ATE294229T1 - Verfahren zur trocknung von kompetenten zellen - Google Patents

Verfahren zur trocknung von kompetenten zellen

Info

Publication number
ATE294229T1
ATE294229T1 AT98906440T AT98906440T ATE294229T1 AT E294229 T1 ATE294229 T1 AT E294229T1 AT 98906440 T AT98906440 T AT 98906440T AT 98906440 T AT98906440 T AT 98906440T AT E294229 T1 ATE294229 T1 AT E294229T1
Authority
AT
Austria
Prior art keywords
cells
competent
drying
competent cells
producing
Prior art date
Application number
AT98906440T
Other languages
English (en)
Inventor
Joel A Jessee
Frederic R Bloom
Thuan Trinh
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE294229T1 publication Critical patent/ATE294229T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT98906440T 1997-02-12 1998-02-11 Verfahren zur trocknung von kompetenten zellen ATE294229T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3972097P 1997-02-12 1997-02-12
PCT/US1998/002880 WO1998035018A1 (en) 1997-02-12 1998-02-11 Methods for lyophilizing competent cells

Publications (1)

Publication Number Publication Date
ATE294229T1 true ATE294229T1 (de) 2005-05-15

Family

ID=21907025

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98906440T ATE294229T1 (de) 1997-02-12 1998-02-11 Verfahren zur trocknung von kompetenten zellen

Country Status (8)

