ATE309342T1 - Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen - Google Patents

Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen

Info

Publication number
ATE309342T1
ATE309342T1 AT99936725T AT99936725T ATE309342T1 AT E309342 T1 ATE309342 T1 AT E309342T1 AT 99936725 T AT99936725 T AT 99936725T AT 99936725 T AT99936725 T AT 99936725T AT E309342 T1 ATE309342 T1 AT E309342T1
Authority
AT
Austria
Prior art keywords
sequences
library
recombined
sequence
fragments
Prior art date
Application number
AT99936725T
Other languages
English (en)
Inventor
Daniel Dupret
Jean-Michel Masson
Fabrice Lefevre
Original Assignee
Proteus Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus Sa filed Critical Proteus Sa
Application granted granted Critical
Publication of ATE309342T1 publication Critical patent/ATE309342T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT99936725T 1998-08-12 1999-08-11 Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen ATE309342T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9810338A FR2782323B1 (fr) 1998-08-12 1998-08-12 Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
PCT/FR1999/001973 WO2000009679A1 (fr) 1998-08-12 1999-08-11 Procede d'obtention in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues

Publications (1)

Publication Number Publication Date
ATE309342T1 true ATE309342T1 (de) 2005-11-15

Family

ID=9529621

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99936725T ATE309342T1 (de) 1998-08-12 1999-08-11 Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen

Country Status (12)

Country Link
EP (2) EP1104457B1 (de)
JP (1) JP4448619B2 (de)
AT (1) ATE309342T1 (de)
AU (1) AU769516C (de)
CA (1) CA2339901C (de)
DE (1) DE69928263T2 (de)
DK (1) DK1104457T3 (de)
ES (1) ES2253901T3 (de)
FR (1) FR2782323B1 (de)
IL (2) IL141373A0 (de)
NO (1) NO20015787L (de)
WO (1) WO2000009679A1 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
AU1098001A (en) * 1999-10-19 2001-04-30 Enchira Biotechnology Corporation Method for directed evolution by random chimeragenesis on transient templates
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
EP1294869A2 (de) * 2000-06-14 2003-03-26 Diversa Corporation Manipulation von ganzen zellen durch mutation eines grossteils des stargenoms, kombination der mutationen und wahlweises wiederholen
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
WO2002006469A2 (en) * 2000-07-18 2002-01-24 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
AU2002307340A1 (en) 2001-04-16 2002-10-28 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
FR2824073B1 (fr) * 2001-04-25 2003-12-19 Proteus Procede de creation in vitro de sequences polynucleotidiques recombinees par ligation orientee
EP1381680A1 (de) * 2001-04-25 2004-01-21 Proteus Matrizen-vermitteltes, ligation-orientiertes verfahren des nicht zufälligen shuffling von polynukleotiden
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
WO2003010311A2 (en) * 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
HUE028524T2 (en) 2002-03-01 2016-12-28 Codexis Mayflower Holdings Llc Procedures, systems and software for identifying functional biomolecules
EP1488335A4 (de) 2002-03-09 2006-11-15 Maxygen Inc Optimierung von crossover-punkten für die gerichtete evolution
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
EA010903B1 (ru) 2002-04-19 2008-12-30 Дайверса Корпорейшн Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения
CA2485506C (en) 2002-05-17 2012-02-28 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
EP2275536A1 (de) 2002-08-06 2011-01-19 Verdia, Inc. AP1-Aminoxidase-Varianten
CN102618564B (zh) 2003-03-06 2014-10-29 维莱尼姆公司 淀粉酶、编码它们的核酸及其制备和应用方法
EP3299465A1 (de) 2003-03-07 2018-03-28 DSM IP Assets B.V. Hydrolasen, nukleinsäuren zur codierung davon und verfahren zur herstellung und verwendung davon
PL2341136T3 (pl) 2003-04-04 2016-12-30 Liazy pektynianowe, kodujące je kwasy nukleinowe oraz sposoby ich wytwarzania i zastosowania
CA2521284C (en) 2003-04-29 2014-07-08 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
EP1639091B1 (de) 2003-06-17 2012-12-05 California University Of Technology Regio- und enantioselektive Alkanhydroxylierung mit modifiziertem Cytochrom p450
US7960148B2 (en) 2003-07-02 2011-06-14 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
EP1660646B1 (de) 2003-08-11 2014-12-31 California Institute Of Technology Temperaturstabile peroxidgetriebene cytochrom-p450-oxygenase-varianten und verwendungsverfahren
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
EP1753866B1 (de) 2004-06-09 2010-09-22 Pioneer-Hi-Bred International, Inc. Plastiden transit peptide
JP2008505619A (ja) 2004-06-16 2008-02-28 ヴェレニウム コーポレイション 葉緑素を酵素的に脱色するための組成物及び方法
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
US20090038023A1 (en) 2005-03-10 2009-02-05 Verenium Corporation Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them
CN102943085A (zh) 2005-03-15 2013-02-27 维莱尼姆公司 纤维素酶、编码它们的核酸及其制备和应用的方法
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
MY160756A (en) 2006-02-10 2017-03-15 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
EP1989302B1 (de) 2006-02-14 2018-04-11 BP Corporation North America Inc. Xylanasen, dafür kodierende nukleinsäuren sowie verfahren zu deren herstellung und verwendung
EP2112227B1 (de) 2006-03-07 2013-05-15 Cargill, Incorporated Aldolasen, Nukleinsäuren zu ihrer Kodierung sowie Verfahren zu ihrer Herstellung und Verwendung
EP3153580A3 (de) 2006-03-07 2017-04-19 BASF Enzymes LLC Aldolasen, nukleinsäuren zur codierung davon sowie verfahren zur herstellung und verwendung davon
EP1842915A1 (de) 2006-04-04 2007-10-10 Libragen In vitro Methode zur Polynukleotide Sequenz Durchmischung durch die Fragmentierung und Ligierung von rekursiv zirkulären DNA Molekülen
MX369001B (es) 2006-08-04 2019-10-24 Basf Enzymes Llc Glucanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
CN101558154B (zh) 2006-09-21 2016-02-24 帝斯曼知识产权资产管理有限公司 磷脂酶、编码它们的核酸及其制备和应用方法
PT2617823E (pt) 2006-09-21 2015-11-23 Basf Enzymes Llc Fitases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar
PL2069490T5 (pl) 2006-12-21 2018-09-28 Basf Enzymes Llc Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
US20100189706A1 (en) 2007-01-30 2010-07-29 Cathy Chang Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
BRPI0813187B1 (pt) 2007-06-28 2022-05-10 Firmenich Sa 13-hidroperóxido liases modificadas e respectivos usos
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
CN103757036B (zh) 2007-10-03 2019-10-01 维莱尼姆公司 木聚糖酶、编码它们的核酸以及其制备和应用方法
WO2009088753A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
WO2009088949A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
CA2990650C (en) 2008-05-23 2021-02-09 Pioneer Hi-Bred International, Inc. Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
WO2010045002A2 (en) 2008-09-26 2010-04-22 Tocagen Inc. Gene therapy vectors and cytosine deaminases
US9695403B2 (en) 2009-05-21 2017-07-04 Syngenta Participations Ag Phytases, nucleic acids encoding them and methods for making and using them
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
US9139842B2 (en) 2010-08-13 2015-09-22 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
WO2013016207A2 (en) 2011-07-22 2013-01-31 California Institute Of Technology Stable fungal cel6 enzyme variants
CA2872096A1 (en) 2012-05-04 2013-11-07 E. I. Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
FR2994981B1 (fr) 2012-09-05 2014-09-12 Centre Nat Rech Scient Polypeptide a activite beta-glucosidase renforcee a basse temperature
JP6745599B2 (ja) 2012-12-06 2020-08-26 アジレント・テクノロジーズ・インクＡｇｉｌｅｎｔ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃ． 分子の作製
US20160053277A1 (en) 2013-03-14 2016-02-25 Pioneer Hi-Bred International, Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
EP2970935A1 (de) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Zusammensetzungen mit dicamba-decarboxylaseaktivität und verfahren zur verwendung
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
CA2920339C (en) 2013-08-16 2023-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3223359A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
FR3013731B1 (fr) 2013-11-22 2017-09-01 Ifp Energies Now Variants d'endoglucanases a activite amelioree et leurs utilisations
EP3102684B1 (de) 2014-02-07 2020-05-06 Pioneer Hi-Bred International, Inc. Insektizidproteine und verfahren zu deren verwendung
MX2016010187A (es) 2014-02-07 2017-07-11 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
FR3022558B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
FR3022557B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
CN113372421B (zh) 2014-10-16 2024-08-06 先锋国际良种公司 杀昆虫蛋白及其使用方法
UA126544C2 (uk) 2015-01-15 2022-11-02 Піонір Хай-Бред Інтернешнл, Інк. Рекомбінантний полінуклеотид, що кодує інсектицидний поліпептид, та спосіб його застосування
MX376048B (es) 2015-03-11 2025-03-07 Corteva Agriscience Llc Combinaciones insecticidas de pip-72 y métodos de uso.
BR112017024948A2 (pt) 2015-05-19 2018-07-31 Pioneer Hi Bred Int proteínas inseticidas e métodos para uso das mesmas
RU2762832C2 (ru) 2015-08-06 2021-12-23 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки растительного происхождения и способы их применения
WO2017105987A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3960863B1 (de) 2016-05-04 2024-11-13 Pioneer Hi-Bred International, Inc. Insektizide proteine und verfahren zu deren verwendung
WO2018005411A1 (en) 2016-07-01 2018-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
WO2018111551A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11213028B2 (en) 2016-12-22 2022-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
UA126807C2 (uk) 2017-05-11 2023-02-08 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок і спосіб його застосування
WO2019178038A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US11525144B2 (en) 2018-03-14 2022-12-13 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
JP2023509662A (ja) 2020-01-10 2023-03-09 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア オリベトリン酸及びオリベトリン酸類縁体の産生のための生合成プラットフォーム
CN115867564A (zh) 2020-07-14 2023-03-28 先锋国际良种公司 杀昆虫蛋白及其使用方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
JP3018163B2 (ja) * 1997-09-04 2000-03-13 工業技術院長 パイロコッカス属に属する超好熱性細菌由来の耐熱性Ｆｌａｐエンドヌクレアーゼ

Also Published As

Publication number Publication date
EP1104457B1 (de) 2005-11-09
EP1683861A1 (de) 2006-07-26
AU5171799A (en) 2000-03-06
EP1104457A1 (de) 2001-06-06
AU769516C (en) 2004-11-18
WO2000009679A1 (fr) 2000-02-24
IL141373A0 (en) 2002-03-10
CA2339901C (fr) 2012-02-28
NO20015787D0 (no) 2001-11-27
CA2339901A1 (fr) 2000-02-24
NO20015787L (no) 2001-11-27
DE69928263T2 (de) 2006-08-10
DE69928263D1 (de) 2005-12-15
FR2782323A1 (fr) 2000-02-18
IL141373A (en) 2008-11-03
FR2782323B1 (fr) 2002-01-11
DK1104457T3 (da) 2006-03-27
ES2253901T3 (es) 2006-06-01
JP2002538763A (ja) 2002-11-19
AU769516B2 (en) 2004-01-29
JP4448619B2 (ja) 2010-04-14

Similar Documents

Publication Publication Date Title
ATE309342T1 (de) Verfahren zur herstellung in vitro von rekombinierten nucleotidsequenzen, und so hergestelte genbanken und sequenzen
ZA967173B (en) Purified coprinus laccases and nucleic acids and encoding same
DE69829976D1 (de) Verfahren zur trocknung von kompetenten zellen
BR9814404A (pt) " clonagem de expressão em fungos filamentosos "
NO20010019L (no) Karakterisering av genfunksjon ved å benytte dobbeltrådet RNA inhibisjon
DE69731834D1 (de) Agouti verwandtes gen
ATE469223T1 (de) Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
DK1117798T3 (da) Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
DE69728878D1 (de) Verfahren zur vermehrung der hemoproteinherstellung in filamentösen fungi
PT1025245E (pt) Criacao rapida de linhas celulares estaveis de mamifero produzindo elevados iiveis de proteinas recombinantes
ATE395424T1 (de) Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
DE69619808D1 (de) Chimäre msp und daf proteine mit einem telloberflächenlokalisierendem domän
CA2362518A1 (en) Heat-inducible promoter
DE69333304D1 (de) Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen
DE69929708D1 (de) Methode zur herstellung von sätzen komplementärer oligonucleotide
DE502005007057D1 (de) Verfahren zur herstellung von (s)-butan-2-ol
CA2467383A1 (en) Rapidly maturing fluorescent proteins and methods for using the same
DE60117375D1 (de) Verfahren zur erhöhung der expression von einem interessierenden protein durch rekombinante wirtzellen
DE69922071D1 (de) Tumor-suppressorgen Del-27 und das dadurch kodierte Protein, verfahren zu deren Herstellung und Nutzung
DE60032162D1 (de) Regulatorische sequenzen von funktioneller bedeutung in filamentösen pilzen
ATE319821T1 (de) Verfahren und produkt zur regulation der reaktionsempfindlichkeit von zellen auf externe signale
WO2002077023A3 (en) A new essential downstream component of the wingless signalling pathway
WO2002083853A3 (en) Method for enhancing the translation and expression of recombinant proteins
WO2002026978A1 (en) Novel proteins, genes encoding them and method of using the same
WO2002061039A3 (en) Thuja plicata dirigent protein promotors

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1104457

Country of ref document: EP