ATE538205T1 - Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen - Google Patents

Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen

Info

Publication number
ATE538205T1
ATE538205T1 AT00980823T AT00980823T ATE538205T1 AT E538205 T1 ATE538205 T1 AT E538205T1 AT 00980823 T AT00980823 T AT 00980823T AT 00980823 T AT00980823 T AT 00980823T AT E538205 T1 ATE538205 T1 AT E538205T1
Authority
AT
Austria
Prior art keywords
bacteria
media
plant cells
introducing molecules
aerosol jets
Prior art date
Application number
AT00980823T
Other languages
English (en)
Inventor
Bruce Held
Herbert Wilson
Liming Hou
Carol Lewnau
Janelle Eby
Original Assignee
Midwest Oilseeds Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Midwest Oilseeds Inc filed Critical Midwest Oilseeds Inc
Application granted granted Critical
Publication of ATE538205T1 publication Critical patent/ATE538205T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C12N15/895—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
AT00980823T 1999-11-29 2000-11-28 Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen ATE538205T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45022699A 1999-11-29 1999-11-29
PCT/US2000/032362 WO2001038514A2 (en) 1999-11-29 2000-11-28 Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams

Publications (1)

Publication Number Publication Date
ATE538205T1 true ATE538205T1 (de) 2012-01-15

Family

ID=23787260

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00980823T ATE538205T1 (de) 1999-11-29 2000-11-28 Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen

Country Status (8)

Country Link
US (6) US6809232B1 (de)
EP (1) EP1250447B1 (de)
AR (1) AR026645A1 (de)
AT (1) ATE538205T1 (de)
AU (1) AU1803601A (de)
BR (1) BR0016030A (de)
CA (1) CA2396392C (de)
WO (1) WO2001038514A2 (de)

Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
DE19926216A1 (de) * 1999-06-09 2001-02-22 Metallgesellschaft Ag Verfahren zur Herstellung von Bariumsulfat, Bariumsulfat und Verwendung des Bariumsulfats
EP1250447B1 (de) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Verfahren, Medien und Vorrichtung zur Einführung von Molekülen in Pflanzenzellen und Bakterien mittels Aerosolstrahlen
US7314969B2 (en) 1999-11-29 2008-01-01 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
EP1313868B1 (de) * 2000-08-30 2006-07-19 North Carolina State University Transgene pflanzen, die molekulare decoys enthalten, die den proteininhalt ändern
CN1258593C (zh) * 2000-11-07 2006-06-07 北卡罗莱纳州立大学 腐胺－n－甲基转移酶启动子
AU3934302A (en) * 2000-11-28 2002-06-11 Midwest Oilseeds Inc Methods and compositions for the introduction of molecules into cells
CN110066826A (zh) 2001-05-31 2019-07-30 波夫曼斯种植公司 增强植物中胁迫耐受的组合物和方法
US20060157072A1 (en) * 2001-06-08 2006-07-20 Anthony Albino Method of reducing the harmful effects of orally or transdermally delivered nicotine
MXPA03011385A (es) * 2001-06-08 2005-03-07 Vector Tobacco Ltd Modeificacion de los niveles de nicotina y nitrosamina en el tabaco.
ES2316963T3 (es) 2003-02-20 2009-04-16 Athenix Corporation Genes delta-entotoxino y los metodos para su uso.
ES2407857T5 (es) 2004-04-30 2017-07-31 Dow Agrosciences Llc Nuevo gen de resistencia a los herbicidas
WO2006107761A2 (en) 2005-04-01 2006-10-12 Athenix Corporation Axmi-027, axmi-036 and axmi-038, a family of delta-endotoxin genes and methods for their use
JP4879969B2 (ja) 2005-04-08 2012-02-22 アセニクス コーポレイション 新規なクラスのｅｐｓｐシンターゼの同定
DK2484202T3 (en) 2005-10-28 2017-09-11 Dow Agrosciences Llc NEW HERBICID RESISTANCE GENES
US7674958B2 (en) 2005-12-01 2010-03-09 Athenix Corporation GRG23 and GRG51 genes conferring herbicide resistance
US20080313769A9 (en) 2006-01-12 2008-12-18 Athenix Corporation EPSP synthase domains conferring glyphosate resistance
US20070173470A1 (en) * 2006-01-23 2007-07-26 Chi-Hung Lin Methods for delivering extracellular target into cells
WO2007103768A2 (en) 2006-03-02 2007-09-13 Athenix Corporation Methods and compositions for improved enzyme activity in transgenic plant
EP2455392A3 (de) 2006-06-15 2012-08-22 Athenix Corporation Pestizidproteinfamilie und Verwendungsverfahren dafür
KR101413124B1 (ko) * 2006-10-02 2014-07-01 필립모리스 프로덕츠 에스.에이. 연속적인 고압 전달 시스템
WO2009027824A2 (en) 2007-04-18 2009-03-05 Performance Plants, Inc. Plants having increased tolerance to heat stress
MX2009012120A (es) 2007-05-09 2010-02-12 Dow Agrosciences Llc Genes novedosos con resistencia a herbicidas.
AR068542A1 (es) * 2007-09-24 2009-11-18 Performance Plants Inc Plantas que tienen biomasa aumentada
MX2010003438A (es) 2007-10-02 2010-04-21 Philip Morris Prod Sistema capilar con elemento fluidico.
US20090126044A1 (en) 2007-10-10 2009-05-14 Athenix Corporation Synthetic genes encoding cry1ac
US20090144852A1 (en) 2007-10-16 2009-06-04 Athenix Corporation Axmi-066 and axmi-076: delta-endotoxin proteins and methods for their use
CN102203261B (zh) 2008-06-13 2017-05-24 波夫曼斯种植公司 提高植物水分利用效率的方法和手段
MX351227B (es) 2008-06-25 2017-09-14 Athenix Corp Genes de toxinas y métodos para su uso.
MX357562B (es) 2008-07-02 2018-07-13 Athenix Corp Axmi-115, axmi-113, axmi-005, axmi-163 y axmi-184: proteinas insecticidas vip3a de bacillus thuringiensis y metodos para su uso.
AR074672A1 (es) 2008-12-15 2011-02-02 Athenix Corp Genes que codifican toxinas contra nematodos
BRPI0922431A2 (pt) 2008-12-22 2015-08-04 Athenix Corp Genes pesticidas de brevibacillus e métodos para sua utilização
ES2534581T3 (es) 2008-12-23 2015-04-24 Athenix Corporation Gen AXMI-150 de la delta-endotoxina y métodos para su uso
WO2011068567A1 (en) 2009-07-10 2011-06-09 Syngenta Participations Ag Novel hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
BRPI1007175A2 (pt) 2009-01-22 2015-08-18 Syngenta Participations Ag Polipeptídeos hidroxifenilpriruvato dioxigenase mutantes e métodos de uso
WO2010091230A1 (en) 2009-02-05 2010-08-12 Athenix Corporation Variant axmi-r1 delta-endotoxin genes and methods for their use
BRPI1008740A8 (pt) 2009-02-27 2022-07-05 Athenix Corp Proteínas pesticidas e métodos para seu uso.
BRPI1013362A2 (pt) 2009-03-06 2015-09-01 Athenix Corp Métodos e composições para controle de pestes de planta.
WO2010141141A2 (en) 2009-03-11 2010-12-09 Athenix Corporation Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: toxin genes and methods for their use
US8575425B2 (en) 2009-07-02 2013-11-05 Athenix Corporation AXMI-205 pesticidal gene and methods for its use
CA2769643C (en) 2009-07-31 2020-01-07 Athenix Corp. Axmi-192 family of pesticidal genes and methods for their use
AU2011218131B2 (en) 2010-02-18 2016-03-17 BASF Agricultural Solutions Seed US LLC AXMI221z, AXMI222z, AXMI223z, AXMI224z, and AXMI225z delta-endotoxin genes and methods for their use
CA2790023A1 (en) 2010-02-18 2011-08-25 Athenix Corp. Axmi218, axmi219, axmi220, axmi226, axmi227, axmi228, axmi229, axmi230, and axmi231 delta-endotoxin genes and methods for their use
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
CA2823237C (en) 2010-12-29 2020-12-29 Syngenta Participations Ag Methods and compositions for a soybean in-planta transient expression system
US8648230B2 (en) 2011-03-18 2014-02-11 Ms Technologies, Llc Regulatory regions preferentially expressing in non-pollen plant tissue
WO2012135501A2 (en) 2011-03-30 2012-10-04 Athenix Corp. Axmi232, axmi233, and axmi249 toxin genes and methods for their use
WO2012135436A1 (en) 2011-03-30 2012-10-04 Athenix Corp. Axmi238 toxin gene and methods for its use
EP3971297A1 (de) 2011-04-05 2022-03-23 BASF Agricultural Solutions Seed US LLC Insektizides gen der axmi115-variante und verfahren zu dessen verwendung
ES2657825T3 (es) 2011-06-06 2018-03-07 Bayer Cropscience Nv Métodos y medios para modificar el genoma de una planta en un sitio preseleccionado
CA2844357A1 (en) 2011-07-28 2013-01-31 Athenix Corp. Axmi270 toxin gene, peptides, compositions, host cells and methods thereof
WO2013016617A1 (en) 2011-07-28 2013-01-31 Athenix Corp. Axmi205 variant proteins and methods for their use
CN103975065B (zh) 2011-07-29 2018-08-10 阿森尼克斯公司 Axmi279杀虫基因及其使用方法
US8759618B2 (en) 2011-08-17 2014-06-24 Stine Seed Farm, Inc. Maize event HCEM485, compositions and methods for detecting and use thereof
MX2014002027A (es) 2011-08-19 2014-11-10 Synthetic Genomics Inc Metodo integrado para identificacion de alto rendimiento de composiciones pesticidas novedosas y uso para el mismo.
US10538774B2 (en) 2011-08-22 2020-01-21 Basf Agricultural Solutions Seed, Us Llc Methods and means to modify a plant genome
EP2822962A1 (de) 2012-03-08 2015-01-14 Athenix Corp. Axmi-345-delta-endotoxingen und verfahren zu dessen verwendung
WO2013134523A1 (en) 2012-03-08 2013-09-12 Athenix Corp. Bacillus thuringiensis toxin gene axmi335 and methods for its use
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
US9567381B2 (en) 2012-03-09 2017-02-14 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
CA2877290A1 (en) 2012-06-19 2013-12-27 Daniel F. Voytas Gene targeting in plants using dna viruses
CA2840683C (en) 2012-06-29 2020-03-10 Athenix Corp. Axmi277 nematode toxin and methods for its use
US9713687B2 (en) 2012-08-21 2017-07-25 Philip Morris Usa Inc. Ventilator aerosol delivery system with transition adapter for introducing carrier gas
US9758793B2 (en) 2012-08-30 2017-09-12 Athenix Corp. AXMI-234 and AXMI-235 delta-endotoxin genes and methods for their use
TWI670004B (zh) 2012-09-07 2019-09-01 美商陶氏農業科學公司 用來產生植物之螢光激活細胞分選富增技術
HUE039836T2 (hu) 2013-02-13 2019-02-28 Athenix Corp AXMI184 alkalmazása gyökérféreg rovarok kontrollálására
MX369500B (es) 2013-03-07 2019-11-11 Athenix Corp Genes de toxinas y métodos para su uso.
CA2903226C (en) 2013-03-15 2022-03-15 Bayer Cropscience Lp Constitutive soybean promoters
AU2014279694B2 (en) 2013-06-14 2020-07-23 Cellectis Methods for non-transgenic genome editing in plants
UY35696A (es) 2013-08-09 2015-03-27 Athenix Corp ?molécula de adn recombinante que comprende al gen de toxina axmi440, vector, célula huésped, plantas, composiciones y métodos relacionados?.
WO2015038262A2 (en) 2013-08-09 2015-03-19 Athenix Corp. Axmi281 toxin gene and methods for its use
WO2015077525A1 (en) 2013-11-25 2015-05-28 Bayer Cropscience Lp Use of axmi-011 for the control of hemipteran insects
UA122475C2 (uk) 2013-12-09 2020-11-10 Атенікс Корп. Ген токсину ахмі486 та спосіб його застосування
WO2016100832A1 (en) 2014-12-19 2016-06-23 AgBiome, Inc. Sequences to facilitate incorporation of dna into the genome of an organism
RU2020121273A (ru) 2014-12-22 2020-11-03 Агбайоми, Инк. Пестицидные гены и способы их применения
EP3283504A1 (de) 2015-04-17 2018-02-21 Agbiome, Inc. Pestizidgene und verfahren zur verwendung
CA2981088A1 (en) 2015-04-22 2016-10-27 AgBiome, Inc. Pesticidal genes and methods of use
EP3960864A3 (de) 2015-06-03 2022-06-15 Agbiome, Inc. Pestizidgene und verfahren zur verwendung
WO2016209708A1 (en) 2015-06-22 2016-12-29 AgBiome, Inc. Pesticidal genes and methods of use
CA3222614A1 (en) 2015-12-22 2017-06-29 AgBiome, Inc. Pesticidal genes and methods of use
US11624076B2 (en) 2016-04-21 2023-04-11 BASF Agricultural Solutions Seed US LLC TAL-effector mediated herbicide tolerance
WO2017205665A1 (en) 2016-05-25 2017-11-30 Cargill, Incorporated Engineered nucleases to generate deletion mutants in plants
WO2018048915A1 (en) 2016-09-06 2018-03-15 AgBiome, Inc. Pesticidal genes and methods of use
TW201815278A (zh) * 2016-10-20 2018-05-01 美商陶氏農業科學公司 加速生產胚癒合組織、體胚以及相關轉形方法
CN110267527A (zh) 2016-10-21 2019-09-20 韦斯塔隆公司 可切割肽及包含可切割肽的杀昆虫和杀线虫蛋白
AU2017365169B2 (en) 2016-11-23 2022-07-21 BASF Agricultural Solutions Seed US LLC Axmi669 and Axmi991 toxin genes and methods for their use
AU2017382305A1 (en) 2016-12-22 2019-07-18 BASF Agricultural Solutions Seed US LLC Use of CRY14 for the control of nematode pests
EP3571303A1 (de) 2017-01-18 2019-11-27 Basf Agricultural Solutions Seed Us Llc Bp005-toxin-gen und verfahren zu dessen verwendung
WO2018136611A1 (en) 2017-01-18 2018-07-26 Bayer Cropscience Lp Use of bp005 for the control of plant pathogens
BR112019015582A2 (pt) 2017-01-30 2020-03-10 AgBiome, Inc. Genes pesticidas e métodos de uso
BR112019021380A2 (pt) 2017-04-11 2020-05-05 Agbiome Inc genes pesticidas e métodos de uso
WO2018234191A1 (en) 2017-06-19 2018-12-27 University Of Copenhagen INCREASED STRENGTH IN DROUGHT IN PLANTS
CA3071862A1 (en) 2017-08-03 2019-02-07 AgBiome, Inc. Pesticidal genes and methods of use
AR113761A1 (es) 2017-10-18 2020-06-10 Syngenta Participations Ag Control de plagas de hemípteros utilizando moléculas de arn
CN108080043B (zh) * 2017-11-29 2019-07-19 华中科技大学 真空负压进样的多通道微流控芯片装置及制备方法与应用
WO2019126479A1 (en) 2017-12-22 2019-06-27 AgBiome, Inc. Pesticidal genes and methods of use
EP3850000A1 (de) 2018-09-14 2021-07-21 Vestaron Corporation Insektizide av3-mutant-polypeptide und verfahren zur herstellung und verwendung davon
WO2020142598A2 (en) 2019-01-04 2020-07-09 Cargill, Incorporated Engineered nucleases to generate mutations in plants
WO2021058659A1 (en) 2019-09-26 2021-04-01 Bayer Aktiengesellschaft Rnai-mediated pest control
JP2023522947A (ja) 2020-04-20 2023-06-01 ヴェスタロン・コーポレーション タンパク質分解に対して安定な害虫制御用ｕ１－アガトキシン－ｔａ１ｂバリアントポリペプチド
IL297738A (en) 2020-05-01 2022-12-01 Vestaron Corp Insecticide combinations
CN111778162A (zh) * 2020-07-29 2020-10-16 湖北明德健康科技有限公司 一种干细胞培养灌流装置
WO2022067214A2 (en) 2020-09-28 2022-03-31 Vestaron Corporation Mu-diguetoxin-dc1a variant polypeptides for pest control
WO2022115524A2 (en) 2020-11-24 2022-06-02 AgBiome, Inc. Pesticidal genes and methods of use
AU2022249371A1 (en) 2021-04-01 2023-10-26 Vestaron Corporation Av3 mutant polypeptides for pest control
AR125794A1 (es) 2021-05-06 2023-08-16 Agbiome Inc Genes plaguicidas y métodos de uso
WO2023107943A1 (en) 2021-12-07 2023-06-15 AgBiome, Inc. Pesticidal genes and methods of use
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides
WO2024044596A1 (en) 2022-08-23 2024-02-29 AgBiome, Inc. Pesticidal genes and methods of use