Country Link
US (3) US6960464B2 (de)
EP (1) EP1005529B1 (de)
JP (1) JP2001512310A (de)
AT (1) ATE294229T1 (de)
DE (1) DE69829976T2 (de)
DK (1) DK1005529T3 (de)
ES (1) ES2241120T3 (de)
WO (1) WO1998035018A1 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE294229T1 (de) * 1997-02-12 2005-05-15 Invitrogen Corp Verfahren zur trocknung von kompetenten zellen
US6709852B1 (en) * 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
US20020081565A1 (en) * 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
US20040110267A1 (en) * 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
AU2002248192A1 (en) * 2000-12-15 2002-08-12 Stratagene Room temperature stable competent cells
US20040214306A1 (en) * 2003-01-23 2004-10-28 Bloom Fredric R. Rapid growing microorganisms for biotechnology applications
US20040209362A1 (en) * 2003-03-19 2004-10-21 Stratagene Electrocompetent host cells
TWI414602B (zh) 2010-12-14 2013-11-11 Ind Tech Res Inst 新穎之大腸桿菌與其用途以及製備勝任細胞的方法
CN103387946A (zh) * 2012-05-11 2013-11-13 中国人民解放军军事医学科学院放射与辐射医学研究所 一种感受态宿主细胞的制备方法以及一种快速高效将外源物质转入宿主细胞的方法及其应用
GB2520903A (en) * 2012-10-16 2015-06-03 Gene Bridges Gmbh Electrocompetent cells and preparation thereof
EP3030682B1 (de) 2013-08-05 2020-06-03 Twist Bioscience Corporation De-novo-synthetisierte genbanken
US9752829B2 (en) * 2014-01-07 2017-09-05 Sudhir R. Brahmbhatt Liquid nitrogen (LIN) integrated lyophilization system for minimizing a carbon footprint
US9963672B2 (en) 2014-07-21 2018-05-08 New England Biolabs, Inc. Compositions and methods for extending storage time of competent cells at -20° C
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
JP6982362B2 (ja) 2015-09-18 2021-12-17 ツイスト バイオサイエンス コーポレーション オリゴ核酸変異体ライブラリーとその合成
KR20250053972A (ko) 2015-09-22 2025-04-22 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
EP3384077A4 (de) 2015-12-01 2019-05-08 Twist Bioscience Corporation Funktionalisierte oberflächen und herstellung davon
EP3500672A4 (de) 2016-08-22 2020-05-20 Twist Bioscience Corporation De-novo-synthetisierte nukleinsäure-bibliotheken
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
US10463065B2 (en) * 2016-12-01 2019-11-05 Council Of Scientific And Industrial Research Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni
EP3554514A4 (de) 2016-12-16 2020-08-05 Twist Bioscience Corporation Variante bibliotheken der immunologischen synapse und synthese davon
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
SG11201912057RA (en) 2017-06-12 2020-01-30 Twist Bioscience Corp Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
JP2020536504A (ja) 2017-09-11 2020-12-17 ツイスト バイオサイエンス コーポレーション Ｇｐｃｒ結合タンパク質およびその合成
GB2583590A (en) 2017-10-20 2020-11-04 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
EP3728581B1 (de) * 2017-12-22 2024-10-09 Bio-Rad Laboratories, Inc. Steuerung des phänotyps von organismen mit crispr/cas-gen-targeting
GB2585506A (en) 2018-01-04 2021-01-13 Twist Bioscience Corp DNA-based digital information storage
CN118957038A (zh) 2018-05-18 2024-11-15 特韦斯特生物科学公司 用于核酸杂交的多核苷酸、试剂和方法
CN113692409B (zh) 2018-12-26 2025-01-10 特韦斯特生物科学公司 高度准确的从头多核苷酸合成
AU2020229349A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
EP4034566A4 (de) 2019-09-23 2024-01-24 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für crth2
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
CN113817609A (zh) * 2021-11-01 2021-12-21 苏州科正环保科技有限公司 基因工程菌的冻干保藏方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3843453A (en) 1970-11-27 1974-10-22 Miles Lab Process and composition for use in microbiological quality assurance
US4038143A (en) 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
JPS57170184A (en) 1980-07-18 1982-10-20 Unisearch Ltd Mutant of e. coli and production of phenylalanine
US4446230A (en) 1981-10-30 1984-05-01 Temple University Of The Commonwealth System Of Higher Education Test method for the laboratory diagnosis of Gonorrhea and test strain of neisseria gonorrhoeae
US4520019A (en) 1982-04-29 1985-05-28 Ribi Immunochem Research, Inc. Stable composition and preparation thereof
JPS60258125A (ja) 1984-06-06 1985-12-20 Hayashibara Biochem Lab Inc 蛋白性生理活性物質を含有する水溶性乾燥物
DE3515586A1 (de) 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes sarcosinoxidase-praeparat
EP0229810B1 (de) 1985-07-09 1991-10-16 Quadrant Bioresources Limited Beschützung von proteinen und ähnlichem
US4981797A (en) 1985-08-08 1991-01-01 Life Technologies, Inc. Process of producing highly transformable cells and cells produced thereby
US4851348A (en) 1986-07-16 1989-07-25 Cold Spring Harbor Laboratory Biologically pure escherichia coli cell line which is a deoR- mutant and which is more transformation efficient with foreign plasmids than deoR+ escherichia coli cell lines, processes for obtaining these cell lines, methods of use
US5292507A (en) 1986-08-01 1994-03-08 Imperial Oil Limited Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
FR2625221B1 (fr) 1987-12-24 1990-06-15 Sanofi Sa Protoplastes stabilises et procede pour leur obtention
US4808404A (en) 1988-01-11 1989-02-28 A. H. Robins Company, Inc. Live vaccine for coccidiosis utilizing coccidial sporozoites
US5153004A (en) 1989-06-02 1992-10-06 Cryopharm Corporation Freezing and thawing of erythrocytes
US5043261A (en) 1989-04-10 1991-08-27 Cryopharm Corporation Lyophilized and reconstituted red blood cell and hemosome compositions
US5958670A (en) 1988-05-18 1999-09-28 Cobe Laboratories, Inc. Method of freezing cells and cell-like materials
US5045446A (en) 1988-08-26 1991-09-03 Cryopharm Corporation Lyophilization of cells
US5178884A (en) 1988-05-18 1993-01-12 Cryopharm Corporation Lyophilized and reconstituted red blood cell compositions
US5213814A (en) 1989-04-10 1993-05-25 Cryopharm Corporation Lyophilized and reconstituted blood platelet compositions
US5059518A (en) 1988-10-20 1991-10-22 Coulter Corporation Stabilized lyophilized mammalian cells and method of making same
GB8903593D0 (en) 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
US5149656A (en) 1990-05-03 1992-09-22 University Of Florida Research Foundation, Inc. Microbiological assay pad and kit for selective detection of toxicants
US5200399A (en) 1990-09-14 1993-04-06 Boyce Thompson Institute For Plant Research, Inc. Method of protecting biological materials from destructive reactions in the dry state
US5733774A (en) 1995-02-02 1998-03-31 Ecoscience Corporation Method and composition for producing stable bacteria and bacterial formulations
US6274369B1 (en) 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
JP2001517928A (ja) 1996-03-29 2001-10-09 ライフ・テクノロジーズ・インコーポレイテッド 細菌の低温貯蔵中の生存能および形質転換効率増強法
ATE294229T1 (de) 1997-02-12 2005-05-15 Invitrogen Corp Verfahren zur trocknung von kompetenten zellen
US6383810B2 (en) 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
US6610531B1 (en) 1998-09-24 2003-08-26 The United States Of America As Represented By The Secretary Of The Navy Viable dried bacteria produced by drying in the presence of trehalose and divalent cation
US20030044965A1 (en) 1998-09-24 2003-03-06 Alfred Mateczun Long term preservation and storage of viable dried bacteria
US20020081565A1 (en) 2000-10-30 2002-06-27 Sigma-Aldrich Co. Process for producing freeze dried competent cells and use thereof in cloning
US6872357B1 (en) 2000-11-22 2005-03-29 Quadrant Drug Delivery Limited Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying
AU2002248178A1 (en) 2000-12-15 2002-08-12 Stratagene Mutant cells with enhanced resistance to desiccation
AU2002248192A1 (en) 2000-12-15 2002-08-12 Stratagene Room temperature stable competent cells
US20040110267A1 (en) 2000-12-15 2004-06-10 Stratagene Room temperature stable competent cells
AU2002305461A1 (en) 2001-05-07 2002-11-18 Universal Preservation Technologies, Inc. Preservation of competent cells at ambient temperatures

