ATE533838T1 - Verfahren zur verbesserten einführung von dna in bakterienzellen - Google Patents

Verfahren zur verbesserten einführung von dna in bakterienzellen

Info

Publication number
ATE533838T1
ATE533838T1 AT07868936T AT07868936T ATE533838T1 AT E533838 T1 ATE533838 T1 AT E533838T1 AT 07868936 T AT07868936 T AT 07868936T AT 07868936 T AT07868936 T AT 07868936T AT E533838 T1 ATE533838 T1 AT E533838T1
Authority
AT
Austria
Prior art keywords
dna
bacterial cells
improved introduction
introduction
improved
Prior art date
Application number
AT07868936T
Other languages
English (en)
Inventor
Michael Thomas
Michael Rey
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE533838T1 publication Critical patent/ATE533838T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT07868936T 2006-11-29 2007-11-29 Verfahren zur verbesserten einführung von dna in bakterienzellen ATE533838T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US86189606P 2006-11-29 2006-11-29
PCT/US2007/085840 WO2008067423A2 (en) 2006-11-29 2007-11-29 Methods of improving the introduction of dna into bacterial cells

Publications (1)

Publication Number Publication Date
ATE533838T1 true ATE533838T1 (de) 2011-12-15

Family

ID=39468705

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07868936T ATE533838T1 (de) 2006-11-29 2007-11-29 Verfahren zur verbesserten einführung von dna in bakterienzellen

Country Status (6)

Country Link
US (4) US7820408B2 (de)
EP (1) EP2089516B1 (de)
CN (2) CN101595214B (de)
AT (1) ATE533838T1 (de)
DK (1) DK2089516T3 (de)
WO (1) WO2008067423A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2689015B1 (de) 2011-03-23 2015-01-07 Novozymes A/S Verfahren zur herstellung sezernierter polypeptide
US9045758B2 (en) 2013-03-13 2015-06-02 Coskata, Inc. Use of clostridial methyltransferases for generating novel strains
CN105002162A (zh) * 2015-08-05 2015-10-28 广东省微生物研究所 一种用于转化不同菌株的外源质粒体外甲基化修饰的方法
EP3519573A1 (de) * 2016-09-30 2019-08-07 Invista North America S.a.r.l. Verbesserte verfahren und reagenzien zur manipulation von wirtszellen
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis
WO2018134386A1 (en) 2017-01-23 2018-07-26 Novozymes A/S Host cells and methods for producing double-stranded rna
US11066653B2 (en) * 2017-07-21 2021-07-20 Basf Se Method of transforming bacterial cells
WO2019092042A1 (en) 2017-11-10 2019-05-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
US20220275354A1 (en) 2019-05-15 2022-09-01 Novozymes A/S TEMPERATURE-SENSITIVE RNA- Guided Endonuclease
US20220298517A1 (en) 2019-06-25 2022-09-22 Novozymes A/S Counter-selection by inhibition of conditionally essential genes
EP4370534A1 (de) 2021-07-13 2024-05-22 Novozymes A/S Rekombinante cutinase-expression
CN113652412A (zh) * 2021-09-13 2021-11-16 苏州近岸蛋白质科技股份有限公司 一种制备限制性内切酶类产品的方法
EP4444859A1 (de) 2021-12-10 2024-10-16 Novozymes A/S Verbesserte proteinproduktion in rekombinanten bakterien
EP4630563A1 (de) 2022-12-05 2025-10-15 Novozymes A/S Modifizierte rna-polymeraseaktivitäten
WO2025132815A1 (en) 2023-12-20 2025-06-26 Novozymes A/S Novel cas nucleases and polynucleotides encoding the same

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE113653T1 (de) 1986-06-06 1994-11-15 New England Biolabs Inc Verfahren zur klonierung eines restriktionsmodifizierungssystems.
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DK153992D0 (da) 1992-12-22 1992-12-22 Novo Nordisk As Metode
US5472872A (en) * 1994-01-13 1995-12-05 Mead; David Recombinant CviJI restriction endonuclease
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
EP0817856A1 (de) 1995-03-22 1998-01-14 Novo Nordisk A/S Einfuhrung von dna in bacillus subtilis durch konjugation
US5958728A (en) 1996-11-18 1999-09-28 Novo Nordiskbiotech, Inc. Methods for producing polypeptides in mutants of bacillus cells
US5891701A (en) 1997-06-12 1999-04-06 Novo Nordisk Biotech Inc. Nucleic acids encoding a polypeptide having protease activity
DE69840175D1 (de) * 1997-12-12 2008-12-11 Samyang Corp Biologisch abbaubare gemischte polymerische mizellen zur genverabreichung
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6100063A (en) 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6689573B1 (en) * 1999-05-24 2004-02-10 New England Biolabs, Inc. Method for screening restriction endonucleases
AU1824101A (en) * 1999-06-18 2001-01-09 Elitra Pharmaceuticals, Inc. Nucleotide sequences of moraxella catarrhalis genome
ATE502115T1 (de) 2001-12-21 2011-04-15 Novozymes Biopharma Dk As Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle
DE102004041717A1 (de) * 2004-08-28 2006-03-16 Henkel Kgaa Restriktions-Modifikationssystem von Bacillus licheniformis und darauf aufbauende verbesserte biotechnologische Produktionsverfahren

Also Published As

Publication number Publication date
US20110027857A1 (en) 2011-02-03
US20100055739A1 (en) 2010-03-04
US20130177942A1 (en) 2013-07-11
US8236544B2 (en) 2012-08-07
US8361771B2 (en) 2013-01-29
US20120276638A1 (en) 2012-11-01
DK2089516T3 (da) 2012-02-20
CN101595214A (zh) 2009-12-02
WO2008067423A2 (en) 2008-06-05
EP2089516A2 (de) 2009-08-19
US9580700B2 (en) 2017-02-28
WO2008067423A3 (en) 2008-11-13
US7820408B2 (en) 2010-10-26
CN103255114B (zh) 2015-03-25
EP2089516B1 (de) 2011-11-16
CN101595214B (zh) 2013-06-12
CN103255114A (zh) 2013-08-21

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