DE69829976D1 - Verfahren zur trocknung von kompetenten zellen - Google Patents

Verfahren zur trocknung von kompetenten zellen

Info

Publication number
DE69829976D1
DE69829976D1 DE69829976T DE69829976T DE69829976D1 DE 69829976 D1 DE69829976 D1 DE 69829976D1 DE 69829976 T DE69829976 T DE 69829976T DE 69829976 T DE69829976 T DE 69829976T DE 69829976 D1 DE69829976 D1 DE 69829976D1
Authority
DE
Germany
Prior art keywords
cells
competent
drying
competent cells
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69829976T
Other languages
English (en)
Other versions
DE69829976T2 (de
Inventor
A Jessee
R Bloom
Thuan Trinh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of DE69829976D1 publication Critical patent/DE69829976D1/de
Publication of DE69829976T2 publication Critical patent/DE69829976T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69829976T 1997-02-12 1998-02-11 Verfahren zur herstellung von zellen Expired - Fee Related DE69829976T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3972097P 1997-02-12 1997-02-12
US39720P 1997-02-12
PCT/US1998/002880 WO1998035018A1 (en) 1997-02-12 1998-02-11 Methods for lyophilizing competent cells

Publications (2)

Publication Number Publication Date
DE69829976D1 true DE69829976D1 (de) 2005-06-02
DE69829976T2 DE69829976T2 (de) 2006-04-27

Family

ID=21907025

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69829976T Expired - Fee Related DE69829976T2 (de) 1997-02-12 1998-02-11 Verfahren zur herstellung von zellen

Country Status (8)

Country Link
US (3) US6960464B2 (de)
EP (1) EP1005529B1 (de)
JP (1) JP2001512310A (de)
AT (1) ATE294229T1 (de)
DE (1) DE69829976T2 (de)
DK (1) DK1005529T3 (de)
ES (1) ES2241120T3 (de)
WO (1) WO1998035018A1 (de)

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US20040214306A1 (en) * 2003-01-23 2004-10-28 Bloom Fredric R. Rapid growing microorganisms for biotechnology applications
WO2004085613A2 (en) * 2003-03-19 2004-10-07 Stratagene Electrocompetent host cells
TWI414602B (zh) 2010-12-14 2013-11-11 Ind Tech Res Inst 新穎之大腸桿菌與其用途以及製備勝任細胞的方法
CN103387946A (zh) * 2012-05-11 2013-11-13 中国人民解放军军事医学科学院放射与辐射医学研究所 一种感受态宿主细胞的制备方法以及一种快速高效将外源物质转入宿主细胞的方法及其应用
US20150267210A1 (en) * 2012-10-16 2015-09-24 Genebridges Gmbh Electrocompetent cells and preparation thereof
CN105637097A (zh) 2013-08-05 2016-06-01 特韦斯特生物科学公司 从头合成的基因文库
US9752829B2 (en) * 2014-01-07 2017-09-05 Sudhir R. Brahmbhatt Liquid nitrogen (LIN) integrated lyophilization system for minimizing a carbon footprint
US9963672B2 (en) 2014-07-21 2018-05-08 New England Biolabs, Inc. Compositions and methods for extending storage time of competent cells at -20° C
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
JP6982362B2 (ja) 2015-09-18 2021-12-17 ツイスト バイオサイエンス コーポレーション オリゴ核酸変異体ライブラリーとその合成
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
WO2017095958A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
EP3500672A4 (de) 2016-08-22 2020-05-20 Twist Bioscience Corporation De-novo-synthetisierte nukleinsäure-bibliotheken
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10463065B2 (en) * 2016-12-01 2019-11-05 Council Of Scientific And Industrial Research Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni
EA201991262A1 (ru) 2016-12-16 2020-04-07 Твист Байосайенс Корпорейшн Библиотеки вариантов иммунологического синапса и их синтез
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566209A (zh) 2017-06-12 2020-08-21 特韦斯特生物科学公司 无缝核酸装配方法
KR20200047706A (ko) 2017-09-11 2020-05-07 트위스트 바이오사이언스 코포레이션 Gpcr 결합 단백질 및 이의 합성 방법
CA3079613A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
JP7191448B2 (ja) 2018-01-04 2022-12-19 ツイスト バイオサイエンス コーポレーション Ｄｎａベースのデジタル情報ストレージ
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
EP3938506A4 (de) 2019-02-26 2022-12-14 Twist Bioscience Corporation Variante nukleinsäurebibliotheken zur optimierung von antikörpern
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
CN113817609A (zh) * 2021-11-01 2021-12-21 苏州科正环保科技有限公司 基因工程菌的冻干保藏方法

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DE3515586A1 (de) 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes sarcosinoxidase-praeparat
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FR2625221B1 (fr) 1987-12-24 1990-06-15 Sanofi Sa Protoplastes stabilises et procede pour leur obtention
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Also Published As

Publication number Publication date
US20020164771A1 (en) 2002-11-07
DK1005529T3 (da) 2005-06-13
US6960464B2 (en) 2005-11-01
ES2241120T3 (es) 2005-10-16
DE69829976T2 (de) 2006-04-27
JP2001512310A (ja) 2001-08-21
WO1998035018A1 (en) 1998-08-13
ATE294229T1 (de) 2005-05-15
US20060014255A1 (en) 2006-01-19
EP1005529A1 (de) 2000-06-07
EP1005529B1 (de) 2005-04-27
US7648832B2 (en) 2010-01-19
US20100081171A1 (en) 2010-04-01
EP1005529A4 (de) 2002-06-12

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee