DK1005529T3 - Fremgangsmåder til lyofilisering af kompetente celler - Google Patents

Fremgangsmåder til lyofilisering af kompetente celler

Info

Publication number
DK1005529T3
DK1005529T3 DK98906440T DK98906440T DK1005529T3 DK 1005529 T3 DK1005529 T3 DK 1005529T3 DK 98906440 T DK98906440 T DK 98906440T DK 98906440 T DK98906440 T DK 98906440T DK 1005529 T3 DK1005529 T3 DK 1005529T3
Authority
DK
Denmark
Prior art keywords
cells
competent
methods
lyophilization
competent cells
Prior art date
Application number
DK98906440T
Other languages
English (en)
Inventor
Joel A Jessee
Frederic R Bloom
Thuan Trinh
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of DK1005529T3 publication Critical patent/DK1005529T3/da

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK98906440T 1997-02-12 1998-02-11 Fremgangsmåder til lyofilisering af kompetente celler DK1005529T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3972097P 1997-02-12 1997-02-12
PCT/US1998/002880 WO1998035018A1 (en) 1997-02-12 1998-02-11 Methods for lyophilizing competent cells

Publications (1)

Publication Number Publication Date
DK1005529T3 true DK1005529T3 (da) 2005-06-13

Family

ID=21907025

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98906440T DK1005529T3 (da) 1997-02-12 1998-02-11 Fremgangsmåder til lyofilisering af kompetente celler

Country Status (8)

Country Link
US (3) US6960464B2 (da)
EP (1) EP1005529B1 (da)
JP (1) JP2001512310A (da)
AT (1) ATE294229T1 (da)
DE (1) DE69829976T2 (da)
DK (1) DK1005529T3 (da)
ES (1) ES2241120T3 (da)
WO (1) WO1998035018A1 (da)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2241120T3 (es) * 1997-02-12 2005-10-16 Invitrogen Corporation Metodos para liofilizar celulas competentes.
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
US20020081565A1 (en) * 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
US20040110267A1 (en) * 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
WO2002061111A2 (en) * 2000-12-15 2002-08-08 Stratagene Room temperature stable competent cells
WO2004065568A2 (en) * 2003-01-23 2004-08-05 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
WO2004085613A2 (en) * 2003-03-19 2004-10-07 Stratagene Electrocompetent host cells
TWI414602B (zh) 2010-12-14 2013-11-11 Ind Tech Res Inst 新穎之大腸桿菌與其用途以及製備勝任細胞的方法
CN103387946A (zh) * 2012-05-11 2013-11-13 中国人民解放军军事医学科学院放射与辐射医学研究所 一种感受态宿主细胞的制备方法以及一种快速高效将外源物质转入宿主细胞的方法及其应用
GB2520903A (en) 2012-10-16 2015-06-03 Gene Bridges Gmbh Electrocompetent cells and preparation thereof
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
US9752829B2 (en) * 2014-01-07 2017-09-05 Sudhir R. Brahmbhatt Liquid nitrogen (LIN) integrated lyophilization system for minimizing a carbon footprint
US9963672B2 (en) 2014-07-21 2018-05-08 New England Biolabs, Inc. Compositions and methods for extending storage time of competent cells at -20° C
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation BANKS OF OLIGONUCLEIC ACID VARIANTS AND SYNTHESIS THEREOF
KR20180058772A (ko) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
CA3034769A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10463065B2 (en) * 2016-12-01 2019-11-05 Council Of Scientific And Industrial Research Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
AU2018284227B2 (en) 2017-06-12 2024-05-02 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
GB2583590A (en) 2017-10-20 2020-11-04 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
AU2019205269A1 (en) 2018-01-04 2020-07-30 Twist Bioscience Corporation DNA-based digital information storage
CN112639130B (zh) 2018-05-18 2024-08-09 特韦斯特生物科学公司 用于核酸杂交的多核苷酸、试剂和方法
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
AU2020356471A1 (en) 2019-09-23 2022-04-21 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CN113817609A (zh) * 2021-11-01 2021-12-21 苏州科正环保科技有限公司 基因工程菌的冻干保藏方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3843453A (en) 1970-11-27 1974-10-22 Miles Lab Process and composition for use in microbiological quality assurance
US4038143A (en) 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
JPS57170184A (en) 1980-07-18 1982-10-20 Unisearch Ltd Mutant of e. coli and production of phenylalanine
US4446230A (en) 1981-10-30 1984-05-01 Temple University Of The Commonwealth System Of Higher Education Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae
US4520019A (en) 1982-04-29 1985-05-28 Ribi Immunochem Research, Inc. Stable composition and preparation thereof
JPS60258125A (ja) 1984-06-06 1985-12-20 Hayashibara Biochem Lab Inc 蛋白性生理活性物質を含有する水溶性乾燥物
DE3515586A1 (de) 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes sarcosinoxidase-praeparat
US4891319A (en) 1985-07-09 1990-01-02 Quadrant Bioresources Limited Protection of proteins and the like
US4981797A (en) 1985-08-08 1991-01-01 Life Technologies, Inc. Process of producing highly transformable cells and cells produced thereby
US4851348A (en) 1986-07-16 1989-07-25 Cold Spring Harbor Laboratory Biologically pure escherichia coli cell line which is a deoR- mutant and which is more transformation efficient with foreign plasmids than deoR+ escherichia coli cell lines, processes for obtaining these cell lines, methods of use
US5292507A (en) 1986-08-01 1994-03-08 Imperial Oil Limited Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
FR2625221B1 (fr) 1987-12-24 1990-06-15 Sanofi Sa Protoplastes stabilises et procede pour leur obtention
US4808404A (en) 1988-01-11 1989-02-28 A. H. Robins Company, Inc. Live vaccine for coccidiosis utilizing coccidial sporozoites
US5043261A (en) 1989-04-10 1991-08-27 Cryopharm Corporation Lyophilized and reconstituted red blood cell and hemosome compositions
US5213814A (en) 1989-04-10 1993-05-25 Cryopharm Corporation Lyophilized and reconstituted blood platelet compositions
US5153004A (en) 1989-06-02 1992-10-06 Cryopharm Corporation Freezing and thawing of erythrocytes
US5178884A (en) 1988-05-18 1993-01-12 Cryopharm Corporation Lyophilized and reconstituted red blood cell compositions
US5045446A (en) 1988-08-26 1991-09-03 Cryopharm Corporation Lyophilization of cells
US5958670A (en) 1988-05-18 1999-09-28 Cobe Laboratories, Inc. Method of freezing cells and cell-like materials
US5059518A (en) 1988-10-20 1991-10-22 Coulter Corporation Stabilized lyophilized mammalian cells and method of making same
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
US5149656A (en) 1990-05-03 1992-09-22 University Of Florida Research Foundation, Inc. Microbiological assay pad and kit for selective detection of toxicants
US5200399A (en) * 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5733774A (en) * 1995-02-02 1998-03-31 Ecoscience Corporation Method and composition for producing stable bacteria and bacterial formulations
US6274369B1 (en) 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
EP0921814A4 (en) 1996-03-29 2001-10-04 Life Technologies Inc Process for increasing the viability and transformation efficiency of bacteria during storage at low temperatures
ES2241120T3 (es) 1997-02-12 2005-10-16 Invitrogen Corporation Metodos para liofilizar celulas competentes.
US6383810B2 (en) 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
US6610531B1 (en) * 1998-09-24 2003-08-26 The United States Of America As Represented By The Secretary Of The Navy Viable dried bacteria produced by drying in the presence of trehalose and divalent cation
US20030044965A1 (en) * 1998-09-24 2003-03-06 Alfred Mateczun Long term preservation and storage of viable dried bacteria
US20020081565A1 (en) * 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
US6872357B1 (en) * 2000-11-22 2005-03-29 Quadrant Drug Delivery Limited Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying
WO2002061111A2 (en) * 2000-12-15 2002-08-08 Stratagene Room temperature stable competent cells
WO2002061035A2 (en) * 2000-12-15 2002-08-08 Stratagene Mutant cells with enhanced resistance to desiccation
US20040110267A1 (en) * 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
WO2002090513A2 (en) * 2001-05-07 2002-11-14 Universal Preservation Technologies, Inc. Preservation of competent cells at ambient temperatures

Also Published As

Publication number Publication date
ES2241120T3 (es) 2005-10-16
US20020164771A1 (en) 2002-11-07
EP1005529A4 (en) 2002-06-12
JP2001512310A (ja) 2001-08-21
DE69829976T2 (de) 2006-04-27
WO1998035018A1 (en) 1998-08-13
EP1005529B1 (en) 2005-04-27
US6960464B2 (en) 2005-11-01
US7648832B2 (en) 2010-01-19
US20100081171A1 (en) 2010-04-01
DE69829976D1 (de) 2005-06-02
US20060014255A1 (en) 2006-01-19
EP1005529A1 (en) 2000-06-07
ATE294229T1 (de) 2005-05-15

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