ATE309342T1 - Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen - Google Patents

Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen

Info

Publication number
ATE309342T1
ATE309342T1 AT99936725T AT99936725T ATE309342T1 AT E309342 T1 ATE309342 T1 AT E309342T1 AT 99936725 T AT99936725 T AT 99936725T AT 99936725 T AT99936725 T AT 99936725T AT E309342 T1 ATE309342 T1 AT E309342T1
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AT
Austria
Prior art keywords
sequences
library
recombined
sequence
fragments
Prior art date
Application number
AT99936725T
Other languages
English (en)
Inventor
Daniel Dupret
Jean-Michel Masson
Fabrice Lefevre
Original Assignee
Proteus Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus Sa filed Critical Proteus Sa
Application granted granted Critical
Publication of ATE309342T1 publication Critical patent/ATE309342T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
AT99936725T 1998-08-12 1999-08-11 Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen ATE309342T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9810338A FR2782323B1 (fr) 1998-08-12 1998-08-12 Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
PCT/FR1999/001973 WO2000009679A1 (fr) 1998-08-12 1999-08-11 Procede d'obtention in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues

Publications (1)

Publication Number Publication Date
ATE309342T1 true ATE309342T1 (de) 2005-11-15

Family

ID=9529621

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99936725T ATE309342T1 (de) 1998-08-12 1999-08-11 Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen

Country Status (12)