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3854321A (en) 1973-04-27 1974-12-17 B Dahneke Aerosol beam device and method
US5141131A (en) * 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
US5240842A (en) 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
AU642889B2 (en) 1989-07-11 1993-11-04 Biotechnology Research And Development Corporation Aerosol beam microinjector
ATE130371T1 (de) 1989-12-19 1995-12-15 Ciba Geigy Ag Verfahren und vorrichtung zur genetischen transformation von zellen.
US5212365A (en) * 1991-12-27 1993-05-18 Cetac Technologies, Inc. Direct injection micro nebulizer system and method of use
US5247842A (en) * 1991-09-30 1993-09-28 Tsi Incorporated Electrospray apparatus for producing uniform submicrometer droplets
FR2711523B1 (fr) * 1993-10-26 1996-02-16 Transgene Sa Procédé de préparation d'un aérosol viral.
US5455240A (en) * 1994-06-20 1995-10-03 The Rockefeller University Modulators of pneumococcal adhesion to cellular targets involving the platelet activating factor receptor, and uses thereof
US6576455B1 (en) 1995-04-20 2003-06-10 Basf Corporation Structure-based designed herbicide resistant products
US5770272A (en) * 1995-04-28 1998-06-23 Massachusetts Institute Of Technology Matrix-bearing targets for maldi mass spectrometry and methods of production thereof
US5868322A (en) * 1996-01-31 1999-02-09 Hewlett-Packard Company Apparatus for forming liquid droplets having a mechanically fixed inner microtube
DE69712754T2 (de) * 1996-03-01 2002-10-17 Univ California Hemmung der selectinbindung
US6433154B1 (en) * 1997-06-12 2002-08-13 Bristol-Myers Squibb Company Functional receptor/kinase chimera in yeast cells
US6946295B2 (en) * 1998-06-12 2005-09-20 Cellfor, Inc. Process for ex vitro sowing and germination of plant somatic embryos
EP1250447B1 (de) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Verfahren, Medien und Vorrichtung zur Einführung von Molekülen in Pflanzenzellen und Bakterien mittels Aerosolstrahlen
AU3934302A (en) 2000-11-28 2002-06-11 Midwest Oilseeds Inc Methods and compositions for the introduction of molecules into cells

