EP0482125A1 - Mikroeinspritzung mit aerosolstrahl - Google Patents

Mikroeinspritzung mit aerosolstrahl

Info

Publication number
EP0482125A1
EP0482125A1 EP19900917903 EP90917903A EP0482125A1 EP 0482125 A1 EP0482125 A1 EP 0482125A1 EP 19900917903 EP19900917903 EP 19900917903 EP 90917903 A EP90917903 A EP 90917903A EP 0482125 A1 EP0482125 A1 EP 0482125A1
Authority
EP
European Patent Office
Prior art keywords
particles
aerosol
cell
housing
conduit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19900917903
Other languages
English (en)
French (fr)
Inventor
Laurens J. Mets
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotechnology Research and Development Corp
Original Assignee
Biotechnology Research and Development Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotechnology Research and Development Corp filed Critical Biotechnology Research and Development Corp
Publication of EP0482125A1 publication Critical patent/EP0482125A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
EP19900917903 1989-07-11 1990-06-27 Mikroeinspritzung mit aerosolstrahl Withdrawn EP0482125A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37825689A 1989-07-11 1989-07-11
US378256 1989-07-11

Publications (1)

Publication Number Publication Date
EP0482125A1 true EP0482125A1 (de) 1992-04-29

Family

ID=23492375

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19900917903 Withdrawn EP0482125A1 (de) 1989-07-11 1990-06-27 Mikroeinspritzung mit aerosolstrahl

Country Status (4)

Country Link
EP (1) EP0482125A1 (de)
AU (1) AU642889B2 (de)
IE (1) IE61705B1 (de)
WO (1) WO1991000915A1 (de)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141131A (en) * 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
ATE130371T1 (de) * 1989-12-19 1995-12-15 Ciba Geigy Ag Verfahren und vorrichtung zur genetischen transformation von zellen.
WO1992004439A1 (en) * 1990-08-30 1992-03-19 Brian John Bellhouse Ballistic apparatus
AU1663392A (en) * 1991-03-06 1992-10-06 Agracetus, Inc. Particle mediated transformation of cotton
WO1993007256A1 (en) * 1991-10-07 1993-04-15 Ciba-Geigy Ag Particle gun for introducing dna into intact cells
WO1993023552A1 (en) * 1992-05-21 1993-11-25 Government Of The United States As Represented By Secretary Department Of Health And Human Services Targeting gene expression to living tissue using jet injection
DE4418965A1 (de) * 1994-05-31 1995-12-07 Boehringer Ingelheim Int Verfahren zum Einbringen von Nukleinsäure in höhere eukaryotische Zellen
GB9416663D0 (en) * 1994-08-17 1994-10-12 Oxford Bioscience Limited Particle delivery
US6433154B1 (en) * 1997-06-12 2002-08-13 Bristol-Myers Squibb Company Functional receptor/kinase chimera in yeast cells
US6562623B1 (en) 1999-07-21 2003-05-13 Immunoporation Ltd Method for introducing a substance into a cell
EP1250447B1 (de) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Verfahren, Medien und Vorrichtung zur Einführung von Molekülen in Pflanzenzellen und Bakterien mittels Aerosolstrahlen
US7314969B2 (en) 1999-11-29 2008-01-01 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
AU2002239343B2 (en) * 1999-11-29 2007-10-04 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
CA2409093C (en) 2000-05-16 2009-07-21 Regents Of The University Of Minnesota High mass throughput particle generation using multiple nozzle spraying
DE60333435D1 (de) 2003-02-03 2010-09-02 Bioware Technology Co Ltd Genpistole mit Niederdruckgasbeschleunigung
WO2004074462A2 (en) 2003-02-20 2004-09-02 Athenix Corporation Delta-endotoxin genes and methods for their use
US7674959B2 (en) 2005-04-01 2010-03-09 Athenix Corporation Axmi-027, axmi-036 and axmi-038, a family of delta-endotoxin genes and methods for their use
DE102005061371A1 (de) 2005-12-14 2007-06-28 Eberhard-Karls-Universität Tübingen Generierung von biologischem Material unter Verwendung eines Nährstoffverneblungsverfahrens in Kombination mit zellbeeinflussenden biologischen und physikalischen Stimuli
US20070173470A1 (en) * 2006-01-23 2007-07-26 Chi-Hung Lin Methods for delivering extracellular target into cells
US7951428B2 (en) 2006-01-31 2011-05-31 Regents Of The University Of Minnesota Electrospray coating of objects
US9108217B2 (en) 2006-01-31 2015-08-18 Nanocopoeia, Inc. Nanoparticle coating of surfaces
WO2007089883A2 (en) 2006-01-31 2007-08-09 Nanocopoeia, Inc. Nanoparticle coating of surfaces
EP2455393A3 (de) 2006-06-15 2012-08-22 Athenix Corporation Pestizidproteinfamilie und Verwendungsverfahren dafür
US9040816B2 (en) 2006-12-08 2015-05-26 Nanocopoeia, Inc. Methods and apparatus for forming photovoltaic cells using electrospray
CN101889090B (zh) * 2007-10-05 2018-01-23 陶氏益农公司 用于将分子物质转移入植物细胞中的方法
WO2009049126A2 (en) 2007-10-10 2009-04-16 Athenix Corporation Synthetic genes encoding cry1ac
WO2009052242A2 (en) 2007-10-16 2009-04-23 Athenix Corporation Axmi-066 and axmi-076: delta-endotoxin proteins and methods for their use
AU2009262153B2 (en) 2008-06-25 2014-02-27 BASF Agricultural Solutions Seed US LLC Toxin genes and methods for their use
EP2297190A2 (de) 2008-07-02 2011-03-23 Athenix Corporation Axmi-115, axmi-113, axmi-005, axmi-163 und axmi-184: schädlingsbekämpfende vip3a-proteine aus bacillus thuringiensis und verfahren für ihre verwendung
MX2011006342A (es) 2008-12-15 2011-10-06 Athenix Corp Genes que codifican toxinas contra nematodos.
EP2361307B1 (de) 2008-12-22 2014-09-24 Athenix Corporation Pestizidgene aus Brevibacillus und Verfahren zu deren Verwendung
BRPI0923159A2 (pt) 2008-12-23 2018-08-28 Athenix Corp gene de delta-endotoxina axmi-150 e métodos para sua utilização.
HUE032178T2 (en) 2009-02-05 2017-09-28 Athenix Corp Variable Axmi-Rl delta-endotoxin genes and methods for their use
EP2957638B1 (de) 2009-02-27 2018-12-12 Athenix Corporation Pestizide proteine und verfahren zu deren verwendung
AR075799A1 (es) 2009-03-06 2011-04-27 Athenix Corp Metodos y composiciones para controlar plagas de plantas
CA2754845A1 (en) 2009-03-11 2010-12-09 Athenix Corporation Axmi-030 insecticidal protein from bacillus thuringiensis and methods for use
ES2609332T3 (es) 2009-07-02 2017-04-19 Athenix Corporation Gen pesticida AXMI-205 y métodos para su uso
EA035563B1 (ru) 2009-07-31 2020-07-08 Басф Агрикалчерал Солюшнс Сид Юс Ллк Молекула рекомбинантной нуклеиновой кислоты, кодирующей полипептид с пестицидной активностью против чешуекрылых, жесткокрылых или нематодных вредителей, ее получение и применение
WO2011103248A2 (en) 2010-02-18 2011-08-25 Athenix Corp. AXMI221z, AXMI222z, AXMI223z, AXMI224z, AND AXMI225z DELTA-ENDOTOXIN GENES AND METHODS FOR THEIR USE
EP2536266A2 (de) 2010-02-18 2012-12-26 Athenix Corp. Axmi218-, axmi219-, axmi220-, axmi226-, axmi227-, axmi228-, axmi229-, axmi230- und axmi231-delta-endotoxin-gene sowie anwendungsverfahren dafür
US9321814B2 (en) 2011-03-30 2016-04-26 Athenix Corp. AXMI238 toxin gene and methods for its use
US9695440B2 (en) 2011-03-30 2017-07-04 Athenix Corp. AXMI232, AXMI233, and AXMI249 toxin genes and methods for their use
BR112013025665B8 (pt) 2011-04-05 2022-07-05 Athenix Corp Molécula de ácido nucleico recombinante, vetor, microrganismo transgênico, polipeptídeo recombinante e seu método de produção, composição, métodos para controlar ou exterminar uma população de pragas, para proteger uma planta de uma praga, e para aumentar o rendimento em uma planta
CN103597082B (zh) 2011-06-06 2017-09-15 拜尔作物科学公司 用于在预选位点修饰植物基因组的方法和手段
WO2014003769A1 (en) 2012-06-29 2014-01-03 Athenix Corp. Axmi277 nematode toxin and methods for its use
UY34227A (es) 2011-07-28 2013-02-28 Athenix Corp Acido nucleico recombinante que codifica el gen de la toxina axmi270, vectores, células, plantas y sus métodos de empleo
BR112014001978B8 (pt) 2011-07-28 2022-07-05 Athenix Corp Molécula de ácido nucleico recombinante, célula hospedeira, polipeptídeo, composição, métodos para controle, para proteção e para exterminação de uma população de pragas de lepidópteros ou coleópteros, método para produção de um polipeptídeo com atividade pesticida e método para aumentar a produtividade de uma planta
AR087366A1 (es) 2011-07-29 2014-03-19 Athenix Corp Gen plaguicida axmi 279 y sus metodos de empleo
EP2748323B1 (de) 2011-08-22 2019-05-01 BASF Agricultural Solutions Seed US LLC Verfahren und vorrichtung zur modifizierung eines pflanzengenoms
AR090274A1 (es) 2012-03-08 2014-10-29 Athenix Corp Gen de la toxina axmi335 de bacillus thuringiensis y sus metodos de empleo
CN104302663B (zh) 2012-03-08 2018-03-27 阿森尼克斯公司 AXMI345 δ‑内毒素基因及其使用方法
NZ727213A (en) 2012-03-09 2020-03-27 Vestaron Corp Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
US9758793B2 (en) 2012-08-30 2017-09-12 Athenix Corp. AXMI-234 and AXMI-235 delta-endotoxin genes and methods for their use
WO2014126986A1 (en) 2013-02-13 2014-08-21 Athenix Corp. Use of axmi184 for the control of rootworm insects
CN110172466A (zh) 2013-03-07 2019-08-27 巴斯夫农业解决方案种子美国有限责任公司 毒素基因及其使用方法
JP6463724B2 (ja) 2013-03-15 2019-02-06 バイエル クロップサイエンス エルピーBayer Cropscience Lp 構成ダイズプロモーター
UY35696A (es) 2013-08-09 2015-03-27 Athenix Corp ?molécula de adn recombinante que comprende al gen de toxina axmi440, vector, célula huésped, plantas, composiciones y métodos relacionados?.
US9862967B2 (en) 2013-08-09 2018-01-09 Athenix Corp. AXMI281 toxin gene and methods for its use
WO2015077525A1 (en) 2013-11-25 2015-05-28 Bayer Cropscience Lp Use of axmi-011 for the control of hemipteran insects
BR122021024287B1 (pt) 2013-12-09 2022-10-11 Athenix Corp Constructo, vetor, célula hospedeira bacteriana, polipeptídeo, composição, método de controle e exterminação de praga lepidóptera, método de proteção de planta e método de produção de polipeptídeo com atividade pesticida
US11021713B2 (en) 2016-05-25 2021-06-01 Cargill, Incorporated Engineered nucleases to generate deletion mutants in plants
MX2019004648A (es) 2016-10-21 2019-05-23 Vestaron Corp Peptidos escindibles y proteinas insecticidas y nematicidas que los comprenden.
MX2019005835A (es) 2016-11-23 2019-10-30 BASF Agricultural Solutions Seed US LLC Genes de toxinas axmi669 y axmi991 y metodos para su uso.
CN110225974B (zh) 2016-12-22 2024-03-29 巴斯夫农业种子解决方案美国有限责任公司 使用cry14来控制线虫害虫
AR110756A1 (es) 2017-01-18 2019-05-02 Bayer Cropscience Lp Uso de bp005 para el control de patógenos de planta
EP3571303A1 (de) 2017-01-18 2019-11-27 Basf Agricultural Solutions Seed Us Llc Bp005-toxin-gen und verfahren zu dessen verwendung
CN107303538B (zh) * 2017-05-23 2019-05-31 东南大学 一种生物分子分离装置及分离方法
AR114734A1 (es) 2018-09-14 2020-10-07 Vestaron Corp POLIPÉPTIDOS INSECTICIDAS MUTANTES Av3 Y MÉTODOS PARA PRODUCIRLOS Y USARLOS
AU2020204657A1 (en) 2019-01-04 2021-07-29 Pioneer Hi-Bred International, Inc. Engineered nucleases to generate mutations in plants
WO2021058659A1 (en) 2019-09-26 2021-04-01 Bayer Aktiengesellschaft Rnai-mediated pest control
PE20231212A1 (es) 2020-04-20 2023-08-17 Vestaron Corp Polipeptidos variantes proteoliticamente estables de u1-agatoxina-ta1b para el control de plagas
EP4142498A1 (de) 2020-05-01 2023-03-08 Vestaron Corporation Insektizide kombinationen
WO2022067214A2 (en) 2020-09-28 2022-03-31 Vestaron Corporation Mu-diguetoxin-dc1a variant polypeptides for pest control
BR112023019825A2 (pt) 2021-04-01 2024-01-16 Vestaron Corp Polipeptídeos mutantes av3 para controle de pragas
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9100915A1 *

