ATE474454T1 - Verfahren zur einbringung von molekülen in zellen - Google Patents
Verfahren zur einbringung von molekülen in zellenInfo
- Publication number
- ATE474454T1 ATE474454T1 AT01987094T AT01987094T ATE474454T1 AT E474454 T1 ATE474454 T1 AT E474454T1 AT 01987094 T AT01987094 T AT 01987094T AT 01987094 T AT01987094 T AT 01987094T AT E474454 T1 ATE474454 T1 AT E474454T1
- Authority
- AT
- Austria
- Prior art keywords
- cells
- directed
- present
- introducing molecules
- molecules
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
- C12N15/895—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Electromagnetism (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/724,265 US6809232B1 (en) | 1999-11-29 | 2000-11-28 | Methods and compositions for the introduction of molecules into cells |
PCT/US2001/044190 WO2002044391A2 (en) | 2000-11-28 | 2001-11-28 | Methods and compositions for the introduction of molecules into cells |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE474454T1 true ATE474454T1 (de) | 2010-08-15 |
Family
ID=24909723
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT01987094T ATE474454T1 (de) | 2000-11-28 | 2001-11-28 | Verfahren zur einbringung von molekülen in zellen |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1337140B1 (de) |
AT (1) | ATE474454T1 (de) |
AU (2) | AU2002239343B2 (de) |
CA (1) | CA2429272C (de) |
DE (1) | DE60142628D1 (de) |
WO (1) | WO2002044391A2 (de) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7314969B2 (en) | 1999-11-29 | 2008-01-01 | Midwest Oilseeds, Inc. | Methods and compositions for the introduction of molecules into cells |
EP1250447B1 (de) | 1999-11-29 | 2011-12-21 | Midwest Oilseeds, Inc. | Verfahren, Medien und Vorrichtung zur Einführung von Molekülen in Pflanzenzellen und Bakterien mittels Aerosolstrahlen |
EP1444999B1 (de) | 2003-02-03 | 2010-07-21 | BioWare Technology Co., Ltd | Genpistole mit Niederdruckgasbeschleunigung |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3854321A (en) * | 1973-04-27 | 1974-12-17 | B Dahneke | Aerosol beam device and method |
WO1991000915A1 (en) * | 1989-07-11 | 1991-01-24 | Biotechnology Research & Development Corporation | Aerosol beam microinjector |
US5240842A (en) * | 1989-07-11 | 1993-08-31 | Biotechnology Research And Development Corporation | Aerosol beam microinjector |
EP0434616B1 (de) * | 1989-12-19 | 1995-11-15 | Ciba-Geigy Ag | Verfahren und Vorrichtung zur genetischen Transformation von Zellen |
US6433154B1 (en) * | 1997-06-12 | 2002-08-13 | Bristol-Myers Squibb Company | Functional receptor/kinase chimera in yeast cells |
EP1250447B1 (de) * | 1999-11-29 | 2011-12-21 | Midwest Oilseeds, Inc. | Verfahren, Medien und Vorrichtung zur Einführung von Molekülen in Pflanzenzellen und Bakterien mittels Aerosolstrahlen |
-
2001
- 2001-11-28 AT AT01987094T patent/ATE474454T1/de not_active IP Right Cessation
- 2001-11-28 AU AU2002239343A patent/AU2002239343B2/en not_active Expired
- 2001-11-28 EP EP01987094A patent/EP1337140B1/de not_active Expired - Lifetime
- 2001-11-28 CA CA2429272A patent/CA2429272C/en not_active Expired - Lifetime
- 2001-11-28 DE DE60142628T patent/DE60142628D1/de not_active Expired - Lifetime
- 2001-11-28 AU AU3934302A patent/AU3934302A/xx active Pending
- 2001-11-28 WO PCT/US2001/044190 patent/WO2002044391A2/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CA2429272C (en) | 2016-06-28 |
WO2002044391A3 (en) | 2003-01-23 |
WO2002044391A2 (en) | 2002-06-06 |
AU2002239343B2 (en) | 2007-10-04 |
EP1337140B1 (de) | 2010-07-21 |
DE60142628D1 (de) | 2010-09-02 |
CA2429272A1 (en) | 2002-06-06 |
AU3934302A (en) | 2002-06-11 |
EP1337140A2 (de) | 2003-08-27 |
WO2002044391A9 (en) | 2003-05-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |