ATE294229T1 - Verfahren zur trocknung von kompetenten zellen - Google Patents

Verfahren zur trocknung von kompetenten zellen

Info

Publication number
ATE294229T1
ATE294229T1 AT98906440T AT98906440T ATE294229T1 AT E294229 T1 ATE294229 T1 AT E294229T1 AT 98906440 T AT98906440 T AT 98906440T AT 98906440 T AT98906440 T AT 98906440T AT E294229 T1 ATE294229 T1 AT E294229T1
Authority
AT
Austria
Prior art keywords
cells
competent
drying
competent cells
producing
Prior art date
Application number
AT98906440T
Other languages
English (en)
Inventor
Joel A Jessee
Frederic R Bloom
Thuan Trinh
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE294229T1 publication Critical patent/ATE294229T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT98906440T 1997-02-12 1998-02-11 Verfahren zur trocknung von kompetenten zellen ATE294229T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3972097P 1997-02-12 1997-02-12
PCT/US1998/002880 WO1998035018A1 (en) 1997-02-12 1998-02-11 Methods for lyophilizing competent cells

Publications (1)

Publication Number Publication Date
ATE294229T1 true ATE294229T1 (de) 2005-05-15

Family

ID=21907025

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98906440T ATE294229T1 (de) 1997-02-12 1998-02-11 Verfahren zur trocknung von kompetenten zellen

Country Status (8)