Also Published As

Publication number Publication date
US20060014255A1 (en) 2006-01-19
EP1005529A1 (de) 2000-06-07
EP1005529A4 (de) 2002-06-12
US7648832B2 (en) 2010-01-19
JP2001512310A (ja) 2001-08-21
DE69829976D1 (de) 2005-06-02
ES2241120T3 (es) 2005-10-16
EP1005529B1 (de) 2005-04-27
US20020164771A1 (en) 2002-11-07
US20100081171A1 (en) 2010-04-01
US6960464B2 (en) 2005-11-01
DK1005529T3 (da) 2005-06-13
WO1998035018A1 (en) 1998-08-13
DE69829976T2 (de) 2006-04-27

Similar Documents

Publication Publication Date Title
ATE294229T1 (de) Verfahren zur trocknung von kompetenten zellen
ATE309342T1 (de) Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen
ATE414162T1 (de) Verfahren und materialien zur herstellung von glucosamin
DE69738177D1 (de) Verbesserte nukleinsäuren, welche für eine chimäre glycosyltransferase kodieren
ATE213271T1 (de) Verfahren zur herstellung von rekombinanten proteinen in e. coli mittels hochzelldichte- fermentation
DE60333201D1 (de) Verfahren zur herstellung von proteinen
DK1042461T3 (da) Ekspressionskloning i filiforme svampe
FI925986A0 (fi) Slumpartat bio-oligomer bibliotek, ett foerfarande foer syntetisering avdet samt ett foerfarande foer anvaendning av det
DE69935978D1 (de) Kulturmedium zur kultivierung tierischer zellen und verfahren zur herstellung von proteinen durch dessen verwendung
ATE521695T1 (de) Zirkuläres dna-molekül mit einem konditionalen replikationsursprung, verfahren zur herstellung davon und ihre verwendung in der gentherapie
ATE278024T1 (de) Methode zur herstellung von rekombinanten zellen
DK1117798T3 (da) Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
ATE317899T1 (de) Gefrierschutzproteine aus choristoneura sp., gene und verfahren zu ihrer verwendung
ATE333502T1 (de) Verfahren zur herstellung von nukleinsäure- und polypeptidbanken durch in vivo rekombination und ihre verwendungen
DE69937056D1 (de) Verfahren zur transformation von tierzellen
ATE330003T1 (de) Rekombinanter stabiler zellklon, seine herstellung und verwendung
ATE258558T1 (de) Lantibiotikum verwandt mit actagardine, verfahren zur herstellung und verwendung desselben
ATE263239T1 (de) Produkte des keratinocyten-wachstumsfaktors 2 (kgf-2)
WO2004013333A3 (de) Revertierung der negativ-selektiven wirkung von negativen markerproteinen als selektionsverfahren
ATE329055T1 (de) Methode zur erstellung von genexpressionsprofilen
WO2002002591A3 (en) Peptides for use in culture media
ATE501272T1 (de) Verfahren zur produktion von pflanzen mit verbesserter transpirationseffizienz sowie davon produzierte pflanzen
EE9900447A (et) Raudasiduvat valku ületootev taimerakk, taim või taimeosa, raudasiduvat valku kodeeriva nukleotiidjärjestuse kasutamine, nukleiinhape ja meetod põllukultuuride saamiseks
HUP9801330A2 (hu) Új PKA-kötő fehérjék és alkalmazásuk
ATE269905T1 (de) Verfahren zur herstellung von proteinen in wirtszellen unter verwendung von chaperoninen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties