ATE254663T1 - Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen - Google Patents

Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen

Info

Publication number
ATE254663T1
ATE254663T1 AT93921939T AT93921939T ATE254663T1 AT E254663 T1 ATE254663 T1 AT E254663T1 AT 93921939 T AT93921939 T AT 93921939T AT 93921939 T AT93921939 T AT 93921939T AT E254663 T1 ATE254663 T1 AT E254663T1
Authority
AT
Austria
Prior art keywords
eukaryotic cells
secreted proteins
dna sequence
recombinant eukaryotic
increased production
Prior art date
Application number
AT93921939T
Other languages
English (en)
Inventor
Sirkka Keraenen
Markku Aalto
Mika Outola
Hans Ronne
Merja Penttilae
Original Assignee
Valtion Teknillinen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen filed Critical Valtion Teknillinen
Application granted granted Critical
Publication of ATE254663T1 publication Critical patent/ATE254663T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Seasonings (AREA)
AT93921939T 1992-10-06 1993-10-06 Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen ATE254663T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI924494A FI924494A0 (fi) 1992-10-06 1992-10-06 Oekad produktion av avsoendrarde proteiner i eukaryotiska rekombinantceller
PCT/FI1993/000402 WO1994008024A1 (en) 1992-10-06 1993-10-06 Increased production of secreted proteins by recombinant eukaryotic cells

Publications (1)

Publication Number Publication Date
ATE254663T1 true ATE254663T1 (de) 2003-12-15

Family

ID=8535988

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93921939T ATE254663T1 (de) 1992-10-06 1993-10-06 Erhöhte produktion von sekretierten proteinen durch rekombinante eukaryotische zellen

Country Status (14)

Country Link
US (1) US5789193A (de)
EP (1) EP0665890B1 (de)
JP (1) JP3638599B2 (de)
AT (1) ATE254663T1 (de)
AU (1) AU678402B2 (de)
CA (1) CA2146240C (de)
DE (1) DE69333304T2 (de)
DK (1) DK0665890T3 (de)
EE (1) EE03074B1 (de)
ES (1) ES2211870T3 (de)
FI (2) FI924494A0 (de)
NO (1) NO319132B1 (de)
NZ (1) NZ256425A (de)
WO (1) WO1994008024A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922561A (en) * 1994-10-03 1999-07-13 Novo Nordisk Biotech, Inc. Genes encoding signal recognition particle of Aspergillus niger
DE19920712A1 (de) * 1999-05-05 2000-11-09 Rhein Biotech Proz & Prod Gmbh Verfahren zum Herstellen eines rekombinanten Proteines
WO2005083060A1 (en) 2004-03-01 2005-09-09 Kitakyushu Foundation For The Advancement Of Industry, Science And Technology Human cell strains for protein production, provided by selecting strains with high intracellular protein and mutating with carcinogens
US20090247609A1 (en) * 2007-12-20 2009-10-01 Hitto Kaufmann Sm-protein based secretion engineering
EP3020273B1 (de) * 2014-11-17 2018-07-04 Competence Centre on Health Technologies Verfahren zur herstellung biotechnologischer arzneimittel unter verwendung transgener rinder
US11913046B2 (en) 2018-04-23 2024-02-27 Inbiose N.V. Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
EP4194560A1 (de) * 2021-12-08 2023-06-14 European Molecular Biology Laboratory Verbesserte produktion von sekretierten proteinen in pilzzellen
CN114717124B (zh) * 2022-04-19 2024-07-12 天津科技大学 一株高产麦角甾醇的酿酒酵母工程菌株、构建方法及应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3888561T2 (de) * 1987-03-23 1994-09-01 Zymogenetics Inc Hohe Proteinsyntheserate in Hefe.

Also Published As

Publication number Publication date
NO951315L (no) 1995-06-06
DE69333304D1 (de) 2003-12-24
ES2211870T3 (es) 2004-07-16
NO951315D0 (no) 1995-04-05
EP0665890A1 (de) 1995-08-09
EE03074B1 (et) 1998-02-16
EP0665890B1 (de) 2003-11-19
NO319132B1 (no) 2005-06-20
AU5112793A (en) 1994-04-26
FI924494A0 (fi) 1992-10-06
CA2146240A1 (en) 1994-04-14
US5789193A (en) 1998-08-04
JP3638599B2 (ja) 2005-04-13
CA2146240C (en) 2007-05-01
AU678402B2 (en) 1997-05-29
DK0665890T3 (da) 2004-03-08
DE69333304T2 (de) 2004-08-26
FI951633A0 (fi) 1995-04-05
NZ256425A (en) 1996-08-27
FI951633L (fi) 1995-04-05
JPH08501695A (ja) 1996-02-27
WO1994008024A1 (en) 1994-04-14
FI114482B (fi) 2004-10-29

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