DK1104457T3 - Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser - Google Patents

Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser

Info

Publication number
DK1104457T3
DK1104457T3 DK99936725T DK99936725T DK1104457T3 DK 1104457 T3 DK1104457 T3 DK 1104457T3 DK 99936725 T DK99936725 T DK 99936725T DK 99936725 T DK99936725 T DK 99936725T DK 1104457 T3 DK1104457 T3 DK 1104457T3
Authority
DK
Denmark
Prior art keywords
sequences
library
polynucleotide sequences
recombined
sequence
Prior art date
Application number
DK99936725T
Other languages
English (en)
Inventor
Daniel Dupret
Jean-Michel Masson
Fabrice Lefevre
Original Assignee
Proteus Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus Sa filed Critical Proteus Sa
Application granted granted Critical
Publication of DK1104457T3 publication Critical patent/DK1104457T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245Escherichia (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK99936725T 1998-08-12 1999-08-11 Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser DK1104457T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9810338A FR2782323B1 (fr) 1998-08-12 1998-08-12 Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues

Publications (1)

Publication Number Publication Date
DK1104457T3 true DK1104457T3 (da) 2006-03-27

Family

ID=9529621

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99936725T DK1104457T3 (da) 1998-08-12 1999-08-11 Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser

Country Status (12)