Also Published As

Publication number Publication date
US6809232B1 (en) 2004-10-26
WO2001038514A2 (en) 2001-05-31
US7026286B2 (en) 2006-04-11
US7638115B2 (en) 2009-12-29
US20040259733A1 (en) 2004-12-23
US20060051870A1 (en) 2006-03-09
EP1250447A2 (de) 2002-10-23
BR0016030A (pt) 2002-07-30
AU1803601A (en) 2001-06-04
US7001754B2 (en) 2006-02-21
US20010026941A1 (en) 2001-10-04
CA2396392A1 (en) 2001-05-31
US20040259830A1 (en) 2004-12-23
AR026645A1 (es) 2003-02-19
EP1250447B1 (de) 2011-12-21
CA2396392C (en) 2015-04-21
US20040259831A1 (en) 2004-12-23
US7067716B2 (en) 2006-06-27
WO2001038514A3 (en) 2002-01-31

Similar Documents

Publication Publication Date Title
ATE538205T1 (de) Verfahren, medien und vorrichtung zur einführung von molekülen in pflanzenzellen und bakterien mittels aerosolstrahlen
DE10084820T1 (de) Verfahren und Vorrichtung zur Reinigung von sauerstoffhaltigem Gas
DE50000534D1 (de) Verfahren und vorrichtung zur durchführung chemischer und physikalischer prozesse
DE60000307T2 (de) Verfahren und Vorrichtung zur Feststellung von Service Abweichungen in transaktionsorientierten Netzwerken
DE69938068D1 (de) Vorrichtung zur Zerstäubung von Flüssigkeiten und Verfahren zum Schneiden
DE60026253D1 (de) Verfahren und Vorrichtung zur Verschlüsselung eines Dateninhalts
DE60021216D1 (de) Vorrichtung zur Wiedergabe von Musik, Verfahren zur Taktierungsanalyse und Speichermedium
DE69506549T2 (de) Vorrichtung und verfahren zur abgabe von chemischen lösungen
ATE110415T1 (de) Verbessertes verfahren und apparat, um biologische substanzen in lebenden zellen einzuführen.
DE60018733D1 (de) Vorrichtung und verfahren zur probenanalyse
DE60021077D1 (de) Vorrichtung und verfahren zur probenabgabe
DE60113021D1 (de) Verfahren und Vorrichtung zum Sammeln von Edelgas
DE60122612D1 (de) System, Verfahren und Vorrichtung zur Authentifizierung
DE60045910D1 (de) Verfahren und Vorrichtung zur Analyse von Teilchenmaterial in Gas.
DE60013790D1 (de) Verfahren und vorrichtung zur behandlung eines wässrigen mediums
DE60019169D1 (de) Verfahren, katalysatorsystem und vorrichtung zur behandlung von schwefelhaltigem gas
DE60006241D1 (de) Verfahren und vorrichtung zur abgabe von viskosem material
DE60005311D1 (de) Verfahren und vorrichtung zur minimalisierung differentieller stromverbrauchsangriffe
DE60225612D1 (de) Verfahren und Vorrichtung zum Erfrischen von Referenzzellen
DE69939083D1 (de) Elektrooptisches Verfahren zur Signalverarbeitung, Vorrichtung zur Durchführung des Verfahrens und Anwendung
DE59901114D1 (de) Verfahren und vorrichtung zum verdampfen von flüssigem sauerstoff
DE60124780D1 (de) Vorrichtung und verfahren zur serienvermehrung von säugerzellen zum animpfen
AT4855U3 (de) Verfahren und vorrichtung zum behandeln von maische
DE60130970D1 (de) Reagenziensatz zur detektion von mikroorganismen, vorrichtung zur quantifizierungvon mikroorganismen und verfahren zur quantifizierung von mikroorganismen
DE69719892D1 (de) Material, Verfahren und Vorrichtung zur Hemmung des Bakterienwachstums in wässrigen Medien