CN110172466A - 毒素基因及其使用方法 - Google Patents

毒素基因及其使用方法 Download PDF

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CN110172466A
CN110172466A CN201910427962.8A CN201910427962A CN110172466A CN 110172466 A CN110172466 A CN 110172466A CN 201910427962 A CN201910427962 A CN 201910427962A CN 110172466 A CN110172466 A CN 110172466A
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R.塞耶
K.罗伯茨
K.桑普森
D.莱蒂嫩
C.彼得斯
L.马加尔黑斯
E.邓恩
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Abstract

在此提供了用于赋予细菌、植物、植物细胞、组织以及种子杀有害生物活性的组合物和方法。提供了包括针对毒素多肽的编码序列的组合物。这些编码序列可以用在DNA构建体或表达盒中，以便用于在植物和细菌中转化和表达。组合物还包括转化的细菌、植物、植物细胞、组织、以及种子。具体地说，提供了分离的毒素核酸分子。另外，涵盖了与这些多核苷酸相应的氨基酸序列、以及与这些氨基酸序列特异性结合的抗体。具体地说，本发明提供了分离的核酸分子，这些核酸分子包含编码SEQ ID NO：21‑74中所示的氨基酸序列的核苷酸序列、或在SEQ ID NO：1‑20中列出的核苷酸序列、以及它们的变体和片段。

Description

毒素基因及其使用方法
本发明是基于申请日为2014年3月6日，申请号为“201480012646.X”(国际申请号为PCT/US2014/021021)，发明名称为“毒素基因及其使用方法”的专利申请的分案申请。
相关申请的交叉引用
本专利申请要求于2013年3月7日提交的美国临时申请序列号61/774,110和各自于2013年3月8日提交的美国临时申请序列号61/774,627、61/774,629、61/774,635、61/774,638、61/774,642、61/774,645、61/774,647、61/774,650、61/774,655、以及61/774,659的权益，将其内容通过引用以其全文结合在此。
对以电子方式提交的序列表的引用
所述序列表的官方副本经由EFS-Web作为ASCII格式序列表以电子方式提交，文件名为“APA13-6008US01_SEQLIST.txt”，创建于2014年3月5日，并且具有411千字节大小，并且与本说明书同时提交。包含在这一ASCII格式文件中的序列表是本说明书的一部分并且通过引用以其全部内容结合在此。
发明领域
本发明涉及分子生物学领域。提供了编码杀有害生物蛋白的新颖的基因。这些蛋白和编码它们的核酸序列在制备杀有害生物配制品中以及在生产转基因抗有害生物植物中是有用的。
发明背素
苏云金芽孢杆菌是形成革兰氏阳性孢子的土壤细菌，其特征在于它具有产生结晶内含物的能力，这些结晶内含物对某些目和物种的昆虫是具有特异毒性的，但是对植物以及其他非靶向的生物是无害的。因此，包括苏云金芽孢杆菌菌株或它们的杀昆虫蛋白的组合物可用作环境上可接受的杀昆虫剂以用于控制农业昆虫有害生物或者各种人或动物疾病的昆虫媒介。
来自苏云金芽孢杆菌的晶体(Cry)蛋白(δ-内毒素)具有主要针对鳞翅目的、半翅目的、双翅目的、以及鞘翅目的幼虫的有效的杀昆虫活性。这些蛋白还已经显示针对膜翅目、同翅目、虱目、食毛目、以及蜱螨亚纲有害生物目(Acari pest order)、连同其他无脊椎动物目(如线虫动物门、扁形动物门、以及原生动物门肉鞭毛虫亚门)的活性(费特逊(Feitelson)(1993)苏云金芽孢杆菌家族树(The Bacillus Thuringiensis familytree)，先进的工程杀有害生物剂(Advanced Engineered Pesticides)，马塞尔穧德克尔格式(Marcel Dekker,Inc.)，纽约，纽约州)。这些蛋白最初主要基于它们的杀昆虫活性而分类为CryI至CryV。主要类别是鳞翅目特异性的(I)、鳞翅目和双翅目特异性的(II)、鞘翅目特异性的(III)、双翅目特异性的(IV)以及线虫特异性的(V)和(VI)。这些蛋白进一步分类成亚家族；在每个家族内更高度相关的蛋白被指定了分组字母例如Cry1A、Cry1B、Cry1C等等。在每个分组内甚至更加密切相关的蛋白被给予诸如Cry1C1、Cry1C2等的名称。
基于氨基酸序列同源性而不是昆虫靶标特异性描述了Cry基因的命名法(克里克莫尔(Crickmore)等人(1998)微生物学与分子生物学评论(Microbiol.Mol.Biol.Rev.)62：807-813)。在此分类中，每种毒素被指定了独特的名称，其中包括第一级(primary rank)(阿拉伯数字)、第二级(secondary rank)(大写字母)、第三级(tertiary rank)(小写字母)、以及第四级(quaternary rank)(另一个阿拉伯数字)。在第一级中，罗马数字已被换成阿拉伯数字。带有小于45％的序列一致性的蛋白具有不同的第一级，并且对于第二和第三级的标准对应地是78％和95％。
晶体蛋白不展示杀昆虫活性，直至其被摄取并溶解在昆虫中肠之中。被摄取的原毒素在昆虫消化道中被蛋白酶水解成活性毒性分子。(霍弗特和怀特利(Whiteley)(1989)生物学评论(Microbiol.Rev.)53：242-255)。这种毒素与靶标幼虫的中肠中的顶端刷状缘受体结合并插入顶端膜中，产生离子通道或孔，从而导致幼虫死亡。
δ-内毒素通常具有五个保守的序列结构域和三个保守的结构性结构域(参见，例如戴马格德(de Maagd)等人(2001)遗传学趋势(Trends Genetics)17：193-199)。第一个保守的结构性结构域由7个α螺旋组成并且参与膜插入和孔形成。结构域II由排列成希腊钥匙构型的三个β片层组成并且结构域III由“果冻卷”结构中的两个反向平行的β片层组成(戴马格德(de Maagd)等人，2001，同上)。结构域II和III参与受体识别和结合，并且因此被认为是毒素特异性的决定簇。
除了δ-内毒素之外，存在若干其他已知类别的杀有害生物蛋白毒素。这些VIP1/VIP2毒素(参见例如美国专利5,770,696)是二元杀有害生物毒素，它们通过如下机制对昆虫表现强活性，该机制被认为涉及受体介导的胞吞随后细胞毒化，类似于其他二元(“A/B”)毒素的作用方式。A/B毒素，例如VIP、C2、CDT、CST，或炭疽芽孢杆菌水肿和致命毒素，最初通过“B”组分(作为单体)的特异的受体介导的结合与靶标细胞相互作用。然后这些单体形成同七聚物(homoheptamer)。然后该“B”七聚物-受体复合物充当对接平台，随后该平台约束并允许酶的一种或多种“A”组分通过受体介导的胞吞而易位到细胞溶质中。一旦到细胞的细胞溶质内部，“A”组分通过例如G-肌动蛋白的ADP-核糖基化或增加环状AMP(cAMP)的细胞内水平而抑制正常细胞功能。参见巴斯(Barth)等人(2004)微生物学与分子生物学评论(Microbiol Mol Biol Rev)68：373-402。
大量使用基于苏云金芽孢杆菌的杀昆虫剂已经引起菱形斑蛾——小菜蛾的田间种群的抗性(费亥(Ferré)和范里(Van Rie)(2002)昆虫学年评(Annu.Rev.Entomol.)47：501-533)。最常见的抗性机制是该毒素与其一种或多种特异性中肠受体的结合的减少。这还可以赋予针对共享相同受体的其他毒素的交叉抗性(费亥(Ferré)和范里(Van Rie)(2002))。
由于昆虫可能带来破坏、以及通过控制昆虫有害生物在产量方面的改进，对于发现新形式的杀有害生物毒素存在着不断的需要。
发明概述
在此提供了用于赋予细菌、植物、植物细胞、组织以及种子杀有害生物活性的组合物和方法。组合物包括针对杀有害生物的和杀昆虫的多肽的核酸分子编码序列、包括那些核酸分子的载体、以及包括这些载体的宿主细胞。组合物还包括这些杀有害生物多肽序列以及针对那些多肽的抗体。这些核苷酸序列可以用在DNA构建体或表达盒中，以用于在多种生物(包括微生物和植物)中进行转化和表达。这些核苷酸或氨基酸序列可以是合成序列，这些合成序列已经被设计为用于在生物中表达，该生物包括但不局限于：微生物或植物。组合物还包括含有本发明的核苷酸序列的细菌、植物、植物细胞、组织、以及种子。
具体地说，提供了编码杀有害生物蛋白的分离的或重组的核酸分子。另外，涵盖了与这些杀有害生物蛋白相应的氨基酸序列。具体地，本发明提供了分离或重组的核酸分子，该核酸分子包括编码SEQ ID NO：21-74中所示的氨基酸序列的核苷酸序列、或在SEQ IDNO：1-20中列出的核苷酸序列、以及它们的生物活性变体和片段。还涵盖了与本发明的核苷酸序列互补的核苷酸序列、或与本发明的序列或其互补物杂交的核苷酸序列。另外还提供了包括本发明的核苷酸序列或者编码本发明的氨基酸序列的核苷酸序列以及它们的生物活性变体和片段的载体、宿主细胞、植物以及种子。
提供了用于产生本发明的多肽的方法，以及用于使用这些多肽来控制或杀死鳞翅目、半翅目、鞘翅目、线虫、或双翅目有害生物的方法。还包括用于检测在样品中的本发明的核酸和多肽的方法和试剂盒。
本发明的组合物和方法用于产生具有增强的有害生物抗性或耐受性的生物。这些生物以及包括这些生物的组合物对于农业目的是所希望的。本发明的组合物还用于产生具有杀有害生物活性的改变的或改进的蛋白、或检测在产物或生物中的杀有害生物蛋白或核酸的存在。
详细说明
本发明描绘了用于调节生物(特别是植物或植物细胞)中的有害生物抗性或耐受性的组合物和方法。“抗性”是指该有害生物(例如，昆虫)一旦摄取或以其他方式接触本发明的多肽类时就被杀死。“耐受性”是指有害生物的运动、摄食、繁殖、或其他功能的损害或降低。这些方法涉及用编码本发明的杀有害生物蛋白的核苷酸序列来转化生物。具体地说，本发明的核苷酸序列用于制备具有杀有害生物活性的植物和微生物。因此，提供了转化的细菌、植物、植物细胞、植物组织以及种子。组合物是杆菌或其他物种的杀有害生物核酸和蛋白。这些序列用于随后转化到感兴趣的生物中的表达载体的构建，作为用于其他同源(或部分同源的)基因的分离的探针，以及用于通过本领域中已知的方法(如，结构域交换或DNA改组)来产生改变的杀有害生物蛋白，例如内毒素的Cry1、Cry2以及Cry9家族成员。这些蛋白用于控制或杀死鳞翅目、半翅目、鞘翅目、双翅目、以及线虫有害生物种群、以及用于生产具有杀有害生物活性的组合物。
“杀有害生物毒素”或“杀有害生物蛋白”是指毒素，该毒素具有针对一种或多种有害生物的毒性，包括但不局限于：鳞翅目、双翅目、以及鞘翅目、或线虫动物门成员、或者与这种蛋白具有同源性的蛋白。已经从生物中分离出杀有害生物蛋白，这些生物包括例如，芽孢杆菌属物种、双酶梭菌、以及日本甲虫类芽孢杆菌(Paenibacillus popilliae)。杀有害生物蛋白包括从在此披露的全长核苷酸序列中推导出的氨基酸序列、以及比这些全长序列更短的氨基酸序列(或者由于使用了可替代的下游起始位点、或者由于产生较短的具有杀有害生物活性的蛋白的加工过程)。加工可以在表达该蛋白的生物内发生，或在该有害生物摄取该蛋白之后发生。
杀有害生物蛋白类涵盖了δ-内毒素。δ-内毒素包括被标识为cry1至cry72、cyt1和cyt2、以及Cyt样毒素的蛋白。目前存在着超过250种己知的δ-内毒素种类，它们具有宽范围的特异性和毒性。关于扩展性列表，参见克里克莫尔(Crickmore)等人(1998)，微生物学与分子生物学评论(Microbiol.Mol.Biol.Rev.)62：807-813，并且对于定期更新，参见克里克莫尔(Crickmore)等人(2003)“苏云金芽孢杆菌毒素命名法(Bacillus thuringiensistoxin nomenclature)”，在www.biols.susx.ac.uk/Home/Neil_Crickmore/Bt/index处。
因此，在此提供了新颖的分离或重组的核苷酸序列，这些序列赋予了杀有害生物活性。这些核苷酸序列编码与已知的δ-内毒素或二元毒素具有同源性的多肽。还提供了杀有害生物蛋白的氨基酸序列。由这种基因的翻译而产生的蛋白允许细胞控制或杀死摄取了该蛋白的有害生物。
核酸分子及其变体和片段
本发明的一个方面涉及分离的或重组的核酸分子，这些核酸分子包括编码杀有害生物蛋白和多肽或它们的生物活性部分的核苷酸序列；以及足以用作杂交探针来鉴定编码具有序列同源性的区域的蛋白的核酸分子的核酸分子。在此还涵盖了能够在如在此的其他部分所定义的严格条件下与本发明的核苷酸序列杂交的核苷酸序列。如本文所使用的，术语“核酸分子”旨在包括DNA分子(例如，重组DNA、cDNA或基因组DNA)和RNA分子(例如，mRNA)以及使用核苷酸类似物而产生的DNA或RNA的类似物。该核酸分子可以是单链的或双链的，但优选地是双链DNA。术语“重组”包括多核苷酸或多肽，该多核苷酸或多肽已相对于天然多核苷酸或多肽被操纵，使得该多核苷酸或多肽不同(例如，在化学组成或结构上)于自然界中存在的那样。在另一个实施例中，“重组”多核苷酸不含天然存在于衍生该多核苷酸的生物体的基因组DNA中的内部序列(即内含子)。这种多核苷酸的典型实例是所谓的互补DNA(cDNA)。
分离的或重组的核酸(或DNA)被用在此处是指如下的核酸(或DNA)，该核酸(或DNA)不再存在于它的自然环境中(例如，在体外或在重组细菌或植物宿主细胞中)。在一些实施例中，分离的或重组的核酸是不含有在衍生出该核酸的生物的基因组DNA中天然地位于该核酸侧翼的序列(即，位于该核酸的5'和3'末端的序列)(优选编码蛋白的序列)。出于本发明的目的，“分离的”当用于指核酸分子时不包括分离的染色体。例如，在不同的实施例中，编码分离的δ内毒素的核酸分子可包括小于约5kb、4kb、3kb、2kb、1kb、0.5kb、或0.1kb的核苷酸序列，这些核苷酸序列在衍生出该核酸的细胞的基因组DNA中天然地位于该核酸分子侧翼。在不同实施例中，基本上不含细胞材料的δ-内毒素蛋白包括具有低于约30％、20％、10％或5％(按干重计)的非δ-内毒素蛋白(在此也称为“污染性蛋白”)的蛋白制剂。
编码本发明的蛋白的核苷酸序列包括SEQ ID NO：1-20中列出的序列及其变体、片段和互补物。“互补物”是指核苷酸序列，该核苷酸序列与给定的核苷酸序列足够地互补，这样使得它可以与该给定的核苷酸序列杂交以便由此形成稳定的双链体。对于由这些核苷酸序列所编码的杀有害生物蛋白的相应氨基酸序列在SEQ ID NO：21-74中列出。
本发明还涵盖了以下核酸分子，这些核酸分子是这些编码杀有害生物蛋白的核苷酸序列的片段。“片段”是指编码杀有害生物蛋白的核苷酸序列的部分。核苷酸序列的片段可以编码杀有害生物蛋白的生物活性部分，或者它是使用以下披露的方法可以用作杂交探针或PCR引物的片段。作为编码杀有害生物蛋白的核苷酸序列的片段的核酸分子包括至少约50、100、200、300、400、500、600、700、800、900、1000、1100、1200、1300、1350、1400个连续核苷酸，或达到在此所披露的全长的编码杀有害生物蛋白的核苷酸序列中存在的核苷酸的数目，这取决于所预期的用途。“相邻的”核苷酸旨在指彼此紧邻的核苷酸残基。本发明的核苷酸序列的片段将编码保留杀有害生物蛋白的生物活性、并且因此保留杀有害生物活性的蛋白片段。因此，还涵盖了在此披露的多肽的生物活性片段。“保留活性”是指片段将具有杀有害生物蛋白的至少约30％、至少约50％、至少约70％、80％、90％、95％或更高的杀有害生物活性。在一个实施例中，杀有害生物活性是杀鞘翅目活性。在另一个实施例中，杀有害生物活性是杀鳞翅目活性。在另一个实施例中，杀有害生物活性是杀线虫活性。在另一个实施例中，杀有害生物活性是杀双翅目活性。在另一个实施例中，杀有害生物活性是杀半翅目活性。用于测量杀有害生物活性的方法在本领域是熟知的。参见例如，恰普拉(Czapla)和朗(Lang)(1990)经济昆虫学杂志(J.Econ.Entomol.)83：2480-2485；安德鲁斯(Andrews)等人(1988)生物化学杂志(Biochem.J.)252：199-206；马罗内(Marrone)等人(1985)经济昆虫学杂志(J.of Economic Entomology)78：290-293；以及美国专利号5,743,477，将它们全部通过引用以其全文结合在此。
编码杀有害生物蛋白的核苷酸序列的片段(该序列编码本发明的蛋白的生物活性部分)将编码至少约15、25、30、50、75、100、125、150、175、200、250、300、350、400、450、500、550、600、650、700、750、800、850、900、950、1000、1050、1100、1150、1200个相邻氨基酸，或高达存在于本发明的全长杀有害生物蛋白中的氨基酸的全部数量。在一些实施例中，片段是蛋白酶切片段。例如，蛋白酶切片段可以相对于SEQ ID NO：21-74具有至少约100个氨基酸、约120个、约130个、约140个、约150个、或约160个氨基酸的N末端或C末端截短。在一些实施例中，在此所涵盖的这些片段是由例如通过蛋白酶解或通过在编码序列中插入终止密码子来除去C末端结晶结构域而产生。参见，例如，在SEQ ID NO：25、26、39-45、49-51、58、63-64、66、68、以及72-74中列出的截短氨基酸序列。应当理解的是，该截短部位可以随着由SEQ IDNO：25、26、39-45、49-51、58、63-64、66、68、或72-74的末端表示的截短部位(相比于相应全长序列)的任一侧上的1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、或更多个氨基酸变化。
本发明的优选杀有害生物蛋白由与SEQ ID NO：1-20的核苷酸序列足够一致的核苷酸序列编码，或者这些杀有害生物蛋白与SEQ ID NO：21-74中所列的氨基酸序列足够一致。“足够地一致的”是指氨基酸或核苷酸的序列，使用在本文中所说明的比对程序之一使用标准的参数，与参比序列相比，该氨基酸或核苷酸的序列具有至少约60％或65％的序列一致性，约70％或75％的序列一致性，约80％或85％的序列一致性，约90％、91％、92％、93％、94％、95％、96％、97％、98％、或99％或更高的序列一致性。本领域的普通技术人员将会认识到，可以对这些值进行适当地调整以便通过考虑密码子简并性、氨基酸相似性、阅读框定位、等等来确定由两个核苷酸序列编码的蛋白的相应一致性。
为了确定两个氨基酸序列或两个核酸的一致性百分比，出于最佳比较目的而比对这些序列。这两个序列之间的一致性百分比是由这些序列共有的相同位置的数目的函数(即，一致性百分比＝相同位置的数目/位置(例如，重叠位置)的总数目x 100)。在一个实施例中，这两个序列是相同长度的。在另一个实施例中，一致性百分比是贯穿参比序列的整体来计算的(即，在此所披露的如SEQ ID NO：1-74中任一项的序列)。在两个序列之间的一致性百分比可以使用与以下说明的那些相似的技术来确定，其中允许或不允许空位。在计算一致性百分比中，对典型地准确的匹配进行计数。空位(即，在其中残基存在于一个序列中但不存在于另一个序列中的比对中的位置)被认为是具有非一致性残基的位置。
两个序列之间的一致性百分比的确定可以通过使用数学算法来完成。用于比较两个序列的数学算法的非限制性实例是卡尔林(Karlin)和阿尔丘尔(Altschul)(1990)美国国家科学院院刊(Proc.Natl.Acad.Sci.USA)87：2264的算法，该算法在卡林和阿特休尔(1993)美国国家科学院院刊90：5873-5877中进行了修改。这样一种算法被结合到阿尔丘尔(Altschul)等人(1990)分子生物学杂志(J.Mol.Biol.)215：403的BLASTN和BLASTX程序中。BLAST核苷酸搜索可以用BLASTN程序(得分＝100、字长＝12)来进行，以获得与本发明的杀有害生物样核酸分子同源的核苷酸序列。BLAST蛋白搜索可以用BLASTX程序(得分＝50、字长＝3)来进行，以获得与本发明的杀有害生物蛋白分子同源的氨基酸序列。为了获得用于比较目的的空位比对，可以使用如阿尔丘尔等人(1997)核酸研究(Nucleic Acids Res.)25：3389中所描述的缺口BLAST(在BLAST 2.0中)。可替代地，可以使用PSI-Blast进行迭代搜索，该迭代搜索检测分子之间的远近关系。参见阿尔丘尔等人(1997)同上。当利用BLAST、空位BLAST和PSI-Blast程序时，可以使用对应的程序(例如，BLASTX和BLASTN)的默认参数。还可以通过检查，手动进行比对。
用于序列比较的数学算法的另一个非限制实例是ClustalW算法(希金斯(Higgins)等人(1994)核酸研究(Nucleic Acids Res.)22：4673-4680))。ClustalW比较了序列并且比对了氨基酸或DNA序列整体，并且因此可以提供有关整个氨基酸序列的序列保守性的数据。ClustalW算法用于几个可商购的DNA/氨基酸分析软件包，如Vector NTIProgram Suite的ALIGNX模块(英杰公司(Invitrogen Corporation)，卡尔斯巴德(Carlsbad)，加利福尼亚(CA))。用ClustalW进行氨基酸序列比对之后，可以估算出氨基酸一致性百分数。适用于ClustalW比对分析的软件程序的非限制性实例是GENEDOCTM。GENEDOCTM(卡尔尼古拉斯公司(Karl Nicholas))允许在多个蛋白之间评估氨基酸(或DNA)相似性和一致性。用于比较序列的数学算法的另一个非限制性实例是迈尔斯(Myers)和米勒(Miller)(1988)CABIOS 4：11-17的算法。这种算法被结合到ALIGN程序(版本2.0)中，该程序是GCG威斯康星遗传学软件包(Wisconsin Genetics Software Package)(版本10)(可商购自阿塞勒德公司(Accelrys,Inc.)，斯克兰顿路9685号(9685Scranton Rd.)，圣地亚哥(San Diego)，加利福尼亚州，美国)的一部分。当利用ALIGN程序来比较氨基酸序列时，可以使用PAM120权重残基表、空位长度罚分12、以及空位罚分4。
除非另外说明，GAP版本10(它使用尼德尔曼(Needleman)和翁施(Wunsch)(1970)分子生物学杂志(J.Mol.Biol.)48(3)：443-453的算法)将使用以下参数用于确定序列一致性或相似性：对于核苷酸序列的一致性百分比和相似性百分比，使用GAP权重50和长度权重3以及nwsgapdna.cmp评分矩阵；对于氨基酸序列的一致性百分比和相似性百分比，使用GAP权重8和长度权重2以及BLOSUM62评分程序。还可以使用等效程序。“等效程序”是指以下任何序列比较程序，该程序针对所研究的任何两个序列、当与由GAP版本10所产生的相应的比对相比时产生了比对，该比对具有相同的核苷酸残基匹配以及相同的序列一致性百分比。
本发明还涵盖了变体核酸分子。编码杀有害生物蛋白的核苷酸序列的“变体”包括编码在本文中所披露的杀有害生物蛋白、但由于遗传密码的简并而保守性地不同的那些序列，连同如以上所讨论的足够一致的那些序列。天然存在的等位基因变体可以使用所熟知的分子生物学技术来鉴定，例如像以下所概括的聚合酶链式反应(PCR)以及杂交技术。变体核苷酸序列还包括合成地衍生的核苷酸序列，这些核苷酸序列已经例如通过使用定点诱变而产生但它们仍编码在本发明中所披露的杀有害生物蛋白，如以下所讨论的。本发明所涵盖的变体蛋白是有生物活性的，即它们继续具有所希望的天然蛋白的生物活性，即杀有害生物活性。“保留活性”是指该片段将具有该天然蛋白的至少约30％、至少约50％、至少约70％、或至少约80％的杀有害生物活性。用于测量杀有害生物活性的方法在本领域是熟知的。参见例如，恰普拉(Czapla)和朗(Lang)(1990)经济昆虫学杂志(J.Econ.Entomol.)83：2480-2485；安德鲁斯(Andrews)等人(1988)生物化学杂志(Biochem.J.)252：199-206；马罗内(Marrone)等人(1985)经济昆虫学杂志(J.of Economic Entomology)78：290-293；以及美国专利号5,743,477，将它们全部通过引用以其全文结合在此。
熟练的技术人员将进一步意识到，可以通过突变本发明的核苷酸序列来引入变化，由此导致在这些编码的杀有害生物蛋白的氨基酸序列中的变化，而不改变这些蛋白的生物活性。因此，可以通过将一个或多个核苷酸置换、添加、或缺失引入到在此披露的相应核苷酸序列中而产生分离的核酸分子变体，使得将一个或多个氨基酸置换、添加、或缺失引入到编码的蛋白中。可以通过标准的技术(如定点诱变和PCR介导的诱变)来引入突变。这样的变体核苷酸序列也由本发明所涵盖。
例如，可以在一个或多个、预测的、非必需氨基酸残基处做出保守性氨基酸置换。“非必需”氨基酸残基是以下残基，该残基可以从杀有害生物蛋白的野生型序列中进行改变而不改变生物活性，而“必需”氨基酸残基是生物活性所要求的。“保守的氨基酸置换”是其中氨基酸残基被具有相似侧链的氨基酸残基替换的置换。在本领域中已经定义了具有类似侧链的氨基酸残基的家族。这些家族包括具有以下的氨基酸：碱性侧链(例如赖氨酸、精氨酸、组氨酸)，酸性侧链(例如天冬氨酸、谷氨酸)，不带电极性侧链(例如甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)，非极性侧链(例如丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)，β-支链的侧链(例如苏氨酸、缬氨酸、异亮氨酸)以及芳香族侧链(例如酪氨酸、苯丙氨酸、色氨酸、组氨酸)。
δ-内毒素通常具有五个保守的序列结构域和三个保守的结构性结构域(参见，例如戴马格德(de Maagd)等人(2001)遗传学趋势(Trends Genetics)17：193-199)。第一个保守的结构性结构域由7个α螺旋组成并且参与膜插入和孔形成。结构域II由排列成希腊钥匙构型的三个β片层组成并且结构域III由“果冻卷”结构中的两个反向平行的β片层组成(戴马格德(de Maagd)等人，2001，同上)。结构域II和III参与受体识别和结合，并且因此被认为是毒素特异性的决定簇。
氨基酸置换可以在保留功能的非保守性区域中做出。通常，此类置换并非针对保守的氨基酸残基、或针对在保守基序内的氨基酸残基而做出，其中此类残基是蛋白活性所必需的。保守的并且对于蛋白活性可能是必需的残基的实例包括例如以下残基，这些残基在与本发明的序列相似或相关的毒素的比对中所包括的所有蛋白之间是相同(例如，在同源蛋白的比对中是相同的残基)。保守的但可能允许保守性氨基酸置换、并且仍保留活性的残基的实例包括例如以下残基，这些残基在与本发明的序列相似或相关的毒素的比对中所包括的所有蛋白之间仅具有保守性置换(例如，在比对同源蛋白中所包括的所有蛋白之间仅具有保守性置换的残基)。然而，本领域的普通技术人员应当理解，功能性变体在保守的残基中可以具有少量的保守性或非保守性改变。
可替代地，可以通过沿着该编码序列的全部或部分随机地引入突变(如通过饱和诱变)来制备变体核苷酸序列，并且可以针对赋予杀有害生物活性的能力来筛选得到的突变体以鉴定保留活性的突变体。在诱变之后，可以重组表达编码的蛋白，并且可以使用标准的测定技术来测定蛋白的活性。
使用方法如PCR、杂交等可以鉴定相应的杀有害生物序列，此类序列与本发明的序列具有实质上一致性(例如，贯穿参比序列的整体，至少约70％、至少约75％、80％、85％、90％、95％或更高序列一致性)并且具有或赋予了杀有害生物活性。参见，例如萨姆布鲁克(Sambrook)和拉塞尔(Russell)(2001)分子克隆：实验室手册(Molecular Cloning:ALaboratory Manual.)(冷泉港实验室出版社(Cold Spring Harbor Laboratory Press)，冷泉港(Cold Spring Harbor)，纽约)以及英尼斯(Innis)等人(1990)PCR方案：方法和应用指导(PCR Protocols:A Guide to Methods and Applications)(学术出版社(AcademicPress)，纽约)。
在杂交方法中，该杀有害生物核苷酸序列的全部或部分可以用于筛选cDNA或基因组文库。用于构建此类cDNA和基因组文库的方法在本领域内通常是已知的，并且披露于萨姆布鲁克和拉塞尔，2001，同上。所谓的杂交探针可以是基因组DNA片段、cDNA片段、RNA片段或其他寡核苷酸，并且可以用可检测基团(如32P)或任何其他可检测标记物(如其他放射性同位素、荧光化合物、酶或酶辅因子)进行标记。可以基于在此披露的已知的编码杀有害生物蛋白的核苷酸序列通过标记合成寡核苷酸来制备用于杂交的探针。可以另外地使用简并引物，这些引物是基于在该核苷酸序列或编码的氨基酸序列中的保守性核苷酸或氨基酸残基而设计的。这种探针典型地包括以下核苷酸序列的区域，这些核苷酸序列区域在严格条件下与本发明的编码杀有害生物蛋白的核苷酸序列或它的片段或变体的至少约12个、至少约25个、至少约50、75、100、125、150、175、或200个连续核苷酸进行杂交。用于制备用于杂交的探针的方法通常是本领域已知的，并且披露于萨姆布鲁克和拉塞尔，2001(同上)中，该文献通过引用结合在此。
例如，在此披露的完整的杀有害生物序列、或它的一个或多个部分可以用作探针，该探针能够与相应的杀有害生物蛋白样序列以及信使RNA特异性地杂交。为了实现在不同的条件下的特异性杂交，这样的探针包括以下序列，这些序列是独特的并且长度优选为至少约10个核苷酸、或长度为至少约20个核苷酸。这样的探针可以用于通过PCR从选择的生物或样品中扩增对应的杀有害生物序列。这种技术可以用于从所希望的生物中分离另外的编码序列，或用作诊断性测定以便确定在生物中的编码序列的存在。杂交技术包括杂交筛选平板接种的DNA文库(噬斑或菌落；参见，例如萨姆布鲁克等人(1989)分子克隆：实验室手册(第二版，冷泉港实验室出版社，冷泉港，纽约))。
因此，本发明涵盖了用于杂交的探针以及能够与本发明的核苷酸序列的全部或部分(例如，至少约300个核苷酸、至少约400个、至少约500、1000、1200、1500、2000、2500、3000、3500个核苷酸、或者达到在此披露的核苷酸序列的全长)杂交的核苷酸序列。这样的序列的杂交可以在严格条件下进行。“严格条件”或“严格杂交条件”是指以下条件，在这些条件下与其他序列相比探针将与它的靶标序列杂交达到可检测地更大的程度(例如，超过背景至少2倍)。严格条件是序列依赖性的并且在不同的情形下将会不同。通过控制杂交和/或洗涤条件的严格性，可以鉴定出与该探针100％互补的靶标序列(同源探测)。可替代地，可以调整严格条件以允许序列中的一些错配，这样使得检测到更低程度的相似性(异源探测)。通常，探针的长度为小于约1000个核苷酸，优选地长度小于500个核苷酸。
典型地，严格条件将是以下条件，在这些条件下该盐浓度在pH 7.0-8.3下是小于约1.5M Na离子，典型地大约0.01至1.0M Na离子浓度(或其他盐类)，并且温度对于短探针(例如，10至50个核苷酸)为至少约30℃，而对于长探针(例如，超过50个核苷酸)为至少约60℃。还可以通过加入去稳定剂(如甲酰胺)实现严格条件。示例的低严格条件包括在37℃下用30％-35％甲酰胺、1M NaCl、1％SDS(十二烷基硫酸纳)的缓冲溶液进行杂交，并且在50℃至55℃下在1X至2X SSC(20X SSC＝3.0M NaCl/0.3M柠檬酸三钠)中进行洗涤。示例的中严格条件包括在37℃下在40％-45％甲酰胺、1.0M NaCl、1％SDS中进行杂交，并且在55℃至60℃下在0.5X至1X SSC中进行洗涤。示例的高严格条件包括在37℃下在50％甲酰胺、1MNaCl、1％SDS中进行杂交，并且在60℃至65℃下在0.1X SSC中进行洗涤。任选地，洗涤缓冲液可以包括大约0.1％至大约1％的SDS。杂交的持续时间通常是小于约24小时，通常为约4至约12小时。
特异性典型地取决于杂交后洗涤的功能，关键因素是最终洗涤溶液的离子强度以及温度。对于DNA-DNA杂合体，可以根据迈因科思(Meinkoth)和瓦尔(Wahl)(1984)分析生物化学(Anal.Biochem.)138：267-284的方程估计Tm：Tm＝81.5℃+16.6(log M)+0.41(％GC)-0.61(％form)-500/L；其中M是一价阳离子的摩尔浓度，％GC是DNA中鸟苷和胞嘧啶核苷酸的百分比，％form是杂交溶液中甲酰胺的百分比，并且L是杂合体的碱基对长度。Tm是以下温度，在该温度下互补的靶标序列的50％与完全匹配的探针进行杂交(在限定的离子强度和pH下)。对于每1％的错配，Tm减少大约1℃；因此，可以调整Tm、杂交、和/或洗涤条件，以便与具有所希望的一致性的序列进行杂交。例如，如果寻找具有>90％一致性的序列，则Tm可以减少10℃。总体上，严格条件被选择为在限定的离子强度和pH下比针对该特定序列及其互补物的热解链点(Tm)低大约5℃。然而，极端严格条件可以利用在低于该热解链点(Tm)1℃、2℃、3℃或4℃下的杂交和/或洗涤；中等严格条件可以利用在低于该热解链点(Tm)6℃、7℃、8℃、9℃、或10℃下的杂交和/或洗涤；低严格条件可以利用在低于该热解链点(Tm)11℃、12℃、13℃、14℃、15℃或20℃下的杂交和/或洗涤。使用该方程、杂交和洗涤组成以及所希望的Tm，本领域普通技术人员应理解，杂交的和/或洗涤溶液的严格性的变化固有地得到了描述。如果所希望的错误匹配程度导致小于45℃(水性溶液)或32℃(甲酰胺溶液)的Tm，则优选增加SSC浓度，这样使得可以使用更高的温度。对核酸杂交的广泛指导见于以下文献：泰森(Tijssen)(1993)生物化学和分子生物学中的实验室技术-使用核酸探针杂交(Laboratory Techniques in Biochemistry and Molecular Biology-Hybridizationwith Nucleic Acid Probes)，第I部分，第2章(爱思唯尔(Elsevier)，纽约)；以及奥苏贝尔(Ausubel)等人，编著(1995)现代分子生物学方法(Current Protocols in MolecularBiology)，第2章(格林出版和威利科学公司(Greene Publishing and Wiley-Interscience)，纽约)。参见萨姆布鲁克等人(1989)分子克隆：实验室手册(第2版，冷泉港实验室出版社，冷泉港，纽约)。
分离的蛋白及其变体和片段
杀有害生物蛋白也涵盖于本发明之内。“杀有害生物蛋白”是指具有SEQ ID NO：21-74中列出的氨基酸序列的蛋白。还提供了它的片段、生物活性部分以及变体，并且它们可以用于实践本发明的方法。“重组蛋白”或“重组多肽”是用于指如下的蛋白，该蛋白不再存在于它的自然环境中并且已相对于天然蛋白被操纵，使得该重组蛋白或重组多肽不同(例如，在化学组成或结构上)于自然界中存在的那样。
“片段”或“生物学活性部分”包括以下多肽片段，这些多肽片段包括与在SEQ IDNO：21-74中列出的氨基酸序列足够地一致的氨基酸序列的多肽片段，并且它们展示出杀有害生物活性。杀有害生物蛋白的生物活性部分可以是以下多肽，该多肽的长度例如为10、25、50、100、150、200、250、300、350、400、450、500、550、600、650、700、750、800、850、900、950、1000、1050、1100、1150、1200、或更多个氨基酸。可以通过重组技术制备这样的生物活性部分，并且对它们的杀有害生物活性进行评估。用于测量杀有害生物活性的方法在本领域是熟知的。参见例如，恰普拉(Czapla)和朗(Lang)(1990)经济昆虫学杂志(J.Econ.Entomol.)83：2480-2485；安德鲁斯(Andrews)等人(1988)生物化学杂志(Biochem.J.)252：199-206；马罗内(Marrone)等人(1985)经济昆虫学杂志(J.of EconomicEntomology)78：290-293；以及美国专利号5,743,477，将它们全部通过引用以其全文结合在此。如本文中使用的，片段包括SEQ ID NO：21-74的至少8个连续的氨基酸。然而，本发明涵盖了其他片段，如在长度上大于约10、20、30、50、100、150、200、250、300、350、400、450、500、550、600、650、700、750、800、850、900、950、1000、1050、1100、1150、1200、或更多个氨基酸的蛋白中的任何片段。
“变体”是指具有以下氨基酸序列的蛋白或多肽，该氨基酸序列与SEQ ID NO：21-74的任一项的氨基酸序列具有至少约60％、65％，约70％、75％，约80％、85％，约90％、91％、92％、93％、94％、95％、96％、97％、98％或99％一致性。变体还包括由与SEQ ID NO:1-20的核酸分子或其互补物在严格条件下杂交的核酸分子编码的多肽。变体包括由于诱变而在氨基酸序列方面不同的多肽。由本发明所涵盖的变体蛋白是生物活性的，即它们继续具有所希望的天然蛋白的生物活性，即保留了杀有害生物活性。在一些实施例中，这些变体相对于天然蛋白具有改进的活性。用于测量杀有害生物活性的方法在本领域是熟知的。参见例如，恰普拉(Czapla)和朗(Lang)(1990)经济昆虫学杂志(J.Econ.Entomol.)83：2480-2485；安德鲁斯(Andrews)等人(1988)生物化学杂志(Biochem.J.)252：199-206；马罗内(Marrone)等人(1985)经济昆虫学杂志(J.of Economic Entomology)78：290-293；以及美国专利号5,743,477，将它们全部通过引用以其全文结合在此。
细菌基因(如本发明的axmi基因)在开放阅读框的起始附近常常具有多个甲硫氨酸起始密码子。经常，在这些起始密码子中的一个或多个处的翻译起始将导致功能蛋白的产生。这些起始密码子可以包括ATG密码子。然而，细菌(如，芽孢杆菌属)还将密码子GTG识别为起始密码子，并且在GTG密码子处起始翻译的蛋白在第一个氨基酸上包含甲硫氨酸。在少数情况下，细菌系统中的翻译可以在TTG密码子处启动，尽管在这个事件下TTG编码甲硫氨酸。此外，常常不首先确定这些密码子中的哪一些在细菌中自然地使用。因此，应当理解，使用这些可替代的甲硫氨酸密码子之一也可能导致杀有害生物蛋白的产生。这些杀有害生物蛋白涵盖于本发明之中，并且可以在本发明的这些方法中使用。应当理解的是当在植物中表达时有必要将替代起始密码子改变为ATG以用于正确的翻译。
在本发明的不同实施例中，杀有害生物蛋白包括从在此披露的全长核苷酸序列中推导出的氨基酸序列、以及由于使用替代的下游起始位点而比这些全长序列更短的氨基酸序列。因此，本发明的核苷酸序列和/或包括本发明的核苷酸序列的载体、宿主细胞以及植物(以及制备和使用本发明的核苷酸序列的方法)可以包括编码与表1中提及的氨基酸序列相应的氨基酸序列的核苷酸序列。
还涵盖了针对本发明的多肽、或它们的变体或片段的抗体。用于产生抗体的方法在本领域是熟知的(参见，例如哈洛(Harlow)和拉内(Lane)(1988)抗体：实验室手册(Antibodies:A Laboratory Manual)，冷泉港实验室，冷泉港，纽约；美国专利号4,196,265)。
因此，本发明一方面涉及与本发明的蛋白或肽分子中的一种或多种以及它们的同源物、融合物或片段特异性结合的抗体、单链抗原结合分子、或其他蛋白。在特别优选的实施例中，该抗体与具有SEQ ID NO：21-74中列出的氨基酸序列的蛋白或其片段特异性地结合。在另一个实施例中，该抗体与包含选自SEQ ID NO：21-74中列出的氨基酸序列的氨基酸序列的融合蛋白或其片段特异性地结合。
本发明的抗体可以用于定量地或定性地检测本发明的蛋白或肽分子、或者检测这些蛋白的翻译后修饰。如在此所使用的，如果抗体或肽与本发明的蛋白或肽分子的结合不被非相关分子的存在竞争性地抑制，则将这种结合称为“特异性地结合”。
本发明的抗体可以被包含在试剂盒中，该试剂盒用于检测本发明的蛋白或肽分子。本发明进一步包括检测本发明的蛋白或肽分子(特别是由SEQ ID NO：21-74中列出的氨基酸序列编码的蛋白，包括能够特异性结合到本发明的抗体的变体或片段)的方法，该方法包括使样品与本发明的抗体接触，并且确定该样品是否含有本发明的蛋白或肽分子。用于利用抗体来检测感兴趣的蛋白或肽的方法是本领域中已知的。
改变或改进的变体
应当认识到，可以通过不同的方法来改变杀有害生物蛋白的DNA序列，并且这些改变可以导致编码以下蛋白的DNA序列，这些蛋白具有不同于由本发明的杀有害生物蛋白编码的氨基酸序列的那些。这种蛋白可以按照不同的方式进行改变，包括SEQ ID NO：21-74的一个或多个氨基酸的氨基酸置换、缺失、截短、以及插入，包括达到约2、约3、约4、约5、约6、约7、约8、约9、约10、约15、约20、约25、约30、约35、约40、约45、约50、约55、约60、约65、约70、约75、约80、约85、约90、约100、约105、约110、约115、约120、约125、约130、约135、约140、约145、约150、约155、或更多个氨基酸置换、缺失或插入。用于此类操作的方法在本领域内通常是己知的。例如，通过在DNA中的突变可以制备杀有害生物蛋白的氨基酸序列变体。这还可以通过几种诱变形式之一和/或在定向进化中来完成。在某些方面，在该氨基酸序列中所编码的改变将基本上不影响该蛋白的功能。这样的变体将具有所希望的杀有害生物活性。然而，应当理解，可以通过使用这样的技术改进杀有害生物蛋白对本发明的这些组合物所赋予的杀有害生物活性的能力。例如，可以在以下宿主细胞中表达杀有害生物蛋白，这些宿主细胞在DNA复制过程中显示出高比率的碱基错掺，如XL-1Red(Stratagene公司，拉荷亚(La Jolla)，加利福尼亚州)。在这样的菌株中繁殖之后，可以分离出DNA(例如通过制备质粒DNA，或通过由PCR进行扩增并且将得到的PCR片段克隆到载体中)，在非诱变菌株中培养这些杀有害生物蛋白突变体，并且鉴定具有杀有害生物活性的经突变的基因，例如通过进行一项对杀有害生物活性进行测试的测定。通常，在摄食测定中混合并使用了这种蛋白。参见，例如马罗内(Marrone)等人(1985)经济昆虫学杂志(J.of Economic Entomology)78：290-293。这样的测定可以包括使植物与一种或多种有害生物接触并且确定该植物存活和/或造成这些有害生物死亡的能力。导致毒性增加的突变的实例见于史涅夫(Schnepf)等人(1998)微生物学与分子生物学评论(Microbiol.Mol.Biol.Rev.)62：775-806。
可替代地，可以在氨基或羧基的末端处对多种蛋白的蛋白序列进行改变，而基本上不影响活性。这可以包括通过现代分子方法所引入的插入、缺失、或改变，这些方法如PCR，包括PCR扩增，这些PCR扩增借助于将编码氨基酸的序列包括到在PCR扩增中所使用的寡核苷酸之中而改变或延长了该编码蛋白的序列。可替代地，所加入的蛋白序列可以包括完整的编码蛋白的序列，如在本领域内通常用于产生蛋白融合物的那些序列。这样的融合蛋白常常用于(1)增加感兴趣的蛋白的表达；(2)引入结合结构域、酶活性、或表位以促进蛋白纯化、蛋白检测、或本领域已知的其他实验用途；或(3)将蛋白的分泌或翻译靶向亚细胞器，如革兰氏阴性菌的壁膜间隙，或真核细胞的内质网，后者常常导致蛋白的糖基化。
本发明的变体核苷酸和氨基酸序列还涵盖了由诱变和致重组操作程序(如DNA改组)所衍生的序列。使用这样的操作，可以将一个或多个不同的杀有害生物蛋白编码区用于创造出具有所希望的性质的新杀有害生物蛋白。这样，由一群相关的序列多核苷酸产生重组多核苷酸文库，这些多核苷酸包含具有基本序列一致性并且能够在体外或体内同源重组的序列区。例如，使用这种方法，可以将编码感兴趣的结构域的序列基序在本发明的杀有害生物蛋白基因与其他已知的杀有害生物蛋白基因之间进行改组，以获得编码蛋白的新基因，该蛋白具有改进的感兴趣的性质，例如增加的杀昆虫活性。用于这样的DNA改组的策略在本领域内是己知的。参见例如施特默尔(Stemmer)(1994)美国国家科学院院刊91：10747-10751；施特默尔(1994)自然(Nature)370：389-391；卡莫瑞(Crameri)等人(1997)自然生物技术(Nature Biotech.)15：436-438；摩尔(Moore)等人(1997)分子生物学杂志272：336-347；张(Zhang)等人(1997)美国国家科学院院刊94：4504-4509；卡莫瑞等人(1998)自然391：288-291；以及美国专利号5,605,793和5,837,458。
结构域交换或改组是针对产生改变的杀有害生物蛋白的另一种机制。可在杀有害生物蛋白之间交换结构域，从而导致具有改进的杀有害生物活性或靶标谱的杂合或嵌合毒素。用于产生重组蛋白和测试它们的杀有害生物活性的方法在本领域是熟知的(参见，例如，纳莫夫(Naimov)等人(2001)应用与环境微生物学(Appl.Environ.Microbiol.)67：5328-5330；戴马格德(de Maagd)等人(1996)应用与环境微生物学62：1537-1543；格(Ge)等人(1991)生物化学杂志(J.Biol.Chem.)266：17954-17958；史涅夫等人(1990)生物化学杂志265：20923-20930；兰格(Rang)等人(1999)应用与环境微生物学65：2918-2925)。
在又另一个实施例中，使用以下中的一项或多项可获得变体核苷酸和/或氨基酸序列：易错PCR、寡核苷酸定向诱变、组装PCR、有性PCR诱变、体内诱变、盒式诱变、递归整体诱变、指数整体诱变、位点特异性诱变、基因重组装、基因位点饱和诱变、置换诱变、合成连接重组装(SLR)、重组、递归序列重组、磷酸酯修饰的DNA诱变、含有尿嘧啶的模板诱变、缺口双链体诱变、点错配修复诱变、修复缺陷宿主菌株诱变、化学诱变、辐射所致诱变、缺失诱变、限制选择诱变、限制纯化诱变、人工基因合成、整体诱变、嵌合核酸多聚体建立等。
载体
本发明的杀有害生物序列可以被提供在用于在感兴趣的植物中表达的表达盒中。“植物表达盒”是指DNA构建体，该构建体能够导致蛋白在植物细胞中从开放阅读框中的表达。典型地，这些构建体包括启动子和编码序列。经常，此类构建体还将包含3'非翻译区。此类构建体可以包含“信号序列”或“前导序列”，以促进该肽的共翻译成或翻译后运输至某些细胞内结构，如叶绿体(或其他质体)、内质网、或高尔基体。
“信号序列”是指如下序列，已知或怀疑该序列导致穿过细胞膜的共翻译或翻译后肽运输。在真核生物中，这典型地涉及分泌到高尔基体内，其中具有某些产生的糖基化。细菌的杀昆虫毒素经常被合成为原毒素，它们是在靶标有害生物的肠中被蛋白水解激活(常(Chang)(1987)酶学方法(Methods Enzymol.)153：507-516)。在本发明的一些实施例中，信号序列位于天然序列中，或可以衍生自本发明的序列。“前导序列”是指当被翻译时导致足以触发肽链的共翻译运输至亚细胞器的氨基酸序列的任何序列。因此，这包括通过进入内质网内、进入液泡、质体(包括叶绿体、线粒体)等中来对运输和/或糖基化进行靶向的前导序列。
“植物转化载体”是指DNA分子，该分子对于有效转化植物细胞而言是所必需的。这种分子可以由一个或多个植物表达盒组成，或可以被组织进入超过一个的“载体”DNA分子中。例如，二元载体是植物转化载体，它们利用两个非连续DNA载体来编码用于植物细胞转化的所有需求的顺式和反式作用功能(海伦斯(Hellens)和姆林内克斯(Mullineaux)(2000)植物科学趋势(Trends in Plant Science)5：446-451)。“载体”是指用于在不同的宿主细胞之间进行转移的核酸构建体。“表达载体”是指载体，该载体具有将异源的DNA序列或片段结合、整合到外源细胞中并且在其中表达该异源DNA序列或片段的能力。该盒将包括与本发明的序列可操作地连接的5'和/或3'调节序列。“可操作地连接”是指在启动子与第二序列之间的功能性连接，其中该启动子序列引发并介导了对应于该第二序列的DNA序列的转录。一般来说，可操作地连接是指所连接的核酸序列是连续的，并且当需要接合两个蛋白编码区时，是连续的并且处于同一读码框中。在一些实施例中，核苷酸序列被可操作地连接至能够指导所述核苷酸序列在宿主细胞(如微生物宿主细胞或植物宿主细胞)中的表达的异源启动子。该盒可以另外地包括至少一种有待共转化到生物中的另外的基因。可替代地，可以在多个表达盒上提供这种或这些另外的基因。
在不同实施例中，本发明的核苷酸序列被可操作地连接至启动子，例如植物启动子。“启动子”是指核酸序列，该核酸序列发挥作用以指导下游编码序列的转录。该启动子连同其他转录和翻译的调节性核酸序列(也被称为“控制序列”)是感兴趣的DNA序列的表达所必需的。
这种表达盒配备有多个限制位点，这些限制位点用于插入该杀有害生物序列以便处于这些调节区的转录调节之下。
该表达盒将以5'-3'转录方向包括转录和翻译起始区(即，启动子)、本发明的DNA序列以及在植物中发挥作用的翻译和转录终止区(即，终止区)。该启动子对于该植物宿主和/或本发明的DNA序列而言可以是天然的或类似的、或外源的或异源的。另外地，该启动子可以是天然序列或可替代地是合成序列。在该启动子对于该植物宿主而言是“天然的”或“同源的”时，它是指该启动子是在该启动子所引入的天然植物中被发现的。在启动子对于本发明的DNA序列而言是“外源的”或“异源的”时，它是指该启动子对于可操作地连接的本发的明DNA序列而言不是天然的或自然发生的启动子。
该终止区对于转录起始区而言可以是天然的，对于可操作地连接的感兴趣的DNA序列而言可以是天然的，对于该植物宿主而言可以是天然的，或可以衍生自另一种来源(即，对于该启动子、该感兴趣的DNA序列、该植物宿主、或它们的任何组合而言是外源的或异源的)。方便的终止区可以获自根瘤土壤杆菌(A.tumefaciens)的Ti质粒，如章鱼碱合成酶和胭脂碱合成酶的终止区。还参见，乔瑞诺(Guerineau)等人(1991)分子遗传学与普通遗传学(Mol.Gen.Genet.)262：141-144；普劳德富特(Proudfoot)(1991)细胞(Cell)64：671-674；桑斯法冈(Sanfacon)等人(1991)基因与发育(Genes Dev.)5：141-149；摩根(Mogen)等人(1990)植物细胞(Plant Cell)2：1261-1272；芒罗(Munroe)等人(1990)基因(Gene)91：151-158；巴拉斯(Ballas)等人(1989)核酸研究(Nucleic Acids Res.)17：7891-7903；以及乔希(Joshi)等人(1987)核酸研究15：9627-9639)。
在适当情况下，可以针对在转化的宿主细胞中的增加的表达来优化这种或这些基因。即，可以使用宿主细胞偏爱的密码子来合成这些基因以用于改进的表达，或者能以宿主偏爱的密码子使用频率使用密码子来合成这些基因。通常，该基因的GC含量会增加。参见，例如，坎贝尔(Campbell)和哥瑞(Gowri)(1990)植物生理学(Plant Physiol.)92：1-11，它讨论了宿主偏爱的密码子使用。在本领域中用于合成植物偏好基因的多种方法是可获得的。参见例如美国专利号5,380,831和5,436,391、美国专利公开号20090137409、以及莫里(Murray)等人(1989)核酸研究17：477-498，它们通过引用结合在此。
在一个实施例中，将杀有害生物蛋白靶向叶绿体而用于表达。按照这种方式，在该杀有害生物蛋白未直接被插到该叶绿体中时，该表达盒将另外地包括编码转运肽的核酸以便将该杀有害生物蛋白导向该叶绿体。这些转运肽在本领域是已知的。参见例如，冯·海耶(Von Heijne)等人(1991)植物分子生物学导报(Plant Mol.Biol.Rep.)9：104-126；克里克(Clark)等人(1989)生物化学杂志264：17544-17550；岱拉-乔帕(Della-Cioppa)等人(1987)植物生理学84：965-968；罗默(Romer)等人(1993)生物化学和生物物理研究通讯(Biochem.Biophys.Res.Commun.)196：1414-1421；以及沙阿(Shah)等人(1986)科学(Science)233：478-481。
可以针对在叶绿体中的表达来优化被靶向该叶绿体的杀有害生物基因，以便考虑在该植物细胞核与这种细胞器之间在密码子使用方面的差异。按照这种方式，可以使用叶绿体偏爱的密码子来合成感兴趣的核酸。参见，例如，美国专利号5,380,831，该专利通过引用结合在此。
植物转化
本发明的方法涉及将核苷酸构建体引入到植物中。“引入”是指按照以下方式将该核苷酸构建体给予该植物，使得该构建体获得了接近该植物的细胞的内部的途径。本发明的方法不需要使用将核苷酸构建体引入植物中的具体方法，只需要该核苷酸构建体获得接近该植物的至少一个细胞的内部的途径。用于将核苷酸构建体引入到植物中的方法是本领域已知的，这些方法包括但不限于：稳定转化法、瞬时转化法、以及病毒介导法。
“植物”是指整个植物、植物器官(例如，叶子、茎、根、等)、种子、植物细胞、繁殖体、胚芽以及以上的子代。植物细胞可以是分化的或未分化的(例如，愈伤组织、悬浮培养细胞、原生质体、叶细胞、根细胞、韧皮部细胞、花粉)。
“转基因植物”或“经转化的植物”或“稳定转化的”植物或细胞或组织是指已经将外源的核酸序列或DNA片段结合或整合到该植物细胞中的植物。这些核酸序列包括外源的、或不存在于该未转化的植物细胞中的那些序列、以及可以是内源的、或存在于该未转化的植物细胞中的那些序列。“异源的”大体上是指以下核酸序列，这些核酸序列对于它们所在的细胞或天然基因组的一部分而言不是内源的，并且已经通过感染、转染、显微注射、电穿孔、微粒轰击、或类似方法加到该细胞中。
本发明的转基因植物表达了在此披露的新颖的毒素序列中的一种或多种。在一些实施例中，本发明的蛋白或核苷酸序列在植物中与编码向此类植物赋予有用的农艺特性的蛋白或RNA的其他基因有利地组合。在编码为转化的植物赋予有用的农艺特性的蛋白或RNA的基因之中，可以提及编码赋予对一种或多种除草剂的耐受性的蛋白、以及赋予对某些昆虫的耐受性的其他蛋白、赋予对某些疾病的耐受性的那些蛋白的DNA序列，编码提供线虫或昆虫控制等的RNA的DNA。此类基因特别描述在公开的PCT专利申请WO 91/02071和WO 95/06128以及美国专利7,923,602和美国专利申请公开号20100166723，将它们各自通过引用以其全文结合在此。
在编码为转化的植物细胞和植物赋予对某些除草剂的耐受性的蛋白的DNA序列中，可以提及赋予对草铵膦除草剂的耐受性的bar或PAT基因或天蓝色链霉菌基因(描述于WO 2009/152359中)、编码赋予对以EPSPS作为靶标的除草剂的耐受性的合适的EPSPS(如草甘膦及其盐)的基因(US 4,535,060、US 4,769,061、US 5,094,945、US 4,940,835、US 5,188,642、US 4,971,908、US 5,145,783、US 5,310,667、US 5,312,910、US 5,627,061、US5,633,435)、编码草甘膦-正-乙酰转移酶的基因(例如US 8,222,489、US 8,088,972、US 8,044,261、US 8,021,857、US 8,008,547、US 7,999,152、US 7,998,703、US 7,863,503、US7,714,188、US 7,709,702、US 7,666,644、US 7,666,643、US 7,531,339、US 7,527,955、以及US 7,405,074)、编码草甘膦氧化还原酶的基因(例如US 5,463,175)、或编码HPPD抑制剂耐受蛋白的基因(例如，该HPPD抑制剂耐受基因描述于WO 2004/055191、WO 199638567、US6791014、WO 2011/068567、WO 2011/076345、WO 2011/085221、WO 2011/094205、WO 2011/068567、WO 2011/094199、WO 2011/094205、WO 2011/145015、WO 2012056401、以及PCT/US2013/59598中)。
在编码合适的EPSPS(其赋予对以EPSPS为靶标的除草剂的耐受性)的DNA序列中，更特别地提及如下基因，该基因编码植物EPSPS，特别是玉米EPSPS，特别是包括两个突变(特别是氨基酸位置102处突变和氨基酸位置106处突变)的玉米EPSPS的基因(WO 2004/074443)，并且描述于专利申请US 6566587，在下文命名为双突变型玉米EPSPS或2mEPSPS，或如下基因，该基因编码从土壤杆菌属分离的EPSPS并且通过US专利5,633,435的序列ID号2和序列ID号3描述，也称为CP4。
在编码合适的EPSPS(其赋予对以EPSPS为靶标的除草剂的耐受性)的DNA序列中，更特别地提及如下基因，该基因编码来自球形节杆菌的EPSPS GRG23，以及还有突变体GRG23ACE1、GRG23ACE2、或GRG23ACE3，特别是描述于WO 2008/100353中的GRG23的突变体或变体，例如WO 2008/100353中的SEQ ID No.29的GRG23(ace3)R173K。
在编码EPSPS的DNA序列，并且更特别地编码上述基因的情况下，编码这些酶的序列有利地在编码过渡肽(特别是描述于US专利5,510,471或5,633,448中的“优化转运肽”)的序列前面。
可以与本发明的核酸序列组合的示例性的除草剂耐受性性状进一步包括至少一种ALS(乙酰乳酸合酶)抑制剂(WO 2007/024782)；突变的拟南芥属ALS/AHAS基因(美国专利6,855,533)；编码通过新陈代谢赋予对2,4-D(2,4-二氯苯氧乙酸)的耐受性的2,4-D-单加氧酶的基因(美国专利6,153,401)；以及编码通过新陈代谢赋予对麦草畏(3,6-二氯-2-甲氧基苯甲酸)的耐受性的麦草畏单加氧酶的基因(US 2008/0119361和US 2008/0120739)。
在不同的实施例中，本发明的核酸与一个或多个除草剂耐受基因叠加，该一个或多个除草剂耐受基因包括一个或多个HPPD抑制剂除草剂耐受基因、和/或耐草甘膦和/或草铵膦的一个或多个基因。
在编码涉及对昆虫的耐受性的特性的蛋白的DNA序列中，更特别地提及在文献中广泛描述并且本领域的普通技术人员熟知的Bt蛋白。也将提及从细菌如发光杆菌属提取的蛋白(WO 97/17432&WO 98/08932)。
在编码赋予对昆虫的耐受性的新颖特性的感兴趣的蛋白的此类DNA序列中，更特别地提及在文献中广泛描述并且本领域的普通技术人员熟知的Bt Cry或VIP蛋白。这些包括衍生自Cry1F蛋白的Cry1F蛋白或杂交体(例如，US 6,326,169；US 6,281,016；US 6,218,188中所描述的杂合Cry1A-Cry1F蛋白或其毒性片段)，Cry1A-型蛋白或其毒性片段(优选衍生自Cry1Ac蛋白的Cry1Ac蛋白或杂交体)(例如，US 5,880,275中所描述的杂合Cry1Ab-Cry1Ac蛋白)或如EP 451878中所描述的Cry1Ab或Bt2蛋白或其杀昆虫片段，如WO 2002/057664中所描述的Cry2Ae、Cry2Af或Cry2Ag蛋白或其毒性片段，WO 2007/140256中所描述的Cry1A.105蛋白(SEQ ID No.7)或其毒性片段，NCBI登录号ABG20428的VIP3Aa19蛋白，NCBI登录号ABG20429的VIP3Aa20蛋白(SEQ ID No.2，于WO 2007/142840中)，COT202或COT203棉花事件中产生的VIP3A蛋白(分别地为WO 2005/054479和WO 2005/054480)，如WO2001/47952中所描述的Cry蛋白，VIP3Aa蛋白或其毒性片段(如描述于埃斯特鲁奇(Estruch)等人(1996)，美国科学院院刊28；93(11)：5389-94和US 6,291,156)，来自致病杆菌属(如WO 98/50427中描述的)、沙雷氏菌属(特别上来自嗜虫沙雷氏菌)或发光杆菌属物种菌株的杀昆虫蛋白，例如来自发光杆菌属的Tc-蛋白(如描述于WO 98/08932中)(例如沃特菲尔德(Waterfield)等人，2001，应用与环境微生物学(Appl Environ Microbiol.)67(11)：5017-24；法伦许-康斯坦特(Ffrench-Constant)和鲍恩(Bowen)，2000，细胞与分子生命科学(Cell Mol Life Sci.)；57(5)：828-33)。在此还包括了与上述序列中任一个在一些个(1-10个，优选1-5个)氨基酸上不同的这些蛋白中的任一个的任何变体或突变体，特别是它们的毒性片段的序列，或它们被稠合至转运肽，如质体转运肽、或另一种蛋白或肽。
在不同的实施例中，本发明的核酸可以在植物中与赋予所希望性状的一个或多个基因组合，这些性状如除草剂耐受性、昆虫耐受性、耐旱性、线虫控制、水分利用效率、氮利用效率、改进的营养价值、抗病性、改进的光合作用、改进的纤维质量、胁迫耐受性、改进的繁殖等。
在相同物种的植物中可与本发明的基因组合的特别有用的转基因事件(例如，通过杂交或通过重新转化植物，该植物含有以本发明的嵌合基因进行的另一个转基因事件)包括事件531/PV-GHBK04(棉花，昆虫控制，描述于WO 2002/040677中)，事件1143-14A(棉花，昆虫控制，未保藏，描述于WO 2006/128569中)；事件1143-51B(棉花，昆虫控制，未保藏，描述于WO 2006/128570中)；事件1445(棉花，除草剂耐受，未保藏，描述于US-A 2002-120964或WO 2002/034946事件17053(水稻，除草剂耐受，保藏号为PTA-9843，描述于WO2010/117737中)；事件17314(水稻，除草剂耐受，保藏号为PTA-9844，描述于WO 2010/117735中)；事件281-24-236(棉花，昆虫控制-除草剂耐受，保藏号为PTA-6233，描述于WO2005/103266或US-A2005-216969中)；事件3006-210-23(棉花，昆虫控制-除草剂耐受，保藏号为PTA-6233，描述于US-A 2007-143876或WO 2005/103266中)；事件3272(玉米，质量性状，保藏号为PTA-9972，描述于WO 2006/098952或US-A 2006-230473中)；事件33391(小麦，除草剂耐受，保藏号为PTA-2347，描述于WO 2002/027004中)，事件40416(玉米，昆虫控制-除草剂耐受，保藏号为ATCC PTA-11508，描述于WO 11/075593中)；事件43A47(玉米，昆虫控制-除草剂耐受，保藏号为ATCC PTA-11509，描述于WO2011/075595中)；事件5307(玉米，昆虫控制，保藏号为ATCC PTA-9561，描述于WO 2010/077816中)；事件ASR-368(翦股颖，除草剂耐受，保藏号为ATCC PTA-4816，描述于US-A 2006-162007或WO 2004/053062中)；事件B16(玉米，除草剂耐受，未保藏，描述于US-A 2003-126634中)；事件BPS-CV127-9(大豆，除草剂耐受，保藏号为NCIMB No.41603，描述于WO 2010/080829中)；事件BLR1(油菜，恢复雄性不育，保藏号为NCIMB 41193，描述于WO 2005/074671中)，事件CE43-67B(棉花，昆虫控制，保藏号为DSM ACC2724，描述于US-A 2009-217423或WO 2006/128573中)；事件CE44-69D(棉花，昆虫控制，未保藏，描述于US-A 2010-0024077中)；事件CE44-69D(棉花，昆虫控制，未保藏，描述于WO 2006/128571中)；事件CE46-02A(棉花，昆虫控制，未保藏，描述于WO2006/128572中)；事件COT102(棉花，昆虫控制，未保藏，描述于US-A 2006-130175或WO2004/039986中)；事件COT202(棉花，昆虫控制，未保藏，描述于US-A 2007-067868或WO2005/054479中)；事件COT203(棉花，昆虫控制，未保藏，描述于WO 2005/054480中)；事件DAS21606-3/1606(大豆，除草剂耐受，保藏号为PTA-11028，描述于WO 2012/033794中)，事件DAS40278(玉米，除草剂耐受，保藏号为ATCC PTA-10244，描述于WO 2011/022469中)；事件DAS-44406-6/pDAB8264.44.06.1(大豆，除草剂耐受，保藏号为PTA-11336，描述于WO2012/075426中)，事件DAS-14536-7/pDAB8291.45.36.2(大豆，除草剂耐受，保藏号为PTA-11335，描述于WO 2012/075429中)，事件DAS-59122-7(玉米，昆虫控制-除草剂耐受，保藏号为ATCC PTA 11384，描述于US-A 2006-070139中)；事件DAS-59132(玉米，昆虫控制-除草剂耐受，未保藏，描述于WO 2009/100188中)；事件DAS68416(大豆，除草剂耐受，保藏号为ATCCPTA-10442，描述于WO 2011/066384或WO 2011/066360中)；事件DP-098140-6(玉米，除草剂耐受，保藏号为ATCC PTA-8296，描述于US-A 2009-137395或WO 08/112019中)；事件DP-305423-1(大豆，质量性状，未保藏，描述于US-A 2008-312082或WO 2008/054747中)；事件DP-32138-1(玉米，杂交系统，保藏号为ATCC PTA-9158，描述于US-A 2009-0210970或WO2009/103049中)；事件DP-356043-5(大豆，除草剂耐受，保藏号为ATCC PTA-8287，描述于US-A 2010-0184079或WO 2008/002872中)；事件EE-1(茄子，昆虫控制，未保藏，描述于WO07/091277中)；事件FI117(玉米，除草剂耐受，保藏号为ATCC 209031，描述于US-A 2006-059581或WO 98/044140中)；事件FG72(大豆，除草剂耐受，保藏号为PTA-11041，描述于WO2011/063413中)，事件GA21(玉米，除草剂耐受，保藏号为ATCC 209033，描述于US-A 2005-086719或WO 98/044140中)；事件GG25(玉米，除草剂耐受，保藏号为ATCC 209032，描述于US-A 2005-188434或WO 98/044140中)；事件GHB119(棉花，昆虫控制-除草剂耐受，保藏号为ATCC PTA-8398，描述于WO 2008/151780中)；事件GHB614(棉花，除草剂耐受，保藏号为ATCC PTA-6878，描述于US-A 2010-050282或WO 2007/017186中)；事件GJ11(玉米，除草剂耐受，保藏号为ATCC 209030，描述于US-A 2005-188434或WO 98/044140中)；事件GM RZ13(甜菜，抗病毒，保藏号为NCIMB-41601，描述于WO 2010/076212中)；事件H7-1(甜菜，除草剂耐受，保藏号为NCIMB41158或NCIMB 41159，描述于US-A 2004-172669或WO 2004/074492中)；事件JOPLIN1(小麦，疾病耐受，未保藏，描述于US-A 2008-064032中)；事件LL27(大豆，除草剂耐受，保藏号为NCIMB41658，描述于WO 2006/108674或US-A 2008-320616中)；事件LL55(大豆，除草剂耐受，保藏号为NCIMB41660，描述于WO 2006/108675或US-A 2008-196127中)；事件LL棉花25(棉花，除草剂耐受，保藏号为ATCC PTA-3343，描述于WO 2003/013224或US-A2003-097687中)；事件LLRICE06(水稻，除草剂耐受，保藏号为ATCC 203353，描述于US 6,468,747或WO 2000/026345中)；事件LLRice62(水稻，除草剂耐受，保藏号为ATCC 203352，描述于WO 2000/026345)，事件LLRICE601(水稻，除草剂耐受，保藏号为ATCCPTA-2600，描述于US-A 2008-2289060或WO 2000/026356中)；事件LY038(玉米，质量性状，保藏号为ATCC PTA-5623，描述于US-A 2007-028322或WO 2005/061720中)；事件MIR162(玉米，昆虫控制，保藏号为PTA-8166，描述于US-A 2009-300784或WO 2007/142840中)；事件MIR604(玉米，昆虫控制，未保藏，描述于US-A2008-167456或WO 2005/103301中)；事件MON15985(棉花，昆虫控制，保藏号为ATCC PTA-2516，描述于US-A 2004-250317或WO 2002/100163中)；事件MON810(玉米，昆虫控制，未保藏，描述于US-A 2002-102582中)；事件MON863(玉米，昆虫控制，保藏号为ATCC PTA-2605，描述于WO 2004/011601或US-A 2006-095986中)；事件MON87427(玉米，传粉控制，保藏号为ATCC PTA-7899，描述于WO 2011/062904中)；事件MON87460(玉米，胁迫耐受，保藏号为ATCC PTA-8910，描述于WO 2009/111263或US-A2011-0138504中)；事件MON87701(大豆，昆虫控制，保藏号为ATCC PTA-8194，描述于US-A 2009-130071或WO 2009/064652中)；事件MON87705(大豆，质量性状-除草剂耐受，保藏号为ATCC PTA-9241，描述于US-A2010-0080887或WO 2010/037016中)；事件MON87708(大豆，除草剂耐受，保藏号为ATCC PTA-9670，描述于WO 2011/034704中)；事件MON87712(大豆，产量，保藏号为PTA-10296，描述于WO 2012/051199中)，事件MON87754(大豆，质量性状，保藏号为ATCC PTA-9385，描述于WO 2010/024976中)；事件MON87769(大豆，质量性状，保藏号为ATCC PTA-8911，描述于US-A2011-0067141或WO 2009/102873中)；事件MON88017(玉米，昆虫控制-除草剂耐受，保藏号为ATCC PTA-5582，描述于US-A 2008-028482或WO 2005/059103中)；事件MON88913(棉花，除草剂耐受，保藏号为ATCC PTA-4854，描述于WO 2004/072235或US-A 2006-059590中)；事件MON88302(油菜，除草剂耐受，保藏号为PTA-10955，描述于WO 2011/153186中)，事件MON88701(棉花，除草剂耐受，保藏号为PTA-11754，描述于WO 2012/134808中)，事件MON89034(玉米，昆虫控制，保藏号为ATCC PTA-7455，描述于WO 07/140256或US-A 2008-260932中)；事件MON89788(大豆，除草剂耐受，保藏号为ATCC PTA-6708，描述于US-A 2006-282915或WO 2006/130436中)；事件MS11(油菜，传粉控制-除草剂耐受，保藏号为ATCC PTA-850或PTA-2485，描述于WO 2001/031042中)；事件MS8(油菜，传粉控制-除草剂耐受，保藏号为ATCC PTA-730，描述于WO 2001/041558或US-A 2003-188347中)；事件NK603(玉米，除草剂耐受，保藏号为ATCC PTA-2478，描述于US-A2007-292854中)；事件PE-7(水稻，昆虫控制，未保藏，描述于WO 2008/114282中)；事件RF3(油菜，传粉控制-除草剂耐受，保藏号为ATCC PTA-730，描述于WO 2001/041558或US-A2003-188347中)；事件RT73(油菜，除草剂耐受，未保藏，描述于WO 2002/036831或US-A2008-070260中)；事件SYHT0H2/SYN-000H2-5(大豆，除草剂耐受，保藏号为PTA-11226，描述于WO 2012/082548中)，事件T227-1(甜菜，除草剂耐受，未保藏，描述于WO 2002/44407或US-A 2009-265817中)；事件T25(玉米，除草剂耐受，未保藏，描述于US-A 2001-029014或WO2001/051654中)；事件T304-40(棉花，昆虫控制-除草剂耐受，保藏号为ATCC PTA-8171，描述于US-A 2010-077501或WO 2008/122406中)；事件T342-142(棉花，昆虫控制，未保藏，描述于WO 2006/128568中)；事件TC1507(玉米，昆虫控制-除草剂耐受，未保藏，描述于US-A2005-039226或WO 2004/099447中)；事件VIP 1034(玉米，昆虫控制-除草剂耐受，保藏号为ATCC PTA-3925.，描述于WO 2003/052073中)，事件32316(玉米，昆虫控制-除草剂耐受，保藏号为PTA-11507，描述于WO 2011/084632中)，事件4114(玉米，昆虫控制-除草剂耐受，保藏号为PTA-11506，描述于WO 2011/084621中)，事件EE-GM3/FG72(大豆，除草剂耐受，ATCC登录号PTA-11041)，任选地与事件EE-GM1/LL27或事件EE-GM2/LL55(WO 2011/063413A2)叠加，事件DAS-68416-4(大豆，除草剂耐受，ATCC登录号PTA-10442，WO 2011/066360A1)，事件DAS-68416-4(大豆，除草剂耐受，ATCC登录号PTA-10442，WO 2011/066384A1)，事件DP-040416-8(玉米，昆虫控制，ATCC登录号PTA-11508，WO 2011/075593A1)，事件DP-043A47-3(玉米，昆虫控制，ATCC登录号PTA-11509，WO 2011/075595A1)，事件DP-004114-3(玉米，昆虫控制，ATCC登录号PTA-11506，WO 2011/084621Al)，事件DP-032316-8(玉米，昆虫控制，ATCC登录号PTA-11507，WO 2011/084632A1)，事件MON-88302-9(油菜，除草剂耐受，ATCC登录号PTA-10955，WO 2011/153186 A1)，事件DAS-21606-3(大豆，除草剂耐受，ATCC登录号PTA-11028，WO 2012/033794A2)，事件MON-87712-4(大豆，质量性状，ATCC登录号PTA-10296，WO 2012/051199A2)，事件DAS-44406-6(大豆，叠加除草剂耐受，ATCC登录号PTA-11336，WO2012/075426A1)，事件DAS-14536-7(大豆，叠加除草剂耐受，ATCC登录号PTA-11335，WO 2012/075429A1)，事件SYN-000H2-5(大豆，除草剂耐受，ATCC登录号PTA-11226，WO 2012/082548A2)，事件DP-061061-7(油菜，除草剂耐受，无可获得的保藏号，WO2012071039A1)，事件DP-073496-4(油菜，除草剂耐受，无可获得的保藏号，US2012131692)，事件8264.44.06.1(大豆，叠加除草剂耐受，登录号PTA-11336，WO2012075426A2)，事件8291.45.36.2(大豆，叠加除草剂耐受，登录号PTA-11335，WO2012075429A2)，事件SYHT0H2(大豆，ATCC登录号PTA-11226，WO 2012/082548A2)，事件MON88701(棉花，ATCC登录号PTA-11754，WO 2012/134808A1)，事件KK179-2(苜蓿，ATCC登录号PTA-11833，WO 2013/003558A1)，事件pDAB8264.42.32.1(大豆，叠加除草剂耐受，ATCC登录号PTA-11993，WO 2013/010094A1)，事件MZDT09Y(玉米，ATCC登录号PTA-13025，WO 2013/012775A1)。
通过在本领域内已知的几种技术之一可以实现植物细胞的转化。可以对本发明的杀有害生物基因进行修饰以便在植物细胞中获得或增强表达。典型地，表达这种蛋白的构建体将包含驱动该基因的转录的启动子，连同允许转录终止和多腺苷酸化的3'非翻译区。这样的构建体的组织在本领域内是熟知的。在一些情况下，可能有用的是将该基因工程化使得所得到的肽被分泌、或者以其他方式靶向在植物细胞中。例如，该基因可以被工程化以包括信号肽来促进该肽转移到内质网。还可以优选使该植物表达盒工程化以包括内含子，使得该内含子的mRNA加工是表达所需要的。
典型地，这种“植物类表达盒”将被插入“植物转化载体”中。这种植物转化载体可以包括对于实现植物转化所需要的一个或多个DNA载体。例如，利用包括超过一个连续DNA区段的植物转化载体在本领域内是常见的实践。在本领域内，这些载体经常被称为“二元载体”。二元载体以及具有辅助质粒的载体被最通常地用于土壤杆菌介导的转化，其中对于实现有效转化所需要的DNA区段的大小以及复杂度是相当大的，并且将多种功能分到分离的DNA分子上是有利的。二元载体典型地包含质粒载体，该质粒载体包含对于T-DNA转移所要求的顺式作用序列(如左边界和右边界)、经工程化处理以便能够在植物细胞中表达的选择标记物、以及“感兴趣的基因”(经工程化处理以便能够在植物细胞中表达的基因，对于它而言转基因植物的产生是所希望的)。在这种质粒载体上还存在着细菌复制所需要的序列。这些顺式作用序列以如下方式进行排列以便允许有效转移到植物细胞中并在其中进行表达。例如，该选择标记基因以及该杀有害生物基因位于该左边界与右边界之间。通常第二质粒载体包含反式作用因子，这些反式作用因子介导T-DNA从土壤杆菌属到植物细胞的转化。这种质粒经常包含这些毒性功能(Vir基因)，这些毒性功能允许由土壤杆菌感染植物细胞，并且通过在边界序列上的切割以及vir-介导的DNA转移来转移DNA，如在本领域中所理解(海伦斯(Hellens)和马利诺(Mullineaux)(2000)植物科学趋势(Trends in Plant Science)5：446-451)。几种类型的土壤杆菌属菌株(例如，LBA4404、GV3101、EHA101、EHA105、等)可以用于植物转化。该第二质粒载体是通过其他方法(如微粒轰击、显微注射、电穿孔、聚乙二醇、等)转化这些植物所不需要的。
通常，植物转化法类涉及将异源DNA转移到靶标植物细胞(例如，未成熟或成熟的胚、悬浮培养物、未分化的愈伤组织、原生质体，等等)中，之后跟随应用最大阈值水平的合适选择(取决于这种选择标记基因)，以便从一群未转化的细胞团中回收这些转化的植物细胞。典型地将外植体转移到新鲜供应的相同培养基中并将其常规培养。随后，在被置于补充有最大阈值水平的选择试剂的再生培养基上之后，这些转化的细胞分化成芽。然后，将这些芽转移到选择性生根培养基中用于回收已生根的芽或小植株。随后，转基因小植株生长成成熟植物并产生能育种子(例如，日江井(Hiei)等人(1994)植物杂志(The Plant Journal)6：271-282；石田(Ishida)等人(1996)自然生物技术(Nature Biotechnology)14：745-750)。典型地将外植体转移到新鲜供应的相同培养基中并将其常规培养。用于产生转基因植物的技术和方法的一般说明可见于艾尔丝(Ayres)和帕克(Park)(1994)植物科学的关键性评论(Critical Reviews in Plant Science)13：219-239以及柏木妮(Bommineni)和裘哈尔(Jauhar)(1997)Maydica 42：107-120。因为这种转化的材料包括多个细胞；转化的和未转化的细胞二者都存在于受试的靶标愈伤组织或细胞组织或组群的任何部分中。杀死未转化的细胞并允许转化的细胞进行增殖的能力导致了转化的植物培养物。经常，去除未转化的细胞的能力是快速回收转化的植物细胞以及成功产生转基因植物的限制。
转化方案以及用于将核苷酸序列引入到植物中的方案可依据靶向用于转化的植物或植物细胞的类型(即，单子叶或双子叶)而变化。转基因植物的产生可以通过几种方法之一来进行，包括但不限于：显微注射、电穿孔、直接基因转移、通过土壤杆菌将异源DNA引入植物细胞中(土壤杆菌-介导的转化)、用粘附于粒子上的异源外源DNA来轰击植物细胞、射弹粒子加速(ballistic particle acceleration)、气溶胶束转化(aerosol beamtransformation)(美国公开申请号20010026941；美国专利号4,945,050；国际公开号WO91/00915；美国公开申请号2002015066)、Lec1转化、以及用于转移DNA的不同的其他非粒子直接介导法。
用于叶绿体转化的方法在本领域内是己知的。参见，例如，什瓦布(Svab)等人(1990)美国国家科学院院刊87：8526-8530；什瓦布和马利加(Maliga)(1993)美国国家科学院院刊90：913-917；什瓦布和马利加(1993)欧洲分子生物学杂志(EMBO J.)12：601-606。该方法依靠将含有选择标记的DNA的粒子枪递送到质体基因组中并且通过同源重组将该DNA靶向该质体基因组中。另外，质体转化可以通过核编码的以及质体指导的RNA聚合酶的组织偏爱的表达、通过沉默的携带质体的转基因的反式激活来完成。这样一种系统已经被报道于麦克布赖德(McBride)等人(1994)美国国家科学院院刊91：7301-7305。
在将异源外源DNA整合到植物细胞中之后，然后在该培养基中应用最大阈值水平的合适选择以杀死这些未转化的细胞，并且通过定期转移到新鲜培养基中来分离和增殖这些从这种选择处理中存活的假定转化的细胞。通过连续传代和使用合适的选择进行攻击，鉴定并增殖了这些用该质粒载体转化的细胞。然后，可以使用分子和生物化学的方法来证实整合到该转基因植物的基因组中的感兴趣的异源基因的存在。
已转化的细胞可以根据常规方法长成植物。参见，例如，麦考密克(McCormick)等人(1986)植物细胞报告(Plant Cell Reports)5：81-84。然后，可以使这些植物生长，并且用相同的转化的品系或不同的品系进行授粉，并且鉴定出这种得到的具有所希望的表型特征的组成型表达的杂合体。可以使两代或更多代生长以确保所希望的表型特征的表达被稳定地维持并遗传，然后收获种子以确保已经获得了所希望的表型特征的表达。按照这种方式，本发明提供了经转化的种子(也被称为“转基因种子”)，该种子具有本发明的核苷酸构建体，例如被稳定地结合到它们的基因组中的本发明的表达盒。
植物转化的评估
在将异源外源DNA引入到植物细胞中之后，通过不同的方法来证实异源基因在植物基因组中的转化或整合，这些方法例如对与该整合的基因相关联的核酸、蛋白以及代谢产物的分析。
PCR分析是在移植到土壤中之前的早期阶段筛选转化的细胞、组织或芽中所结合基因的存在的快速方法(萨姆布鲁克和拉塞尔(2001)分子克隆：实验室手册，冷泉港实验室出版社，冷泉港，纽约)。PCR是使用对感兴趣的基因或土壤杆菌载体背景等特异的寡核苷酸引物类来进行。
可以通过基因组DNA的DNA印迹分析来证实植物类转化(萨姆布鲁克和拉塞尔，2001，同上)。一般而言，从该转化体中提取了总DNA，用合适的限制酶进行消化，在琼脂糖凝胶中进行分级，并且转移至硝酸纤维素或尼龙膜上。然后，根据标准的技术，用例如放射性标记32P的靶标DNA片段来探测该膜或“印迹”以证实引入的基因整合到该植物基因组中(萨姆布鲁克和拉塞尔，2001，同上)。
在RNA印迹分析中，从该转化体的特定组织中分离RNA，在甲醛琼脂糖凝胶中进行分级，并且根据在本领域内是常规的标准操作来印迹到尼龙滤膜上(萨姆布鲁克和拉塞尔，2001，同上)。然后，通过在本领域内已知的方法、通过将该滤膜与衍生自杀有害生物基因的放射性探针进行杂交来测试由该杀有害生物基因所编码的RNA的表达(萨姆布鲁克和拉塞尔，2001，同上)。
使用与存在于杀有害生物蛋白上的一种或多种表位结合的抗体，可以对转基因植物进行蛋白印迹、生物化学测定、以及类似测定，以便通过标准操作来证实由该杀有害生物基因所编码的蛋白的存在(萨姆布鲁克和拉塞尔，2001，同上)。
植物中的杀有害生物活性
在本发明的另一方面，可以产生表达具有杀有害生物活性的杀有害生物蛋白的转基因植物。以上通过实例来说明的方法可用于产生转基因植物，但产生这些转基因植物细胞的方式对于本发明而言不是至关重要的。可以依据实验者的判断来使用在本领域已知或描述的方法，如土壤杆菌介导的转化、生物射弹转化以及非粒子介导法。可以通过在本领域内所说明的普通方法来分离表达杀有害生物蛋白的植物，这些方法例如通过愈伤组织的转化、转化的愈伤组织的选择、以及从此类转基因愈伤组织中再生出能育的植物类。在此类方法中，可以使用任何基因作为选择标记，只要它在植物细胞中的表达赋予了鉴定或选择转化的细胞的能力。
已经开发了多种与植物细胞一起使用的标记物，如对于氯霉素、氨基糖甙G418、潮霉素、以及类似物的抗性。编码参与叶绿体代谢的产物的其他基因也可以用作选择标记。例如，提供对于植物除草剂(如草甘磷、溴苯腈、或咪唑琳酮)的抗性的基因可以具有特别的用途。这样的基因已经被报道(斯托克(Stalker)等人(1985)生物化学杂志263：6310-6314(溴苯腈抗性腈水解酶基因)；以及萨塔西瓦姆(Sathasivan)等人(1990)核酸研究18：2188(AHAS咪唑啉酮抗性基因)。另外，在此披露的这些基因作为评估细菌细胞或植物细胞的转化的标记是有用的。用于检测在植物、植物器官(例如，叶子、茎、根、等)、种子、植物细胞、繁殖体、胚或它们的子代中转基因的存在的方法是本领域内所熟知的。在一个实施例中，通过测试杀有害生物活性来检测转基因的存在。
可以针对杀有害生物活性来测试表达杀有害生物蛋白的能育植物，并且选择显示出最佳活性的植物以用于进一步的育种。在本领域内对有害生物活性进行测定的方法是可供使用的。通常，在摄食测定中混合并使用了这种蛋白。参见，例如马罗内(Marrone)等人(1985)经济昆虫学杂志(J.of Economic Entomology)78：290-293。
本发明可以用于转化任何植物种类，包括但不限于单子叶植物和双子叶植物。感兴趣的植物的实例包括但不限于：玉米(corn)(玉米(maize))、高梁、小麦、向日葵、番茄、十字花科植物、胡椒、马铃薯、棉花、水稻、大豆、甜菜、甘蔗、烟草、大麦、以及油菜、芸苔属植物、苜蓿、黑麦、粟、红花、花生、甘薯、木薯、咖啡、椰子、菠萝、柑桔属的树、可可、茶、香蕉、鳄梨、无花果、番石榴、芒果、橄榄、番木瓜、腰果、澳洲坚果、杏仁、燕麦、蔬菜、观赏性植物、以及针叶树。
蔬菜包括但不限于：番茄、莴苣、青豆、利马豆、豌豆，以及甜瓜属(genusCurcumis)的成员，如黄瓜、网纹甜瓜、以及甜瓜。观赏性植物包括但不限于：杜鹃花属、绣球属、木槿属、蔷薇属、郁金香、水仙属、碧冬茄属、康乃馨、一品红、以及菊花。优选地，本发明的植物是作物(例如玉米、高粱、小麦、向日葵、番茄、十字花科植物、胡椒、马铃薯、棉花、稻、大豆、甜菜、甘蔗、烟草、大麦、油菜籽油菜等)。
在有害生物控制方面的用途
在有害生物控制或工程化处理其他生物中用于采用多种菌株作为杀有害生物剂的总体方法在本领域内是已知的，这些菌株包括本发明的核苷酸序列或它的变体。参见，例如，美国专利号5,039,523和EP 0480762 A2。
包含本发明的核苷酸序列或其变体的芽孢杆菌菌株、或已经被遗传改变而包含本发明的杀有害生物基因和蛋白的微生物可以用于保护农业作物以及产品免受有害生物的侵害。在本发明的一个方面，用以下试剂来处理产生毒素(杀有害生物剂)的生物的完整的(即，未裂解的)细胞，当这些细胞被施用到一种或多种靶标有害生物的环境中时这些试剂延长了在该细胞中产生的毒素的活性。
可替代地，通过将杀有害生物基因引入到细胞宿主中来产生该杀有害生物剂。该杀有害生物基因的表达直接或间接地导致了该杀有害生物剂的细胞内产生和维持。在本发明的一个方面，然后在以下条件下对这些细胞进行处理，当该细胞被施用到一种或多种靶标有害生物的环境时这些条件延长了在该细胞中产生的毒素的活性。得到的产物保留该毒素的毒性。然后，可以按照常规技术来配制这些天然胶囊化的杀有害生物剂，以用于施用到寄宿着靶标有害生物的环境(例如，土壤、水、以及植物的叶)。参见，例如EPA 0192319以及其中所引用的文献。可替代地，可配制表达本发明的基因的这些细胞，例如以便允许得到的材料作为杀有害生物剂的施用。
本发明的活性成分通常以组合物的形式进行施用，并且可以同时或相继地与其他化合物一起施用到有待处理的作物区域或植物中。这些化合物可以是肥料、除草剂、冷冻保护剂(cryoprotectants)、表面活性剂、洗涤剂、杀有害生物肥皂(pesticidal soap)、休眠喷洒油(dormant oil)、聚合物、和/或定时释放或生物可降解载体配制品，这些配制品允许在单次施用该制剂之后对靶标区域进行长期给药。它们还可以是选择性除草剂、化学杀昆虫剂、杀病毒剂、杀微生物剂、杀变形虫剂、杀有害生物剂、杀真菌剂、杀细菌剂、杀线虫剂、杀软体动物剂或这些配制品中的几种的混合物，如果希望的话，与其他农业上可接受的载体、表面活性剂或在配制品领域内通常采用的促进施用的佐剂一起。合适的载体和佐剂可以是固体或液体，并且相应于在配制技术中常常采用的物质，例如天然的或再生的矿物质、溶剂、分散剂、湿润剂、增粘剂、粘合剂或肥料。同样地，可将这些配制品制备成可食用的“诱饵”或塑造成有害生物“陷阱”以允许由靶标有害生物来摄食或摄取该杀有害生物配制品。
施用本发明的活性成分或本发明的农业化学组合物(该组合物包括由本发明的细菌菌株所产生的这些杀有害生物蛋白中的至少一种)的方法包括叶施用、种子涂覆和土壤施用。施用次数以及施用率取决于由相应的有害生物所侵染的强度。
可以将该组合物配制成粉末、粉剂、小丸、颗粒、喷雾剂、乳液、胶体、溶液、或类似剂型，并且可以通过以下常规方法来制备该组合物，这些方法例如将包括该多肽的细菌细胞的培养物干燥、冷冻干燥、均质化、提取、过滤、离心、沉降、或浓缩。在包含至少一种这种杀有害生物多肽的所有此类组合物中，该多肽可以按照按重量计从大约1％至大约99％的浓度而存在。
可以在给定的区域内通过本发明的这些方法来杀死鳞翅目、半翅目、双翅目、或鞘翅目有害生物或减少其数目，或者可以将它们预防性地施用到环境区域内以防止被易受影响的有害生物的侵染。优选地，这种有害生物摄取、或接触杀有害生物有效量的该多肽。“杀有害生物有效量”是指该杀有害生物剂能够引起至少一种有害生物的死亡、或明显减少有害生物的生长、摄食或正常生理发育的量。这个量将取决于以下因素而变化：例如，有待控制的特定的靶标有害生物；有待处理的特定的环境、地点、植物、作物、或农业场所；环境条件；以及施用的杀有害生物有效的多肽组合物的方法、速率、浓度、稳定性、以及数量。这些制剂还可以根据气候条件、环境因素、和/或施用频率和/或有害生物侵染的严重度而变化。
