Tyr Asp Ile Ile
20 25 30
Gly Ser Ser Thr Asn Gly Thr Thr Lys Leu Pro Glu Asp Tyr Asn Ile
35 40 45
Ile Ile Ser Pro Asp Ala Ala Pro Glu Ala Val Thr Ile Ala Ile Ser
50 55 60
Ile Thr Gly Glu Val Leu Ser Leu Phe Gly Val Pro Gly Ala Thr Leu
65 70 75 80
Gly Ser Thr Leu Leu Asn Thr Leu Val Asp Lys Leu Trp Pro Thr Asn
85 90 95
Thr Asn Thr Val Trp Gly Thr Phe Thr Glu Glu Thr Ala Lys Leu Ile
100 105 110
Asn Glu Val Tyr Asn Pro Ser Asp Pro Val Val Lys Asp Ala Asp Ala
115 120 125
Arg Leu Thr Ser Leu His Glu Ser Leu Lys Leu Tyr Gln Leu Ala Phe
130 135 140
Gly Asn Trp Phe Lys Ser Gln Asp Asn Ser Lys Leu Lys Glu Glu Val
145 150 155 160
Arg Arg Gln Phe Asp Ile Thr His Asn Arg Phe Val Thr Ser Met Pro
165 170 175
Phe Phe Lys Val Ser Asp Tyr Glu Ile Arg Leu Leu Thr Asn Tyr Ala
180 185 190
Gln Ala Ala Asn Leu His Leu Thr Phe Leu Arg Asp Ala Ser Ile Tyr
195 200 205
Gly Leu Asp Trp Gly Phe Ser Asp Glu His Ser Asn Asp Leu Tyr Glu
210 215 220
Gln Gln Lys Asn Arg Thr Gly Glu Tyr Thr Asp His Cys Val Lys Trp
225 230 235 240
Tyr Asn Ala Gly Leu Glu Lys Leu Lys Gly Asn Leu Thr Gly Glu Asn
245 250 255
Trp Tyr Thr Tyr Asn Arg Phe Arg Arg Glu Met Thr Leu Met Val Leu
260 265 270
Asp Val Val Ala Leu Phe Pro Asn Tyr Asp Thr Arg Met Tyr Pro Ile
275 280 285
Ala Thr Ser Ser Glu Leu Thr Arg Met Ile Tyr Thr Asp Pro Ile Ala
290 295 300
Tyr Thr Gln Ser Asp Pro Trp Tyr Lys Ile Thr Ser Leu Ser Phe Ser
305 310 315 320
Asn Ile Glu Asn Ser Ala Ile Pro Ser Pro Ser Phe Phe Arg Trp Leu
325 330 335
Lys Ser Val Ser Ile Asn Ser Gln Trp Trp Gly Ser Gly Pro Asn Gln
340 345 350
Thr Tyr Tyr Trp Val Gly His Glu Leu Val Tyr Ser Asn Ser Asn Tyr
355 360 365
Asn Gln Ser Leu Lys Val Lys Tyr Gly Asp Pro Asn Ser Tyr Ile Glu
370 375 380
Pro Pro Asp Ser Phe Ser Phe Ser Ser Thr Asp Val Tyr Arg Thr Ile
385 390 395 400
Ser Val Val Arg Asn Ser Ile Ser Asn Tyr Ile Val Ser Glu Val Gln
405 410 415
Phe Asn Ser Ile Ser Asn Thr Asn Gln Ile Ser Glu Glu Ile Tyr Lys
420 425 430
His Gln Ser Asn Trp Asn Arg Arg Glu Thr Lys Asp Ser Ile Thr Glu
435 440 445
Leu Ser Leu Ala Ala Asn Pro Pro Thr Thr Phe Gly Asn Val Ala Glu
450 455 460
Tyr Ser His Arg Leu Ala Tyr Ile Ser Glu Ala Tyr Gln Ser Asn Asn
465 470 475 480
Pro Ser Lys Tyr Pro Ala Tyr Ile Pro Val Phe Gly Trp Thr His Thr
485 490 495
Ser Val Arg Tyr Asp Asn Lys Ile Phe Pro Asp Lys Ile Thr Gln Ile
500 505 510
Pro Ala Val Lys Ser Ser Ser Ala Glu Gly Gly Thr Trp Lys Asn Ile
515 520 525
Ala Lys Gly Pro Gly Phe Thr Gly Gly Asp Val Thr Thr Ala Val Ser
530 535 540
Pro Ala Phe Ile Thr Asp Met Ile Lys Ile His Val Thr Leu Asp Pro
545 550 555 560
Asn Ser Leu Ser Gln Lys Tyr Arg Ala Arg Leu Arg Tyr Ala Ser Asn
565 570 575
Ala Tyr Val Ala Ala Thr Leu Tyr Thr Asn Ser Ser Ser Asn Tyr Asn
580 585 590
Phe Glu Leu Thr Lys Gly Thr Thr Glu Gln Phe Thr Thr Tyr Asn Ser
595 600 605
Tyr Gln Tyr Val Asp Ile Pro Gly Ser Ile Gln Phe Asn Thr Thr Ser
610 615 620
Asp Thr Val Ser Val Tyr Leu His Met Asp Ser Thr Thr Asn Ala Asn
625 630 635 640
Val His Val Asp Arg Ile Glu Phe Ile Pro Val
645 650
<210> 53
<211> 1206
<212> PRT
<213> 苏云金芽孢杆菌
<400> 53
Met Arg Leu Lys Lys Leu Leu Val Cys Asn Ile Arg Ile Gly Gly Thr
1 5 10 15
Asn Met Asn Leu Gly Asn Tyr Asn Glu Phe Asp Ile Ile Asp Ile Thr
20 25 30
Glu Asn Asn Gln Thr Lys Thr Ser Arg Tyr Asn Asn Val Asn Arg Gln
35 40 45
Glu Asn Pro Ser Asn Met Ile Ile Ser Asn Pro Ser Ser Asn Tyr Pro
50 55 60
Leu Ala Asn Asn Pro Asn Thr Pro Phe Gln Asn Ile Asn Tyr Lys Asp
65 70 75 80
Phe Leu Asn Met Asn Glu Glu Ile Ala Pro Tyr Ala Ser Ser Lys Asp
85 90 95
Val Ile Phe Ser Ser Met Asn Ile Ile Arg Thr Phe Met Gly Phe Ala
100 105 110
Gly His Gly Thr Ala Gly Gly Ile Val Ala Leu Phe Thr Glu Val Leu
115 120 125
Arg Leu Leu Trp Pro Asn Lys Gln Asp Glu Leu Trp Glu Ser Phe Met
130 135 140
Lys Glu Val Glu Lys Leu Ile Glu Gln Glu Ile Thr Asp Ala Val Val
145 150 155 160
Ser Lys Ala Leu Ala Glu Leu Glu Gly Leu Arg Asn Ala Leu Gln Gly
165 170 175
Tyr Thr Asp Ala Leu Glu Ala Trp Gln Asn Asn Arg Ser Asp Lys Leu
180 185 190
Lys Gln Leu Leu Val Tyr Asp Arg Phe Val Ser Thr Glu Asn Leu Phe
195 200 205
Lys Phe Ala Met Pro Ser Phe Arg Val Gly Gly Phe Glu Val Pro Leu
210 215 220
Leu Thr Val Tyr Ala Gln Ala Ala Asn Leu His Leu Leu Leu Leu Lys
225 230 235 240
Asn Ser Glu Leu Phe Gly Ala Glu Trp Gly Met Gln Gln Tyr Glu Ile
245 250 255
Asp Leu Phe Tyr Asn Glu Gln Lys Asp Tyr Val Val Glu Tyr Thr Asp
260 265 270
His Cys Val Lys Trp Tyr Thr Glu Gly Leu Asn Arg Leu Lys Asn Ala
275 280 285
Ser Gly Val Lys Gly Lys Val Trp Glu Glu Tyr Asn Arg Phe Arg Arg
290 295 300
Glu Met Thr Ile Met Val Leu Asp Leu Leu Pro Leu Phe Pro Ile Tyr
305 310 315 320
Asp Val Arg Thr Tyr Pro Thr Glu Thr Val Thr Glu Leu Thr Arg Gln
325 330 335
Ile Phe Thr Asp Pro Ile Gly Leu Arg Gly Ile Asn Glu Ser Lys Tyr
340 345 350
Pro Asp Trp Tyr Gly Ala Ala Ser Asp Ser Phe Ser Leu Ile Glu Asn
355 360 365
Arg Ala Val Pro Gln Pro Ser Leu Phe Gln Trp Leu Thr Glu Phe Lys
370 375 380
Val Tyr Thr Lys Tyr Val Glu Pro Asn Asp Lys Leu Thr Ile Leu Ala
385 390 395 400
Gly His Ser Val Thr Thr Gln Tyr Thr Ser Tyr Tyr Lys Lys Ser Thr
405 410 415
Phe Thr Tyr Gly Asp Thr Ser Ser Ala Asn Ser Ser Arg Thr Phe Asp
420 425 430
Leu Leu Ala Lys Asp Val Tyr Gln Val Asp Ser Val Ala Ala Ala Ser
435 440 445
Lys Ser Ala Thr Trp Tyr Leu Ala Val Pro Glu Met Arg Leu Tyr Ser
450 455 460
Ile Asn Thr Asn Asn Ile Leu Ser Glu Asp Tyr Phe Ser Leu Ser Thr
465 470 475 480
Asn Ile Pro Ser Ser Lys Met Arg Arg Met Tyr Ser Ser Glu Glu Leu
485 490 495
Pro Ile Gly Ile Ser Asp Thr Pro Ile Tyr Gly Asp Leu Glu Glu Tyr
500 505 510
Ser His Arg Leu Ser Phe Ile Ser Glu Ile Met His Asn Ser Gly Ser
515 520 525
Val Thr Gly Ser Asn Asn Ile Lys Gly Ile Ile Pro Val Leu Gly Trp
530 535 540
Thr His Thr Ser Val Ser Pro Glu Asn Tyr Ile His Arg Asp Lys Ile
545 550 555 560
Ser Gln Leu Tyr Ala Val Lys Ala Tyr Thr Thr Ser Asn Val Ser Val
565 570 575
Val Gly Gly Pro Gly Phe Leu Gly Gly Asn Ile Ile Lys Gly Asn Asn
580 585 590
Asp Pro Ala Ser Tyr Thr Gly Ser Val Ser Trp Ala Ile Arg Leu Asp
595 600 605
Gly Ser Thr Val Ser Arg Phe Arg Leu Arg Ile Pro Tyr Ala Ala Glu
610 615 620
Thr Asp Gly Thr Phe Ser Ile Thr Val Arg Asp Asp Leu Gly Pro Phe
625 630 635 640
Thr Ile Lys Lys Asp Phe Ile Ala Thr Met Lys Pro Gly Asp Pro Leu
645 650 655
Ser Tyr Gly Lys Phe Glu Tyr Leu Glu Phe Glu Gln Thr Met Ser Leu
660 665 670
Asn Asn Lys His Gly Gln Phe Phe Val His Thr Glu Asn Leu Lys Asp
675 680 685
Arg Asn Ser Ser Val Tyr Trp Asn Arg Val Glu Ile Ile Pro Val Asp
690 695 700
Glu Asn Tyr Asp Asn Arg Val Arg Leu Glu Lys Ala Gln Lys Ala Val
705 710 715 720
Asn Ala Leu Phe Thr Ala Gly Arg His Ala Leu Gln Thr Asn Val Thr
725 730 735
Asp Tyr Lys Val Asp Gln Val Ser Ile Leu Val Asp Ser Val Ser Gly
740 745 750
Glu Leu Tyr Pro Asn Glu Lys Arg Glu Leu Gln Ser Leu Val Lys Tyr
755 760 765
Ala Lys Arg Leu Ser Tyr Ser Arg Asn Leu Leu Leu Asp Pro Thr Phe
770 775 780
Asp Ser Ile Asn Ser Ser Glu Glu Asn Gly Trp Tyr Gly Ser Asn Gly
785 790 795 800
Ile Ala Ile Gly Asn Gly Asn Phe Val Phe Lys Gly Asn Tyr Leu Asn
805 810 815
Phe Ser Gly Thr Asn Asp Thr Gln Tyr Pro Thr Tyr Leu Tyr Gln Lys
820 825 830
Ile Asp Glu Ser Lys Leu Lys Glu Tyr Thr Arg Tyr Lys Leu Arg Gly
835 840 845
Phe Ile Glu Ser Ser Gln Asp Leu Glu Ala Tyr Val Val Arg Tyr Asp
850 855 860
Ala Lys His Glu Thr Leu Asp Val Ser Asn Asn Leu Phe Pro Asp Ile
865 870 875 880
Ser Pro Val Asn Ala Cys Gly Glu Pro Asn Arg Cys Ala Ala Leu Pro
885 890 895
Tyr Leu Asp Lys Asn Pro Arg Leu Glu Cys Ser Leu Ile Gln Asp Gly
900 905 910
Ile Leu Ser Asp Ser His Ser Phe Ser Leu Asn Ile Asp Thr Gly Ser
915 920 925
Ile Asp Ser Thr Glu Asn Val Gly Ile Trp Val Leu Phe Lys Ile Ser
930 935 940
Thr Pro Glu Gly Tyr Ala Lys Phe Gly Asn Leu Glu Val Ile Glu Tyr
945 950 955 960
Gly Pro Val Ile Gly Glu Ala Leu Ala Arg Val Lys Arg Gln Glu Thr
965 970 975
Lys Trp Arg Asn Lys Leu Thr Gln Leu Arg Thr Glu Thr Gln Ala Ile
980 985 990
Tyr Thr Arg Ala Lys Gln Ala Ile Asp Asn Leu Phe Thr Asn Thr Gln
995 1000 1005
Asp Ser Tyr Leu Lys Ile Gly Ala Thr Phe Ala Ser Ile Val Ala Ala
1010 1015 1020
Arg Lys Ile Val Gln Ser Ile Arg Glu Ala Tyr Met Ser Trp Leu Ser
1025 1030 1035 1040
Ile Val Pro Gly Val Asn Tyr Pro Ile Phe Thr Glu Leu Asn Glu Arg
1045 1050 1055
Val Gln Arg Ala Phe Gln Leu Tyr Asp Val Arg Asn Val Val Arg Asn
1060 1065 1070
Gly Arg Phe Leu Ser Gly Val Ser Asp Trp Ile Val Thr Ser Asp Val
1075 1080 1085
Lys Val Gln Glu Glu Asn Gly Asn Asn Val Leu Val Leu Ser Asn Trp
1090 1095 1100
Asp Ala Gln Val Leu Gln Cys Leu Lys Leu Tyr Gln Asp Arg Gly Tyr
1105 1110 1115 1120
Ile Leu Arg Val Thr Ala Arg Lys Glu Gly Leu Gly Glu Gly Tyr Ile
1125 1130 1135
Thr Ile Thr Asp Glu Glu Gly His Thr Asp Gln Leu Thr Phe Gly Thr
1140 1145 1150
Cys Glu Glu Ile Asp Ala Ser Asn Thr Phe Val Ser Thr Gly Tyr Ile
1155 1160 1165
Thr Lys Glu Leu Glu Phe Phe Pro Asp Thr Glu Lys Val Arg Ile Glu
1170 1175 1180
Ile Gly Glu Thr Glu Gly Ile Phe Lys Val Glu Ser Val Glu Leu Phe
1185 1190 1195 1200
Leu Met Glu Asp Leu Cys
1205
<210> 54
<211> 703
<212> PRT
<213> 苏云金芽孢杆菌
<400> 54
Met Arg Leu Lys Lys Leu Leu Val Cys Asn Ile Arg Ile Gly Gly Thr
1 5 10 15
Asn Met Asn Leu Gly Asn Tyr Asn Glu Phe Asp Ile Ile Asp Ile Thr
20 25 30
Glu Asn Asn Gln Thr Lys Thr Ser Arg Tyr Asn Asn Val Asn Arg Gln
35 40 45
Glu Asn Pro Ser Asn Met Ile Ile Ser Asn Pro Ser Ser Asn Tyr Pro
50 55 60
Leu Ala Asn Asn Pro Asn Thr Pro Phe Gln Asn Ile Asn Tyr Lys Asp
65 70 75 80
Phe Leu Asn Met Asn Glu Glu Ile Ala Pro Tyr Ala Ser Ser Lys Asp
85 90 95
Val Ile Phe Ser Ser Met Asn Ile Ile Arg Thr Phe Met Gly Phe Ala
100 105 110
Gly His Gly Thr Ala Gly Gly Ile Val Ala Leu Phe Thr Glu Val Leu
115 120 125
Arg Leu Leu Trp Pro Asn Lys Gln Asp Glu Leu Trp Glu Ser Phe Met
130 135 140
Lys Glu Val Glu Lys Leu Ile Glu Gln Glu Ile Thr Asp Ala Val Val
145 150 155 160
Ser Lys Ala Leu Ala Glu Leu Glu Gly Leu Arg Asn Ala Leu Gln Gly
165 170 175
Tyr Thr Asp Ala Leu Glu Ala Trp Gln Asn Asn Arg Ser Asp Lys Leu
180 185 190
Lys Gln Leu Leu Val Tyr Asp Arg Phe Val Ser Thr Glu Asn Leu Phe
195 200 205
Lys Phe Ala Met Pro Ser Phe Arg Val Gly Gly Phe Glu Val Pro Leu
210 215 220
Leu Thr Val Tyr Ala Gln Ala Ala Asn Leu His Leu Leu Leu Leu Lys
225 230 235 240
Asn Ser Glu Leu Phe Gly Ala Glu Trp Gly Met Gln Gln Tyr Glu Ile
245 250 255
Asp Leu Phe Tyr Asn Glu Gln Lys Asp Tyr Val Val Glu Tyr Thr Asp
260 265 270
His Cys Val Lys Trp Tyr Thr Glu Gly Leu Asn Arg Leu Lys Asn Ala
275 280 285
Ser Gly Val Lys Gly Lys Val Trp Glu Glu Tyr Asn Arg Phe Arg Arg
290 295 300
Glu Met Thr Ile Met Val Leu Asp Leu Leu Pro Leu Phe Pro Ile Tyr
305 310 315 320
Asp Val Arg Thr Tyr Pro Thr Glu Thr Val Thr Glu Leu Thr Arg Gln
325 330 335
Ile Phe Thr Asp Pro Ile Gly Leu Arg Gly Ile Asn Glu Ser Lys Tyr
340 345 350
Pro Asp Trp Tyr Gly Ala Ala Ser Asp Ser Phe Ser Leu Ile Glu Asn
355 360 365
Arg Ala Val Pro Gln Pro Ser Leu Phe Gln Trp Leu Thr Glu Phe Lys
370 375 380
Val Tyr Thr Lys Tyr Val Glu Pro Asn Asp Lys Leu Thr Ile Leu Ala
385 390 395 400
Gly His Ser Val Thr Thr Gln Tyr Thr Ser Tyr Tyr Lys Lys Ser Thr
405 410 415
Phe Thr Tyr Gly Asp Thr Ser Ser Ala Asn Ser Ser Arg Thr Phe Asp
420 425 430
Leu Leu Ala Lys Asp Val Tyr Gln Val Asp Ser Val Ala Ala Ala Ser
435 440 445
Lys Ser Ala Thr Trp Tyr Leu Ala Val Pro Glu Met Arg Leu Tyr Ser
450 455 460
Ile Asn Thr Asn Asn Ile Leu Ser Glu Asp Tyr Phe Ser Leu Ser Thr
465 470 475 480
Asn Ile Pro Ser Ser Lys Met Arg Arg Met Tyr Ser Ser Glu Glu Leu
485 490 495
Pro Ile Gly Ile Ser Asp Thr Pro Ile Tyr Gly Asp Leu Glu Glu Tyr
500 505 510
Ser His Arg Leu Ser Phe Ile Ser Glu Ile Met His Asn Ser Gly Ser
515 520 525
Val Thr Gly Ser Asn Asn Ile Lys Gly Ile Ile Pro Val Leu Gly Trp
530 535 540
Thr His Thr Ser Val Ser Pro Glu Asn Tyr Ile His Arg Asp Lys Ile
545 550 555 560
Ser Gln Leu Tyr Ala Val Lys Ala Tyr Thr Thr Ser Asn Val Ser Val
565 570 575
Val Gly Gly Pro Gly Phe Leu Gly Gly Asn Ile Ile Lys Gly Asn Asn
580 585 590
Asp Pro Ala Ser Tyr Thr Gly Ser Val Ser Trp Ala Ile Arg Leu Asp
595 600 605
Gly Ser Thr Val Ser Arg Phe Arg Leu Arg Ile Pro Tyr Ala Ala Glu
610 615 620
Thr Asp Gly Thr Phe Ser Ile Thr Val Arg Asp Asp Leu Gly Pro Phe
625 630 635 640
Thr Ile Lys Lys Asp Phe Ile Ala Thr Met Lys Pro Gly Asp Pro Leu
645 650 655
Ser Tyr Gly Lys Phe Glu Tyr Leu Glu Phe Glu Gln Thr Met Ser Leu
660 665 670
Asn Asn Lys His Gly Gln Phe Phe Val His Thr Glu Asn Leu Lys Asp
675 680 685
Arg Asn Ser Ser Val Tyr Trp Asn Arg Val Glu Ile Ile Pro Val
690 695 700
<210> 55
<211> 772
<212> PRT
<213> 苏云金芽孢杆菌
<400> 55
Met Val Asn Glu Asn Met Asp Met Tyr Asn Asn Asn Gly Ser Met Asn
1 5 10 15
Gly Asn Pro Asp Met Tyr Asn Lys Asn Gly Ser Met Asn Gly Asn Thr
20 25 30
Asp Val Tyr Asn Asn Asn Gly Ser Met Asn Gly Asn Pro Asp Met Tyr
35 40 45
Asn Asn Asn Gly Ser Met Asn Gly Asn Thr Asp Val Tyr Asn Asn Asn
50 55 60
Gly Ser Met Asn Gly Asn Pro Asp Val Tyr Asn Lys Asn Gly Ser Met
65 70 75 80
Asp Gly Asn Pro Asp Met Tyr Asn Asn Asn Gly Ser Met Asn Gly Asn
85 90 95
Thr Asp Val Tyr Asn Lys Asn Gly Ser Met Asn Gly Asn Pro Asp Met
100 105 110
Tyr Asn Asn Asn Gly Ser Met Asn Gly Asn Thr Asp Val Tyr Asn Asn
115 120 125
Asn Gly Ser Met Asn Gly Asn Thr Asp Asn Gln Val Pro Ala Tyr Asn
130 135 140
Ile Leu Ser Ala Glu Asn Pro Ser Asn Ile Leu Glu Ser Asp Thr Arg
145 150 155 160
Cys Thr Leu Asn Val Lys Asn Val Gln Asp Glu Ala Ile Cys Thr Gly
165 170 175
Ser Asn Leu Thr Asn Glu Ile Gly Pro Leu Val Val Pro Ile Ala Phe
180 185 190
Thr Pro Ile Ile Leu Thr Pro Ala Leu Ile Glu Val Gly Lys Trp Leu
195 200 205
Gly Val Gln Ile Gly Lys Trp Ala Leu Ser Thr Ala Leu Lys Glu Leu
210 215 220
Lys Ser Phe Leu Phe Pro Asn Ser Asp Pro Gln Arg Glu Met Glu Lys
225 230 235 240
Leu Arg Ile Glu Leu Glu Asn Ser Phe Asn Lys Lys Leu Thr Glu Asp
245 250 255
Lys Leu Asn Phe Leu Thr Ala Ala Tyr Thr Gly Phe Asn Asn Leu Ser
260 265 270
Asn Ser Phe Ile Ser Ala Thr Glu Arg Val Lys Ala Ala Glu Ile Thr
275 280 285
Leu Ala Thr Ala Pro Ser Gln Glu Asn Gln Asp Ile Leu Asp Glu Ala
290 295 300
Arg Thr Leu Ala Arg Asp Tyr Phe Val Ser Leu His Ser Gln Met Ile
305 310 315 320
Val Trp Leu Pro Gln Phe Glu Ile Ser Gly Tyr Glu Glu Ile Ser Leu
325 330 335
Pro Leu Phe Thr Gln Met Cys Thr Leu His Leu Thr His Leu Lys Asp
340 345 350
Gly Val Leu Met Gly Gln Asn Trp Gly Leu Ser Thr Asp Asp Ile Lys
355 360 365
His Phe Lys Gly Glu Phe Tyr Arg Leu Ser Asn Asp Tyr Thr Ser Arg
370 375 380
Ala Phe Asp Ser Phe His Arg Gly Phe Asn Arg Leu Arg Thr Gln Gln
385 390 395 400
Gly Thr Ala Gly Val Ile Lys Phe Arg Thr Ala Met Asn Ala Tyr Ala
405 410 415
Phe Asp Asn Ile Tyr Lys Trp Ser Leu Leu Arg Tyr Glu Gly Ile Asn
420 425 430
Pro Arg Ile Thr Arg Ser Leu Trp His Tyr Ile Gly Tyr Asn Ser Ser
435 440 445
Leu Gly Ser Asn Asp Phe Asn Thr Leu Tyr Lys Leu Met Val Gly Ile
450 455 460
Pro His Glu Arg Phe Arg Thr Val Ala Ile Gly Tyr Arg Ala Lys Thr
465 470 475 480
Gly Glu Asp Trp Lys Val Thr Gly Ala Lys Ser Thr Phe Tyr Ser Gly
485 490 495
Gly Gly Glu Trp Val Gly Asn Val Ser Lys Ala Thr Arg Ile Pro Val