Also Published As

Publication number Publication date
WO1991000915A1 (en) 1991-01-24
IE902504A1 (en) 1991-03-13
AU5950090A (en) 1991-02-06
AU642889B2 (en) 1993-11-04
IE61705B1 (en) 1994-11-30

Similar Documents

Publication Publication Date Title
US5240842A (en) Aerosol beam microinjector
AU642889B2 (en) Aerosol beam microinjector
US5036006A (en) Method for transporting substances into living cells and tissues and apparatus therefor
US5219746A (en) Ice-mediated introduction of substances into biological material
Morikawa et al. Capillary microinjection into protoplasts and intranuclear localization of injected materials
Sanford et al. Delivery of substances into cells and tissues using a particle bombardment process
US5100792A (en) Method for transporting substances into living cells and tissues
US7132289B2 (en) Method for introducing foreign matters into living cells
US7067716B2 (en) Methods and compositions for the introduction of molecules into cells
Aragao et al. Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device
WO1992001802A1 (en) Methods and apparatus for ice-medicated introduction of substances into biological material in a non-lethal manner
WO2007132891A1 (ja) 物質の細胞内導入法
JP2004511247A (ja) バイオリスティック装置
KR950014922B1 (ko) 물질을 생물학적 세포에 삽입시키기 위한 방법 및 장치
CA2062178A1 (en) Aerosol beam microinjector
Doležel et al. Flow analysis and sorting of plant chromosomes
JP3780333B2 (ja) 外来性遺伝物質又は生理活性物質を細胞内へ導入する新規な方法
US7314969B2 (en) Methods and compositions for the introduction of molecules into cells
Dhir et al. Optimization and transformation of Arundo donax L. using particle bombardment
Petolino Direct DNA delivery into intact cells and tissues
Horikawa et al. Transformants through pollination of mature maize (Zea mays L.) pollen delivered bar gene by particle gun
Hughes et al. Ultrastructure of nuclei isolated from plant protoplasts
CN106318897A (zh) 一种植物单染色体的激光显微切割分离方法
CA2429272C (en) Methods and compositions for the introduction of molecules into cells
Petolino Dow AgroSciences, Indianapolis, Indiana

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19920113

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB IT LI LU NL SE

17Q First examination report despatched

Effective date: 19940331

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19950504