可通过将该细菌细胞、晶体、和/或孢子悬浮物、或分离的蛋白组分与所希望的农业上可接受的载体配制在一起来制备所说明的杀有害生物剂组合物。在施用之前，可以用合适的手段(如冻干、冷冻干燥、干燥的，或在水性载体、介质或合适的稀释剂，如盐水或其他缓冲液中)配制这些组合物。这些经配制的组合物可以处于以下形式：粉剂或颗粒材料、或在油(植物性或矿物性)中的悬浮体、或水或油/水乳液、或作为可湿性粉末、或与适合于农业施用的任何其他载体材料相组合。适合的农业载体可以是固体或液体并且在本领域是熟知的。术语“农业上可接受的载体”覆盖了所有佐剂、惰性组分、分散剂、表面活性剂、增粘剂、粘合剂、等等，它们在杀有害生物剂配制技术中是常用的；这些对于杀有害生物剂配制领域的普通技术人员而言是熟知的。这些制剂可以与一种或多种固体或液体佐剂相混合，并且通过不同的手段进行制备，例如通过使用常规的配制技术将这种杀有害生物组合物与合适的佐剂进行均匀地混合、掺合和/或研磨。合适的制剂和施用方法说明于美国专利号6,468,523中，该文献通过引用结合在此。
“有害生物”包括但不限于：昆虫、真菌、细菌、线虫、螨、蜱、以及类似物。昆虫有害生物包括选自以下目的昆虫：鞘翅目、双翅目、膜翅目、鳞翅目、食毛目、同翅目、半翅目、直翅目、缨翅目、革翅目、等翅目、虱目、蚤目、毛翅目等，特别是鞘翅目、鳞翅目、以及双翅目。
鞘翅目包括肉食亚目(suborder Adephaga)和多食亚目(suborder Polyphaga)。肉食亚目包括步甲总科(superfamily Caraboidea)和豉甲总科(superfamilyGyrinoidea)，而多食亚目包括牙甲总科(superfamily Hydrophi1oidea)、隐翅甲总科(superfamily Staphylinoidea)、花萤总科(superfamily Cantharoidea)、郭公虫总科(superfamily Cleroidea)、叩头虫总科(superfamily Elateroidea)、花甲总科(superfamily Dascilloidea)、泥甲总科(superfamily Dryopoidea)、九甲总科(superfamily Byrrhoidea)、扁甲总科(superfamily Cucujoidea)、芜菁总科(superfamily Meloidea)、花蚤总科(superfamily Mordelloidea)、拟步甲总科(superfamily Tenebrionoidea)、长蝽总科(superfamily Bostrichoidea)、金龟总科(superfamily Scarabaeoidea)、天牛总科(superfamily Cerambycoidea)、叶甲总科(superfamily Chrysomeloidea)以及象甲总科(superfamily Curculionoidea)。步甲总科包括虎甲科(Cicindelidae)、步甲科(Carabidae)、以及龙虱科(Dytiscidae)。豉甲总科包括豉甲科(family Gyrinidae)。牙甲总科包括牙甲科(family Hydrophilidae)。隐翅甲总科包括葬甲科(family Silphidae)和隐翅甲科(family Staphylinidae)。花萤总科包括花萤科(family Cantharidae)和萤科(family Lampyridae)。郭公虫总科包括郭公虫科(C1eridae)和皮蝽科(Dermestidae)。叩头虫总科包括叩头虫科(Elateridae)和吉丁虫科(Buprestidae)。扁甲总科包括瓢虫科(Coccinellidae)。芫菁总科包括芜菁科(Meloidae)。拟步甲总科包括拟步甲科(Tenebrionidae)。金龟总科包括黑蜕科(Passalidae)和金龟科(Scarabaeidae)。天牛总科包括天牛科(Cerambycidae)。叶甲总科包括叶甲科(Chrysomelidae)。象甲总科包括象甲科(Curculionidae)和小蝽科(Scolytidae)。
双翅目包括长角亚目(Nematocera)、短角亚目(Brachycera)、以及环裂亚目(Cyclorrhapha)。长角亚目包括大蚊科(Tipulidae)、毛蠓科(Psychodidae)、蚊科(Culicidae)、蠓科(Ceratopogonidae)、摇蚊科(Chironomidae)、蚋科(Simuliidae)、毛蚊科(Bibionidae)、以及瘿蚊科(Cecidomyiidae)。短角亚目包括水虻科(Stratiomyidae)、虻科(Tabanidae)、剑虻科(Therevidae)、食虫虻科(Asilidae)、拟食虫虻科(Mydidae)、蜂虻科(Bombyliidae)、以及长足虻科(Dolichopodidae)。环裂亚目包括无缝组(DivisionAschiza)和有缝组(Division Schizophora)。无缝组包括蚤蝇科(Phoridae)、蚜蝇科(Syrphidae)和眼蝇科(Conopidae)。无缝组包括无瓣类(Acalyptratae)和有瓣类(Calyptratae)。无瓣类包括斑蝇科(Otitidae)、实蝇科(Tephritidae)、潜蝇科(Agromyzidae)、以及果蝇科(Drosophilidae)。有瓣类包括虱蝇科(Hippoboscidae)、狂蝇科(Oestridae)、寄蝇科(Tachinidae)、花蝇科(Anthomyiidae)、蝇科(Muscidae)、丽蝇科(Calliphoridae)、以及麻蝇科(Sarcophagidae)。
鳞翅目包括凤蝶科(Papilionidae)、粉蝶科(Pieridae)、灰蝶科(Lycaenidae)、蛱蝶科(Nymphalidae)、斑蝶科(Danaidae)、眼蝶科(Satyridae)、弄蝶科(Hesperiidae)、天蛾科(Sphingidae)、天蚕蛾科(Saturniidae)、尺蛾科(Ceometridae)、灯蛾科(Arctiidae)、夜蛾科(Noctuidae)、毒蛾科(Lymantriidae)、透翅蛾科(Sesiidae)以及谷蛾科(Tineidae)。
线虫包括寄生性线虫，例如根结线虫、胞囊线虫、以及根腐线虫，包括异皮线虫属、根结线虫属、以及球异皮线虫属；特别是胞囊线虫的成员，包括但不限于：大豆异皮线虫(Heterodera glycines)(大豆胞囊线虫)；甜菜异皮线虫(甜菜胞囊线虫)；燕麦异皮线虫(Heterodera avenae)(燕麦胞囊线虫)；以及马铃薯金线虫(Globodera rostochiensis)和马铃薯白线虫(Globodera pailida)(马铃薯胞囊线虫)。根腐线虫包括短体线虫属。
半翅目有害生物(其包括指定为半翅目、同翅目、或异翅亚目的物种)包括但不限于草盲蝽属，如西方无泽盲蝽(豆荚草盲蝽)、牧草盲蝽(美国牧草盲蝽)、和绿盲蝽(伊利苏斯草盲蝽(Lygus elisus)；蚜虫，例如碧桃蚜(green peach aphid)(桃蚜(Myzuspersicae))、棉花蚜虫(棉蚜)、樱桃蚜虫或黑樱桃蚜虫(黑樱桃蚜(Myzus cerasi))、大豆蚜虫(大豆蚜(Aphis glycines Matsumura))；褐飞虱(稻褐飞虱)、以及稻青大叶蝉(黑尾叶蝉属)；和蝽象，例如绿色蝽象(喜绿蝽)、棕色大理石纹蝽象(茶翅蝽)、南方绿色蝽象(南方绿椿象)、稻蝽象(美洲稻蝽)、森林红蝽(红足真蝽(Pentatoma rufipes))、欧洲蝽象(砂枣润蝽(Rhaphigaster nebulosa))、以及盾椿象耳蝽(Troilus luridus)。
对于主要农作物的本发明的虫害包括：玉米：玉米螟(Ostrinia nubilalis)、欧洲玉米螟(European corn borer)；小地老虎(Agrotis ipsilon)、黑地老虎(blackcutworm)；谷实夜蛾(Helicoverpa zea)、棉铃虫(corn earworm)；草地夜蛾(Spodopterafrugiperda)、秋夜蛾(fall armyworm)；西南玉米秆草螟(Diatraea grandiosella)、西南玉米螟(southwestern corn borer)；南美玉米苗斑螟(Elasmopalpus lignosellus)，小玉米秆螟(lesser cornstalk borer)；小蔗秆草螟(Diatraea saccharalis)，小蔗秆草螟(surgarcane borer)；玉米根叶甲(Diabrotica virgifera)、西方玉米根螟(western cornrootworm)；长角叶甲巴氏亚种(Diabrotica longicornis barberi)、北方玉米根螟(northern corn rootworm)；黄瓜十一星叶甲食根亚种(Diabrotica undecimpunctatahowardi)，南方玉米根螟(southern corn rootworm)；梳爪叩头虫属(Melanotus spp.)、金针虫(wireworms)；北方圆头犀金龟(Cyclocephala borealis)、北方圆头犀金龟(蛴螬)；南方圆头犀金龟(Cyclocephala immaculata)，南方圆头犀金龟(蛴螬)；日本弧丽金龟(Popillia japonica)，日本金龟子；玉米铜色跳甲(Chaetocnema pulicaria)、玉米跳甲；玉米隐啄象(Sphenophorus maidis)，玉米谷象；玉米缢管蚜(Rhopa1osiphum maidis)，玉米叶蚜虫；玉米根蚜(Anuraphis maidiradicis)，玉米根蚜虫；美洲谷长蝽(Blissusleucopterus leucopterus)，高粱长蝽；赤胫黑蝗(Melanoplus femurrubrum)，红胫蝗虫(redlegged grasshopper)；血黑蝗(Melanoplus sanguinipes)，迁徙蝗虫；玉米种蝇(Hylemya platura)，种蝇；玉米斑潜蝇(Agromyza parvicornis)，玉米斑潜蝇；玉米黄蓟马(Anaphothrips obscrurus)，草蓟马；窃蚁(Solenopsis milesta)，窃蚁；二斑叶螨(Tetranychus urticae)，二斑叶螨；高粱：斑禾草螟(Chilo partellus)，高粱螟；草地夜蛾，秋夜蛾；考斯夜蛾(Spodoptera cosmioides)；南方灰翅夜蛾；谷实夜蛾，棉铃虫；南美玉米苗斑螟，小玉米秆螟；粒肽脏切夜蛾(Feltia subterranea)，颗粒地老虎(granulatecutworm)；长毛食叶然金龟(Phyllophaga crinita)、蛴螬；伪金针虫属(Eleodes spp.)、叩头虫属(Conoderus spp.)、以及Aeolus属，蛴螬；黑角负泥虫(Oulema melanopus)，谷类叶甲；玉米铜色跳甲，玉米跳甲；玉米隐啄象，玉米谷象；玉米缢管蚜，玉米叶蚜；美甘蔗伪毛蚜(Sipha f1ava)，黄甘蔗蚜；美洲谷长蝽，高粱长蝽；高粱瘿蚊(Contarinia sorghicola)，粱痪蚊(sorghum midge)；朱砂叶螨(Tetranychus cinnabarinus)，朱砂叶螨；二斑叶蹒(Tetranychus urticae)，二斑叶蹒；小麦：一点黏虫(Pseuda1etia unipunctata)，黏虫；草地夜蛾，秋夜蛾；南美玉米苗斑螟，小玉米秆螟；西方灰地老虎(Agrotis orthogonia)，西方地老虎；南美玉米苗斑螟，小玉米秆螟；黑角负泥虫，谷类叶甲；三叶草叶象(Hyperapunctata)，三叶草叶象；黄瓜十一星叶甲食根亚种，南方玉米根螟；俄罗斯小麦蚜虫；麦二叉蚜(Schizaphis graminum)，麦二叉蚜；麦长管蚜(Macrosiphum avenae)，麦长管蚜；赤胫黑蝗，赤胫黑蝗；异黑蝗(Melanoplus differentia1is)，异黑蝗；血黑蝗，迁徙蝗虫；小麦瘿蚊(Mayetiola destructor)，小麦瘿蚊(Hessian fly)；麦红吸浆虫(Sitodiplosismosellana)，麦蛆(wheat midge)；美洲杆蝇(Meromyza americana)，美洲麦秆黄潜蝇(wheat stem maggot)；麦疫种蝇(Hylemya coarctata)，麦种蝇(wheat bulb fly)；烟褐花蓟马(Frankliniella fusca)，烟草蓟马；麦茎蜂(Cephus cinctus)，麦茎蜂(wheat stemsawfly)；曲叶螨(Aceria tulipae)，小麦曲叶螨(wheat curl mite)；向日葵：向日葵芽卷叶蛾(Suleima helianthana)，向日葵芽蛾；向日葵同斑螟(Homoeosoma electellum)，向日葵蛾；向日葵叶甲(zygogramma exclamationis)，向日葵甲虫；胡萝卜利犀金龟(Bothyrusgibbosus)，胡萝卜甲虫；向日葵籽瘿蚊(Neolasioptera murtfeldtiana)，向日葵籽蠓；棉 花：烟芽夜蛾(Heliothis virescens)，棉花蚜虫；谷实夜蛾，棉铃虫；甜菜夜蛾(Spodopteraexigua)，甜菜夜蛾；红铃麦蛾(Pectinophora gossypiella)，粉色棉铃虫；棉铃象(Anthonomus grandis)，棉子象鼻虫；棉蚜(Aphis gossypii)，棉花蚜虫；棉序盲蝽(Pseudatomoscelis seriatus)，棉花跳盲蝽；温室粉虱(Trialeurodes abutilonea)，带状翅白粉虱(bandedwinged whitefly)；美国牧草盲蝽(Lygus lineolaris)，tarnishedplant bug；赤胫黑蝗，红胫蝗虫；异黑蝗，异黑蝗；烟蓟马(Thrips tabaci)，洋葱蓟马；烟褐花蓟马，烟蓟马；朱砂叶螨，朱砂叶螨；二斑叶螨，二斑叶螨；水稻：小蔗秆草螟，甘蔗螟；草地夜蛾，秋夜蛾；考斯夜蛾(Spodoptera cosmioides)；南方灰翅夜蛾；谷实夜蛾，棉铃虫；葡萄鞘叶甲(Colaspis brunnea)，葡萄鞘叶甲；稻水象(Lissorhoptrus oryzophilus)，稻水象；米象(Sitophilus oryzae)，米象；二条黑尾叶蝉(Nephotettix nigropictus)，米叶蝉；美洲谷长蝽，高粱长蝽；拟缘蝽(Acrosternum hilare)，绿蝽；二化螟(Chilu suppressalis)，亚洲稻螟；大豆：大豆夜蛾(Pseudoplusia includens)，大豆夜蛾(soybean looper)；梨豆夜蛾，黎豆夜蛾(velvetbean caterpillar)；苜蓿绿夜蛾(Plathypena scabra)，苜蓿绿夜蛾；玉米螟，欧洲玉米螟；小地老虎，黑地老虎；甜菜夜蛾，甜菜夜蛾；考斯夜蛾(Spodopteracosmioides)；南方灰翅夜蛾；烟芽夜蛾，棉花蚜虫；谷实夜蛾，棉铃虫；墨西哥大豆瓢虫(Epilachna varivestis)，墨西哥大豆瓢虫；桃蚜(Myzus persicae)，桃蚜；蚕豆微叶蝉(Empoasca fabae)，马铃薯叶蝉；拟缘蝽，绿蝽；赤胫黑蝗，赤胫黑蝗；异黑蝗，异黑蝗；玉米种蝇，玉米种蝇；大豆蓟马(Sericothrips variabilis)，大豆蓟马；烟蓟马，洋洋葱蓟马；草莓叶螨(Tetranychus turkestani)，草莓叶螨；二斑叶螨，二斑叶螨；大麦：玉米螟，欧洲玉米螟；小地老虎，黑地老虎；麦二叉蚜，麦二叉蚜(greenbug)；美洲谷长蝽，高粱长蝽；拟缘蝽，绿蝽；烟草蝽(Euschistus servus)，茶色蝽；英雄美洲蝽(Euschistus heros)，新热带茶色蝽(neotropical brown stink bug)；灰地种蝇(Delia platura)，种蝇；小麦瘿蚊，小麦瘿蚊；潜岩螨(Petrobia latens)，麦长腿蜘蛛；油籽油菜：甘蓝蚜(Brevicorynebrassicae)，卷心菜蚜；蔬菜黄条跳甲(Phyllotreta cruciferae)，跳甲；蓓带夜蛾(Mamestra configurata)，贝莎夜蛾(Bertha armyworm)；菜蛾(Plutella xylostella)，小菜蛾；地种蝇属(Delia ssp.)，根蛆。
用于增加植物产量的方法
提供了用于增加植物产量的方法。这些方法包括提供表达编码在此披露的杀有害生物多肽序列的多核苷酸的植物或植物细胞并且在被有害生物侵染(或者易受到该有害生物侵染)的田地中使该植物或其种子生长，所述多肽具有针对该有害生物的杀有害生物活性。在一些实施例中，该多肽具有针对鳞翅目、鞘翅目、双翅目、半翅目、或者线虫有害生物的杀有害生物活性，并且所述田地被鳞翅目、半翅目、鞘翅目、双翅目、或线虫有害生物侵染。如本文所定义的，该植物的“产量”指由该植物所产生的生物质的品质和/或数量。“生物质”是指任何经测量的植物产物。生物质产生的增加是在所测量的植物产物的产量方面的任何改进。增加植物产量具有几种商业应用。例如，增加植物叶生物质可以增加用于人或动物消费的叶菜的产量。另外，增加叶生物质可以用于增加植物衍生的药学或工业产物的生产。产量的增加可以包括与不表达该杀有害生物序列的植物相比任何统计学上显著的增加，包括但不限于：产量的至少1％的增加，至少3％的增加，至少5％的增加，至少10％的增加，至少20％的增加，至少30％、至少50％、至少70％、至少100％或更大的增加。在多种特定方法中，植物产量由于植物表达在此披露的杀有害生物蛋白而获得改进的有害生物抗性而增加。该杀有害生物蛋白的表达导致有害生物侵染或摄食的能力减小。
还可以用一种或多种化学成分来处理植物，这些化学成分包括一种或多种除草剂、杀昆虫剂、或杀真菌剂。示例性的化学成分包括：水果/蔬菜类除草剂：莠去津、除草定、敌草隆、草甘膦、利谷隆、嗪草酮、西玛津、氟乐灵、吡氟禾草灵、草铵膦、氯吡嘧磺隆(Gowan)、百草枯、戊炔草胺、烯禾啶、氟丙嘧草酯、氯吡嘧磺隆、三嗪茚草胺(Indaziflam)；水果/蔬菜杀昆虫剂：涕灭威、转苏云金芽孢杆菌(Bacillus thuriengiensis)、甲萘威(Carbaryl)、克百威(Carbofuran)、毒死蜱(Chlorpyrifos)、氯氰菊酯(Cypermethrin)、溴氰菊酯(Deltamethrin)、阿维菌素(Abamectin)、氟氯氰菊酯/β-氟氯氰菊酯、高氰戊菊酯、λ-氟氯氰菊酯、灭螨醌、联苯肼酯、甲氧虫酰肼、双苯氟脲、环虫酰肼、噻虫啉、呋虫胺、嘧螨酯、螺螨酯、γ-氟氯氰菊酯、螺甲螨酯、多杀菌素、氯虫酰胺、氰虫酰胺、杀铃脲、螺虫乙酯、吡虫啉、氟虫酰胺、硫双威(Thiodicarb)、氰氟虫腙、砜虫啶、丁氟螨酯、腈吡螨酯(Cyanopyrafen)、噻虫胺、噻虫嗪、乙基多杀菌素(Spinotoram)、硫双威(Thiodicarb)、氟啶虫酰胺、甲硫威、因灭汀-苯甲酸盐(Emamectin-benzoate)、茚虫威、苯线磷、吡丙醚、苯丁锡氧化物；水果/蔬菜杀真菌剂：唑嘧菌胺、嘧菌酯、苯噻菌胺酯、啶酰菌胺、克菌丹、多菌灵、百菌清、铜、氰霜唑、环氟菌胺、霜脲氰、环丙唑醇、环丙嘧啶、苯醚甲环唑、烯酰吗啉、二噻农、咪唑菌酮、环酰菌胺、氟啶胺、咯菌腈、氟吡菌胺、氟吡菌酰胺、氟嘧菌酯、氟唑菌酰胺(Fluxapyroxad)、灭菌丹、乙膦酸、异菌脲、异丙菌胺、先正达(Isopyrazam)、醚菌酯(Kresoxim-methyl)、代森锰锌、双炔酰菌胺、甲霜灵(Metalaxyl)/精甲霜灵(mefenoxam)、代森联、苯菌酮、腈菌唑、戊菌唑、吡噻菌胺、啶氧菌酯、霜霉威(Propamocarb)、丙环唑、丙森锌、丙氧喹啉、丙硫菌唑、唑菌胺酯、嘧霉胺、喹氧灵、螺环菌胺(Spiroxamine)、硫、戊唑醇、甲基硫菌灵(Thiophanate-methyl)、肟菌酯(Trifloxystrobin)；谷类除草剂：
2.4-D、酰嘧磺隆、溴草腈、唑草酮-E、绿麦隆、氯磺隆、炔草酸-P、二氯吡啶酸、麦草畏、禾草灵-M、吡氟酰草胺、精恶唑禾草灵、双氟磺草胺、氟酮磺隆-NA、氟噻草胺、氟啶嘧磺隆-M、氟草烟(Fluroxypyr)、呋草酮、草甘膦、碘甲磺隆、碘苯腈、异丙隆、MCPA、甲基二磺隆、甲磺隆、二甲戊乐灵、唑啉草酯、丙苯磺隆、苄草丹、啶磺草胺、磺酰磺隆、噻吩磺隆、三甲苯草酮、醚苯磺隆、苯磺隆、氟乐灵、三氟甲磺隆；谷类杀真菌剂：嘧菌酯、联苯吡菌胺(Bixafen)、啶酰菌胺、多菌灵、百菌清、环氟菌胺、环丙唑醇、环丙嘧啶、醚菌胺、氟环唑、苯锈啶、丁苯吗啉、氟吡菌酰胺、氟嘧菌酯、喹唑菌酮、氟唑菌酰胺(Fluxapyroxad)、先正达、醚菌酯、叶菌唑、苯菌酮、吡噻菌胺、啶氧菌酯、咪鲜胺、丙环唑、丙氧喹啉、丙硫菌唑、唑菌胺酯、喹氧灵、螺环菌胺、戊唑醇、甲基硫菌灵、肟菌酯；谷类杀昆虫剂：乐果、λ-三氟氯氰菊酯(Lambda-cyhalthrin)、溴氰菊酯、顺式氯氰菊酯、β-氟氯氰菊酯、联苯菊酯、吡虫啉、噻虫胺、噻虫嗪、噻虫啉、啶虫脒、呋虫胺(Dinetofuran)、毒死蜱(Clorphyriphos)、抗蚜威、甲硫威、砜虫啶；玉米除草剂：莠去津、甲草胺、溴苯腈、乙草胺、麦草畏、二氯吡啶酸、(S-)二甲酚噻草胺、草铵膦、草甘膦、异噁唑草酮、(S-)异丙甲草胺、甲基磺草酮、烟嘧磺隆、氟嘧磺隆、砜嘧磺隆、磺草酮、甲酰胺磺隆、苯吡唑草酮、环磺酮(Tembotrione)、嘧啶肟草醚、酮脲磺草吩酯、氟噻草胺、吡咯磺隆(Pyroxasulfon)；玉米杀昆虫剂：克百威、毒死蜱、联苯菊酯、氟虫腈、吡虫啉、λ-氯氟氰菊酯、七氟菊酯、特丁磷、噻虫嗪、噻虫胺、螺甲螨酯、氟虫酰胺、杀铃脲、氯虫酰胺、溴氰菊酯、硫双威、β-氟氯氰菊酯、氯氰菊酯、联苯菊酯、氯芬奴隆(Lufenuron)、丁基嘧啶磷、乙虫腈、氰虫酰胺、噻虫啉、啶虫脒、呋虫胺、阿维菌素；玉米杀真 菌剂：嘧菌酯、联苯吡菌胺、啶酰菌胺、环丙唑醇、醚菌胺、氟环唑、种衣酯、氟吡菌酰胺、氟嘧菌酯、氟唑菌酰胺、先正达、叶菌唑、吡噻菌胺、啶氧菌酯、丙环唑、丙硫菌唑、唑菌胺酯、戊唑醇、肟菌酯；水稻除草剂：丁草胺、敌稗、四唑嘧磺隆、苄嘧磺隆、氰氟草酯、杀草隆、四唑酰草胺、咪唑磺隆、苯噻草胺、去稗安、吡嘧磺隆、稗草畏、二氯喹啉酸、禾草丹、茚草酮、氟噻草胺、四唑酰草胺、氯吡嘧磺隆、去稗安、苯并双环酮、环酯草醚、五氟磺草胺、双草醚、稻思达、乙氧嘧磺隆、丙草胺、硝草酮、特糠酯酮(Tefuryltrione)、恶草酮、精恶唑禾草灵、吡丙醚(Pyrimisulfan)；水稻杀昆虫剂：二嗪农、仲丁威、丙硫克百威、噻嗪酮、呋虫胺、氟虫腈、吡虫啉、异丙威、噻虫啉、环虫酰肼、噻虫胺、乙虫腈、氟虫酰胺、氯虫酰胺、溴氰菊酯、啶虫脒、噻虫嗪、氰虫酰胺、多杀菌素、乙基多杀菌素、因灭汀-苯甲酸盐、氯氰菊酯、毒死蜱、醚菊酯、克百威、丙硫克百威、砜虫啶；水稻杀真菌剂：嘧菌酯、多菌灵、环丙酰菌胺、双氯氰菌胺、苯醚甲环唑、敌瘟磷、嘧菌腙、庆大霉素、己唑醇、恶霉灵、异稻瘟净(IBP)、稻瘟灵、异噻菌胺、春日霉素(Kasugamycin)、代森锰锌、苯氧菌胺、肟醚菌胺、戊菌隆、噻菌灵、丙环唑、丙森锌、咯喹酮、戊唑醇、甲基硫菌灵、噻酰菌胺、三环唑、肟菌酯、有效霉素(Validamycin)；棉花除 草剂：敌草隆、伏草隆、MSMA、乙氧氟草醚、扑草净、氟乐灵、唑草酮、烯草酮、丁基吡氟禾草灵、草甘膦、哒草伏、二甲戊乐灵、嘧硫苯甲酸钠、三氟啶磺隆、得杀草、草铵膦、丙炔氟草胺、塞苯隆；棉花杀昆虫剂：乙酰甲胺磷、涕灭威、毒死蜱、氯氰菊酯、溴氰菊酯、阿维菌素、啶虫脒、因灭汀-苯甲酸盐、吡虫啉、茚虫威、λ-氯氟氰菊酯、多杀菌素、硫双威、γ-氯氟氰菊酯、螺甲螨酯、啶虫丙醚、氟啶虫酰胺、氟虫双酰胺、杀铃脲、氯虫酰胺、β-氟氯氰菊酯、螺虫乙酯、噻虫胺、噻虫嗪、噻虫啉、呋虫胺、氟虫双酰胺、氰虫酰胺、多杀菌素、乙基多杀菌素、γ氯氟氰菊酯、4-[[(6-氯吡啶-3-基))甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮、硫双威、阿维菌素、氟啶虫酰胺、啶虫丙醚、螺甲螨酯、砜虫啶；棉花杀真菌剂：嘧菌酯、联苯吡菌胺、啶酰菌胺、多菌灵、百菌清、酮、环丙唑醇、苯醚甲环唑、醚菌胺、氟环唑、咪唑菌酮、氟啶胺、氟吡菌酰胺、氟嘧菌酯、氟唑菌酰胺、异菌脲、先正达、异噻菌胺、代森锰锌、代森锰、苯氧菌胺、吡噻菌胺、啶氧菌酯、甲基代森锌、丙硫菌唑、唑菌胺酯、五氯硝基苯、戊唑醇、氟醚唑、甲基硫菌灵、肟菌酯；大豆除草剂：甲草胺、灭草松、氟乐灵、氯嘧磺隆、氯酯磺草胺、精恶唑禾草灵、氟磺胺草醚、丁基吡氟禾草灵、草甘膦、甲氧咪草烟、灭草喹、咪草烟、(S-)异丙甲草胺、嗪草酮、二甲戊乐灵、得杀草、草铵膦；大豆杀昆虫剂：λ-氟氯氰菊酯、灭多虫、吡虫啉、噻虫胺、噻虫嗪、噻虫啉、啶虫脒、呋虫胺、氟虫酰胺、氯虫酰胺、氰虫酰胺、多杀菌素、乙基多杀菌素、因灭汀-苯甲酸盐、氟虫腈、乙虫腈、溴氰菊酯、β-氟氯氰菊酯、γ和λ氯氟氰菊酯、4-[[(6-氯吡啶-3-基))甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮、螺虫乙酯、螺螨酯、杀铃脲、氟啶虫酰胺、硫双威、β-氟氯氰菊酯；大豆杀真菌剂：嘧菌酯、联苯吡菌胺、啶酰菌胺、多菌灵、百菌清、酮、环丙唑醇、苯醚甲环唑、醚菌胺、氟环唑、氟啶胺、氟啶胺、氟吡菌酰胺、氟嘧菌酯、粉唑醇、氟唑菌酰胺、先正达、异菌脲、异噻菌胺、代森锰锌、代森锰、叶菌唑、苯氧菌胺、腈菌唑、吡噻菌胺、啶氧菌酯、丙环唑、甲基代森锌、丙硫菌唑、唑菌胺酯、五氯硝基苯、戊唑醇、氟醚唑、甲基硫菌灵、肟菌酯；甜菜除草剂：杀草敏、甜安宁、乙氧氟草黄、甜菜宁、野麦畏、二氯吡啶酸、丁基吡氟禾草灵、环草定、苯嗪草酮、喹草酸、噻草酮、三氟啶磺隆、得杀草、精喹禾灵；甜菜杀昆虫剂：吡虫啉、噻虫胺、噻虫嗪、噻虫啉、啶虫脒、呋虫胺、溴氰菊酯、β-氟氯氰菊酯、γ/λ氯氟氰菊酯、4-[[(6-氯吡啶-3-基))甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮、七氟菊酯、氯虫酰胺、氰虫酰胺、氟虫腈、克百威；卡诺拉(Canola)除草剂：二氯吡啶酸、禾草灵、丁基吡氟禾草灵、草铵膦、草甘膦、吡草胺、氟乐灵、胺苯磺隆、喹草酸、精喹禾灵、烯草酮、得杀草；卡诺拉杀真菌剂：嘧菌酯、联苯吡菌胺、啶酰菌胺、多菌灵、环丙唑醇、苯醚甲环唑、醚菌胺、氟环唑、氟啶胺、氟啶胺、氟吡菌酰胺、氟嘧菌酯、氟硅唑、氟唑菌酰胺、异菌脲、先正达、甲哌鎓氯、叶菌唑、苯氧菌胺、多效唑(Paclobutrazole)、吡噻菌胺、啶氧菌酯、咪鲜胺、丙硫菌唑、唑菌胺酯、戊唑醇、甲基硫菌灵、肟菌酯、烯菌酮；卡诺拉杀昆虫剂：克百威、噻虫啉、溴氰菊酯、吡虫啉、噻虫胺、噻虫嗪、啶虫脒、呋虫胺(Dinetofuran)、β-氟氯氰菊酯、γ-和λ-氯氟氰菊酯、τ-氟胺氰菊酯(Fluvaleriate)、乙虫腈、多杀菌素、乙基多杀菌素、氟虫酰胺、氯虫酰胺、氰虫酰胺(Cyazypyr)、4-[[(6-氯吡啶-3-基)甲基](2,2-二氟乙基)氨基]呋喃-2(5H)-酮。
将本发明的基因引入到另一种植物中的方法
此处还提供了将本发明的核酸引入到另一种植物中的方法。本发明的核酸或其片段可通过轮回选择、回交、谱系育种、纯系选择、混合选择、突变育种和/或遗传标记物增强的选择被引入第二种植物中。
因此，在一个实施例中，本发明的方法包括使包括本发明的核酸的第一植物与第二植物杂交以产生F1子代植物，并选择包括本发明的核酸的F1子代植物。该方法可以进一步包括使所选择的子代植物与包括本发明的核酸的第一植物杂交以产生回交子代植物，并选择包括本发明的核酸的回交的子代植物。在本文其他地方提供了用于评估杀有害生物活性的方法。这些方法可以进一步包括继将这些步骤重复一次或多次之后产生所选择的包括本发明的核酸的第二或更高回交的子代植物。
包括针对植物所希望的表型进行选择的任何育种方法都可以用在本发明的方法中。在一些实施例中，这些F1植物可以自花授粉从而产生分离F2代。然后可以在每代(F3、F4、F5等)中选择代表了所希望的表型(例如，杀有害生物活性)的个体植物，直到这些性状纯合或固定在育种种群中。
该第二植物可以是具有所希望的性状的植物，该性状如除草剂耐受性、昆虫耐受性、耐旱性、线虫控制、水分利用效率、氮利用效率、改进的营养价值、抗病性、改进的光合作用、改进的纤维质量、胁迫耐受性、改进的繁殖等。该第二植物可以是如在本文其他处描述的原种事件。
在不同的实施例中，植物部分(整个植物、植物器官(例如，叶、茎、根等)、种子、植物细胞、繁殖体、胚等)可以从所得杂交收获并且被繁殖或收集用于下游用途(例如作为食品、饲料、生物燃料、油、面粉、粕(meal)等)。
获得植物产品的方法
本发明还涉及用于获得商品的工艺，包括收获和/或碾磨来自包括本发明的核酸的作物的籽粒，以获得该商品。包括但不限于以下项的农艺学和商业上重要的产品和/或物质的组合物旨在处于本发明的范围内：预期用作人类消费的食品或用于在旨在用于人类消费的组合物和商品中使用的动物饲料、商品、和植物产品以及副产品，特别是失活种子/籽粒产品，包括产生自此类籽粒/种子的(半-)加工产物，其中所述产物是或包括全种子或籽粒或经处理的种子或籽粒、动物饲料、玉米或大豆粕、玉米或大豆粉、玉米、玉米淀粉、大豆粕、大豆粉、薄片、大豆蛋白浓缩物、大豆蛋白分离物、组织化大豆蛋白浓缩物、化妆品、护发产品、大豆坚果黄油、纳豆、印尼豆豉(tempeh)、水解大豆蛋白、植脂奶油(whippedtopping)、起酥油、卵磷脂、可食用全大豆(原始、烘焙、或作为毛豆)、大豆酸奶、大豆干酪、豆腐、腐竹，连同烹饪的、精制的、汽蒸的、烘焙的或被煮半熟的籽粒等等，如果这些产品和物质的组合物含有可检测量的在此列出的核苷酸和/或氨基酸序列(如针对含有此类核苷酸序列的任何植物所诊断的)的话。
通过说明而不是通过限制来提供以下实例。
实验性实例
实例1.从苏云金芽孢杆菌中发现新颖杀有害生物基因
使用以下步骤从表1中列出的细菌菌株中鉴定新颖的杀有害生物基因：
·从该菌株中制备总的DNA。总的DNA含有基因组DNA和染色体外DNA两者。染色体外的DNA包括以下各项的一些或全部的混合物：不同尺寸的质粒、噬菌体染色体、其他未表征的染色体外分子。
·对该DNA进行测序。通过新一代测序方法对总的DNA进行测序。
·通过同源性和/或其他计算分析来鉴定推定的毒素基因。
·当需要时，通过几种PCR或克隆策略中的一种(例如TAIL-PCR)来对感兴趣的基因进行序列最终处理。
表1.从细菌菌株中鉴定的新颖基因
*表示自下游起始位点编码的蛋白，并且“(截短)”表明该蛋白具有C-末端截短。
实例2.表达和纯化
使用II融合DNA聚合酶与结合3'末端处的AscI接头的引物，从表1中列出的相应菌株PCR扩增编码在表2中列出的氨基酸序列中每个的基因。用AscI消化扩增的PCR产物并将其连接到pMalC4X载体中。通过测序证实克隆并且将质粒转化到Bl21感受态细胞中。将单一菌落接种到LB培养基中并在37℃下生长直到到达对数期，并且在20℃下使用0.5mM IPTG持续诱导18小时。在室温下以1:50比率用Xa因子(Factor Xa)将纯化的蛋白消化过夜。根据标准方案，将纯化的蛋白提交到对选定昆虫有害生物进行的生物测定。结果在以下表2中示出。有害生物列于表3中，并且评分系统在表3之后。
表2.生物测定结果(矮化，死亡率得分)
表3.生物测定组中的昆虫
Ae 黄热病蚊子 埃及伊蚊
BCW 黑地老虎 小地老虎
CA 棉花蚜 棉蚜
DBM 菱形斑蛾 小菜蛾
ECB 欧洲玉米螟 玉米螟
FAW 秋夜蛾 草地夜蛾
Hv 烟夜蛾 烟芽夜蛾
Hz 棉铃虫 谷实夜蛾
SBA 大豆蚜虫 大豆蚜
SBL 大豆夜蛾 大豆夜蛾
SCB 甘蔗螟虫 小蔗秆草螟
SWCB 西南玉米螟 西南玉米秆草螟
VBC 黎豆夜蛾 梨豆夜蛾
矮化得分：
0＝无活性
1＝不均匀矮化
2＝轻度均匀矮化(75％-100％对照的尺寸)
3＝强均匀矮化(74％-26％之间对照的尺寸)
4＝严重均匀矮化(小于25％对照的尺寸)
死亡率得分：
0＝无活性
1＝高达25％死亡率
2＝高达50％死亡率
3＝高达75％死亡率
4＝大于75％死亡率
实例3.杀有害生物活性的其他测定
本发明的核苷酸序列可以针对它们产生杀有害生物蛋白的能力来进行测试。经常通过多种方式来评定杀有害生物蛋白充当针对有害生物的杀有害生物剂的能力。在本领域内熟知的方法是进行一项摄食测定。在这项摄食测定中，将这种有害生物暴露于包括有待测试的化合物的样品或对照样品。经常，这是通过将有待测试的材料、或此类材料的合适的稀释物置于该有害生物将摄取的材料(如，人工饵料)上来进行。有待测试的材料可以由液体、固体、或浆料组成。可将有待测试的材料置于表面上，并接着允许其干燥。可替代地，可将有待测试的材料与溶化的人工饵料进行混合，并且随后将它们分配到测定小室中。测定小室可以是例如杯子、皿、或微量滴定板的孔。
用于吸吮性有害生物(sucking pest)(例如，蚜虫)的测定可以涉及通过分隔物将测试材料与昆虫分开，这种分隔物理想地是可以被这种吸吮性昆虫的吸吮式口器刺穿以便允许摄取这种测试材料的部分。经常将这种测试材料与摄食刺激物(如蔗糖)相混合，以便促进该测试化合物的摄取。
其他类型的测定可以包括将这种测试材料显微注射到这种有害生物的口或肠内，以及形成转基因的植物，之后测试这种有害生物摄食该转基因植物的能力。植物测试可涉及分离被正常地消耗的植物部分，例如放在叶上的小笼，或分离在包括昆虫的笼中的整个植物。
测试有害生物的其他方法和手段在本领域是已知的，并且可见于例如罗伯森(Robertson)和普雷斯勒(Preisler)编著(1992)使用节肢动物的杀有害生物剂生物测定(Pesticide bioassays with arthropods)，CRC，波卡拉顿(Boca Raton)，佛罗里达州(FL)。可替代地，在期刊节肢动物管理测试(Arthropod Management Tests)以及经济昆虫学杂志中、或通过与美国昆虫学学会(Entomological Society of America，ESA)的会员的讨论一般性地描述了这些测定。
在一些实施例中，将编码在此披露的这些杀有害生物蛋白的毒素区域的DNA区域克隆到大肠杆菌表达载体pMAL-C4x中，位于编码麦芽糖结合蛋白(MBP)的malE基因后面。这些框内融合物导致大肠杆菌内的MBP-Axmi融合蛋白表达。
对于大肠杆菌内的表达，用多个单独的质粒转化BL21*DE3。将多个单一菌落接种到补充有羧苄青霉素和葡萄糖的LB中，并且在37℃下生长过夜。次日，用1％的过夜培养物接种新鲜的培养基并且于37℃下生长至对数生长期。随后，在20℃下用0.3mM IPTG诱导培养物。将每个细胞沉淀悬浮于20mM Tris-Cl缓冲液、pH 7.4+200mM NaCl+1mM DTT+蛋白酶抑制剂中并且进行超声处理。由SDS-PAGE进行的分析可以用于证实融合蛋白的表达。
随后，将总的无细胞提取物上样于连接至快速蛋白液相色谱(FPLC)的直链淀粉柱中，以亲和纯化MBP-axmi融合蛋白。用10mM麦芽糖溶液将结合的融合蛋白从树脂中洗脱出来。随后用Xa因子或胰蛋白酶切割纯化的融合蛋白，以便从Axmi蛋白中除去氨基末端MBP标签。可以通过SDS-PAGE确定蛋白的切割和溶解度
实例4.用于植物表达的基因的运载
本发明的编码区是与用于在植物中表达的适当的启动子和终止子序列相连的。这样的序列在本领域是熟知的，并且可以包括用于在单子叶植物中表达的水稻肌动蛋白启动子或玉米泛素启动子，用于在双子叶植物中表达的拟南芥属UBQ3启动子或CaMV 35S启动子以及nos或PinII终止子。用于生产并证实启动子-基因-终止子构建体的技术在本领域内也是熟知的。
在本发明的一个方面，设计并产生了合成的DNA序列。这些合成序列具有相对于亲本序列有所改变的核苷酸序列，但是编码与亲本序列基本一致的蛋白。
在本发明的另一方面，设计这些合成基因的修饰变体以使得将得到的肽靶向植物细胞器，如内质网或质外体。已知导致融合蛋白靶向植物细胞器的肽序列在本领域是已知的。例如，来自白羽扇豆的酸性磷酸酶基因的N末端区域(ID GI：14276838，米勒等人(2001)植物生理学(Plant Physiology)127：594-606)在本领域是已知导致异源蛋白的内质网靶向的。如果得到的融合蛋白在C-末端还包含内质网保留序列，该内质网保留序列包括肽N-末端-赖氨酸-天冬氨酸-谷氨酸-亮氨酸(即，“KDEL”基序，SEQ ID NO：:75)，则该融合蛋白将靶向内质网。如果这种融合蛋白在C末端缺少靶向内质网的序列，则该蛋白将被靶向该内质网，但最终将被隔离在质外体中。
因此，这个基因编码融合蛋白，该融合蛋白包含来自白羽扇豆(IDGI：14276838，米勒等人，2001，同上)的酸性磷酸酶基因的N-末端三十一个氨基酸，它们与本发明的氨基酸序列的N-末端相融合，以及包括在C-末端处的KDEL(SEQ ID NO：75)序列。因此，预测得到的蛋白一旦表达于植物细胞中时就被靶向到该植物的内质网。
将以上说明的植物表达盒与合适的、辅助转化的细胞和组织的选择的植物可选择标记相组合，并且将其连接到植物转化载体中。这些可以包括来自土壤杆菌介导的转化的二元载体或用于气溶胶或生物射弹转化的简单的质粒载体。
实例5.大豆转化
大豆转化是通过使用本领域中熟知的方法实现的，例如如下描述的方法：使用根瘤土壤杆菌介导的转化大豆半种子外植体，基本上使用由帕斯(Paz)等人(2006)，植物细胞报告(Plant cell Rep.)25：206所述的方法。使用环磺酮作为选择标记鉴定转化体。观察到绿芽的外观，并记录为耐除草剂异噁唑草酮或环磺酮的指示物。这些耐受性转基因芽将显示可比得上与未用异噁唑草酮或环磺酮处理的野生型大豆绿芽的正常绿化，而用相同量的异噁唑草酮或环磺酮处理的野生型大豆芽将被完全漂白。这表明HPPD蛋白的存在使得对HPPD抑制剂除草剂如异噁唑草酮或环磺酮的耐受性成为可能。
耐受的绿芽被转移到生根培养基或进行嫁接。在驯化周期后，生根的小植株被转移到该温室。然后将含有该转基因的植物用HPPD抑制剂除草剂喷雾，如例如用环磺酮以100g AI/公顷的比率或用甲基磺草酮以300g AI/公顷的比率(补充有硫酸铵甲酯菜籽油)。在施用后十天，对由于施用了该除草剂而产生的症状进行评价，并且与在野生型植物上在相同条件下观察到的症状相对比。
实例6：棉花T0植物建立和选择。
棉花转化是通过使用本领域中熟知的方法进行的，尤其是在PCT专利公开WO 00/71733中描述的优选方法。将再生的植物转移到温室中。在驯化周期后，将充分生长的植物用HPPD抑制剂除草剂喷雾，如例如用补充有硫酸铵和甲酯菜籽油的等于100或200g AI/公顷的环磺酮。在施用喷雾后七天，对归因于用该除草剂处理而产生的症状进行评价，并且与经受相同处理的野生型棉花植物在相同条件下观察到的症状相对比。
实例7.使用在此说明的杀有害生物蛋白基因进行的玉米细胞的转化
在授粉后8至12天收集玉米穗。将胚与穗进行分离，并且那些大小为0.8-1.5mm的胚是优选用于转化的。将胚以盾片侧向上的方式置于在适当的孵育培养基中，如DN62A5S培养基(3.98g/L N6盐；1mL/L(的1000x母液)N6维生素类；800mg/L L-天冬酰胺；100mg/L肌醇；1.4g/L L-脯氨酸；100mg/L酪蛋白氨基酸；50g/L蔗糖；1mL/L(的1mg/mL母液)2,4-D)。然而，除DN62A5S以外的培养基和盐类是适合的并且在本领域内是已知的。将胚在黑暗中于25℃下孵育过夜。然而，本质上不需要将这些胚孵育过夜。
将所得到的外植体转移至网眼方阵(30-40个/平板)，转移至渗透培养基上并保持约30-45分钟，随后转移至聚束平板(beaming plate)上(参见，例如，PCT公开号WO/0138514和美国专利号5,240,842)。
在植物细胞中将被设计为本发明基因的DNA构建体加速进入植物组织中，这是使用气溶胶束加速器、使用基本上如PCT公开号WO/0138514中所说明的条件进行的。在聚束之后，将胚在渗透培养基上孵育约30分钟，并且将其置于孵育培养基上于25℃下黑暗中过夜。为了避免不适当地破坏经聚束的外植体，它们在转移至恢复培养基之前被孵育至少24小时。然后，将胚在25℃下在黑暗中扩散到恢复培养基上，持续大约5天，随后转移至选择培养基中。将外植体在选择培养基中孵育达到8周，这取决于所利用的特定选择的性质和特征。在选择期之后，将得到的愈伤组织转移至胚成熟培养基中，直至观察到成熟的体细胞胚的形成。然后，将得到的成熟的体细胞胚置于低光照下，并且通过本领域内已知的方法来引发再生过程。允许这些得到的芽在生根培养基上生根，并且将得到的植物转移至育苗盆(nursery pot)中并繁殖成转基因植物。
材料
DN62A5S培养基
用1N KOH/1N KCl将该溶液的pH调整至pH 5.8，加入高达3g/L的浓度的结冷胶(Gelrite)(西格玛公司)，并将该培养基高压灭菌。在冷却至50℃之后，加入2ml/L的5mg/ml硝酸银母液(植物技术实验室(Phytotechnology Labs))。
实例8.通过土壤杆菌介导的转化将本发明的基因转化到植物细胞中
穗最好在授粉之后8至12天进行收集。将胚与穗进行分离，并且那些大小为0.8-1.5mm的胚是优选用于转化的。将胚以盾片侧向上的方式置于适合的孵育培养基中，并且在25℃下在黑暗中孵育过夜。然而，本质上不需要将这些胚孵育过夜。将胚与土壤杆菌属菌株进行接触，该菌株包含用于Ti质粒介导的转移的适当的载体，持续约5-10min，并且然后将其置于共培养基上持续约3天(22℃下、在黑暗中)。共培养之后，将外植体转移到恢复期培养基中，持续5-10天(25℃下、在黑暗中)。将外植体在选择培养基中孵育达到8周，这取决于所利用的特定选择的性质和特征。在选择期之后，将得到的愈伤组织转移至胚成熟培养基中，直至观察到成熟的体细胞胚的形成。然后，将得到的成熟的体细胞胚置于低光照下，并且如本领域内所已知地引发再生过程。
实例9.水稻的转化
自温室中良好受控条件下生长的供体植物收集含有正确发育阶段的胚的未成熟水稻种子。在将种子灭菌后，将未成熟的胚切除并且在固体培养基上前诱导3天。在前诱导后，将胚在包含所希望的载体的土壤杆菌悬浮液中浸没几分钟。然后将胚在包含乙酰丁香酮的固体培养基上共培养并且在黑暗中孵育4天。然后将外植体转移到含有作为选择性试剂的草铵膦的第一选择培养基中。在大约3周之后，将发展了愈伤组织的小盾片切成几个较小的片并且将其转移到相同的选择培养基中。随后大约每2周进行继代培养。在每次继代培养后，将活跃生长的愈伤组织切成较小的片并且在第二选择培养基上孵育。在若干周之后，将明确抗草铵膦的愈伤组织转移到选择再生培养基。将产生的小植株培养在半强度MS上用于完全延伸。最终将这些植株转移到土壤中并且使其在温室中生长。
在本说明书中提到的所有公开文件和专利申请对于本发明所涉及的领域的普通技术人员的技术水平而言是指示性的。所有公开物和专利申请均通过引用结合在此，其程度如同每个单独的公开物或专利申请被确切地并单独地指明通过引用而被结合。
尽管本发明在上文中出于理解清楚的目的、通过展示和实例已经相当详细地进行说明，应当清楚是可以在所附的权利要求书的范围内实行某些改变和修饰。
序列表
<110> 艾斯尼克斯公司（Athenix Corp.）
拜耳作物科学公司（Bayer CropScience LP）
赛耶, 丽贝卡（Thayer, Rebecca）
罗伯茨, 基拉（Roberts, Kira）
桑普森, 金柏莉（Sampson, Kimberly）
莱赫蒂宁, 杜安（Lehtinen, Duane）
彼得斯, 谢丽尔（Peters, Cheryl）
马加良斯, 李奥纳多（Magalhaes, Leonardo）
邓恩, 伊桑（Dunn, Ethan）
<120> 毒素基因及其使用方法
<130> 2916693-20178WO01
<150> 61/774,110
<151> 2013-03-07
<150> 61/774,627
<151> 2013-03-08
<150> 61/774,629
<151> 2013-03-08
<150> 61/774,635
<151> 2013-03-08
<150> 61/774,638
<151> 2013-03-08
<150> 61/774,642
<151> 2013-03-08
<150> 61/774,645
<151> 2013-03-08
<150> 61/774,647
<151> 2013-03-08
<150> 61/774,650
<151> 2013-03-08
<150> 61/774,655
<151> 2013-03-08
<150> 61/774,659
<151> 2013-03-08
<160> 75
<170> PatentIn版本3.5
<210> 1
<211> 2382
<212> DNA
<213> 苏云金芽孢杆菌
<400> 1
atgacttgtc aattacaagc gcaaccactt attccctata acgtactagc aggagttcca 60
actagtaata caggtagtcc aatcggcaat gcaggtaatc aatttgatca gtttgagcaa 120
accgttaaag agctcaagga agcatgggaa gcgttccaaa aaaacggaag tttctcatta 180
gcagctcttg aaaagggatt tgatgcagca atcggaggag gatcctttga ttatttaggt 240
ttagttcaag ccggcctagg attagttggt acgctaggcg ccgcaatccc tggtgtttca 300
gtggcagtgc ctcttattag catgcttgtt ggtgtttttt ggccaaaggg cacaaacaac 360
caagaaaacc ttattacagt tattgataag gaagttcaga gaatactaga tgaaaagcta 420
tctgatcagt taataaagaa attgaacgca gatttaaatg cttttacgga cctagtaact 480
cgtttggaag aagtaataat agatgcaact ttcgagaatc acaagcctgt actacaagta 540
agtaaatcaa attatatgaa agtggattca gcatatttct caacaggagg tattcttact 600
cttggcatga gtgattttct tactgatacc tattcaaagc ttaccttccc attatatgta 660
ctaggcgcaa ctatgaaact ttcagcatat catagttata tacaattcgg aaatacatgg 720
cttaataaag tttatgattt atcatcagat gagggaaaaa caatgtcgca ggctttagca 780
cgagctaaac agcatatgcg ccaagacata gcattttata caagccaagc tttaaacatg 840
tttactggga atctcccttc attatcatct aataaatatg caattaatga ctataatgta 900
tacactcgag caatggtatt gaatggctta gatatagtag caacatggcc taccctatat 960
ccagatgact attcgtctca gataaaactg gagaaaacac gcgtgatctt ttcagatatg 1020
gtcgggcaaa gtgagagtag agatggcagc gtaacgatta aaaatatttt tgacaataca 1080
gattcacatc aacatggatc cataggtctc aattcaatct cttatttccc agatgagtta 1140
cagaaagcac aacttcgcat gtatgattat aatcacaaac cttattgtac ggactgtttc 1200
tgctggccgt atggagtgat tttaaactat aacaagaata cctttagata tggcgataat 1260
gatccaggtc tttcaggaga cgttcaactc ccagcaccta tgagtgtagt taatgcccaa 1320
actcaaacag cccaatatac agatggagaa aacatatgga cagatactgg ccgcagttgg 1380
ctttgtactc tacgtggcta ctgtactaca aactgttttc caggaagagg ttgttataat 1440
aatagtactg gatatggaga aagttgcaat caatcacttc caggtcaaaa aatacatgca 1500
ctatatcctt ttacacaaac aaatgtgctg ggacaatcag gcaaactagg attgctagca 1560
agtcatattc catatgacct aagtccgaac aatacgattg gtgacaaaga tacagattct 1620
acgaatattg tcgcaaaagg aattccagtg gaaaaagggt atgcatccag tggacaaaaa 1680
gttgaaatta tacgagagtg gataaatggt gcgaatgtag ttcaattatc tccaggccaa 1740
tcttggggaa tggattttac caatagcaca ggtggtcaat atatggtccg ctgtcgatat 1800
gcaagtacaa acgatactcc aatctttttt aatttagtgt atgacggggg atcgaatcct 1860
atttataacc agatgacatt ccctgctaca aaagagactc cagctcacga ttcagtagat 1920
aacaagatac taggcataaa aggaataaat ggaaattatt cactcatgaa tgtaaaagat 1980
tctgtcgaac ttccatctgg gaaatttcat gtttttttca caaataatgg atcatctgct 2040
atttatttag atcgacttga gtttgttcct ttaggaaaac catcccctgg tgttttatat 2100
tctggttctt atgatcttat gggatcacag tatgctagcg ttctttttaa cgaccaaaat 2160
gcctcctata ccacagtttc tataaatggg gtatctgatg cacatagtac ctcaggaagt 2220
attactcttt ttaataatga gacattagta aaaggatttg atgtaccagg ttcaggtcaa 2280
agttatcaat attctaatgt aactgtgcct ccttataata gagtcaatat gacgaaaggc 2340
acctatgctg aactttcagg ttctgtaaca attaaaggaa at 2382
<210> 2
<211> 3519
<212> DNA
<213> 苏云金芽孢杆菌
<400> 2
gtgctaaaat taagaaaacg gaggtatttt atggagggaa ataatctaaa tcaatgcata 60
ccttacaatt gtttaagtaa tcctaaggac ataatattag gtgatgaaag gctagaaact 120
ggtaatactg tagcagacat taccttaggg attgtcaatc tattgttttc tgagtttgtt 180
cctggtggag gctttatact aggattactg gatttaatat gggggtctat aggtcgttcc 240
caatgggatc tatttctgga acagattgaa caattgatta agcaaagaat agaagaattt 300
gctaggaatc aggcaatttc aaggttggag gggctaagcg atctttataa gacctatgct 360
agagcgttta gcgattggga ggcagatccg actaatccag cattaaggga agaaatgcgt 420
atacaattta atgacatgaa tagtgctatc ataacggctc tcccactttt tagagttcaa 480
aattatgaag ttgctctttt atctgtttat gttcaagctg caaacttaca tttatctatt 540
ttaagagatg tttcagtctt tggagaaaga tggggatatg atacagcaac tatcaataat 600
cgctatagtg acttaactag ccttattcat gtttatacta atcattgtgt ggatacgtat 660
aatcagggat taaggcgttt ggaaggtcgt tttcttaccg attggattgt atataatcgt 720
ttccgaagac aattaacaat ttcagtatta gatattgttg cattttttcc aaattatgat 780
attcgaacat atccaattca aacagctact cagctaacga gggaaatcta tctggattta 840
ccttttatta atgaaaatct ttctcctgca gcaagctatc cctcattctc agatgctgaa 900
agtgctataa tcaggagtcc tcatttagtg gactttttaa atagcttcac tatttataca 960
gatagtcttg ctcgatattt atattgggga gggcatcggg tgaattttac ccgttcagga 1020
gttactactt ttatacaatc accactatat ggaagggaag gaaatgcaga gcgttctgta 1080
attatttcgg catcatctag cgtaccaata tttagaacac tttcatatgt tactggcctt 1140
gacaatgcaa atcctgtagc tggaattgaa ggagtggaat tccaaaatac tataagtaga 1200
agtatctatc gtaaaagtgg tccaattgat tcttttaatg aattaccacc tcaagatgcc 1260
agtgtatctc cttcaattgg gtatagtcac cgtttatgtc atgccacatt tttagaacgg 1320
attagtggac caagaattgc aggtgtcgtt ttttcctgga cacatcgtag tgctagccct 1380
actaatgaag taagttcatc tagaattaca caaattccat gggtaaaggc gcatactctt 1440
gcgtctggtg cctccgttat taaagggcct ggatttacag gtggagatat actaactagg 1500
aataccttag gcgaactggg aactttaaga gtaacttttg caggaagatt atcacaaagt 1560
tattatatac gtttccgtta tgcttccgta gctaatagga gtggtatatt tagctattca 1620
cagccaactt catatggaat ttcctttcca aaaactatgg atgcaaatga atcattaaca 1680
tctcgttcat ttgcacttgc tacacttgct acaccgctaa cctttagaag gcaagaagaa 1740
ttaaatctac aaataccatc aggtacttat atagatcgaa ttgagtttgt tccagtcgat 1800
gaaaccttta caacagaatc tgatctggat agagcacaac aggcggtgaa tgcgctgttt 1860
acttcttcca atcaaatcgg cttaaaaaca gatgtgacgg attatcatat tgatcaagta 1920
tccaatttag tggattgttt atcggatgaa ttttgtctgg atgaaaaaaa agaattgtcc 1980
gagaaagtca aacatgcgaa gcgacttagt gatgagcgga atttacttca agatccaaac 2040
tttagaggga tcaatagaca actagaccgt ggctggagtg gaagtacgga tattaccatc 2100
caaggaggag atgacgtatt caaagagaat tacgttacac taccaggtac ctttgatgag 2160
tgctatccaa cgtatttata tcaaaaaata gatgagtcga aattaaaagc ctatacccgt 2220
tatcaattaa gagggtatat cggggatagt caagacttag aaatctattt aattcgttac 2280
aatgcaaaac acgaaatagt aaatgtacca ggtacaggga gtttatggcc tctttctgta 2340
gaaaattcaa ttggaccttg tggagaaccg aatcgatgcg cgccacacct tgaatggaat 2400
cctaatctag agtgttcttg cagagaaggg gaaaaatgtg cccatcattc ccatcatttc 2460
tccttggaca ttgatgttgg atgtacagac ttaaatgagg acttaggtgt atgggcgata 2520
ttcaagatta agacgcaaga tggccatgca agactaggaa atctagagtt tctcgaagag 2580
aaaccattag taggggaagc actagctcgt gtgaaaagag cggagaaaaa atggagagac 2640
aaacgtgaaa aattggaatt ggaaacaaat attgtttata aagaggcaaa agaatctgta 2700
gatgctttat ttgtaaactc tcaatatgat agattacaag cggataccaa catcgcgatg 2760
attcatgcgg cagataaacg cgttcatagc attcgagaag catatcttcc agagttatct 2820
ataattccgg gtgtaaatgc gggcattttc gaagaattag agggacgtat ttacacagcc 2880
tactctctat atgatgcgag aaatgtcatt aaaaatggcg atttcgataa tggcttatta 2940
tgctggaacg tgaaagggca tgtagatgta gaagaacaaa acaatcaccg ttcagttctg 3000
gttatcccag aatgggaagc agaagtgtca caagaagttc gtgtctgtcc aggacgtggc 3060
tatatccttc gtgttacagc gtacaaagag ggatatggag agggctgcgt aacgatccat 3120
gagatcgaag acaatacaga cgaactgaaa ttcagcaact gtgtagaaga ggaagtatat 3180
ccaaacaaca cggtaacgtg taatgattat actgcgactc aagaagaata tgagggtacg 3240
tacacttctc gtaatcgagg atatgacgga gcctatgaaa gcaattcttc tgtaccagct 3300
gattatgcat cagcctatga agaaaaagcg tatacagatg gaagaagaga gaatccttgt 3360
gaatctaata gaggatatgg ggattacgcg ccactaccag ctggttatgt gacaaaggaa 3420
ttagagtact tcccagaaac cgataaggta tggattgaga tcggagaaac ggaaggaaca 3480
ttcattgtgg atagtgtgga attactcctt atggaggaa 3519
<210> 3
<211> 1767
<212> DNA
<213> 苏云金芽孢杆菌
<400> 3
atggagggaa ataatctaaa tcaatgcata ccttacaatt gtttaagtaa tcctaaggac 60
ataatattag gtgatgaaag gctagaaact ggtaatactg tagcagacat taccttaggg 120
attgtcaatc tattgttttc tgagtttgtt cctggtggag gctttatact aggattactg 180
gatttaatat gggggtctat aggtcgttcc caatgggatc tatttctgga acagattgaa 240
caattgatta agcaaagaat agaagaattt gctaggaatc aggcaatttc aaggttggag 300
gggctaagcg atctttataa gacctatgct agagcgttta gcgattggga ggcagatccg 360
actaatccag cattaaggga agaaatgcgt atacaattta atgacatgaa tagtgctatc 420
ataacggctc tcccactttt tagagttcaa aattatgaag ttgctctttt atctgtttat 480
gttcaagctg caaacttaca tttatctatt ttaagagatg tttcagtctt tggagaaaga 540
tggggatatg atacagcaac tatcaataat cgctatagtg acttaactag ccttattcat 600
gtttatacta atcattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660
tttcttaccg attggattgt atataatcgt ttccgaagac aattaacaat ttcagtatta 720
gatattgttg cattttttcc aaattatgat attcgaacat atccaattca aacagctact 780
cagctaacga gggaaatcta tctggattta ccttttatta atgaaaatct ttctcctgca 840
gcaagctatc cctcattctc agatgctgaa agtgctataa tcaggagtcc tcatttagtg 900
gactttttaa atagcttcac tatttataca gatagtcttg ctcgatattt atattgggga 960
gggcatcggg tgaattttac ccgttcagga gttactactt ttatacaatc accactatat 1020
ggaagggaag gaaatgcaga gcgttctgta attatttcgg catcatctag cgtaccaata 1080
tttagaacac tttcatatgt tactggcctt gacaatgcaa atcctgtagc tggaattgaa 1140
ggagtggaat tccaaaatac tataagtaga agtatctatc gtaaaagtgg tccaattgat 1200
tcttttaatg aattaccacc tcaagatgcc agtgtatctc cttcaattgg gtatagtcac 1260
cgtttatgtc atgccacatt tttagaacgg attagtggac caagaattgc aggtgtcgtt 1320
ttttcctgga cacatcgtag tgctagccct actaatgaag taagttcatc tagaattaca 1380
caaattccat gggtaaaggc gcatactctt gcgtctggtg cctccgttat taaagggcct 1440
ggatttacag gtggagatat actaactagg aataccttag gcgaactggg aactttaaga 1500
gtaacttttg caggaagatt atcacaaagt tattatatac gtttccgtta tgcttccgta 1560
gctaatagga gtggtatatt tagctattca cagccaactt catatggaat ttcctttcca 1620
aaaactatgg atgcaaatga atcattaaca tctcgttcat ttgcacttgc tacacttgct 1680
acaccgctaa cctttagaag gcaagaagaa ttaaatctac aaataccatc aggtacttat 1740
atagatcgaa ttgagtttgt tccagtc 1767
<210> 4
<211> 1965
<212> DNA
<213> 苏云金芽孢杆菌
<400> 4
atgacccaca atgacaacaa taataaattc gaaatcaagg atactggtac caagccaagg 60
tccccccttg caaatgcacc aggtcctaca tggcaaaata taaataatag agatgtagag 120
acatttggat caatagaaat tgcgggaaaa gtagtttcag gcgttataag ctcagttata 180
aaatctttaa gacaacaagc gcaaatagat aaaatagtag caatcgtcgt ggttgaagtc 240
tttgaagtgc tttggcctgt cctagaaggt atgtggtatg cgatgatgga tgctgtagaa 300
attatgattc aggaagccat tacaactgcg gtaagaagta aagcacaagc agaattaaat 360
ggcattcgca atgcccttgt actctttcaa caagctttcg acgactggga aaaaaattca 420
gataatccac aattacaaga tcgtgtaaga aggcaattta ctgcgacaaa tacattaatt 480
caattcgcca tgtcttcctt tgcagttcca ggttttcaag tcccattact cgttgtatat 540
gctcaagcgg ccaacctaca tttactgttt ttacgagagg ctgttgtact tggagaaaag 600
tgggggatga gtcgagaaga ggtagatgat tattataatg gagaacttgg actaactgag 660
ctcacacaat catacacgaa tcactgcaca aattggtatc atgagggatt agcgcaatca 720
atgaaattaa acccgagtgt aacaatacta gaacagtgga atttatataa tgactttaga 780
agagaaatga cgattatgat cttagatatt gtagcgttat ggccgacata tgatgtcaag 840
ttatatcccg caggaaccaa aacagagctc acacgaataa tatatacgcc attaatggga 900
gttttggaag atagtagttc tataagtgcc acaaggaaag aatatggtaa tatagcggct 960
ataaatcagc aagatggagc tacgacgatt ccaccagcat tatttatatg gttggagaaa 1020
caaattgtat atccgtataa caatctcata tatagttatc aaaatttcca aaaaaccaca 1080
tttggtaaag tgatgaatgg cactatattc ggaagtaata gcgagaaaca cctaacaaat 1140
cccataagta ttccgattga tgcagagtca tatgatgttt ataaagttga tacatcatat 1200
tcagcaaaga tggaaattaa aagttcacca attcataaac tagtatacta tcgctcaaaa 1260
gaacaacaag aaagtataat cagtaccaat acatcaaaag gtccaattga tcaggtgtct 1320
gaaatagcaa atgaaggata tcaagattat agtcattgtc ttgctcatat ggcggggtgg 1380
gtatcgattg cttacgggac gggtctgacc gagaagcctt atttagtccc atataattta 1440
gcactaggat ggacgtatgc aaatgttgat cccgtgaata gtatagcacc cgatgcaata 1500
acccaaattc cagcagtgaa aggggataaa gtaattggta ttccagaaga agaggctatt 1560
ttgggcgagg taactgcgat acaaggacct gggtttacag gaggaaattt agtaggattg 1620
ttcgctggtg ctgaattaca tatgaaagtt acaaatccag taagtaatgt tgcaggatat 1680
caaatgagaa ttcgttatgc gaacaatcac ccaacgatac ttgctgtcag ctatcaaggt 1740
gtagaaacat cctcaggtaa atttgacgtt ccagttacgt attctggtga ttttaaaact 1800
aagctaacat ataatgcctt taaatttaaa gaagctatta ttataccttc accattaaga 1860
gaagagattg ctgatatagt attgcgtaat gaaggtgata gcaatctcct tatcgataaa 1920
attgaactca tcccaatgga tttttggagg catgagcaat gcaat 1965
<210> 5
<211> 3717
<212> DNA
<213> 苏云金芽孢杆菌
<400> 5
atgaatcaac agcataacaa tgaatatgaa atcatgagta ctggcgacat gggctatcag 60
ccaaggtatc ccttttcgaa tgcacctggt gctgaattgc aacagatgca ttacaaagat 120
tggatggata tgtgtgcaga tggagagtcg ggaaaaacgt ttgcagacct tacagttcag 180
gagggagtca ctatagctgt aagtattgca gcggcaattc ttagtgtacc atttccagta 240
acagcagcgg gattaagtat tatctcttta ctagttccat actggtggcc agagacagca 300
gtaactcccg gtactccctc tgcgcaagtt acatgggaaa aatttatgag tgctgcggaa 360
aatctcagta atacacaaat tgtagcgagt aaacgatcag atgccattgc tagatggcaa 420
ggtatacaaa ccttaggaag agactatttt caagcgcaat gtgactggct acaagatcaa 480
aataatgaac tcaaaaaaag taaattacgg gaggcatttg atgacttcga agattattta 540
aaagtgtcaa tgccattctt tcgtgcgcaa ggctttgaaa ttcctatgtt agctatgtat 600
gcacaagctg cgaatatgca cttacttttg ctaagagagg ttgtccagaa tggtgtgggt 660
tgggggttcc agcaatacga agttgaccga tattattcca atacagaccc ttttttaggg 720
aaccctggac tattacaact attagaagga tatacggact attgtgtaaa atggtataat 780
gcaggtttac gacaacaata cgaaaataat agatacaatt gggatgcatt caatgatttc 840
cgtagagata tgattataat ggtattagat attgtatctt tatggccaac ttatgatccg 900
aagcgctatc ccctacctac aaaatcacaa cttacacgaa ctgtgtatac cgatttagta 960
ggtttttctg gaaatagcga gtacctacaa atagacattg aacgtgcaga acaagcgcta 1020
gtccagaaac ctggtctatt tacctggctg cgtgagctaa gtttcgagct ggggccactg 1080
tcgcgaatta attttgtaag aggaagacaa atagtcttca attataccgg aagttccgat 1140
aggtatgaag agaccaaggg gaatcttgga gaaactagag aaactgttgt tattccggca 1200
ccggatgtag gggatgacat ctggaggatt agcactcaag ttaacaccta tcagatacca 1260
aatgctactt ttgttagagg ctggaatttt tcttttactc aatcattaga tcaaaaaata 1320
gcttggcgga cagaatattc acccgaaata gtgatgcagg gattgtcttg tcacggacct 1380
tctgtgagtt cttgtaacct ttgtatcagt aacagtccgt gtagaagtat tactcctaac 1440
tatagctccc cttgtgatga caagttggtt tatagtcatc gattttcata tttaggagcc 1500
ggacttaaat ccgatttaac aacgttgatt tattttagct acggatggac ccatgtaagc 1560
gcagatgcaa acaatttgat agatcctaaa aagattaccc aaatcccagc cgtaaagggg 1620
gattatttag ggagaaatgc ccgtgttata aaaggacctg gaagtacagg tggagatcta 1680
gtccaacttt ccgatggaac cgaaagagga actctgggca tcaaactaac aaaaccacca 1740
ggaagtcaca gctatcgtgt aagaatacgt tatgcaagta atacgcgaac tcaacttgag 1800
attatttggg gagaagatta cgatagcgtt atagttcctg ctactacaac tgatataaca 1860
aatctcacct ataataaatt cgggtacttc gaaatccgcg tttttagtta taattcatca 1920
agcgaagaag aagacttaat aagagtggat gctacgggtt ctttcatcct cgacaaaatc 1980
gaattcattc caattgaggg atcagtggat gaatatcaag caaatcagga tctagaaaaa 2040
gcaaaaaagg ccgtgaatgc cttgtttaca ggtgatgcga aaagtgcgct aaaattgagc 2100
atcacaggct acatagtgga tcaagctgcc aactttgtgg aatgtgtatc agatgaattc 2160
catgcccaag aaaaaatgat cctattggat caagtgaaat tcgcgaaacg actgagtcaa 2220
gcacgcaatc tattaaacta tggggatttt gaatcctcag attggtcggg agagaatgga 2280
tggagaacca gtcctcatgt ccatgtggca tctaataatc caatctttaa agggcgctat 2340
ctccacatgc caggtgcgat gagccctcaa ttctctaaca atacctatcc aacgtatgcg 2400
tatcaaaaag tggatgagtc aaaattaaag tcctataccc gttacctcgt acgtggactt 2460
gttggaaata gtaaagatct agaattactg gtggaacgat atggaaaaga tgtacatgtg 2520
gaaatggatg taccaaatga tattcaatat acattaccaa cgaatgactg tggtggcttt 2580
gatcgatgta aaccagtatc gtatcaaaca ggaacttctt cgtacaaatc gtgtggatgc 2640
aagaacaacg acacgtatca gaatggaatg catctatcta aatcatgtgg ttgcaaaaaa 2700
gatccacatg tcttcaccta ccatattgac acaggatgcg tggatcaaga agaaaactta 2760
ggtctattct ttgcattaaa aatcgcaagt gaaaatggta tggcgaacat tgataacctg 2820
gaaatcattg aggcacagcc gttaaaaggg gaagccttgg ctcgtgtgaa aaaacgagaa 2880
cagaaatgga aacaggaaat ggcgcaaaaa cttttacgaa cagagaaagc tgtacaagca 2940
gcgaaagatg cactgcagac tctattcaca aacgcgcagt acaatcgtct caaatttgaa 3000
accctgttcc cacaaattgt ccatgcagag aaactcgtac agcagatccc atatgcgtac 3060
catccattct tgagcgggac gctgtcaact gtaccaggta tgaattttga aatcatccaa 3120
caactattgg cagtgattgg aaatgcccgt acattatacg agcaacgaaa tctcttgcgt 3180
actggtacat tcagctcagg taccggaagt tggaaagtga cagaaggtgt aaaggtgcag 3240
ccactgcaag acacatctgt tctggttctg tcggaatgga gtcatgaagc gtcccagcag 3300
ttacacatgg atccagatcg cggatatgta ttacgtgtaa cagcgcgaaa agaaggcgga 3360
ggaaaaggga ctgtcaccat gagtgactgt gcagactaca cggagacact gacctttaca 3420
tcatgtgact ataacacgta tggttcccaa acgatgacaa gtggtacatt atctggattt 3480
gtgacgaaga cgttagaaat tttcccagat acggatcgga ttcgaattga tattggggaa 3540
accgaaggaa cgttccaagt agaaagtgtg gaattgattt gtatggaaca gatggaggac 3600
gacttatata atatggcggg gaacgtggcg gaagagatgc aagttctaca gcaatctcgt 3660
tccggtagcc acacattaga tcctttatgt aacacaagaa ttggcgagtt cgattgt 3717
<210> 6
<211> 1995
<212> DNA
<213> 苏云金芽孢杆菌
<400> 6
atgaatcaac agcataacaa tgaatatgaa atcatgagta ctggcgacat gggctatcag 60
ccaaggtatc ccttttcgaa tgcacctggt gctgaattgc aacagatgca ttacaaagat 120
tggatggata tgtgtgcaga tggagagtcg ggaaaaacgt ttgcagacct tacagttcag 180
gagggagtca ctatagctgt aagtattgca gcggcaattc ttagtgtacc atttccagta 240
acagcagcgg gattaagtat tatctcttta ctagttccat actggtggcc agagacagca 300
gtaactcccg gtactccctc tgcgcaagtt acatgggaaa aatttatgag tgctgcggaa 360
aatctcagta atacacaaat tgtagcgagt aaacgatcag atgccattgc tagatggcaa 420
ggtatacaaa ccttaggaag agactatttt caagcgcaat gtgactggct acaagatcaa 480
aataatgaac tcaaaaaaag taaattacgg gaggcatttg atgacttcga agattattta 540
aaagtgtcaa tgccattctt tcgtgcgcaa ggctttgaaa ttcctatgtt agctatgtat 600
gcacaagctg cgaatatgca cttacttttg ctaagagagg ttgtccagaa tggtgtgggt 660
tgggggttcc agcaatacga agttgaccga tattattcca atacagaccc ttttttaggg 720
aaccctggac tattacaact attagaagga tatacggact attgtgtaaa atggtataat 780
gcaggtttac gacaacaata cgaaaataat agatacaatt gggatgcatt caatgatttc 840
cgtagagata tgattataat ggtattagat attgtatctt tatggccaac ttatgatccg 900
aagcgctatc ccctacctac aaaatcacaa cttacacgaa ctgtgtatac cgatttagta 960
ggtttttctg gaaatagcga gtacctacaa atagacattg aacgtgcaga acaagcgcta 1020
gtccagaaac ctggtctatt tacctggctg cgtgagctaa gtttcgagct ggggccactg 1080
tcgcgaatta attttgtaag aggaagacaa atagtcttca attataccgg aagttccgat 1140
aggtatgaag agaccaaggg gaatcttgga gaaactagag aaactgttgt tattccggca 1200
ccggatgtag gggatgacat ctggaggatt agcactcaag ttaacaccta tcagatacca 1260
aatgctactt ttgttagagg ctggaatttt tcttttactc aatcattaga tcaaaaaata 1320
gcttggcgga cagaatattc acccgaaata gtgatgcagg gattgtcttg tcacggacct 1380
tctgtgagtt cttgtaacct ttgtatcagt aacagtccgt gtagaagtat tactcctaac 1440
tatagctccc cttgtgatga caagttggtt tatagtcatc gattttcata tttaggagcc 1500
ggacttaaat ccgatttaac aacgttgatt tattttagct acggatggac ccatgtaagc 1560
gcagatgcaa acaatttgat agatcctaaa aagattaccc aaatcccagc cgtaaagggg 1620
gattatttag ggagaaatgc ccgtgttata aaaggacctg gaagtacagg tggagatcta 1680
gtccaacttt ccgatggaac cgaaagagga actctgggca tcaaactaac aaaaccacca 1740
ggaagtcaca gctatcgtgt aagaatacgt tatgcaagta atacgcgaac tcaacttgag 1800
attatttggg gagaagatta cgatagcgtt atagttcctg ctactacaac tgatataaca 1860
aatctcacct ataataaatt cgggtacttc gaaatccgcg tttttagtta taattcatca 1920
agcgaagaag aagacttaat aagagtggat gctacgggtt ctttcatcct cgacaaaatc 1980
gaattcattc caatt 1995
<210> 7
<211> 3618
<212> DNA
<213> 苏云金芽孢杆菌
<400> 7
ttgaattggt taagtaaaaa atgcttatct actttaaatg taaacttgta taaatcacaa 60
ttccaggggg aatatatgga taacaatagt gaaaaccaat gcgtccctta taattgttta 120
agtaatctag aggagataac attgaatggg gaaagactct caacgaatag caccccaatt 180
aatatttcca tgtctgtttc aaagtttctc ctgactgaac ttattccagg tttagggttt 240
gtatttggat tacttgatgc aatatggggg tttataggac ctgatcaatg gacggaattt 300
attgaacata ttgaagaatt aataggtcaa agaataacag ttgtagtaag aaatacagca 360
attcgagaat tggaaggaat ggcacgcgtt tatcaaacct atgctactgc gtttgcggca 420
tgggaaaagg atcctaataa tccggaatta agagaggcat tacgtgcaca atttactgca 480
accgagacct atataagtgg aaggatatct gttttgacaa ttgaggatta ccaggtacaa 540
ctgttatctg tctatgctca agctacaaat ttacatttat ctttattaag agatgttgtg 600
ttttggggtc agaggtgggg cttttcgaca acgactttaa ataattatta tagtgattta 660
actagagaga ttaatgaata taccaattat gctgtccatt ggtacaatgt aggattagaa 720
caattacagg gaccaagctt tcaagagtgg gtcgcataca atcgctatag aagggaatta 780
acactaactg tattggatat tgttactctt ttccataatt atgatatcag gttgtaccca 840
atcccaacta ttagtcaact aacaagggaa gtctatacag acccaatagt tagtggaata 900
gggcagccta acagttggga ttttcctacc ttctcagaag cagaaaataa gtcaattaga 960
acccctcatc tgatggattt cttgaggaac ctcacgattt ttacagattc ggcccggtat 1020
ggtgcaatat accatttctg gggagggcat caaatatcct ctagccttgt agggggaagt 1080
aatataacat ttcccacgta tgggagtaat gtgtcccaag ggagtccttg gatattagtc 1140
acaaatggaa ttccaatata taggacatta tcaaatccct attacaggtt ccttttccaa 1200
tcagttggta gcgcccgttt acgttgtgtt ttgggtgtac aatttcacat ggataatcgt 1260
gcctttacgt atcgcgaaaa ggggacagtg gattcctttg atgaattacc acctacggat 1320
gcaagtgtgt cacctagtga aggatatagc caccgtttat gtcatgcaac actttttcaa 1380
gtaagaaccg gcgggggtgg ggctgtaagc ttttctagaa cagatggagt agtcttttcc 1440
tggacgcatc gtagtgcaac tcctacaaat acaattgatc caaatgttat tactcaaatt 1500
cctgcggtaa agggaagatc tctttttaat ggtgcagtaa ttaaaggacc aggatttact 1560
ggtggagatt tagttagatt aaataggaat aatggtaata ttcaaaatag aggtcatctt 1620
ccaattccaa tccaattctc gtcgcgttct accagatatc gagttcgttt acgttatgct 1680