Country Link
EP (2) EP1683861A1 (da)
JP (1) JP4448619B2 (da)
AT (1) ATE309342T1 (da)
AU (1) AU769516C (da)
CA (1) CA2339901C (da)
DE (1) DE69928263T2 (da)
DK (1) DK1104457T3 (da)
ES (1) ES2253901T3 (da)
FR (1) FR2782323B1 (da)
IL (2) IL141373A0 (da)
NO (1) NO20015787D0 (da)
WO (1) WO2000009679A1 (da)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
WO2001029211A2 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Method for directed evolution by random chimeragenesis on transient templates
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
CA2413022A1 (en) * 2000-06-14 2001-12-20 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
WO2002006469A2 (en) * 2000-07-18 2002-01-24 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
AU2004260931B9 (en) 2003-04-29 2012-01-19 E.I. Du Pont De Nemours And Company Novel glyphosate-N-acetyltransferase (GAT) genes
AU2002307340A1 (en) 2001-04-16 2002-10-28 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
AU2002338443B2 (en) * 2001-04-25 2007-07-19 Proteus Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
FR2824073B1 (fr) * 2001-04-25 2003-12-19 Proteus Procede de creation in vitro de sequences polynucleotidiques recombinees par ligation orientee
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
US20050130140A1 (en) * 2001-07-23 2005-06-16 Bovenberg Roelof A.L. Process for preparing variant polynucleotides
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
ES2564570T3 (es) 2002-03-01 2016-03-23 Codexis Mayflower Holdings, Llc Métodos, sistemas y software para la identificación de biomoléculas funcionales
JP4851687B2 (ja) 2002-03-09 2012-01-11 マキシジェン, インコーポレイテッド 定向進化のための交叉点の最適化
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
EP2298871A1 (en) 2002-04-19 2011-03-23 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
CA2494798A1 (en) 2002-08-06 2005-01-13 Verdia, Inc. Ap1 amine oxidase variants
EP3023498B1 (en) 2003-03-06 2018-11-28 BASF Enzymes LLC Amylases, nucleic acids encoding them and methods for making and using them
EP3299465A1 (en) 2003-03-07 2018-03-28 DSM IP Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
EP1613733B1 (en) 2003-04-04 2015-06-03 BASF Enzymes LLC Pectate lyases, nucleic acids encoding them and methods for making and using them
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
JP2007529993A (ja) 2003-07-02 2007-11-01 シンジェンタ パーティシペーションズ アーゲー グルカナーゼ、それらをコードする核酸並びにそれらを製造及び使用する方法
WO2005017116A2 (en) 2003-08-11 2005-02-24 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
CA2734624A1 (en) 2004-06-09 2005-12-29 Pioneer Hi-Bred International, Inc. Plastid transit peptides
EP2468853B1 (en) 2004-06-16 2015-04-01 DSM IP Assets B.V. Method for enzymatic decolorization of pheophytin
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
EP1869174B1 (en) 2005-03-10 2015-02-18 BASF Enzymes LLC Lyase enzymes, nucleic acids encoding them and methods for making and using them
EP1861506B1 (en) 2005-03-15 2015-04-15 BP Corporation North America Inc. Cellulases, nucleic acids encoding them and methods for making and using them
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
WO2007092314A2 (en) 2006-02-02 2007-08-16 Verenium Corporation Esterases and related nucleic acids and methods
NZ595501A (en) 2006-02-10 2013-05-31 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
US8043839B2 (en) 2006-02-14 2011-10-25 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
CN101443446B (zh) 2006-03-07 2013-08-21 维莱尼姆公司 醛缩酶、编码它们的核酸及制备和使用它们的方法
CN101437954B (zh) 2006-03-07 2015-09-09 嘉吉公司 醛缩酶和编码醛缩酶的核酸以及它们的制备和使用方法
EP1842915A1 (en) 2006-04-04 2007-10-10 Libragen Method of in vitro polynucleotide sequences shuffling by recursive circular DNA molecules fragmentation and ligation
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
MX369001B (es) 2006-08-04 2019-10-24 Basf Enzymes Llc Glucanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
PT2397486E (pt) 2006-09-21 2015-03-23 Basf Enzymes Llc Fitases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar
MX2009003034A (es) 2006-09-21 2009-11-18 Verenium Corp Fosfolipasas, acidos nucleicos que las codifican, y metodos de hacerlas y usarlas.
ES2620288T3 (es) 2006-12-21 2017-06-28 Basf Enzymes Llc Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas
BRPI0807132A2 (pt) 2007-01-30 2018-12-04 Syngenta Participations Ag enzimas para o tratamento de lignocelulósicos, ácidos nucléicos que codificam as mesmas, e metodos e uso das mesmas
BRPI0813187B1 (pt) 2007-06-28 2022-05-10 Firmenich Sa 13-hidroperóxido liases modificadas e respectivos usos
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
EP2205744B1 (en) 2007-10-03 2015-01-14 BP Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
US8541220B2 (en) 2008-01-03 2013-09-24 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
JP5563990B2 (ja) 2008-01-03 2014-07-30 ヴェレニウム コーポレイション トランスフェラーゼおよびオキシドレダクターゼ、それらをコードする核酸並びにそれらを製造および使用する方法
EP3115459A3 (en) 2008-05-23 2017-04-26 E. I. du Pont de Nemours and Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
NZ592070A (en) 2008-09-26 2012-07-27 Tocagen Inc Gene therapy vectors and humanized yeast cytosine deaminase triple mutant
CN106011159A (zh) 2009-05-21 2016-10-12 巴斯夫酶有限责任公司 肌醇六磷酸酶、编码它们的核酸及制备和使用它们的方法
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
US9187762B2 (en) 2010-08-13 2015-11-17 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
US20150184173A1 (en) 2012-05-04 2015-07-02 E I Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
FR2994981B1 (fr) 2012-09-05 2014-09-12 Centre Nat Rech Scient Polypeptide a activite beta-glucosidase renforcee a basse temperature
WO2014088693A1 (en) 2012-12-06 2014-06-12 Agilent Technologies, Inc. Molecular fabrication
US20160040149A1 (en) 2013-03-14 2016-02-11 Pioneer Hi-Bred International Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
US20140289906A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
EP2971000A4 (en) 2013-03-15 2016-11-23 Pioneer Hi Bred Int PHI-4 POLYPEPTIDES AND METHOD FOR THEIR USE
US10006045B2 (en) 2013-08-16 2018-06-26 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3175967A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
FR3013731B1 (fr) 2013-11-22 2017-09-01 Ifp Energies Now Variants d'endoglucanases a activite amelioree et leurs utilisations
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
CA2939156A1 (en) 2014-02-07 2015-08-13 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
FR3022557B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
FR3022558B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
CN113372421A (zh) 2014-10-16 2021-09-10 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN107529763B (zh) 2015-03-11 2021-08-20 先锋国际良种公司 Pip-72的杀昆虫组合及使用方法
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
US11198709B2 (en) 2015-08-06 2021-12-14 E. I. Du Pont De Nemours And Company Plant derived insecticidal proteins and methods for their use
EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2018013249A (es) 2016-05-04 2019-02-13 Pioneer Hi Bred Int Proteinas insecticidas y metodos para sus usos.
US11155829B2 (en) 2016-07-01 2021-10-26 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US11021716B2 (en) 2016-11-01 2021-06-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3044404A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20190390219A1 (en) 2017-02-08 2019-12-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
CN110621780B (zh) 2017-05-11 2024-03-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
US20230235352A1 (en) 2020-07-14 2023-07-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
JP3018163B2 (ja) * 1997-09-04 2000-03-13 工業技術院長 パイロコッカス属に属する超好熱性細菌由来の耐熱性Flapエンドヌクレアーゼ

Also Published As

Publication number Publication date
ES2253901T3 (es) 2006-06-01
IL141373A0 (en) 2002-03-10
FR2782323A1 (fr) 2000-02-18
NO20015787L (no) 2001-11-27
EP1104457A1 (fr) 2001-06-06
AU769516B2 (en) 2004-01-29
AU5171799A (en) 2000-03-06
DE69928263T2 (de) 2006-08-10
EP1683861A1 (fr) 2006-07-26
IL141373A (en) 2008-11-03
CA2339901A1 (fr) 2000-02-24
ATE309342T1 (de) 2005-11-15
WO2000009679A1 (fr) 2000-02-24
JP4448619B2 (ja) 2010-04-14
DE69928263D1 (de) 2005-12-15
FR2782323B1 (fr) 2002-01-11
CA2339901C (fr) 2012-02-28
AU769516C (en) 2004-11-18
EP1104457B1 (fr) 2005-11-09
NO20015787D0 (no) 2001-11-27
JP2002538763A (ja) 2002-11-19

Similar Documents

Publication Publication Date Title
DK1104457T3 (da) Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser
ZA967173B (en) Purified coprinus laccases and nucleic acids and encoding same
CA2293819A1 (en) A method for in vitro molecular evolution of protein function
ATE342359T1 (de) Design von beta-faltblatt-proteinen mit spezifischen bindungseigenschaften
DK0762897T3 (da) Fremgangsmåde til forebyggelse af aggregering af proteiner/peptider ved rehydratisering eller optøning
DK1484338T3 (da) Fremgangsmåder og sammensætninger til inhibition af neoplastisk cellevækst
AU2002360331A1 (en) Egviii endoglucanase and nucleic acids encoding the same
PT1025245E (pt) Criacao rapida de linhas celulares estaveis de mamifero produzindo elevados iiveis de proteinas recombinantes
SI1675956T1 (sl) Minimalno zaporedje dna, ki deluje kot izolirni element kromatina in njegova uporaba pri ekspresiji proteinov
DE69535696D1 (de) Tripeptidyl-aminopeptidase
DK1117798T3 (da) Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
DK0724637T4 (da) Kortikotropinfrigørende faktor2-receptorer
DE69619808D1 (de) Chimäre msp und daf proteine mit einem telloberflächenlokalisierendem domän
DE69333304D1 (de) Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
CA2390685A1 (en) Methods and compositions for inhibiting neoplastic cell growth
WO2002046408A3 (en) Human proteins, polynucleotides encoding them and methods of using the same
RU2004118303A (ru) Быстросозревающие флуоресцирующие белки и способы их применения
WO2002059309A3 (en) Anthozoa derived chromoproteins, fluorescent mutants thereof and methods for using the same
WO2004113539A3 (fr) Procede de production d’une proteine recombinante d’interet et proteine produite
WO2001083552A3 (en) Human sez6 nucleic acids and polypeptides
WO2002083853A3 (en) Method for enhancing the translation and expression of recombinant proteins
WO2002026978A1 (fr) Nouvelles proteines, genes codant ces proteines et leur procede d'utilisation
WO2002044211A3 (en) Endozepine like protein, polynucleotide encoding them and methods of using the same
EP2075335A3 (en) Methods and compositions for inhibiting neoplastic cell growth