Country Link
US (3) US6960464B2 (de)
EP (1) EP1005529B1 (de)
JP (1) JP2001512310A (de)
AT (1) ATE294229T1 (de)
DE (1) DE69829976T2 (de)
DK (1) DK1005529T3 (de)
ES (1) ES2241120T3 (de)
WO (1) WO1998035018A1 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE294229T1 (de) * 1997-02-12 2005-05-15 Invitrogen Corp Verfahren zur trocknung von kompetenten zellen
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
US20020081565A1 (en) * 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
WO2002061111A2 (en) * 2000-12-15 2002-08-08 Stratagene Room temperature stable competent cells
US20040110267A1 (en) * 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
US20040214306A1 (en) * 2003-01-23 2004-10-28 Bloom Fredric R. Rapid growing microorganisms for biotechnology applications
WO2004085613A2 (en) * 2003-03-19 2004-10-07 Stratagene Electrocompetent host cells
TWI414602B (zh) 2010-12-14 2013-11-11 Ind Tech Res Inst 新穎之大腸桿菌與其用途以及製備勝任細胞的方法
CN103387946A (zh) * 2012-05-11 2013-11-13 中国人民解放军军事医学科学院放射与辐射医学研究所 一种感受态宿主细胞的制备方法以及一种快速高效将外源物质转入宿主细胞的方法及其应用
US20150267210A1 (en) * 2012-10-16 2015-09-24 Genebridges Gmbh Electrocompetent cells and preparation thereof
DK3030682T3 (da) 2013-08-05 2020-09-14 Twist Bioscience Corp De novo synthesized gene libraries
US9752829B2 (en) * 2014-01-07 2017-09-05 Sudhir R. Brahmbhatt Liquid nitrogen (LIN) integrated lyophilization system for minimizing a carbon footprint
WO2016014307A1 (en) * 2014-07-21 2016-01-28 New England Biolabs, Inc. Compositions and methods for extending storage time of competent cells at -20 c
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2017049231A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
US9895673B2 (en) 2015-12-01 2018-02-20 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
EP3500672A4 (de) 2016-08-22 2020-05-20 Twist Bioscience Corporation De-novo-synthetisierte nukleinsäure-bibliotheken
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10463065B2 (en) * 2016-12-01 2019-11-05 Council Of Scientific And Industrial Research Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni
WO2018112426A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CN118116478A (zh) 2017-02-22 2024-05-31 特韦斯特生物科学公司 基于核酸的数据存储
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
GB2578844A (en) 2017-06-12 2020-05-27 Twist Bioscience Corp Methods for seamless nucleic acid assembly
WO2019051501A1 (en) 2017-09-11 2019-03-14 Twist Bioscience Corporation PROTEINS BINDING TO GPCR AND METHODS OF SYNTHESIS
GB2583590A (en) 2017-10-20 2020-11-04 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
AU2019205269A1 (en) 2018-01-04 2020-07-30 Twist Bioscience Corporation DNA-based digital information storage
GB2590196A (en) 2018-05-18 2021-06-23 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
SG11202109322TA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for glp1 receptor
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
JP2022548309A (ja) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション Ｃｒｔｈ２のバリアント核酸ライブラリー
CN113817609A (zh) * 2021-11-01 2021-12-21 苏州科正环保科技有限公司 基因工程菌的冻干保藏方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3843453A (en) 1970-11-27 1974-10-22 Miles Lab Process and composition for use in microbiological quality assurance
US4038143A (en) 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
JPS57170184A (en) 1980-07-18 1982-10-20 Unisearch Ltd Mutant of e. coli and production of phenylalanine
US4446230A (en) 1981-10-30 1984-05-01 Temple University Of The Commonwealth System Of Higher Education Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae
US4520019A (en) 1982-04-29 1985-05-28 Ribi Immunochem Research, Inc. Stable composition and preparation thereof
JPS60258125A (ja) 1984-06-06 1985-12-20 Hayashibara Biochem Lab Inc 蛋白性生理活性物質を含有する水溶性乾燥物
DE3515586A1 (de) 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes sarcosinoxidase-praeparat
DE3682047D1 (de) 1985-07-09 1991-11-21 Quadrant Bioresources Ltd Beschuetzung von proteinen und aehnlichem.
US4981797A (en) 1985-08-08 1991-01-01 Life Technologies, Inc. Process of producing highly transformable cells and cells produced thereby
US4851348A (en) 1986-07-16 1989-07-25 Cold Spring Harbor Laboratory Biologically pure escherichia coli cell line which is a deoR- mutant and which is more transformation efficient with foreign plasmids than deoR+ escherichia coli cell lines, processes for obtaining these cell lines, methods of use
US5292507A (en) 1986-08-01 1994-03-08 Imperial Oil Limited Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
FR2625221B1 (fr) 1987-12-24 1990-06-15 Sanofi Sa Protoplastes stabilises et procede pour leur obtention
US4808404A (en) 1988-01-11 1989-02-28 A. H. Robins Company, Inc. Live vaccine for coccidiosis utilizing coccidial sporozoites
US5043261A (en) 1989-04-10 1991-08-27 Cryopharm Corporation Lyophilized and reconstituted red blood cell and hemosome compositions
US5213814A (en) 1989-04-10 1993-05-25 Cryopharm Corporation Lyophilized and reconstituted blood platelet compositions
US5045446A (en) 1988-08-26 1991-09-03 Cryopharm Corporation Lyophilization of cells
US5153004A (en) 1989-06-02 1992-10-06 Cryopharm Corporation Freezing and thawing of erythrocytes
US5178884A (en) 1988-05-18 1993-01-12 Cryopharm Corporation Lyophilized and reconstituted red blood cell compositions
US5958670A (en) 1988-05-18 1999-09-28 Cobe Laboratories, Inc. Method of freezing cells and cell-like materials
US5059518A (en) 1988-10-20 1991-10-22 Coulter Corporation Stabilized lyophilized mammalian cells and method of making same
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
US5149656A (en) 1990-05-03 1992-09-22 University Of Florida Research Foundation, Inc. Microbiological assay pad and kit for selective detection of toxicants
US5200399A (en) * 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5733774A (en) * 1995-02-02 1998-03-31 Ecoscience Corporation Method and composition for producing stable bacteria and bacterial formulations
US6274369B1 (en) 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
WO1997036613A1 (en) 1996-03-29 1997-10-09 Life Technologies, Inc. Method for increasing viability and transformation efficiency of bacteria during storage at low temperatures
ATE294229T1 (de) 1997-02-12 2005-05-15 Invitrogen Corp Verfahren zur trocknung von kompetenten zellen
US6383810B2 (en) 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
US6610531B1 (en) * 1998-09-24 2003-08-26 The United States Of America As Represented By The Secretary Of The Navy Viable dried bacteria produced by drying in the presence of trehalose and divalent cation
US20030044965A1 (en) * 1998-09-24 2003-03-06 Alfred Mateczun Long term preservation and storage of viable dried bacteria
US20020081565A1 (en) * 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
US6872357B1 (en) * 2000-11-22 2005-03-29 Quadrant Drug Delivery Limited Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying
US20040110267A1 (en) * 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
WO2002061111A2 (en) * 2000-12-15 2002-08-08 Stratagene Room temperature stable competent cells
WO2002061035A2 (en) * 2000-12-15 2002-08-08 Stratagene Mutant cells with enhanced resistance to desiccation
AU2002305461A1 (en) * 2001-05-07 2002-11-18 Universal Preservation Technologies, Inc. Preservation of competent cells at ambient temperatures

Also Published As

Publication number Publication date
US20100081171A1 (en) 2010-04-01
EP1005529A4 (de) 2002-06-12
DE69829976T2 (de) 2006-04-27
US6960464B2 (en) 2005-11-01
US20020164771A1 (en) 2002-11-07
DE69829976D1 (de) 2005-06-02
EP1005529A1 (de) 2000-06-07
WO1998035018A1 (en) 1998-08-13
US20060014255A1 (en) 2006-01-19
DK1005529T3 (da) 2005-06-13
US7648832B2 (en) 2010-01-19
ES2241120T3 (es) 2005-10-16
JP2001512310A (ja) 2001-08-21
EP1005529B1 (de) 2005-04-27

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