AU1098001A - Method for directed evolution by random chimeragenesis on transient templates - Google Patents

Method for directed evolution by random chimeragenesis on transient templates

Info

Publication number
AU1098001A
AU1098001A AU10980/01A AU1098001A AU1098001A AU 1098001 A AU1098001 A AU 1098001A AU 10980/01 A AU10980/01 A AU 10980/01A AU 1098001 A AU1098001 A AU 1098001A AU 1098001 A AU1098001 A AU 1098001A
Authority
AU
Australia
Prior art keywords
directed evolution
transient templates
random chimeragenesis
chimeragenesis
templates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU10980/01A
Inventor
Joseph J. Arensdorf
Wayne M. Coco
Michael J. Crist
Aldis Darzins
Lance P. Encell
Brian R. Folsom
Hans J. Hektor
William E. Levinson
Mark S. Nalty
Stephen R. Palmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enchira Biotechnology Corp
Original Assignee
Enchira Biotechnology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enchira Biotechnology Corp filed Critical Enchira Biotechnology Corp
Publication of AU1098001A publication Critical patent/AU1098001A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU10980/01A 1999-10-19 2000-10-19 Method for directed evolution by random chimeragenesis on transient templates Abandoned AU1098001A (en)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US16042099P 1999-10-19 1999-10-19
US60160420 1999-10-19
US51466000A 2000-02-29 2000-02-29
US09514660 2000-02-29
US61869600A 2000-07-18 2000-07-18
US21908700P 2000-07-18 2000-07-18
US21888300P 2000-07-18 2000-07-18
US21909000P 2000-07-18 2000-07-18
US61893500A 2000-07-18 2000-07-18
US09618696 2000-07-18
US60219087 2000-07-18
US60219090 2000-07-18
US60218883 2000-07-18
US09618935 2000-07-18
PCT/US2000/029046 WO2001029211A2 (en) 1999-10-19 2000-10-19 Method for directed evolution by random chimeragenesis on transient templates

Publications (1)

Publication Number Publication Date
AU1098001A true AU1098001A (en) 2001-04-30

Family

ID=27569069

Family Applications (1)

Application Number Title Priority Date Filing Date
AU10980/01A Abandoned AU1098001A (en) 1999-10-19 2000-10-19 Method for directed evolution by random chimeragenesis on transient templates

Country Status (2)

Country Link
AU (1) AU1098001A (en)
WO (1) WO2001029211A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU2001273559A1 (en) * 2000-07-18 2002-01-30 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
AU2002254872A1 (en) * 2001-05-10 2002-11-18 Novozymes A/S A method for producing recombined polynucleotides
US8067556B2 (en) 2001-07-26 2011-11-29 Agilent Technologies, Inc. Multi-site mutagenesis
EP1281757A1 (en) * 2001-07-31 2003-02-05 Direvo Biotech AG Method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids
US20040005709A1 (en) * 2001-10-24 2004-01-08 Hoogenboom Henricus Renerus Jacobus Mattheus Hybridization control of sequence variation
DK1493027T3 (en) 2002-03-01 2014-11-17 Codexis Mayflower Holdings Llc Methods, systems and software for identifying functional biomolecules
AU2003213846A1 (en) 2002-03-09 2003-09-29 Maxygen, Inc. Optimization of crossover points for directed evolution
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
CN104297221B (en) * 2014-09-28 2017-01-25 南京诺唯赞生物科技有限公司 Active determination method for Lambda exonuclease
CA2997929A1 (en) 2015-09-08 2017-03-16 Cold Spring Harbor Laboratory Genetic copy number determination using high throughput multiplex sequencing of smashed nucleotides
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
WO2023132885A1 (en) * 2022-01-04 2023-07-13 Modernatx, Inc. Methods of purifying dna for gene synthesis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
DE3936258C1 (en) * 1989-10-31 1991-04-25 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften Ev, 3400 Goettingen, De
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
FR2782323B1 (en) * 1998-08-12 2002-01-11 Proteus PROCESS FOR THE IN VITRO PRODUCTION OF RECOMBINANT POLYNUCLEOTIDE SEQUENCES, SEQUENCE BANKS AND SEQUENCES THUS OBTAINED

Also Published As

Publication number Publication date
WO2001029211A2 (en) 2001-04-26
WO2001029211A3 (en) 2002-01-24
WO2001029211A9 (en) 2002-08-15

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase