WO2002059309A3 - Anthozoa derived chromoproteins, fluorescent mutants thereof and methods for using the same - Google Patents

Anthozoa derived chromoproteins, fluorescent mutants thereof and methods for using the same Download PDF

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Publication number
WO2002059309A3
WO2002059309A3 PCT/US2001/047995 US0147995W WO02059309A3 WO 2002059309 A3 WO2002059309 A3 WO 2002059309A3 US 0147995 W US0147995 W US 0147995W WO 02059309 A3 WO02059309 A3 WO 02059309A3
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WO
WIPO (PCT)
Prior art keywords
proteins
mutants
nucleic acid
fluorescent
subject
Prior art date
Application number
PCT/US2001/047995
Other languages
French (fr)
Other versions
WO2002059309A2 (en
Inventor
Sergey Anatolievich Lukyanov
Konstantin Anatolievi Lukyanov
Arcady Fedorovich Fradkov
Original Assignee
Clontech Lab Inc
Sergey Anatolievich Lukyanov
Konstantin Anatolievi Lukyanov
Arcady Fedorovich Fradkov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clontech Lab Inc, Sergey Anatolievich Lukyanov, Konstantin Anatolievi Lukyanov, Arcady Fedorovich Fradkov filed Critical Clontech Lab Inc
Publication of WO2002059309A2 publication Critical patent/WO2002059309A2/en
Publication of WO2002059309A3 publication Critical patent/WO2002059309A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Nucleic acid compositions encoding Actiniidaen, e.g., Condylactin, chromoproteins and fluorescent mutants thereof, as well as the polypeptide compositions encoded by the same, are provided. The proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins, including non-aggregating mutants and mutants with modulated oligomerization characteristics as compared to wild type. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
PCT/US2001/047995 2000-12-13 2001-12-11 Anthozoa derived chromoproteins, fluorescent mutants thereof and methods for using the same WO2002059309A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25553300P 2000-12-13 2000-12-13
US60/255,533 2000-12-13

Publications (2)

Publication Number Publication Date
WO2002059309A2 WO2002059309A2 (en) 2002-08-01
WO2002059309A3 true WO2002059309A3 (en) 2003-03-13

Family

ID=22968755

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/047995 WO2002059309A2 (en) 2000-12-13 2001-12-11 Anthozoa derived chromoproteins, fluorescent mutants thereof and methods for using the same

Country Status (1)

Country Link
WO (1) WO2002059309A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7005511B2 (en) * 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
RU2004118848A (en) * 2001-12-26 2005-04-20 Клонтек Лэборетериз, Инк. (Us) MUTANT CHROMOPHORES / FLUOROPHORES AND METHODS FOR THEIR PRODUCTION AND APPLICATION
JP4452619B2 (en) 2002-08-23 2010-04-21 独立行政法人理化学研究所 Chromoprotein and fluorescent protein
DK1576157T3 (en) 2002-12-26 2010-11-01 Zakrytoe Aktsionernoe Obschest Fluorescent proteins from Copepode species and methods for using the same

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000034318A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034320A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminiscent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034324A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034321A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034326A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2002030965A2 (en) * 2000-10-12 2002-04-18 Clontech Laboratories, Inc. Nucleic acids encoding stichodactylidae chromoproteins

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000034318A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034320A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminiscent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034324A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034319A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034321A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2000034326A1 (en) * 1998-12-11 2000-06-15 Clontech Laboratories, Inc. Fluorescent proteins from non-bioluminescent species of class anthozoa, genes encoding such proteins and uses thereof
WO2002030965A2 (en) * 2000-10-12 2002-04-18 Clontech Laboratories, Inc. Nucleic acids encoding stichodactylidae chromoproteins

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
FRADKOV A F ET AL.: "Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence", FEBS LETTERS, vol. 479, no. 3, 18 August 2000 (2000-08-18), XP002208900, ISSN: 0014-5793 *
GURSKAYA N G ET AL: "GFP-like chromoproteins as a source of far-red fluorescent proteins", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 507, no. 1, 19 October 2001 (2001-10-19), pages 16 - 20, XP004311103, ISSN: 0014-5793 *
LUKYANOV K A ET AL.: "Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 34, 25 August 2000 (2000-08-25), pages 25879 - 25882, XP002220954, ISSN: 0021-9258, DOI: doi:10.1074/jbc.C000338200 *
MATZ M V ET AL.: "Fluorescent proteins from nonbioluminescent Anthozoa species", NATURE BIOTECHNOLOGIE, vol. 17, no. 10, October 1999 (1999-10-01), XP000882891, ISSN: 1087-0156 *
YANUSHEVICH Y G ET AL: "A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 511, no. 1-3, 30 January 2002 (2002-01-30), pages 11 - 14, XP004334763, ISSN: 0014-5793 *

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