AU769516C - Method for obtaining (in vitro) recombined polynucleotide sequences, sequence banks and resulting sequences - Google Patents

Method for obtaining (in vitro) recombined polynucleotide sequences, sequence banks and resulting sequences

Info

Publication number
AU769516C
AU769516C AU51717/99A AU5171799A AU769516C AU 769516 C AU769516 C AU 769516C AU 51717/99 A AU51717/99 A AU 51717/99A AU 5171799 A AU5171799 A AU 5171799A AU 769516 C AU769516 C AU 769516C
Authority
AU
Australia
Prior art keywords
sequences
library
recombined
polynucleotide sequences
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU51717/99A
Other versions
AU5171799A (en
AU769516B2 (en
Inventor
Daniel Dupret
Fabrice Lefevre
Jean-Michel Masson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Proteus SA
Original Assignee
Proteus SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus SA filed Critical Proteus SA
Publication of AU5171799A publication Critical patent/AU5171799A/en
Publication of AU769516B2 publication Critical patent/AU769516B2/en
Application granted granted Critical
Publication of AU769516C publication Critical patent/AU769516C/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)

Abstract

A method (I) for producing recombined polynucleotide sequences from a library of double-stranded polynucleotide sequences is new and comprises fragmenting and denaturing library sequences, hybridizing them with assembly matrices and selecting sequences with requires properties.. Method (I) for producing recombined polynucleotide sequences from a library of double-stranded polynucleotide sequences is new and comprises: (1) fragmenting the library sequences; (2) denaturing the fragments, optionally in the presence of one or more assembly matrices; (3) hybridizing the fragments with one or more assembly matrices, if these were not present in step (b); (4) ligating the fragments to obtain recombined sequences; and (5) selecting recombined sequences having advantageous properties compared with one or more reference sequences. Independent claims are also included for the following: (1) a recombined polynucleotide sequence obtained and selected by method (I); (2) a vector containing the sequence of (1); (3) a host cell transformed with the sequence of (1) or the vector of (2); (4) a protein encoded by the sequence of (1); (5) a library of polynucleotide sequences as in (1), vectors as in (2), cells as in (3) or proteins as in (4).
AU51717/99A 1998-08-12 1999-08-11 Method for obtaining (in vitro) recombined polynucleotide sequences, sequence banks and resulting sequences Ceased AU769516C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9810338 1998-08-12
FR9810338A FR2782323B1 (en) 1998-08-12 1998-08-12 PROCESS FOR THE IN VITRO PRODUCTION OF RECOMBINANT POLYNUCLEOTIDE SEQUENCES, SEQUENCE BANKS AND SEQUENCES THUS OBTAINED
PCT/FR1999/001973 WO2000009679A1 (en) 1998-08-12 1999-08-11 Method for obtaining in vitro recombined polynucleotide sequences, sequence banks and resulting sequences

Publications (3)

Publication Number Publication Date
AU5171799A AU5171799A (en) 2000-03-06
AU769516B2 AU769516B2 (en) 2004-01-29
AU769516C true AU769516C (en) 2004-11-18

Family

ID=9529621

Family Applications (1)

Application Number Title Priority Date Filing Date
AU51717/99A Ceased AU769516C (en) 1998-08-12 1999-08-11 Method for obtaining (in vitro) recombined polynucleotide sequences, sequence banks and resulting sequences

Country Status (12)

Country Link
EP (2) EP1683861A1 (en)
JP (1) JP4448619B2 (en)
AT (1) ATE309342T1 (en)
AU (1) AU769516C (en)
CA (1) CA2339901C (en)
DE (1) DE69928263T2 (en)
DK (1) DK1104457T3 (en)
ES (1) ES2253901T3 (en)
FR (1) FR2782323B1 (en)
IL (2) IL141373A0 (en)
NO (1) NO20015787L (en)
WO (1) WO2000009679A1 (en)

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Also Published As

Publication number Publication date
EP1104457B1 (en) 2005-11-09
NO20015787D0 (en) 2001-11-27
NO20015787L (en) 2001-11-27
JP4448619B2 (en) 2010-04-14
EP1104457A1 (en) 2001-06-06
CA2339901A1 (en) 2000-02-24
JP2002538763A (en) 2002-11-19
CA2339901C (en) 2012-02-28
IL141373A0 (en) 2002-03-10
DK1104457T3 (en) 2006-03-27
AU5171799A (en) 2000-03-06
EP1683861A1 (en) 2006-07-26
DE69928263D1 (en) 2005-12-15
DE69928263T2 (en) 2006-08-10
WO2000009679A1 (en) 2000-02-24
AU769516B2 (en) 2004-01-29
FR2782323B1 (en) 2002-01-11
ES2253901T3 (en) 2006-06-01
ATE309342T1 (en) 2005-11-15
FR2782323A1 (en) 2000-02-18
IL141373A (en) 2008-11-03

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