Country Link
EP (2) EP1104457B1 (de)
JP (1) JP4448619B2 (de)
AT (1) ATE309342T1 (de)
AU (1) AU769516C (de)
CA (1) CA2339901C (de)
DE (1) DE69928263T2 (de)
DK (1) DK1104457T3 (de)
ES (1) ES2253901T3 (de)
FR (1) FR2782323B1 (de)
IL (2) IL141373A0 (de)
NO (1) NO20015787L (de)
WO (1) WO2000009679A1 (de)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
WO2001029211A2 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Method for directed evolution by random chimeragenesis on transient templates
US6534292B1 (en) * 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
WO2001096551A2 (en) * 2000-06-14 2001-12-20 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
AU2001273559A1 (en) * 2000-07-18 2002-01-30 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
WO2005012515A2 (en) 2003-04-29 2005-02-10 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
AU2002307340A1 (en) 2001-04-16 2002-10-28 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
EP1381680A1 (de) * 2001-04-25 2004-01-21 Proteus Matrizen-vermitteltes, ligation-orientiertes verfahren des nicht zufälligen shuffling von polynukleotiden
FR2824073B1 (fr) * 2001-04-25 2003-12-19 Proteus Procede de creation in vitro de sequences polynucleotidiques recombinees par ligation orientee
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
WO2003010311A2 (en) * 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
EP2315145B1 (de) 2002-03-01 2015-11-25 Codexis Mayflower Holdings, LLC Verfahren, Systeme und Software zur Erkennung von funktionalen Biomolekülen
WO2003078583A2 (en) 2002-03-09 2003-09-25 Maxygen, Inc. Optimization of crossover points for directed evolution
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
EP1497418B1 (de) 2002-04-19 2012-10-17 Verenium Corporation Phospholipasen, diese codierende nukleinsäuren sowie verfahren zu ihrer herstellung und verwendung
ATE356871T1 (de) 2002-05-17 2007-04-15 Alligator Bioscience Ab Eine methode zur in vitro molekularen evolution der proteinfunktion
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
AU2003304275A1 (en) 2002-08-06 2005-01-21 Pioneer Hi-Bred International, Inc. Ap1 amine oxidase variants
EP2194133B1 (de) 2003-03-06 2015-12-02 BASF Enzymes LLC Amylasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
CA2515583C (en) 2003-03-07 2015-07-14 Diversa Corporation Hydrolases, nucleic acids encoding them and methods for making and using them
EP1613733B1 (de) 2003-04-04 2015-06-03 BASF Enzymes LLC Pektatlyasen, diese codierende nukleinsäuren und verfahren zu deren herstellung und verwendung
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
CN103484486B (zh) 2003-07-02 2018-04-24 维莱尼姆公司 葡聚糖酶，编码它们的核酸以及制备和使用它们的方法
US7741089B2 (en) 2003-08-11 2010-06-22 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
WO2005017116A2 (en) 2003-08-11 2005-02-24 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
ES2352172T3 (es) 2004-06-09 2011-02-16 Pioneer-Hi-Bred International, Inc. Péptidos de transito de plastidio.
AU2005264938B2 (en) 2004-06-16 2011-11-24 Verenium Corporation Compositions and methods for enzymatic decolorization of chlorophyll
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
EP1869174B1 (de) 2005-03-10 2015-02-18 BASF Enzymes LLC Lyase-enzyme, nukleinsäuren zu deren kodierung und verfahren zu deren herstellung und verwendung
EA018577B1 (ru) 2005-03-15 2013-09-30 Верениум Корпорейшн Целлюлазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
EP1987142A4 (de) 2006-02-02 2009-07-15 Verenium Corp Esterasen und damit zusammenhängende nukleinsäuren und verfahren
NZ595502A (en) 2006-02-10 2013-11-29 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
ES2682284T3 (es) 2006-02-14 2018-09-19 Bp Corporation North America Inc. Xilanasas, ácidos nucleicos que las codifican y métodos para elaborarlas y usarlas
KR101622894B1 (ko) 2006-03-07 2016-05-19 바스프 엔자임스 엘엘씨 알돌라제, 이것을 코딩하는 핵산과 그 제조 방법 및 사용 방법
ES2416104T3 (es) 2006-03-07 2013-07-30 Cargill, Incorporated Aldolasas, ácidos nucleicos que las codifican y métodos para producir y usar las mismas
EP1842915A1 (de) 2006-04-04 2007-10-10 Libragen In vitro Methode zur Polynukleotide Sequenz Durchmischung durch die Fragmentierung und Ligierung von rekursiv zirkulären DNA Molekülen
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
CN106222185B (zh) 2006-08-04 2021-12-03 维莱尼姆公司 葡聚糖酶、编码它们的核酸及制备和使用它们的方法
EP2069513A4 (de) 2006-08-04 2012-03-21 California Inst Of Techn Verfahren und systeme zur selektiven fluorinierung organischer moleküle
EP2057274B1 (de) 2006-09-21 2013-12-11 DSM IP Assets B.V. Phospholipase ,nukleinsaüre, die diese kodieren und verfahren zur ihrer herstellung und verwendung
US8877478B2 (en) 2006-09-21 2014-11-04 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
PL3101128T3 (pl) 2006-12-21 2019-09-30 Basf Enzymes Llc Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
CN104212822A (zh) 2007-01-30 2014-12-17 Bp法人北美有限公司 用于处理木质纤维素的酶、编码它们的核酸及其制备和应用方法
WO2009001304A1 (en) 2007-06-28 2008-12-31 Firmenich Sa Modified 13-hydroperoxide lyases and uses thereof
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
NZ601191A (en) 2007-10-03 2014-01-31 Verenium Corp Xylanases, nucleic acids encoding them and methods for making and using them
CA2710922A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
AU2008347234B2 (en) 2008-01-03 2015-05-28 Basf Enzymes Llc Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
EP2297310A2 (de) 2008-05-23 2011-03-23 E. I. du Pont de Nemours and Company Neue dgat-gene für gesteigerte samenspeicherlipidproduktion und veränderte fettsäureprofile bei ölsamenpflanzen
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
DK2346995T3 (en) 2008-09-26 2019-02-11 Tocagen Inc GENETERIVE VECTORS AND CYTOSINDEAMINASES
HUE030948T2 (en) 2009-05-21 2017-06-28 Basf Enzymes Llc Phytases, nucleic acids and processes encoding them for their production and use
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
CA2805941A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
WO2013016207A2 (en) 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
US20150184173A1 (en) 2012-05-04 2015-07-02 E I Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
FR2994981B1 (fr) 2012-09-05 2014-09-12 Centre Nat Rech Scient Polypeptide a activite beta-glucosidase renforcee a basse temperature
EP2929027B1 (de) 2012-12-06 2018-02-21 Agilent Technologies, Inc. Molekülherstellung
EP2970995A1 (de) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Zusammensetzungen mit dicamba-decarboxylaseaktivität und verfahren zur verwendung
CA2904537A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
US10006045B2 (en) 2013-08-16 2018-06-26 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR122020001770B1 (pt) 2013-09-13 2022-11-29 Pioneer Hi-Bred International, Inc Construto de dna, método de obtenção de planta transgênica, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto
FR3013731B1 (fr) 2013-11-22 2017-09-01 Ifp Energies Now Variants d'endoglucanases a activite amelioree et leurs utilisations
RU2747978C2 (ru) 2014-02-07 2021-05-18 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CN114763376A (zh) 2014-02-07 2022-07-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
FR3022558B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
FR3022557B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
WO2016061206A1 (en) 2014-10-16 2016-04-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP4663761A3 (de) 2015-01-15 2026-03-04 Pioneer Hi-Bred International, Inc. Insektizidproteine und verfahren zu deren verwendung
MX376048B (es) 2015-03-11 2025-03-07 Corteva Agriscience Llc Combinaciones insecticidas de pip-72 y métodos de uso.
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN116003550A (zh) 2015-08-06 2023-04-25 先锋国际良种公司 植物来源的杀昆虫蛋白及其使用方法
EP3390431A1 (de) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
CN109068660B (zh) 2016-05-04 2023-05-02 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN109788735A (zh) 2016-07-01 2019-05-21 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
CN109863167B (zh) 2016-11-01 2022-12-23 先锋国际良种公司 杀昆虫蛋白及其使用方法
CA3044404A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2019007491A (es) 2016-12-22 2019-09-06 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
RU2019140646A (ru) 2017-05-11 2021-06-11 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CN112020302B9 (zh) 2018-03-14 2023-05-09 先锋国际良种公司 来自植物的杀昆虫蛋白及其使用方法
CA3092075A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CN115151643A (zh) 2020-01-10 2022-10-04 加利福尼亚大学董事会 用于产生橄榄醇酸和橄榄醇酸类似物的生物合成平台
CN115867564A (zh) 2020-07-14 2023-03-28 先锋国际良种公司 杀昆虫蛋白及其使用方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
JP3018163B2 (ja) * 1997-09-04 2000-03-13 工業技術院長 パイロコッカス属に属する超好熱性細菌由来の耐熱性Ｆｌａｐエンドヌクレアーゼ

Also Published As

Publication number Publication date
NO20015787D0 (no) 2001-11-27
EP1683861A1 (de) 2006-07-26
FR2782323A1 (fr) 2000-02-18
JP2002538763A (ja) 2002-11-19
FR2782323B1 (fr) 2002-01-11
WO2000009679A1 (fr) 2000-02-24
EP1104457B1 (de) 2005-11-09
DE69928263D1 (de) 2005-12-15
CA2339901A1 (fr) 2000-02-24
AU5171799A (en) 2000-03-06
IL141373A0 (en) 2002-03-10
AU769516C (en) 2004-11-18
DE69928263T2 (de) 2006-08-10
IL141373A (en) 2008-11-03
DK1104457T3 (da) 2006-03-27
JP4448619B2 (ja) 2010-04-14
EP1104457A1 (de) 2001-06-06
ES2253901T3 (es) 2006-06-01
AU769516B2 (en) 2004-01-29
NO20015787L (no) 2001-11-27
CA2339901C (fr) 2012-02-28

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