IL141373A0 - Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences - Google Patents

Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences

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Publication number
IL141373A0
IL141373A0 IL14137399A IL14137399A IL141373A0 IL 141373 A0 IL141373 A0 IL 141373A0 IL 14137399 A IL14137399 A IL 14137399A IL 14137399 A IL14137399 A IL 14137399A IL 141373 A0 IL141373 A0 IL 141373A0
Authority
IL
Israel
Prior art keywords
sequences
library
polynucleotide sequences
sequence
recombined
Prior art date
Application number
IL14137399A
Original Assignee
Proteus Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus Sa filed Critical Proteus Sa
Publication of IL141373A0 publication Critical patent/IL141373A0/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method (I) for producing recombined polynucleotide sequences from a library of double-stranded polynucleotide sequences is new and comprises fragmenting and denaturing library sequences, hybridizing them with assembly matrices and selecting sequences with requires properties.. Method (I) for producing recombined polynucleotide sequences from a library of double-stranded polynucleotide sequences is new and comprises: (1) fragmenting the library sequences; (2) denaturing the fragments, optionally in the presence of one or more assembly matrices; (3) hybridizing the fragments with one or more assembly matrices, if these were not present in step (b); (4) ligating the fragments to obtain recombined sequences; and (5) selecting recombined sequences having advantageous properties compared with one or more reference sequences. Independent claims are also included for the following: (1) a recombined polynucleotide sequence obtained and selected by method (I); (2) a vector containing the sequence of (1); (3) a host cell transformed with the sequence of (1) or the vector of (2); (4) a protein encoded by the sequence of (1); (5) a library of polynucleotide sequences as in (1), vectors as in (2), cells as in (3) or proteins as in (4).
IL14137399A 1998-08-12 1999-08-11 Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences IL141373A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9810338A FR2782323B1 (en) 1998-08-12 1998-08-12 PROCESS FOR THE IN VITRO PRODUCTION OF RECOMBINANT POLYNUCLEOTIDE SEQUENCES, SEQUENCE BANKS AND SEQUENCES THUS OBTAINED
PCT/FR1999/001973 WO2000009679A1 (en) 1998-08-12 1999-08-11 Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences

Publications (1)

Publication Number Publication Date
IL141373A0 true IL141373A0 (en) 2002-03-10

Family

ID=9529621

Family Applications (2)

Application Number Title Priority Date Filing Date
IL14137399A IL141373A0 (en) 1998-08-12 1999-08-11 Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences
IL141373A IL141373A (en) 1998-08-12 2001-02-11 Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL141373A IL141373A (en) 1998-08-12 2001-02-11 Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences

Country Status (12)

Country Link
EP (2) EP1683861A1 (en)
JP (1) JP4448619B2 (en)
AT (1) ATE309342T1 (en)
AU (1) AU769516C (en)
CA (1) CA2339901C (en)
DE (1) DE69928263T2 (en)
DK (1) DK1104457T3 (en)
ES (1) ES2253901T3 (en)
FR (1) FR2782323B1 (en)
IL (2) IL141373A0 (en)
NO (1) NO20015787L (en)
WO (1) WO2000009679A1 (en)

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Also Published As

Publication number Publication date
NO20015787D0 (en) 2001-11-27
DK1104457T3 (en) 2006-03-27
DE69928263D1 (en) 2005-12-15
FR2782323B1 (en) 2002-01-11
NO20015787L (en) 2001-11-27
IL141373A (en) 2008-11-03
AU769516C (en) 2004-11-18
JP4448619B2 (en) 2010-04-14
ES2253901T3 (en) 2006-06-01
EP1104457B1 (en) 2005-11-09
FR2782323A1 (en) 2000-02-18
ATE309342T1 (en) 2005-11-15
EP1104457A1 (en) 2001-06-06
DE69928263T2 (en) 2006-08-10
WO2000009679A1 (en) 2000-02-24
AU5171799A (en) 2000-03-06
AU769516B2 (en) 2004-01-29
CA2339901A1 (en) 2000-02-24
CA2339901C (en) 2012-02-28
EP1683861A1 (en) 2006-07-26
JP2002538763A (en) 2002-11-19

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