DE60142628D1 - Verfahren zur einbringung von molekülen in zellen - Google Patents

Verfahren zur einbringung von molekülen in zellen

Info

Publication number
DE60142628D1
DE60142628D1 DE60142628T DE60142628T DE60142628D1 DE 60142628 D1 DE60142628 D1 DE 60142628D1 DE 60142628 T DE60142628 T DE 60142628T DE 60142628 T DE60142628 T DE 60142628T DE 60142628 D1 DE60142628 D1 DE 60142628D1
Authority
DE
Germany
Prior art keywords
cells
directed
present
introducing molecules
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60142628T
Other languages
English (en)
Inventor
Bruce Marvin Held
Herbert Martin Wilson
Liming Hou
Carol Jean Lewnau
Janelle Christine Eby
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Midwest Oilseeds Inc
Original Assignee
Midwest Oilseeds Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/724,265 external-priority patent/US6809232B1/en
Application filed by Midwest Oilseeds Inc filed Critical Midwest Oilseeds Inc
Application granted granted Critical
Publication of DE60142628D1 publication Critical patent/DE60142628D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C12N15/895—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
DE60142628T 2000-11-28 2001-11-28 Verfahren zur einbringung von molekülen in zellen Expired - Lifetime DE60142628D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/724,265 US6809232B1 (en) 1999-11-29 2000-11-28 Methods and compositions for the introduction of molecules into cells
PCT/US2001/044190 WO2002044391A2 (en) 2000-11-28 2001-11-28 Methods and compositions for the introduction of molecules into cells

Publications (1)

Publication Number Publication Date
DE60142628D1 true DE60142628D1 (de) 2010-09-02

Family

ID=24909723

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60142628T Expired - Lifetime DE60142628D1 (de) 2000-11-28 2001-11-28 Verfahren zur einbringung von molekülen in zellen

Country Status (6)

Country Link
EP (1) EP1337140B1 (de)
AT (1) ATE474454T1 (de)
AU (2) AU2002239343B2 (de)
CA (1) CA2429272C (de)
DE (1) DE60142628D1 (de)
WO (1) WO2002044391A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7314969B2 (en) 1999-11-29 2008-01-01 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
EP1250447B1 (de) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Verfahren, Medien und Vorrichtung zur Einführung von Molekülen in Pflanzenzellen und Bakterien mittels Aerosolstrahlen
DE60333435D1 (de) 2003-02-03 2010-09-02 Bioware Technology Co Ltd Genpistole mit Niederdruckgasbeschleunigung

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3854321A (en) * 1973-04-27 1974-12-17 B Dahneke Aerosol beam device and method
US5240842A (en) * 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
AU642889B2 (en) * 1989-07-11 1993-11-04 Biotechnology Research And Development Corporation Aerosol beam microinjector
ATE130371T1 (de) * 1989-12-19 1995-12-15 Ciba Geigy Ag Verfahren und vorrichtung zur genetischen transformation von zellen.
US6433154B1 (en) * 1997-06-12 2002-08-13 Bristol-Myers Squibb Company Functional receptor/kinase chimera in yeast cells
EP1250447B1 (de) * 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Verfahren, Medien und Vorrichtung zur Einführung von Molekülen in Pflanzenzellen und Bakterien mittels Aerosolstrahlen

Also Published As

Publication number Publication date
WO2002044391A2 (en) 2002-06-06
CA2429272A1 (en) 2002-06-06
ATE474454T1 (de) 2010-08-15
EP1337140B1 (de) 2010-07-21
WO2002044391A3 (en) 2003-01-23
WO2002044391A9 (en) 2003-05-01
EP1337140A2 (de) 2003-08-27
CA2429272C (en) 2016-06-28
AU3934302A (en) 2002-06-11
AU2002239343B2 (en) 2007-10-04

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