500 505 510
Tyr Thr Thr Lys Thr Asp Trp Arg Gln Phe Glu Arg Arg Ile His Gly
515 520 525
Arg Leu Gly Thr Glu Gln Tyr Thr Arg Trp His Leu Thr Ile Gln Asp
530 535 540
Thr Asn Ile Ile Gly Asn Ser Tyr Leu Thr Gly Leu Pro Phe Asp Ile
545 550 555 560
Ser Tyr Pro Asp Tyr Phe Ile Arg Thr Ile Ser Ala Lys Pro Glu Ala
565 570 575
Tyr Pro Ile Tyr Lys Ser Leu Ser Leu Gly Asp Asn Pro Gly Tyr Val
580 585 590
Val Asp Asn Pro Gly Asn Asn Leu Ile Ile Gly Phe Ser Pro Asp Asn
595 600 605
Leu Lys Thr Phe Met Thr Asp Gly Asn Arg Tyr His Ser Ile Glu Ser
610 615 620
Gly Tyr Pro Thr Asn Pro Ser Cys Thr Ile Pro Ala Val Leu Tyr Asn
625 630 635 640
Ser Val Ser Asn Pro Phe Gln Ala Tyr Phe Asn Asp Glu Leu Gly Asn
645 650 655
Gly Ser Asp Gly Ser Ile Thr Leu Ile Arg Arg Gly Gly Ala His Tyr
660 665 670
Leu Val Asp Ser Arg Ser Ala Ser Tyr Asp Arg Ser Phe Arg Leu Ile
675 680 685
Ile Arg Ile Gln Ala Gly Ser Ser Ala Phe Lys Val Thr Val Arg Ser
690 695 700
Arg His Thr Ser Glu Ser Phe Glu Leu Asn Phe Thr Leu Leu Ser Asp
705 710 715 720
Gln Asp Ile Asn Tyr Tyr Tyr Asp Tyr Ile Ser Gln Pro Phe Asn Leu
725 730 735
Ser Ser Thr Tyr Tyr Tyr Ile Asp Val Glu Arg Val Val Ser Asp Asp
740 745 750
Ile Arg Ala Leu Thr Phe Asn Gln Met Ile Ile Val Pro Thr Thr Glu
755 760 765
Phe Gln Ile Leu
770
<210> 56
<211> 289
<212> PRT
<213> 苏云金芽孢杆菌
<400> 56
Met Ala Ile Tyr Asp Ile Ala Ala Asp Leu Phe Asp Leu Thr Arg Trp
1 5 10 15
Tyr Ala Glu Gln Asn Tyr Asn Ala Asn Pro Thr Thr Phe Arg Gly Ala
20 25 30
Lys Val Tyr Asp Arg Ile Val Ser Asp Val Gln Ser Ile Pro Glu Lys
35 40 45
Val Asp Phe Asn Leu Ile Pro Gly Leu Ala Tyr Thr Val Lys Asn Glu
50 55 60
Ile Val Asn Asp Thr Asn Thr Glu Gln Ser Met Ser Thr Lys Leu Met
65 70 75 80
His Thr Leu Ile Glu Ser Asn Ser Val Thr Thr Thr Lys Gly Tyr Lys
85 90 95
Ile Gly Ser Ser Ile Lys Asn Thr Phe Ser Val Asn Ile Glu Gly Ser
100 105 110
Phe Phe Val Gly Gly Gly Ser Thr Glu His Ser Ile Glu Val Ser Val
115 120 125
Ser Gly Glu Tyr Asn His Ser Ser Ser Glu Thr Lys Thr Asn Thr Ser
130 135 140
Gln Lys Thr Trp Glu Tyr Asn Ser Pro Ile Leu Val Pro Ala Lys Thr
145 150 155 160
Lys Val Thr Ala Thr Leu Asp Ile Tyr Ala Gly Pro Val Val Val Pro
165 170 175
Val Thr Leu Lys Ser Thr Val Thr Gly Thr Gly Ile Val Asn Asn Phe
180 185 190
Pro Asn Val Leu Thr Ser Leu Ser Tyr Ile Asp Arg Asn Asn Lys Leu
195 200 205
Trp Thr Asp Ser Leu Pro Thr Ala Leu Leu Tyr Asp Tyr Arg Asn Gln
210 215 220
Trp Pro Gly Ser Gln Ser Ile Tyr Val Gly Lys Asn Gly Gly Gly Val
225 230 235 240
Gln Val Glu Gly Lys Ala Glu Ile Gln Leu Glu Leu Gly Leu Tyr Ser
245 250 255
Ile Ala Thr Phe Asp Ser Gln Pro Leu Ser Gly Asn Thr Thr Gly Lys
260 265 270
Glu Ala Val Tyr Ser Lys Ala Ile Leu Arg Asp Gly Ser Ile Ile Asp
275 280 285
Ile
<210> 57
<211> 295
<212> PRT
<213> 苏云金芽孢杆菌
<400> 57
Met Ala Ile His Asp Val Gly Thr Asp Leu Leu Glu Phe Ala Lys Trp
1 5 10 15
Tyr Ala Thr Thr Asn Tyr Asn Ala Asn Pro Asn Thr Phe Arg Asn Pro
20 25 30
Gln Ile Phe Glu Ser Val Val Gly Glu Ser Glu Ile Ile Pro Lys Asn
35 40 45
Asp Thr Phe Glu Thr Ile Pro Lys Leu Thr Thr Val Val Thr Asp Val
50 55 60
Ile Ile Asn Asp Thr Ser Val Pro Gln Ser Ile Thr Pro Lys Ile Met
65 70 75 80
Gln Lys Thr Ser Glu Thr Ile Thr Thr Thr Thr Thr Gln Gly Phe Lys
85 90 95
Val Gly Ser Glu Ile Lys Tyr Thr Asn Thr Met Lys Val Asn Leu Leu
100 105 110
Leu Val Gly Gly Val Ser Asn Ser Ile Ala Val Ser Ile Ser Ala Glu
115 120 125
Tyr Asn Tyr Ser Ser Ser Glu Thr Glu Thr Asn Ile Thr Glu Lys Ala
130 135 140
Trp Glu Tyr Asn Arg Pro Val Leu Val Leu Pro Arg Thr Lys Val Thr
145 150 155 160
Ala Thr Leu Ser Ile Tyr Ser Gly Ser Phe Thr Ile Pro Val Thr Leu
165 170 175
Lys Ser Thr Ile Ser Gly Asn His Ile Ser Asn Ser Gly Tyr Gly Tyr
180 185 190
Ala Leu Ser Ser Ile Gly Tyr Thr Asp Tyr Asn Asn Arg Ser Trp Thr
195 200 205
Asp Ile Tyr Arg Thr Asn Phe Leu Tyr Asp Tyr Arg Asn Glu Trp Pro
210 215 220
Gly Arg Lys Pro Ile Tyr Val Gly Arg Asp Asn Ile Gly Val Lys Val
225 230 235 240
Glu Gly Glu Ser Arg Val Asp Ala Glu Leu Gly Leu Tyr Ser Ile Val
245 250 255
Thr Phe Lys Glu Glu Pro Leu Pro Gly Asn Asn Leu Ile Gly Asn Gly
260 265 270
Arg Thr Tyr Ser Met Ala Ile Leu Arg Asp Gly Ser Thr Met Asp Ile
275 280 285
Ser Ile Pro Lys Asn Asn Asn
290 295
<210> 58
<211> 1333
<212> PRT
<213> 苏云金芽孢杆菌
<400> 58
Met Ala Gln Leu Asn Glu Ile Tyr Pro Ser Tyr Tyr Asn Val Leu Ala
1 5 10 15
Tyr Pro Pro Leu Ile Leu Asp Asp Lys Ser Leu Tyr Asp Gln Tyr Thr
20 25 30
Glu Trp Lys Lys Lys Ile Asp Lys Thr Trp Lys Gln Tyr Asp Lys Asp
35 40 45
Phe Leu Pro Lys Pro Leu Met Asp Leu Gly Lys Ser Leu Ala Glu Ala
50 55 60
Tyr Lys Gly Asp Pro Asp Gly Tyr Leu His Ile Ala Asn Thr Ala Ile
65 70 75 80
Arg Ile Ala Phe Leu Leu Ile Pro Gly Gly Gln Thr Ala Ala Phe Gly
85 90 95
Val Asn Leu Val Leu Asn Lys Ala Ile Gly Ile Phe Tyr Pro Pro Gln
100 105 110
Asn Lys Ser Leu Phe Asp Gln Ile Lys Asp Ala Val Ser Asn Leu Val
115 120 125
Asp Gln Lys Leu Ile Asp Gln Glu Ile Ser Gly Val Leu Ile Lys Leu
130 135 140
Asn Ser Leu Gln Gln Pro Leu Ser Arg Phe Ser Asn Ser Ile Gln Arg
145 150 155 160
Ala Val Gly Lys Pro Gln Asp Phe Asp Asp Gln Thr Thr Ser Ser Asn
165 170 175
Ala Ile Ile Leu Asp Glu Thr Gln Asp Cys Ser Lys Asp Asp Ser Cys
180 185 190
Ser Cys Ser Asn Thr Gln Pro Arg Pro Ser Asp Ala Pro Leu Cys Thr
195 200 205
Pro Cys Ile Cys Arg Met Lys Glu Val Gln Gln Thr Phe Asn Asn Ser
210 215 220
Ser Thr Asp Val Asn Arg Ala Leu Thr Asp Met Lys Thr Thr Leu Lys
225 230 235 240
Asp Val Val Gly Ala Asp Gln Leu Arg Ser Tyr Met Gln Ile Tyr Leu
245 250 255
Pro Leu Tyr Val Thr Ala Ala Thr Met Glu Leu Gln Met Tyr Lys Thr
260 265 270
Tyr Ile Asp Phe Thr Gln Lys Phe Asp Phe Asp Val Thr Gly Thr Thr
275 280 285
Lys Glu His Val Asn Glu Leu Arg Gln Lys Ile Lys Thr His Ser Glu
290 295 300
Tyr Ile Met Gly Leu Phe Lys Lys Ser Leu Pro Glu Ile Ser Asn Asn
305 310 315 320
Thr Lys Glu Gln Leu Asn Ala Tyr Ile Lys Tyr Thr Arg Asn Ile Thr
325 330 335
Leu Asn Ala Leu Asp Met Val Ser Thr Trp Lys Phe Leu Asp Pro Val
340 345 350
Asp Tyr Pro Thr Thr Ala Thr Phe Asn Pro Thr Arg Ile Ile Phe Asn
355 360 365
Asp Leu Ala Gly Pro Val Glu Cys Leu Asn Ser Thr Gln Asp Ser Asn
370 375 380
Lys Leu His Phe Asn Phe Phe Asp Met Asn Gly Gln Ser Met Pro Asn
385 390 395 400
Asn Asp Ile Phe Asn Tyr Phe Tyr Arg Gly Met Gln Val Lys Gly Leu
405 410 415
Gln Ile Gln Thr Tyr Thr Ser Ser Asp Thr Lys Asn Pro Gln His Phe
420 425 430
Pro Val Gly Phe Leu Ser Ser Tyr Tyr Gly Ser Asn Gly Asp Phe Pro
435 440 445
Phe Asp Lys Arg Val Asp Pro Asn Lys Phe Thr Gly Gly Ser Lys Ser
450 455 460
Val Lys Leu Gly Asp Asp Val Tyr Glu Ser Arg Ser Ala Leu Ser Val
465 470 475 480
Ile Asn Ala Val Ser Asn Gln Leu Gln Val Phe Leu Asn Tyr Ile Asp
485 490 495
Thr Glu Asp Leu Tyr Phe Asp Gln Ser Val Ser Pro Gly Gly Thr Ala
500 505 510
Cys Gly Ser Gly Asn Ser Thr Ile Trp Pro Asp Gln Lys Ile Gln Ala
515 520 525
Ile Tyr Pro Ile Gln Pro Asp Asn Ser Gln Thr Tyr Pro Ser Tyr Tyr
530 535 540
Ser Thr Ser Lys Ile Gly Phe Val Thr Thr Leu Val Pro Asn Asp Thr
545 550 555 560
Thr Pro Trp Ile Thr Phe Thr Asp Asn Gly Asn Asn Ser Ile Tyr Thr
565 570 575
Phe Ser Ala Glu Asn Thr Arg Thr Leu Thr Gly Ser Ala Gly Pro Val
580 585 590
Arg Glu Phe Ile Thr Gly Ser Ala Pro Leu Gly Leu Ser Pro Gly Gly
595 600 605
Gly Ala Gln Tyr Ser Ile Asn Thr Ser Asp Ala Pro Ser Gly Asp Tyr
610 615 620
Gln Val Arg Val His Val Ala Thr Pro Gly Ser Gly Gly Ser Leu Ala
625 630 635 640
Ile Ser Val Asp Gly Lys Thr Gln Thr Leu Gln Leu Pro Asp Thr Asn
645 650 655
Val Asn Asp Thr Asn His Ile Ala Gly Phe Ala Gly Thr Tyr Thr Leu
660 665 670
Ala Pro Ala Thr Gln Val Asp Ala Ala Thr Leu Lys Pro Lys Ala Pro
675 680 685
Thr Glu Asn Ile Phe Pro Val Arg Gln Thr Ser Ser Leu Pro Val Ser
690 695 700
Ile Thr Asn Asn Ser Ser Thr Val Ile Asn Ile Asp Arg Ile Glu Phe
705 710 715 720
Val Pro Val Ser Ala Pro Ala Pro Asp Pro Ser Pro Asp Ser Gly Lys
725 730 735
Pro Ile His Lys Ser Val Pro Lys Thr Val Thr Gln Leu Ser Thr Thr
740 745 750
Lys Glu Ile Trp Ser Ser Thr Ser Glu Tyr Ala Thr Thr Ile Ser Phe
755 760 765
Thr Gly Asn Val Tyr Asn Asp Ala Ser Ile Thr Phe Gln Leu Leu Ser
770 775 780
Ser Gly Gln Val Val Lys Glu Phe Pro Phe Thr Gly Asn Gly Val Ala
785 790 795 800
Ser Lys Pro Gly Phe His Gly Ser Ser Pro Ser Cys Tyr Asp Thr Pro
805 810 815
Tyr Pro Phe Ser Gln Pro Asp Leu Ser Val Pro Lys Tyr Asn Lys Leu
820 825 830
Gln Val Val Met Lys Ser Asp Gly Tyr Ser Lys Pro Cys Asp Leu Gly
835 840 845
Asp Ser Phe Pro Asn Thr Phe Asp Ala Glu Ile Asp Ile Lys Phe Asn
850 855 860
Leu Ser Asp Thr Ala Asp Leu Ala Gln Ile Thr Ala Gln Val Gln Gly
865 870 875 880
Leu Phe Thr Ser Ser Ser Ser Thr Glu Leu Ser Pro Asn Val Ser Gly
885 890 895
Tyr Gln Ile Asp Gln Ile Ala Leu Lys Val Asn Ala Leu Ser Asp Glu
900 905 910
Val Phe Cys Lys Glu Lys Ile Val Leu Arg Lys Leu Val Asn Lys Ala
915 920 925
Lys Gln Phe Met Lys Thr Arg Asn Leu Leu Ile Gly Gly Asp Phe Glu
930 935 940
Ile Leu Asp Lys Trp Ala Leu Gly Thr Gln Ala Thr Ile Lys Asp Asn
945 950 955 960
Ser Ser Leu Phe Lys Gly Asn His Leu Phe Leu Gln Pro Thr Asn Gly
965 970 975
Ile Ser Ser Ser Tyr Ala Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys
980 985 990
Pro Tyr Thr Arg Tyr Asn Val Ser Gly Phe Val Ala Gln Ser Glu His
995 1000 1005
Leu Glu Ile Val Val Ser Arg Tyr Gly Lys Glu Ile Asp Lys Ile Leu
1010 1015 1020
Asn Val Pro Tyr Glu Glu Ala Leu Pro Val Ser Ser Gly Asn Gln Ser
1025 1030 1035 1040
Thr Cys Cys Lys Pro Ser Ser Cys Ser Cys Ser Ala Cys Thr Gly Gly
1045 1050 1055
Pro His Pro His Phe Phe Ser Tyr Ser Ile Asp Val Gly Lys Leu Tyr
1060 1065 1070
Pro Asp Leu Asn Pro Gly Ile Glu Phe Gly Leu Arg Leu Ala His Pro
1075 1080 1085
Ser Gly Tyr Ala Lys Val Gly Asn Leu Glu Ile Val Glu Glu Arg Pro
1090 1095 1100
Leu Thr Asn Thr Glu Ile Arg Lys Ile Gln Arg Lys Glu Glu Lys Trp
1105 1110 1115 1120
Lys Lys Ala Trp Asp Thr Glu Arg Ala Glu Ile Asn Ala Ile Leu Gln
1125 1130 1135
Pro Val Ile Asn Gln Ile Asn Ala Phe Tyr Thr Asn Gly Asp Trp Asn
1140 1145 1150
Gly Ser Ile Leu Pro His Val Thr Tyr Gln Asp Leu Tyr Asn Ile Val
1155 1160 1165
Leu Pro Glu Leu Ser Lys Leu Arg His Trp Phe Met Lys Asp Arg Pro
1170 1175 1180
Gly Glu His Tyr Thr Ile Leu Gln Gln Phe Lys Gln Ala Leu Glu Arg
1185 1190 1195 1200
Val Phe Asn Gln Leu Glu Glu Arg Asn Leu Ile His Asn Gly Ser Phe
1205 1210 1215
Thr Asn Gly Leu Ala Asn Trp Leu Val Asp Gly Asp Thr Gln Ile Thr
1220 1225 1230
Thr Leu Glu Asn Gly Asn Leu Ala Leu Gln Leu Ser Asp Trp Asp Ala
1235 1240 1245
Ser Ala Ser Gln Ser Ile Asp Ile Ser Asp Phe Asp Glu Asp Lys Glu
1250 1255 1260
Tyr Thr Val Arg Val Tyr Ala Lys Gly Lys Gly Thr Ile Arg Thr Val
1265 1270 1275 1280
Asn Cys Glu Asn Glu Pro Leu Ser Phe Asn Thr Asn Thr Phe Thr Ile
1285 1290 1295
Leu Glu Gln Arg Leu Tyr Phe Asp Asn Pro Ser Val Leu Leu His Ile
1300 1305 1310
Gln Ser Glu Gly Ser Glu Phe Val Ile Gly Ser Val Glu Leu Ile Glu
1315 1320 1325
Leu Ser Asp Asp Glu
1330
<210> 59
<211> 723
<212> PRT
<213> 苏云金芽孢杆菌
<400> 59
Met Ala Gln Leu Asn Glu Ile Tyr Pro Ser Tyr Tyr Asn Val Leu Ala
1 5 10 15
Tyr Pro Pro Leu Ile Leu Asp Asp Lys Ser Leu Tyr Asp Gln Tyr Thr
20 25 30
Glu Trp Lys Lys Lys Ile Asp Lys Thr Trp Lys Gln Tyr Asp Lys Asp
35 40 45
Phe Leu Pro Lys Pro Leu Met Asp Leu Gly Lys Ser Leu Ala Glu Ala
50 55 60
Tyr Lys Gly Asp Pro Asp Gly Tyr Leu His Ile Ala Asn Thr Ala Ile
65 70 75 80
Arg Ile Ala Phe Leu Leu Ile Pro Gly Gly Gln Thr Ala Ala Phe Gly
85 90 95
Val Asn Leu Val Leu Asn Lys Ala Ile Gly Ile Phe Tyr Pro Pro Gln
100 105 110
Asn Lys Ser Leu Phe Asp Gln Ile Lys Asp Ala Val Ser Asn Leu Val
115 120 125
Asp Gln Lys Leu Ile Asp Gln Glu Ile Ser Gly Val Leu Ile Lys Leu
130 135 140
Asn Ser Leu Gln Gln Pro Leu Ser Arg Phe Ser Asn Ser Ile Gln Arg
145 150 155 160
Ala Val Gly Lys Pro Gln Asp Phe Asp Asp Gln Thr Thr Ser Ser Asn
165 170 175
Ala Ile Ile Leu Asp Glu Thr Gln Asp Cys Ser Lys Asp Asp Ser Cys
180 185 190
Ser Cys Ser Asn Thr Gln Pro Arg Pro Ser Asp Ala Pro Leu Cys Thr
195 200 205
Pro Cys Ile Cys Arg Met Lys Glu Val Gln Gln Thr Phe Asn Asn Ser
210 215 220
Ser Thr Asp Val Asn Arg Ala Leu Thr Asp Met Lys Thr Thr Leu Lys
225 230 235 240
Asp Val Val Gly Ala Asp Gln Leu Arg Ser Tyr Met Gln Ile Tyr Leu
245 250 255
Pro Leu Tyr Val Thr Ala Ala Thr Met Glu Leu Gln Met Tyr Lys Thr
260 265 270
Tyr Ile Asp Phe Thr Gln Lys Phe Asp Phe Asp Val Thr Gly Thr Thr
275 280 285
Lys Glu His Val Asn Glu Leu Arg Gln Lys Ile Lys Thr His Ser Glu
290 295 300
Tyr Ile Met Gly Leu Phe Lys Lys Ser Leu Pro Glu Ile Ser Asn Asn
305 310 315 320
Thr Lys Glu Gln Leu Asn Ala Tyr Ile Lys Tyr Thr Arg Asn Ile Thr
325 330 335
Leu Asn Ala Leu Asp Met Val Ser Thr Trp Lys Phe Leu Asp Pro Val
340 345 350
Asp Tyr Pro Thr Thr Ala Thr Phe Asn Pro Thr Arg Ile Ile Phe Asn
355 360 365
Asp Leu Ala Gly Pro Val Glu Cys Leu Asn Ser Thr Gln Asp Ser Asn
370 375 380
Lys Leu His Phe Asn Phe Phe Asp Met Asn Gly Gln Ser Met Pro Asn
385 390 395 400
Asn Asp Ile Phe Asn Tyr Phe Tyr Arg Gly Met Gln Val Lys Gly Leu
405 410 415
Gln Ile Gln Thr Tyr Thr Ser Ser Asp Thr Lys Asn Pro Gln His Phe
420 425 430
Pro Val Gly Phe Leu Ser Ser Tyr Tyr Gly Ser Asn Gly Asp Phe Pro
435 440 445
Phe Asp Lys Arg Val Asp Pro Asn Lys Phe Thr Gly Gly Ser Lys Ser
450 455 460
Val Lys Leu Gly Asp Asp Val Tyr Glu Ser Arg Ser Ala Leu Ser Val
465 470 475 480
Ile Asn Ala Val Ser Asn Gln Leu Gln Val Phe Leu Asn Tyr Ile Asp
485 490 495
Thr Glu Asp Leu Tyr Phe Asp Gln Ser Val Ser Pro Gly Gly Thr Ala
500 505 510
Cys Gly Ser Gly Asn Ser Thr Ile Trp Pro Asp Gln Lys Ile Gln Ala
515 520 525
Ile Tyr Pro Ile Gln Pro Asp Asn Ser Gln Thr Tyr Pro Ser Tyr Tyr
530 535 540
Ser Thr Ser Lys Ile Gly Phe Val Thr Thr Leu Val Pro Asn Asp Thr
545 550 555 560
Thr Pro Trp Ile Thr Phe Thr Asp Asn Gly Asn Asn Ser Ile Tyr Thr
565 570 575
Phe Ser Ala Glu Asn Thr Arg Thr Leu Thr Gly Ser Ala Gly Pro Val
580 585 590
Arg Glu Phe Ile Thr Gly Ser Ala Pro Leu Gly Leu Ser Pro Gly Gly
595 600 605
Gly Ala Gln Tyr Ser Ile Asn Thr Ser Asp Ala Pro Ser Gly Asp Tyr
610 615 620
Gln Val Arg Val His Val Ala Thr Pro Gly Ser Gly Gly Ser Leu Ala
625 630 635 640
Ile Ser Val Asp Gly Lys Thr Gln Thr Leu Gln Leu Pro Asp Thr Asn
645 650 655
Val Asn Asp Thr Asn His Ile Ala Gly Phe Ala Gly Thr Tyr Thr Leu
660 665 670
Ala Pro Ala Thr Gln Val Asp Ala Ala Thr Leu Lys Pro Lys Ala Pro
675 680 685
Thr Glu Asn Ile Phe Pro Val Arg Gln Thr Ser Ser Leu Pro Val Ser
690 695 700
Ile Thr Asn Asn Ser Ser Thr Val Ile Asn Ile Asp Arg Ile Glu Phe
705 710 715 720
Val Pro Val
<210> 60
<211> 378
<212> PRT
<213> 苏云金芽孢杆菌
<400> 60
Met Tyr Cys Asn Thr Ile Leu Arg Lys Arg Tyr Lys Lys Leu Ala Thr
1 5 10 15
Ile Ile Pro Leu Thr Ser Met Ser Ala Val Ala Ile Ala Pro Ala Thr
20 25 30
Ser Phe Ala Val Glu Thr Gln Lys Ala Asp Val Ser Ser Gln Glu Gly
35 40 45
Pro Ile Gln Gly Tyr Gln Met Glu Asn Gly Lys Ile Thr Pro Val Tyr
50 55 60
Lys Asn Lys Leu Thr Gln Phe Asn Thr Ala Asp Asp Ile Asp Pro Gly
65 70 75 80
Leu Pro Leu Leu Pro Glu Asn Pro Tyr Asn Pro Ile Pro Asp His Gly
85 90 95
Thr Ala Tyr Val Glu Ser Thr Asp Ile Gly Asp Thr Val Tyr Phe Lys
100 105 110
Pro Phe Glu Pro Pro Lys Asn Asn Val Leu Glu Leu Gly Asp Cys Asp
115 120 125
Asp Asn Thr Tyr Gln Trp Ser Val Phe Val Asp Ser Gln Lys Tyr Lys
130 135 140
Ser Val Gly Tyr Phe Val Gln Lys Gln Ala Asp Gly Gln Ile Arg Val
145 150 155 160
Gly Tyr Tyr Asn Pro Glu Asp Leu Ser Leu Ile Thr Asp Ser Asn His
165 170 175
Ala Phe Ala Gly Val Pro Gly Phe Lys Leu Thr Ala Glu Glu Lys Ala
180 185 190
Glu Met Gln Arg Asp Leu Asn Arg Glu Tyr Gly Asp Ile Trp Asp Gly
195 200 205
Thr Ser Lys Leu Lys Arg Glu Thr Asn Tyr Lys Leu Leu Pro Asn Ala
210 215 220
Ser Gly Leu Gln Asp Asp Ala Ser Gly Phe Gly Tyr Asn Gln Thr Leu
225 230 235 240
Thr Ser Gly Val Ser Thr Thr Asn Met Phe Gly Ile Ala Thr Thr Val
245 250 255
Gly Trp Lys Met Gly Ile Lys Val Ser Val Val Pro Leu Val Ala Asp
260 265 270
Val Thr Ser Glu Ile Ser Ala Ser Leu Thr Ala Ser Tyr Gln His Thr
275 280 285
Val Asn Val Thr Asn Gln Thr Ser Ser Gln Val Lys Phe Asp Val Ser
290 295 300
Arg Val Asp Asn Pro Asp Tyr Lys Tyr Asn Asp Tyr Ala Ala Ala Val
305 310 315 320
Tyr Lys Ile Tyr Thr Asp Tyr Thr Leu Glu Pro Gly Lys Gly Leu Ser
325 330 335
Arg Phe Leu Ala Lys Gln Asp Leu Lys Asp Pro Val Arg Thr Ala Ala
340 345 350
Leu Ala Asn Thr Asn Tyr Ala Tyr Glu Gly Ser Lys Tyr Tyr Phe Thr
355 360 365
Val Thr Pro Gly Ser His Lys Lys Ile Val
370 375
<210> 61
<211> 383
<212> PRT
<213> 苏云金芽孢杆菌
<400> 61
Met Asp Phe Leu Asn Tyr Tyr Asn Lys Leu Lys Asn Glu Leu Asp Asp
1 5 10 15
Val Asn Ser Lys Lys Tyr Ser Leu Glu Tyr Thr Ser Asp Gly Leu Met
20 25 30
Val Gln Pro Thr Asp Asp Pro Leu Asn Thr Met Pro Leu Pro Asp Arg
35 40 45
Pro Val Leu Ser Gly Asn Pro Asn Asp Pro Ile Pro Ser Glu Gly Thr
50 55 60
Thr Arg Thr Asp Ile Gln Lys Gln Asn Pro Pro Phe Phe Thr Phe Lys
65 70 75 80
Val Val Ala Lys Leu Ala Tyr Ser Gly Lys Gly Glu Asn Cys Gln Lys
85 90 95
Ala Arg Ala Ala Ser Val Tyr Gly Ala Val Leu Glu Leu Glu Lys Val
100 105 110
Lys Gln Leu Pro Glu Tyr Ser Asn Val Tyr Leu Tyr Ser Glu Thr Gly
115 120 125
Ile Lys Thr Asp Arg Ser Asn Ile Arg Tyr Asn Thr Asp Gly Ile Ile
130 135 140
Gln Phe Leu Asn Pro Ser Phe Ile Asn Thr Phe Ser Ser Asn Pro Ile
145 150 155 160
Lys Tyr Gly Asp Thr Val Gly Tyr Ile Ser Tyr Pro Tyr Asp Thr Leu
165 170 175
Lys Phe Pro Ser Thr Thr Gln Leu Glu Arg Leu Val Tyr Phe Asn Leu
180 185 190
Leu Asp Ser Asn Ile Leu Asp Lys His Ile Gly Phe Asp Trp Ser Lys
195 200 205
Ser Val Thr Asn Gly Thr Glu Asp Thr Glu Met Trp Thr His Ser Ser
210 215 220
Thr Val Gly Ala Glu Leu Asn Leu Lys Asp Ile Leu Gln Ile Asn Ala
225 230 235 240
Ser Tyr Glu His Thr Phe Ser Thr Ser His Met Glu Lys Lys Glu Asn
245 250 255
Thr Val Ser Lys Thr Ala His Phe Asn Ser Pro Leu Pro Pro Tyr Asn
260 265 270
Tyr Ala Thr Trp Val Ala Ala Ile Tyr Gln Leu Ser Ile Arg Tyr Gln
275 280 285
Arg Thr Asn Ala Gln Pro Ile Leu Asp Thr Ile Asn Ala Val Asn Ser
290 295 300
Gly Leu Thr Ala Ser Glu Thr Asp Ile Tyr Leu Lys Ala Leu Tyr Gly
305 310 315 320
Ala Gly Lys Asn Gly Lys Pro Ala Val Gly Asp Pro Ser Ile Leu His
325 330 335
Lys Leu Ser Asn Val Ile Glu Asp Ala Tyr Glu Tyr Leu Tyr Tyr Ser
340 345 350
Asp Thr Leu Tyr Phe Thr Gln Thr Pro Ser Gly Asn Ser Pro Thr Pro
355 360 365
Asn Ser Pro Asn Arg Ile Gln Phe Ile Ala Thr Asp Pro Gln Ser
370 375 380
<210> 62
<211> 647
<212> PRT
<213> 人工序列
<220>
<223> 截短的变体Axmi207
<400> 62
Met Pro Ser Ser Asp Ser Phe Leu Tyr Ser His Asn Asn Tyr Pro Tyr
1 5 10 15
Ala Thr Asp Pro Asn Thr Val Leu Glu Gly Arg Asn Tyr Lys Glu Trp
20 25 30
Leu Asn Lys Cys Thr Asp Asn Tyr Thr Asp Ala Leu Gln Ser Pro Glu
35 40 45
Ala Thr Ala Ile Ser Lys Gly Ala Val Ser Ala Ala Ile Ser Ile Ser
50 55 60
Thr Lys Val Leu Gly Leu Leu Gly Val Pro Phe Ala Ala Gln Ile Gly
65 70 75 80
Gln Leu Trp Thr Phe Ile Leu Asn Ala Leu Trp Pro Ser Asp Asn Thr
85 90 95
Gln Trp Glu Glu Phe Met Arg His Val Glu Glu Leu Ile Asn Gln Arg
100 105 110
Ile Ala Asp Tyr Ala Arg Asn Lys Ala Leu Ala Glu Leu Thr Gly Leu
115 120 125
Gly Asn Asn Leu Asp Leu Tyr Ile Glu Ala Leu Asp Asp Trp Lys Arg
130 135 140
Asn Pro Thr Ser Gln Glu Ala Lys Thr Arg Val Ile Asp Arg Phe Arg
145 150 155 160
Ile Val Asp Gly Leu Phe Glu Ala Tyr Ile Pro Ser Phe Ala Val Ser
165 170 175
Gly Tyr Gln Val Gln Leu Leu Thr Val Tyr Ala Ala Ala Ala Asn Leu
180 185 190
His Leu Leu Leu Leu Arg Asp Ser Thr Ile Tyr Gly Ile Asp Trp Gly
195 200 205
Leu Ser Gln Thr Asn Val Asn Asp Asn Tyr Asn Arg Gln Ile Arg Leu
210 215 220
Thr Ala Thr Tyr Ala Asn His Cys Thr Thr Trp Tyr Gln Thr Gly Leu
225 230 235 240
Glu Arg Leu Arg Gly Ser Asn Ala Ser Ser Trp Val Thr Tyr Asn Arg
245 250 255
Phe Arg Arg Glu Met Thr Leu Thr Val Leu Asp Ile Cys Ser Leu Phe
260 265 270
Ser Asn Tyr Asp Tyr Arg Ser Tyr Pro Ala Glu Val Arg Gly Glu Ile
275 280 285
Thr Arg Glu Ile Tyr Thr Asp Pro Val Gly Val Gly Trp Val Asp Ser
290 295 300
Ala Pro Ser Phe Gly Glu Ile Glu Asn Leu Ala Ile Arg Ala Pro Arg
305 310 315 320
Thr Val Thr Trp Leu Asn Ser Thr Arg Ile Phe Thr Gly Arg Leu Gln
325 330 335
Gly Trp Ser Gly Thr Asn Asn Tyr Trp Ala Ala His Met Gln Asn Phe
340 345 350
Ser Glu Thr Asn Ser Gly Asn Ile Gln Phe Glu Gly Pro Leu Tyr Gly
355 360 365
Ser Thr Val Gly Thr Ile His Arg Thr Asp Asp Tyr Asp Met Gly Asn
370 375 380
Arg Asp Ile Tyr Thr Ile Thr Ser Gln Ala Val Leu Gly Leu Trp Ala
385 390 395 400
Thr Gly Gln Arg Val Leu Gly Val Ala Ser Ala Arg Phe Thr Leu Arg
405 410 415
Asn Leu Phe Asn Asn Leu Thr Gln Val Leu Val Tyr Glu Asn Pro Ile
420 425 430
Ser Ser Thr Phe Gly Ser Ser Thr Leu Thr His Glu Leu Ser Gly Glu
435 440 445
Asn Ser Asp Arg Pro Thr Ser Ser Asp Tyr Ser His Arg Leu Thr Ser
450 455 460
Ile Thr Gly Phe Arg Ala Gly Ala Asn Gly Thr Val Pro Val Phe Gly
465 470 475 480
Trp Thr Ser Ala Thr Val Asp Arg Asn Asn Ile Ile Glu Arg Asn Lys
485 490 495
Ile Thr Gln Phe Pro Gly Val Lys Ser His Thr Leu Asn Asn Cys Gln
500 505 510
Val Val Arg Gly Thr Gly Phe Thr Gly Gly Asp Trp Leu Arg Pro Asn
515 520 525
Asn Asn Gly Thr Phe Arg Leu Thr Ile Thr Ser Phe Ser Ser Gln Ser
530 535 540
Tyr Arg Ile Arg Leu Arg Tyr Ala Thr Ser Val Gly Asn Thr Ser Leu
545 550 555 560
Val Ile Ser Ser Ser Asp Ala Gly Ile Ser Ser Thr Thr Ile Pro Leu
565 570 575
Thr Ser Thr Ile Thr Ser Leu Pro Gln Thr Val Pro Tyr Gln Ala Phe
580 585 590
Arg Val Val Asp Leu Pro Ile Thr Phe Thr Thr Pro Thr Thr Gln Arg
595 600 605
Asn Tyr Thr Phe Asp Phe Arg Leu Gln Asn Pro Ser Asn Ala Asn Val
610 615 620
Phe Ile Asp Arg Phe Glu Phe Val Pro Ile
625 630
<210> 63
<211> 306
<212> DNA
<213> 苏云金芽孢杆菌
<400> 63
atgacaacaa taaatgaatt atatccggct gtaccttata atgtactggc atatgctcca 60
ccacttaatt tagctgattc gacaccatgg ggtcaaatag ttgttgctga tgcaattaaa 120
gaagcttggg ataattttca aaaatatggt gtattagatt taacagctat aaatcaaggg 180
tttgatgatg caaatacagg ttcttttagt tatcaagctt taatacaaac tgttttgggt 240
attataggta caatttggta tgacagttcc tgtggctgct ccatttgcag ctacagcgcc 300
tattat 306
<210> 64
<211> 4065
<212> DNA
<213> 苏云金芽孢杆菌
<400> 64
atgacagttc ctgtggctgc tccatttgca gctacagcgc ctattattag tttatttgta 60
ggattttttt ggcctaaaaa agataaggga ccacaattaa tcgatataat tgataaagaa 120
attaaaaaat tattagataa ggaattagga gagcaaaaac gtaatgattt agttagtgct 180
ttaaatgaga tgcaagaggg agcaaatgag ttaagtgata ttatgactaa tgcacttttt 240
gaaggtacta tacagggaaa tgttgttact aatgataacc ctcaaggtaa aaggcgaact 300
cctaaagctc caacagttag tgattatgag aatgtttatt cggcatattt tgtggaacat 360
gtggatttta gaaacaaaat atctacgttt cttactggtt cttatgatct tatagcactc 420
ccattatatg cattagcaaa aacaatggag ctttcattgt atcaatcatt tattaatttt 480
gctaataaat ggatggattt tgtatataca aaagcaatta atgaatcagc aactgatgat 540