tctgcaaccc caattcaagt caatgttcat tgggaaaata gcacaatttt ttcaggtata 1740
gtaccagcta cggctcagtc attagataaa ctacaatcaa acgattttgg ttactttgag 1800
atcgctaata ctatttcatc ttcattagat ggtatagtag gtattagaaa ttttagtgca 1860
aatgcagatt tgataataga cagatttgaa tttatcccag tgaatgcaac ctccgaggca 1920
gaatatgatt tagaaagagc gcaagaggcg gtgaatgctc tgtttacttc tacgaatcaa 1980
agaggactta aagcgaatgt aacagattat tacattgatc aagtatccaa tctagtagaa 2040
tgcctatcgg atgagttctg cctagatgaa aagagagaat tatccgagaa agtcaaacag 2100
gcgaagcgaa tcagtgatga gcggaatttg ctccaggatt caaacttcag atgtatcaat 2160
ggtgaagaag accgtggatg gagaggaagt acgcatatta ccatccaagg aggaaacgat 2220
gtattcaaga gaaattttgt tacattacca ggtgcctttg atgcgtgtta tccaacgtat 2280
ttgtatcaaa gaatagatga atcgaaatta aaagcctata cacgctataa attaagagga 2340
tatatagaag atagtcaaga cttggagatt tatttgatcc gttacaatgc gaaacatgaa 2400
acagtaaatg taccaggtac tgagtcccta tggtcgcttt gtactgagag cccaattgga 2460
acgtgtggag aaccaaatcg atgcgcacca caaattgaat ggaatcctga tctaaattgt 2520
tcctgcaaag ccggagaaaa atgtgcacat cattcccatc atttctcctt ggacattgat 2580
gttggatgta cagacttaaa tgaggactta ggtgtatggg tgatattcaa gattaagacg 2640
caggatggcc atgcaagatt aggaaatcta gagtttctcg aagagaaacc gttattagga 2700
gaagcgttag ctcgtgtgaa aagagctgag aaaaaatgga gagacaaacg tgaaaaattg 2760
cagtttgaaa cgaaaatcgt ttacaaagag gcaaaagaat ctgtagatgc tttattcgta 2820
gattctcaat ataatagatt acaagcggat acgaacatta cgatgattca tgcggcagat 2880
aaacgcgttc atcgaatccg agaagcgtat ctgccagagt tgtctgtgat tccgggtgtc 2940
aatgcggcta ttttcgaaga attagaaggt cttattttca ctgcattctc cttatatgat 3000
gcgagaaatg tgataaaaaa cggagatttc aataatggtt tatcatgctg gaacgtaaaa 3060
gggcatgtag atgtacaaca gagccatcat cgttctgtcc ttgttctccc agaatgggaa 3120
gcagaagtgt cacaagaagt tcgtgtctgt ccaggtcgtg gctacatcct tcgtgttaca 3180
gcttacaaag aaggatatgg agaaggatgc gtaacgattc atgagattga gaatcatact 3240
gaaaaattga agtttagaaa ctgtgaagaa gaggacatct atccaaccaa tacggtaacg 3300
tgtcatgatt ataatgtgaa tcaaggcgca gaaggatgcg cagatacatg taattcacgt 3360
catcgtggat atgacgaaac ctatggaaat gattcttccg tatcagctga ttatatgcca 3420
gtttatgagg aagaagtata tacagatgga cgaagagata atccttgtga aatggaaaga 3480
ggttacacac ctttaccagt tgattatgtg acaaaagaat tagaatactt ccctgaaaca 3540
aatacagtat ggattgagat tggagaaacg gaaggaacat tcatcgtaga cagcgtggaa 3600
ttactcctta tggaagaa 3618
<210> 8
<211> 1827
<212> DNA
<213> 苏云金芽孢杆菌
<400> 8
atggataaca atagtgaaaa ccaatgcgtc ccttataatt gtttaagtaa tctagaggag 60
ataacattga atggggaaag actctcaacg aatagcaccc caattaatat ttccatgtct 120
gtttcaaagt ttctcctgac tgaacttatt ccaggtttag ggtttgtatt tggattactt 180
gatgcaatat gggggtttat aggacctgat caatggacgg aatttattga acatattgaa 240
gaattaatag gtcaaagaat aacagttgta gtaagaaata cagcaattcg agaattggaa 300
ggaatggcac gcgtttatca aacctatgct actgcgtttg cggcatggga aaaggatcct 360
aataatccgg aattaagaga ggcattacgt gcacaattta ctgcaaccga gacctatata 420
agtggaagga tatctgtttt gacaattgag gattaccagg tacaactgtt atctgtctat 480
gctcaagcta caaatttaca tttatcttta ttaagagatg ttgtgttttg gggtcagagg 540
tggggctttt cgacaacgac tttaaataat tattatagtg atttaactag agagattaat 600
gaatatacca attatgctgt ccattggtac aatgtaggat tagaacaatt acagggacca 660
agctttcaag agtgggtcgc atacaatcgc tatagaaggg aattaacact aactgtattg 720
gatattgtta ctcttttcca taattatgat atcaggttgt acccaatccc aactattagt 780
caactaacaa gggaagtcta tacagaccca atagttagtg gaatagggca gcctaacagt 840
tgggattttc ctaccttctc agaagcagaa aataagtcaa ttagaacccc tcatctgatg 900
gatttcttga ggaacctcac gatttttaca gattcggccc ggtatggtgc aatataccat 960
ttctggggag ggcatcaaat atcctctagc cttgtagggg gaagtaatat aacatttccc 1020
acgtatggga gtaatgtgtc ccaagggagt ccttggatat tagtcacaaa tggaattcca 1080
atatatagga cattatcaaa tccctattac aggttccttt tccaatcagt tggtagcgcc 1140
cgtttacgtt gtgttttggg tgtacaattt cacatggata atcgtgcctt tacgtatcgc 1200
gaaaagggga cagtggattc ctttgatgaa ttaccaccta cggatgcaag tgtgtcacct 1260
agtgaaggat atagccaccg tttatgtcat gcaacacttt ttcaagtaag aaccggcggg 1320
ggtggggctg taagcttttc tagaacagat ggagtagtct tttcctggac gcatcgtagt 1380
gcaactccta caaatacaat tgatccaaat gttattactc aaattcctgc ggtaaaggga 1440
agatctcttt ttaatggtgc agtaattaaa ggaccaggat ttactggtgg agatttagtt 1500
agattaaata ggaataatgg taatattcaa aatagaggtc atcttccaat tccaatccaa 1560
ttctcgtcgc gttctaccag atatcgagtt cgtttacgtt atgcttctgc aaccccaatt 1620
caagtcaatg ttcattggga aaatagcaca attttttcag gtatagtacc agctacggct 1680
cagtcattag ataaactaca atcaaacgat tttggttact ttgagatcgc taatactatt 1740
tcatcttcat tagatggtat agtaggtatt agaaatttta gtgcaaatgc agatttgata 1800
atagacagat ttgaatttat cccagtg 1827
<210> 9
<211> 2370
<212> DNA
<213> 苏云金芽孢杆菌
<400> 9
gtgaaaaaga tgaattctta tcaaaataag aatgaatatg aaatactgga tgcttcagaa 60
aatactgtaa atgcgttaaa cagatatcct ttcgcaaata atccgtattc ttccattttt 120
agttcttgtc cacgcagtgg gcctggtaat tggattaata tactaggaaa tgcagttagc 180
gaagcagtat ctatttcgca agatataata tctcttctta cacagccttc tatctctggg 240
ataatttcaa tggcatttag tcttttaagt agaatgatag gtagtaatgg aaggtctata 300
tcggagttat ctatgtgtga cttactagct attattgatt tgcgggtaaa tcagagtgtt 360
ttggatgacg gagttgctga ttttaacggc tcgttagtta tatacagaaa ctatttagag 420
gctttacaaa ggtggaacaa taatcccaat cccgctaatg ccgaagaggt tcgtactcgt 480
tttagggaat ctgatacaat attcgatctc attttaacac aagggtcttt aacgaatggc 540
ggttcattag ccagaaataa tgctcaaata ttattattgc cttcttttgc aaatgctgca 600
tactttcatt tactgctatt aagagatgct aatgtatatg ggaataattg gggtttattt 660
ggggttacac ctaatataaa ttatgaatcg aaattactaa accttattag attatatact 720
aattattgca cacattggta taatcaagga ctaaatgaac taagaaatcg aggttccaat 780
gctacagctt ggttggaatt tcatagattt cgtagagata tgacattgat ggtattggat 840
atagtatcat cattttcaag tcttgatatt actagatatc caagagcaac agattttcaa 900
ttgagtagga taatttatac agatccaatt ggttttgtaa atcgtagtga ccctagcgca 960
ccaagaacct ggtttagttt tcacaatcaa gctaattttt cagcgttaga aagtggaata 1020
cctagtcctt cattctcaca attcttagat agtatgcgta tatctactgg cccgcttagt 1080
ttacctgctt ctcctaatat ccatagagca cgggtatggt atggtaatca aaataacttt 1140
aatggatcta gtagccaaac ttttggggaa ataacaaatg ataatcaaac catttcgggt 1200
ttaaatattt tcagaataga ttcacaggct gttaatctaa ataatactac gtttggagtt 1260
agtagagctg aattttatca tgatgctagt caaggctctc aaagatccat atatcaagga 1320
tttgttgata caggtggggc tagtaccgct gtagcccaga atattcaaac atttttcccg 1380
ggagaaaatt cgagtatacc aactccacaa gattatactc atatattaag taggtcaaca 1440
aatttaacag gaggacttcg acaagtagca tctggacgtc gttcttcttt agtattacac 1500
ggttggacac ataaaagtct gagtcgtcaa aatagagttg aaccaaatag aattactcaa 1560
gtgccggctg ttaaagcaag ttctccttcg aattgtactg taattgcagg acctggattt 1620
acaggtgggg atttagtcag aatgagttca aactgtagcg taagttacaa ttttacacca 1680
gctgatcagc aagttgtaat acgtctacgt tatgcttgtc aagggacagc ttcattaagg 1740
ataacgtttg gtaatggttc tagccaaata attccgcttg tttctacaac ttcatcaata 1800
aataatcttc aatatgaaaa ttttagtttt gcttctggtc caaatagcgt taacttttta 1860
tcagctggta cttcaataac tattcaaaat atcagtacaa attctaacgt agtgctagat 1920
agaattgaaa ttgtgccaga acaacctatt cctattattc caggggacta tcaaattgta 1980
acagctttaa ataatagtag tgtattcgat ttaaatagtg gaacccgagt tacattatgg 2040
tcgaataata gaggtgctca tcaaatttgg aatttcatgt atgatcagca aagaaatgca 2100
tatgtaatac gtaacgtaag taatccaagt ttagtcttaa cttgggattt tacaagtcct 2160
aatagtattg tatttgctgc ccctttttct ccaggaaggc aagagcaata ttggattgca 2220
gaaagttttc aaaatagcta tgtattcgaa aacctcagaa atacgaatat ggttttagat 2280
gtagccggag gatcaaccgc tattggtaca aatattatcg cattcccaag acataatgga 2340
aatgctcaaa gattcttcat cagaagacct 2370
<210> 10
<211> 2361
<212> DNA
<213> 苏云金芽孢杆菌
<400> 10
atgaattctt atcaaaataa gaatgaatat gaaatactgg atgcttcaga aaatactgta 60
aatgcgttaa acagatatcc tttcgcaaat aatccgtatt cttccatttt tagttcttgt 120
ccacgcagtg ggcctggtaa ttggattaat atactaggaa atgcagttag cgaagcagta 180
tctatttcgc aagatataat atctcttctt acacagcctt ctatctctgg gataatttca 240
atggcattta gtcttttaag tagaatgata ggtagtaatg gaaggtctat atcggagtta 300
tctatgtgtg acttactagc tattattgat ttgcgggtaa atcagagtgt tttggatgac 360
ggagttgctg attttaacgg ctcgttagtt atatacagaa actatttaga ggctttacaa 420
aggtggaaca ataatcccaa tcccgctaat gccgaagagg ttcgtactcg ttttagggaa 480
tctgatacaa tattcgatct cattttaaca caagggtctt taacgaatgg cggttcatta 540
gccagaaata atgctcaaat attattattg ccttcttttg caaatgctgc atactttcat 600
ttactgctat taagagatgc taatgtatat gggaataatt ggggtttatt tggggttaca 660
cctaatataa attatgaatc gaaattacta aaccttatta gattatatac taattattgc 720
acacattggt ataatcaagg actaaatgaa ctaagaaatc gaggttccaa tgctacagct 780
tggttggaat ttcatagatt tcgtagagat atgacattga tggtattgga tatagtatca 840
tcattttcaa gtcttgatat tactagatat ccaagagcaa cagattttca attgagtagg 900
ataatttata cagatccaat tggttttgta aatcgtagtg accctagcgc accaagaacc 960
tggtttagtt ttcacaatca agctaatttt tcagcgttag aaagtggaat acctagtcct 1020
tcattctcac aattcttaga tagtatgcgt atatctactg gcccgcttag tttacctgct 1080
tctcctaata tccatagagc acgggtatgg tatggtaatc aaaataactt taatggatct 1140
agtagccaaa cttttgggga aataacaaat gataatcaaa ccatttcggg tttaaatatt 1200
ttcagaatag attcacaggc tgttaatcta aataatacta cgtttggagt tagtagagct 1260
gaattttatc atgatgctag tcaaggctct caaagatcca tatatcaagg atttgttgat 1320
acaggtgggg ctagtaccgc tgtagcccag aatattcaaa catttttccc gggagaaaat 1380
tcgagtatac caactccaca agattatact catatattaa gtaggtcaac aaatttaaca 1440
ggaggacttc gacaagtagc atctggacgt cgttcttctt tagtattaca cggttggaca 1500
cataaaagtc tgagtcgtca aaatagagtt gaaccaaata gaattactca agtgccggct 1560
gttaaagcaa gttctccttc gaattgtact gtaattgcag gacctggatt tacaggtggg 1620
gatttagtca gaatgagttc aaactgtagc gtaagttaca attttacacc agctgatcag 1680
caagttgtaa tacgtctacg ttatgcttgt caagggacag cttcattaag gataacgttt 1740
ggtaatggtt ctagccaaat aattccgctt gtttctacaa cttcatcaat aaataatctt 1800
caatatgaaa attttagttt tgcttctggt ccaaatagcg ttaacttttt atcagctggt 1860
acttcaataa ctattcaaaa tatcagtaca aattctaacg tagtgctaga tagaattgaa 1920
attgtgccag aacaacctat tcctattatt ccaggggact atcaaattgt aacagcttta 1980
aataatagta gtgtattcga tttaaatagt ggaacccgag ttacattatg gtcgaataat 2040
agaggtgctc atcaaatttg gaatttcatg tatgatcagc aaagaaatgc atatgtaata 2100
cgtaacgtaa gtaatccaag tttagtctta acttgggatt ttacaagtcc taatagtatt 2160
gtatttgctg cccctttttc tccaggaagg caagagcaat attggattgc agaaagtttt 2220
caaaatagct atgtattcga aaacctcaga aatacgaata tggttttaga tgtagccgga 2280
ggatcaaccg ctattggtac aaatattatc gcattcccaa gacataatgg aaatgctcaa 2340
agattcttca tcagaagacc t 2361
<210> 11
<211> 3516
<212> DNA
<213> 苏云金芽孢杆菌
<400> 11
atggaggtaa ataatcaaaa tcaatgcgtg ccctataatt gtttgaataa tcctgaaatc 60
gaaatattag gaggagaaag aatatcagtt ggtaataccc caatcgatat ttctctgtcg 120
cttacgcagt ttcttttgag tgaatttgtc ccaggtgcgg ggtttgtatt agggttaatt 180
gatttaatat ggggattttt aggcccctct caatgggatg catttctttt gcaaattgaa 240
cagttaatta gccaaagaat agaggaattc gcaaggaacc aagcaatttc tagattagaa 300
gggctaagca atctttatcg catttacgca gaagctttta gagcgtggga agctgatcct 360
actaatctag cattaagaga agagatgcgt acgcaattta atgacatgaa cagtgctctt 420
gtaacagcta ttcctctttt ttcagttcaa aattatcaag tcccactttt atcagtatat 480
gttcaagctg caaatttaca tttatcggtt ttgagagatg tttcagtgtt tgggcaacgt 540
tggggatttg atgtagcaac aatcaatagt cgttataatg atttaactag gcttattggc 600
gaatatacgg attatgctgt acgctggtac aatacgggat tagatcgttt gcgaggttct 660
aatttccaag attggataag atacaatcgt tttagaagag aattaacact gactgtatta 720
gatatcgttt ctgtttttca aaactacgat tctagattat atccaattca aacatcatct 780
caattaacac gagaaattta ttcggattta cttttagcta atccatctgg agttggaagt 840
ttctctaatg tagatttcga tagtattcta attagacaac ctcatttaat agattttatg 900
agagtactta cgatttatac cgatcgacat aacgcaagta gacacaatat atattgggct 960
ggacatcaag tgactgccgt tgatactgct aatcgtacga ttgtgtatcc tgtaaatggt 1020
agtgcagcaa atttagaacc cccaagaact ttacgatttg aaagtccagt tgtagaaatt 1080
cgttcaaatc ctgtatggga tagaggatca actggaattg caggcagcta tgaatttttt 1140
ggggtgacaa gtgctttgtt tattacaatt ttgggatttg gttacactta tcgaagcgga 1200
tccaatacag aagttactgc attaccagac catcaagtga gtcatattgg ttattttaga 1260
cgttttacta caacgggtgc caccgctaga caaacgctaa caagtgcacc gatagtttcc 1320
tggacgcata gtagcgctga gccaccaaat agaatttatc agaatagaat tacccaaatc 1380
cctgctgtta aaggtaactt tctttttaat ggagctgtaa tctcaggacc aggatttact 1440
ggaggggact tagttagatt gaataggaat aatgataaca ttcaaaatag ggggtatatc 1500
gaagttccaa tccaattcgc gtcgacatct accagatatc gggttcgtgt acgttatgct 1560
tctacaaacg cgatcgaagt caatattaat tggggaaatg gatcaatttt tacgggcaca 1620
gcaccagcta cagctacatc attagataat ctacaatcaa acgattttgg ctattttgaa 1680
agtaccactg cttttgcacc ttcattaggt aatatagtag gtgttaggaa ttttagtgca 1740
aatgcagatg tgataataga cagatttgaa tttattccag ttactgcaac acttgaagca 1800
gaatatgacc tagaaagagc ggagaaggcg gtgaatgccc tgtttacttc cacaacccaa 1860
ttaggactaa aaacagatgt gacggattat catattgatc aggtatccaa tctagtagaa 1920
tgcctatcgg atgaattctg cctaaatgaa aagagagaat tatccgagaa agtcaaacat 1980
gcgaagcgac ttagtgataa aaggaattta ctccaagatc caaatttcac atccattaat 2040
gggcaactag accgtgggtg gagaggaagc acggatatta ctatccaagg aggcaatgac 2100
gtattcaaag agaattacgt gacactaccg ggcacctttg acgagtgcta tccaacgtat 2160
ttgtatcaaa aaatagatga gtcacaatta aaatcttata ctcgctatca attaagaggc 2220
tatatcgaag atagtcaaga tttagagatt tatttgattc gttacaatgc gaaacatgaa 2280
acattaagtg tgccaggtac tgagtcccca tggccatctt caggagtata tccaattgga 2340
aagtgcggag aaccgaatcg atgtgcacca cgaatcgaat ggaatcccga tctaggctgt 2400
tcctgcagat atggagagaa atgcgttcat cattcgcatc atttctcctt ggatattgat 2460
gttggatgta cagatttgaa tgaggatcta ggcgtatggg tgatatttaa gattaagacc 2520
caagatggcc atgcaaaact aggaaaccta gaattcatcg aagagaaacc attattagga 2580
gaagcgctgt cccgtgtgaa gagggccgag aaaaaatgga aagacaaatg tgaaaaactg 2640
caattggaaa cacaacgagt atatacagag gcaaaagaat ctgtggatgc tttattcata 2700
gattctcaat atgatagatt acaagcagat acaaacattg gtatgattca tgcggcagat 2760
aaacaggttc atcgaatccg agaagcgtat ctcccggaat tacacgcgat tccaggtgta 2820
aatgcggaaa ttttcgaaga attagaaaat ttccgcattt acactgcatt ctctctatat 2880
gatgcaagaa atgtcataaa aaatggcgat ttcaataatg gtttatcgtg ttggaacgta 2940
aaagggcatg tagatgtaca acagaaccat catcgctcgg tccttgttct ctcagaatgg 3000
gaagcagaag tgtcacaaaa ggtacgcgta tgtccagatc gaggctatat ccttcgtgtt 3060
acagcgtata aagagggata tggagaggga tgcgtaacga ttcatgaatt cgaagataat 3120
acggatgtac tgaagtttag aaactgtgta gaagaggaag tatatccaaa caacacggta 3180
acgtgtaatg attatactac gaatcaaagt gcagaaggat gtacggatgc atgtaattcc 3240
tataatcgtg gatatgagga tggatatgga aacaatcctt cagcaccagt taattacaca 3300
ccgacgtacg aagaaagaat gtatacagat acagatacac agggatataa tcattgtgta 3360
tctgacagag gatataggaa tcatacacca ttaccagcgg gctatgtaac gctagaatta 3420
gaatttttcc cagaaacaga gcaagtatgg atagagattg gggaaacaga aggaacattc 3480
atcgtggaca gtgtagaatt attccttatg gaggaa 3516
<210> 12
<211> 1782
<212> DNA
<213> 苏云金芽孢杆菌
<400> 12
atggaggtaa ataatcaaaa tcaatgcgtg ccctataatt gtttgaataa tcctgaaatc 60
gaaatattag gaggagaaag aatatcagtt ggtaataccc caatcgatat ttctctgtcg 120
cttacgcagt ttcttttgag tgaatttgtc ccaggtgcgg ggtttgtatt agggttaatt 180
gatttaatat ggggattttt aggcccctct caatgggatg catttctttt gcaaattgaa 240
cagttaatta gccaaagaat agaggaattc gcaaggaacc aagcaatttc tagattagaa 300
gggctaagca atctttatcg catttacgca gaagctttta gagcgtggga agctgatcct 360
actaatctag cattaagaga agagatgcgt acgcaattta atgacatgaa cagtgctctt 420
gtaacagcta ttcctctttt ttcagttcaa aattatcaag tcccactttt atcagtatat 480
gttcaagctg caaatttaca tttatcggtt ttgagagatg tttcagtgtt tgggcaacgt 540
tggggatttg atgtagcaac aatcaatagt cgttataatg atttaactag gcttattggc 600
gaatatacgg attatgctgt acgctggtac aatacgggat tagatcgttt gcgaggttct 660
aatttccaag attggataag atacaatcgt tttagaagag aattaacact gactgtatta 720
gatatcgttt ctgtttttca aaactacgat tctagattat atccaattca aacatcatct 780
caattaacac gagaaattta ttcggattta cttttagcta atccatctgg agttggaagt 840
ttctctaatg tagatttcga tagtattcta attagacaac ctcatttaat agattttatg 900
agagtactta cgatttatac cgatcgacat aacgcaagta gacacaatat atattgggct 960
ggacatcaag tgactgccgt tgatactgct aatcgtacga ttgtgtatcc tgtaaatggt 1020
agtgcagcaa atttagaacc cccaagaact ttacgatttg aaagtccagt tgtagaaatt 1080
cgttcaaatc ctgtatggga tagaggatca actggaattg caggcagcta tgaatttttt 1140
ggggtgacaa gtgctttgtt tattacaatt ttgggatttg gttacactta tcgaagcgga 1200
tccaatacag aagttactgc attaccagac catcaagtga gtcatattgg ttattttaga 1260
cgttttacta caacgggtgc caccgctaga caaacgctaa caagtgcacc gatagtttcc 1320
tggacgcata gtagcgctga gccaccaaat agaatttatc agaatagaat tacccaaatc 1380
cctgctgtta aaggtaactt tctttttaat ggagctgtaa tctcaggacc aggatttact 1440
ggaggggact tagttagatt gaataggaat aatgataaca ttcaaaatag ggggtatatc 1500
gaagttccaa tccaattcgc gtcgacatct accagatatc gggttcgtgt acgttatgct 1560
tctacaaacg cgatcgaagt caatattaat tggggaaatg gatcaatttt tacgggcaca 1620
gcaccagcta cagctacatc attagataat ctacaatcaa acgattttgg ctattttgaa 1680
agtaccactg cttttgcacc ttcattaggt aatatagtag gtgttaggaa ttttagtgca 1740
aatgcagatg tgataataga cagatttgaa tttattccag tt 1782
<210> 13
<211> 999
<212> DNA
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 13
atgaaagtgt ataaaaaaat aacgaaaatg gcaccaatta tggcattaag tacagctgta 60
ttattgtctc caggatctac ttttgcagct gaaaaagcag ttgttacaaa atcaaatgta 120
tcttctctaa caactaatac agtaatgcaa tcaggaagta tcattcaagg atatctaatt 180
aaaaatggtg tcaaaacccc cgtatataac agtgaggtac aaactcggtc tacagcggta 240
aatgaagcac cctatccaga actttcaagt aatccaaatg atccagttcc ttcaaaagga 300
tccatcacaa gtgaaagtgg aaatgtagga tcggtactat atttttctaa atttaattcg 360
caaaaattac aaaatactgc ggaaccggtt tattggaaaa atgtatattt agaaaaaact 420
ccggatggga atattatttt tggaacgtat gatccgacaa ctttaaagcg gactcctaat 480
ctggttaata ttatgatgac tccttcaaag gtacaatatt accaatcctt ctttactgat 540
acaaaaataa aacgagaaac tgcgtatgaa aaaataggtg gaggaactcc acaacccaaa 600
aatacttcgt atacattttc aagtgctgtt acgtctggat tatctacatc agatgcaatc 660
ggtggttctc tgacattagg atataaatat agtgttaaag aaggtggtgg tgtacttcct 720
gttgaagcga cacaagaatt tagtttacaa ttaacggcaa gttataacca tacaatcact 780
gtttccagtc aaacaactaa tacacaaact tatagtgtag cacacgctgg agattcgtat 840
aaaaatgata aatatgtagc ggctatgtat cagttaaaat ctcattatac agttattcca 900
ggacctgcac taacacaatc gggaagtatt ttagctcaag aggcattcca atatgatgat 960
tcatctctgt atttagccgt gactcctggt gctggaatt 999
<210> 14
<211> 915
<212> DNA
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 14
atggaaaaag cagttgttac aaaatcaaat gtatcttctc taacaactaa tacagtaatg 60
caatcaggaa gtatcattca aggatatcta attaaaaatg gtgtcaaaac ccccgtatat 120
aacagtgagg tacaaactcg gtctacagcg gtaaatgaag caccctatcc agaactttca 180
agtaatccaa atgatccagt tccttcaaaa ggatccatca caagtgaaag tggaaatgta 240
ggatcggtac tatatttttc taaatttaat tcgcaaaaat tacaaaatac tgcggaaccg 300
gtttattgga aaaatgtata tttagaaaaa actccggatg ggaatattat ttttggaacg 360
tatgatccga caactttaaa gcggactcct aatctggtta atattatgat gactccttca 420
aaggtacaat attaccaatc cttctttact gatacaaaaa taaaacgaga aactgcgtat 480
gaaaaaatag gtggaggaac tccacaaccc aaaaatactt cgtatacatt ttcaagtgct 540
gttacgtctg gattatctac atcagatgca atcggtggtt ctctgacatt aggatataaa 600
tatagtgtta aagaaggtgg tggtgtactt cctgttgaag cgacacaaga atttagttta 660
caattaacgg caagttataa ccatacaatc actgtttcca gtcaaacaac taatacacaa 720
acttatagtg tagcacacgc tggagattcg tataaaaatg ataaatatgt agcggctatg 780
tatcagttaa aatctcatta tacagttatt ccaggacctg cactaacaca atcgggaagt 840
attttagctc aagaggcatt ccaatatgat gattcatctc tgtatttagc cgtgactcct 900
ggtgctggaa tttag 915
<210> 15
<211> 1155
<212> DNA
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 15
atggcaatta taaatcaatc atcactaaat tcaagaatac atgatttacg tgaagattca 60
agaacagctc ttgaaaaagt ttatactagt aataatcctt ggggtttcgt aagtatacac 120
tctgaccgac ttgaaaatta tcaactaact aatgtaaatg ttagtcctag gaatcaagat 180
tttcaaacga ttcctagatt gcaacactct gctacacaaa taattgaaaa taacacaagt 240
gtaacacaat ctcaaaccat ttcttttaat gaaagaacaa cagacacttt tacaacatcg 300
gttactacgg gatttaaaac tggaactagt gtgaaatcta cgacaaaatt caaaatatct 360
gttggatttt tattagcagg cgaattagaa caatcagtgg aagtttctgt gaattttgag 420
tataattata gttcaacaac tacagagacg catagcgttg aaagaggatg gacaatttca 480
cagcctataa ttgctccccc acgaacaagg gtagaagcta ctcttctaat ttatgctgga 540
tctgttgatg taccaattga tttaaatgct accattgttg gtgatccaat tccatggcca 600
tcgtgggggc cggcagtata ttctggatct tttcttgcta atgatggtcg gatatggtcg 660
gctcctatac taccagagca actatcactg gcatcttcag cgtatacaac tgttggaagg 720
acagcaaatt ttagcggttt agcgactacc aacgtttcct caggcctgta ttctattgtt 780
cgtattgatg aaagtccttt accaggattt acaggagaaa caaggcgtta ttatttaccg 840
ccttcattag cgactacaaa tcaaatactt tcgacaaatg cgttaggaaa taatgtgcca 900
attattaatc cagttcctaa tggacattgc aaaaaagatc attctccaat tattattcat 960
aaaaatagag aggtgaagtg cgaacacaat tatgatgaag tgtatcctcg tcatgatcaa 1020
gtagagaagt gcgaacacaa ttatgatgaa gtgtatcctc gtcatgatca agtagagaag 1080
tgcgaacacg attatgatga agtgtatcct cgtcatgatc aagtagagaa gtacgaacac 1140
aattatgatg aagaa 1155
<210> 16
<211> 1002
<212> DNA
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 16
atggcaatta taaatcaatc atcactaaat tcaagaatac atgatttacg tgaagattca 60
agaacagctc ttgaaaaagt ttatactagt aataatcctt ggggtttcgt aagtatacac 120
tctgaccgac ttgaaaatta tcaactaact aatgtaaatg ttagtcctag gaatcaagat 180
tttcaaacga ttcctagatt gcaacactct gctacacaaa taattgaaaa taacacaagt 240
gtaacacaat ctcaaaccat ttcttttaat gaaagaacaa cagacacttt tacaacatcg 300
gttactacgg gatttaaaac tggaactagt gtgaaatcta cgacaaaatt caaaatatct 360
gttggatttt tattagcagg cgaattagaa caatcagtgg aagtttctgt gaattttgag 420
tataattata gttcaacaac tacagagacg catagcgttg aaagaggatg gacaatttca 480
cagcctataa ttgctccccc acgaacaagg gtagaagcta ctcttctaat ttatgctgga 540
tctgttgatg taccaattga tttaaatgct accattgttg gtgatccaat tccatggcca 600
tcgtgggggc cggcagtata ttctggatct tttcttgcta atgatggtcg gatatggtcg 660
gctcctatac taccagagca actatcactg gcatcttcag cgtatacaac tgttggaagg 720
acagcaaatt ttagcggttt agcgactacc aacgtttcct caggcctgta ttctattgtt 780
cgtattgatg aaagtccttt accaggattt acaggagaaa caaggcgtta ttatttaccg 840
ccttcattag cgactacaaa tcaaatactt tcgacaaatg cgttaggaaa taatgtgcca 900
attattaatc cagttcctaa tggacattgc aaaaaagatc attctccaat tattattcat 960
aaaaatagag aggtgaagtg cgaacacaat tatgatgaag aa 1002
<210> 17
<211> 3696
<212> DNA
<213> 苏云金芽孢杆菌
<400> 17
atgaataaaa ataatcaaaa tgaatatgaa attattgacg cttccaattg tggttgtgcg 60
tctgatgatg ttgcgagata tcctttagcc aacaatccgt attcatctgc tttaaattta 120
aattcttgtc aaaatagtag cattctcaat tggattaaca taataggaaa tgcagcaaaa 180
gaagcagtat ctattggatt aacaataaaa tctcttatca cagcaccttc tctcactgga 240
ttaatttcca tagcatataa tcttttgggg aaagtgctag gaggtagtag tggccaatcc 300
atatcagatt tgtctatatg tgacttatta tctattattg atttgcgggt aaatcagagt 360
gttttaaatg atgggattgc agattttaat ggttctttaa tcttatacag gaactatttg 420
gatgctctaa atagctggaa tgagaatcct aattctaatc gggctgaaga actccgtgcc 480
cgttttagaa tcgctgattc agaatttgat agaattttaa cacgggggtc tttaacgaat 540
ggtggttcgt tagctagaca agatgcccaa atattattat taccttcttt tgcaagtgct 600
gcatttttcc atttattact actaagggat gctgctagat atggaaatga ttgggatctt 660
tttggcgcta taccttttat aaattatcaa tccaaactag tagaacttat tgaactatat 720
actgattatt gcgtaaattg gtataatcaa ggtttcaacg aactaagaca acgaggcact 780
agtgctacag tttggttgga atttcataga tatcgtagag agatgacatt gacggtatta 840
gatatagtag catcattttc aagtcttgat attactaact acccaataga aacagatttt 900
cagttgagta ggattattta tacagatcca attggttttg tacatcgtag tagtcttagg 960
ggggagagtt ggtttagctt tattaataga gctaatttct cagagttaga aaatgcaata 1020
cctaacccta gaccgtcttg gtttttaaat aatatgatta tatctactgg ttcacttaca 1080
ttgcccgtta gtccaaatac tgatagagcg agagtatggt atgggagccg agatagaatt 1140
tcccctgcta attcacaagt aatttctgag ctgatttcgg ggcaacatac gaattctaca 1200
caaactattt tagggcgaaa tatatttaga atagattctc aagcatgtaa tttaaatgat 1260
accacatatg gagtaaacag ggctgtattt tatcatgatg ctagtgaagg ttctcaaaga 1320
tcagtgtacg aagggtttat tagaacaact ggaatagata atcctagagt tcagaatatt 1380
aatacttatt ttcctggaga aaattcaaat atcccaactc cagaagacta tactcattta 1440
ttaagtacaa cagtaaattt aacaggaggt cttagacaag tagcaaataa tcgtcgttca 1500
tctatagtaa tttatggttg gacacataaa agtctaactc gtaacaatac tattaatcca 1560
ggtattatta cacaaatccc aatggttaaa ttatccaatc tctcttcagg tactaatgtt 1620
gttagagggc caggatttac aggtggagat atccttcgta gaacgaatgc tggtaacttt 1680
ggagatgtac gagtcaatat tgctggatca ttatcccaaa gatatcgcgt aaggattcgt 1740
tatgcttcta ctacaaattt acaattccac acatcaatta acggaagagc tattaatcaa 1800
gcgaattttc cagcaactat gaatataggt gctagcttaa actatagaac ctttagaact 1860
gtaggattta caactccatt tactttttca gaagcatcaa gcatatttac attaagtact 1920
cattccttca gttcaggcaa tgcagtttat atagatcgaa ttgaatttgt cccggcagaa 1980
gtaacattcg aggcagaatc tgatctagaa agagcacaga aggcggtgaa tgcgctgttt 2040
acttcttcca atcaaatcgg cttaaaaaca gatgtgacgg actatcatat tgatcaagtt 2100
tccaatttag ttgcgtgttt atcggatgaa ttttgtctgg atgaaaagcg agagttgtcc 2160
gagaaagtca aacatgcgaa gcgactcagt gatgagcgaa atttacttca agatccaaac 2220
ttcagaggca tcaatagaca actagaccgt ggttggagag gaagtacgga tattaccatc 2280
caaggtggag atgacgtatt caaagagaat tacgtcacac tgccgggtac ctttgatgag 2340
tgctatccaa catatttata tcaaaaaata gatgagtcga aattaaaagc ctatacccgc 2400
tatgaattaa gagggtatat tgaagatagt caagacttag aagtctattt gatccgttac 2460
aatgcaaaac acgaaacgtt aaatgtgcca ggtacgggtt ccttatggcc acttgcagcc 2520
gaaagttcaa tcgggaggtg cggcgaaccg aatcgatgcg cgccacatat tgaatggaat 2580
cctgacctag attgttcgtg tagggatgga gaaaaatgtg cacatcattc tcatcatttc 2640
tccttggata ttgatgttgg atgtacagac ttaaatgagg atttaggtgt atgggtgata 2700
ttcaagatta agacgcaaga tggccacgca agacttggaa atctagagtt tctcgaagag 2760
aaaccattat taggagaagc gctagctcgt gtgaagagag cggagaaaaa atggagagac 2820
aaacgcgaca aattggaatt ggaaacaaat attgtttata aagaggcaaa agaatctgta 2880
gatgctttat tcgtagattc tcaatataat agattacaaa cggatacgaa cattgcgatg 2940
attcatgcgg cagataaacg cgttcatcga atccgagaag cgtatctgcc agagttgtct 3000
gtgattccgg gtgtcaatgc ggctattttc gaagaattag aaggtcttat tttcactgca 3060
ttctccctat atgatgcgag aaatgtcatt aaaaacggag atttcaatca tggtttatca 3120
tgctggaacg tgaaagggca tgtagatgta gaagaacaaa ataaccaccg ttcggtcctt 3180
gttgttccgg aatgggaagc agaagtgtca caagaagtcc gcgtatgtcc aggacgtggc 3240
tatatcctgc gtgttacagc gtacaaagag ggctacggag aaggatgcgt aacgatccat 3300
gaaattgaag atcatacaga cgaactgaaa tttagaaact gtgaagaaga ggaagtgtat 3360
ccgaataaca cggtaacgtg taatgattat ccagcaaatc aagaagaata caagggtgcg 3420
tacccttctc gtaatggtgg atatgaggat acatatgaca cttcagcatc tgttcattac 3480
aacacaccaa cgtacgaaga agaaatagga acagatctac agagatataa tcagtgtgaa 3540
aataacagag gatatggaaa ttacacacca ctaccagcag gttatgtaac aaaagaatta 3600
gagtacttcc cagaaacaga taaagtatgg atagagattg gcgaaacgga aggaacattc 3660
atcgtagaca gtgtggaatt actcctcatg gaggaa 3696
<210> 18
<211> 1980
<212> DNA
<213> 苏云金芽孢杆菌
<400> 18
atgaataaaa ataatcaaaa tgaatatgaa attattgacg cttccaattg tggttgtgcg 60
tctgatgatg ttgcgagata tcctttagcc aacaatccgt attcatctgc tttaaattta 120
aattcttgtc aaaatagtag cattctcaat tggattaaca taataggaaa tgcagcaaaa 180
gaagcagtat ctattggatt aacaataaaa tctcttatca cagcaccttc tctcactgga 240
ttaatttcca tagcatataa tcttttgggg aaagtgctag gaggtagtag tggccaatcc 300
atatcagatt tgtctatatg tgacttatta tctattattg atttgcgggt aaatcagagt 360
gttttaaatg atgggattgc agattttaat ggttctttaa tcttatacag gaactatttg 420
gatgctctaa atagctggaa tgagaatcct aattctaatc gggctgaaga actccgtgcc 480
cgttttagaa tcgctgattc agaatttgat agaattttaa cacgggggtc tttaacgaat 540
ggtggttcgt tagctagaca agatgcccaa atattattat taccttcttt tgcaagtgct 600
gcatttttcc atttattact actaagggat gctgctagat atggaaatga ttgggatctt 660
tttggcgcta taccttttat aaattatcaa tccaaactag tagaacttat tgaactatat 720
actgattatt gcgtaaattg gtataatcaa ggtttcaacg aactaagaca acgaggcact 780
agtgctacag tttggttgga atttcataga tatcgtagag agatgacatt gacggtatta 840
gatatagtag catcattttc aagtcttgat attactaact acccaataga aacagatttt 900
cagttgagta ggattattta tacagatcca attggttttg tacatcgtag tagtcttagg 960
ggggagagtt ggtttagctt tattaataga gctaatttct cagagttaga aaatgcaata 1020
cctaacccta gaccgtcttg gtttttaaat aatatgatta tatctactgg ttcacttaca 1080
ttgcccgtta gtccaaatac tgatagagcg agagtatggt atgggagccg agatagaatt 1140
tcccctgcta attcacaagt aatttctgag ctgatttcgg ggcaacatac gaattctaca 1200
caaactattt tagggcgaaa tatatttaga atagattctc aagcatgtaa tttaaatgat 1260
accacatatg gagtaaacag ggctgtattt tatcatgatg ctagtgaagg ttctcaaaga 1320
tcagtgtacg aagggtttat tagaacaact ggaatagata atcctagagt tcagaatatt 1380
aatacttatt ttcctggaga aaattcaaat atcccaactc cagaagacta tactcattta 1440
ttaagtacaa cagtaaattt aacaggaggt cttagacaag tagcaaataa tcgtcgttca 1500
tctatagtaa tttatggttg gacacataaa agtctaactc gtaacaatac tattaatcca 1560
ggtattatta cacaaatccc aatggttaaa ttatccaatc tctcttcagg tactaatgtt 1620
gttagagggc caggatttac aggtggagat atccttcgta gaacgaatgc tggtaacttt 1680
ggagatgtac gagtcaatat tgctggatca ttatcccaaa gatatcgcgt aaggattcgt 1740
tatgcttcta ctacaaattt acaattccac acatcaatta acggaagagc tattaatcaa 1800
gcgaattttc cagcaactat gaatataggt gctagcttaa actatagaac ctttagaact 1860
gtaggattta caactccatt tactttttca gaagcatcaa gcatatttac attaagtact 1920
cattccttca gttcaggcaa tgcagtttat atagatcgaa ttgaatttgt cccggcataa 1980
<210> 19
<211> 4008
<212> DNA
<213> 苏云金芽孢杆菌
<400> 19
aatataacct atatttatat atagacaatt aatatacttt attaaatata tataggctat 60
atatttatat tcataaaatt agtagaattt tatgaatatt ttcataaact gaaccatatg 120
atttaaactg aggtaaagat aaatggccct aaattatgaa ggtatatgtg ctaaagtcca 180
aaaaacggga ggtaattcat caaaaaatcg tactatacaa tttgcttagg taatgctgtg 240
ttgaaactac tcagtggaga aaaattaaat agttggtaat gtaagcacaa cgtaaaagga 300
ggagttatat tgacttcaaa taggaaaaat gagaatgaaa ttataaatgc tttatcgatt 360
ccagctgtat cgaatcattc cgcacaaatg gatctatcac cagatgctcg tattgaggat 420
agcttgtgta tagccgaggg gaacaatatc gatccatttg ttagcgcatc aacagtccaa 480
acgggtatta acatagctgg tagaatacta ggcgtattgg gcgtaccgtt tgctggacaa 540
ctagctagtt tttatagttt tcttgttggt gaattatggc ctagcggcag agatccatgg 600
gaaattttta tggaacatgt cgaacaactt gtaagacaac aaataacgga cagtgttagg 660
gataccgcta ttgctcgttt agaaggtcta ggaagagggt atagatctta ccagcaggct 720
cttgaaactt ggttagataa ccgaaatgat gcaagatcaa gaagcattat tcttgagaga 780
tatattgctt tagaacttga cattactact gctataccgc ttttcagcat acgaaatcaa 840
gaggttccat tattaatggt atatgctcaa gctgcaaatt tacacctatt attattgaga 900
gacgcatccc tttttggtag tgaatggggg atgtcatctg ccgatgttaa ccaatattac 960
caagaacaaa tcagatatac agaggaatat tctaaccatt gcgtgcaatg gtataatacg 1020
ggtctaaata acctaagagg gacaaatgct gaaagctggg tacggtataa tcaattccgc 1080
agagacctaa cattaggagt attagatcta gtggccctat tcccaagcta tgatactcgc 1140
acttatccaa taaatacgag tgctcagtta acaagagaag tttatacaga cgcaattgga 1200
gcaacagggg taaatatggc aaatatgaat tggtacaata ataatgcacc ttcgttctcc 1260
gctatagagg ctgcggttat cagaagcccg catctacttg attttctaga acaacttaca 1320
atttttagcg cttcatcacg atggagtaat actaggcata tgacttactg gcgggggcac 1380
acgattcaat ctcggccaat aggaggcgga ttaaacacct caacgtatgg gtctaccaat 1440
acttctatta atcctgtaac attacggttc acgtctcgag acgtctatag gacagaatca 1500
tgggcaggag tgcttctatg gggaatttac cttgaaccta ttcatggtgt ccctactgtt 1560
aggtttaatt ttacgaaccc tcagaatatt tatgatagag gtactgctaa ctatagtcaa 1620
ccgtacgagt cacctgggct tcaattaaaa gattcagaaa cggaattacc gccagaaaca 1680
acagaacgac caaattatga atcttacagt cataggttat ctcatatagg tataatttta 1740
caatccaggg tgaatgtacc ggtatattct tggacgcatc gtagtgcaga tcgtacgaat 1800
acgattggac caaatagaat cacccaaatc ccaatggtaa aagcatccga acttcctcaa 1860
ggtaccactg ttgttagagg accaggattt actggtgggg atattcttcg aagaacgaat 1920
actggtggat ttggaccgat aagagtaact gttaacggac cattaacaca aagatatcgt 1980
ataggattcc gctatgcttc aactgtagat tttgatttct ttgtatcacg tggaggtact 2040
actgtaaata attttagatt cctacgtaca atgaacagtg gagacgaact aaaatacgga 2100
aattttgtga gacgtgcttt tactacacct tttactttta cacaaattca agatataatt 2160
cgaacgtcta ttcaaggcct tagtggaaat ggggaagtgt atatagataa aattgaaatt 2220
attccagtta ctgcaacctt cgaagcagaa tatgatttag aaagagcgca agaggcggtg 2280
aatgctctgt ttactaatac gaatccaaga agattgaaaa cagatgtgac agattatcat 2340
attgatcaag tatccaattt agtggcgtgt ttatcggatg aattctgctt ggatgaaaag 2400
agagaattac ttgagaaagt gaaatatgcg aaacgactca gtgatgaaag aaacttactc 2460
caagatccaa acttcacatc catcaataag caaccagact tcatatctac taatgagcaa 2520
tcgaatttca catctatcca tgaacaatct gaacatggat ggtggggaag tgagaacatt 2580
accatccagg aaggaaatga cgtatttaaa gagaattacg tcacactacc gggtactttt 2640
aatgagtgtt atccgacgta tttatatcaa aaaatagggg agtcggaatt aaaagcttat 2700
actcgctacc aattaagagg ttatattgaa gatagtcaag atttagagat atatttgatt 2760
cgttataatg cgaaacctga aacattggat gttccaggta ccgagtccct atggccgctt 2820
tcagttgaaa gcccaatcgg aaggtgcgga gaaccgaatc gatgcgcacc acattttgaa 2880
tggaatcctg atctagattg ttcctgcaga gatggagaaa aatgtgcgca tcattcccat 2940
catttctctt tggatattga tgttggatgc acagacttgc atgagaatct aggcgtgtgg 3000
gtggtattca agattaagac gcaggaaggt catgcaagac tagggaatct ggaatttatt 3060
gaagagaaac cattattagg agaagcactg tctcgtgtga agagggcaga gaaaaaatgg 3120
agagacaaac gtgaaaaact acaattggaa acaaaacgag tatatacaga ggcaaaagaa 3180
gctgtggatg ctttattcgt agattctcaa tatgatagat tacaagcgga tacaaacatc 3240
ggcatgattc atgcggcaga taaacttgtt catcgaattc gagaggcgta tctttcagaa 3300
ttacctgtta tcccaggtgt aaatgcggaa atttttgaag aattagaagg tcacattatc 3360
actgcaatct ccttatacga tgcgagaaat gtcgttaaaa atggtgattt taataatgga 3420
ttaacatgtt ggaatgtaaa agggcatgta gatgtacaac agagccatca tcgttctgac 3480
cttgttatcc cagaatggga agcagaagtg tcacaagcag ttcgcgtctg tccggggtgt 3540
ggctatatcc ttcgtgtcac agcgtacaaa gagggatatg gagagggctg cgtaacgatc 3600
catgaaatcg agaacaatac agacgaacta aaatttaaaa accgtgaaga agaggaagtg 3660
tatccaacgg atacaggaac gtgtaatgat tatactgcac accaaggtac agctggatgc 3720
gcagatgcat gtaattcccg taatgctgga tatgaggatg catatgaagt tgatactaca 3780
gcatctgtta attacaaacc gacttatgaa gaagaaacgt atacagatgt aagaagagat 3840
aatcattgtg aatatgacag agggtatgtc aattatccac cagtaccagc tggttatgtg 3900
acaaaagaat tagaatactt cccagaaaca gatacagtat ggattgagat tggagaaacg 3960
gaaggaaagt ttattgtaga tagcgtggaa ttactcctca tggaagaa 4008
<210> 20
<211> 1920
<212> DNA
<213> 苏云金芽孢杆菌
<400> 20
atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60
tcgaatcatt ccgcacaaat ggatctatca ccagatgctc gtattgagga tagcttgtgt 120
atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180
aacatagctg gtagaatact aggcgtattg ggcgtaccgt ttgctggaca actagctagt 240
ttttatagtt ttcttgttgg tgaattatgg cctagcggca gagatccatg ggaaattttt 300
atggaacatg tcgaacaact tgtaagacaa caaataacgg acagtgttag ggataccgct 360
attgctcgtt tagaaggtct aggaagaggg tatagatctt accagcaggc tcttgaaact 420
tggttagata accgaaatga tgcaagatca agaagcatta ttcttgagag atatattgct 480
ttagaacttg acattactac tgctataccg cttttcagca tacgaaatca agaggttcca 540
ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agacgcatcc 600
ctttttggta gtgaatgggg gatgtcatct gccgatgtta accaatatta ccaagaacaa 660
atcagatata cagaggaata ttctaaccat tgcgtgcaat ggtataatac gggtctaaat 720
aacctaagag ggacaaatgc tgaaagctgg gtacggtata atcaattccg cagagaccta 780
acattaggag tattagatct agtggcccta ttcccaagct atgatactcg cacttatcca 840
ataaatacga gtgctcagtt aacaagagaa gtttatacag acgcaattgg agcaacaggg 900
gtaaatatgg caaatatgaa ttggtacaat aataatgcac cttcgttctc cgctatagag 960
gctgcggtta tcagaagccc gcatctactt gattttctag aacaacttac aatttttagc 1020
gcttcatcac gatggagtaa tactaggcat atgacttact ggcgggggca cacgattcaa 1080
tctcggccaa taggaggcgg attaaacacc tcaacgtatg ggtctaccaa tacttctatt 1140
aatcctgtaa cattacggtt cacgtctcga gacgtctata ggacagaatc atgggcagga 1200
gtgcttctat ggggaattta ccttgaacct attcatggtg tccctactgt taggtttaat 1260
tttacgaacc ctcagaatat ttatgataga ggtactgcta actatagtca accgtacgag 1320
tcacctgggc ttcaattaaa agattcagaa acggaattac cgccagaaac aacagaacga 1380
ccaaattatg aatcttacag tcataggtta tctcatatag gtataatttt acaatccagg 1440
gtgaatgtac cggtatattc ttggacgcat cgtagtgcag atcgtacgaa tacgattgga 1500
ccaaatagaa tcacccaaat cccaatggta aaagcatccg aacttcctca aggtaccact 1560
gttgttagag gaccaggatt tactggtggg gatattcttc gaagaacgaa tactggtgga 1620
tttggaccga taagagtaac tgttaacgga ccattaacac aaagatatcg tataggattc 1680
cgctatgctt caactgtaga ttttgatttc tttgtatcac gtggaggtac tactgtaaat 1740
aattttagat tcctacgtac aatgaacagt ggagacgaac taaaatacgg aaattttgtg 1800
agacgtgctt ttactacacc ttttactttt acacaaattc aagatataat tcgaacgtct 1860
attcaaggcc ttagtggaaa tggggaagtg tatatagata aaattgaaat tattccagtt 1920
<210> 21
<211> 794
<212> PRT
<213> 苏云金芽孢杆菌
<400> 21
Met Thr Cys Gln Leu Gln Ala Gln Pro Leu Ile Pro Tyr Asn Val Leu
1 5 10 15
Ala Gly Val Pro Thr Ser Asn Thr Gly Ser Pro Ile Gly Asn Ala Gly
20 25 30
Asn Gln Phe Asp Gln Phe Glu Gln Thr Val Lys Glu Leu Lys Glu Ala
35 40 45
Trp Glu Ala Phe Gln Lys Asn Gly Ser Phe Ser Leu Ala Ala Leu Glu
50 55 60
Lys Gly Phe Asp Ala Ala Ile Gly Gly Gly Ser Phe Asp Tyr Leu Gly
65 70 75 80
Leu Val Gln Ala Gly Leu Gly Leu Val Gly Thr Leu Gly Ala Ala Ile
85 90 95
Pro Gly Val Ser Val Ala Val Pro Leu Ile Ser Met Leu Val Gly Val
100 105 110
Phe Trp Pro Lys Gly Thr Asn Asn Gln Glu Asn Leu Ile Thr Val Ile
115 120 125
Asp Lys Glu Val Gln Arg Ile Leu Asp Glu Lys Leu Ser Asp Gln Leu
130 135 140
Ile Lys Lys Leu Asn Ala Asp Leu Asn Ala Phe Thr Asp Leu Val Thr
145 150 155 160
Arg Leu Glu Glu Val Ile Ile Asp Ala Thr Phe Glu Asn His Lys Pro
165 170 175
Val Leu Gln Val Ser Lys Ser Asn Tyr Met Lys Val Asp Ser Ala Tyr
180 185 190
Phe Ser Thr Gly Gly Ile Leu Thr Leu Gly Met Ser Asp Phe Leu Thr
195 200 205
Asp Thr Tyr Ser Lys Leu Thr Phe Pro Leu Tyr Val Leu Gly Ala Thr
210 215 220
Met Lys Leu Ser Ala Tyr His Ser Tyr Ile Gln Phe Gly Asn Thr Trp
225 230 235 240
Leu Asn Lys Val Tyr Asp Leu Ser Ser Asp Glu Gly Lys Thr Met Ser
245 250 255
Gln Ala Leu Ala Arg Ala Lys Gln His Met Arg Gln Asp Ile Ala Phe
260 265 270
Tyr Thr Ser Gln Ala Leu Asn Met Phe Thr Gly Asn Leu Pro Ser Leu
275 280 285
Ser Ser Asn Lys Tyr Ala Ile Asn Asp Tyr Asn Val Tyr Thr Arg Ala
290 295 300
Met Val Leu Asn Gly Leu Asp Ile Val Ala Thr Trp Pro Thr Leu Tyr
305 310 315 320
Pro Asp Asp Tyr Ser Ser Gln Ile Lys Leu Glu Lys Thr Arg Val Ile
325 330 335
Phe Ser Asp Met Val Gly Gln Ser Glu Ser Arg Asp Gly Ser Val Thr
340 345 350
Ile Lys Asn Ile Phe Asp Asn Thr Asp Ser His Gln His Gly Ser Ile
355 360 365
Gly Leu Asn Ser Ile Ser Tyr Phe Pro Asp Glu Leu Gln Lys Ala Gln
370 375 380
Leu Arg Met Tyr Asp Tyr Asn His Lys Pro Tyr Cys Thr Asp Cys Phe
385 390 395 400
Cys Trp Pro Tyr Gly Val Ile Leu Asn Tyr Asn Lys Asn Thr Phe Arg
405 410 415
Tyr Gly Asp Asn Asp Pro Gly Leu Ser Gly Asp Val Gln Leu Pro Ala
420 425 430
Pro Met Ser Val Val Asn Ala Gln Thr Gln Thr Ala Gln Tyr Thr Asp
435 440 445
Gly Glu Asn Ile Trp Thr Asp Thr Gly Arg Ser Trp Leu Cys Thr Leu
450 455 460
Arg Gly Tyr Cys Thr Thr Asn Cys Phe Pro Gly Arg Gly Cys Tyr Asn
465 470 475 480
Asn Ser Thr Gly Tyr Gly Glu Ser Cys Asn Gln Ser Leu Pro Gly Gln
485 490 495
Lys Ile His Ala Leu Tyr Pro Phe Thr Gln Thr Asn Val Leu Gly Gln
500 505 510
Ser Gly Lys Leu Gly Leu Leu Ala Ser His Ile Pro Tyr Asp Leu Ser
515 520 525
Pro Asn Asn Thr Ile Gly Asp Lys Asp Thr Asp Ser Thr Asn Ile Val
530 535 540
Ala Lys Gly Ile Pro Val Glu Lys Gly Tyr Ala Ser Ser Gly Gln Lys
545 550 555 560
Val Glu Ile Ile Arg Glu Trp Ile Asn Gly Ala Asn Val Val Gln Leu
565 570 575
Ser Pro Gly Gln Ser Trp Gly Met Asp Phe Thr Asn Ser Thr Gly Gly
580 585 590
Gln Tyr Met Val Arg Cys Arg Tyr Ala Ser Thr Asn Asp Thr Pro Ile
595 600 605
Phe Phe Asn Leu Val Tyr Asp Gly Gly Ser Asn Pro Ile Tyr Asn Gln
610 615 620
Met Thr Phe Pro Ala Thr Lys Glu Thr Pro Ala His Asp Ser Val Asp
625 630 635 640
Asn Lys Ile Leu Gly Ile Lys Gly Ile Asn Gly Asn Tyr Ser Leu Met
645 650 655
Asn Val Lys Asp Ser Val Glu Leu Pro Ser Gly Lys Phe His Val Phe
660 665 670
Phe Thr Asn Asn Gly Ser Ser Ala Ile Tyr Leu Asp Arg Leu Glu Phe
675 680 685
Val Pro Leu Gly Lys Pro Ser Pro Gly Val Leu Tyr Ser Gly Ser Tyr
690 695 700
Asp Leu Met Gly Ser Gln Tyr Ala Ser Val Leu Phe Asn Asp Gln Asn
705 710 715 720
Ala Ser Tyr Thr Thr Val Ser Ile Asn Gly Val Ser Asp Ala His Ser
725 730 735
Thr Ser Gly Ser Ile Thr Leu Phe Asn Asn Glu Thr Leu Val Lys Gly
740 745 750
Phe Asp Val Pro Gly Ser Gly Gln Ser Tyr Gln Tyr Ser Asn Val Thr
755 760 765
Val Pro Pro Tyr Asn Arg Val Asn Met Thr Lys Gly Thr Tyr Ala Glu
770 775 780
Leu Ser Gly Ser Val Thr Ile Lys Gly Asn
785 790
<210> 22
<211> 687
<212> PRT
<213> 苏云金芽孢杆菌
<400> 22
Met Leu Val Gly Val Phe Trp Pro Lys Gly Thr Asn Asn Gln Glu Asn
1 5 10 15
Leu Ile Thr Val Ile Asp Lys Glu Val Gln Arg Ile Leu Asp Glu Lys
20 25 30
Leu Ser Asp Gln Leu Ile Lys Lys Leu Asn Ala Asp Leu Asn Ala Phe
35 40 45
Thr Asp Leu Val Thr Arg Leu Glu Glu Val Ile Ile Asp Ala Thr Phe
50 55 60
Glu Asn His Lys Pro Val Leu Gln Val Ser Lys Ser Asn Tyr Met Lys
65 70 75 80
Val Asp Ser Ala Tyr Phe Ser Thr Gly Gly Ile Leu Thr Leu Gly Met
85 90 95
Ser Asp Phe Leu Thr Asp Thr Tyr Ser Lys Leu Thr Phe Pro Leu Tyr
100 105 110
Val Leu Gly Ala Thr Met Lys Leu Ser Ala Tyr His Ser Tyr Ile Gln
115 120 125
Phe Gly Asn Thr Trp Leu Asn Lys Val Tyr Asp Leu Ser Ser Asp Glu
130 135 140
Gly Lys Thr Met Ser Gln Ala Leu Ala Arg Ala Lys Gln His Met Arg
145 150 155 160
Gln Asp Ile Ala Phe Tyr Thr Ser Gln Ala Leu Asn Met Phe Thr Gly
165 170 175
Asn Leu Pro Ser Leu Ser Ser Asn Lys Tyr Ala Ile Asn Asp Tyr Asn
180 185 190
Val Tyr Thr Arg Ala Met Val Leu Asn Gly Leu Asp Ile Val Ala Thr
195 200 205
Trp Pro Thr Leu Tyr Pro Asp Asp Tyr Ser Ser Gln Ile Lys Leu Glu
210 215 220
Lys Thr Arg Val Ile Phe Ser Asp Met Val Gly Gln Ser Glu Ser Arg
225 230 235 240
Asp Gly Ser Val Thr Ile Lys Asn Ile Phe Asp Asn Thr Asp Ser His
245 250 255
Gln His Gly Ser Ile Gly Leu Asn Ser Ile Ser Tyr Phe Pro Asp Glu
260 265 270
Leu Gln Lys Ala Gln Leu Arg Met Tyr Asp Tyr Asn His Lys Pro Tyr
275 280 285
Cys Thr Asp Cys Phe Cys Trp Pro Tyr Gly Val Ile Leu Asn Tyr Asn
290 295 300
Lys Asn Thr Phe Arg Tyr Gly Asp Asn Asp Pro Gly Leu Ser Gly Asp
305 310 315 320
Val Gln Leu Pro Ala Pro Met Ser Val Val Asn Ala Gln Thr Gln Thr
325 330 335
Ala Gln Tyr Thr Asp Gly Glu Asn Ile Trp Thr Asp Thr Gly Arg Ser
340 345 350
Trp Leu Cys Thr Leu Arg Gly Tyr Cys Thr Thr Asn Cys Phe Pro Gly
355 360 365
Arg Gly Cys Tyr Asn Asn Ser Thr Gly Tyr Gly Glu Ser Cys Asn Gln
370 375 380
Ser Leu Pro Gly Gln Lys Ile His Ala Leu Tyr Pro Phe Thr Gln Thr
385 390 395 400
Asn Val Leu Gly Gln Ser Gly Lys Leu Gly Leu Leu Ala Ser His Ile
405 410 415
Pro Tyr Asp Leu Ser Pro Asn Asn Thr Ile Gly Asp Lys Asp Thr Asp
420 425 430
Ser Thr Asn Ile Val Ala Lys Gly Ile Pro Val Glu Lys Gly Tyr Ala
435 440 445
Ser Ser Gly Gln Lys Val Glu Ile Ile Arg Glu Trp Ile Asn Gly Ala
450 455 460
Asn Val Val Gln Leu Ser Pro Gly Gln Ser Trp Gly Met Asp Phe Thr
465 470 475 480
Asn Ser Thr Gly Gly Gln Tyr Met Val Arg Cys Arg Tyr Ala Ser Thr
485 490 495
Asn Asp Thr Pro Ile Phe Phe Asn Leu Val Tyr Asp Gly Gly Ser Asn
500 505 510
Pro Ile Tyr Asn Gln Met Thr Phe Pro Ala Thr Lys Glu Thr Pro Ala
515 520 525
His Asp Ser Val Asp Asn Lys Ile Leu Gly Ile Lys Gly Ile Asn Gly
530 535 540
Asn Tyr Ser Leu Met Asn Val Lys Asp Ser Val Glu Leu Pro Ser Gly
545 550 555 560
Lys Phe His Val Phe Phe Thr Asn Asn Gly Ser Ser Ala Ile Tyr Leu
565 570 575
Asp Arg Leu Glu Phe Val Pro Leu Gly Lys Pro Ser Pro Gly Val Leu
580 585 590
Tyr Ser Gly Ser Tyr Asp Leu Met Gly Ser Gln Tyr Ala Ser Val Leu
595 600 605
Phe Asn Asp Gln Asn Ala Ser Tyr Thr Thr Val Ser Ile Asn Gly Val
610 615 620
Ser Asp Ala His Ser Thr Ser Gly Ser Ile Thr Leu Phe Asn Asn Glu
625 630 635 640
Thr Leu Val Lys Gly Phe Asp Val Pro Gly Ser Gly Gln Ser Tyr Gln
645 650 655
Tyr Ser Asn Val Thr Val Pro Pro Tyr Asn Arg Val Asn Met Thr Lys
660 665 670
Gly Thr Tyr Ala Glu Leu Ser Gly Ser Val Thr Ile Lys Gly Asn
675 680 685
<210> 23
<211> 1173
<212> PRT
<213> 苏云金芽孢杆菌
<400> 23
Met Leu Lys Leu Arg Lys Arg Arg Tyr Phe Met Glu Gly Asn Asn Leu
1 5 10 15
Asn Gln Cys Ile Pro Tyr Asn Cys Leu Ser Asn Pro Lys Asp Ile Ile
20 25 30
Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn Thr Val Ala Asp Ile Thr
35 40 45
Leu Gly Ile Val Asn Leu Leu Phe Ser Glu Phe Val Pro Gly Gly Gly
50 55 60
Phe Ile Leu Gly Leu Leu Asp Leu Ile Trp Gly Ser Ile Gly Arg Ser
65 70 75 80
Gln Trp Asp Leu Phe Leu Glu Gln Ile Glu Gln Leu Ile Lys Gln Arg
85 90 95
Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile Ser Arg Leu Glu Gly Leu
100 105 110
Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala Phe Ser Asp Trp Glu Ala
115 120 125
Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu Met Arg Ile Gln Phe Asn
130 135 140
Asp Met Asn Ser Ala Ile Ile Thr Ala Leu Pro Leu Phe Arg Val Gln
145 150 155 160
Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr Val Gln Ala Ala Asn Leu
165 170 175
His Leu Ser Ile Leu Arg Asp Val Ser Val Phe Gly Glu Arg Trp Gly
180 185 190
Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr Ser Asp Leu Thr Ser Leu
195 200 205
Ile His Val Tyr Thr Asn His Cys Val Asp Thr Tyr Asn Gln Gly Leu
210 215 220
Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp Trp Ile Val Tyr Asn Arg
225 230 235 240
Phe Arg Arg Gln Leu Thr Ile Ser Val Leu Asp Ile Val Ala Phe Phe
245 250 255
Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile Gln Thr Ala Thr Gln Leu
260 265 270
Thr Arg Glu Ile Tyr Leu Asp Leu Pro Phe Ile Asn Glu Asn Leu Ser
275 280 285
Pro Ala Ala Ser Tyr Pro Ser Phe Ser Asp Ala Glu Ser Ala Ile Ile
290 295 300
Arg Ser Pro His Leu Val Asp Phe Leu Asn Ser Phe Thr Ile Tyr Thr
305 310 315 320
Asp Ser Leu Ala Arg Tyr Leu Tyr Trp Gly Gly His Arg Val Asn Phe
325 330 335
Thr Arg Ser Gly Val Thr Thr Phe Ile Gln Ser Pro Leu Tyr Gly Arg
340 345 350
Glu Gly Asn Ala Glu Arg Ser Val Ile Ile Ser Ala Ser Ser Ser Val
355 360 365
Pro Ile Phe Arg Thr Leu Ser Tyr Val Thr Gly Leu Asp Asn Ala Asn
370 375 380
Pro Val Ala Gly Ile Glu Gly Val Glu Phe Gln Asn Thr Ile Ser Arg
385 390 395 400
Ser Ile Tyr Arg Lys Ser Gly Pro Ile Asp Ser Phe Asn Glu Leu Pro
405 410 415
Pro Gln Asp Ala Ser Val Ser Pro Ser Ile Gly Tyr Ser His Arg Leu
420 425 430
Cys His Ala Thr Phe Leu Glu Arg Ile Ser Gly Pro Arg Ile Ala Gly
435 440 445
Val Val Phe Ser Trp Thr His Arg Ser Ala Ser Pro Thr Asn Glu Val
450 455 460
Ser Ser Ser Arg Ile Thr Gln Ile Pro Trp Val Lys Ala His Thr Leu
465 470 475 480
Ala Ser Gly Ala Ser Val Ile Lys Gly Pro Gly Phe Thr Gly Gly Asp
485 490 495
Ile Leu Thr Arg Asn Thr Leu Gly Glu Leu Gly Thr Leu Arg Val Thr
500 505 510
Phe Ala Gly Arg Leu Ser Gln Ser Tyr Tyr Ile Arg Phe Arg Tyr Ala
515 520 525
Ser Val Ala Asn Arg Ser Gly Ile Phe Ser Tyr Ser Gln Pro Thr Ser
530 535 540
Tyr Gly Ile Ser Phe Pro Lys Thr Met Asp Ala Asn Glu Ser Leu Thr
545 550 555 560
Ser Arg Ser Phe Ala Leu Ala Thr Leu Ala Thr Pro Leu Thr Phe Arg
565 570 575
Arg Gln Glu Glu Leu Asn Leu Gln Ile Pro Ser Gly Thr Tyr Ile Asp
580 585 590
Arg Ile Glu Phe Val Pro Val Asp Glu Thr Phe Thr Thr Glu Ser Asp
595 600 605
Leu Asp Arg Ala Gln Gln Ala Val Asn Ala Leu Phe Thr Ser Ser Asn
610 615 620
Gln Ile Gly Leu Lys Thr Asp Val Thr Asp Tyr His Ile Asp Gln Val
625 630 635 640
Ser Asn Leu Val Asp Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys
645 650 655
Lys Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg Leu Ser Asp Glu
660 665 670
Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg Gly Ile Asn Arg Gln Leu
675 680 685
Asp Arg Gly Trp Ser Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly Asp
690 695 700
Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Pro Gly Thr Phe Asp Glu
705 710 715 720
Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys
725 730 735
Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Gly Asp Ser Gln Asp
740 745 750
Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His Glu Ile Val Asn
755 760 765
Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Val Glu Asn Ser Ile
770 775 780
Gly Pro Cys Gly Glu Pro Asn Arg Cys Ala Pro His Leu Glu Trp Asn
785 790 795 800
Pro Asn Leu Glu Cys Ser Cys Arg Glu Gly Glu Lys Cys Ala His His
805 810 815
Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn
820 825 830
Glu Asp Leu Gly Val Trp Ala Ile Phe Lys Ile Lys Thr Gln Asp Gly
835 840 845
His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val
850 855 860
Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp
865 870 875 880
Lys Arg Glu Lys Leu Glu Leu Glu Thr Asn Ile Val Tyr Lys Glu Ala
885 890 895
Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu
900 905 910
Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val
915 920 925
His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Ile Ile Pro Gly
930 935 940
Val Asn Ala Gly Ile Phe Glu Glu Leu Glu Gly Arg Ile Tyr Thr Ala
945 950 955 960
Tyr Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asp
965 970 975
Asn Gly Leu Leu Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu
980 985 990
Gln Asn Asn His Arg Ser Val Leu Val Ile Pro Glu Trp Glu Ala Glu
995 1000 1005
Val Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu
1010 1015 1020
Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr
1025 1030 1035
Ile His Glu Ile Glu Asp Asn Thr Asp Glu Leu Lys Phe Ser Asn
1040 1045 1050
Cys Val Glu Glu Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn
1055 1060 1065
Asp Tyr Thr Ala Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser
1070 1075 1080
Arg Asn Arg Gly Tyr Asp Gly Ala Tyr Glu Ser Asn Ser Ser Val
1085 1090 1095
Pro Ala Asp Tyr Ala Ser Ala Tyr Glu Glu Lys Ala Tyr Thr Asp
1100 1105 1110
Gly Arg Arg Glu Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp
1115 1120 1125
Tyr Ala Pro Leu Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr
1130 1135 1140
Phe Pro Glu Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu
1145 1150 1155
Gly Thr Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu
1160 1165 1170
<210> 24
<211> 1163
<212> PRT
<213> 苏云金芽孢杆菌
<400> 24
Met Glu Gly Asn Asn Leu Asn Gln Cys Ile Pro Tyr Asn Cys Leu Ser
1 5 10 15
Asn Pro Lys Asp Ile Ile Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn
20 25 30
Thr Val Ala Asp Ile Thr Leu Gly Ile Val Asn Leu Leu Phe Ser Glu
35 40 45
Phe Val Pro Gly Gly Gly Phe Ile Leu Gly Leu Leu Asp Leu Ile Trp
50 55 60
Gly Ser Ile Gly Arg Ser Gln Trp Asp Leu Phe Leu Glu Gln Ile Glu
65 70 75 80
Gln Leu Ile Lys Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95
Ser Arg Leu Glu Gly Leu Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala
100 105 110
Phe Ser Asp Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu
115 120 125
Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Ile Ile Thr Ala Leu
130 135 140
Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr
145 150 155 160
Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val
165 170 175
Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr
180 185 190
Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp
195 200 205
Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp
210 215 220
Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu
225 230 235 240
Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile
245 250 255
Gln Thr Ala Thr Gln Leu Thr Arg Glu Ile Tyr Leu Asp Leu Pro Phe
260 265 270
Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Ser Phe Ser Asp
275 280 285
Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn
290 295 300
Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Leu Tyr Trp Gly
305 310 315 320
Gly His Arg Val Asn Phe Thr Arg Ser Gly Val Thr Thr Phe Ile Gln
325 330 335
Ser Pro Leu Tyr Gly Arg Glu Gly Asn Ala Glu Arg Ser Val Ile Ile
340 345 350
Ser Ala Ser Ser Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Val Thr
355 360 365
Gly Leu Asp Asn Ala Asn Pro Val Ala Gly Ile Glu Gly Val Glu Phe
370 375 380
Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile Asp
385 390 395 400
Ser Phe Asn Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ser Ile
405 410 415
Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile Ser
420 425 430
Gly Pro Arg Ile Ala Gly Val Val Phe Ser Trp Thr His Arg Ser Ala
435 440 445
Ser Pro Thr Asn Glu Val Ser Ser Ser Arg Ile Thr Gln Ile Pro Trp
450 455 460
Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly Pro
465 470 475 480
Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Thr Leu Gly Glu Leu
485 490 495
Gly Thr Leu Arg Val Thr Phe Ala Gly Arg Leu Ser Gln Ser Tyr Tyr
500 505 510
Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Ile Phe Ser
515 520 525
Tyr Ser Gln Pro Thr Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met Asp
530 535 540
Ala Asn Glu Ser Leu Thr Ser Arg Ser Phe Ala Leu Ala Thr Leu Ala
545 550 555 560
Thr Pro Leu Thr Phe Arg Arg Gln Glu Glu Leu Asn Leu Gln Ile Pro
565 570 575
Ser Gly Thr Tyr Ile Asp Arg Ile Glu Phe Val Pro Val Asp Glu Thr
580 585 590
Phe Thr Thr Glu Ser Asp Leu Asp Arg Ala Gln Gln Ala Val Asn Ala
595 600 605
Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp
610 615 620
Tyr His Ile Asp Gln Val Ser Asn Leu Val Asp Cys Leu Ser Asp Glu
625 630 635 640
Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys His Ala
645 650 655
Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg
660 665 670
Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Ser Gly Ser Thr Asp Ile
675 680 685
Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu
690 695 700
Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile
705 710 715 720
Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr
725 730 735
Ile Gly Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala
740 745 750
Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu
755 760 765
Ser Val Glu Asn Ser Ile Gly Pro Cys Gly Glu Pro Asn Arg Cys Ala
770 775 780
Pro His Leu Glu Trp Asn Pro Asn Leu Glu Cys Ser Cys Arg Glu Gly
785 790 795 800
Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp Val
805 810 815
Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Ala Ile Phe Lys
820 825 830
Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu
835 840 845
Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala
850 855 860
Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Leu Glu Thr Asn
865 870 875 880
Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn
885 890 895
Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His
900 905 910
Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu
915 920 925
Leu Ser Ile Ile Pro Gly Val Asn Ala Gly Ile Phe Glu Glu Leu Glu
930 935 940
Gly Arg Ile Tyr Thr Ala Tyr Ser Leu Tyr Asp Ala Arg Asn Val Ile
945 950 955 960
Lys Asn Gly Asp Phe Asp Asn Gly Leu Leu Cys Trp Asn Val Lys Gly
965 970 975
His Val Asp Val Glu Glu Gln Asn Asn His Arg Ser Val Leu Val Ile
980 985 990
Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val Cys Pro Gly
995 1000 1005
Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly
1010 1015 1020
Glu Gly Cys Val Thr Ile His Glu Ile Glu Asp Asn Thr Asp Glu
1025 1030 1035
Leu Lys Phe Ser Asn Cys Val Glu Glu Glu Val Tyr Pro Asn Asn
1040 1045 1050
Thr Val Thr Cys Asn Asp Tyr Thr Ala Thr Gln Glu Glu Tyr Glu
1055 1060 1065
Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Gly Ala Tyr Glu
1070 1075 1080
Ser Asn Ser Ser Val Pro Ala Asp Tyr Ala Ser Ala Tyr Glu Glu
1085 1090 1095
Lys Ala Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu Ser Asn
1100 1105 1110
Arg Gly Tyr Gly Asp Tyr Ala Pro Leu Pro Ala Gly Tyr Val Thr
1115 1120 1125
Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp Ile Glu
1130 1135 1140
Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val Glu Leu
1145 1150 1155
Leu Leu Met Glu Glu
1160
<210> 25
<211> 599
<212> PRT
<213> 苏云金芽孢杆菌
<400> 25
Met Leu Lys Leu Arg Lys Arg Arg Tyr Phe Met Glu Gly Asn Asn Leu
1 5 10 15
Asn Gln Cys Ile Pro Tyr Asn Cys Leu Ser Asn Pro Lys Asp Ile Ile
20 25 30
Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn Thr Val Ala Asp Ile Thr
35 40 45
Leu Gly Ile Val Asn Leu Leu Phe Ser Glu Phe Val Pro Gly Gly Gly
50 55 60
Phe Ile Leu Gly Leu Leu Asp Leu Ile Trp Gly Ser Ile Gly Arg Ser
65 70 75 80
Gln Trp Asp Leu Phe Leu Glu Gln Ile Glu Gln Leu Ile Lys Gln Arg
85 90 95
Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile Ser Arg Leu Glu Gly Leu
100 105 110
Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala Phe Ser Asp Trp Glu Ala
115 120 125
Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu Met Arg Ile Gln Phe Asn
130 135 140
Asp Met Asn Ser Ala Ile Ile Thr Ala Leu Pro Leu Phe Arg Val Gln
145 150 155 160
Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr Val Gln Ala Ala Asn Leu
165 170 175
His Leu Ser Ile Leu Arg Asp Val Ser Val Phe Gly Glu Arg Trp Gly
180 185 190
Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr Ser Asp Leu Thr Ser Leu
195 200 205
Ile His Val Tyr Thr Asn His Cys Val Asp Thr Tyr Asn Gln Gly Leu
210 215 220
Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp Trp Ile Val Tyr Asn Arg
225 230 235 240
Phe Arg Arg Gln Leu Thr Ile Ser Val Leu Asp Ile Val Ala Phe Phe
245 250 255
Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile Gln Thr Ala Thr Gln Leu
260 265 270
Thr Arg Glu Ile Tyr Leu Asp Leu Pro Phe Ile Asn Glu Asn Leu Ser
275 280 285
Pro Ala Ala Ser Tyr Pro Ser Phe Ser Asp Ala Glu Ser Ala Ile Ile
290 295 300
Arg Ser Pro His Leu Val Asp Phe Leu Asn Ser Phe Thr Ile Tyr Thr
305 310 315 320
Asp Ser Leu Ala Arg Tyr Leu Tyr Trp Gly Gly His Arg Val Asn Phe
325 330 335
Thr Arg Ser Gly Val Thr Thr Phe Ile Gln Ser Pro Leu Tyr Gly Arg
340 345 350
Glu Gly Asn Ala Glu Arg Ser Val Ile Ile Ser Ala Ser Ser Ser Val
355 360 365
Pro Ile Phe Arg Thr Leu Ser Tyr Val Thr Gly Leu Asp Asn Ala Asn
370 375 380
Pro Val Ala Gly Ile Glu Gly Val Glu Phe Gln Asn Thr Ile Ser Arg
385 390 395 400
Ser Ile Tyr Arg Lys Ser Gly Pro Ile Asp Ser Phe Asn Glu Leu Pro
405 410 415
Pro Gln Asp Ala Ser Val Ser Pro Ser Ile Gly Tyr Ser His Arg Leu
420 425 430
Cys His Ala Thr Phe Leu Glu Arg Ile Ser Gly Pro Arg Ile Ala Gly
435 440 445
Val Val Phe Ser Trp Thr His Arg Ser Ala Ser Pro Thr Asn Glu Val
450 455 460
Ser Ser Ser Arg Ile Thr Gln Ile Pro Trp Val Lys Ala His Thr Leu
465 470 475 480
Ala Ser Gly Ala Ser Val Ile Lys Gly Pro Gly Phe Thr Gly Gly Asp
485 490 495
Ile Leu Thr Arg Asn Thr Leu Gly Glu Leu Gly Thr Leu Arg Val Thr
500 505 510
Phe Ala Gly Arg Leu Ser Gln Ser Tyr Tyr Ile Arg Phe Arg Tyr Ala
515 520 525
Ser Val Ala Asn Arg Ser Gly Ile Phe Ser Tyr Ser Gln Pro Thr Ser
530 535 540
Tyr Gly Ile Ser Phe Pro Lys Thr Met Asp Ala Asn Glu Ser Leu Thr
545 550 555 560
Ser Arg Ser Phe Ala Leu Ala Thr Leu Ala Thr Pro Leu Thr Phe Arg
565 570 575
Arg Gln Glu Glu Leu Asn Leu Gln Ile Pro Ser Gly Thr Tyr Ile Asp
580 585 590
Arg Ile Glu Phe Val Pro Val
595
<210> 26
<211> 589
<212> PRT
<213> 苏云金芽孢杆菌
<400> 26
Met Glu Gly Asn Asn Leu Asn Gln Cys Ile Pro Tyr Asn Cys Leu Ser
1 5 10 15
Asn Pro Lys Asp Ile Ile Leu Gly Asp Glu Arg Leu Glu Thr Gly Asn
20 25 30
Thr Val Ala Asp Ile Thr Leu Gly Ile Val Asn Leu Leu Phe Ser Glu
35 40 45
Phe Val Pro Gly Gly Gly Phe Ile Leu Gly Leu Leu Asp Leu Ile Trp
50 55 60
Gly Ser Ile Gly Arg Ser Gln Trp Asp Leu Phe Leu Glu Gln Ile Glu
65 70 75 80
Gln Leu Ile Lys Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95
Ser Arg Leu Glu Gly Leu Ser Asp Leu Tyr Lys Thr Tyr Ala Arg Ala
100 105 110
Phe Ser Asp Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu
115 120 125
Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Ile Ile Thr Ala Leu
130 135 140
Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr
145 150 155 160
Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val
165 170 175
Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr
180 185 190
Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp
195 200 205
Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Thr Asp
210 215 220
Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu
225 230 235 240
Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile
245 250 255
Gln Thr Ala Thr Gln Leu Thr Arg Glu Ile Tyr Leu Asp Leu Pro Phe
260 265 270
Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Ser Phe Ser Asp
275 280 285
Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn
290 295 300
Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Leu Tyr Trp Gly
305 310 315 320
Gly His Arg Val Asn Phe Thr Arg Ser Gly Val Thr Thr Phe Ile Gln
325 330 335
Ser Pro Leu Tyr Gly Arg Glu Gly Asn Ala Glu Arg Ser Val Ile Ile
340 345 350
Ser Ala Ser Ser Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Val Thr
355 360 365
Gly Leu Asp Asn Ala Asn Pro Val Ala Gly Ile Glu Gly Val Glu Phe
370 375 380
Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile Asp
385 390 395 400
Ser Phe Asn Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ser Ile
405 410 415
Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile Ser
420 425 430
Gly Pro Arg Ile Ala Gly Val Val Phe Ser Trp Thr His Arg Ser Ala
435 440 445
Ser Pro Thr Asn Glu Val Ser Ser Ser Arg Ile Thr Gln Ile Pro Trp
450 455 460
Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly Pro
465 470 475 480
Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Thr Leu Gly Glu Leu
485 490 495
Gly Thr Leu Arg Val Thr Phe Ala Gly Arg Leu Ser Gln Ser Tyr Tyr
500 505 510
Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Ile Phe Ser
515 520 525
Tyr Ser Gln Pro Thr Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met Asp
530 535 540
Ala Asn Glu Ser Leu Thr Ser Arg Ser Phe Ala Leu Ala Thr Leu Ala
545 550 555 560
Thr Pro Leu Thr Phe Arg Arg Gln Glu Glu Leu Asn Leu Gln Ile Pro
565 570 575
Ser Gly Thr Tyr Ile Asp Arg Ile Glu Phe Val Pro Val
580 585
<210> 27
<211> 655
<212> PRT
<213> 苏云金芽孢杆菌
<400> 27
Met Thr His Asn Asp Asn Asn Asn Lys Phe Glu Ile Lys Asp Thr Gly
1 5 10 15
Thr Lys Pro Arg Ser Pro Leu Ala Asn Ala Pro Gly Pro Thr Trp Gln
20 25 30
Asn Ile Asn Asn Arg Asp Val Glu Thr Phe Gly Ser Ile Glu Ile Ala
35 40 45
Gly Lys Val Val Ser Gly Val Ile Ser Ser Val Ile Lys Ser Leu Arg
50 55 60
Gln Gln Ala Gln Ile Asp Lys Ile Val Ala Ile Val Val Val Glu Val
65 70 75 80
Phe Glu Val Leu Trp Pro Val Leu Glu Gly Met Trp Tyr Ala Met Met
85 90 95
Asp Ala Val Glu Ile Met Ile Gln Glu Ala Ile Thr Thr Ala Val Arg
100 105 110
Ser Lys Ala Gln Ala Glu Leu Asn Gly Ile Arg Asn Ala Leu Val Leu
115 120 125
Phe Gln Gln Ala Phe Asp Asp Trp Glu Lys Asn Ser Asp Asn Pro Gln
130 135 140
Leu Gln Asp Arg Val Arg Arg Gln Phe Thr Ala Thr Asn Thr Leu Ile
145 150 155 160
Gln Phe Ala Met Ser Ser Phe Ala Val Pro Gly Phe Gln Val Pro Leu
165 170 175
Leu Val Val Tyr Ala Gln Ala Ala Asn Leu His Leu Leu Phe Leu Arg
180 185 190
Glu Ala Val Val Leu Gly Glu Lys Trp Gly Met Ser Arg Glu Glu Val
195 200 205
Asp Asp Tyr Tyr Asn Gly Glu Leu Gly Leu Thr Glu Leu Thr Gln Ser
210 215 220
Tyr Thr Asn His Cys Thr Asn Trp Tyr His Glu Gly Leu Ala Gln Ser
225 230 235 240
Met Lys Leu Asn Pro Ser Val Thr Ile Leu Glu Gln Trp Asn Leu Tyr
245 250 255
Asn Asp Phe Arg Arg Glu Met Thr Ile Met Ile Leu Asp Ile Val Ala
260 265 270
Leu Trp Pro Thr Tyr Asp Val Lys Leu Tyr Pro Ala Gly Thr Lys Thr
275 280 285
Glu Leu Thr Arg Ile Ile Tyr Thr Pro Leu Met Gly Val Leu Glu Asp
290 295 300
Ser Ser Ser Ile Ser Ala Thr Arg Lys Glu Tyr Gly Asn Ile Ala Ala
305 310 315 320
Ile Asn Gln Gln Asp Gly Ala Thr Thr Ile Pro Pro Ala Leu Phe Ile
325 330 335
Trp Leu Glu Lys Gln Ile Val Tyr Pro Tyr Asn Asn Leu Ile Tyr Ser
340 345 350
Tyr Gln Asn Phe Gln Lys Thr Thr Phe Gly Lys Val Met Asn Gly Thr
355 360 365
Ile Phe Gly Ser Asn Ser Glu Lys His Leu Thr Asn Pro Ile Ser Ile
370 375 380
Pro Ile Asp Ala Glu Ser Tyr Asp Val Tyr Lys Val Asp Thr Ser Tyr
385 390 395 400
Ser Ala Lys Met Glu Ile Lys Ser Ser Pro Ile His Lys Leu Val Tyr
405 410 415
Tyr Arg Ser Lys Glu Gln Gln Glu Ser Ile Ile Ser Thr Asn Thr Ser
420 425 430
Lys Gly Pro Ile Asp Gln Val Ser Glu Ile Ala Asn Glu Gly Tyr Gln
435 440 445
Asp Tyr Ser His Cys Leu Ala His Met Ala Gly Trp Val Ser Ile Ala
450 455 460
Tyr Gly Thr Gly Leu Thr Glu Lys Pro Tyr Leu Val Pro Tyr Asn Leu
465 470 475 480
Ala Leu Gly Trp Thr Tyr Ala Asn Val Asp Pro Val Asn Ser Ile Ala
485 490 495
Pro Asp Ala Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Lys Val Ile
500 505 510
Gly Ile Pro Glu Glu Glu Ala Ile Leu Gly Glu Val Thr Ala Ile Gln
515 520 525
Gly Pro Gly Phe Thr Gly Gly Asn Leu Val Gly Leu Phe Ala Gly Ala
530 535 540
Glu Leu His Met Lys Val Thr Asn Pro Val Ser Asn Val Ala Gly Tyr
545 550 555 560
Gln Met Arg Ile Arg Tyr Ala Asn Asn His Pro Thr Ile Leu Ala Val
565 570 575
Ser Tyr Gln Gly Val Glu Thr Ser Ser Gly Lys Phe Asp Val Pro Val
580 585 590
Thr Tyr Ser Gly Asp Phe Lys Thr Lys Leu Thr Tyr Asn Ala Phe Lys
595 600 605
Phe Lys Glu Ala Ile Ile Ile Pro Ser Pro Leu Arg Glu Glu Ile Ala
610 615 620
Asp Ile Val Leu Arg Asn Glu Gly Asp Ser Asn Leu Leu Ile Asp Lys
625 630 635 640
Ile Glu Leu Ile Pro Met Asp Phe Trp Arg His Glu Gln Cys Asn
645 650 655
<210> 28
<211> 565
<212> PRT
<213> 苏云金芽孢杆菌
<400> 28
Met Trp Tyr Ala Met Met Asp Ala Val Glu Ile Met Ile Gln Glu Ala
1 5 10 15
Ile Thr Thr Ala Val Arg Ser Lys Ala Gln Ala Glu Leu Asn Gly Ile
20 25 30
Arg Asn Ala Leu Val Leu Phe Gln Gln Ala Phe Asp Asp Trp Glu Lys
35 40 45
Asn Ser Asp Asn Pro Gln Leu Gln Asp Arg Val Arg Arg Gln Phe Thr
50 55 60
Ala Thr Asn Thr Leu Ile Gln Phe Ala Met Ser Ser Phe Ala Val Pro
65 70 75 80
Gly Phe Gln Val Pro Leu Leu Val Val Tyr Ala Gln Ala Ala Asn Leu
85 90 95
His Leu Leu Phe Leu Arg Glu Ala Val Val Leu Gly Glu Lys Trp Gly
100 105 110
Met Ser Arg Glu Glu Val Asp Asp Tyr Tyr Asn Gly Glu Leu Gly Leu
115 120 125
Thr Glu Leu Thr Gln Ser Tyr Thr Asn His Cys Thr Asn Trp Tyr His
130 135 140
Glu Gly Leu Ala Gln Ser Met Lys Leu Asn Pro Ser Val Thr Ile Leu
145 150 155 160
Glu Gln Trp Asn Leu Tyr Asn Asp Phe Arg Arg Glu Met Thr Ile Met
165 170 175
Ile Leu Asp Ile Val Ala Leu Trp Pro Thr Tyr Asp Val Lys Leu Tyr
180 185 190
Pro Ala Gly Thr Lys Thr Glu Leu Thr Arg Ile Ile Tyr Thr Pro Leu
195 200 205
Met Gly Val Leu Glu Asp Ser Ser Ser Ile Ser Ala Thr Arg Lys Glu
210 215 220
Tyr Gly Asn Ile Ala Ala Ile Asn Gln Gln Asp Gly Ala Thr Thr Ile
225 230 235 240
Pro Pro Ala Leu Phe Ile Trp Leu Glu Lys Gln Ile Val Tyr Pro Tyr
245 250 255
Asn Asn Leu Ile Tyr Ser Tyr Gln Asn Phe Gln Lys Thr Thr Phe Gly
260 265 270
Lys Val Met Asn Gly Thr Ile Phe Gly Ser Asn Ser Glu Lys His Leu
275 280 285
Thr Asn Pro Ile Ser Ile Pro Ile Asp Ala Glu Ser Tyr Asp Val Tyr
290 295 300
Lys Val Asp Thr Ser Tyr Ser Ala Lys Met Glu Ile Lys Ser Ser Pro
305 310 315 320
Ile His Lys Leu Val Tyr Tyr Arg Ser Lys Glu Gln Gln Glu Ser Ile
325 330 335
Ile Ser Thr Asn Thr Ser Lys Gly Pro Ile Asp Gln Val Ser Glu Ile
340 345 350
Ala Asn Glu Gly Tyr Gln Asp Tyr Ser His Cys Leu Ala His Met Ala
355 360 365
Gly Trp Val Ser Ile Ala Tyr Gly Thr Gly Leu Thr Glu Lys Pro Tyr
370 375 380
Leu Val Pro Tyr Asn Leu Ala Leu Gly Trp Thr Tyr Ala Asn Val Asp
385 390 395 400
Pro Val Asn Ser Ile Ala Pro Asp Ala Ile Thr Gln Ile Pro Ala Val
405 410 415
Lys Gly Asp Lys Val Ile Gly Ile Pro Glu Glu Glu Ala Ile Leu Gly
420 425 430
Glu Val Thr Ala Ile Gln Gly Pro Gly Phe Thr Gly Gly Asn Leu Val
435 440 445
Gly Leu Phe Ala Gly Ala Glu Leu His Met Lys Val Thr Asn Pro Val
450 455 460
Ser Asn Val Ala Gly Tyr Gln Met Arg Ile Arg Tyr Ala Asn Asn His
465 470 475 480
Pro Thr Ile Leu Ala Val Ser Tyr Gln Gly Val Glu Thr Ser Ser Gly
485 490 495
Lys Phe Asp Val Pro Val Thr Tyr Ser Gly Asp Phe Lys Thr Lys Leu
500 505 510
Thr Tyr Asn Ala Phe Lys Phe Lys Glu Ala Ile Ile Ile Pro Ser Pro
515 520 525
Leu Arg Glu Glu Ile Ala Asp Ile Val Leu Arg Asn Glu Gly Asp Ser
530 535 540
Asn Leu Leu Ile Asp Lys Ile Glu Leu Ile Pro Met Asp Phe Trp Arg
545 550 555 560
His Glu Gln Cys Asn
565
<210> 29
<211> 561
<212> PRT
<213> 苏云金芽孢杆菌
<400> 29
Met Met Asp Ala Val Glu Ile Met Ile Gln Glu Ala Ile Thr Thr Ala
1 5 10 15
Val Arg Ser Lys Ala Gln Ala Glu Leu Asn Gly Ile Arg Asn Ala Leu
20 25 30
Val Leu Phe Gln Gln Ala Phe Asp Asp Trp Glu Lys Asn Ser Asp Asn
35 40 45
Pro Gln Leu Gln Asp Arg Val Arg Arg Gln Phe Thr Ala Thr Asn Thr
50 55 60
Leu Ile Gln Phe Ala Met Ser Ser Phe Ala Val Pro Gly Phe Gln Val
65 70 75 80
Pro Leu Leu Val Val Tyr Ala Gln Ala Ala Asn Leu His Leu Leu Phe
85 90 95
Leu Arg Glu Ala Val Val Leu Gly Glu Lys Trp Gly Met Ser Arg Glu
100 105 110
Glu Val Asp Asp Tyr Tyr Asn Gly Glu Leu Gly Leu Thr Glu Leu Thr
115 120 125
Gln Ser Tyr Thr Asn His Cys Thr Asn Trp Tyr His Glu Gly Leu Ala
130 135 140
Gln Ser Met Lys Leu Asn Pro Ser Val Thr Ile Leu Glu Gln Trp Asn
145 150 155 160
Leu Tyr Asn Asp Phe Arg Arg Glu Met Thr Ile Met Ile Leu Asp Ile
165 170 175
Val Ala Leu Trp Pro Thr Tyr Asp Val Lys Leu Tyr Pro Ala Gly Thr
180 185 190
Lys Thr Glu Leu Thr Arg Ile Ile Tyr Thr Pro Leu Met Gly Val Leu
195 200 205
Glu Asp Ser Ser Ser Ile Ser Ala Thr Arg Lys Glu Tyr Gly Asn Ile
210 215 220
Ala Ala Ile Asn Gln Gln Asp Gly Ala Thr Thr Ile Pro Pro Ala Leu
225 230 235 240
Phe Ile Trp Leu Glu Lys Gln Ile Val Tyr Pro Tyr Asn Asn Leu Ile
245 250 255
Tyr Ser Tyr Gln Asn Phe Gln Lys Thr Thr Phe Gly Lys Val Met Asn
260 265 270
Gly Thr Ile Phe Gly Ser Asn Ser Glu Lys His Leu Thr Asn Pro Ile
275 280 285
Ser Ile Pro Ile Asp Ala Glu Ser Tyr Asp Val Tyr Lys Val Asp Thr
290 295 300
Ser Tyr Ser Ala Lys Met Glu Ile Lys Ser Ser Pro Ile His Lys Leu
305 310 315 320
Val Tyr Tyr Arg Ser Lys Glu Gln Gln Glu Ser Ile Ile Ser Thr Asn
325 330 335
Thr Ser Lys Gly Pro Ile Asp Gln Val Ser Glu Ile Ala Asn Glu Gly
340 345 350
Tyr Gln Asp Tyr Ser His Cys Leu Ala His Met Ala Gly Trp Val Ser
355 360 365
Ile Ala Tyr Gly Thr Gly Leu Thr Glu Lys Pro Tyr Leu Val Pro Tyr
370 375 380
Asn Leu Ala Leu Gly Trp Thr Tyr Ala Asn Val Asp Pro Val Asn Ser
385 390 395 400
Ile Ala Pro Asp Ala Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Lys
405 410 415
Val Ile Gly Ile Pro Glu Glu Glu Ala Ile Leu Gly Glu Val Thr Ala
420 425 430
Ile Gln Gly Pro Gly Phe Thr Gly Gly Asn Leu Val Gly Leu Phe Ala
435 440 445
Gly Ala Glu Leu His Met Lys Val Thr Asn Pro Val Ser Asn Val Ala
450 455 460
Gly Tyr Gln Met Arg Ile Arg Tyr Ala Asn Asn His Pro Thr Ile Leu
465 470 475 480
Ala Val Ser Tyr Gln Gly Val Glu Thr Ser Ser Gly Lys Phe Asp Val
485 490 495
Pro Val Thr Tyr Ser Gly Asp Phe Lys Thr Lys Leu Thr Tyr Asn Ala
500 505 510
Phe Lys Phe Lys Glu Ala Ile Ile Ile Pro Ser Pro Leu Arg Glu Glu
515 520 525
Ile Ala Asp Ile Val Leu Arg Asn Glu Gly Asp Ser Asn Leu Leu Ile
530 535 540
Asp Lys Ile Glu Leu Ile Pro Met Asp Phe Trp Arg His Glu Gln Cys
545 550 555 560
Asn
<210> 30
<211> 560
<212> PRT
<213> 苏云金芽孢杆菌
<400> 30
Met Asp Ala Val Glu Ile Met Ile Gln Glu Ala Ile Thr Thr Ala Val
1 5 10 15
Arg Ser Lys Ala Gln Ala Glu Leu Asn Gly Ile Arg Asn Ala Leu Val
20 25 30
Leu Phe Gln Gln Ala Phe Asp Asp Trp Glu Lys Asn Ser Asp Asn Pro
35 40 45
Gln Leu Gln Asp Arg Val Arg Arg Gln Phe Thr Ala Thr Asn Thr Leu
50 55 60
Ile Gln Phe Ala Met Ser Ser Phe Ala Val Pro Gly Phe Gln Val Pro
65 70 75 80
Leu Leu Val Val Tyr Ala Gln Ala Ala Asn Leu His Leu Leu Phe Leu
85 90 95
Arg Glu Ala Val Val Leu Gly Glu Lys Trp Gly Met Ser Arg Glu Glu
100 105 110
Val Asp Asp Tyr Tyr Asn Gly Glu Leu Gly Leu Thr Glu Leu Thr Gln
115 120 125
Ser Tyr Thr Asn His Cys Thr Asn Trp Tyr His Glu Gly Leu Ala Gln
130 135 140
Ser Met Lys Leu Asn Pro Ser Val Thr Ile Leu Glu Gln Trp Asn Leu
145 150 155 160
Tyr Asn Asp Phe Arg Arg Glu Met Thr Ile Met Ile Leu Asp Ile Val
165 170 175
Ala Leu Trp Pro Thr Tyr Asp Val Lys Leu Tyr Pro Ala Gly Thr Lys
180 185 190
Thr Glu Leu Thr Arg Ile Ile Tyr Thr Pro Leu Met Gly Val Leu Glu
195 200 205
Asp Ser Ser Ser Ile Ser Ala Thr Arg Lys Glu Tyr Gly Asn Ile Ala
210 215 220
Ala Ile Asn Gln Gln Asp Gly Ala Thr Thr Ile Pro Pro Ala Leu Phe
225 230 235 240
Ile Trp Leu Glu Lys Gln Ile Val Tyr Pro Tyr Asn Asn Leu Ile Tyr
245 250 255
Ser Tyr Gln Asn Phe Gln Lys Thr Thr Phe Gly Lys Val Met Asn Gly
260 265 270
Thr Ile Phe Gly Ser Asn Ser Glu Lys His Leu Thr Asn Pro Ile Ser
275 280 285
Ile Pro Ile Asp Ala Glu Ser Tyr Asp Val Tyr Lys Val Asp Thr Ser
290 295 300
Tyr Ser Ala Lys Met Glu Ile Lys Ser Ser Pro Ile His Lys Leu Val
305 310 315 320
Tyr Tyr Arg Ser Lys Glu Gln Gln Glu Ser Ile Ile Ser Thr Asn Thr
325 330 335
Ser Lys Gly Pro Ile Asp Gln Val Ser Glu Ile Ala Asn Glu Gly Tyr
340 345 350
Gln Asp Tyr Ser His Cys Leu Ala His Met Ala Gly Trp Val Ser Ile
355 360 365
Ala Tyr Gly Thr Gly Leu Thr Glu Lys Pro Tyr Leu Val Pro Tyr Asn
370 375 380
Leu Ala Leu Gly Trp Thr Tyr Ala Asn Val Asp Pro Val Asn Ser Ile
385 390 395 400
Ala Pro Asp Ala Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Lys Val
405 410 415
Ile Gly Ile Pro Glu Glu Glu Ala Ile Leu Gly Glu Val Thr Ala Ile
420 425 430
Gln Gly Pro Gly Phe Thr Gly Gly Asn Leu Val Gly Leu Phe Ala Gly
435 440 445
Ala Glu Leu His Met Lys Val Thr Asn Pro Val Ser Asn Val Ala Gly
450 455 460
Tyr Gln Met Arg Ile Arg Tyr Ala Asn Asn His Pro Thr Ile Leu Ala
465 470 475 480
Val Ser Tyr Gln Gly Val Glu Thr Ser Ser Gly Lys Phe Asp Val Pro
485 490 495
Val Thr Tyr Ser Gly Asp Phe Lys Thr Lys Leu Thr Tyr Asn Ala Phe
500 505 510
Lys Phe Lys Glu Ala Ile Ile Ile Pro Ser Pro Leu Arg Glu Glu Ile
515 520 525
Ala Asp Ile Val Leu Arg Asn Glu Gly Asp Ser Asn Leu Leu Ile Asp
530 535 540
Lys Ile Glu Leu Ile Pro Met Asp Phe Trp Arg His Glu Gln Cys Asn
545 550 555 560
<210> 31
<211> 554
<212> PRT
<213> 苏云金芽孢杆菌
<400> 31
Met Ile Gln Glu Ala Ile Thr Thr Ala Val Arg Ser Lys Ala Gln Ala
1 5 10 15
Glu Leu Asn Gly Ile Arg Asn Ala Leu Val Leu Phe Gln Gln Ala Phe
20 25 30
Asp Asp Trp Glu Lys Asn Ser Asp Asn Pro Gln Leu Gln Asp Arg Val
35 40 45
Arg Arg Gln Phe Thr Ala Thr Asn Thr Leu Ile Gln Phe Ala Met Ser
50 55 60
Ser Phe Ala Val Pro Gly Phe Gln Val Pro Leu Leu Val Val Tyr Ala
65 70 75 80
Gln Ala Ala Asn Leu His Leu Leu Phe Leu Arg Glu Ala Val Val Leu
85 90 95
Gly Glu Lys Trp Gly Met Ser Arg Glu Glu Val Asp Asp Tyr Tyr Asn
100 105 110
Gly Glu Leu Gly Leu Thr Glu Leu Thr Gln Ser Tyr Thr Asn His Cys
115 120 125
Thr Asn Trp Tyr His Glu Gly Leu Ala Gln Ser Met Lys Leu Asn Pro
130 135 140
Ser Val Thr Ile Leu Glu Gln Trp Asn Leu Tyr Asn Asp Phe Arg Arg
145 150 155 160
Glu Met Thr Ile Met Ile Leu Asp Ile Val Ala Leu Trp Pro Thr Tyr
165 170 175
Asp Val Lys Leu Tyr Pro Ala Gly Thr Lys Thr Glu Leu Thr Arg Ile
180 185 190
Ile Tyr Thr Pro Leu Met Gly Val Leu Glu Asp Ser Ser Ser Ile Ser
195 200 205
Ala Thr Arg Lys Glu Tyr Gly Asn Ile Ala Ala Ile Asn Gln Gln Asp
210 215 220
Gly Ala Thr Thr Ile Pro Pro Ala Leu Phe Ile Trp Leu Glu Lys Gln
225 230 235 240
Ile Val Tyr Pro Tyr Asn Asn Leu Ile Tyr Ser Tyr Gln Asn Phe Gln
245 250 255
Lys Thr Thr Phe Gly Lys Val Met Asn Gly Thr Ile Phe Gly Ser Asn
260 265 270
Ser Glu Lys His Leu Thr Asn Pro Ile Ser Ile Pro Ile Asp Ala Glu
275 280 285
Ser Tyr Asp Val Tyr Lys Val Asp Thr Ser Tyr Ser Ala Lys Met Glu
290 295 300
Ile Lys Ser Ser Pro Ile His Lys Leu Val Tyr Tyr Arg Ser Lys Glu
305 310 315 320
Gln Gln Glu Ser Ile Ile Ser Thr Asn Thr Ser Lys Gly Pro Ile Asp
325 330 335
Gln Val Ser Glu Ile Ala Asn Glu Gly Tyr Gln Asp Tyr Ser His Cys
340 345 350
Leu Ala His Met Ala Gly Trp Val Ser Ile Ala Tyr Gly Thr Gly Leu
355 360 365
Thr Glu Lys Pro Tyr Leu Val Pro Tyr Asn Leu Ala Leu Gly Trp Thr
370 375 380
Tyr Ala Asn Val Asp Pro Val Asn Ser Ile Ala Pro Asp Ala Ile Thr
385 390 395 400
Gln Ile Pro Ala Val Lys Gly Asp Lys Val Ile Gly Ile Pro Glu Glu
405 410 415
Glu Ala Ile Leu Gly Glu Val Thr Ala Ile Gln Gly Pro Gly Phe Thr
420 425 430
Gly Gly Asn Leu Val Gly Leu Phe Ala Gly Ala Glu Leu His Met Lys
435 440 445
Val Thr Asn Pro Val Ser Asn Val Ala Gly Tyr Gln Met Arg Ile Arg
450 455 460
Tyr Ala Asn Asn His Pro Thr Ile Leu Ala Val Ser Tyr Gln Gly Val
465 470 475 480
Glu Thr Ser Ser Gly Lys Phe Asp Val Pro Val Thr Tyr Ser Gly Asp
485 490 495
Phe Lys Thr Lys Leu Thr Tyr Asn Ala Phe Lys Phe Lys Glu Ala Ile
500 505 510
Ile Ile Pro Ser Pro Leu Arg Glu Glu Ile Ala Asp Ile Val Leu Arg
515 520 525
Asn Glu Gly Asp Ser Asn Leu Leu Ile Asp Lys Ile Glu Leu Ile Pro
530 535 540
Met Asp Phe Trp Arg His Glu Gln Cys Asn
545 550
<210> 32
<211> 1239
<212> PRT
<213> 苏云金芽孢杆菌
<400> 32
Met Asn Gln Gln His Asn Asn Glu Tyr Glu Ile Met Ser Thr Gly Asp
1 5 10 15
Met Gly Tyr Gln Pro Arg Tyr Pro Phe Ser Asn Ala Pro Gly Ala Glu
20 25 30
Leu Gln Gln Met His Tyr Lys Asp Trp Met Asp Met Cys Ala Asp Gly
35 40 45
Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val Gln Glu Gly Val Thr
50 55 60
Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser Val Pro Phe Pro Val
65 70 75 80
Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu Val Pro Tyr Trp Trp
85 90 95
Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser Ala Gln Val Thr Trp
100 105 110
Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val
115 120 125
Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr
130 135 140
Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln
145 150 155 160
Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe
165 170 175
Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe
180 185 190
Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu
195 200 205
Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln
210 215 220
Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly
225 230 235 240
Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val
245 250 255
Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr
260 265 270
Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val
275 280 285
Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro
290 295 300
Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val
305 310 315 320
Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala
325 330 335
Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu
340 345 350
Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly
355 360 365
Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu
370 375 380
Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala
385 390 395 400
Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr
405 410 415
Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe
420 425 430
Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro
435 440 445
Glu Ile Val Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser
450 455 460
Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn
465 470 475 480
Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser
485 490 495
Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe
500 505 510
Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp
515 520 525
Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly
530 535 540
Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu
545 550 555 560
Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu
565 570 575
Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala
580 585 590
Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp
595 600 605
Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr
610 615 620
Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser
625 630 635 640
Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile
645 650 655
Leu Asp Lys Ile Glu Phe Ile Pro Ile Glu Gly Ser Val Asp Glu Tyr
660 665 670
Gln Ala Asn Gln Asp Leu Glu Lys Ala Lys Lys Ala Val Asn Ala Leu
675 680 685
Phe Thr Gly Asp Ala Lys Ser Ala Leu Lys Leu Ser Ile Thr Gly Tyr
690 695 700
Ile Val Asp Gln Ala Ala Asn Phe Val Glu Cys Val Ser Asp Glu Phe
705 710 715 720
His Ala Gln Glu Lys Met Ile Leu Leu Asp Gln Val Lys Phe Ala Lys
725 730 735
Arg Leu Ser Gln Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser
740 745 750
Ser Asp Trp Ser Gly Glu Asn Gly Trp Arg Thr Ser Pro His Val His
755 760 765
Val Ala Ser Asn Asn Pro Ile Phe Lys Gly Arg Tyr Leu His Met Pro
770 775 780
Gly Ala Met Ser Pro Gln Phe Ser Asn Asn Thr Tyr Pro Thr Tyr Ala
785 790 795 800
Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Ser Tyr Thr Arg Tyr Leu
805 810 815
Val Arg Gly Leu Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu
820 825 830
Arg Tyr Gly Lys Asp Val His Val Glu Met Asp Val Pro Asn Asp Ile
835 840 845
Gln Tyr Thr Leu Pro Thr Asn Asp Cys Gly Gly Phe Asp Arg Cys Lys
850 855 860
Pro Val Ser Tyr Gln Thr Gly Thr Ser Ser Tyr Lys Ser Cys Gly Cys
865 870 875 880
Lys Asn Asn Asp Thr Tyr Gln Asn Gly Met His Leu Ser Lys Ser Cys
885 890 895
Gly Cys Lys Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr Gly
900 905 910
Cys Val Asp Gln Glu Glu Asn Leu Gly Leu Phe Phe Ala Leu Lys Ile
915 920 925
Ala Ser Glu Asn Gly Met Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu
930 935 940
Ala Gln Pro Leu Lys Gly Glu Ala Leu Ala Arg Val Lys Lys Arg Glu
945 950 955 960
Gln Lys Trp Lys Gln Glu Met Ala Gln Lys Leu Leu Arg Thr Glu Lys
965 970 975
Ala Val Gln Ala Ala Lys Asp Ala Leu Gln Thr Leu Phe Thr Asn Ala
980 985 990
Gln Tyr Asn Arg Leu Lys Phe Glu Thr Leu Phe Pro Gln Ile Val His
995 1000 1005
Ala Glu Lys Leu Val Gln Gln Ile Pro Tyr Ala Tyr His Pro Phe
1010 1015 1020
Leu Ser Gly Thr Leu Ser Thr Val Pro Gly Met Asn Phe Glu Ile
1025 1030 1035
Ile Gln Gln Leu Leu Ala Val Ile Gly Asn Ala Arg Thr Leu Tyr
1040 1045 1050
Glu Gln Arg Asn Leu Leu Arg Thr Gly Thr Phe Ser Ser Gly Thr
1055 1060 1065
Gly Ser Trp Lys Val Thr Glu Gly Val Lys Val Gln Pro Leu Gln
1070 1075 1080
Asp Thr Ser Val Leu Val Leu Ser Glu Trp Ser His Glu Ala Ser
1085 1090 1095
Gln Gln Leu His Met Asp Pro Asp Arg Gly Tyr Val Leu Arg Val
1100 1105 1110
Thr Ala Arg Lys Glu Gly Gly Gly Lys Gly Thr Val Thr Met Ser
1115 1120 1125
Asp Cys Ala Asp Tyr Thr Glu Thr Leu Thr Phe Thr Ser Cys Asp
1130 1135 1140
Tyr Asn Thr Tyr Gly Ser Gln Thr Met Thr Ser Gly Thr Leu Ser
1145 1150 1155
Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro Asp Thr Asp Arg
1160 1165 1170
Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe Gln Val Glu
1175 1180 1185
Ser Val Glu Leu Ile Cys Met Glu Gln Met Glu Asp Asp Leu Tyr
1190 1195 1200
Asn Met Ala Gly Asn Val Ala Glu Glu Met Gln Val Leu Gln Gln
1205 1210 1215
Ser Arg Ser Gly Ser His Thr Leu Asp Pro Leu Cys Asn Thr Arg
1220 1225 1230
Ile Gly Glu Phe Asp Cys
1235
<210> 33
<211> 1228
<212> PRT
<213> 苏云金芽孢杆菌
<400> 33
Met Ser Thr Gly Asp Met Gly Tyr Gln Pro Arg Tyr Pro Phe Ser Asn
1 5 10 15
Ala Pro Gly Ala Glu Leu Gln Gln Met His Tyr Lys Asp Trp Met Asp
20 25 30
Met Cys Ala Asp Gly Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val
35 40 45
Gln Glu Gly Val Thr Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser
50 55 60
Val Pro Phe Pro Val Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu
65 70 75 80
Val Pro Tyr Trp Trp Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser
85 90 95
Ala Gln Val Thr Trp Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser
100 105 110
Asn Thr Gln Ile Val Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp
115 120 125
Gln Gly Ile Gln Thr Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp
130 135 140
Trp Leu Gln Asp Gln Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu
145 150 155 160
Ala Phe Asp Asp Phe Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe
165 170 175
Arg Ala Gln Gly Phe Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala
180 185 190
Ala Asn Met His Leu Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val
195 200 205
Gly Trp Gly Phe Gln Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr
210 215 220
Asp Pro Phe Leu Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr
225 230 235 240
Thr Asp Tyr Cys Val Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr
245 250 255
Glu Asn Asn Arg Tyr Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp
260 265 270
Met Ile Ile Met Val Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp
275 280 285
Pro Lys Arg Tyr Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val
290 295 300
Tyr Thr Asp Leu Val Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile
305 310 315 320
Asp Ile Glu Arg Ala Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe
325 330 335
Thr Trp Leu Arg Glu Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile
340 345 350
Asn Phe Val Arg Gly Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser
355 360 365
Asp Arg Tyr Glu Glu Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr
370 375 380
Val Val Ile Pro Ala Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser
385 390 395 400
Thr Gln Val Asn Thr Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly
405 410 415
Trp Asn Phe Ser Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg
420 425 430
Thr Glu Tyr Ser Pro Glu Ile Val Met Gln Gly Leu Ser Cys His Gly
435 440 445
Pro Ser Val Ser Ser Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg
450 455 460
Ser Ile Thr Pro Asn Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr
465 470 475 480
Ser His Arg Phe Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr
485 490 495
Thr Leu Ile Tyr Phe Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala
500 505 510
Asn Asn Leu Ile Asp Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys
515 520 525
Gly Asp Tyr Leu Gly Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser
530 535 540
Thr Gly Gly Asp Leu Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr
545 550 555 560
Leu Gly Ile Lys Leu Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val
565 570 575
Arg Ile Arg Tyr Ala Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp
580 585 590
Gly Glu Asp Tyr Asp Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile
595 600 605
Thr Asn Leu Thr Tyr Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe
610 615 620
Ser Tyr Asn Ser Ser Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala
625 630 635 640
Thr Gly Ser Phe Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile Glu Gly
645 650 655
Ser Val Asp Glu Tyr Gln Ala Asn Gln Asp Leu Glu Lys Ala Lys Lys
660 665 670
Ala Val Asn Ala Leu Phe Thr Gly Asp Ala Lys Ser Ala Leu Lys Leu
675 680 685
Ser Ile Thr Gly Tyr Ile Val Asp Gln Ala Ala Asn Phe Val Glu Cys
690 695 700
Val Ser Asp Glu Phe His Ala Gln Glu Lys Met Ile Leu Leu Asp Gln
705 710 715 720
Val Lys Phe Ala Lys Arg Leu Ser Gln Ala Arg Asn Leu Leu Asn Tyr
725 730 735
Gly Asp Phe Glu Ser Ser Asp Trp Ser Gly Glu Asn Gly Trp Arg Thr
740 745 750
Ser Pro His Val His Val Ala Ser Asn Asn Pro Ile Phe Lys Gly Arg
755 760 765
Tyr Leu His Met Pro Gly Ala Met Ser Pro Gln Phe Ser Asn Asn Thr
770 775 780
Tyr Pro Thr Tyr Ala Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Ser
785 790 795 800
Tyr Thr Arg Tyr Leu Val Arg Gly Leu Val Gly Asn Ser Lys Asp Leu
805 810 815
Glu Leu Leu Val Glu Arg Tyr Gly Lys Asp Val His Val Glu Met Asp
820 825 830
Val Pro Asn Asp Ile Gln Tyr Thr Leu Pro Thr Asn Asp Cys Gly Gly
835 840 845
Phe Asp Arg Cys Lys Pro Val Ser Tyr Gln Thr Gly Thr Ser Ser Tyr
850 855 860
Lys Ser Cys Gly Cys Lys Asn Asn Asp Thr Tyr Gln Asn Gly Met His
865 870 875 880
Leu Ser Lys Ser Cys Gly Cys Lys Lys Asp Pro His Val Phe Thr Tyr
885 890 895
His Ile Asp Thr Gly Cys Val Asp Gln Glu Glu Asn Leu Gly Leu Phe
900 905 910
Phe Ala Leu Lys Ile Ala Ser Glu Asn Gly Met Ala Asn Ile Asp Asn
915 920 925
Leu Glu Ile Ile Glu Ala Gln Pro Leu Lys Gly Glu Ala Leu Ala Arg
930 935 940
Val Lys Lys Arg Glu Gln Lys Trp Lys Gln Glu Met Ala Gln Lys Leu
945 950 955 960
Leu Arg Thr Glu Lys Ala Val Gln Ala Ala Lys Asp Ala Leu Gln Thr
965 970 975
Leu Phe Thr Asn Ala Gln Tyr Asn Arg Leu Lys Phe Glu Thr Leu Phe
980 985 990
Pro Gln Ile Val His Ala Glu Lys Leu Val Gln Gln Ile Pro Tyr Ala
995 1000 1005
Tyr His Pro Phe Leu Ser Gly Thr Leu Ser Thr Val Pro Gly Met
1010 1015 1020
Asn Phe Glu Ile Ile Gln Gln Leu Leu Ala Val Ile Gly Asn Ala
1025 1030 1035
Arg Thr Leu Tyr Glu Gln Arg Asn Leu Leu Arg Thr Gly Thr Phe
1040 1045 1050
Ser Ser Gly Thr Gly Ser Trp Lys Val Thr Glu Gly Val Lys Val
1055 1060 1065
Gln Pro Leu Gln Asp Thr Ser Val Leu Val Leu Ser Glu Trp Ser
1070 1075 1080
His Glu Ala Ser Gln Gln Leu His Met Asp Pro Asp Arg Gly Tyr
1085 1090 1095
Val Leu Arg Val Thr Ala Arg Lys Glu Gly Gly Gly Lys Gly Thr
1100 1105 1110
Val Thr Met Ser Asp Cys Ala Asp Tyr Thr Glu Thr Leu Thr Phe
1115 1120 1125
Thr Ser Cys Asp Tyr Asn Thr Tyr Gly Ser Gln Thr Met Thr Ser
1130 1135 1140
Gly Thr Leu Ser Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro
1145 1150 1155
Asp Thr Asp Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr
1160 1165 1170
Phe Gln Val Glu Ser Val Glu Leu Ile Cys Met Glu Gln Met Glu
1175 1180 1185
Asp Asp Leu Tyr Asn Met Ala Gly Asn Val Ala Glu Glu Met Gln
1190 1195 1200
Val Leu Gln Gln Ser Arg Ser Gly Ser His Thr Leu Asp Pro Leu
1205 1210 1215
Cys Asn Thr Arg Ile Gly Glu Phe Asp Cys
1220 1225
<210> 34
<211> 1223
<212> PRT
<213> 苏云金芽孢杆菌
<400> 34
Met Gly Tyr Gln Pro Arg Tyr Pro Phe Ser Asn Ala Pro Gly Ala Glu
1 5 10 15
Leu Gln Gln Met His Tyr Lys Asp Trp Met Asp Met Cys Ala Asp Gly
20 25 30
Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val Gln Glu Gly Val Thr
35 40 45
Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser Val Pro Phe Pro Val
50 55 60
Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu Val Pro Tyr Trp Trp
65 70 75 80
Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser Ala Gln Val Thr Trp
85 90 95
Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val
100 105 110
Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr
115 120 125
Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln
130 135 140
Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe
145 150 155 160
Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe
165 170 175
Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu
180 185 190
Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln
195 200 205
Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly
210 215 220
Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val
225 230 235 240
Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr
245 250 255
Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val
260 265 270
Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro
275 280 285
Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val
290 295 300
Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala
305 310 315 320
Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu
325 330 335
Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly
340 345 350
Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu
355 360 365
Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala
370 375 380
Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr
385 390 395 400
Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe
405 410 415
Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro
420 425 430
Glu Ile Val Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser
435 440 445
Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn
450 455 460
Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser
465 470 475 480
Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe
485 490 495
Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp
500 505 510
Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly
515 520 525
Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu
530 535 540
Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu
545 550 555 560
Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala
565 570 575
Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp
580 585 590
Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr
595 600 605
Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser
610 615 620
Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile
625 630 635 640
Leu Asp Lys Ile Glu Phe Ile Pro Ile Glu Gly Ser Val Asp Glu Tyr
645 650 655
Gln Ala Asn Gln Asp Leu Glu Lys Ala Lys Lys Ala Val Asn Ala Leu
660 665 670
Phe Thr Gly Asp Ala Lys Ser Ala Leu Lys Leu Ser Ile Thr Gly Tyr
675 680 685
Ile Val Asp Gln Ala Ala Asn Phe Val Glu Cys Val Ser Asp Glu Phe
690 695 700
His Ala Gln Glu Lys Met Ile Leu Leu Asp Gln Val Lys Phe Ala Lys
705 710 715 720
Arg Leu Ser Gln Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser
725 730 735
Ser Asp Trp Ser Gly Glu Asn Gly Trp Arg Thr Ser Pro His Val His
740 745 750
Val Ala Ser Asn Asn Pro Ile Phe Lys Gly Arg Tyr Leu His Met Pro
755 760 765
Gly Ala Met Ser Pro Gln Phe Ser Asn Asn Thr Tyr Pro Thr Tyr Ala
770 775 780
Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Ser Tyr Thr Arg Tyr Leu
785 790 795 800
Val Arg Gly Leu Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu
805 810 815
Arg Tyr Gly Lys Asp Val His Val Glu Met Asp Val Pro Asn Asp Ile
820 825 830
Gln Tyr Thr Leu Pro Thr Asn Asp Cys Gly Gly Phe Asp Arg Cys Lys
835 840 845
Pro Val Ser Tyr Gln Thr Gly Thr Ser Ser Tyr Lys Ser Cys Gly Cys
850 855 860
Lys Asn Asn Asp Thr Tyr Gln Asn Gly Met His Leu Ser Lys Ser Cys
865 870 875 880
Gly Cys Lys Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr Gly
885 890 895
Cys Val Asp Gln Glu Glu Asn Leu Gly Leu Phe Phe Ala Leu Lys Ile
900 905 910
Ala Ser Glu Asn Gly Met Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu
915 920 925
Ala Gln Pro Leu Lys Gly Glu Ala Leu Ala Arg Val Lys Lys Arg Glu
930 935 940
Gln Lys Trp Lys Gln Glu Met Ala Gln Lys Leu Leu Arg Thr Glu Lys
945 950 955 960
Ala Val Gln Ala Ala Lys Asp Ala Leu Gln Thr Leu Phe Thr Asn Ala
965 970 975
Gln Tyr Asn Arg Leu Lys Phe Glu Thr Leu Phe Pro Gln Ile Val His
980 985 990
Ala Glu Lys Leu Val Gln Gln Ile Pro Tyr Ala Tyr His Pro Phe Leu
995 1000 1005
Ser Gly Thr Leu Ser Thr Val Pro Gly Met Asn Phe Glu Ile Ile
1010 1015 1020
Gln Gln Leu Leu Ala Val Ile Gly Asn Ala Arg Thr Leu Tyr Glu
1025 1030 1035
Gln Arg Asn Leu Leu Arg Thr Gly Thr Phe Ser Ser Gly Thr Gly
1040 1045 1050
Ser Trp Lys Val Thr Glu Gly Val Lys Val Gln Pro Leu Gln Asp
1055 1060 1065
Thr Ser Val Leu Val Leu Ser Glu Trp Ser His Glu Ala Ser Gln
1070 1075 1080
Gln Leu His Met Asp Pro Asp Arg Gly Tyr Val Leu Arg Val Thr
1085 1090 1095
Ala Arg Lys Glu Gly Gly Gly Lys Gly Thr Val Thr Met Ser Asp
1100 1105 1110
Cys Ala Asp Tyr Thr Glu Thr Leu Thr Phe Thr Ser Cys Asp Tyr
1115 1120 1125
Asn Thr Tyr Gly Ser Gln Thr Met Thr Ser Gly Thr Leu Ser Gly
1130 1135 1140
Phe Val Thr Lys Thr Leu Glu Ile Phe Pro Asp Thr Asp Arg Ile
1145 1150 1155
Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe Gln Val Glu Ser
1160 1165 1170
Val Glu Leu Ile Cys Met Glu Gln Met Glu Asp Asp Leu Tyr Asn
1175 1180 1185
Met Ala Gly Asn Val Ala Glu Glu Met Gln Val Leu Gln Gln Ser
1190 1195 1200
Arg Ser Gly Ser His Thr Leu Asp Pro Leu Cys Asn Thr Arg Ile
1205 1210 1215
Gly Glu Phe Asp Cys
1220
<210> 35
<211> 1204
<212> PRT
<213> 苏云金芽孢杆菌
<400> 35
Met His Tyr Lys Asp Trp Met Asp Met Cys Ala Asp Gly Glu Ser Gly
1 5 10 15
Lys Thr Phe Ala Asp Leu Thr Val Gln Glu Gly Val Thr Ile Ala Val
20 25 30
Ser Ile Ala Ala Ala Ile Leu Ser Val Pro Phe Pro Val Thr Ala Ala
35 40 45
Gly Leu Ser Ile Ile Ser Leu Leu Val Pro Tyr Trp Trp Pro Glu Thr
50 55 60
Ala Val Thr Pro Gly Thr Pro Ser Ala Gln Val Thr Trp Glu Lys Phe
65 70 75 80
Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val Ala Ser Lys
85 90 95
Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr Leu Gly Arg
100 105 110
Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln Asn Asn Glu
115 120 125
Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe Glu Asp Tyr
130 135 140
Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe Glu Ile Pro
145 150 155 160
Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu Leu Leu Leu
165 170 175
Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln Gln Tyr Glu
180 185 190
Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly Asn Pro Gly
195 200 205
Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val Lys Trp Tyr
210 215 220
Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr Asn Trp Asp
225 230 235 240
Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val Leu Asp Ile
245 250 255
Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro Leu Pro Thr
260 265 270
Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val Gly Phe Ser
275 280 285
Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala Glu Gln Ala
290 295 300
Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu Leu Ser Phe
305 310 315 320
Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly Arg Gln Ile
325 330 335
Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu Thr Lys Gly
340 345 350
Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala Pro Asp Val
355 360 365
Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr Tyr Gln Ile
370 375 380
Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe Thr Gln Ser
385 390 395 400
Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro Glu Ile Val
405 410 415
Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser Cys Asn Leu
420 425 430
Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn Tyr Ser Ser
435 440 445
Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser Tyr Leu Gly
450 455 460
Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe Ser Tyr Gly
465 470 475 480
Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp Pro Lys Lys
485 490 495
Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly Arg Asn Ala
500 505 510
Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Gln Leu
515 520 525
Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu Thr Lys Pro
530 535 540
Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala Ser Asn Thr
545 550 555 560
Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp Ser Val Ile
565 570 575
Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr Asn Lys Phe
580 585 590
Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser Ser Glu Glu
595 600 605
Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile Leu Asp Lys
610 615 620
Ile Glu Phe Ile Pro Ile Glu Gly Ser Val Asp Glu Tyr Gln Ala Asn
625 630 635 640
Gln Asp Leu Glu Lys Ala Lys Lys Ala Val Asn Ala Leu Phe Thr Gly
645 650 655
Asp Ala Lys Ser Ala Leu Lys Leu Ser Ile Thr Gly Tyr Ile Val Asp
660 665 670
Gln Ala Ala Asn Phe Val Glu Cys Val Ser Asp Glu Phe His Ala Gln
675 680 685
Glu Lys Met Ile Leu Leu Asp Gln Val Lys Phe Ala Lys Arg Leu Ser
690 695 700
Gln Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser Ser Asp Trp
705 710 715 720
Ser Gly Glu Asn Gly Trp Arg Thr Ser Pro His Val His Val Ala Ser
725 730 735
Asn Asn Pro Ile Phe Lys Gly Arg Tyr Leu His Met Pro Gly Ala Met
740 745 750
Ser Pro Gln Phe Ser Asn Asn Thr Tyr Pro Thr Tyr Ala Tyr Gln Lys
755 760 765
Val Asp Glu Ser Lys Leu Lys Ser Tyr Thr Arg Tyr Leu Val Arg Gly
770 775 780
Leu Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly
785 790 795 800
Lys Asp Val His Val Glu Met Asp Val Pro Asn Asp Ile Gln Tyr Thr
805 810 815
Leu Pro Thr Asn Asp Cys Gly Gly Phe Asp Arg Cys Lys Pro Val Ser
820 825 830
Tyr Gln Thr Gly Thr Ser Ser Tyr Lys Ser Cys Gly Cys Lys Asn Asn
835 840 845
Asp Thr Tyr Gln Asn Gly Met His Leu Ser Lys Ser Cys Gly Cys Lys
850 855 860
Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr Gly Cys Val Asp
865 870 875 880
Gln Glu Glu Asn Leu Gly Leu Phe Phe Ala Leu Lys Ile Ala Ser Glu
885 890 895
Asn Gly Met Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu Ala Gln Pro
900 905 910
Leu Lys Gly Glu Ala Leu Ala Arg Val Lys Lys Arg Glu Gln Lys Trp
915 920 925
Lys Gln Glu Met Ala Gln Lys Leu Leu Arg Thr Glu Lys Ala Val Gln
930 935 940
Ala Ala Lys Asp Ala Leu Gln Thr Leu Phe Thr Asn Ala Gln Tyr Asn
945 950 955 960
Arg Leu Lys Phe Glu Thr Leu Phe Pro Gln Ile Val His Ala Glu Lys
965 970 975
Leu Val Gln Gln Ile Pro Tyr Ala Tyr His Pro Phe Leu Ser Gly Thr
980 985 990
Leu Ser Thr Val Pro Gly Met Asn Phe Glu Ile Ile Gln Gln Leu Leu
995 1000 1005
Ala Val Ile Gly Asn Ala Arg Thr Leu Tyr Glu Gln Arg Asn Leu
1010 1015 1020
Leu Arg Thr Gly Thr Phe Ser Ser Gly Thr Gly Ser Trp Lys Val
1025 1030 1035
Thr Glu Gly Val Lys Val Gln Pro Leu Gln Asp Thr Ser Val Leu
1040 1045 1050
Val Leu Ser Glu Trp Ser His Glu Ala Ser Gln Gln Leu His Met
1055 1060 1065
Asp Pro Asp Arg Gly Tyr Val Leu Arg Val Thr Ala Arg Lys Glu
1070 1075 1080
Gly Gly Gly Lys Gly Thr Val Thr Met Ser Asp Cys Ala Asp Tyr
1085 1090 1095
Thr Glu Thr Leu Thr Phe Thr Ser Cys Asp Tyr Asn Thr Tyr Gly
1100 1105 1110
Ser Gln Thr Met Thr Ser Gly Thr Leu Ser Gly Phe Val Thr Lys
1115 1120 1125
Thr Leu Glu Ile Phe Pro Asp Thr Asp Arg Ile Arg Ile Asp Ile
1130 1135 1140
Gly Glu Thr Glu Gly Thr Phe Gln Val Glu Ser Val Glu Leu Ile
1145 1150 1155
Cys Met Glu Gln Met Glu Asp Asp Leu Tyr Asn Met Ala Gly Asn
1160 1165 1170
Val Ala Glu Glu Met Gln Val Leu Gln Gln Ser Arg Ser Gly Ser
1175 1180 1185
His Thr Leu Asp Pro Leu Cys Asn Thr Arg Ile Gly Glu Phe Asp
1190 1195 1200
Cys
<210> 36
<211> 1198
<212> PRT
<213> 苏云金芽孢杆菌
<400> 36
Met Asp Met Cys Ala Asp Gly Glu Ser Gly Lys Thr Phe Ala Asp Leu
1 5 10 15
Thr Val Gln Glu Gly Val Thr Ile Ala Val Ser Ile Ala Ala Ala Ile
20 25 30
Leu Ser Val Pro Phe Pro Val Thr Ala Ala Gly Leu Ser Ile Ile Ser
35 40 45
Leu Leu Val Pro Tyr Trp Trp Pro Glu Thr Ala Val Thr Pro Gly Thr
50 55 60
Pro Ser Ala Gln Val Thr Trp Glu Lys Phe Met Ser Ala Ala Glu Asn
65 70 75 80
Leu Ser Asn Thr Gln Ile Val Ala Ser Lys Arg Ser Asp Ala Ile Ala
85 90 95
Arg Trp Gln Gly Ile Gln Thr Leu Gly Arg Asp Tyr Phe Gln Ala Gln
100 105 110
Cys Asp Trp Leu Gln Asp Gln Asn Asn Glu Leu Lys Lys Ser Lys Leu
115 120 125
Arg Glu Ala Phe Asp Asp Phe Glu Asp Tyr Leu Lys Val Ser Met Pro
130 135 140
Phe Phe Arg Ala Gln Gly Phe Glu Ile Pro Met Leu Ala Met Tyr Ala
145 150 155 160
Gln Ala Ala Asn Met His Leu Leu Leu Leu Arg Glu Val Val Gln Asn
165 170 175
Gly Val Gly Trp Gly Phe Gln Gln Tyr Glu Val Asp Arg Tyr Tyr Ser
180 185 190
Asn Thr Asp Pro Phe Leu Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu
195 200 205
Gly Tyr Thr Asp Tyr Cys Val Lys Trp Tyr Asn Ala Gly Leu Arg Gln
210 215 220
Gln Tyr Glu Asn Asn Arg Tyr Asn Trp Asp Ala Phe Asn Asp Phe Arg
225 230 235 240
Arg Asp Met Ile Ile Met Val Leu Asp Ile Val Ser Leu Trp Pro Thr
245 250 255
Tyr Asp Pro Lys Arg Tyr Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg
260 265 270
Thr Val Tyr Thr Asp Leu Val Gly Phe Ser Gly Asn Ser Glu Tyr Leu
275 280 285
Gln Ile Asp Ile Glu Arg Ala Glu Gln Ala Leu Val Gln Lys Pro Gly
290 295 300
Leu Phe Thr Trp Leu Arg Glu Leu Ser Phe Glu Leu Gly Pro Leu Ser
305 310 315 320
Arg Ile Asn Phe Val Arg Gly Arg Gln Ile Val Phe Asn Tyr Thr Gly
325 330 335
Ser Ser Asp Arg Tyr Glu Glu Thr Lys Gly Asn Leu Gly Glu Thr Arg
340 345 350
Glu Thr Val Val Ile Pro Ala Pro Asp Val Gly Asp Asp Ile Trp Arg
355 360 365
Ile Ser Thr Gln Val Asn Thr Tyr Gln Ile Pro Asn Ala Thr Phe Val
370 375 380
Arg Gly Trp Asn Phe Ser Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala
385 390 395 400
Trp Arg Thr Glu Tyr Ser Pro Glu Ile Val Met Gln Gly Leu Ser Cys
405 410 415
His Gly Pro Ser Val Ser Ser Cys Asn Leu Cys Ile Ser Asn Ser Pro
420 425 430
Cys Arg Ser Ile Thr Pro Asn Tyr Ser Ser Pro Cys Asp Asp Lys Leu
435 440 445
Val Tyr Ser His Arg Phe Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp
450 455 460
Leu Thr Thr Leu Ile Tyr Phe Ser Tyr Gly Trp Thr His Val Ser Ala
465 470 475 480
Asp Ala Asn Asn Leu Ile Asp Pro Lys Lys Ile Thr Gln Ile Pro Ala
485 490 495
Val Lys Gly Asp Tyr Leu Gly Arg Asn Ala Arg Val Ile Lys Gly Pro
500 505 510
Gly Ser Thr Gly Gly Asp Leu Val Gln Leu Ser Asp Gly Thr Glu Arg
515 520 525
Gly Thr Leu Gly Ile Lys Leu Thr Lys Pro Pro Gly Ser His Ser Tyr
530 535 540
Arg Val Arg Ile Arg Tyr Ala Ser Asn Thr Arg Thr Gln Leu Glu Ile
545 550 555 560
Ile Trp Gly Glu Asp Tyr Asp Ser Val Ile Val Pro Ala Thr Thr Thr
565 570 575
Asp Ile Thr Asn Leu Thr Tyr Asn Lys Phe Gly Tyr Phe Glu Ile Arg
580 585 590
Val Phe Ser Tyr Asn Ser Ser Ser Glu Glu Glu Asp Leu Ile Arg Val
595 600 605
Asp Ala Thr Gly Ser Phe Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile
610 615 620
Glu Gly Ser Val Asp Glu Tyr Gln Ala Asn Gln Asp Leu Glu Lys Ala
625 630 635 640
Lys Lys Ala Val Asn Ala Leu Phe Thr Gly Asp Ala Lys Ser Ala Leu
645 650 655
Lys Leu Ser Ile Thr Gly Tyr Ile Val Asp Gln Ala Ala Asn Phe Val
660 665 670
Glu Cys Val Ser Asp Glu Phe His Ala Gln Glu Lys Met Ile Leu Leu
675 680 685
Asp Gln Val Lys Phe Ala Lys Arg Leu Ser Gln Ala Arg Asn Leu Leu
690 695 700
Asn Tyr Gly Asp Phe Glu Ser Ser Asp Trp Ser Gly Glu Asn Gly Trp
705 710 715 720
Arg Thr Ser Pro His Val His Val Ala Ser Asn Asn Pro Ile Phe Lys
725 730 735
Gly Arg Tyr Leu His Met Pro Gly Ala Met Ser Pro Gln Phe Ser Asn
740 745 750
Asn Thr Tyr Pro Thr Tyr Ala Tyr Gln Lys Val Asp Glu Ser Lys Leu
755 760 765
Lys Ser Tyr Thr Arg Tyr Leu Val Arg Gly Leu Val Gly Asn Ser Lys
770 775 780
Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly Lys Asp Val His Val Glu
785 790 795 800
Met Asp Val Pro Asn Asp Ile Gln Tyr Thr Leu Pro Thr Asn Asp Cys
805 810 815
Gly Gly Phe Asp Arg Cys Lys Pro Val Ser Tyr Gln Thr Gly Thr Ser
820 825 830
Ser Tyr Lys Ser Cys Gly Cys Lys Asn Asn Asp Thr Tyr Gln Asn Gly
835 840 845
Met His Leu Ser Lys Ser Cys Gly Cys Lys Lys Asp Pro His Val Phe
850 855 860
Thr Tyr His Ile Asp Thr Gly Cys Val Asp Gln Glu Glu Asn Leu Gly
865 870 875 880
Leu Phe Phe Ala Leu Lys Ile Ala Ser Glu Asn Gly Met Ala Asn Ile
885 890 895
Asp Asn Leu Glu Ile Ile Glu Ala Gln Pro Leu Lys Gly Glu Ala Leu
900 905 910
Ala Arg Val Lys Lys Arg Glu Gln Lys Trp Lys Gln Glu Met Ala Gln
915 920 925
Lys Leu Leu Arg Thr Glu Lys Ala Val Gln Ala Ala Lys Asp Ala Leu
930 935 940
Gln Thr Leu Phe Thr Asn Ala Gln Tyr Asn Arg Leu Lys Phe Glu Thr
945 950 955 960
Leu Phe Pro Gln Ile Val His Ala Glu Lys Leu Val Gln Gln Ile Pro
965 970 975
Tyr Ala Tyr His Pro Phe Leu Ser Gly Thr Leu Ser Thr Val Pro Gly
980 985 990
Met Asn Phe Glu Ile Ile Gln Gln Leu Leu Ala Val Ile Gly Asn Ala
995 1000 1005
Arg Thr Leu Tyr Glu Gln Arg Asn Leu Leu Arg Thr Gly Thr Phe
1010 1015 1020
Ser Ser Gly Thr Gly Ser Trp Lys Val Thr Glu Gly Val Lys Val
1025 1030 1035
Gln Pro Leu Gln Asp Thr Ser Val Leu Val Leu Ser Glu Trp Ser
1040 1045 1050
His Glu Ala Ser Gln Gln Leu His Met Asp Pro Asp Arg Gly Tyr
1055 1060 1065
Val Leu Arg Val Thr Ala Arg Lys Glu Gly Gly Gly Lys Gly Thr
1070 1075 1080
Val Thr Met Ser Asp Cys Ala Asp Tyr Thr Glu Thr Leu Thr Phe
1085 1090 1095
Thr Ser Cys Asp Tyr Asn Thr Tyr Gly Ser Gln Thr Met Thr Ser
1100 1105 1110
Gly Thr Leu Ser Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro
1115 1120 1125
Asp Thr Asp Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr
1130 1135 1140
Phe Gln Val Glu Ser Val Glu Leu Ile Cys Met Glu Gln Met Glu
1145 1150 1155
Asp Asp Leu Tyr Asn Met Ala Gly Asn Val Ala Glu Glu Met Gln
1160 1165 1170
Val Leu Gln Gln Ser Arg Ser Gly Ser His Thr Leu Asp Pro Leu
1175 1180 1185
Cys Asn Thr Arg Ile Gly Glu Phe Asp Cys
1190 1195
<210> 37
<211> 1196
<212> PRT
<213> 苏云金芽孢杆菌
<400> 37
Met Cys Ala Asp Gly Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val
1 5 10 15
Gln Glu Gly Val Thr Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser
20 25 30
Val Pro Phe Pro Val Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu
35 40 45
Val Pro Tyr Trp Trp Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser
50 55 60
Ala Gln Val Thr Trp Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser
65 70 75 80
Asn Thr Gln Ile Val Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp
85 90 95
Gln Gly Ile Gln Thr Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp
100 105 110
Trp Leu Gln Asp Gln Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu
115 120 125
Ala Phe Asp Asp Phe Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe
130 135 140
Arg Ala Gln Gly Phe Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala
145 150 155 160
Ala Asn Met His Leu Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val
165 170 175
Gly Trp Gly Phe Gln Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr
180 185 190
Asp Pro Phe Leu Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr
195 200 205
Thr Asp Tyr Cys Val Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr
210 215 220
Glu Asn Asn Arg Tyr Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp
225 230 235 240
Met Ile Ile Met Val Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp
245 250 255
Pro Lys Arg Tyr Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val
260 265 270
Tyr Thr Asp Leu Val Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile
275 280 285
Asp Ile Glu Arg Ala Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe
290 295 300
Thr Trp Leu Arg Glu Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile
305 310 315 320
Asn Phe Val Arg Gly Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser
325 330 335
Asp Arg Tyr Glu Glu Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr
340 345 350
Val Val Ile Pro Ala Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser
355 360 365
Thr Gln Val Asn Thr Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly
370 375 380
Trp Asn Phe Ser Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg
385 390 395 400
Thr Glu Tyr Ser Pro Glu Ile Val Met Gln Gly Leu Ser Cys His Gly
405 410 415
Pro Ser Val Ser Ser Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg
420 425 430
Ser Ile Thr Pro Asn Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr
435 440 445
Ser His Arg Phe Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr
450 455 460
Thr Leu Ile Tyr Phe Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala
465 470 475 480
Asn Asn Leu Ile Asp Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys
485 490 495
Gly Asp Tyr Leu Gly Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser
500 505 510
Thr Gly Gly Asp Leu Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr
515 520 525
Leu Gly Ile Lys Leu Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val
530 535 540
Arg Ile Arg Tyr Ala Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp
545 550 555 560
Gly Glu Asp Tyr Asp Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile
565 570 575
Thr Asn Leu Thr Tyr Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe
580 585 590
Ser Tyr Asn Ser Ser Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala
595 600 605
Thr Gly Ser Phe Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile Glu Gly
610 615 620
Ser Val Asp Glu Tyr Gln Ala Asn Gln Asp Leu Glu Lys Ala Lys Lys
625 630 635 640
Ala Val Asn Ala Leu Phe Thr Gly Asp Ala Lys Ser Ala Leu Lys Leu
645 650 655
Ser Ile Thr Gly Tyr Ile Val Asp Gln Ala Ala Asn Phe Val Glu Cys
660 665 670
Val Ser Asp Glu Phe His Ala Gln Glu Lys Met Ile Leu Leu Asp Gln
675 680 685
Val Lys Phe Ala Lys Arg Leu Ser Gln Ala Arg Asn Leu Leu Asn Tyr
690 695 700
Gly Asp Phe Glu Ser Ser Asp Trp Ser Gly Glu Asn Gly Trp Arg Thr
705 710 715 720
Ser Pro His Val His Val Ala Ser Asn Asn Pro Ile Phe Lys Gly Arg
725 730 735
Tyr Leu His Met Pro Gly Ala Met Ser Pro Gln Phe Ser Asn Asn Thr
740 745 750
Tyr Pro Thr Tyr Ala Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Ser
755 760 765
Tyr Thr Arg Tyr Leu Val Arg Gly Leu Val Gly Asn Ser Lys Asp Leu
770 775 780
Glu Leu Leu Val Glu Arg Tyr Gly Lys Asp Val His Val Glu Met Asp
785 790 795 800
Val Pro Asn Asp Ile Gln Tyr Thr Leu Pro Thr Asn Asp Cys Gly Gly
805 810 815
Phe Asp Arg Cys Lys Pro Val Ser Tyr Gln Thr Gly Thr Ser Ser Tyr
820 825 830
Lys Ser Cys Gly Cys Lys Asn Asn Asp Thr Tyr Gln Asn Gly Met His
835 840 845
Leu Ser Lys Ser Cys Gly Cys Lys Lys Asp Pro His Val Phe Thr Tyr
850 855 860
His Ile Asp Thr Gly Cys Val Asp Gln Glu Glu Asn Leu Gly Leu Phe
865 870 875 880
Phe Ala Leu Lys Ile Ala Ser Glu Asn Gly Met Ala Asn Ile Asp Asn
885 890 895
Leu Glu Ile Ile Glu Ala Gln Pro Leu Lys Gly Glu Ala Leu Ala Arg
900 905 910
Val Lys Lys Arg Glu Gln Lys Trp Lys Gln Glu Met Ala Gln Lys Leu
915 920 925
Leu Arg Thr Glu Lys Ala Val Gln Ala Ala Lys Asp Ala Leu Gln Thr
930 935 940
Leu Phe Thr Asn Ala Gln Tyr Asn Arg Leu Lys Phe Glu Thr Leu Phe
945 950 955 960
Pro Gln Ile Val His Ala Glu Lys Leu Val Gln Gln Ile Pro Tyr Ala
965 970 975
Tyr His Pro Phe Leu Ser Gly Thr Leu Ser Thr Val Pro Gly Met Asn
980 985 990
Phe Glu Ile Ile Gln Gln Leu Leu Ala Val Ile Gly Asn Ala Arg Thr
995 1000 1005
Leu Tyr Glu Gln Arg Asn Leu Leu Arg Thr Gly Thr Phe Ser Ser
1010 1015 1020
Gly Thr Gly Ser Trp Lys Val Thr Glu Gly Val Lys Val Gln Pro
1025 1030 1035
Leu Gln Asp Thr Ser Val Leu Val Leu Ser Glu Trp Ser His Glu
1040 1045 1050
Ala Ser Gln Gln Leu His Met Asp Pro Asp Arg Gly Tyr Val Leu
1055 1060 1065
Arg Val Thr Ala Arg Lys Glu Gly Gly Gly Lys Gly Thr Val Thr
1070 1075 1080
Met Ser Asp Cys Ala Asp Tyr Thr Glu Thr Leu Thr Phe Thr Ser
1085 1090 1095
Cys Asp Tyr Asn Thr Tyr Gly Ser Gln Thr Met Thr Ser Gly Thr
1100 1105 1110
Leu Ser Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro Asp Thr
1115 1120 1125
Asp Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe Gln
1130 1135 1140
Val Glu Ser Val Glu Leu Ile Cys Met Glu Gln Met Glu Asp Asp
1145 1150 1155
Leu Tyr Asn Met Ala Gly Asn Val Ala Glu Glu Met Gln Val Leu
1160 1165 1170
Gln Gln Ser Arg Ser Gly Ser His Thr Leu Asp Pro Leu Cys Asn
1175 1180 1185
Thr Arg Ile Gly Glu Phe Asp Cys
1190 1195
<210> 38
<211> 1124
<212> PRT
<213> 苏云金芽孢杆菌
<400> 38
Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val Ala Ser Lys
1 5 10 15
Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr Leu Gly Arg
20 25 30
Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln Asn Asn Glu
35 40 45
Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe Glu Asp Tyr
50 55 60
Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe Glu Ile Pro
65 70 75 80
Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu Leu Leu Leu
85 90 95
Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln Gln Tyr Glu
100 105 110
Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly Asn Pro Gly
115 120 125
Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val Lys Trp Tyr
130 135 140
Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr Asn Trp Asp
145 150 155 160
Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val Leu Asp Ile
165 170 175
Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro Leu Pro Thr
180 185 190
Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val Gly Phe Ser
195 200 205
Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala Glu Gln Ala
210 215 220
Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu Leu Ser Phe
225 230 235 240
Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly Arg Gln Ile
245 250 255
Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu Thr Lys Gly
260 265 270
Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala Pro Asp Val
275 280 285
Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr Tyr Gln Ile
290 295 300
Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe Thr Gln Ser
305 310 315 320
Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro Glu Ile Val
325 330 335
Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser Cys Asn Leu
340 345 350
Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn Tyr Ser Ser
355 360 365
Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser Tyr Leu Gly
370 375 380
Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe Ser Tyr Gly
385 390 395 400
Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp Pro Lys Lys
405 410 415
Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly Arg Asn Ala
420 425 430
Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Gln Leu
435 440 445
Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu Thr Lys Pro
450 455 460
Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala Ser Asn Thr
465 470 475 480
Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp Ser Val Ile
485 490 495
Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr Asn Lys Phe
500 505 510
Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser Ser Glu Glu
515 520 525
Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile Leu Asp Lys
530 535 540
Ile Glu Phe Ile Pro Ile Glu Gly Ser Val Asp Glu Tyr Gln Ala Asn
545 550 555 560
Gln Asp Leu Glu Lys Ala Lys Lys Ala Val Asn Ala Leu Phe Thr Gly
565 570 575
Asp Ala Lys Ser Ala Leu Lys Leu Ser Ile Thr Gly Tyr Ile Val Asp
580 585 590
Gln Ala Ala Asn Phe Val Glu Cys Val Ser Asp Glu Phe His Ala Gln
595 600 605
Glu Lys Met Ile Leu Leu Asp Gln Val Lys Phe Ala Lys Arg Leu Ser
610 615 620
Gln Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser Ser Asp Trp
625 630 635 640
Ser Gly Glu Asn Gly Trp Arg Thr Ser Pro His Val His Val Ala Ser
645 650 655
Asn Asn Pro Ile Phe Lys Gly Arg Tyr Leu His Met Pro Gly Ala Met
660 665 670
Ser Pro Gln Phe Ser Asn Asn Thr Tyr Pro Thr Tyr Ala Tyr Gln Lys
675 680 685
Val Asp Glu Ser Lys Leu Lys Ser Tyr Thr Arg Tyr Leu Val Arg Gly
690 695 700
Leu Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly
705 710 715 720
Lys Asp Val His Val Glu Met Asp Val Pro Asn Asp Ile Gln Tyr Thr
725 730 735
Leu Pro Thr Asn Asp Cys Gly Gly Phe Asp Arg Cys Lys Pro Val Ser
740 745 750
Tyr Gln Thr Gly Thr Ser Ser Tyr Lys Ser Cys Gly Cys Lys Asn Asn
755 760 765
Asp Thr Tyr Gln Asn Gly Met His Leu Ser Lys Ser Cys Gly Cys Lys
770 775 780
Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr Gly Cys Val Asp
785 790 795 800
Gln Glu Glu Asn Leu Gly Leu Phe Phe Ala Leu Lys Ile Ala Ser Glu
805 810 815
Asn Gly Met Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu Ala Gln Pro
820 825 830
Leu Lys Gly Glu Ala Leu Ala Arg Val Lys Lys Arg Glu Gln Lys Trp
835 840 845
Lys Gln Glu Met Ala Gln Lys Leu Leu Arg Thr Glu Lys Ala Val Gln
850 855 860
Ala Ala Lys Asp Ala Leu Gln Thr Leu Phe Thr Asn Ala Gln Tyr Asn
865 870 875 880
Arg Leu Lys Phe Glu Thr Leu Phe Pro Gln Ile Val His Ala Glu Lys
885 890 895
Leu Val Gln Gln Ile Pro Tyr Ala Tyr His Pro Phe Leu Ser Gly Thr
900 905 910
Leu Ser Thr Val Pro Gly Met Asn Phe Glu Ile Ile Gln Gln Leu Leu
915 920 925
Ala Val Ile Gly Asn Ala Arg Thr Leu Tyr Glu Gln Arg Asn Leu Leu
930 935 940
Arg Thr Gly Thr Phe Ser Ser Gly Thr Gly Ser Trp Lys Val Thr Glu
945 950 955 960
Gly Val Lys Val Gln Pro Leu Gln Asp Thr Ser Val Leu Val Leu Ser
965 970 975
Glu Trp Ser His Glu Ala Ser Gln Gln Leu His Met Asp Pro Asp Arg
980 985 990
Gly Tyr Val Leu Arg Val Thr Ala Arg Lys Glu Gly Gly Gly Lys Gly
995 1000 1005
Thr Val Thr Met Ser Asp Cys Ala Asp Tyr Thr Glu Thr Leu Thr
1010 1015 1020
Phe Thr Ser Cys Asp Tyr Asn Thr Tyr Gly Ser Gln Thr Met Thr
1025 1030 1035
Ser Gly Thr Leu Ser Gly Phe Val Thr Lys Thr Leu Glu Ile Phe
1040 1045 1050
Pro Asp Thr Asp Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly
1055 1060 1065
Thr Phe Gln Val Glu Ser Val Glu Leu Ile Cys Met Glu Gln Met
1070 1075 1080
Glu Asp Asp Leu Tyr Asn Met Ala Gly Asn Val Ala Glu Glu Met
1085 1090 1095
Gln Val Leu Gln Gln Ser Arg Ser Gly Ser His Thr Leu Asp Pro
1100 1105 1110
Leu Cys Asn Thr Arg Ile Gly Glu Phe Asp Cys
1115 1120
<210> 39
<211> 665
<212> PRT
<213> 苏云金芽孢杆菌
<400> 39
Met Asn Gln Gln His Asn Asn Glu Tyr Glu Ile Met Ser Thr Gly Asp
1 5 10 15
Met Gly Tyr Gln Pro Arg Tyr Pro Phe Ser Asn Ala Pro Gly Ala Glu
20 25 30
Leu Gln Gln Met His Tyr Lys Asp Trp Met Asp Met Cys Ala Asp Gly
35 40 45
Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val Gln Glu Gly Val Thr
50 55 60
Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser Val Pro Phe Pro Val
65 70 75 80
Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu Val Pro Tyr Trp Trp
85 90 95
Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser Ala Gln Val Thr Trp
100 105 110
Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val
115 120 125
Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr
130 135 140
Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln
145 150 155 160
Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe
165 170 175
Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe
180 185 190
Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu
195 200 205
Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln
210 215 220
Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly
225 230 235 240
Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val
245 250 255
Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr
260 265 270
Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val
275 280 285
Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro
290 295 300
Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val
305 310 315 320
Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala
325 330 335
Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu
340 345 350
Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly
355 360 365
Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu
370 375 380
Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala
385 390 395 400
Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr
405 410 415
Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe
420 425 430
Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro
435 440 445
Glu Ile Val Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser
450 455 460
Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn
465 470 475 480
Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser
485 490 495
Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe
500 505 510
Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp
515 520 525
Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly
530 535 540
Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu
545 550 555 560
Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu
565 570 575
Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala
580 585 590
Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp
595 600 605
Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr
610 615 620
Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser
625 630 635 640
Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile
645 650 655
Leu Asp Lys Ile Glu Phe Ile Pro Ile
660 665
<210> 40
<211> 654
<212> PRT
<213> 苏云金芽孢杆菌
<400> 40
Met Ser Thr Gly Asp Met Gly Tyr Gln Pro Arg Tyr Pro Phe Ser Asn
1 5 10 15
Ala Pro Gly Ala Glu Leu Gln Gln Met His Tyr Lys Asp Trp Met Asp
20 25 30
Met Cys Ala Asp Gly Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val
35 40 45
Gln Glu Gly Val Thr Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser
50 55 60
Val Pro Phe Pro Val Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu
65 70 75 80
Val Pro Tyr Trp Trp Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser
85 90 95
Ala Gln Val Thr Trp Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser
100 105 110
Asn Thr Gln Ile Val Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp
115 120 125
Gln Gly Ile Gln Thr Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp
130 135 140
Trp Leu Gln Asp Gln Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu
145 150 155 160
Ala Phe Asp Asp Phe Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe
165 170 175
Arg Ala Gln Gly Phe Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala
180 185 190
Ala Asn Met His Leu Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val
195 200 205
Gly Trp Gly Phe Gln Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr
210 215 220
Asp Pro Phe Leu Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr
225 230 235 240
Thr Asp Tyr Cys Val Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr
245 250 255
Glu Asn Asn Arg Tyr Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp
260 265 270
Met Ile Ile Met Val Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp
275 280 285
Pro Lys Arg Tyr Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val
290 295 300
Tyr Thr Asp Leu Val Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile
305 310 315 320
Asp Ile Glu Arg Ala Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe
325 330 335
Thr Trp Leu Arg Glu Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile
340 345 350
Asn Phe Val Arg Gly Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser
355 360 365
Asp Arg Tyr Glu Glu Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr
370 375 380
Val Val Ile Pro Ala Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser
385 390 395 400
Thr Gln Val Asn Thr Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly
405 410 415
Trp Asn Phe Ser Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg
420 425 430
Thr Glu Tyr Ser Pro Glu Ile Val Met Gln Gly Leu Ser Cys His Gly
435 440 445
Pro Ser Val Ser Ser Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg
450 455 460
Ser Ile Thr Pro Asn Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr
465 470 475 480
Ser His Arg Phe Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr
485 490 495
Thr Leu Ile Tyr Phe Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala
500 505 510
Asn Asn Leu Ile Asp Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys
515 520 525
Gly Asp Tyr Leu Gly Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser
530 535 540
Thr Gly Gly Asp Leu Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr
545 550 555 560
Leu Gly Ile Lys Leu Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val
565 570 575
Arg Ile Arg Tyr Ala Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp
580 585 590
Gly Glu Asp Tyr Asp Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile
595 600 605
Thr Asn Leu Thr Tyr Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe
610 615 620
Ser Tyr Asn Ser Ser Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala
625 630 635 640
Thr Gly Ser Phe Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile
645 650
<210> 41
<211> 649
<212> PRT
<213> 苏云金芽孢杆菌
<400> 41
Met Gly Tyr Gln Pro Arg Tyr Pro Phe Ser Asn Ala Pro Gly Ala Glu
1 5 10 15
Leu Gln Gln Met His Tyr Lys Asp Trp Met Asp Met Cys Ala Asp Gly
20 25 30
Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val Gln Glu Gly Val Thr
35 40 45
Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser Val Pro Phe Pro Val
50 55 60
Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu Val Pro Tyr Trp Trp
65 70 75 80
Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser Ala Gln Val Thr Trp
85 90 95
Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val
100 105 110
Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr
115 120 125
Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln
130 135 140
Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe
145 150 155 160
Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe
165 170 175
Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu
180 185 190
Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln
195 200 205
Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly
210 215 220
Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val
225 230 235 240
Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr
245 250 255
Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val
260 265 270
Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro
275 280 285
Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val
290 295 300
Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala
305 310 315 320
Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu
325 330 335
Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly
340 345 350
Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu
355 360 365
Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala
370 375 380
Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr
385 390 395 400
Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe
405 410 415
Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro
420 425 430
Glu Ile Val Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser
435 440 445
Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn
450 455 460
Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser
465 470 475 480
Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe
485 490 495
Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp
500 505 510
Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly
515 520 525
Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu
530 535 540
Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu
545 550 555 560
Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala
565 570 575
Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp
580 585 590
Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr
595 600 605
Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser
610 615 620
Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile
625 630 635 640
Leu Asp Lys Ile Glu Phe Ile Pro Ile
645
<210> 42
<211> 630
<212> PRT
<213> 苏云金芽孢杆菌
<400> 42
Met His Tyr Lys Asp Trp Met Asp Met Cys Ala Asp Gly Glu Ser Gly
1 5 10 15
Lys Thr Phe Ala Asp Leu Thr Val Gln Glu Gly Val Thr Ile Ala Val
20 25 30
Ser Ile Ala Ala Ala Ile Leu Ser Val Pro Phe Pro Val Thr Ala Ala
35 40 45
Gly Leu Ser Ile Ile Ser Leu Leu Val Pro Tyr Trp Trp Pro Glu Thr
50 55 60
Ala Val Thr Pro Gly Thr Pro Ser Ala Gln Val Thr Trp Glu Lys Phe
65 70 75 80
Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val Ala Ser Lys
85 90 95
Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr Leu Gly Arg
100 105 110
Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln Asn Asn Glu
115 120 125
Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe Glu Asp Tyr
130 135 140
Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe Glu Ile Pro
145 150 155 160
Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu Leu Leu Leu
165 170 175
Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln Gln Tyr Glu
180 185 190
Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly Asn Pro Gly
195 200 205
Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val Lys Trp Tyr
210 215 220
Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr Asn Trp Asp
225 230 235 240
Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val Leu Asp Ile
245 250 255
Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro Leu Pro Thr
260 265 270
Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val Gly Phe Ser
275 280 285
Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala Glu Gln Ala
290 295 300
Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu Leu Ser Phe
305 310 315 320
Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly Arg Gln Ile
325 330 335
Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu Thr Lys Gly
340 345 350
Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala Pro Asp Val
355 360 365
Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr Tyr Gln Ile
370 375 380
Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe Thr Gln Ser
385 390 395 400
Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro Glu Ile Val
405 410 415
Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser Cys Asn Leu
420 425 430
Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn Tyr Ser Ser
435 440 445
Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser Tyr Leu Gly
450 455 460
Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe Ser Tyr Gly
465 470 475 480
Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp Pro Lys Lys
485 490 495
Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly Arg Asn Ala
500 505 510
Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Gln Leu
515 520 525
Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu Thr Lys Pro
530 535 540
Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala Ser Asn Thr
545 550 555 560
Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp Ser Val Ile
565 570 575
Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr Asn Lys Phe
580 585 590
Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser Ser Glu Glu
595 600 605
Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile Leu Asp Lys
610 615 620
Ile Glu Phe Ile Pro Ile
625 630
<210> 43
<211> 624
<212> PRT
<213> 苏云金芽孢杆菌
<400> 43
Met Asp Met Cys Ala Asp Gly Glu Ser Gly Lys Thr Phe Ala Asp Leu
1 5 10 15
Thr Val Gln Glu Gly Val Thr Ile Ala Val Ser Ile Ala Ala Ala Ile
20 25 30
Leu Ser Val Pro Phe Pro Val Thr Ala Ala Gly Leu Ser Ile Ile Ser
35 40 45
Leu Leu Val Pro Tyr Trp Trp Pro Glu Thr Ala Val Thr Pro Gly Thr
50 55 60
Pro Ser Ala Gln Val Thr Trp Glu Lys Phe Met Ser Ala Ala Glu Asn
65 70 75 80
Leu Ser Asn Thr Gln Ile Val Ala Ser Lys Arg Ser Asp Ala Ile Ala
85 90 95
Arg Trp Gln Gly Ile Gln Thr Leu Gly Arg Asp Tyr Phe Gln Ala Gln
100 105 110
Cys Asp Trp Leu Gln Asp Gln Asn Asn Glu Leu Lys Lys Ser Lys Leu
115 120 125
Arg Glu Ala Phe Asp Asp Phe Glu Asp Tyr Leu Lys Val Ser Met Pro
130 135 140
Phe Phe Arg Ala Gln Gly Phe Glu Ile Pro Met Leu Ala Met Tyr Ala
145 150 155 160
Gln Ala Ala Asn Met His Leu Leu Leu Leu Arg Glu Val Val Gln Asn
165 170 175
Gly Val Gly Trp Gly Phe Gln Gln Tyr Glu Val Asp Arg Tyr Tyr Ser
180 185 190
Asn Thr Asp Pro Phe Leu Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu
195 200 205
Gly Tyr Thr Asp Tyr Cys Val Lys Trp Tyr Asn Ala Gly Leu Arg Gln
210 215 220
Gln Tyr Glu Asn Asn Arg Tyr Asn Trp Asp Ala Phe Asn Asp Phe Arg
225 230 235 240
Arg Asp Met Ile Ile Met Val Leu Asp Ile Val Ser Leu Trp Pro Thr
245 250 255
Tyr Asp Pro Lys Arg Tyr Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg
260 265 270
Thr Val Tyr Thr Asp Leu Val Gly Phe Ser Gly Asn Ser Glu Tyr Leu
275 280 285
Gln Ile Asp Ile Glu Arg Ala Glu Gln Ala Leu Val Gln Lys Pro Gly
290 295 300
Leu Phe Thr Trp Leu Arg Glu Leu Ser Phe Glu Leu Gly Pro Leu Ser
305 310 315 320
Arg Ile Asn Phe Val Arg Gly Arg Gln Ile Val Phe Asn Tyr Thr Gly
325 330 335
Ser Ser Asp Arg Tyr Glu Glu Thr Lys Gly Asn Leu Gly Glu Thr Arg
340 345 350
Glu Thr Val Val Ile Pro Ala Pro Asp Val Gly Asp Asp Ile Trp Arg
355 360 365
Ile Ser Thr Gln Val Asn Thr Tyr Gln Ile Pro Asn Ala Thr Phe Val
370 375 380
Arg Gly Trp Asn Phe Ser Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala
385 390 395 400
Trp Arg Thr Glu Tyr Ser Pro Glu Ile Val Met Gln Gly Leu Ser Cys
405 410 415
His Gly Pro Ser Val Ser Ser Cys Asn Leu Cys Ile Ser Asn Ser Pro
420 425 430
Cys Arg Ser Ile Thr Pro Asn Tyr Ser Ser Pro Cys Asp Asp Lys Leu
435 440 445
Val Tyr Ser His Arg Phe Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp
450 455 460
Leu Thr Thr Leu Ile Tyr Phe Ser Tyr Gly Trp Thr His Val Ser Ala
465 470 475 480
Asp Ala Asn Asn Leu Ile Asp Pro Lys Lys Ile Thr Gln Ile Pro Ala
485 490 495
Val Lys Gly Asp Tyr Leu Gly Arg Asn Ala Arg Val Ile Lys Gly Pro
500 505 510
Gly Ser Thr Gly Gly Asp Leu Val Gln Leu Ser Asp Gly Thr Glu Arg
515 520 525
Gly Thr Leu Gly Ile Lys Leu Thr Lys Pro Pro Gly Ser His Ser Tyr
530 535 540
Arg Val Arg Ile Arg Tyr Ala Ser Asn Thr Arg Thr Gln Leu Glu Ile
545 550 555 560
Ile Trp Gly Glu Asp Tyr Asp Ser Val Ile Val Pro Ala Thr Thr Thr
565 570 575
Asp Ile Thr Asn Leu Thr Tyr Asn Lys Phe Gly Tyr Phe Glu Ile Arg
580 585 590
Val Phe Ser Tyr Asn Ser Ser Ser Glu Glu Glu Asp Leu Ile Arg Val
595 600 605
Asp Ala Thr Gly Ser Phe Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile
610 615 620
<210> 44
<211> 622
<212> PRT
<213> 苏云金芽孢杆菌
<400> 44
Met Cys Ala Asp Gly Glu Ser Gly Lys Thr Phe Ala Asp Leu Thr Val
1 5 10 15
Gln Glu Gly Val Thr Ile Ala Val Ser Ile Ala Ala Ala Ile Leu Ser
20 25 30
Val Pro Phe Pro Val Thr Ala Ala Gly Leu Ser Ile Ile Ser Leu Leu
35 40 45
Val Pro Tyr Trp Trp Pro Glu Thr Ala Val Thr Pro Gly Thr Pro Ser
50 55 60
Ala Gln Val Thr Trp Glu Lys Phe Met Ser Ala Ala Glu Asn Leu Ser
65 70 75 80
Asn Thr Gln Ile Val Ala Ser Lys Arg Ser Asp Ala Ile Ala Arg Trp
85 90 95
Gln Gly Ile Gln Thr Leu Gly Arg Asp Tyr Phe Gln Ala Gln Cys Asp
100 105 110
Trp Leu Gln Asp Gln Asn Asn Glu Leu Lys Lys Ser Lys Leu Arg Glu
115 120 125
Ala Phe Asp Asp Phe Glu Asp Tyr Leu Lys Val Ser Met Pro Phe Phe
130 135 140
Arg Ala Gln Gly Phe Glu Ile Pro Met Leu Ala Met Tyr Ala Gln Ala
145 150 155 160
Ala Asn Met His Leu Leu Leu Leu Arg Glu Val Val Gln Asn Gly Val
165 170 175
Gly Trp Gly Phe Gln Gln Tyr Glu Val Asp Arg Tyr Tyr Ser Asn Thr
180 185 190
Asp Pro Phe Leu Gly Asn Pro Gly Leu Leu Gln Leu Leu Glu Gly Tyr
195 200 205
Thr Asp Tyr Cys Val Lys Trp Tyr Asn Ala Gly Leu Arg Gln Gln Tyr
210 215 220
Glu Asn Asn Arg Tyr Asn Trp Asp Ala Phe Asn Asp Phe Arg Arg Asp
225 230 235 240
Met Ile Ile Met Val Leu Asp Ile Val Ser Leu Trp Pro Thr Tyr Asp
245 250 255
Pro Lys Arg Tyr Pro Leu Pro Thr Lys Ser Gln Leu Thr Arg Thr Val
260 265 270
Tyr Thr Asp Leu Val Gly Phe Ser Gly Asn Ser Glu Tyr Leu Gln Ile
275 280 285
Asp Ile Glu Arg Ala Glu Gln Ala Leu Val Gln Lys Pro Gly Leu Phe
290 295 300
Thr Trp Leu Arg Glu Leu Ser Phe Glu Leu Gly Pro Leu Ser Arg Ile
305 310 315 320
Asn Phe Val Arg Gly Arg Gln Ile Val Phe Asn Tyr Thr Gly Ser Ser
325 330 335
Asp Arg Tyr Glu Glu Thr Lys Gly Asn Leu Gly Glu Thr Arg Glu Thr
340 345 350
Val Val Ile Pro Ala Pro Asp Val Gly Asp Asp Ile Trp Arg Ile Ser
355 360 365
Thr Gln Val Asn Thr Tyr Gln Ile Pro Asn Ala Thr Phe Val Arg Gly
370 375 380
Trp Asn Phe Ser Phe Thr Gln Ser Leu Asp Gln Lys Ile Ala Trp Arg
385 390 395 400
Thr Glu Tyr Ser Pro Glu Ile Val Met Gln Gly Leu Ser Cys His Gly
405 410 415
Pro Ser Val Ser Ser Cys Asn Leu Cys Ile Ser Asn Ser Pro Cys Arg
420 425 430
Ser Ile Thr Pro Asn Tyr Ser Ser Pro Cys Asp Asp Lys Leu Val Tyr
435 440 445
Ser His Arg Phe Ser Tyr Leu Gly Ala Gly Leu Lys Ser Asp Leu Thr
450 455 460
Thr Leu Ile Tyr Phe Ser Tyr Gly Trp Thr His Val Ser Ala Asp Ala
465 470 475 480
Asn Asn Leu Ile Asp Pro Lys Lys Ile Thr Gln Ile Pro Ala Val Lys
485 490 495
Gly Asp Tyr Leu Gly Arg Asn Ala Arg Val Ile Lys Gly Pro Gly Ser
500 505 510
Thr Gly Gly Asp Leu Val Gln Leu Ser Asp Gly Thr Glu Arg Gly Thr
515 520 525
Leu Gly Ile Lys Leu Thr Lys Pro Pro Gly Ser His Ser Tyr Arg Val
530 535 540
Arg Ile Arg Tyr Ala Ser Asn Thr Arg Thr Gln Leu Glu Ile Ile Trp
545 550 555 560
Gly Glu Asp Tyr Asp Ser Val Ile Val Pro Ala Thr Thr Thr Asp Ile
565 570 575
Thr Asn Leu Thr Tyr Asn Lys Phe Gly Tyr Phe Glu Ile Arg Val Phe
580 585 590
Ser Tyr Asn Ser Ser Ser Glu Glu Glu Asp Leu Ile Arg Val Asp Ala
595 600 605
Thr Gly Ser Phe Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile
610 615 620
<210> 45
<211> 550
<212> PRT
<213> 苏云金芽孢杆菌
<400> 45
Met Ser Ala Ala Glu Asn Leu Ser Asn Thr Gln Ile Val Ala Ser Lys
1 5 10 15
Arg Ser Asp Ala Ile Ala Arg Trp Gln Gly Ile Gln Thr Leu Gly Arg
20 25 30
Asp Tyr Phe Gln Ala Gln Cys Asp Trp Leu Gln Asp Gln Asn Asn Glu
35 40 45
Leu Lys Lys Ser Lys Leu Arg Glu Ala Phe Asp Asp Phe Glu Asp Tyr
50 55 60
Leu Lys Val Ser Met Pro Phe Phe Arg Ala Gln Gly Phe Glu Ile Pro
65 70 75 80
Met Leu Ala Met Tyr Ala Gln Ala Ala Asn Met His Leu Leu Leu Leu
85 90 95
Arg Glu Val Val Gln Asn Gly Val Gly Trp Gly Phe Gln Gln Tyr Glu
100 105 110
Val Asp Arg Tyr Tyr Ser Asn Thr Asp Pro Phe Leu Gly Asn Pro Gly
115 120 125
Leu Leu Gln Leu Leu Glu Gly Tyr Thr Asp Tyr Cys Val Lys Trp Tyr
130 135 140
Asn Ala Gly Leu Arg Gln Gln Tyr Glu Asn Asn Arg Tyr Asn Trp Asp
145 150 155 160
Ala Phe Asn Asp Phe Arg Arg Asp Met Ile Ile Met Val Leu Asp Ile
165 170 175
Val Ser Leu Trp Pro Thr Tyr Asp Pro Lys Arg Tyr Pro Leu Pro Thr
180 185 190
Lys Ser Gln Leu Thr Arg Thr Val Tyr Thr Asp Leu Val Gly Phe Ser
195 200 205
Gly Asn Ser Glu Tyr Leu Gln Ile Asp Ile Glu Arg Ala Glu Gln Ala
210 215 220
Leu Val Gln Lys Pro Gly Leu Phe Thr Trp Leu Arg Glu Leu Ser Phe
225 230 235 240
Glu Leu Gly Pro Leu Ser Arg Ile Asn Phe Val Arg Gly Arg Gln Ile
245 250 255
Val Phe Asn Tyr Thr Gly Ser Ser Asp Arg Tyr Glu Glu Thr Lys Gly
260 265 270
Asn Leu Gly Glu Thr Arg Glu Thr Val Val Ile Pro Ala Pro Asp Val
275 280 285
Gly Asp Asp Ile Trp Arg Ile Ser Thr Gln Val Asn Thr Tyr Gln Ile
290 295 300
Pro Asn Ala Thr Phe Val Arg Gly Trp Asn Phe Ser Phe Thr Gln Ser
305 310 315 320
Leu Asp Gln Lys Ile Ala Trp Arg Thr Glu Tyr Ser Pro Glu Ile Val
325 330 335
Met Gln Gly Leu Ser Cys His Gly Pro Ser Val Ser Ser Cys Asn Leu
340 345 350
Cys Ile Ser Asn Ser Pro Cys Arg Ser Ile Thr Pro Asn Tyr Ser Ser
355 360 365
Pro Cys Asp Asp Lys Leu Val Tyr Ser His Arg Phe Ser Tyr Leu Gly
370 375 380
Ala Gly Leu Lys Ser Asp Leu Thr Thr Leu Ile Tyr Phe Ser Tyr Gly
385 390 395 400
Trp Thr His Val Ser Ala Asp Ala Asn Asn Leu Ile Asp Pro Lys Lys
405 410 415
Ile Thr Gln Ile Pro Ala Val Lys Gly Asp Tyr Leu Gly Arg Asn Ala
420 425 430
Arg Val Ile Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Gln Leu
435 440 445
Ser Asp Gly Thr Glu Arg Gly Thr Leu Gly Ile Lys Leu Thr Lys Pro
450 455 460
Pro Gly Ser His Ser Tyr Arg Val Arg Ile Arg Tyr Ala Ser Asn Thr
465 470 475 480
Arg Thr Gln Leu Glu Ile Ile Trp Gly Glu Asp Tyr Asp Ser Val Ile
485 490 495
Val Pro Ala Thr Thr Thr Asp Ile Thr Asn Leu Thr Tyr Asn Lys Phe
500 505 510
Gly Tyr Phe Glu Ile Arg Val Phe Ser Tyr Asn Ser Ser Ser Glu Glu
515 520 525
Glu Asp Leu Ile Arg Val Asp Ala Thr Gly Ser Phe Ile Leu Asp Lys
530 535 540
Ile Glu Phe Ile Pro Ile
545 550
<210> 46
<211> 1206
<212> PRT
<213> 苏云金芽孢杆菌
<400> 46
Met Asn Trp Leu Ser Lys Lys Cys Leu Ser Thr Leu Asn Val Asn Leu
1 5 10 15
Tyr Lys Ser Gln Phe Gln Gly Glu Tyr Met Asp Asn Asn Ser Glu Asn
20 25 30
Gln Cys Val Pro Tyr Asn Cys Leu Ser Asn Leu Glu Glu Ile Thr Leu
35 40 45
Asn Gly Glu Arg Leu Ser Thr Asn Ser Thr Pro Ile Asn Ile Ser Met
50 55 60
Ser Val Ser Lys Phe Leu Leu Thr Glu Leu Ile Pro Gly Leu Gly Phe
65 70 75 80
Val Phe Gly Leu Leu Asp Ala Ile Trp Gly Phe Ile Gly Pro Asp Gln
85 90 95
Trp Thr Glu Phe Ile Glu His Ile Glu Glu Leu Ile Gly Gln Arg Ile
100 105 110
Thr Val Val Val Arg Asn Thr Ala Ile Arg Glu Leu Glu Gly Met Ala
115 120 125
Arg Val Tyr Gln Thr Tyr Ala Thr Ala Phe Ala Ala Trp Glu Lys Asp
130 135 140
Pro Asn Asn Pro Glu Leu Arg Glu Ala Leu Arg Ala Gln Phe Thr Ala
145 150 155 160
Thr Glu Thr Tyr Ile Ser Gly Arg Ile Ser Val Leu Thr Ile Glu Asp
165 170 175
Tyr Gln Val Gln Leu Leu Ser Val Tyr Ala Gln Ala Thr Asn Leu His
180 185 190
Leu Ser Leu Leu Arg Asp Val Val Phe Trp Gly Gln Arg Trp Gly Phe
195 200 205
Ser Thr Thr Thr Leu Asn Asn Tyr Tyr Ser Asp Leu Thr Arg Glu Ile
210 215 220
Asn Glu Tyr Thr Asn Tyr Ala Val His Trp Tyr Asn Val Gly Leu Glu
225 230 235 240
Gln Leu Gln Gly Pro Ser Phe Gln Glu Trp Val Ala Tyr Asn Arg Tyr
245 250 255
Arg Arg Glu Leu Thr Leu Thr Val Leu Asp Ile Val Thr Leu Phe His
260 265 270
Asn Tyr Asp Ile Arg Leu Tyr Pro Ile Pro Thr Ile Ser Gln Leu Thr
275 280 285
Arg Glu Val Tyr Thr Asp Pro Ile Val Ser Gly Ile Gly Gln Pro Asn
290 295 300
Ser Trp Asp Phe Pro Thr Phe Ser Glu Ala Glu Asn Lys Ser Ile Arg
305 310 315 320
Thr Pro His Leu Met Asp Phe Leu Arg Asn Leu Thr Ile Phe Thr Asp
325 330 335
Ser Ala Arg Tyr Gly Ala Ile Tyr His Phe Trp Gly Gly His Gln Ile
340 345 350
Ser Ser Ser Leu Val Gly Gly Ser Asn Ile Thr Phe Pro Thr Tyr Gly
355 360 365
Ser Asn Val Ser Gln Gly Ser Pro Trp Ile Leu Val Thr Asn Gly Ile
370 375 380
Pro Ile Tyr Arg Thr Leu Ser Asn Pro Tyr Tyr Arg Phe Leu Phe Gln
385 390 395 400
Ser Val Gly Ser Ala Arg Leu Arg Cys Val Leu Gly Val Gln Phe His
405 410 415
Met Asp Asn Arg Ala Phe Thr Tyr Arg Glu Lys Gly Thr Val Asp Ser
420 425 430
Phe Asp Glu Leu Pro Pro Thr Asp Ala Ser Val Ser Pro Ser Glu Gly
435 440 445
Tyr Ser His Arg Leu Cys His Ala Thr Leu Phe Gln Val Arg Thr Gly
450 455 460
Gly Gly Gly Ala Val Ser Phe Ser Arg Thr Asp Gly Val Val Phe Ser
465 470 475 480
Trp Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Asn Val
485 490 495
Ile Thr Gln Ile Pro Ala Val Lys Gly Arg Ser Leu Phe Asn Gly Ala
500 505 510
Val Ile Lys Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Arg Leu Asn
515 520 525
Arg Asn Asn Gly Asn Ile Gln Asn Arg Gly His Leu Pro Ile Pro Ile
530 535 540
Gln Phe Ser Ser Arg Ser Thr Arg Tyr Arg Val Arg Leu Arg Tyr Ala
545 550 555 560
Ser Ala Thr Pro Ile Gln Val Asn Val His Trp Glu Asn Ser Thr Ile
565 570 575
Phe Ser Gly Ile Val Pro Ala Thr Ala Gln Ser Leu Asp Lys Leu Gln
580 585 590
Ser Asn Asp Phe Gly Tyr Phe Glu Ile Ala Asn Thr Ile Ser Ser Ser
595 600 605
Leu Asp Gly Ile Val Gly Ile Arg Asn Phe Ser Ala Asn Ala Asp Leu
610 615 620
Ile Ile Asp Arg Phe Glu Phe Ile Pro Val Asn Ala Thr Ser Glu Ala
625 630 635 640
Glu Tyr Asp Leu Glu Arg Ala Gln Glu Ala Val Asn Ala Leu Phe Thr
645 650 655
Ser Thr Asn Gln Arg Gly Leu Lys Ala Asn Val Thr Asp Tyr Tyr Ile
660 665 670
Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys Leu
675 680 685
Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys Gln Ala Lys Arg Ile
690 695 700
Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn Phe Arg Cys Ile Asn
705 710 715 720
Gly Glu Glu Asp Arg Gly Trp Arg Gly Ser Thr His Ile Thr Ile Gln
725 730 735
Gly Gly Asn Asp Val Phe Lys Arg Asn Phe Val Thr Leu Pro Gly Ala
740 745 750
Phe Asp Ala Cys Tyr Pro Thr Tyr Leu Tyr Gln Arg Ile Asp Glu Ser
755 760 765
Lys Leu Lys Ala Tyr Thr Arg Tyr Lys Leu Arg Gly Tyr Ile Glu Asp
770 775 780
Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His Glu
785 790 795 800
Thr Val Asn Val Pro Gly Thr Glu Ser Leu Trp Ser Leu Cys Thr Glu
805 810 815
Ser Pro Ile Gly Thr Cys Gly Glu Pro Asn Arg Cys Ala Pro Gln Ile
820 825 830
Glu Trp Asn Pro Asp Leu Asn Cys Ser Cys Lys Ala Gly Glu Lys Cys
835 840 845
Ala His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr
850 855 860
Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr
865 870 875 880
Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys
885 890 895
Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys
900 905 910
Trp Arg Asp Lys Arg Glu Lys Leu Gln Phe Glu Thr Lys Ile Val Tyr
915 920 925
Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asp Ser Gln Tyr
930 935 940
Asn Arg Leu Gln Ala Asp Thr Asn Ile Thr Met Ile His Ala Ala Asp
945 950 955 960
Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val
965 970 975
Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Leu Ile
980 985 990
Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly
995 1000 1005
Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val Lys Gly His Val
1010 1015 1020
Asp Val Gln Gln Ser His His Arg Ser Val Leu Val Leu Pro Glu
1025 1030 1035
Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val Cys Pro Gly Arg
1040 1045 1050
Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu
1055 1060 1065
Gly Cys Val Thr Ile His Glu Ile Glu Asn His Thr Glu Lys Leu
1070 1075 1080
Lys Phe Arg Asn Cys Glu Glu Glu Asp Ile Tyr Pro Thr Asn Thr
1085 1090 1095
Val Thr Cys His Asp Tyr Asn Val Asn Gln Gly Ala Glu Gly Cys
1100 1105 1110
Ala Asp Thr Cys Asn Ser Arg His Arg Gly Tyr Asp Glu Thr Tyr
1115 1120 1125
Gly Asn Asp Ser Ser Val Ser Ala Asp Tyr Met Pro Val Tyr Glu
1130 1135 1140
Glu Glu Val Tyr Thr Asp Gly Arg Arg Asp Asn Pro Cys Glu Met
1145 1150 1155
Glu Arg Gly Tyr Thr Pro Leu Pro Val Asp Tyr Val Thr Lys Glu
1160 1165 1170
Leu Glu Tyr Phe Pro Glu Thr Asn Thr Val Trp Ile Glu Ile Gly
1175 1180 1185
Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val Glu Leu Leu Leu
1190 1195 1200
Met Glu Glu
1205
<210> 47
<211> 1181
<212> PRT
<213> 苏云金芽孢杆菌
<400> 47
Met Asp Asn Asn Ser Glu Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser
1 5 10 15
Asn Leu Glu Glu Ile Thr Leu Asn Gly Glu Arg Leu Ser Thr Asn Ser
20 25 30
Thr Pro Ile Asn Ile Ser Met Ser Val Ser Lys Phe Leu Leu Thr Glu
35 40 45
Leu Ile Pro Gly Leu Gly Phe Val Phe Gly Leu Leu Asp Ala Ile Trp
50 55 60
Gly Phe Ile Gly Pro Asp Gln Trp Thr Glu Phe Ile Glu His Ile Glu
65 70 75 80
Glu Leu Ile Gly Gln Arg Ile Thr Val Val Val Arg Asn Thr Ala Ile
85 90 95
Arg Glu Leu Glu Gly Met Ala Arg Val Tyr Gln Thr Tyr Ala Thr Ala
100 105 110
Phe Ala Ala Trp Glu Lys Asp Pro Asn Asn Pro Glu Leu Arg Glu Ala
115 120 125
Leu Arg Ala Gln Phe Thr Ala Thr Glu Thr Tyr Ile Ser Gly Arg Ile
130 135 140
Ser Val Leu Thr Ile Glu Asp Tyr Gln Val Gln Leu Leu Ser Val Tyr
145 150 155 160
Ala Gln Ala Thr Asn Leu His Leu Ser Leu Leu Arg Asp Val Val Phe
165 170 175
Trp Gly Gln Arg Trp Gly Phe Ser Thr Thr Thr Leu Asn Asn Tyr Tyr
180 185 190
Ser Asp Leu Thr Arg Glu Ile Asn Glu Tyr Thr Asn Tyr Ala Val His
195 200 205
Trp Tyr Asn Val Gly Leu Glu Gln Leu Gln Gly Pro Ser Phe Gln Glu
210 215 220
Trp Val Ala Tyr Asn Arg Tyr Arg Arg Glu Leu Thr Leu Thr Val Leu
225 230 235 240
Asp Ile Val Thr Leu Phe His Asn Tyr Asp Ile Arg Leu Tyr Pro Ile
245 250 255
Pro Thr Ile Ser Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Ile Val
260 265 270
Ser Gly Ile Gly Gln Pro Asn Ser Trp Asp Phe Pro Thr Phe Ser Glu
275 280 285
Ala Glu Asn Lys Ser Ile Arg Thr Pro His Leu Met Asp Phe Leu Arg
290 295 300
Asn Leu Thr Ile Phe Thr Asp Ser Ala Arg Tyr Gly Ala Ile Tyr His
305 310 315 320
Phe Trp Gly Gly His Gln Ile Ser Ser Ser Leu Val Gly Gly Ser Asn
325 330 335
Ile Thr Phe Pro Thr Tyr Gly Ser Asn Val Ser Gln Gly Ser Pro Trp
340 345 350
Ile Leu Val Thr Asn Gly Ile Pro Ile Tyr Arg Thr Leu Ser Asn Pro
355 360 365
Tyr Tyr Arg Phe Leu Phe Gln Ser Val Gly Ser Ala Arg Leu Arg Cys
370 375 380
Val Leu Gly Val Gln Phe His Met Asp Asn Arg Ala Phe Thr Tyr Arg
385 390 395 400
Glu Lys Gly Thr Val Asp Ser Phe Asp Glu Leu Pro Pro Thr Asp Ala
405 410 415
Ser Val Ser Pro Ser Glu Gly Tyr Ser His Arg Leu Cys His Ala Thr
420 425 430
Leu Phe Gln Val Arg Thr Gly Gly Gly Gly Ala Val Ser Phe Ser Arg
435 440 445
Thr Asp Gly Val Val Phe Ser Trp Thr His Arg Ser Ala Thr Pro Thr
450 455 460
Asn Thr Ile Asp Pro Asn Val Ile Thr Gln Ile Pro Ala Val Lys Gly
465 470 475 480
Arg Ser Leu Phe Asn Gly Ala Val Ile Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Leu Val Arg Leu Asn Arg Asn Asn Gly Asn Ile Gln Asn Arg
500 505 510
Gly His Leu Pro Ile Pro Ile Gln Phe Ser Ser Arg Ser Thr Arg Tyr
515 520 525
Arg Val Arg Leu Arg Tyr Ala Ser Ala Thr Pro Ile Gln Val Asn Val
530 535 540
His Trp Glu Asn Ser Thr Ile Phe Ser Gly Ile Val Pro Ala Thr Ala
545 550 555 560
Gln Ser Leu Asp Lys Leu Gln Ser Asn Asp Phe Gly Tyr Phe Glu Ile
565 570 575
Ala Asn Thr Ile Ser Ser Ser Leu Asp Gly Ile Val Gly Ile Arg Asn
580 585 590
Phe Ser Ala Asn Ala Asp Leu Ile Ile Asp Arg Phe Glu Phe Ile Pro
595 600 605
Val Asn Ala Thr Ser Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Glu
610 615 620
Ala Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Arg Gly Leu Lys Ala
625 630 635 640
Asn Val Thr Asp Tyr Tyr Ile Asp Gln Val Ser Asn Leu Val Glu Cys
645 650 655
Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys
660 665 670
Val Lys Gln Ala Lys Arg Ile Ser Asp Glu Arg Asn Leu Leu Gln Asp
675 680 685
Ser Asn Phe Arg Cys Ile Asn Gly Glu Glu Asp Arg Gly Trp Arg Gly
690 695 700
Ser Thr His Ile Thr Ile Gln Gly Gly Asn Asp Val Phe Lys Arg Asn
705 710 715 720
Phe Val Thr Leu Pro Gly Ala Phe Asp Ala Cys Tyr Pro Thr Tyr Leu
725 730 735
Tyr Gln Arg Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Lys
740 745 750
Leu Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile
755 760 765
Arg Tyr Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Glu Ser
770 775 780
Leu Trp Ser Leu Cys Thr Glu Ser Pro Ile Gly Thr Cys Gly Glu Pro
785 790 795 800
Asn Arg Cys Ala Pro Gln Ile Glu Trp Asn Pro Asp Leu Asn Cys Ser
805 810 815
Cys Lys Ala Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu
820 825 830
Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp
835 840 845
Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn
850 855 860
Leu Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg
865 870 875 880
Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Gln
885 890 895
Phe Glu Thr Lys Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala
900 905 910
Leu Phe Val Asp Ser Gln Tyr Asn Arg Leu Gln Ala Asp Thr Asn Ile
915 920 925
Thr Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala
930 935 940
Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe
945 950 955 960
Glu Glu Leu Glu Gly Leu Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala
965 970 975
Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp
980 985 990
Asn Val Lys Gly His Val Asp Val Gln Gln Ser His His Arg Ser Val
995 1000 1005
Leu Val Leu Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg
1010 1015 1020
Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys
1025 1030 1035
Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn
1040 1045 1050
His Thr Glu Lys Leu Lys Phe Arg Asn Cys Glu Glu Glu Asp Ile
1055 1060 1065
Tyr Pro Thr Asn Thr Val Thr Cys His Asp Tyr Asn Val Asn Gln
1070 1075 1080
Gly Ala Glu Gly Cys Ala Asp Thr Cys Asn Ser Arg His Arg Gly
1085 1090 1095
Tyr Asp Glu Thr Tyr Gly Asn Asp Ser Ser Val Ser Ala Asp Tyr
1100 1105 1110
Met Pro Val Tyr Glu Glu Glu Val Tyr Thr Asp Gly Arg Arg Asp
1115 1120 1125
Asn Pro Cys Glu Met Glu Arg Gly Tyr Thr Pro Leu Pro Val Asp
1130 1135 1140
Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asn Thr Val
1145 1150 1155
Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser
1160 1165 1170
Val Glu Leu Leu Leu Met Glu Glu
1175 1180
<210> 48
<211> 1143
<212> PRT
<213> 苏云金芽孢杆菌
<400> 48
Met Ser Val Ser Lys Phe Leu Leu Thr Glu Leu Ile Pro Gly Leu Gly
1 5 10 15
Phe Val Phe Gly Leu Leu Asp Ala Ile Trp Gly Phe Ile Gly Pro Asp
20 25 30
Gln Trp Thr Glu Phe Ile Glu His Ile Glu Glu Leu Ile Gly Gln Arg
35 40 45
Ile Thr Val Val Val Arg Asn Thr Ala Ile Arg Glu Leu Glu Gly Met
50 55 60
Ala Arg Val Tyr Gln Thr Tyr Ala Thr Ala Phe Ala Ala Trp Glu Lys
65 70 75 80
Asp Pro Asn Asn Pro Glu Leu Arg Glu Ala Leu Arg Ala Gln Phe Thr
85 90 95
Ala Thr Glu Thr Tyr Ile Ser Gly Arg Ile Ser Val Leu Thr Ile Glu
100 105 110
Asp Tyr Gln Val Gln Leu Leu Ser Val Tyr Ala Gln Ala Thr Asn Leu
115 120 125
His Leu Ser Leu Leu Arg Asp Val Val Phe Trp Gly Gln Arg Trp Gly
130 135 140
Phe Ser Thr Thr Thr Leu Asn Asn Tyr Tyr Ser Asp Leu Thr Arg Glu
145 150 155 160
Ile Asn Glu Tyr Thr Asn Tyr Ala Val His Trp Tyr Asn Val Gly Leu
165 170 175
Glu Gln Leu Gln Gly Pro Ser Phe Gln Glu Trp Val Ala Tyr Asn Arg
180 185 190
Tyr Arg Arg Glu Leu Thr Leu Thr Val Leu Asp Ile Val Thr Leu Phe
195 200 205
His Asn Tyr Asp Ile Arg Leu Tyr Pro Ile Pro Thr Ile Ser Gln Leu
210 215 220
Thr Arg Glu Val Tyr Thr Asp Pro Ile Val Ser Gly Ile Gly Gln Pro
225 230 235 240
Asn Ser Trp Asp Phe Pro Thr Phe Ser Glu Ala Glu Asn Lys Ser Ile
245 250 255
Arg Thr Pro His Leu Met Asp Phe Leu Arg Asn Leu Thr Ile Phe Thr
260 265 270
Asp Ser Ala Arg Tyr Gly Ala Ile Tyr His Phe Trp Gly Gly His Gln
275 280 285
Ile Ser Ser Ser Leu Val Gly Gly Ser Asn Ile Thr Phe Pro Thr Tyr
290 295 300
Gly Ser Asn Val Ser Gln Gly Ser Pro Trp Ile Leu Val Thr Asn Gly
305 310 315 320
Ile Pro Ile Tyr Arg Thr Leu Ser Asn Pro Tyr Tyr Arg Phe Leu Phe
325 330 335
Gln Ser Val Gly Ser Ala Arg Leu Arg Cys Val Leu Gly Val Gln Phe
340 345 350
His Met Asp Asn Arg Ala Phe Thr Tyr Arg Glu Lys Gly Thr Val Asp
355 360 365
Ser Phe Asp Glu Leu Pro Pro Thr Asp Ala Ser Val Ser Pro Ser Glu
370 375 380
Gly Tyr Ser His Arg Leu Cys His Ala Thr Leu Phe Gln Val Arg Thr
385 390 395 400
Gly Gly Gly Gly Ala Val Ser Phe Ser Arg Thr Asp Gly Val Val Phe
405 410 415
Ser Trp Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Asn
420 425 430
Val Ile Thr Gln Ile Pro Ala Val Lys Gly Arg Ser Leu Phe Asn Gly
435 440 445
Ala Val Ile Lys Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Arg Leu
450 455 460
Asn Arg Asn Asn Gly Asn Ile Gln Asn Arg Gly His Leu Pro Ile Pro
465 470 475 480
Ile Gln Phe Ser Ser Arg Ser Thr Arg Tyr Arg Val Arg Leu Arg Tyr
485 490 495
Ala Ser Ala Thr Pro Ile Gln Val Asn Val His Trp Glu Asn Ser Thr
500 505 510
Ile Phe Ser Gly Ile Val Pro Ala Thr Ala Gln Ser Leu Asp Lys Leu
515 520 525
Gln Ser Asn Asp Phe Gly Tyr Phe Glu Ile Ala Asn Thr Ile Ser Ser
530 535 540
Ser Leu Asp Gly Ile Val Gly Ile Arg Asn Phe Ser Ala Asn Ala Asp
545 550 555 560
Leu Ile Ile Asp Arg Phe Glu Phe Ile Pro Val Asn Ala Thr Ser Glu
565 570 575
Ala Glu Tyr Asp Leu Glu Arg Ala Gln Glu Ala Val Asn Ala Leu Phe
580 585 590
Thr Ser Thr Asn Gln Arg Gly Leu Lys Ala Asn Val Thr Asp Tyr Tyr
595 600 605
Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys
610 615 620
Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys Gln Ala Lys Arg
625 630 635 640
Ile Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn Phe Arg Cys Ile
645 650 655
Asn Gly Glu Glu Asp Arg Gly Trp Arg Gly Ser Thr His Ile Thr Ile
660 665 670
Gln Gly Gly Asn Asp Val Phe Lys Arg Asn Phe Val Thr Leu Pro Gly
675 680 685
Ala Phe Asp Ala Cys Tyr Pro Thr Tyr Leu Tyr Gln Arg Ile Asp Glu
690 695 700
Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Lys Leu Arg Gly Tyr Ile Glu
705 710 715 720
Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His
725 730 735
Glu Thr Val Asn Val Pro Gly Thr Glu Ser Leu Trp Ser Leu Cys Thr
740 745 750
Glu Ser Pro Ile Gly Thr Cys Gly Glu Pro Asn Arg Cys Ala Pro Gln
755 760 765
Ile Glu Trp Asn Pro Asp Leu Asn Cys Ser Cys Lys Ala Gly Glu Lys
770 775 780
Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys
785 790 795 800
Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys
805 810 815
Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu
820 825 830
Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys
835 840 845
Lys Trp Arg Asp Lys Arg Glu Lys Leu Gln Phe Glu Thr Lys Ile Val
850 855 860
Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asp Ser Gln
865 870 875 880
Tyr Asn Arg Leu Gln Ala Asp Thr Asn Ile Thr Met Ile His Ala Ala
885 890 895
Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser
900 905 910
Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Leu
915 920 925
Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn
930 935 940
Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val Lys Gly His Val
945 950 955 960
Asp Val Gln Gln Ser His His Arg Ser Val Leu Val Leu Pro Glu Trp
965 970 975
Glu Ala Glu Val Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr
980 985 990
Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val
995 1000 1005
Thr Ile His Glu Ile Glu Asn His Thr Glu Lys Leu Lys Phe Arg
1010 1015 1020
Asn Cys Glu Glu Glu Asp Ile Tyr Pro Thr Asn Thr Val Thr Cys
1025 1030 1035
His Asp Tyr Asn Val Asn Gln Gly Ala Glu Gly Cys Ala Asp Thr
1040 1045 1050
Cys Asn Ser Arg His Arg Gly Tyr Asp Glu Thr Tyr Gly Asn Asp
1055 1060 1065
Ser Ser Val Ser Ala Asp Tyr Met Pro Val Tyr Glu Glu Glu Val
1070 1075 1080
Tyr Thr Asp Gly Arg Arg Asp Asn Pro Cys Glu Met Glu Arg Gly
1085 1090 1095
Tyr Thr Pro Leu Pro Val Asp Tyr Val Thr Lys Glu Leu Glu Tyr
1100 1105 1110
Phe Pro Glu Thr Asn Thr Val Trp Ile Glu Ile Gly Glu Thr Glu
1115 1120 1125
Gly Thr Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu
1130 1135 1140
<210> 49
<211> 634
<212> PRT
<213> 苏云金芽孢杆菌
<400> 49
Met Asn Trp Leu Ser Lys Lys Cys Leu Ser Thr Leu Asn Val Asn Leu
1 5 10 15
Tyr Lys Ser Gln Phe Gln Gly Glu Tyr Met Asp Asn Asn Ser Glu Asn
20 25 30
Gln Cys Val Pro Tyr Asn Cys Leu Ser Asn Leu Glu Glu Ile Thr Leu
35 40 45
Asn Gly Glu Arg Leu Ser Thr Asn Ser Thr Pro Ile Asn Ile Ser Met
50 55 60
Ser Val Ser Lys Phe Leu Leu Thr Glu Leu Ile Pro Gly Leu Gly Phe
65 70 75 80
Val Phe Gly Leu Leu Asp Ala Ile Trp Gly Phe Ile Gly Pro Asp Gln
85 90 95
Trp Thr Glu Phe Ile Glu His Ile Glu Glu Leu Ile Gly Gln Arg Ile
100 105 110
Thr Val Val Val Arg Asn Thr Ala Ile Arg Glu Leu Glu Gly Met Ala
115 120 125
Arg Val Tyr Gln Thr Tyr Ala Thr Ala Phe Ala Ala Trp Glu Lys Asp
130 135 140
Pro Asn Asn Pro Glu Leu Arg Glu Ala Leu Arg Ala Gln Phe Thr Ala
145 150 155 160
Thr Glu Thr Tyr Ile Ser Gly Arg Ile Ser Val Leu Thr Ile Glu Asp
165 170 175
Tyr Gln Val Gln Leu Leu Ser Val Tyr Ala Gln Ala Thr Asn Leu His
180 185 190
Leu Ser Leu Leu Arg Asp Val Val Phe Trp Gly Gln Arg Trp Gly Phe
195 200 205
Ser Thr Thr Thr Leu Asn Asn Tyr Tyr Ser Asp Leu Thr Arg Glu Ile
210 215 220
Asn Glu Tyr Thr Asn Tyr Ala Val His Trp Tyr Asn Val Gly Leu Glu
225 230 235 240
Gln Leu Gln Gly Pro Ser Phe Gln Glu Trp Val Ala Tyr Asn Arg Tyr
245 250 255
Arg Arg Glu Leu Thr Leu Thr Val Leu Asp Ile Val Thr Leu Phe His
260 265 270
Asn Tyr Asp Ile Arg Leu Tyr Pro Ile Pro Thr Ile Ser Gln Leu Thr
275 280 285
Arg Glu Val Tyr Thr Asp Pro Ile Val Ser Gly Ile Gly Gln Pro Asn
290 295 300
Ser Trp Asp Phe Pro Thr Phe Ser Glu Ala Glu Asn Lys Ser Ile Arg
305 310 315 320
Thr Pro His Leu Met Asp Phe Leu Arg Asn Leu Thr Ile Phe Thr Asp
325 330 335
Ser Ala Arg Tyr Gly Ala Ile Tyr His Phe Trp Gly Gly His Gln Ile
340 345 350
Ser Ser Ser Leu Val Gly Gly Ser Asn Ile Thr Phe Pro Thr Tyr Gly
355 360 365
Ser Asn Val Ser Gln Gly Ser Pro Trp Ile Leu Val Thr Asn Gly Ile
370 375 380
Pro Ile Tyr Arg Thr Leu Ser Asn Pro Tyr Tyr Arg Phe Leu Phe Gln
385 390 395 400
Ser Val Gly Ser Ala Arg Leu Arg Cys Val Leu Gly Val Gln Phe His
405 410 415
Met Asp Asn Arg Ala Phe Thr Tyr Arg Glu Lys Gly Thr Val Asp Ser
420 425 430
Phe Asp Glu Leu Pro Pro Thr Asp Ala Ser Val Ser Pro Ser Glu Gly
435 440 445
Tyr Ser His Arg Leu Cys His Ala Thr Leu Phe Gln Val Arg Thr Gly
450 455 460
Gly Gly Gly Ala Val Ser Phe Ser Arg Thr Asp Gly Val Val Phe Ser
465 470 475 480
Trp Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Asn Val
485 490 495
Ile Thr Gln Ile Pro Ala Val Lys Gly Arg Ser Leu Phe Asn Gly Ala
500 505 510
Val Ile Lys Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Arg Leu Asn
515 520 525
Arg Asn Asn Gly Asn Ile Gln Asn Arg Gly His Leu Pro Ile Pro Ile
530 535 540
Gln Phe Ser Ser Arg Ser Thr Arg Tyr Arg Val Arg Leu Arg Tyr Ala
545 550 555 560
Ser Ala Thr Pro Ile Gln Val Asn Val His Trp Glu Asn Ser Thr Ile
565 570 575
Phe Ser Gly Ile Val Pro Ala Thr Ala Gln Ser Leu Asp Lys Leu Gln
580 585 590
Ser Asn Asp Phe Gly Tyr Phe Glu Ile Ala Asn Thr Ile Ser Ser Ser
595 600 605
Leu Asp Gly Ile Val Gly Ile Arg Asn Phe Ser Ala Asn Ala Asp Leu
610 615 620
Ile Ile Asp Arg Phe Glu Phe Ile Pro Val
625 630
<210> 50
<211> 609
<212> PRT
<213> 苏云金芽孢杆菌
<400> 50
Met Asp Asn Asn Ser Glu Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser
1 5 10 15
Asn Leu Glu Glu Ile Thr Leu Asn Gly Glu Arg Leu Ser Thr Asn Ser
20 25 30
Thr Pro Ile Asn Ile Ser Met Ser Val Ser Lys Phe Leu Leu Thr Glu
35 40 45
Leu Ile Pro Gly Leu Gly Phe Val Phe Gly Leu Leu Asp Ala Ile Trp
50 55 60
Gly Phe Ile Gly Pro Asp Gln Trp Thr Glu Phe Ile Glu His Ile Glu
65 70 75 80
Glu Leu Ile Gly Gln Arg Ile Thr Val Val Val Arg Asn Thr Ala Ile
85 90 95
Arg Glu Leu Glu Gly Met Ala Arg Val Tyr Gln Thr Tyr Ala Thr Ala
100 105 110
Phe Ala Ala Trp Glu Lys Asp Pro Asn Asn Pro Glu Leu Arg Glu Ala
115 120 125
Leu Arg Ala Gln Phe Thr Ala Thr Glu Thr Tyr Ile Ser Gly Arg Ile
130 135 140
Ser Val Leu Thr Ile Glu Asp Tyr Gln Val Gln Leu Leu Ser Val Tyr
145 150 155 160
Ala Gln Ala Thr Asn Leu His Leu Ser Leu Leu Arg Asp Val Val Phe
165 170 175
Trp Gly Gln Arg Trp Gly Phe Ser Thr Thr Thr Leu Asn Asn Tyr Tyr
180 185 190
Ser Asp Leu Thr Arg Glu Ile Asn Glu Tyr Thr Asn Tyr Ala Val His
195 200 205
Trp Tyr Asn Val Gly Leu Glu Gln Leu Gln Gly Pro Ser Phe Gln Glu
210 215 220
Trp Val Ala Tyr Asn Arg Tyr Arg Arg Glu Leu Thr Leu Thr Val Leu
225 230 235 240
Asp Ile Val Thr Leu Phe His Asn Tyr Asp Ile Arg Leu Tyr Pro Ile
245 250 255
Pro Thr Ile Ser Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Ile Val
260 265 270
Ser Gly Ile Gly Gln Pro Asn Ser Trp Asp Phe Pro Thr Phe Ser Glu
275 280 285
Ala Glu Asn Lys Ser Ile Arg Thr Pro His Leu Met Asp Phe Leu Arg
290 295 300
Asn Leu Thr Ile Phe Thr Asp Ser Ala Arg Tyr Gly Ala Ile Tyr His
305 310 315 320
Phe Trp Gly Gly His Gln Ile Ser Ser Ser Leu Val Gly Gly Ser Asn
325 330 335
Ile Thr Phe Pro Thr Tyr Gly Ser Asn Val Ser Gln Gly Ser Pro Trp
340 345 350
Ile Leu Val Thr Asn Gly Ile Pro Ile Tyr Arg Thr Leu Ser Asn Pro
355 360 365
Tyr Tyr Arg Phe Leu Phe Gln Ser Val Gly Ser Ala Arg Leu Arg Cys
370 375 380
Val Leu Gly Val Gln Phe His Met Asp Asn Arg Ala Phe Thr Tyr Arg
385 390 395 400
Glu Lys Gly Thr Val Asp Ser Phe Asp Glu Leu Pro Pro Thr Asp Ala
405 410 415
Ser Val Ser Pro Ser Glu Gly Tyr Ser His Arg Leu Cys His Ala Thr
420 425 430
Leu Phe Gln Val Arg Thr Gly Gly Gly Gly Ala Val Ser Phe Ser Arg
435 440 445
Thr Asp Gly Val Val Phe Ser Trp Thr His Arg Ser Ala Thr Pro Thr
450 455 460
Asn Thr Ile Asp Pro Asn Val Ile Thr Gln Ile Pro Ala Val Lys Gly
465 470 475 480
Arg Ser Leu Phe Asn Gly Ala Val Ile Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Leu Val Arg Leu Asn Arg Asn Asn Gly Asn Ile Gln Asn Arg
500 505 510
Gly His Leu Pro Ile Pro Ile Gln Phe Ser Ser Arg Ser Thr Arg Tyr
515 520 525
Arg Val Arg Leu Arg Tyr Ala Ser Ala Thr Pro Ile Gln Val Asn Val
530 535 540
His Trp Glu Asn Ser Thr Ile Phe Ser Gly Ile Val Pro Ala Thr Ala
545 550 555 560
Gln Ser Leu Asp Lys Leu Gln Ser Asn Asp Phe Gly Tyr Phe Glu Ile
565 570 575
Ala Asn Thr Ile Ser Ser Ser Leu Asp Gly Ile Val Gly Ile Arg Asn
580 585 590
Phe Ser Ala Asn Ala Asp Leu Ile Ile Asp Arg Phe Glu Phe Ile Pro
595 600 605
Val
<210> 51
<211> 571
<212> PRT
<213> 苏云金芽孢杆菌
<400> 51
Met Ser Val Ser Lys Phe Leu Leu Thr Glu Leu Ile Pro Gly Leu Gly
1 5 10 15
Phe Val Phe Gly Leu Leu Asp Ala Ile Trp Gly Phe Ile Gly Pro Asp
20 25 30
Gln Trp Thr Glu Phe Ile Glu His Ile Glu Glu Leu Ile Gly Gln Arg
35 40 45
Ile Thr Val Val Val Arg Asn Thr Ala Ile Arg Glu Leu Glu Gly Met
50 55 60
Ala Arg Val Tyr Gln Thr Tyr Ala Thr Ala Phe Ala Ala Trp Glu Lys
65 70 75 80
Asp Pro Asn Asn Pro Glu Leu Arg Glu Ala Leu Arg Ala Gln Phe Thr
85 90 95
Ala Thr Glu Thr Tyr Ile Ser Gly Arg Ile Ser Val Leu Thr Ile Glu
100 105 110
Asp Tyr Gln Val Gln Leu Leu Ser Val Tyr Ala Gln Ala Thr Asn Leu
115 120 125
His Leu Ser Leu Leu Arg Asp Val Val Phe Trp Gly Gln Arg Trp Gly
130 135 140
Phe Ser Thr Thr Thr Leu Asn Asn Tyr Tyr Ser Asp Leu Thr Arg Glu
145 150 155 160
Ile Asn Glu Tyr Thr Asn Tyr Ala Val His Trp Tyr Asn Val Gly Leu
165 170 175
Glu Gln Leu Gln Gly Pro Ser Phe Gln Glu Trp Val Ala Tyr Asn Arg
180 185 190
Tyr Arg Arg Glu Leu Thr Leu Thr Val Leu Asp Ile Val Thr Leu Phe
195 200 205
His Asn Tyr Asp Ile Arg Leu Tyr Pro Ile Pro Thr Ile Ser Gln Leu
210 215 220
Thr Arg Glu Val Tyr Thr Asp Pro Ile Val Ser Gly Ile Gly Gln Pro
225 230 235 240
Asn Ser Trp Asp Phe Pro Thr Phe Ser Glu Ala Glu Asn Lys Ser Ile
245 250 255
Arg Thr Pro His Leu Met Asp Phe Leu Arg Asn Leu Thr Ile Phe Thr
260 265 270
Asp Ser Ala Arg Tyr Gly Ala Ile Tyr His Phe Trp Gly Gly His Gln
275 280 285
Ile Ser Ser Ser Leu Val Gly Gly Ser Asn Ile Thr Phe Pro Thr Tyr
290 295 300
Gly Ser Asn Val Ser Gln Gly Ser Pro Trp Ile Leu Val Thr Asn Gly
305 310 315 320
Ile Pro Ile Tyr Arg Thr Leu Ser Asn Pro Tyr Tyr Arg Phe Leu Phe
325 330 335
Gln Ser Val Gly Ser Ala Arg Leu Arg Cys Val Leu Gly Val Gln Phe
340 345 350
His Met Asp Asn Arg Ala Phe Thr Tyr Arg Glu Lys Gly Thr Val Asp
355 360 365
Ser Phe Asp Glu Leu Pro Pro Thr Asp Ala Ser Val Ser Pro Ser Glu
370 375 380
Gly Tyr Ser His Arg Leu Cys His Ala Thr Leu Phe Gln Val Arg Thr
385 390 395 400
Gly Gly Gly Gly Ala Val Ser Phe Ser Arg Thr Asp Gly Val Val Phe
405 410 415
Ser Trp Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Asn
420 425 430
Val Ile Thr Gln Ile Pro Ala Val Lys Gly Arg Ser Leu Phe Asn Gly
435 440 445
Ala Val Ile Lys Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Arg Leu
450 455 460
Asn Arg Asn Asn Gly Asn Ile Gln Asn Arg Gly His Leu Pro Ile Pro
465 470 475 480
Ile Gln Phe Ser Ser Arg Ser Thr Arg Tyr Arg Val Arg Leu Arg Tyr
485 490 495
Ala Ser Ala Thr Pro Ile Gln Val Asn Val His Trp Glu Asn Ser Thr
500 505 510
Ile Phe Ser Gly Ile Val Pro Ala Thr Ala Gln Ser Leu Asp Lys Leu
515 520 525
Gln Ser Asn Asp Phe Gly Tyr Phe Glu Ile Ala Asn Thr Ile Ser Ser
530 535 540
Ser Leu Asp Gly Ile Val Gly Ile Arg Asn Phe Ser Ala Asn Ala Asp
545 550 555 560
Leu Ile Ile Asp Arg Phe Glu Phe Ile Pro Val
565 570
<210> 52
<211> 790
<212> PRT
<213> 苏云金芽孢杆菌
<400> 52
Met Lys Lys Met Asn Ser Tyr Gln Asn Lys Asn Glu Tyr Glu Ile Leu
1 5 10 15
Asp Ala Ser Glu Asn Thr Val Asn Ala Leu Asn Arg Tyr Pro Phe Ala
20 25 30
Asn Asn Pro Tyr Ser Ser Ile Phe Ser Ser Cys Pro Arg Ser Gly Pro
35 40 45
Gly Asn Trp Ile Asn Ile Leu Gly Asn Ala Val Ser Glu Ala Val Ser
50 55 60
Ile Ser Gln Asp Ile Ile Ser Leu Leu Thr Gln Pro Ser Ile Ser Gly
65 70 75 80
Ile Ile Ser Met Ala Phe Ser Leu Leu Ser Arg Met Ile Gly Ser Asn
85 90 95
Gly Arg Ser Ile Ser Glu Leu Ser Met Cys Asp Leu Leu Ala Ile Ile
100 105 110
Asp Leu Arg Val Asn Gln Ser Val Leu Asp Asp Gly Val Ala Asp Phe
115 120 125
Asn Gly Ser Leu Val Ile Tyr Arg Asn Tyr Leu Glu Ala Leu Gln Arg
130 135 140
Trp Asn Asn Asn Pro Asn Pro Ala Asn Ala Glu Glu Val Arg Thr Arg
145 150 155 160
Phe Arg Glu Ser Asp Thr Ile Phe Asp Leu Ile Leu Thr Gln Gly Ser
165 170 175
Leu Thr Asn Gly Gly Ser Leu Ala Arg Asn Asn Ala Gln Ile Leu Leu
180 185 190
Leu Pro Ser Phe Ala Asn Ala Ala Tyr Phe His Leu Leu Leu Leu Arg
195 200 205
Asp Ala Asn Val Tyr Gly Asn Asn Trp Gly Leu Phe Gly Val Thr Pro
210 215 220
Asn Ile Asn Tyr Glu Ser Lys Leu Leu Asn Leu Ile Arg Leu Tyr Thr
225 230 235 240
Asn Tyr Cys Thr His Trp Tyr Asn Gln Gly Leu Asn Glu Leu Arg Asn
245 250 255
Arg Gly Ser Asn Ala Thr Ala Trp Leu Glu Phe His Arg Phe Arg Arg
260 265 270
Asp Met Thr Leu Met Val Leu Asp Ile Val Ser Ser Phe Ser Ser Leu
275 280 285
Asp Ile Thr Arg Tyr Pro Arg Ala Thr Asp Phe Gln Leu Ser Arg Ile
290 295 300
Ile Tyr Thr Asp Pro Ile Gly Phe Val Asn Arg Ser Asp Pro Ser Ala
305 310 315 320
Pro Arg Thr Trp Phe Ser Phe His Asn Gln Ala Asn Phe Ser Ala Leu
325 330 335
Glu Ser Gly Ile Pro Ser Pro Ser Phe Ser Gln Phe Leu Asp Ser Met
340 345 350
Arg Ile Ser Thr Gly Pro Leu Ser Leu Pro Ala Ser Pro Asn Ile His
355 360 365
Arg Ala Arg Val Trp Tyr Gly Asn Gln Asn Asn Phe Asn Gly Ser Ser
370 375 380
Ser Gln Thr Phe Gly Glu Ile Thr Asn Asp Asn Gln Thr Ile Ser Gly
385 390 395 400
Leu Asn Ile Phe Arg Ile Asp Ser Gln Ala Val Asn Leu Asn Asn Thr
405 410 415
Thr Phe Gly Val Ser Arg Ala Glu Phe Tyr His Asp Ala Ser Gln Gly
420 425 430
Ser Gln Arg Ser Ile Tyr Gln Gly Phe Val Asp Thr Gly Gly Ala Ser
435 440 445
Thr Ala Val Ala Gln Asn Ile Gln Thr Phe Phe Pro Gly Glu Asn Ser
450 455 460
Ser Ile Pro Thr Pro Gln Asp Tyr Thr His Ile Leu Ser Arg Ser Thr
465 470 475 480
Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Gly Arg Arg Ser Ser
485 490 495
Leu Val Leu His Gly Trp Thr His Lys Ser Leu Ser Arg Gln Asn Arg
500 505 510
Val Glu Pro Asn Arg Ile Thr Gln Val Pro Ala Val Lys Ala Ser Ser
515 520 525
Pro Ser Asn Cys Thr Val Ile Ala Gly Pro Gly Phe Thr Gly Gly Asp
530 535 540
Leu Val Arg Met Ser Ser Asn Cys Ser Val Ser Tyr Asn Phe Thr Pro
545 550 555 560
Ala Asp Gln Gln Val Val Ile Arg Leu Arg Tyr Ala Cys Gln Gly Thr
565 570 575
Ala Ser Leu Arg Ile Thr Phe Gly Asn Gly Ser Ser Gln Ile Ile Pro
580 585 590
Leu Val Ser Thr Thr Ser Ser Ile Asn Asn Leu Gln Tyr Glu Asn Phe
595 600 605
Ser Phe Ala Ser Gly Pro Asn Ser Val Asn Phe Leu Ser Ala Gly Thr
610 615 620
Ser Ile Thr Ile Gln Asn Ile Ser Thr Asn Ser Asn Val Val Leu Asp
625 630 635 640
Arg Ile Glu Ile Val Pro Glu Gln Pro Ile Pro Ile Ile Pro Gly Asp
645 650 655
Tyr Gln Ile Val Thr Ala Leu Asn Asn Ser Ser Val Phe Asp Leu Asn
660 665 670
Ser Gly Thr Arg Val Thr Leu Trp Ser Asn Asn Arg Gly Ala His Gln
675 680 685
Ile Trp Asn Phe Met Tyr Asp Gln Gln Arg Asn Ala Tyr Val Ile Arg
690 695 700
Asn Val Ser Asn Pro Ser Leu Val Leu Thr Trp Asp Phe Thr Ser Pro
705 710 715 720
Asn Ser Ile Val Phe Ala Ala Pro Phe Ser Pro Gly Arg Gln Glu Gln
725 730 735
Tyr Trp Ile Ala Glu Ser Phe Gln Asn Ser Tyr Val Phe Glu Asn Leu
740 745 750
Arg Asn Thr Asn Met Val Leu Asp Val Ala Gly Gly Ser Thr Ala Ile
755 760 765
Gly Thr Asn Ile Ile Ala Phe Pro Arg His Asn Gly Asn Ala Gln Arg
770 775 780
Phe Phe Ile Arg Arg Pro
785 790
<210> 53
<211> 787
<212> PRT
<213> 苏云金芽孢杆菌
<400> 53
Met Asn Ser Tyr Gln Asn Lys Asn Glu Tyr Glu Ile Leu Asp Ala Ser
1 5 10 15
Glu Asn Thr Val Asn Ala Leu Asn Arg Tyr Pro Phe Ala Asn Asn Pro
20 25 30
Tyr Ser Ser Ile Phe Ser Ser Cys Pro Arg Ser Gly Pro Gly Asn Trp
35 40 45
Ile Asn Ile Leu Gly Asn Ala Val Ser Glu Ala Val Ser Ile Ser Gln
50 55 60
Asp Ile Ile Ser Leu Leu Thr Gln Pro Ser Ile Ser Gly Ile Ile Ser
65 70 75 80
Met Ala Phe Ser Leu Leu Ser Arg Met Ile Gly Ser Asn Gly Arg Ser
85 90 95
Ile Ser Glu Leu Ser Met Cys Asp Leu Leu Ala Ile Ile Asp Leu Arg
100 105 110
Val Asn Gln Ser Val Leu Asp Asp Gly Val Ala Asp Phe Asn Gly Ser
115 120 125
Leu Val Ile Tyr Arg Asn Tyr Leu Glu Ala Leu Gln Arg Trp Asn Asn
130 135 140
Asn Pro Asn Pro Ala Asn Ala Glu Glu Val Arg Thr Arg Phe Arg Glu
145 150 155 160
Ser Asp Thr Ile Phe Asp Leu Ile Leu Thr Gln Gly Ser Leu Thr Asn
165 170 175
Gly Gly Ser Leu Ala Arg Asn Asn Ala Gln Ile Leu Leu Leu Pro Ser
180 185 190
Phe Ala Asn Ala Ala Tyr Phe His Leu Leu Leu Leu Arg Asp Ala Asn
195 200 205
Val Tyr Gly Asn Asn Trp Gly Leu Phe Gly Val Thr Pro Asn Ile Asn
210 215 220
Tyr Glu Ser Lys Leu Leu Asn Leu Ile Arg Leu Tyr Thr Asn Tyr Cys
225 230 235 240
Thr His Trp Tyr Asn Gln Gly Leu Asn Glu Leu Arg Asn Arg Gly Ser
245 250 255
Asn Ala Thr Ala Trp Leu Glu Phe His Arg Phe Arg Arg Asp Met Thr
260 265 270
Leu Met Val Leu Asp Ile Val Ser Ser Phe Ser Ser Leu Asp Ile Thr
275 280 285
Arg Tyr Pro Arg Ala Thr Asp Phe Gln Leu Ser Arg Ile Ile Tyr Thr
290 295 300
Asp Pro Ile Gly Phe Val Asn Arg Ser Asp Pro Ser Ala Pro Arg Thr
305 310 315 320
Trp Phe Ser Phe His Asn Gln Ala Asn Phe Ser Ala Leu Glu Ser Gly
325 330 335
Ile Pro Ser Pro Ser Phe Ser Gln Phe Leu Asp Ser Met Arg Ile Ser
340 345 350
Thr Gly Pro Leu Ser Leu Pro Ala Ser Pro Asn Ile His Arg Ala Arg
355 360 365
Val Trp Tyr Gly Asn Gln Asn Asn Phe Asn Gly Ser Ser Ser Gln Thr
370 375 380
Phe Gly Glu Ile Thr Asn Asp Asn Gln Thr Ile Ser Gly Leu Asn Ile
385 390 395 400
Phe Arg Ile Asp Ser Gln Ala Val Asn Leu Asn Asn Thr Thr Phe Gly
405 410 415
Val Ser Arg Ala Glu Phe Tyr His Asp Ala Ser Gln Gly Ser Gln Arg
420 425 430
Ser Ile Tyr Gln Gly Phe Val Asp Thr Gly Gly Ala Ser Thr Ala Val
435 440 445
Ala Gln Asn Ile Gln Thr Phe Phe Pro Gly Glu Asn Ser Ser Ile Pro
450 455 460
Thr Pro Gln Asp Tyr Thr His Ile Leu Ser Arg Ser Thr Asn Leu Thr
465 470 475 480
Gly Gly Leu Arg Gln Val Ala Ser Gly Arg Arg Ser Ser Leu Val Leu
485 490 495
His Gly Trp Thr His Lys Ser Leu Ser Arg Gln Asn Arg Val Glu Pro
500 505 510
Asn Arg Ile Thr Gln Val Pro Ala Val Lys Ala Ser Ser Pro Ser Asn
515 520 525
Cys Thr Val Ile Ala Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Arg
530 535 540
Met Ser Ser Asn Cys Ser Val Ser Tyr Asn Phe Thr Pro Ala Asp Gln
545 550 555 560
Gln Val Val Ile Arg Leu Arg Tyr Ala Cys Gln Gly Thr Ala Ser Leu
565 570 575
Arg Ile Thr Phe Gly Asn Gly Ser Ser Gln Ile Ile Pro Leu Val Ser
580 585 590
Thr Thr Ser Ser Ile Asn Asn Leu Gln Tyr Glu Asn Phe Ser Phe Ala
595 600 605
Ser Gly Pro Asn Ser Val Asn Phe Leu Ser Ala Gly Thr Ser Ile Thr
610 615 620
Ile Gln Asn Ile Ser Thr Asn Ser Asn Val Val Leu Asp Arg Ile Glu
625 630 635 640
Ile Val Pro Glu Gln Pro Ile Pro Ile Ile Pro Gly Asp Tyr Gln Ile
645 650 655
Val Thr Ala Leu Asn Asn Ser Ser Val Phe Asp Leu Asn Ser Gly Thr
660 665 670
Arg Val Thr Leu Trp Ser Asn Asn Arg Gly Ala His Gln Ile Trp Asn
675 680 685
Phe Met Tyr Asp Gln Gln Arg Asn Ala Tyr Val Ile Arg Asn Val Ser
690 695 700
Asn Pro Ser Leu Val Leu Thr Trp Asp Phe Thr Ser Pro Asn Ser Ile
705 710 715 720
Val Phe Ala Ala Pro Phe Ser Pro Gly Arg Gln Glu Gln Tyr Trp Ile
725 730 735
Ala Glu Ser Phe Gln Asn Ser Tyr Val Phe Glu Asn Leu Arg Asn Thr
740 745 750
Asn Met Val Leu Asp Val Ala Gly Gly Ser Thr Ala Ile Gly Thr Asn
755 760 765
Ile Ile Ala Phe Pro Arg His Asn Gly Asn Ala Gln Arg Phe Phe Ile
770 775 780
Arg Arg Pro
785
<210> 54
<211> 707
<212> PRT
<213> 苏云金芽孢杆菌
<400> 54
Met Ala Phe Ser Leu Leu Ser Arg Met Ile Gly Ser Asn Gly Arg Ser
1 5 10 15
Ile Ser Glu Leu Ser Met Cys Asp Leu Leu Ala Ile Ile Asp Leu Arg
20 25 30
Val Asn Gln Ser Val Leu Asp Asp Gly Val Ala Asp Phe Asn Gly Ser
35 40 45
Leu Val Ile Tyr Arg Asn Tyr Leu Glu Ala Leu Gln Arg Trp Asn Asn
50 55 60
Asn Pro Asn Pro Ala Asn Ala Glu Glu Val Arg Thr Arg Phe Arg Glu
65 70 75 80
Ser Asp Thr Ile Phe Asp Leu Ile Leu Thr Gln Gly Ser Leu Thr Asn
85 90 95
Gly Gly Ser Leu Ala Arg Asn Asn Ala Gln Ile Leu Leu Leu Pro Ser
100 105 110
Phe Ala Asn Ala Ala Tyr Phe His Leu Leu Leu Leu Arg Asp Ala Asn
115 120 125
Val Tyr Gly Asn Asn Trp Gly Leu Phe Gly Val Thr Pro Asn Ile Asn
130 135 140
Tyr Glu Ser Lys Leu Leu Asn Leu Ile Arg Leu Tyr Thr Asn Tyr Cys
145 150 155 160
Thr His Trp Tyr Asn Gln Gly Leu Asn Glu Leu Arg Asn Arg Gly Ser
165 170 175
Asn Ala Thr Ala Trp Leu Glu Phe His Arg Phe Arg Arg Asp Met Thr
180 185 190
Leu Met Val Leu Asp Ile Val Ser Ser Phe Ser Ser Leu Asp Ile Thr
195 200 205
Arg Tyr Pro Arg Ala Thr Asp Phe Gln Leu Ser Arg Ile Ile Tyr Thr
210 215 220
Asp Pro Ile Gly Phe Val Asn Arg Ser Asp Pro Ser Ala Pro Arg Thr
225 230 235 240
Trp Phe Ser Phe His Asn Gln Ala Asn Phe Ser Ala Leu Glu Ser Gly
245 250 255
Ile Pro Ser Pro Ser Phe Ser Gln Phe Leu Asp Ser Met Arg Ile Ser
260 265 270
Thr Gly Pro Leu Ser Leu Pro Ala Ser Pro Asn Ile His Arg Ala Arg
275 280 285
Val Trp Tyr Gly Asn Gln Asn Asn Phe Asn Gly Ser Ser Ser Gln Thr
290 295 300
Phe Gly Glu Ile Thr Asn Asp Asn Gln Thr Ile Ser Gly Leu Asn Ile
305 310 315 320
Phe Arg Ile Asp Ser Gln Ala Val Asn Leu Asn Asn Thr Thr Phe Gly
325 330 335
Val Ser Arg Ala Glu Phe Tyr His Asp Ala Ser Gln Gly Ser Gln Arg
340 345 350
Ser Ile Tyr Gln Gly Phe Val Asp Thr Gly Gly Ala Ser Thr Ala Val
355 360 365
Ala Gln Asn Ile Gln Thr Phe Phe Pro Gly Glu Asn Ser Ser Ile Pro
370 375 380
Thr Pro Gln Asp Tyr Thr His Ile Leu Ser Arg Ser Thr Asn Leu Thr
385 390 395 400
Gly Gly Leu Arg Gln Val Ala Ser Gly Arg Arg Ser Ser Leu Val Leu
405 410 415
His Gly Trp Thr His Lys Ser Leu Ser Arg Gln Asn Arg Val Glu Pro
420 425 430
Asn Arg Ile Thr Gln Val Pro Ala Val Lys Ala Ser Ser Pro Ser Asn
435 440 445
Cys Thr Val Ile Ala Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Arg
450 455 460
Met Ser Ser Asn Cys Ser Val Ser Tyr Asn Phe Thr Pro Ala Asp Gln
465 470 475 480
Gln Val Val Ile Arg Leu Arg Tyr Ala Cys Gln Gly Thr Ala Ser Leu
485 490 495
Arg Ile Thr Phe Gly Asn Gly Ser Ser Gln Ile Ile Pro Leu Val Ser
500 505 510
Thr Thr Ser Ser Ile Asn Asn Leu Gln Tyr Glu Asn Phe Ser Phe Ala
515 520 525
Ser Gly Pro Asn Ser Val Asn Phe Leu Ser Ala Gly Thr Ser Ile Thr
530 535 540
Ile Gln Asn Ile Ser Thr Asn Ser Asn Val Val Leu Asp Arg Ile Glu
545 550 555 560
Ile Val Pro Glu Gln Pro Ile Pro Ile Ile Pro Gly Asp Tyr Gln Ile
565 570 575
Val Thr Ala Leu Asn Asn Ser Ser Val Phe Asp Leu Asn Ser Gly Thr
580 585 590
Arg Val Thr Leu Trp Ser Asn Asn Arg Gly Ala His Gln Ile Trp Asn
595 600 605
Phe Met Tyr Asp Gln Gln Arg Asn Ala Tyr Val Ile Arg Asn Val Ser
610 615 620
Asn Pro Ser Leu Val Leu Thr Trp Asp Phe Thr Ser Pro Asn Ser Ile
625 630 635 640
Val Phe Ala Ala Pro Phe Ser Pro Gly Arg Gln Glu Gln Tyr Trp Ile
645 650 655
Ala Glu Ser Phe Gln Asn Ser Tyr Val Phe Glu Asn Leu Arg Asn Thr
660 665 670
Asn Met Val Leu Asp Val Ala Gly Gly Ser Thr Ala Ile Gly Thr Asn
675 680 685
Ile Ile Ala Phe Pro Arg His Asn Gly Asn Ala Gln Arg Phe Phe Ile
690 695 700
Arg Arg Pro
705
<210> 55
<211> 699
<212> PRT
<213> 苏云金芽孢杆菌
<400> 55
Met Ile Gly Ser Asn Gly Arg Ser Ile Ser Glu Leu Ser Met Cys Asp
1 5 10 15
Leu Leu Ala Ile Ile Asp Leu Arg Val Asn Gln Ser Val Leu Asp Asp
20 25 30
Gly Val Ala Asp Phe Asn Gly Ser Leu Val Ile Tyr Arg Asn Tyr Leu
35 40 45
Glu Ala Leu Gln Arg Trp Asn Asn Asn Pro Asn Pro Ala Asn Ala Glu
50 55 60
Glu Val Arg Thr Arg Phe Arg Glu Ser Asp Thr Ile Phe Asp Leu Ile
65 70 75 80
Leu Thr Gln Gly Ser Leu Thr Asn Gly Gly Ser Leu Ala Arg Asn Asn
85 90 95
Ala Gln Ile Leu Leu Leu Pro Ser Phe Ala Asn Ala Ala Tyr Phe His
100 105 110
Leu Leu Leu Leu Arg Asp Ala Asn Val Tyr Gly Asn Asn Trp Gly Leu
115 120 125
Phe Gly Val Thr Pro Asn Ile Asn Tyr Glu Ser Lys Leu Leu Asn Leu
130 135 140
Ile Arg Leu Tyr Thr Asn Tyr Cys Thr His Trp Tyr Asn Gln Gly Leu
145 150 155 160
Asn Glu Leu Arg Asn Arg Gly Ser Asn Ala Thr Ala Trp Leu Glu Phe
165 170 175
His Arg Phe Arg Arg Asp Met Thr Leu Met Val Leu Asp Ile Val Ser
180 185 190
Ser Phe Ser Ser Leu Asp Ile Thr Arg Tyr Pro Arg Ala Thr Asp Phe
195 200 205
Gln Leu Ser Arg Ile Ile Tyr Thr Asp Pro Ile Gly Phe Val Asn Arg
210 215 220
Ser Asp Pro Ser Ala Pro Arg Thr Trp Phe Ser Phe His Asn Gln Ala
225 230 235 240
Asn Phe Ser Ala Leu Glu Ser Gly Ile Pro Ser Pro Ser Phe Ser Gln
245 250 255
Phe Leu Asp Ser Met Arg Ile Ser Thr Gly Pro Leu Ser Leu Pro Ala
260 265 270
Ser Pro Asn Ile His Arg Ala Arg Val Trp Tyr Gly Asn Gln Asn Asn
275 280 285
Phe Asn Gly Ser Ser Ser Gln Thr Phe Gly Glu Ile Thr Asn Asp Asn
290 295 300
Gln Thr Ile Ser Gly Leu Asn Ile Phe Arg Ile Asp Ser Gln Ala Val
305 310 315 320
Asn Leu Asn Asn Thr Thr Phe Gly Val Ser Arg Ala Glu Phe Tyr His
325 330 335
Asp Ala Ser Gln Gly Ser Gln Arg Ser Ile Tyr Gln Gly Phe Val Asp
340 345 350
Thr Gly Gly Ala Ser Thr Ala Val Ala Gln Asn Ile Gln Thr Phe Phe
355 360 365
Pro Gly Glu Asn Ser Ser Ile Pro Thr Pro Gln Asp Tyr Thr His Ile
370 375 380
Leu Ser Arg Ser Thr Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser
385 390 395 400
Gly Arg Arg Ser Ser Leu Val Leu His Gly Trp Thr His Lys Ser Leu
405 410 415
Ser Arg Gln Asn Arg Val Glu Pro Asn Arg Ile Thr Gln Val Pro Ala
420 425 430
Val Lys Ala Ser Ser Pro Ser Asn Cys Thr Val Ile Ala Gly Pro Gly
435 440 445
Phe Thr Gly Gly Asp Leu Val Arg Met Ser Ser Asn Cys Ser Val Ser
450 455 460
Tyr Asn Phe Thr Pro Ala Asp Gln Gln Val Val Ile Arg Leu Arg Tyr
465 470 475 480
Ala Cys Gln Gly Thr Ala Ser Leu Arg Ile Thr Phe Gly Asn Gly Ser
485 490 495
Ser Gln Ile Ile Pro Leu Val Ser Thr Thr Ser Ser Ile Asn Asn Leu
500 505 510
Gln Tyr Glu Asn Phe Ser Phe Ala Ser Gly Pro Asn Ser Val Asn Phe
515 520 525
Leu Ser Ala Gly Thr Ser Ile Thr Ile Gln Asn Ile Ser Thr Asn Ser
530 535 540
Asn Val Val Leu Asp Arg Ile Glu Ile Val Pro Glu Gln Pro Ile Pro
545 550 555 560
Ile Ile Pro Gly Asp Tyr Gln Ile Val Thr Ala Leu Asn Asn Ser Ser
565 570 575
Val Phe Asp Leu Asn Ser Gly Thr Arg Val Thr Leu Trp Ser Asn Asn
580 585 590
Arg Gly Ala His Gln Ile Trp Asn Phe Met Tyr Asp Gln Gln Arg Asn
595 600 605
Ala Tyr Val Ile Arg Asn Val Ser Asn Pro Ser Leu Val Leu Thr Trp
610 615 620
Asp Phe Thr Ser Pro Asn Ser Ile Val Phe Ala Ala Pro Phe Ser Pro
625 630 635 640
Gly Arg Gln Glu Gln Tyr Trp Ile Ala Glu Ser Phe Gln Asn Ser Tyr
645 650 655
Val Phe Glu Asn Leu Arg Asn Thr Asn Met Val Leu Asp Val Ala Gly
660 665 670
Gly Ser Thr Ala Ile Gly Thr Asn Ile Ile Ala Phe Pro Arg His Asn
675 680 685
Gly Asn Ala Gln Arg Phe Phe Ile Arg Arg Pro
690 695
<210> 56
<211> 686
<212> PRT
<213> 苏云金芽孢杆菌
<400> 56
Met Cys Asp Leu Leu Ala Ile Ile Asp Leu Arg Val Asn Gln Ser Val
1 5 10 15
Leu Asp Asp Gly Val Ala Asp Phe Asn Gly Ser Leu Val Ile Tyr Arg
20 25 30
Asn Tyr Leu Glu Ala Leu Gln Arg Trp Asn Asn Asn Pro Asn Pro Ala
35 40 45
Asn Ala Glu Glu Val Arg Thr Arg Phe Arg Glu Ser Asp Thr Ile Phe
50 55 60
Asp Leu Ile Leu Thr Gln Gly Ser Leu Thr Asn Gly Gly Ser Leu Ala
65 70 75 80
Arg Asn Asn Ala Gln Ile Leu Leu Leu Pro Ser Phe Ala Asn Ala Ala
85 90 95
Tyr Phe His Leu Leu Leu Leu Arg Asp Ala Asn Val Tyr Gly Asn Asn
100 105 110
Trp Gly Leu Phe Gly Val Thr Pro Asn Ile Asn Tyr Glu Ser Lys Leu
115 120 125
Leu Asn Leu Ile Arg Leu Tyr Thr Asn Tyr Cys Thr His Trp Tyr Asn
130 135 140
Gln Gly Leu Asn Glu Leu Arg Asn Arg Gly Ser Asn Ala Thr Ala Trp
145 150 155 160
Leu Glu Phe His Arg Phe Arg Arg Asp Met Thr Leu Met Val Leu Asp
165 170 175
Ile Val Ser Ser Phe Ser Ser Leu Asp Ile Thr Arg Tyr Pro Arg Ala
180 185 190
Thr Asp Phe Gln Leu Ser Arg Ile Ile Tyr Thr Asp Pro Ile Gly Phe
195 200 205
Val Asn Arg Ser Asp Pro Ser Ala Pro Arg Thr Trp Phe Ser Phe His
210 215 220
Asn Gln Ala Asn Phe Ser Ala Leu Glu Ser Gly Ile Pro Ser Pro Ser
225 230 235 240
Phe Ser Gln Phe Leu Asp Ser Met Arg Ile Ser Thr Gly Pro Leu Ser
245 250 255
Leu Pro Ala Ser Pro Asn Ile His Arg Ala Arg Val Trp Tyr Gly Asn
260 265 270
Gln Asn Asn Phe Asn Gly Ser Ser Ser Gln Thr Phe Gly Glu Ile Thr
275 280 285
Asn Asp Asn Gln Thr Ile Ser Gly Leu Asn Ile Phe Arg Ile Asp Ser
290 295 300
Gln Ala Val Asn Leu Asn Asn Thr Thr Phe Gly Val Ser Arg Ala Glu
305 310 315 320
Phe Tyr His Asp Ala Ser Gln Gly Ser Gln Arg Ser Ile Tyr Gln Gly
325 330 335
Phe Val Asp Thr Gly Gly Ala Ser Thr Ala Val Ala Gln Asn Ile Gln
340 345 350
Thr Phe Phe Pro Gly Glu Asn Ser Ser Ile Pro Thr Pro Gln Asp Tyr
355 360 365
Thr His Ile Leu Ser Arg Ser Thr Asn Leu Thr Gly Gly Leu Arg Gln
370 375 380
Val Ala Ser Gly Arg Arg Ser Ser Leu Val Leu His Gly Trp Thr His
385 390 395 400
Lys Ser Leu Ser Arg Gln Asn Arg Val Glu Pro Asn Arg Ile Thr Gln
405 410 415
Val Pro Ala Val Lys Ala Ser Ser Pro Ser Asn Cys Thr Val Ile Ala
420 425 430
Gly Pro Gly Phe Thr Gly Gly Asp Leu Val Arg Met Ser Ser Asn Cys
435 440 445
Ser Val Ser Tyr Asn Phe Thr Pro Ala Asp Gln Gln Val Val Ile Arg
450 455 460
Leu Arg Tyr Ala Cys Gln Gly Thr Ala Ser Leu Arg Ile Thr Phe Gly
465 470 475 480
Asn Gly Ser Ser Gln Ile Ile Pro Leu Val Ser Thr Thr Ser Ser Ile
485 490 495
Asn Asn Leu Gln Tyr Glu Asn Phe Ser Phe Ala Ser Gly Pro Asn Ser
500 505 510
Val Asn Phe Leu Ser Ala Gly Thr Ser Ile Thr Ile Gln Asn Ile Ser
515 520 525
Thr Asn Ser Asn Val Val Leu Asp Arg Ile Glu Ile Val Pro Glu Gln
530 535 540
Pro Ile Pro Ile Ile Pro Gly Asp Tyr Gln Ile Val Thr Ala Leu Asn
545 550 555 560
Asn Ser Ser Val Phe Asp Leu Asn Ser Gly Thr Arg Val Thr Leu Trp
565 570 575
Ser Asn Asn Arg Gly Ala His Gln Ile Trp Asn Phe Met Tyr Asp Gln
580 585 590
Gln Arg Asn Ala Tyr Val Ile Arg Asn Val Ser Asn Pro Ser Leu Val
595 600 605
Leu Thr Trp Asp Phe Thr Ser Pro Asn Ser Ile Val Phe Ala Ala Pro
610 615 620
Phe Ser Pro Gly Arg Gln Glu Gln Tyr Trp Ile Ala Glu Ser Phe Gln
625 630 635 640
Asn Ser Tyr Val Phe Glu Asn Leu Arg Asn Thr Asn Met Val Leu Asp
645 650 655
Val Ala Gly Gly Ser Thr Ala Ile Gly Thr Asn Ile Ile Ala Phe Pro
660 665 670
Arg His Asn Gly Asn Ala Gln Arg Phe Phe Ile Arg Arg Pro
675 680 685
<210> 57
<211> 1172
<212> PRT
<213> 苏云金芽孢杆菌
<400> 57
Met Glu Val Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Asn
1 5 10 15
Asn Pro Glu Ile Glu Ile Leu Gly Gly Glu Arg Ile Ser Val Gly Asn
20 25 30
Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser Glu
35 40 45
Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Ile Asp Leu Ile Trp
50 55 60
Gly Phe Leu Gly Pro Ser Gln Trp Asp Ala Phe Leu Leu Gln Ile Glu
65 70 75 80
Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95
Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Arg Ile Tyr Ala Glu Ala
100 105 110
Phe Arg Ala Trp Glu Ala Asp Pro Thr Asn Leu Ala Leu Arg Glu Glu
115 120 125
Met Arg Thr Gln Phe Asn Asp Met Asn Ser Ala Leu Val Thr Ala Ile
130 135 140
Pro Leu Phe Ser Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val Tyr
145 150 155 160
Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser Val
165 170 175
Phe Gly Gln Arg Trp Gly Phe Asp Val Ala Thr Ile Asn Ser Arg Tyr
180 185 190
Asn Asp Leu Thr Arg Leu Ile Gly Glu Tyr Thr Asp Tyr Ala Val Arg
195 200 205
Trp Tyr Asn Thr Gly Leu Asp Arg Leu Arg Gly Ser Asn Phe Gln Asp
210 215 220
Trp Ile Arg Tyr Asn Arg Phe Arg Arg Glu Leu Thr Leu Thr Val Leu
225 230 235 240
Asp Ile Val Ser Val Phe Gln Asn Tyr Asp Ser Arg Leu Tyr Pro Ile
245 250 255
Gln Thr Ser Ser Gln Leu Thr Arg Glu Ile Tyr Ser Asp Leu Leu Leu
260 265 270
Ala Asn Pro Ser Gly Val Gly Ser Phe Ser Asn Val Asp Phe Asp Ser
275 280 285
Ile Leu Ile Arg Gln Pro His Leu Ile Asp Phe Met Arg Val Leu Thr
290 295 300
Ile Tyr Thr Asp Arg His Asn Ala Ser Arg His Asn Ile Tyr Trp Ala
305 310 315 320
Gly His Gln Val Thr Ala Val Asp Thr Ala Asn Arg Thr Ile Val Tyr
325 330 335
Pro Val Asn Gly Ser Ala Ala Asn Leu Glu Pro Pro Arg Thr Leu Arg
340 345 350
Phe Glu Ser Pro Val Val Glu Ile Arg Ser Asn Pro Val Trp Asp Arg
355 360 365
Gly Ser Thr Gly Ile Ala Gly Ser Tyr Glu Phe Phe Gly Val Thr Ser
370 375 380
Ala Leu Phe Ile Thr Ile Leu Gly Phe Gly Tyr Thr Tyr Arg Ser Gly
385 390 395 400
Ser Asn Thr Glu Val Thr Ala Leu Pro Asp His Gln Val Ser His Ile
405 410 415
Gly Tyr Phe Arg Arg Phe Thr Thr Thr Gly Ala Thr Ala Arg Gln Thr
420 425 430
Leu Thr Ser Ala Pro Ile Val Ser Trp Thr His Ser Ser Ala Glu Pro
435 440 445
Pro Asn Arg Ile Tyr Gln Asn Arg Ile Thr Gln Ile Pro Ala Val Lys
450 455 460
Gly Asn Phe Leu Phe Asn Gly Ala Val Ile Ser Gly Pro Gly Phe Thr
465 470 475 480
Gly Gly Asp Leu Val Arg Leu Asn Arg Asn Asn Asp Asn Ile Gln Asn
485 490 495
Arg Gly Tyr Ile Glu Val Pro Ile Gln Phe Ala Ser Thr Ser Thr Arg
500 505 510
Tyr Arg Val Arg Val Arg Tyr Ala Ser Thr Asn Ala Ile Glu Val Asn
515 520 525
Ile Asn Trp Gly Asn Gly Ser Ile Phe Thr Gly Thr Ala Pro Ala Thr
530 535 540
Ala Thr Ser Leu Asp Asn Leu Gln Ser Asn Asp Phe Gly Tyr Phe Glu
545 550 555 560
Ser Thr Thr Ala Phe Ala Pro Ser Leu Gly Asn Ile Val Gly Val Arg
565 570 575
Asn Phe Ser Ala Asn Ala Asp Val Ile Ile Asp Arg Phe Glu Phe Ile
580 585 590
Pro Val Thr Ala Thr Leu Glu Ala Glu Tyr Asp Leu Glu Arg Ala Glu
595 600 605
Lys Ala Val Asn Ala Leu Phe Thr Ser Thr Thr Gln Leu Gly Leu Lys
610 615 620
Thr Asp Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu
625 630 635 640
Cys Leu Ser Asp Glu Phe Cys Leu Asn Glu Lys Arg Glu Leu Ser Glu
645 650 655
Lys Val Lys His Ala Lys Arg Leu Ser Asp Lys Arg Asn Leu Leu Gln
660 665 670
Asp Pro Asn Phe Thr Ser Ile Asn Gly Gln Leu Asp Arg Gly Trp Arg
675 680 685
Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly Asn Asp Val Phe Lys Glu
690 695 700
Asn Tyr Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr
705 710 715 720
Leu Tyr Gln Lys Ile Asp Glu Ser Gln Leu Lys Ser Tyr Thr Arg Tyr
725 730 735
Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu
740 745 750
Ile Arg Tyr Asn Ala Lys His Glu Thr Leu Ser Val Pro Gly Thr Glu
755 760 765
Ser Pro Trp Pro Ser Ser Gly Val Tyr Pro Ile Gly Lys Cys Gly Glu
770 775 780
Pro Asn Arg Cys Ala Pro Arg Ile Glu Trp Asn Pro Asp Leu Gly Cys
785 790 795 800
Ser Cys Arg Tyr Gly Glu Lys Cys Val His His Ser His His Phe Ser
805 810 815
Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val
820 825 830
Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Lys Leu Gly
835 840 845
Asn Leu Glu Phe Ile Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ser
850 855 860
Arg Val Lys Arg Ala Glu Lys Lys Trp Lys Asp Lys Cys Glu Lys Leu
865 870 875 880
Gln Leu Glu Thr Gln Arg Val Tyr Thr Glu Ala Lys Glu Ser Val Asp
885 890 895
Ala Leu Phe Ile Asp Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn
900 905 910
Ile Gly Met Ile His Ala Ala Asp Lys Gln Val His Arg Ile Arg Glu
915 920 925
Ala Tyr Leu Pro Glu Leu His Ala Ile Pro Gly Val Asn Ala Glu Ile
930 935 940
Phe Glu Glu Leu Glu Asn Phe Arg Ile Tyr Thr Ala Phe Ser Leu Tyr
945 950 955 960
Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser
965 970 975
Cys Trp Asn Val Lys Gly His Val Asp Val Gln Gln Asn His His Arg
980 985 990
Ser Val Leu Val Leu Ser Glu Trp Glu Ala Glu Val Ser Gln Lys Val
995 1000 1005
Arg Val Cys Pro Asp Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr
1010 1015 1020
Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Phe Glu
1025 1030 1035
Asp Asn Thr Asp Val Leu Lys Phe Arg Asn Cys Val Glu Glu Glu
1040 1045 1050
Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Thr Asn
1055 1060 1065
Gln Ser Ala Glu Gly Cys Thr Asp Ala Cys Asn Ser Tyr Asn Arg
1070 1075 1080
Gly Tyr Glu Asp Gly Tyr Gly Asn Asn Pro Ser Ala Pro Val Asn
1085 1090 1095
Tyr Thr Pro Thr Tyr Glu Glu Arg Met Tyr Thr Asp Thr Asp Thr
1100 1105 1110
Gln Gly Tyr Asn His Cys Val Ser Asp Arg Gly Tyr Arg Asn His
1115 1120 1125
Thr Pro Leu Pro Ala Gly Tyr Val Thr Leu Glu Leu Glu Phe Phe
1130 1135 1140
Pro Glu Thr Glu Gln Val Trp Ile Glu Ile Gly Glu Thr Glu Gly
1145 1150 1155
Thr Phe Ile Val Asp Ser Val Glu Leu Phe Leu Met Glu Glu
1160 1165 1170
<210> 58
<211> 594
<212> PRT
<213> 苏云金芽孢杆菌
<400> 58
Met Glu Val Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Asn
1 5 10 15
Asn Pro Glu Ile Glu Ile Leu Gly Gly Glu Arg Ile Ser Val Gly Asn
20 25 30
Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser Glu
35 40 45
Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Ile Asp Leu Ile Trp
50 55 60
Gly Phe Leu Gly Pro Ser Gln Trp Asp Ala Phe Leu Leu Gln Ile Glu
65 70 75 80
Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95
Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Arg Ile Tyr Ala Glu Ala
100 105 110
Phe Arg Ala Trp Glu Ala Asp Pro Thr Asn Leu Ala Leu Arg Glu Glu
115 120 125
Met Arg Thr Gln Phe Asn Asp Met Asn Ser Ala Leu Val Thr Ala Ile
130 135 140
Pro Leu Phe Ser Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val Tyr
145 150 155 160
Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser Val
165 170 175
Phe Gly Gln Arg Trp Gly Phe Asp Val Ala Thr Ile Asn Ser Arg Tyr
180 185 190
Asn Asp Leu Thr Arg Leu Ile Gly Glu Tyr Thr Asp Tyr Ala Val Arg
195 200 205
Trp Tyr Asn Thr Gly Leu Asp Arg Leu Arg Gly Ser Asn Phe Gln Asp
210 215 220
Trp Ile Arg Tyr Asn Arg Phe Arg Arg Glu Leu Thr Leu Thr Val Leu
225 230 235 240
Asp Ile Val Ser Val Phe Gln Asn Tyr Asp Ser Arg Leu Tyr Pro Ile
245 250 255
Gln Thr Ser Ser Gln Leu Thr Arg Glu Ile Tyr Ser Asp Leu Leu Leu
260 265 270
Ala Asn Pro Ser Gly Val Gly Ser Phe Ser Asn Val Asp Phe Asp Ser
275 280 285
Ile Leu Ile Arg Gln Pro His Leu Ile Asp Phe Met Arg Val Leu Thr
290 295 300
Ile Tyr Thr Asp Arg His Asn Ala Ser Arg His Asn Ile Tyr Trp Ala
305 310 315 320
Gly His Gln Val Thr Ala Val Asp Thr Ala Asn Arg Thr Ile Val Tyr
325 330 335
Pro Val Asn Gly Ser Ala Ala Asn Leu Glu Pro Pro Arg Thr Leu Arg
340 345 350
Phe Glu Ser Pro Val Val Glu Ile Arg Ser Asn Pro Val Trp Asp Arg
355 360 365
Gly Ser Thr Gly Ile Ala Gly Ser Tyr Glu Phe Phe Gly Val Thr Ser
370 375 380
Ala Leu Phe Ile Thr Ile Leu Gly Phe Gly Tyr Thr Tyr Arg Ser Gly
385 390 395 400
Ser Asn Thr Glu Val Thr Ala Leu Pro Asp His Gln Val Ser His Ile
405 410 415
Gly Tyr Phe Arg Arg Phe Thr Thr Thr Gly Ala Thr Ala Arg Gln Thr
420 425 430
Leu Thr Ser Ala Pro Ile Val Ser Trp Thr His Ser Ser Ala Glu Pro
435 440 445
Pro Asn Arg Ile Tyr Gln Asn Arg Ile Thr Gln Ile Pro Ala Val Lys
450 455 460
Gly Asn Phe Leu Phe Asn Gly Ala Val Ile Ser Gly Pro Gly Phe Thr
465 470 475 480
Gly Gly Asp Leu Val Arg Leu Asn Arg Asn Asn Asp Asn Ile Gln Asn
485 490 495
Arg Gly Tyr Ile Glu Val Pro Ile Gln Phe Ala Ser Thr Ser Thr Arg
500 505 510
Tyr Arg Val Arg Val Arg Tyr Ala Ser Thr Asn Ala Ile Glu Val Asn
515 520 525
Ile Asn Trp Gly Asn Gly Ser Ile Phe Thr Gly Thr Ala Pro Ala Thr
530 535 540
Ala Thr Ser Leu Asp Asn Leu Gln Ser Asn Asp Phe Gly Tyr Phe Glu
545 550 555 560
Ser Thr Thr Ala Phe Ala Pro Ser Leu Gly Asn Ile Val Gly Val Arg
565 570 575
Asn Phe Ser Ala Asn Ala Asp Val Ile Ile Asp Arg Phe Glu Phe Ile
580 585 590
Pro Val
<210> 59
<211> 333
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 59
Met Lys Val Tyr Lys Lys Ile Thr Lys Met Ala Pro Ile Met Ala Leu
1 5 10 15
Ser Thr Ala Val Leu Leu Ser Pro Gly Ser Thr Phe Ala Ala Glu Lys
20 25 30
Ala Val Val Thr Lys Ser Asn Val Ser Ser Leu Thr Thr Asn Thr Val
35 40 45
Met Gln Ser Gly Ser Ile Ile Gln Gly Tyr Leu Ile Lys Asn Gly Val
50 55 60
Lys Thr Pro Val Tyr Asn Ser Glu Val Gln Thr Arg Ser Thr Ala Val
65 70 75 80
Asn Glu Ala Pro Tyr Pro Glu Leu Ser Ser Asn Pro Asn Asp Pro Val
85 90 95
Pro Ser Lys Gly Ser Ile Thr Ser Glu Ser Gly Asn Val Gly Ser Val
100 105 110
Leu Tyr Phe Ser Lys Phe Asn Ser Gln Lys Leu Gln Asn Thr Ala Glu
115 120 125
Pro Val Tyr Trp Lys Asn Val Tyr Leu Glu Lys Thr Pro Asp Gly Asn
130 135 140
Ile Ile Phe Gly Thr Tyr Asp Pro Thr Thr Leu Lys Arg Thr Pro Asn
145 150 155 160
Leu Val Asn Ile Met Met Thr Pro Ser Lys Val Gln Tyr Tyr Gln Ser
165 170 175
Phe Phe Thr Asp Thr Lys Ile Lys Arg Glu Thr Ala Tyr Glu Lys Ile
180 185 190
Gly Gly Gly Thr Pro Gln Pro Lys Asn Thr Ser Tyr Thr Phe Ser Ser
195 200 205
Ala Val Thr Ser Gly Leu Ser Thr Ser Asp Ala Ile Gly Gly Ser Leu
210 215 220
Thr Leu Gly Tyr Lys Tyr Ser Val Lys Glu Gly Gly Gly Val Leu Pro
225 230 235 240
Val Glu Ala Thr Gln Glu Phe Ser Leu Gln Leu Thr Ala Ser Tyr Asn
245 250 255
His Thr Ile Thr Val Ser Ser Gln Thr Thr Asn Thr Gln Thr Tyr Ser
260 265 270
Val Ala His Ala Gly Asp Ser Tyr Lys Asn Asp Lys Tyr Val Ala Ala
275 280 285
Met Tyr Gln Leu Lys Ser His Tyr Thr Val Ile Pro Gly Pro Ala Leu
290 295 300
Thr Gln Ser Gly Ser Ile Leu Ala Gln Glu Ala Phe Gln Tyr Asp Asp
305 310 315 320
Ser Ser Leu Tyr Leu Ala Val Thr Pro Gly Ala Gly Ile
325 330
<210> 60
<211> 324
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 60
Met Ala Pro Ile Met Ala Leu Ser Thr Ala Val Leu Leu Ser Pro Gly
1 5 10 15
Ser Thr Phe Ala Ala Glu Lys Ala Val Val Thr Lys Ser Asn Val Ser
20 25 30
Ser Leu Thr Thr Asn Thr Val Met Gln Ser Gly Ser Ile Ile Gln Gly
35 40 45
Tyr Leu Ile Lys Asn Gly Val Lys Thr Pro Val Tyr Asn Ser Glu Val
50 55 60
Gln Thr Arg Ser Thr Ala Val Asn Glu Ala Pro Tyr Pro Glu Leu Ser
65 70 75 80
Ser Asn Pro Asn Asp Pro Val Pro Ser Lys Gly Ser Ile Thr Ser Glu
85 90 95
Ser Gly Asn Val Gly Ser Val Leu Tyr Phe Ser Lys Phe Asn Ser Gln
100 105 110
Lys Leu Gln Asn Thr Ala Glu Pro Val Tyr Trp Lys Asn Val Tyr Leu
115 120 125
Glu Lys Thr Pro Asp Gly Asn Ile Ile Phe Gly Thr Tyr Asp Pro Thr
130 135 140
Thr Leu Lys Arg Thr Pro Asn Leu Val Asn Ile Met Met Thr Pro Ser
145 150 155 160
Lys Val Gln Tyr Tyr Gln Ser Phe Phe Thr Asp Thr Lys Ile Lys Arg
165 170 175
Glu Thr Ala Tyr Glu Lys Ile Gly Gly Gly Thr Pro Gln Pro Lys Asn
180 185 190
Thr Ser Tyr Thr Phe Ser Ser Ala Val Thr Ser Gly Leu Ser Thr Ser
195 200 205
Asp Ala Ile Gly Gly Ser Leu Thr Leu Gly Tyr Lys Tyr Ser Val Lys
210 215 220
Glu Gly Gly Gly Val Leu Pro Val Glu Ala Thr Gln Glu Phe Ser Leu
225 230 235 240
Gln Leu Thr Ala Ser Tyr Asn His Thr Ile Thr Val Ser Ser Gln Thr
245 250 255
Thr Asn Thr Gln Thr Tyr Ser Val Ala His Ala Gly Asp Ser Tyr Lys
260 265 270
Asn Asp Lys Tyr Val Ala Ala Met Tyr Gln Leu Lys Ser His Tyr Thr
275 280 285
Val Ile Pro Gly Pro Ala Leu Thr Gln Ser Gly Ser Ile Leu Ala Gln
290 295 300
Glu Ala Phe Gln Tyr Asp Asp Ser Ser Leu Tyr Leu Ala Val Thr Pro
305 310 315 320
Gly Ala Gly Ile
<210> 61
<211> 320
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 61
Met Ala Leu Ser Thr Ala Val Leu Leu Ser Pro Gly Ser Thr Phe Ala
1 5 10 15
Ala Glu Lys Ala Val Val Thr Lys Ser Asn Val Ser Ser Leu Thr Thr
20 25 30
Asn Thr Val Met Gln Ser Gly Ser Ile Ile Gln Gly Tyr Leu Ile Lys
35 40 45
Asn Gly Val Lys Thr Pro Val Tyr Asn Ser Glu Val Gln Thr Arg Ser
50 55 60
Thr Ala Val Asn Glu Ala Pro Tyr Pro Glu Leu Ser Ser Asn Pro Asn
65 70 75 80
Asp Pro Val Pro Ser Lys Gly Ser Ile Thr Ser Glu Ser Gly Asn Val
85 90 95
Gly Ser Val Leu Tyr Phe Ser Lys Phe Asn Ser Gln Lys Leu Gln Asn
100 105 110
Thr Ala Glu Pro Val Tyr Trp Lys Asn Val Tyr Leu Glu Lys Thr Pro
115 120 125
Asp Gly Asn Ile Ile Phe Gly Thr Tyr Asp Pro Thr Thr Leu Lys Arg
130 135 140
Thr Pro Asn Leu Val Asn Ile Met Met Thr Pro Ser Lys Val Gln Tyr
145 150 155 160
Tyr Gln Ser Phe Phe Thr Asp Thr Lys Ile Lys Arg Glu Thr Ala Tyr
165 170 175
Glu Lys Ile Gly Gly Gly Thr Pro Gln Pro Lys Asn Thr Ser Tyr Thr
180 185 190
Phe Ser Ser Ala Val Thr Ser Gly Leu Ser Thr Ser Asp Ala Ile Gly
195 200 205
Gly Ser Leu Thr Leu Gly Tyr Lys Tyr Ser Val Lys Glu Gly Gly Gly
210 215 220
Val Leu Pro Val Glu Ala Thr Gln Glu Phe Ser Leu Gln Leu Thr Ala
225 230 235 240
Ser Tyr Asn His Thr Ile Thr Val Ser Ser Gln Thr Thr Asn Thr Gln
245 250 255
Thr Tyr Ser Val Ala His Ala Gly Asp Ser Tyr Lys Asn Asp Lys Tyr
260 265 270
Val Ala Ala Met Tyr Gln Leu Lys Ser His Tyr Thr Val Ile Pro Gly
275 280 285
Pro Ala Leu Thr Gln Ser Gly Ser Ile Leu Ala Gln Glu Ala Phe Gln
290 295 300
Tyr Asp Asp Ser Ser Leu Tyr Leu Ala Val Thr Pro Gly Ala Gly Ile
305 310 315 320
<210> 62
<211> 285
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 62
Met Gln Ser Gly Ser Ile Ile Gln Gly Tyr Leu Ile Lys Asn Gly Val
1 5 10 15
Lys Thr Pro Val Tyr Asn Ser Glu Val Gln Thr Arg Ser Thr Ala Val
20 25 30
Asn Glu Ala Pro Tyr Pro Glu Leu Ser Ser Asn Pro Asn Asp Pro Val
35 40 45
Pro Ser Lys Gly Ser Ile Thr Ser Glu Ser Gly Asn Val Gly Ser Val
50 55 60
Leu Tyr Phe Ser Lys Phe Asn Ser Gln Lys Leu Gln Asn Thr Ala Glu
65 70 75 80
Pro Val Tyr Trp Lys Asn Val Tyr Leu Glu Lys Thr Pro Asp Gly Asn
85 90 95
Ile Ile Phe Gly Thr Tyr Asp Pro Thr Thr Leu Lys Arg Thr Pro Asn
100 105 110
Leu Val Asn Ile Met Met Thr Pro Ser Lys Val Gln Tyr Tyr Gln Ser
115 120 125
Phe Phe Thr Asp Thr Lys Ile Lys Arg Glu Thr Ala Tyr Glu Lys Ile
130 135 140
Gly Gly Gly Thr Pro Gln Pro Lys Asn Thr Ser Tyr Thr Phe Ser Ser
145 150 155 160
Ala Val Thr Ser Gly Leu Ser Thr Ser Asp Ala Ile Gly Gly Ser Leu
165 170 175
Thr Leu Gly Tyr Lys Tyr Ser Val Lys Glu Gly Gly Gly Val Leu Pro
180 185 190
Val Glu Ala Thr Gln Glu Phe Ser Leu Gln Leu Thr Ala Ser Tyr Asn
195 200 205
His Thr Ile Thr Val Ser Ser Gln Thr Thr Asn Thr Gln Thr Tyr Ser
210 215 220
Val Ala His Ala Gly Asp Ser Tyr Lys Asn Asp Lys Tyr Val Ala Ala
225 230 235 240
Met Tyr Gln Leu Lys Ser His Tyr Thr Val Ile Pro Gly Pro Ala Leu
245 250 255
Thr Gln Ser Gly Ser Ile Leu Ala Gln Glu Ala Phe Gln Tyr Asp Asp
260 265 270
Ser Ser Leu Tyr Leu Ala Val Thr Pro Gly Ala Gly Ile
275 280 285
<210> 63
<211> 304
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 63
Met Glu Lys Ala Val Val Thr Lys Ser Asn Val Ser Ser Leu Thr Thr
1 5 10 15
Asn Thr Val Met Gln Ser Gly Ser Ile Ile Gln Gly Tyr Leu Ile Lys
20 25 30
Asn Gly Val Lys Thr Pro Val Tyr Asn Ser Glu Val Gln Thr Arg Ser
35 40 45
Thr Ala Val Asn Glu Ala Pro Tyr Pro Glu Leu Ser Ser Asn Pro Asn
50 55 60
Asp Pro Val Pro Ser Lys Gly Ser Ile Thr Ser Glu Ser Gly Asn Val
65 70 75 80
Gly Ser Val Leu Tyr Phe Ser Lys Phe Asn Ser Gln Lys Leu Gln Asn
85 90 95
Thr Ala Glu Pro Val Tyr Trp Lys Asn Val Tyr Leu Glu Lys Thr Pro
100 105 110
Asp Gly Asn Ile Ile Phe Gly Thr Tyr Asp Pro Thr Thr Leu Lys Arg
115 120 125
Thr Pro Asn Leu Val Asn Ile Met Met Thr Pro Ser Lys Val Gln Tyr
130 135 140
Tyr Gln Ser Phe Phe Thr Asp Thr Lys Ile Lys Arg Glu Thr Ala Tyr
145 150 155 160
Glu Lys Ile Gly Gly Gly Thr Pro Gln Pro Lys Asn Thr Ser Tyr Thr
165 170 175
Phe Ser Ser Ala Val Thr Ser Gly Leu Ser Thr Ser Asp Ala Ile Gly
180 185 190
Gly Ser Leu Thr Leu Gly Tyr Lys Tyr Ser Val Lys Glu Gly Gly Gly
195 200 205
Val Leu Pro Val Glu Ala Thr Gln Glu Phe Ser Leu Gln Leu Thr Ala
210 215 220
Ser Tyr Asn His Thr Ile Thr Val Ser Ser Gln Thr Thr Asn Thr Gln
225 230 235 240
Thr Tyr Ser Val Ala His Ala Gly Asp Ser Tyr Lys Asn Asp Lys Tyr
245 250 255
Val Ala Ala Met Tyr Gln Leu Lys Ser His Tyr Thr Val Ile Pro Gly
260 265 270
Pro Ala Leu Thr Gln Ser Gly Ser Ile Leu Ala Gln Glu Ala Phe Gln
275 280 285
Tyr Asp Asp Ser Ser Leu Tyr Leu Ala Val Thr Pro Gly Ala Gly Ile
290 295 300
<210> 64
<211> 285
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 64
Met Gln Ser Gly Ser Ile Ile Gln Gly Tyr Leu Ile Lys Asn Gly Val
1 5 10 15
Lys Thr Pro Val Tyr Asn Ser Glu Val Gln Thr Arg Ser Thr Ala Val
20 25 30
Asn Glu Ala Pro Tyr Pro Glu Leu Ser Ser Asn Pro Asn Asp Pro Val
35 40 45
Pro Ser Lys Gly Ser Ile Thr Ser Glu Ser Gly Asn Val Gly Ser Val
50 55 60
Leu Tyr Phe Ser Lys Phe Asn Ser Gln Lys Leu Gln Asn Thr Ala Glu
65 70 75 80
Pro Val Tyr Trp Lys Asn Val Tyr Leu Glu Lys Thr Pro Asp Gly Asn
85 90 95
Ile Ile Phe Gly Thr Tyr Asp Pro Thr Thr Leu Lys Arg Thr Pro Asn
100 105 110
Leu Val Asn Ile Met Met Thr Pro Ser Lys Val Gln Tyr Tyr Gln Ser
115 120 125
Phe Phe Thr Asp Thr Lys Ile Lys Arg Glu Thr Ala Tyr Glu Lys Ile
130 135 140
Gly Gly Gly Thr Pro Gln Pro Lys Asn Thr Ser Tyr Thr Phe Ser Ser
145 150 155 160
Ala Val Thr Ser Gly Leu Ser Thr Ser Asp Ala Ile Gly Gly Ser Leu
165 170 175
Thr Leu Gly Tyr Lys Tyr Ser Val Lys Glu Gly Gly Gly Val Leu Pro
180 185 190
Val Glu Ala Thr Gln Glu Phe Ser Leu Gln Leu Thr Ala Ser Tyr Asn
195 200 205
His Thr Ile Thr Val Ser Ser Gln Thr Thr Asn Thr Gln Thr Tyr Ser
210 215 220
Val Ala His Ala Gly Asp Ser Tyr Lys Asn Asp Lys Tyr Val Ala Ala
225 230 235 240
Met Tyr Gln Leu Lys Ser His Tyr Thr Val Ile Pro Gly Pro Ala Leu
245 250 255
Thr Gln Ser Gly Ser Ile Leu Ala Gln Glu Ala Phe Gln Tyr Asp Asp
260 265 270
Ser Ser Leu Tyr Leu Ala Val Thr Pro Gly Ala Gly Ile
275 280 285
<210> 65
<211> 385
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 65
Met Ala Ile Ile Asn Gln Ser Ser Leu Asn Ser Arg Ile His Asp Leu
1 5 10 15
Arg Glu Asp Ser Arg Thr Ala Leu Glu Lys Val Tyr Thr Ser Asn Asn
20 25 30
Pro Trp Gly Phe Val Ser Ile His Ser Asp Arg Leu Glu Asn Tyr Gln
35 40 45
Leu Thr Asn Val Asn Val Ser Pro Arg Asn Gln Asp Phe Gln Thr Ile
50 55 60
Pro Arg Leu Gln His Ser Ala Thr Gln Ile Ile Glu Asn Asn Thr Ser
65 70 75 80
Val Thr Gln Ser Gln Thr Ile Ser Phe Asn Glu Arg Thr Thr Asp Thr
85 90 95
Phe Thr Thr Ser Val Thr Thr Gly Phe Lys Thr Gly Thr Ser Val Lys
100 105 110
Ser Thr Thr Lys Phe Lys Ile Ser Val Gly Phe Leu Leu Ala Gly Glu
115 120 125
Leu Glu Gln Ser Val Glu Val Ser Val Asn Phe Glu Tyr Asn Tyr Ser
130 135 140
Ser Thr Thr Thr Glu Thr His Ser Val Glu Arg Gly Trp Thr Ile Ser
145 150 155 160
Gln Pro Ile Ile Ala Pro Pro Arg Thr Arg Val Glu Ala Thr Leu Leu
165 170 175
Ile Tyr Ala Gly Ser Val Asp Val Pro Ile Asp Leu Asn Ala Thr Ile
180 185 190
Val Gly Asp Pro Ile Pro Trp Pro Ser Trp Gly Pro Ala Val Tyr Ser
195 200 205
Gly Ser Phe Leu Ala Asn Asp Gly Arg Ile Trp Ser Ala Pro Ile Leu
210 215 220
Pro Glu Gln Leu Ser Leu Ala Ser Ser Ala Tyr Thr Thr Val Gly Arg
225 230 235 240
Thr Ala Asn Phe Ser Gly Leu Ala Thr Thr Asn Val Ser Ser Gly Leu
245 250 255
Tyr Ser Ile Val Arg Ile Asp Glu Ser Pro Leu Pro Gly Phe Thr Gly
260 265 270
Glu Thr Arg Arg Tyr Tyr Leu Pro Pro Ser Leu Ala Thr Thr Asn Gln
275 280 285
Ile Leu Ser Thr Asn Ala Leu Gly Asn Asn Val Pro Ile Ile Asn Pro
290 295 300
Val Pro Asn Gly His Cys Lys Lys Asp His Ser Pro Ile Ile Ile His
305 310 315 320
Lys Asn Arg Glu Val Lys Cys Glu His Asn Tyr Asp Glu Val Tyr Pro
325 330 335
Arg His Asp Gln Val Glu Lys Cys Glu His Asn Tyr Asp Glu Val Tyr
340 345 350
Pro Arg His Asp Gln Val Glu Lys Cys Glu His Asp Tyr Asp Glu Val
355 360 365
Tyr Pro Arg His Asp Gln Val Glu Lys Tyr Glu His Asn Tyr Asp Glu
370 375 380
Glu
385
<210> 66
<211> 334
<212> PRT
<213> 未知
<220>
<223> 从土壤样品中分离的
<400> 66
Met Ala Ile Ile Asn Gln Ser Ser Leu Asn Ser Arg Ile His Asp Leu
1 5 10 15
Arg Glu Asp Ser Arg Thr Ala Leu Glu Lys Val Tyr Thr Ser Asn Asn
20 25 30
Pro Trp Gly Phe Val Ser Ile His Ser Asp Arg Leu Glu Asn Tyr Gln
35 40 45
Leu Thr Asn Val Asn Val Ser Pro Arg Asn Gln Asp Phe Gln Thr Ile
50 55 60
Pro Arg Leu Gln His Ser Ala Thr Gln Ile Ile Glu Asn Asn Thr Ser
65 70 75 80
Val Thr Gln Ser Gln Thr Ile Ser Phe Asn Glu Arg Thr Thr Asp Thr
85 90 95
Phe Thr Thr Ser Val Thr Thr Gly Phe Lys Thr Gly Thr Ser Val Lys
100 105 110
Ser Thr Thr Lys Phe Lys Ile Ser Val Gly Phe Leu Leu Ala Gly Glu
115 120 125
Leu Glu Gln Ser Val Glu Val Ser Val Asn Phe Glu Tyr Asn Tyr Ser
130 135 140
Ser Thr Thr Thr Glu Thr His Ser Val Glu Arg Gly Trp Thr Ile Ser
145 150 155 160
Gln Pro Ile Ile Ala Pro Pro Arg Thr Arg Val Glu Ala Thr Leu Leu
165 170 175
Ile Tyr Ala Gly Ser Val Asp Val Pro Ile Asp Leu Asn Ala Thr Ile
180 185 190
Val Gly Asp Pro Ile Pro Trp Pro Ser Trp Gly Pro Ala Val Tyr Ser
195 200 205
Gly Ser Phe Leu Ala Asn Asp Gly Arg Ile Trp Ser Ala Pro Ile Leu
210 215 220
Pro Glu Gln Leu Ser Leu Ala Ser Ser Ala Tyr Thr Thr Val Gly Arg
225 230 235 240
Thr Ala Asn Phe Ser Gly Leu Ala Thr Thr Asn Val Ser Ser Gly Leu
245 250 255
Tyr Ser Ile Val Arg Ile Asp Glu Ser Pro Leu Pro Gly Phe Thr Gly
260 265 270
Glu Thr Arg Arg Tyr Tyr Leu Pro Pro Ser Leu Ala Thr Thr Asn Gln
275 280 285
Ile Leu Ser Thr Asn Ala Leu Gly Asn Asn Val Pro Ile Ile Asn Pro
290 295 300
Val Pro Asn Gly His Cys Lys Lys Asp His Ser Pro Ile Ile Ile His
305 310 315 320
Lys Asn Arg Glu Val Lys Cys Glu His Asn Tyr Asp Glu Glu
325 330
<210> 67
<211> 1232
<212> PRT
<213> 苏云金芽孢杆菌
<400> 67
Met Asn Lys Asn Asn Gln Asn Glu Tyr Glu Ile Ile Asp Ala Ser Asn
1 5 10 15
Cys Gly Cys Ala Ser Asp Asp Val Ala Arg Tyr Pro Leu Ala Asn Asn
20 25 30
Pro Tyr Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile
35 40 45
Leu Asn Trp Ile Asn Ile Ile Gly Asn Ala Ala Lys Glu Ala Val Ser
50 55 60
Ile Gly Leu Thr Ile Lys Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly
65 70 75 80
Leu Ile Ser Ile Ala Tyr Asn Leu Leu Gly Lys Val Leu Gly Gly Ser
85 90 95
Ser Gly Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile
100 105 110
Ile Asp Leu Arg Val Asn Gln Ser Val Leu Asn Asp Gly Ile Ala Asp
115 120 125
Phe Asn Gly Ser Leu Ile Leu Tyr Arg Asn Tyr Leu Asp Ala Leu Asn
130 135 140
Ser Trp Asn Glu Asn Pro Asn Ser Asn Arg Ala Glu Glu Leu Arg Ala
145 150 155 160
Arg Phe Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly
165 170 175
Ser Leu Thr Asn Gly Gly Ser Leu Ala Arg Gln Asp Ala Gln Ile Leu
180 185 190
Leu Leu Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu
195 200 205
Arg Asp Ala Ala Arg Tyr Gly Asn Asp Trp Asp Leu Phe Gly Ala Ile
210 215 220
Pro Phe Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr
225 230 235 240
Thr Asp Tyr Cys Val Asn Trp Tyr Asn Gln Gly Phe Asn Glu Leu Arg
245 250 255
Gln Arg Gly Thr Ser Ala Thr Val Trp Leu Glu Phe His Arg Tyr Arg
260 265 270
Arg Glu Met Thr Leu Thr Val Leu Asp Ile Val Ala Ser Phe Ser Ser
275 280 285
Leu Asp Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg
290 295 300
Ile Ile Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg
305 310 315 320
Gly Glu Ser Trp Phe Ser Phe Ile Asn Arg Ala Asn Phe Ser Glu Leu
325 330 335
Glu Asn Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met
340 345 350
Ile Ile Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Asn Thr Asp
355 360 365
Arg Ala Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn
370 375 380
Ser Gln Val Ile Ser Glu Leu Ile Ser Gly Gln His Thr Asn Ser Thr
385 390 395 400
Gln Thr Ile Leu Gly Arg Asn Ile Phe Arg Ile Asp Ser Gln Ala Cys
405 410 415
Asn Leu Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His
420 425 430
Asp Ala Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Phe Ile Arg
435 440 445
Thr Thr Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Phe
450 455 460
Pro Gly Glu Asn Ser Asn Ile Pro Thr Pro Glu Asp Tyr Thr His Leu
465 470 475 480
Leu Ser Thr Thr Val Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Asn
485 490 495
Asn Arg Arg Ser Ser Ile Val Ile Tyr Gly Trp Thr His Lys Ser Leu
500 505 510
Thr Arg Asn Asn Thr Ile Asn Pro Gly Ile Ile Thr Gln Ile Pro Met
515 520 525
Val Lys Leu Ser Asn Leu Ser Ser Gly Thr Asn Val Val Arg Gly Pro
530 535 540
Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr Asn Ala Gly Asn Phe
545 550 555 560
Gly Asp Val Arg Val Asn Ile Ala Gly Ser Leu Ser Gln Arg Tyr Arg
565 570 575
Val Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser
580 585 590
Ile Asn Gly Arg Ala Ile Asn Gln Ala Asn Phe Pro Ala Thr Met Asn
595 600 605
Ile Gly Ala Ser Leu Asn Tyr Arg Thr Phe Arg Thr Val Gly Phe Thr
610 615 620
Thr Pro Phe Thr Phe Ser Glu Ala Ser Ser Ile Phe Thr Leu Ser Thr
625 630 635 640
His Ser Phe Ser Ser Gly Asn Ala Val Tyr Ile Asp Arg Ile Glu Phe
645 650 655
Val Pro Ala Glu Val Thr Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala
660 665 670
Gln Lys Ala Val Asn Ala Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu
675 680 685
Lys Thr Asp Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val
690 695 700
Ala Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser
705 710 715 720
Glu Lys Val Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu
725 730 735
Gln Asp Pro Asn Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp
740 745 750
Arg Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys
755 760 765
Glu Asn Tyr Val Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr
770 775 780
Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg
785 790 795 800
Tyr Glu Leu Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Val Tyr
805 810 815
Leu Ile Arg Tyr Asn Ala Lys His Glu Thr Leu Asn Val Pro Gly Thr
820 825 830
Gly Ser Leu Trp Pro Leu Ala Ala Glu Ser Ser Ile Gly Arg Cys Gly
835 840 845
Glu Pro Asn Arg Cys Ala Pro His Ile Glu Trp Asn Pro Asp Leu Asp
850 855 860
Cys Ser Cys Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe
865 870 875 880
Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly
885 890 895
Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu
900 905 910
Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu
915 920 925
Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Asp Lys
930 935 940
Leu Glu Leu Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val
945 950 955 960
Asp Ala Leu Phe Val Asp Ser Gln Tyr Asn Arg Leu Gln Thr Asp Thr
965 970 975
Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg
980 985 990
Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala
995 1000 1005
Ile Phe Glu Glu Leu Glu Gly Leu Ile Phe Thr Ala Phe Ser Leu
1010 1015 1020
Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn His Gly
1025 1030 1035
Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln
1040 1045 1050
Asn Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu
1055 1060 1065
Val Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu
1070 1075 1080
Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr
1085 1090 1095
Ile His Glu Ile Glu Asp His Thr Asp Glu Leu Lys Phe Arg Asn
1100 1105 1110
Cys Glu Glu Glu Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn
1115 1120 1125
Asp Tyr Pro Ala Asn Gln Glu Glu Tyr Lys Gly Ala Tyr Pro Ser
1130 1135 1140
Arg Asn Gly Gly Tyr Glu Asp Thr Tyr Asp Thr Ser Ala Ser Val
1145 1150 1155
His Tyr Asn Thr Pro Thr Tyr Glu Glu Glu Ile Gly Thr Asp Leu
1160 1165 1170
Gln Arg Tyr Asn Gln Cys Glu Asn Asn Arg Gly Tyr Gly Asn Tyr
1175 1180 1185
Thr Pro Leu Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe
1190 1195 1200
Pro Glu Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly
1205 1210 1215
Thr Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu
1220 1225 1230
<210> 68
<211> 659
<212> PRT
<213> 苏云金芽孢杆菌
<400> 68
Met Asn Lys Asn Asn Gln Asn Glu Tyr Glu Ile Ile Asp Ala Ser Asn
1 5 10 15
Cys Gly Cys Ala Ser Asp Asp Val Ala Arg Tyr Pro Leu Ala Asn Asn
20 25 30
Pro Tyr Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile
35 40 45
Leu Asn Trp Ile Asn Ile Ile Gly Asn Ala Ala Lys Glu Ala Val Ser
50 55 60
Ile Gly Leu Thr Ile Lys Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly
65 70 75 80
Leu Ile Ser Ile Ala Tyr Asn Leu Leu Gly Lys Val Leu Gly Gly Ser
85 90 95
Ser Gly Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile
100 105 110
Ile Asp Leu Arg Val Asn Gln Ser Val Leu Asn Asp Gly Ile Ala Asp
115 120 125
Phe Asn Gly Ser Leu Ile Leu Tyr Arg Asn Tyr Leu Asp Ala Leu Asn
130 135 140
Ser Trp Asn Glu Asn Pro Asn Ser Asn Arg Ala Glu Glu Leu Arg Ala
145 150 155 160
Arg Phe Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly
165 170 175
Ser Leu Thr Asn Gly Gly Ser Leu Ala Arg Gln Asp Ala Gln Ile Leu
180 185 190
Leu Leu Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu
195 200 205
Arg Asp Ala Ala Arg Tyr Gly Asn Asp Trp Asp Leu Phe Gly Ala Ile
210 215 220
Pro Phe Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr
225 230 235 240
Thr Asp Tyr Cys Val Asn Trp Tyr Asn Gln Gly Phe Asn Glu Leu Arg
245 250 255
Gln Arg Gly Thr Ser Ala Thr Val Trp Leu Glu Phe His Arg Tyr Arg
260 265 270
Arg Glu Met Thr Leu Thr Val Leu Asp Ile Val Ala Ser Phe Ser Ser
275 280 285
Leu Asp Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg
290 295 300
Ile Ile Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg
305 310 315 320
Gly Glu Ser Trp Phe Ser Phe Ile Asn Arg Ala Asn Phe Ser Glu Leu
325 330 335
Glu Asn Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met
340 345 350
Ile Ile Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Asn Thr Asp
355 360 365
Arg Ala Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn
370 375 380
Ser Gln Val Ile Ser Glu Leu Ile Ser Gly Gln His Thr Asn Ser Thr
385 390 395 400
Gln Thr Ile Leu Gly Arg Asn Ile Phe Arg Ile Asp Ser Gln Ala Cys
405 410 415
Asn Leu Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His
420 425 430
Asp Ala Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Phe Ile Arg
435 440 445
Thr Thr Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Phe
450 455 460
Pro Gly Glu Asn Ser Asn Ile Pro Thr Pro Glu Asp Tyr Thr His Leu
465 470 475 480
Leu Ser Thr Thr Val Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Asn
485 490 495
Asn Arg Arg Ser Ser Ile Val Ile Tyr Gly Trp Thr His Lys Ser Leu
500 505 510
Thr Arg Asn Asn Thr Ile Asn Pro Gly Ile Ile Thr Gln Ile Pro Met
515 520 525
Val Lys Leu Ser Asn Leu Ser Ser Gly Thr Asn Val Val Arg Gly Pro
530 535 540
Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr Asn Ala Gly Asn Phe
545 550 555 560
Gly Asp Val Arg Val Asn Ile Ala Gly Ser Leu Ser Gln Arg Tyr Arg
565 570 575
Val Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser
580 585 590
Ile Asn Gly Arg Ala Ile Asn Gln Ala Asn Phe Pro Ala Thr Met Asn
595 600 605
Ile Gly Ala Ser Leu Asn Tyr Arg Thr Phe Arg Thr Val Gly Phe Thr
610 615 620
Thr Pro Phe Thr Phe Ser Glu Ala Ser Ser Ile Phe Thr Leu Ser Thr
625 630 635 640
His Ser Phe Ser Ser Gly Asn Ala Val Tyr Ile Asp Arg Ile Glu Phe
645 650 655
Val Pro Ala
<210> 69
<211> 1233
<212> PRT
<213> 苏云金芽孢杆菌
<400> 69
Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser
1 5 10 15
Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asp Leu Ser Pro Asp
20 25 30
Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp
35 40 45
Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly
50 55 60
Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Leu Ala Ser
65 70 75 80
Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Ser Gly Arg Asp Pro
85 90 95
Trp Glu Ile Phe Met Glu His Val Glu Gln Leu Val Arg Gln Gln Ile
100 105 110
Thr Asp Ser Val Arg Asp Thr Ala Ile Ala Arg Leu Glu Gly Leu Gly
115 120 125
Arg Gly Tyr Arg Ser Tyr Gln Gln Ala Leu Glu Thr Trp Leu Asp Asn
130 135 140
Arg Asn Asp Ala Arg Ser Arg Ser Ile Ile Leu Glu Arg Tyr Ile Ala
145 150 155 160
Leu Glu Leu Asp Ile Thr Thr Ala Ile Pro Leu Phe Ser Ile Arg Asn
165 170 175
Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His
180 185 190
Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Trp Gly Met
195 200 205
Ser Ser Ala Asp Val Asn Gln Tyr Tyr Gln Glu Gln Ile Arg Tyr Thr
210 215 220
Glu Glu Tyr Ser Asn His Cys Val Gln Trp Tyr Asn Thr Gly Leu Asn
225 230 235 240
Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Val Arg Tyr Asn Gln Phe
245 250 255
Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro
260 265 270
Ser Tyr Asp Thr Arg Thr Tyr Pro Ile Asn Thr Ser Ala Gln Leu Thr
275 280 285
Arg Glu Val Tyr Thr Asp Ala Ile Gly Ala Thr Gly Val Asn Met Ala
290 295 300
Asn Met Asn Trp Tyr Asn Asn Asn Ala Pro Ser Phe Ser Ala Ile Glu
305 310 315 320
Ala Ala Val Ile Arg Ser Pro His Leu Leu Asp Phe Leu Glu Gln Leu
325 330 335
Thr Ile Phe Ser Ala Ser Ser Arg Trp Ser Asn Thr Arg His Met Thr
340 345 350
Tyr Trp Arg Gly His Thr Ile Gln Ser Arg Pro Ile Gly Gly Gly Leu
355 360 365
Asn Thr Ser Thr Tyr Gly Ser Thr Asn Thr Ser Ile Asn Pro Val Thr
370 375 380
Leu Arg Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Trp Ala Gly
385 390 395 400
Val Leu Leu Trp Gly Ile Tyr Leu Glu Pro Ile His Gly Val Pro Thr
405 410 415
Val Arg Phe Asn Phe Thr Asn Pro Gln Asn Ile Tyr Asp Arg Gly Thr
420 425 430
Ala Asn Tyr Ser Gln Pro Tyr Glu Ser Pro Gly Leu Gln Leu Lys Asp
435 440 445
Ser Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu
450 455 460
Ser Tyr Ser His Arg Leu Ser His Ile Gly Ile Ile Leu Gln Ser Arg
465 470 475 480
Val Asn Val Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr
485 490 495
Asn Thr Ile Gly Pro Asn Arg Ile Thr Gln Ile Pro Met Val Lys Ala
500 505 510
Ser Glu Leu Pro Gln Gly Thr Thr Val Val Arg Gly Pro Gly Phe Thr
515 520 525
Gly Gly Asp Ile Leu Arg Arg Thr Asn Thr Gly Gly Phe Gly Pro Ile
530 535 540
Arg Val Thr Val Asn Gly Pro Leu Thr Gln Arg Tyr Arg Ile Gly Phe
545 550 555 560
Arg Tyr Ala Ser Thr Val Asp Phe Asp Phe Phe Val Ser Arg Gly Gly
565 570 575
Thr Thr Val Asn Asn Phe Arg Phe Leu Arg Thr Met Asn Ser Gly Asp
580 585 590
Glu Leu Lys Tyr Gly Asn Phe Val Arg Arg Ala Phe Thr Thr Pro Phe
595 600 605
Thr Phe Thr Gln Ile Gln Asp Ile Ile Arg Thr Ser Ile Gln Gly Leu
610 615 620
Ser Gly Asn Gly Glu Val Tyr Ile Asp Lys Ile Glu Ile Ile Pro Val
625 630 635 640
Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Glu Ala
645 650 655
Val Asn Ala Leu Phe Thr Asn Thr Asn Pro Arg Arg Leu Lys Thr Asp
660 665 670
Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu
675 680 685
Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Leu Glu Lys Val
690 695 700
Lys Tyr Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro
705 710 715 720
Asn Phe Thr Ser Ile Asn Lys Gln Pro Asp Phe Ile Ser Thr Asn Glu
725 730 735
Gln Ser Asn Phe Thr Ser Ile His Glu Gln Ser Glu His Gly Trp Trp
740 745 750
Gly Ser Glu Asn Ile Thr Ile Gln Glu Gly Asn Asp Val Phe Lys Glu
755 760 765
Asn Tyr Val Thr Leu Pro Gly Thr Phe Asn Glu Cys Tyr Pro Thr Tyr
770 775 780
Leu Tyr Gln Lys Ile Gly Glu Ser Glu Leu Lys Ala Tyr Thr Arg Tyr
785 790 795 800
Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu
805 810 815
Ile Arg Tyr Asn Ala Lys Pro Glu Thr Leu Asp Val Pro Gly Thr Glu
820 825 830
Ser Leu Trp Pro Leu Ser Val Glu Ser Pro Ile Gly Arg Cys Gly Glu
835 840 845
Pro Asn Arg Cys Ala Pro His Phe Glu Trp Asn Pro Asp Leu Asp Cys
850 855 860
Ser Cys Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser
865 870 875 880
Leu Asp Ile Asp Val Gly Cys Thr Asp Leu His Glu Asn Leu Gly Val
885 890 895
Trp Val Val Phe Lys Ile Lys Thr Gln Glu Gly His Ala Arg Leu Gly
900 905 910
Asn Leu Glu Phe Ile Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ser
915 920 925
Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu
930 935 940
Gln Leu Glu Thr Lys Arg Val Tyr Thr Glu Ala Lys Glu Ala Val Asp
945 950 955 960
Ala Leu Phe Val Asp Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn
965 970 975
Ile Gly Met Ile His Ala Ala Asp Lys Leu Val His Arg Ile Arg Glu
980 985 990
Ala Tyr Leu Ser Glu Leu Pro Val Ile Pro Gly Val Asn Ala Glu Ile
995 1000 1005
Phe Glu Glu Leu Glu Gly His Ile Ile Thr Ala Ile Ser Leu Tyr
1010 1015 1020
Asp Ala Arg Asn Val Val Lys Asn Gly Asp Phe Asn Asn Gly Leu
1025 1030 1035
Thr Cys Trp Asn Val Lys Gly His Val Asp Val Gln Gln Ser His
1040 1045 1050
His Arg Ser Asp Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser
1055 1060 1065
Gln Ala Val Arg Val Cys Pro Gly Cys Gly Tyr Ile Leu Arg Val
1070 1075 1080
Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His
1085 1090 1095
Glu Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Lys Asn Arg Glu
1100 1105 1110
Glu Glu Glu Val Tyr Pro Thr Asp Thr Gly Thr Cys Asn Asp Tyr
1115 1120 1125
Thr Ala His Gln Gly Thr Ala Gly Cys Ala Asp Ala Cys Asn Ser
1130 1135 1140
Arg Asn Ala Gly Tyr Glu Asp Ala Tyr Glu Val Asp Thr Thr Ala
1145 1150 1155
Ser Val Asn Tyr Lys Pro Thr Tyr Glu Glu Glu Thr Tyr Thr Asp
1160 1165 1170
Val Arg Arg Asp Asn His Cys Glu Tyr Asp Arg Gly Tyr Val Asn
1175 1180 1185
Tyr Pro Pro Val Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr
1190 1195 1200
Phe Pro Glu Thr Asp Thr Val Trp Ile Glu Ile Gly Glu Thr Glu
1205 1210 1215
Gly Lys Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu
1220 1225 1230
<210> 70
<211> 1207
<212> PRT
<213> 苏云金芽孢杆菌
<400> 70
Met Asp Leu Ser Pro Asp Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala
1 5 10 15
Glu Gly Asn Asn Ile Asp Pro Phe Val Ser Ala Ser Thr Val Gln Thr
20 25 30
Gly Ile Asn Ile Ala Gly Arg Ile Leu Gly Val Leu Gly Val Pro Phe
35 40 45
Ala Gly Gln Leu Ala Ser Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp
50 55 60
Pro Ser Gly Arg Asp Pro Trp Glu Ile Phe Met Glu His Val Glu Gln
65 70 75 80
Leu Val Arg Gln Gln Ile Thr Asp Ser Val Arg Asp Thr Ala Ile Ala
85 90 95
Arg Leu Glu Gly Leu Gly Arg Gly Tyr Arg Ser Tyr Gln Gln Ala Leu
100 105 110
Glu Thr Trp Leu Asp Asn Arg Asn Asp Ala Arg Ser Arg Ser Ile Ile
115 120 125
Leu Glu Arg Tyr Ile Ala Leu Glu Leu Asp Ile Thr Thr Ala Ile Pro
130 135 140
Leu Phe Ser Ile Arg Asn Gln Glu Val Pro Leu Leu Met Val Tyr Ala
145 150 155 160
Gln Ala Ala Asn Leu His Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe
165 170 175
Gly Ser Glu Trp Gly Met Ser Ser Ala Asp Val Asn Gln Tyr Tyr Gln
180 185 190
Glu Gln Ile Arg Tyr Thr Glu Glu Tyr Ser Asn His Cys Val Gln Trp
195 200 205
Tyr Asn Thr Gly Leu Asn Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp
210 215 220
Val Arg Tyr Asn Gln Phe Arg Arg Asp Leu Thr Leu Gly Val Leu Asp
225 230 235 240
Leu Val Ala Leu Phe Pro Ser Tyr Asp Thr Arg Thr Tyr Pro Ile Asn
245 250 255
Thr Ser Ala Gln Leu Thr Arg Glu Val Tyr Thr Asp Ala Ile Gly Ala
260 265 270
Thr Gly Val Asn Met Ala Asn Met Asn Trp Tyr Asn Asn Asn Ala Pro
275 280 285
Ser Phe Ser Ala Ile Glu Ala Ala Val Ile Arg Ser Pro His Leu Leu
290 295 300
Asp Phe Leu Glu Gln Leu Thr Ile Phe Ser Ala Ser Ser Arg Trp Ser
305 310 315 320
Asn Thr Arg His Met Thr Tyr Trp Arg Gly His Thr Ile Gln Ser Arg
325 330 335
Pro Ile Gly Gly Gly Leu Asn Thr Ser Thr Tyr Gly Ser Thr Asn Thr
340 345 350
Ser Ile Asn Pro Val Thr Leu Arg Phe Thr Ser Arg Asp Val Tyr Arg
355 360 365
Thr Glu Ser Trp Ala Gly Val Leu Leu Trp Gly Ile Tyr Leu Glu Pro
370 375 380
Ile His Gly Val Pro Thr Val Arg Phe Asn Phe Thr Asn Pro Gln Asn
385 390 395 400
Ile Tyr Asp Arg Gly Thr Ala Asn Tyr Ser Gln Pro Tyr Glu Ser Pro
405 410 415
Gly Leu Gln Leu Lys Asp Ser Glu Thr Glu Leu Pro Pro Glu Thr Thr
420 425 430
Glu Arg Pro Asn Tyr Glu Ser Tyr Ser His Arg Leu Ser His Ile Gly
435 440 445
Ile Ile Leu Gln Ser Arg Val Asn Val Pro Val Tyr Ser Trp Thr His
450 455 460
Arg Ser Ala Asp Arg Thr Asn Thr Ile Gly Pro Asn Arg Ile Thr Gln
465 470 475 480
Ile Pro Met Val Lys Ala Ser Glu Leu Pro Gln Gly Thr Thr Val Val
485 490 495
Arg Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr Asn Thr
500 505 510
Gly Gly Phe Gly Pro Ile Arg Val Thr Val Asn Gly Pro Leu Thr Gln
515 520 525
Arg Tyr Arg Ile Gly Phe Arg Tyr Ala Ser Thr Val Asp Phe Asp Phe
530 535 540
Phe Val Ser Arg Gly Gly Thr Thr Val Asn Asn Phe Arg Phe Leu Arg
545 550 555 560
Thr Met Asn Ser Gly Asp Glu Leu Lys Tyr Gly Asn Phe Val Arg Arg
565 570 575
Ala Phe Thr Thr Pro Phe Thr Phe Thr Gln Ile Gln Asp Ile Ile Arg
580 585 590
Thr Ser Ile Gln Gly Leu Ser Gly Asn Gly Glu Val Tyr Ile Asp Lys
595 600 605
Ile Glu Ile Ile Pro Val Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu
610 615 620
Glu Arg Ala Gln Glu Ala Val Asn Ala Leu Phe Thr Asn Thr Asn Pro
625 630 635 640
Arg Arg Leu Lys Thr Asp Val Thr Asp Tyr His Ile Asp Gln Val Ser
645 650 655
Asn Leu Val Ala Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg
660 665 670
Glu Leu Leu Glu Lys Val Lys Tyr Ala Lys Arg Leu Ser Asp Glu Arg
675 680 685
Asn Leu Leu Gln Asp Pro Asn Phe Thr Ser Ile Asn Lys Gln Pro Asp
690 695 700
Phe Ile Ser Thr Asn Glu Gln Ser Asn Phe Thr Ser Ile His Glu Gln
705 710 715 720
Ser Glu His Gly Trp Trp Gly Ser Glu Asn Ile Thr Ile Gln Glu Gly
725 730 735
Asn Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Pro Gly Thr Phe Asn
740 745 750
Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Gly Glu Ser Glu Leu
755 760 765
Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln
770 775 780
Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys Pro Glu Thr Leu
785 790 795 800
Asp Val Pro Gly Thr Glu Ser Leu Trp Pro Leu Ser Val Glu Ser Pro
805 810 815
Ile Gly Arg Cys Gly Glu Pro Asn Arg Cys Ala Pro His Phe Glu Trp
820 825 830
Asn Pro Asp Leu Asp Cys Ser Cys Arg Asp Gly Glu Lys Cys Ala His
835 840 845
His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu
850 855 860
His Glu Asn Leu Gly Val Trp Val Val Phe Lys Ile Lys Thr Gln Glu
865 870 875 880
Gly His Ala Arg Leu Gly Asn Leu Glu Phe Ile Glu Glu Lys Pro Leu
885 890 895
Leu Gly Glu Ala Leu Ser Arg Val Lys Arg Ala Glu Lys Lys Trp Arg
900 905 910
Asp Lys Arg Glu Lys Leu Gln Leu Glu Thr Lys Arg Val Tyr Thr Glu
915 920 925
Ala Lys Glu Ala Val Asp Ala Leu Phe Val Asp Ser Gln Tyr Asp Arg
930 935 940
Leu Gln Ala Asp Thr Asn Ile Gly Met Ile His Ala Ala Asp Lys Leu
945 950 955 960
Val His Arg Ile Arg Glu Ala Tyr Leu Ser Glu Leu Pro Val Ile Pro
965 970 975
Gly Val Asn Ala Glu Ile Phe Glu Glu Leu Glu Gly His Ile Ile Thr
980 985 990
Ala Ile Ser Leu Tyr Asp Ala Arg Asn Val Val Lys Asn Gly Asp Phe
995 1000 1005
Asn Asn Gly Leu Thr Cys Trp Asn Val Lys Gly His Val Asp Val
1010 1015 1020
Gln Gln Ser His His Arg Ser Asp Leu Val Ile Pro Glu Trp Glu
1025 1030 1035
Ala Glu Val Ser Gln Ala Val Arg Val Cys Pro Gly Cys Gly Tyr
1040 1045 1050
Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys
1055 1060 1065
Val Thr Ile His Glu Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe
1070 1075 1080
Lys Asn Arg Glu Glu Glu Glu Val Tyr Pro Thr Asp Thr Gly Thr
1085 1090 1095
Cys Asn Asp Tyr Thr Ala His Gln Gly Thr Ala Gly Cys Ala Asp
1100 1105 1110
Ala Cys Asn Ser Arg Asn Ala Gly Tyr Glu Asp Ala Tyr Glu Val
1115 1120 1125
Asp Thr Thr Ala Ser Val Asn Tyr Lys Pro Thr Tyr Glu Glu Glu
1130 1135 1140
Thr Tyr Thr Asp Val Arg Arg Asp Asn His Cys Glu Tyr Asp Arg
1145 1150 1155
Gly Tyr Val Asn Tyr Pro Pro Val Pro Ala Gly Tyr Val Thr Lys
1160 1165 1170
Glu Leu Glu Tyr Phe Pro Glu Thr Asp Thr Val Trp Ile Glu Ile
1175 1180 1185
Gly Glu Thr Glu Gly Lys Phe Ile Val Asp Ser Val Glu Leu Leu
1190 1195 1200
Leu Met Glu Glu
1205
<210> 71
<211> 1133
<212> PRT
<213> 苏云金芽孢杆菌
<400> 71
Met Glu His Val Glu Gln Leu Val Arg Gln Gln Ile Thr Asp Ser Val
1 5 10 15
Arg Asp Thr Ala Ile Ala Arg Leu Glu Gly Leu Gly Arg Gly Tyr Arg
20 25 30
Ser Tyr Gln Gln Ala Leu Glu Thr Trp Leu Asp Asn Arg Asn Asp Ala
35 40 45
Arg Ser Arg Ser Ile Ile Leu Glu Arg Tyr Ile Ala Leu Glu Leu Asp
50 55 60
Ile Thr Thr Ala Ile Pro Leu Phe Ser Ile Arg Asn Gln Glu Val Pro
65 70 75 80
Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His Leu Leu Leu Leu
85 90 95
Arg Asp Ala Ser Leu Phe Gly Ser Glu Trp Gly Met Ser Ser Ala Asp
100 105 110
Val Asn Gln Tyr Tyr Gln Glu Gln Ile Arg Tyr Thr Glu Glu Tyr Ser
115 120 125
Asn His Cys Val Gln Trp Tyr Asn Thr Gly Leu Asn Asn Leu Arg Gly
130 135 140
Thr Asn Ala Glu Ser Trp Val Arg Tyr Asn Gln Phe Arg Arg Asp Leu
145 150 155 160
Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro Ser Tyr Asp Thr
165 170 175
Arg Thr Tyr Pro Ile Asn Thr Ser Ala Gln Leu Thr Arg Glu Val Tyr
180 185 190
Thr Asp Ala Ile Gly Ala Thr Gly Val Asn Met Ala Asn Met Asn Trp
195 200 205
Tyr Asn Asn Asn Ala Pro Ser Phe Ser Ala Ile Glu Ala Ala Val Ile
210 215 220
Arg Ser Pro His Leu Leu Asp Phe Leu Glu Gln Leu Thr Ile Phe Ser
225 230 235 240
Ala Ser Ser Arg Trp Ser Asn Thr Arg His Met Thr Tyr Trp Arg Gly
245 250 255
His Thr Ile Gln Ser Arg Pro Ile Gly Gly Gly Leu Asn Thr Ser Thr
260 265 270
Tyr Gly Ser Thr Asn Thr Ser Ile Asn Pro Val Thr Leu Arg Phe Thr
275 280 285
Ser Arg Asp Val Tyr Arg Thr Glu Ser Trp Ala Gly Val Leu Leu Trp
290 295 300
Gly Ile Tyr Leu Glu Pro Ile His Gly Val Pro Thr Val Arg Phe Asn
305 310 315 320
Phe Thr Asn Pro Gln Asn Ile Tyr Asp Arg Gly Thr Ala Asn Tyr Ser
325 330 335
Gln Pro Tyr Glu Ser Pro Gly Leu Gln Leu Lys Asp Ser Glu Thr Glu
340 345 350
Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser Tyr Ser His
355 360 365
Arg Leu Ser His Ile Gly Ile Ile Leu Gln Ser Arg Val Asn Val Pro
370 375 380
Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn Thr Ile Gly
385 390 395 400
Pro Asn Arg Ile Thr Gln Ile Pro Met Val Lys Ala Ser Glu Leu Pro
405 410 415
Gln Gly Thr Thr Val Val Arg Gly Pro Gly Phe Thr Gly Gly Asp Ile
420 425 430
Leu Arg Arg Thr Asn Thr Gly Gly Phe Gly Pro Ile Arg Val Thr Val
435 440 445
Asn Gly Pro Leu Thr Gln Arg Tyr Arg Ile Gly Phe Arg Tyr Ala Ser
450 455 460
Thr Val Asp Phe Asp Phe Phe Val Ser Arg Gly Gly Thr Thr Val Asn
465 470 475 480
Asn Phe Arg Phe Leu Arg Thr Met Asn Ser Gly Asp Glu Leu Lys Tyr
485 490 495
Gly Asn Phe Val Arg Arg Ala Phe Thr Thr Pro Phe Thr Phe Thr Gln
500 505 510
Ile Gln Asp Ile Ile Arg Thr Ser Ile Gln Gly Leu Ser Gly Asn Gly
515 520 525
Glu Val Tyr Ile Asp Lys Ile Glu Ile Ile Pro Val Thr Ala Thr Phe
530 535 540
Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Glu Ala Val Asn Ala Leu
545 550 555 560
Phe Thr Asn Thr Asn Pro Arg Arg Leu Lys Thr Asp Val Thr Asp Tyr
565 570 575
His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser Asp Glu Phe
580 585 590
Cys Leu Asp Glu Lys Arg Glu Leu Leu Glu Lys Val Lys Tyr Ala Lys
595 600 605
Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Thr Ser
610 615 620
Ile Asn Lys Gln Pro Asp Phe Ile Ser Thr Asn Glu Gln Ser Asn Phe
625 630 635 640
Thr Ser Ile His Glu Gln Ser Glu His Gly Trp Trp Gly Ser Glu Asn
645 650 655
Ile Thr Ile Gln Glu Gly Asn Asp Val Phe Lys Glu Asn Tyr Val Thr
660 665 670
Leu Pro Gly Thr Phe Asn Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys
675 680 685
Ile Gly Glu Ser Glu Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly
690 695 700
Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn
705 710 715 720
Ala Lys Pro Glu Thr Leu Asp Val Pro Gly Thr Glu Ser Leu Trp Pro
725 730 735
Leu Ser Val Glu Ser Pro Ile Gly Arg Cys Gly Glu Pro Asn Arg Cys
740 745 750
Ala Pro His Phe Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg Asp
755 760 765
Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp
770 775 780
Val Gly Cys Thr Asp Leu His Glu Asn Leu Gly Val Trp Val Val Phe
785 790 795 800
Lys Ile Lys Thr Gln Glu Gly His Ala Arg Leu Gly Asn Leu Glu Phe
805 810 815
Ile Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ser Arg Val Lys Arg
820 825 830
Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Gln Leu Glu Thr
835 840 845
Lys Arg Val Tyr Thr Glu Ala Lys Glu Ala Val Asp Ala Leu Phe Val
850 855 860
Asp Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Gly Met Ile
865 870 875 880
His Ala Ala Asp Lys Leu Val His Arg Ile Arg Glu Ala Tyr Leu Ser
885 890 895
Glu Leu Pro Val Ile Pro Gly Val Asn Ala Glu Ile Phe Glu Glu Leu
900 905 910
Glu Gly His Ile Ile Thr Ala Ile Ser Leu Tyr Asp Ala Arg Asn Val
915 920 925
Val Lys Asn Gly Asp Phe Asn Asn Gly Leu Thr Cys Trp Asn Val Lys
930 935 940
Gly His Val Asp Val Gln Gln Ser His His Arg Ser Asp Leu Val Ile
945 950 955 960
Pro Glu Trp Glu Ala Glu Val Ser Gln Ala Val Arg Val Cys Pro Gly
965 970 975
Cys Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu
980 985 990
Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn Thr Asp Glu Leu Lys
995 1000 1005
Phe Lys Asn Arg Glu Glu Glu Glu Val Tyr Pro Thr Asp Thr Gly
1010 1015 1020
Thr Cys Asn Asp Tyr Thr Ala His Gln Gly Thr Ala Gly Cys Ala
1025 1030 1035
Asp Ala Cys Asn Ser Arg Asn Ala Gly Tyr Glu Asp Ala Tyr Glu
1040 1045 1050
Val Asp Thr Thr Ala Ser Val Asn Tyr Lys Pro Thr Tyr Glu Glu
1055 1060 1065
Glu Thr Tyr Thr Asp Val Arg Arg Asp Asn His Cys Glu Tyr Asp
1070 1075 1080
Arg Gly Tyr Val Asn Tyr Pro Pro Val Pro Ala Gly Tyr Val Thr
1085 1090 1095
Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Thr Val Trp Ile Glu
1100 1105 1110
Ile Gly Glu Thr Glu Gly Lys Phe Ile Val Asp Ser Val Glu Leu
1115 1120 1125
Leu Leu Met Glu Glu
1130
<210> 72
<211> 640
<212> PRT
<213> 苏云金芽孢杆菌
<400> 72
Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser
1 5 10 15
Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asp Leu Ser Pro Asp
20 25 30
Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp
35 40 45
Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly
50 55 60
Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Leu Ala Ser
65 70 75 80
Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Ser Gly Arg Asp Pro
85 90 95
Trp Glu Ile Phe Met Glu His Val Glu Gln Leu Val Arg Gln Gln Ile
100 105 110
Thr Asp Ser Val Arg Asp Thr Ala Ile Ala Arg Leu Glu Gly Leu Gly
115 120 125
Arg Gly Tyr Arg Ser Tyr Gln Gln Ala Leu Glu Thr Trp Leu Asp Asn
130 135 140
Arg Asn Asp Ala Arg Ser Arg Ser Ile Ile Leu Glu Arg Tyr Ile Ala
145 150 155 160
Leu Glu Leu Asp Ile Thr Thr Ala Ile Pro Leu Phe Ser Ile Arg Asn
165 170 175
Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His
180 185 190
Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Trp Gly Met
195 200 205
Ser Ser Ala Asp Val Asn Gln Tyr Tyr Gln Glu Gln Ile Arg Tyr Thr
210 215 220
Glu Glu Tyr Ser Asn His Cys Val Gln Trp Tyr Asn Thr Gly Leu Asn
225 230 235 240
Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Val Arg Tyr Asn Gln Phe
245 250 255
Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro
260 265 270
Ser Tyr Asp Thr Arg Thr Tyr Pro Ile Asn Thr Ser Ala Gln Leu Thr
275 280 285
Arg Glu Val Tyr Thr Asp Ala Ile Gly Ala Thr Gly Val Asn Met Ala
290 295 300
Asn Met Asn Trp Tyr Asn Asn Asn Ala Pro Ser Phe Ser Ala Ile Glu
305 310 315 320
Ala Ala Val Ile Arg Ser Pro His Leu Leu Asp Phe Leu Glu Gln Leu
325 330 335
Thr Ile Phe Ser Ala Ser Ser Arg Trp Ser Asn Thr Arg His Met Thr
340 345 350
Tyr Trp Arg Gly His Thr Ile Gln Ser Arg Pro Ile Gly Gly Gly Leu
355 360 365
Asn Thr Ser Thr Tyr Gly Ser Thr Asn Thr Ser Ile Asn Pro Val Thr
370 375 380
Leu Arg Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Trp Ala Gly
385 390 395 400
Val Leu Leu Trp Gly Ile Tyr Leu Glu Pro Ile His Gly Val Pro Thr
405 410 415
Val Arg Phe Asn Phe Thr Asn Pro Gln Asn Ile Tyr Asp Arg Gly Thr
420 425 430
Ala Asn Tyr Ser Gln Pro Tyr Glu Ser Pro Gly Leu Gln Leu Lys Asp
435 440 445
Ser Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu
450 455 460
Ser Tyr Ser His Arg Leu Ser His Ile Gly Ile Ile Leu Gln Ser Arg
465 470 475 480
Val Asn Val Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr
485 490 495
Asn Thr Ile Gly Pro Asn Arg Ile Thr Gln Ile Pro Met Val Lys Ala
500 505 510
Ser Glu Leu Pro Gln Gly Thr Thr Val Val Arg Gly Pro Gly Phe Thr
515 520 525
Gly Gly Asp Ile Leu Arg Arg Thr Asn Thr Gly Gly Phe Gly Pro Ile
530 535 540
Arg Val Thr Val Asn Gly Pro Leu Thr Gln Arg Tyr Arg Ile Gly Phe
545 550 555 560
Arg Tyr Ala Ser Thr Val Asp Phe Asp Phe Phe Val Ser Arg Gly Gly
565 570 575
Thr Thr Val Asn Asn Phe Arg Phe Leu Arg Thr Met Asn Ser Gly Asp
580 585 590
Glu Leu Lys Tyr Gly Asn Phe Val Arg Arg Ala Phe Thr Thr Pro Phe
595 600 605
Thr Phe Thr Gln Ile Gln Asp Ile Ile Arg Thr Ser Ile Gln Gly Leu
610 615 620
Ser Gly Asn Gly Glu Val Tyr Ile Asp Lys Ile Glu Ile Ile Pro Val
625 630 635 640
<210> 73
<211> 614
<212> PRT
<213> 苏云金芽孢杆菌
<400> 73
Met Asp Leu Ser Pro Asp Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala
1 5 10 15
Glu Gly Asn Asn Ile Asp Pro Phe Val Ser Ala Ser Thr Val Gln Thr
20 25 30
Gly Ile Asn Ile Ala Gly Arg Ile Leu Gly Val Leu Gly Val Pro Phe
35 40 45
Ala Gly Gln Leu Ala Ser Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp
50 55 60
Pro Ser Gly Arg Asp Pro Trp Glu Ile Phe Met Glu His Val Glu Gln
65 70 75 80
Leu Val Arg Gln Gln Ile Thr Asp Ser Val Arg Asp Thr Ala Ile Ala
85 90 95
Arg Leu Glu Gly Leu Gly Arg Gly Tyr Arg Ser Tyr Gln Gln Ala Leu
100 105 110
Glu Thr Trp Leu Asp Asn Arg Asn Asp Ala Arg Ser Arg Ser Ile Ile
115 120 125
Leu Glu Arg Tyr Ile Ala Leu Glu Leu Asp Ile Thr Thr Ala Ile Pro
130 135 140
Leu Phe Ser Ile Arg Asn Gln Glu Val Pro Leu Leu Met Val Tyr Ala
145 150 155 160
Gln Ala Ala Asn Leu His Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe
165 170 175
Gly Ser Glu Trp Gly Met Ser Ser Ala Asp Val Asn Gln Tyr Tyr Gln
180 185 190
Glu Gln Ile Arg Tyr Thr Glu Glu Tyr Ser Asn His Cys Val Gln Trp
195 200 205
Tyr Asn Thr Gly Leu Asn Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp
210 215 220
Val Arg Tyr Asn Gln Phe Arg Arg Asp Leu Thr Leu Gly Val Leu Asp
225 230 235 240
Leu Val Ala Leu Phe Pro Ser Tyr Asp Thr Arg Thr Tyr Pro Ile Asn
245 250 255
Thr Ser Ala Gln Leu Thr Arg Glu Val Tyr Thr Asp Ala Ile Gly Ala
260 265 270
Thr Gly Val Asn Met Ala Asn Met Asn Trp Tyr Asn Asn Asn Ala Pro
275 280 285
Ser Phe Ser Ala Ile Glu Ala Ala Val Ile Arg Ser Pro His Leu Leu
290 295 300
Asp Phe Leu Glu Gln Leu Thr Ile Phe Ser Ala Ser Ser Arg Trp Ser
305 310 315 320
Asn Thr Arg His Met Thr Tyr Trp Arg Gly His Thr Ile Gln Ser Arg
325 330 335
Pro Ile Gly Gly Gly Leu Asn Thr Ser Thr Tyr Gly Ser Thr Asn Thr
340 345 350
Ser Ile Asn Pro Val Thr Leu Arg Phe Thr Ser Arg Asp Val Tyr Arg
355 360 365
Thr Glu Ser Trp Ala Gly Val Leu Leu Trp Gly Ile Tyr Leu Glu Pro
370 375 380
Ile His Gly Val Pro Thr Val Arg Phe Asn Phe Thr Asn Pro Gln Asn
385 390 395 400
Ile Tyr Asp Arg Gly Thr Ala Asn Tyr Ser Gln Pro Tyr Glu Ser Pro
405 410 415
Gly Leu Gln Leu Lys Asp Ser Glu Thr Glu Leu Pro Pro Glu Thr Thr
420 425 430
Glu Arg Pro Asn Tyr Glu Ser Tyr Ser His Arg Leu Ser His Ile Gly
435 440 445
Ile Ile Leu Gln Ser Arg Val Asn Val Pro Val Tyr Ser Trp Thr His
450 455 460
Arg Ser Ala Asp Arg Thr Asn Thr Ile Gly Pro Asn Arg Ile Thr Gln
465 470 475 480
Ile Pro Met Val Lys Ala Ser Glu Leu Pro Gln Gly Thr Thr Val Val
485 490 495
Arg Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr Asn Thr
500 505 510
Gly Gly Phe Gly Pro Ile Arg Val Thr Val Asn Gly Pro Leu Thr Gln
515 520 525
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1

Claims (24)

1.重组核酸分子，该重组核酸分子包括核苷酸序列，该核苷酸序列编码具有杀有害生物活性的氨基酸序列，其中所述核苷酸序列选自下组，该组由以下各项组成：
a)在SEQ ID NO：1-20的任一项中列出的核苷酸序列；
b)编码如下多肽的核苷酸序列，该多肽包括SEQ ID NO：21-74中任一项的氨基酸序列；
c)编码如下多肽的核苷酸序列，该多肽包括与SEQ ID NO：21-74中任一项的氨基酸序列具有至少95％序列一致性的氨基酸序列。
2.如权利要求1所述的重组核酸分子，其中所述核苷酸序列是已经被设计为用于在植物中表达的合成序列。
3.如权利要求1所述的重组核酸分子，其中所述核苷酸序列被可操作地连接至能够指导所述核苷酸序列在植物细胞中表达的启动子上。
4.表达盒，该表达盒包括如权利要求1所述的重组核酸分子。
5.如权利要求4所述的表达盒，该表达盒进一步包括编码异源多肽的核酸分子。
6.包括如权利要求1所述的重组核酸的宿主细胞。
7.如权利要求6所述的宿主细胞，该宿主细胞是细菌宿主细胞。
8.如权利要求6所述的宿主细胞，该宿主细胞是植物细胞。
9.包括如权利要求8所述的宿主细胞的转基因植物。
10.如权利要求9所述的转基因植物，其中所述植物选自下组，该组由以下各项组成：玉米、高粱、小麦、甘蓝、向日葵、番茄、十字花科植物、胡椒、马铃薯、棉花、水稻、大豆、甜菜、甘蔗、烟草、大麦、以及油菜。
11.包括如权利要求1所述的核酸分子的转基因种子。
12.具有杀有害生物活性的重组多肽，该多肽选自下组，该组由以下各项组成：
a)包括SEQ ID NO：21-74中任一项的氨基酸序列的多肽；以及
b)包括与SEQ ID NO：21-74中任一项的氨基酸序列具有至少95％序列一致性的氨基酸序列的多肽。
13.如权利要求12所述的多肽，该多肽进一步包括异源氨基酸序列。
14.包括如权利要求12所述的多肽的组合物。
15.如权利要求14所述的组合物，其中所述组合物选自下组，该组由以下各项组成：粉末、粉剂、小丸、颗粒、喷雾剂、乳液、胶体、以及溶液。
16.如权利要求14所述的组合物，其中所述组合物是通过将细菌细胞的培养物进行干燥、冷冻干燥、均质化、提取、过滤、离心、沉降、或浓缩而制备的。
17.如权利要求14所述的组合物，该组合物包括按重量计从约1％至约99％的所述多肽。
18.用于控制鳞翅目、半翅目、鞘翅目、线虫、或双翅目有害生物种群的方法，该方法包括使所述种群与杀有害生物有效量的如权利要求12所述的多肽接触。
19.用于杀死鳞翅目、半翅目、鞘翅目、线虫、或双翅目有害生物的方法，该方法包括使所述有害生物接触或向所述有害生物喂食杀有害生物有效量的如权利要求12所述的多肽。
20.用于生产具有杀有害生物活性的多肽的方法，该方法包括在表达编码该多肽的核酸分子的条件下培养如权利要求6所述的宿主细胞。
21.植物或植物细胞，该植物或植物细胞将DNA构建体稳定地结合到它的基因组中，该DNA构建体包括对具有杀有害生物活性的蛋白进行编码的核苷酸序列，其中所述核苷酸序列选自下组，该组由以下各项组成：
a)在SEQ ID NO：1-20的任一项中列出的核苷酸序列；
b)编码如下多肽的核苷酸序列，该多肽包括SEQ ID NO：21-74中任一项的氨基酸序列；以及
c)编码如下多肽的核苷酸序列，该多肽包括与SEQ ID NO：21-74中任一项的氨基酸序列具有至少95％序列一致性的氨基酸序列。
22.用于保护植物免受有害生物侵害的方法，该方法包括在植物或其细胞中表达对杀有害生物多肽进行编码的核苷酸序列，其中所述核苷酸序列选自下组，该组由以下各项组成：
a)在SEQ ID NO：1-20的任一项中列出的核苷酸序列；
b)编码如下多肽的核苷酸序列，该多肽包括SEQ ID NO：21-74中任一项的氨基酸序列；以及
c)编码如下多肽的核苷酸序列，该多肽包括与SEQ ID NO：21-74中任一项的氨基酸序列具有至少95％序列一致性的氨基酸序列。
23.如权利要求22所述的方法，其中所述植物产生杀有害生物多肽，该杀有害生物多肽具有针对鳞翅目、半翅目、鞘翅目、线虫、或双翅目有害生物的杀有害生物活性。
24.用于增加植物的产量的方法，该方法包括在田地中使将DNA构建体稳定结合到它的基因组中的植物或其种子生长，该DNA构建体包括对具有杀有害生物活性的蛋白进行编码的核苷酸序列，其中所述核苷酸序列选自下组，该组由以下各项组成：
a)在SEQ ID NO：1-20的任一项中列出的核苷酸序列；
b)编码如下多肽的核苷酸序列，该多肽包括SEQ ID NO：21-74中任一项的氨基酸序列；以及
c)编码如下多肽的核苷酸序列，该多肽包括与SEQ ID NO：21-74中任一项的氨基酸序列具有至少95％序列一致性的氨基酸序列；
其中所述田地被有害生物侵染，所述多肽具有针对该有害生物的杀有害生物活性。
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