atgaaaagag attatcaagc gagatacaat actcaaaaaa gtaatttagc tgtacaaaaa 600
acacaattga ttaacaaaat taaagatggt acagatgctg ttatgaaagt ttttaaagat 660
accaataatt taccttcaat aggtactaat aaattagcag taaatgctcg taataagtat 720
attagggcct tacaaataaa ttgtttagat ttagttgctt tgtggcctgg cttatatcca 780
gatgaatatc ttttaccatt acaattagat aaaacacgtg ttgtattttc tgatacaatg 840
ggacctgatg aaacacatga tggtcaaatg aaagttttaa atatattaga ctcaactaca 900
agttataacc atcaagatat aggaataagt acaactcaag atgtaaattc tttattattt 960
tatccaagaa aagaactgtt agaattagat tttgctaaat atatttcatc tagtagtcgt 1020
ttttgggttt atggatttgg cttaaaatat tcagatgata acttttatag atatggtgat 1080
aacgatccaa gcagtgattt taaacctgca tataagtggt ttacgaaaaa ttcccagttc 1140
gaaaaccttc ctacttatgg aaatcctact cctattacta atttaaatgc taaaactcaa 1200
gtaacttctt atcttgatgc attaatatat tatatagacg gaggaactaa tctatataat 1260
aatgcgattc ttcatgatac agggggttat attccgggat atccaggtgt agaaggatat 1320
ggtatgagta ataatgaacc tttagcagga caaaaattaa atgctttata tcctataaaa 1380
gtggaaaatg taagtggttc acaaggaaaa ttaggaacaa tagcagctta tgttccttta 1440
aatttacaac cagaaaatat tattggtgat gctgatccga atacaggttt tccccttaat 1500
gtaattaaag gatttccatt tgaaaaatat ggacctgatt atgagggacg aggaatttcg 1560
gttgtaaaag aatggataaa tggtgcaaat gctgtaaaat tgtctccagg tcaatcagtt 1620
ggggtacaaa ttaaaaatat aacaaaacaa aattatcaaa ttcgtactcg ttatgcaagt 1680
aataacagta atcaagtata ttttaatgta gatccaggtg gatcaccatt atttgcacaa 1740
tcagtaacat ttgaatctac aacaaatgtt acaagtggcc aacaaggcga aaatggtaga 1800
tatacattaa aaactatttt ttctggtaat gatctactta cagtagaaat ccctgttgga 1860
aatttttatg tgcatgttac gaataaagga tcttctgata tctttttaga tcgtcttgag 1920
ttttctacag ttccttcata tgttatatat tcaggtgatt atgatgctac aggtacagat 1980
gatgtcttat tgtcagatcc acatgagtat ttttatgatg tcatagtgaa tggtactgct 2040
agtcattcta gtgcagctac ttctatgaat ttgctcaata aaggaaccgt agtaagaagc 2100
attgatattc caggtcactc aacgtcttat tctgtacagt attcagttcc agaaggattt 2160
gatgaagtta gaattctcag ttctcttccg gatattagtg gaactataag agtagaatct 2220
agtaaaccac ctgtatttaa gaatgatggt aatagtggtg atggtggtaa tactgaatat 2280
aattttaatt ttgatttatc aggattgcaa gatactgggc tttattctgg taaacttaaa 2340
tctggtattc gtgtgcaagg taattacact tacacaggtg ctccatcttt aaatctggtt 2400
gtttacagaa ataatagtgt tgtatccact tttccagtag gttctccttt tgatatcact 2460
ataacaacag aaactgataa ggttatcctt tcattacaac ctcaacatgg gttggcaaca 2520
gttactggta ctggcacaat aacaattcct aatgataaat tagcaattgt ttatgataag 2580
ttatttaaat taccacatga tttagaaaat ataagaatac aagtaaatgc attattcata 2640
tcgagtacac aaaatgaatt agctaaagaa gtaaatgacc atgatattga agaagttgca 2700
ttgaaagtag atgcattatc ggatgaagta tttggaaaag agaaaaaaga attacgtaaa 2760
ctggtcaatc aagcgaaacg tttaagtaaa gcacgaaacc ttctggtagg aggcaatttt 2820
gataattggg aagcttggta taaaggaaaa gaagttgcaa gagtatctga tcatgaatta 2880
ttgaagagtg atcatgtatt attaccgcct ccaactatgt atccatccta tatatatcaa 2940
aaagtagaag aaacaaaatt aaagccaaat actcgttata tgatttctgg tttcatcgca 3000
catgcggaag atttagaaat tgtggtttct cgttatgggc aagaagtaag gaaaatagtg 3060
caagttccat atggagaagc tttcccatta acatccaatg gatcaatttg ttgtacacca 3120
agttttagac gtgatggaaa actatcagat ccacatttct ttagttatag tattgatgta 3180
ggtgaactgg atatgacggc aggtccaggt attgaattgg gacttcgtat tgtagatcga 3240
ttaggaatgg cccgtgtaag taatttagaa attcgtgaag atcgttcttt aacagcaaat 3300
gaaatacgaa aagtgcaacg tatggcaaga aattggagaa ccgaatatga gaaagaacgt 3360
gcagaagtaa cagcattaat tgaacctgta ttaaaccaaa tcaatgcgtt atatgaaaat 3420
ggagattgga atggttctat tcgttcagat atttcgtact acgatataga atctattgta 3480
ttaccaacat taccaagatt acgtcattgg tttgttcctg atatgttaac tgaacatgga 3540
aatatcatga atcgattcga agaagcatta aatcgtgctt atacacagct ggaaggaaat 3600
acactattgc ataacggtca ttttacaaca gatgcggtaa attggatgat acaaggagat 3660
gcacatcagg taatattaga agatggtaga cgtgtattac gattaccaga ctggtcttcg 3720
agtgtatccc aaacaattga aatcgagaaa tttgatccag ataaagaata caacttagta 3780
tttcatgcgc aaggagaagg aacggttacg ttggagcatg gagaaaaaac aaaatatata 3840
gaaacgcata cacatcattt tgcgaatttt acaacatcac aaagtcaagg aattacgttt 3900
gaatcgaata aggtgaccgt ggaaatttct tcagaagatg gggaattatt ggtagatcat 3960
atcgcacttg tggaagttcc tatgtttaac aagaatcaaa tggtcaatga aaatagagat 4020
gtaaatataa atagcaatac aaatatgaat aatagcaata atcaa 4065
<210> 65
<211> 4772
<212> DNA
<213> 苏云金芽孢杆菌
<400> 65
tacccttcat atagtataaa gggacaacct cctatttata tatatattat tgtaattaca 60
ttatagaaga tcaaacagaa aaaagttttt tgaattggtt caatatacca actatatagc 120
ataaactatc tttgtattta tttataataa ttttttaagg agtgaaataa tgacaacaat 180
aaatgaatta tatccggctg taccttataa tgtactggca tatgctccac cacttaattt 240
agctgattcg acaccatggg gtcaaatagt tgttgctgat gcaattaaag aagcttggga 300
taattttcaa aaatatggtg tattagattt aacagctata aatcaagggt ttgatgatgc 360
aaatacaggt tcttttagtt atcaagcttt aatacaaact gttttgggta ttataggtac 420
aatttggtat gacagttcct gtggctgctc catttgcagc tacagcgcct attattagtt 480
tatttgtagg atttttttgg cctaaaaaag ataagggacc acaattaatc gatataattg 540
ataaagaaat taaaaaatta ttagataagg aattaggaga gcaaaaacgt aatgatttag 600
ttagtgcttt aaatgagatg caagagggag caaatgagtt aagtgatatt atgactaatg 660
cactttttga aggtactata cagggaaatg ttgttactaa tgataaccct caaggtaaaa 720
ggcgaactcc taaagctcca acagttagtg attatgagaa tgtttattcg gcatattttg 780
tggaacatgt ggattttaga aacaaaatat ctacgtttct tactggttct tatgatctta 840
tagcactccc attatatgca ttagcaaaaa caatggagct ttcattgtat caatcattta 900
ttaattttgc taataaatgg atggattttg tatatacaaa agcaattaat gaatcagcaa 960
ctgatgatat gaaaagagat tatcaagcga gatacaatac tcaaaaaagt aatttagctg 1020
tacaaaaaac acaattgatt aacaaaatta aagatggtac agatgctgtt atgaaagttt 1080
ttaaagatac caataattta ccttcaatag gtactaataa attagcagta aatgctcgta 1140
ataagtatat tagggcctta caaataaatt gtttagattt agttgctttg tggcctggct 1200
tatatccaga tgaatatctt ttaccattac aattagataa aacacgtgtt gtattttctg 1260
atacaatggg acctgatgaa acacatgatg gtcaaatgaa agttttaaat atattagact 1320
caactacaag ttataaccat caagatatag gaataagtac aactcaagat gtaaattctt 1380
tattatttta tccaagaaaa gaactgttag aattagattt tgctaaatat atttcatcta 1440
gtagtcgttt ttgggtttat ggatttggct taaaatattc agatgataac ttttatagat 1500
atggtgataa cgatccaagc agtgatttta aacctgcata taagtggttt acgaaaaatt 1560
cccagttcga aaaccttcct acttatggaa atcctactcc tattactaat ttaaatgcta 1620
aaactcaagt aacttcttat cttgatgcat taatatatta tatagacgga ggaactaatc 1680
tatataataa tgcgattctt catgatacag ggggttatat tccgggatat ccaggtgtag 1740
aaggatatgg tatgagtaat aatgaacctt tagcaggaca aaaattaaat gctttatatc 1800
ctataaaagt ggaaaatgta agtggttcac aaggaaaatt aggaacaata gcagcttatg 1860
ttcctttaaa tttacaacca gaaaatatta ttggtgatgc tgatccgaat acaggttttc 1920
cccttaatgt aattaaagga tttccatttg aaaaatatgg acctgattat gagggacgag 1980
gaatttcggt tgtaaaagaa tggataaatg gtgcaaatgc tgtaaaattg tctccaggtc 2040
aatcagttgg ggtacaaatt aaaaatataa caaaacaaaa ttatcaaatt cgtactcgtt 2100
atgcaagtaa taacagtaat caagtatatt ttaatgtaga tccaggtgga tcaccattat 2160
ttgcacaatc agtaacattt gaatctacaa caaatgttac aagtggccaa caaggcgaaa 2220
atggtagata tacattaaaa actatttttt ctggtaatga tctacttaca gtagaaatcc 2280
ctgttggaaa tttttatgtg catgttacga ataaaggatc ttctgatatc tttttagatc 2340
gtcttgagtt ttctacagtt ccttcatatg ttatatattc aggtgattat gatgctacag 2400
gtacagatga tgtcttattg tcagatccac atgagtattt ttatgatgtc atagtgaatg 2460
gtactgctag tcattctagt gcagctactt ctatgaattt gctcaataaa ggaaccgtag 2520
taagaagcat tgatattcca ggtcactcaa cgtcttattc tgtacagtat tcagttccag 2580
aaggatttga tgaagttaga attctcagtt ctcttccgga tattagtgga actataagag 2640
tagaatctag taaaccacct gtatttaaga atgatggtaa tagtggtgat ggtggtaata 2700
ctgaatataa ttttaatttt gatttatcag gattgcaaga tactgggctt tattctggta 2760
aacttaaatc tggtattcgt gtgcaaggta attacactta cacaggtgct ccatctttaa 2820
atctggttgt ttacagaaat aatagtgttg tatccacttt tccagtaggt tctccttttg 2880
atatcactat aacaacagaa actgataagg ttatcctttc attacaacct caacatgggt 2940
tggcaacagt tactggtact ggcacaataa caattcctaa tgataaatta gcaattgttt 3000
atgataagtt atttaaatta ccacatgatt tagaaaatat aagaatacaa gtaaatgcat 3060
tattcatatc gagtacacaa aatgaattag ctaaagaagt aaatgaccat gatattgaag 3120
aagttgcatt gaaagtagat gcattatcgg atgaagtatt tggaaaagag aaaaaagaat 3180
tacgtaaact ggtcaatcaa gcgaaacgtt taagtaaagc acgaaacctt ctggtaggag 3240
gcaattttga taattgggaa gcttggtata aaggaaaaga agttgcaaga gtatctgatc 3300
atgaattatt gaagagtgat catgtattat taccgcctcc aactatgtat ccatcctata 3360
tatatcaaaa agtagaagaa acaaaattaa agccaaatac tcgttatatg atttctggtt 3420
tcatcgcaca tgcggaagat ttagaaattg tggtttctcg ttatgggcaa gaagtaagga 3480
aaatagtgca agttccatat ggagaagctt tcccattaac atccaatgga tcaatttgtt 3540
gtacaccaag ttttagacgt gatggaaaac tatcagatcc acatttcttt agttatagta 3600
ttgatgtagg tgaactggat atgacggcag gtccaggtat tgaattggga cttcgtattg 3660
tagatcgatt aggaatggcc cgtgtaagta atttagaaat tcgtgaagat cgttctttaa 3720
cagcaaatga aatacgaaaa gtgcaacgta tggcaagaaa ttggagaacc gaatatgaga 3780
aagaacgtgc agaagtaaca gcattaattg aacctgtatt aaaccaaatc aatgcgttat 3840
atgaaaatgg agattggaat ggttctattc gttcagatat ttcgtactac gatatagaat 3900
ctattgtatt accaacatta ccaagattac gtcattggtt tgttcctgat atgttaactg 3960
aacatggaaa tatcatgaat cgattcgaag aagcattaaa tcgtgcttat acacagctgg 4020
aaggaaatac actattgcat aacggtcatt ttacaacaga tgcggtaaat tggatgatac 4080
aaggagatgc acatcaggta atattagaag atggtagacg tgtattacga ttaccagact 4140
ggtcttcgag tgtatcccaa acaattgaaa tcgagaaatt tgatccagat aaagaataca 4200
acttagtatt tcatgcgcaa ggagaaggaa cggttacgtt ggagcatgga gaaaaaacaa 4260
aatatataga aacgcataca catcattttg cgaattttac aacatcacaa agtcaaggaa 4320
ttacgtttga atcgaataag gtgaccgtgg aaatttcttc agaagatggg gaattattgg 4380
tagatcatat cgcacttgtg gaagttccta tgtttaacaa gaatcaaatg gtcaatgaaa 4440
atagagatgt aaatataaat agcaatacaa atatgaataa tagcaataat caataacttt 4500
tatatgtaaa caggtgcaag tgtttgttgc acctgttttt tcaccctgtt tctaaaaaaa 4560
tgaggaggtt agatgtatgc aaaagaaatc aaaagtaata gaaccaacaa ataatagtat 4620
gtcagtaaat aataatattt cattaaaaac accgatgcca attgggtata gaaaaaaatc 4680
aggttgtggt tgtgggaaac gtcgttaagt ggaaaatgaa tctgtggaat ggataaaaac 4740
aagtgtgaaa gttgaacgtt catccaattt ac 4772
<210> 66
<211> 723
<212> DNA
<213> 苏云金芽孢杆菌
<400> 66
atggccgata tgcctgtaaa taatactcat atatcacaaa atgattttcc aatctgtaca 60
actgaagaag aacgtttaat tccattttgt tgtttggttc agctaccaca tgattttcaa 120
cttgttcctt attgcaaacc acgtcttgta tacaacattg gatgccttgg aacgactaaa 180
gaaacgtgta aaaaaaccat acaagtagag gattgtggac aaacggaaat tgatttgcag 240
atattaaagg caaaaggatg catcaccttt cttgtgaata tagatgtaga acctgtctgt 300
gaagaagaga tttgctcgag tgttccgcac acaaaggaca tgattttgtg ttgtaaagga 360
accgtatgtg tagacaaaat tttaaaatgc agtgttgact gtttacctga tattcattta 420
gattgtgaga atgtaaaagt ttgtgattta caagtaaaat cactctgcga ggaagattgt 480
cattctgtaa agattacagg gtattttcag atttgtattg attaaataaa gatttttaaa 540
tgcatacttt tttacatcca ctcaaataga gagagtgttg caaggttaaa aagcaatatg 600
tttttataaa tatttattta tttcttttgt cctggtgcag aaaaataaaa taatttcaaa 660
atgatttttc taactttgcc ataatactac taaaaaggtg atagagagac taagaatgat 720
aag 723
<210> 67
<211> 174
<212> PRT
<213> 苏云金芽孢杆菌
<400> 67
Met Ala Asp Met Pro Val Asn Asn Thr His Ile Ser Gln Asn Asp Phe
1 5 10 15
Pro Ile Cys Thr Thr Glu Glu Glu Arg Leu Ile Pro Phe Cys Cys Leu
20 25 30
Val Gln Leu Pro His Asp Phe Gln Leu Val Pro Tyr Cys Lys Pro Arg
35 40 45
Leu Val Tyr Asn Ile Gly Cys Leu Gly Thr Thr Lys Glu Thr Cys Lys
50 55 60
Lys Thr Ile Gln Val Glu Asp Cys Gly Gln Thr Glu Ile Asp Leu Gln
65 70 75 80
Ile Leu Lys Ala Lys Gly Cys Ile Thr Phe Leu Val Asn Ile Asp Val
85 90 95
Glu Pro Val Cys Glu Glu Glu Ile Cys Ser Ser Val Pro His Thr Lys
100 105 110
Asp Met Ile Leu Cys Cys Lys Gly Thr Val Cys Val Asp Lys Ile Leu
115 120 125
Lys Cys Ser Val Asp Cys Leu Pro Asp Ile His Leu Asp Cys Glu Asn
130 135 140
Val Lys Val Cys Asp Leu Gln Val Lys Ser Leu Cys Glu Glu Asp Cys
145 150 155 160
His Ser Val Lys Ile Thr Gly Tyr Phe Gln Ile Cys Ile Asp
165 170
<210> 68
<211> 589
<212> DNA
<213> 苏云金芽孢杆菌
<400> 68
atgacatttg attacgagga agaagaaagg gatttgtccc aactccgacc atctgattct 60
tcaagatcaa caggtgaacc aagaacggat tgtgacctaa ccacaaattg tgaagttcct 120
ttttgttgcg tcgtcacgct cccacatgga tttcagtacg aatcccgaaa acaaacaaag 180
cttgtatacg atattagttg tcttaccttt gcacacgaga tgtgtcaacg atctatcaac 240
gtggatcagt gtggtacagt tgatgtagat ttacaagtct taaaaataaa gggctgtgtg 300
tcactgtata tcaacgttcc gattctaccc attcgtgagg agacgatgtg tacgttacac 360
agacagccga catctctcta tacctgttgt caggataccc tttgtgtaga tcatattgtg 420
aaatgtagtg tgggaagtct cccttactac gtgttggatg gcaatcatat acaagtgtgt 480
gacttacagg tacgtcctgt atcagaagta catccacacg tattacaagt atccggtcgt 540
tttgagtttt tatatacgta agtatctata tgaggagtga gataaagat 589
<210> 69
<211> 186
<212> PRT
<213> 苏云金芽孢杆菌
<400> 69
Met Thr Phe Asp Tyr Glu Glu Glu Glu Arg Asp Leu Ser Gln Leu Arg
1 5 10 15
Pro Ser Asp Ser Ser Arg Ser Thr Gly Glu Pro Arg Thr Asp Cys Asp
20 25 30
Leu Thr Thr Asn Cys Glu Val Pro Phe Cys Cys Val Val Thr Leu Pro
35 40 45
His Gly Phe Gln Tyr Glu Ser Arg Lys Gln Thr Lys Leu Val Tyr Asp
50 55 60
Ile Ser Cys Leu Thr Phe Ala His Glu Met Cys Gln Arg Ser Ile Asn
65 70 75 80
Val Asp Gln Cys Gly Thr Val Asp Val Asp Leu Gln Val Leu Lys Ile
85 90 95
Lys Gly Cys Val Ser Leu Tyr Ile Asn Val Pro Ile Leu Pro Ile Arg
100 105 110
Glu Glu Thr Met Cys Thr Leu His Arg Gln Pro Thr Ser Leu Tyr Thr
115 120 125
Cys Cys Gln Asp Thr Leu Cys Val Asp His Ile Val Lys Cys Ser Val
130 135 140
Gly Ser Leu Pro Tyr Tyr Val Leu Asp Gly Asn His Ile Gln Val Cys
145 150 155 160
Asp Leu Gln Val Arg Pro Val Ser Glu Val His Pro His Val Leu Gln
165 170 175
Val Ser Gly Arg Phe Glu Phe Leu Tyr Thr
180 185
<210> 70
<211> 4
<212> PRT
<213> 人工序列
<220>
<223> ER靶向肽
<400> 70
Lys Asp Glu Leu
1

Claims (16)

1.重组的核酸分子，其选自以下的核苷酸序列且编码的多肽具有杀虫活性：
a)任一SEQ ID NO:15、1-6、8-14和16-27的核苷酸序列或者其互补序列；和
b)编码任一SEQ ID NO:44、28-33、36-43和45-61的氨基酸序列的多肽的核苷酸序列。
2.权利要求1所述的重组核酸分子，其中所述的核苷酸序列是经设计用于在植物中表达的合成序列。
3.权利要求1所述的重组核酸分子，其中所述的核苷酸序列与能指导所述核苷酸序列在植物细胞中表达的启动子有效连接。
4.表达盒，其包含权利要求3所述的重组核苷酸序列。
5.权利要求4所述的表达盒，其进一步包含编码异源多肽的核酸分子。
6.细菌宿主细胞，其含有权利要求4所述的表达盒。
7.具有杀虫活性的重组多肽，其选自任一SEQ ID NO:44、28-33、36-43和45-61的氨基酸序列的多肽。
8.一种多肽，其包含任一SEQ ID NO:44、28-33、36-43和45-61的氨基酸序列和异源氨基酸序列。
9.组合物，其包含权利要求7所述的多肽。
10.权利要求9所述的组合物，其中所述组合物选自粉剂、尘剂、丸剂、颗粒剂、喷雾剂、乳剂、胶体剂和溶液剂。
11.权利要求9所述的组合物，其中所述的组合物通过干燥、冻干、匀化、提取、过滤、离心、沉淀或浓缩苏云金芽孢杆菌(Bacillus thuringiensis)细胞培养物来制备。
12.权利要求9所述的组合物，其包含以重量计1％至99％的所述多肽。
13.用于控制鳞翅目害虫种群的方法，包括使所述种群与杀虫有效量的权利要求7的多肽接触。
14.用于矮化鳞翅目的害虫的方法，包括使所述害虫与杀虫有效量的权利要求7的多肽接触或向所述害虫饲喂杀虫有效量的权利要求7的多肽。
15.用于产生具有杀虫活性的多肽的方法，包括在编码所述多肽的核酸分子表达的条件下培养权利要求6的宿主细胞。
16.用于保护植物免遭害虫侵害的方法，包括在植物或其细胞中表达至少一个表达载体，所述至少一个表达载体包含编码杀虫多肽的核苷酸序列，其中所述的核苷酸序列选自：
a)任一SEQ ID NO:15、1-6、8-14和16-27所述的核苷酸序列；和
b)编码任一SEQ ID NO:44、28-33、36-43和45-61的氨基酸序列的多肽的核苷酸序列。
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021168309A1 (en) * 2020-02-21 2021-08-26 BASF Agricultural Solutions Seed US LLC Toxin gene and methods for its use

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX367427B (es) * 2011-04-05 2019-08-21 Athenix Corp Gen insecticida variante axmi115 y metodos para utilizarlo.
CN103958531B (zh) 2011-11-21 2016-12-28 拜耳知识产权有限责任公司 杀真菌剂n‑[(三取代的甲硅烷基)甲基]‑羧酰胺衍生物
AR089656A1 (es) 2011-11-30 2014-09-10 Bayer Ip Gmbh Derivados de n-bicicloalquil- y n-tricicloalquil-(tio)-carboxamida fungicidas
IN2014DN06122A (zh) 2011-12-29 2015-08-14 Bayer Ip Gmbh
US9556158B2 (en) 2011-12-29 2017-01-31 Bayer Intellectual Property Gmbh Fungicidal 3-[(pyridin-2-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2H)-one derivatives
WO2014036238A1 (en) * 2012-08-30 2014-03-06 Athenix Corp. Axmi-234 and axmi-235 toxin genes and methods for their use
CN105357968A (zh) 2012-10-19 2016-02-24 拜尔农科股份公司 包含羧酰胺衍生物的活性化合物复配物
PT2908641T (pt) 2012-10-19 2018-04-16 Bayer Cropscience Ag Método para o tratamento de plantas contra fungos resistentes a fungicidas utilizando derivados de carboxamida ou tiocarboxamida
JP6262747B2 (ja) 2012-10-19 2018-01-17 バイエル・クロップサイエンス・アクチェンゲゼルシャフト カルボキサミド誘導体を用いる植物成長促進方法
WO2014060519A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives
BR112015026235A2 (pt) 2013-04-19 2017-10-10 Bayer Cropscience Ag método para melhorar a utilização do potencial de produção de plantas transgênicas envolvendo a aplicação de um derivado de ftaldiamida
TW201507722A (zh) 2013-04-30 2015-03-01 Bayer Cropscience Ag 做為殺線蟲劑及殺體內寄生蟲劑的ｎ－（２－鹵素－２－苯乙基）－羧醯胺類
WO2014177514A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag Nematicidal n-substituted phenethylcarboxamides
WO2014182658A1 (en) * 2013-05-06 2014-11-13 Abbvie Inc. Compositions for cell culture and methods of using the same
UY35696A (es) 2013-08-09 2015-03-27 Athenix Corp ?molécula de adn recombinante que comprende al gen de toxina axmi440, vector, célula huésped, plantas, composiciones y métodos relacionados?.
BR122021024287B1 (pt) * 2013-12-09 2022-10-11 Athenix Corp Constructo, vetor, célula hospedeira bacteriana, polipeptídeo, composição, método de controle e exterminação de praga lepidóptera, método de proteção de planta e método de produção de polipeptídeo com atividade pesticida
CU24405B1 (es) 2014-10-16 2019-05-03 Monsanto Technology Llc Proteínas de variantes de secuencias de aminoácidos de cry1da1 activas para lepidópteros
CU24456B1 (es) 2014-10-16 2019-11-04 Monsanto Technology Llc Proteínas quiméricas insecticidas tóxicas o inhibidoras de plagas de lepidópteros
US10316329B2 (en) 2014-10-16 2019-06-11 Monsanto Technology Llc Proteins toxic or inhibitory to lepidopteran insects
US10487123B2 (en) 2014-10-16 2019-11-26 Monsanto Technology Llc Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
US11130964B2 (en) * 2014-11-20 2021-09-28 Monsanto Technology Llc Insect inhibitory proteins
BR122020006755B1 (pt) * 2014-11-20 2024-01-23 Monsanto Technology Llc Cassete de expressão, célula hospedeira bacteriana, composição inibidora de inseto, método para controlar uma praga de espécie coleoptera, e vetor recombinante
CN104845913B (zh) * 2015-05-26 2018-04-06 中国农业科学院植物保护研究所 苏云金芽胞杆菌菌株，组合蛋白及其应用
CN104845914B (zh) * 2015-05-26 2018-04-06 中国农业科学院植物保护研究所 苏云金芽胞杆菌菌株，表达蛋白及其应用
MA49662A (fr) * 2015-06-22 2020-06-03 Agbiome Inc Gènes pesticides et leurs procédés d'utilisation
MX2018001408A (es) 2015-08-07 2018-03-15 Bayer Cropscience Nv Uso de anexina para para mejorar el rendimiento en condiciones de estres en plantas.
US10036037B2 (en) 2015-08-18 2018-07-31 Monsanto Technology Llc Insect inhibitory proteins
EP3349582B1 (en) 2015-08-27 2021-08-11 Monsanto Technology LLC Novel insect inhibitory proteins
US10435707B2 (en) * 2015-10-14 2019-10-08 Basf Agricultural Solutions Seed, Us Llc AXMI554 delta-endotoxin gene and methods for its use
MX2018013249A (es) 2016-05-04 2019-02-13 Pioneer Hi Bred Int Proteinas insecticidas y metodos para sus usos.
CA3057145A1 (en) * 2017-04-03 2018-10-11 Monsanto Technology Llc Novel insect inhibitory proteins
JP2021525774A (ja) 2018-06-04 2021-09-27 バイエル アクチェンゲゼルシャフトＢａｙｅｒ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 除草活性二環式ベンゾイルピラゾール
CN109867710B (zh) * 2018-12-20 2022-03-15 河南工业大学 一种新型广谱抗菌肽samp1-a3及其制备方法
CN109929015B (zh) * 2019-04-17 2021-08-31 东北农业大学 苏云金芽胞杆菌杀虫基因cry79Aa1、表达蛋白及其应用
BR112022007119A2 (pt) * 2019-10-14 2022-07-05 BASF Agricultural Solutions Seed US LLC Molécula de ácido nucleico, ácido nucleico, polipeptídeos, vetor, célula hospedeira, planta transgênica, semente transgênica, composição, métodos para controlar uma população de pragas, para matar uma praga, para produzir um polipeptídeo, para proteger uma planta e para aumentar o rendimento em uma planta, planta, uso do ácido nucleico e produto básico
AU2020367523A1 (en) * 2019-10-14 2022-04-28 BASF Agricultural Solutions Seed US LLC Novel insect resistant genes and methods of use
US20230235352A1 (en) 2020-07-14 2023-07-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN112210506B (zh) * 2020-07-17 2021-05-28 江西省农业科学院农业应用微生物研究所（江西省农村能源研究中心） 一株对灵芝谷蛾有杀虫活性的苏云金芽孢杆菌jxbt-0350及其应用
WO2022125639A1 (en) 2020-12-08 2022-06-16 Monsanto Technology Llc Modified plant-associated bacteria and methods of their use
CN116670155A (zh) 2020-12-21 2023-08-29 孟山都技术公司 新型昆虫抑制性蛋白质
UY39585A (es) 2020-12-23 2022-07-29 Monsanto Technology Llc Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas
US11673922B2 (en) 2020-12-31 2023-06-13 Monsanto Technology Llc Insect inhibitory proteins

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3758383A (en) * 1971-06-01 1973-09-11 Int Minerals & Chem Corp Production of exotoxin of bacillus thuringiensis
US5262158A (en) * 1991-04-30 1993-11-16 Mycogen Corporation Bacillus thuringiensis isolates for controlling acarida
EP0325037B1 (en) * 1987-12-24 1994-04-06 Zeneca Limited Bacterial strains
CN1245502A (zh) * 1996-11-27 2000-02-23 艾可根公司 编码鳞翅目活性的δ－内毒素的DNA和其用途
US20010010932A1 (en) * 1987-08-12 2001-08-02 Mycogen Corporation Nematicidal proteins
CN1390259A (zh) * 1999-09-15 2003-01-08 孟山都技术有限公司 对鳞翅目昆虫有活性的苏云金芽孢杆菌δ内毒素组合物及其使用方法
US20050049410A1 (en) * 2003-08-28 2005-03-03 Athenix Corporation AXMI-003, a delta-endotoxin gene and methods for its use
CN101054589A (zh) * 2004-11-16 2007-10-17 中国农业科学院植物保护研究所 对鞘翅目害虫高效的cry8F基因、其表达蛋白及其应用

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4196265A (en) 1977-06-15 1980-04-01 The Wistar Institute Method of producing antibodies
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
EP0482125A1 (en) 1989-07-11 1992-04-29 Biotechnology Research And Development Corporation Aerosol beam microinjection
US5240842A (en) 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
CA2051562C (en) 1990-10-12 2003-12-02 Jewel M. Payne Bacillus thuringiensis isolates active against dipteran pests
TW261517B (zh) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
US5849870A (en) 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6468523B1 (en) 1998-11-02 2002-10-22 Monsanto Technology Llc Polypeptide compositions toxic to diabrotic insects, and methods of use
US6938976B2 (en) 1999-06-16 2005-09-06 Eastman Kodak Company Printer and method therefor adapted to sense data uniquely associated with a consumable loaded into the printer
ATE538205T1 (de) 1999-11-29 2012-01-15 Midwest Oilseeds Inc Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen
AU2003218114B2 (en) 2002-03-11 2008-09-18 Athenix Corporation Integrated system for high throughput capture of genetic diversity
ATE412054T1 (de) 2003-02-20 2008-11-15 Athenix Corp Delta-endotoxin gene und verfahren zu ihrer verwendung
AU2006210880B2 (en) * 2005-01-31 2012-09-20 Athenix Corporation AXMI-018, AXMI-020, and AXMI-021, a family of delta-endotoxin genes and methods for their use
EP2455394B1 (en) 2006-06-15 2017-04-12 Athenix Corporation A family of pesticidal proteins and methods for their use
CA2728622C (en) * 2008-07-02 2023-02-28 Athenix Corp. Axmi-115, axmi-113, axmi-005, axmi-163 and axmi-184: insecticidal proteins and methods for their use
EP2361307B1 (en) * 2008-12-22 2014-09-24 Athenix Corporation Pesticidal genes from Brevibacillus and methods for their use

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3758383A (en) * 1971-06-01 1973-09-11 Int Minerals & Chem Corp Production of exotoxin of bacillus thuringiensis
US20010010932A1 (en) * 1987-08-12 2001-08-02 Mycogen Corporation Nematicidal proteins
EP0325037B1 (en) * 1987-12-24 1994-04-06 Zeneca Limited Bacterial strains
US5262158A (en) * 1991-04-30 1993-11-16 Mycogen Corporation Bacillus thuringiensis isolates for controlling acarida
CN1245502A (zh) * 1996-11-27 2000-02-23 艾可根公司 编码鳞翅目活性的δ－内毒素的DNA和其用途
CN1390259A (zh) * 1999-09-15 2003-01-08 孟山都技术有限公司 对鳞翅目昆虫有活性的苏云金芽孢杆菌δ内毒素组合物及其使用方法
US20050049410A1 (en) * 2003-08-28 2005-03-03 Athenix Corporation AXMI-003, a delta-endotoxin gene and methods for its use
CN101054589A (zh) * 2004-11-16 2007-10-17 中国农业科学院植物保护研究所 对鞘翅目害虫高效的cry8F基因、其表达蛋白及其应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JING XUE等: "Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects", 《FEMS MICROBIOLOGY LETTERS》 *
伍赠玲等: "苏云金芽孢杆菌杀虫蛋白基因定位研究进展", 《福建农业大学学报》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021168309A1 (en) * 2020-02-21 2021-08-26 BASF Agricultural Solutions Seed US LLC Toxin gene and methods for its use

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