ATE502115T1 - Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle - Google Patents

Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle

Info

Publication number
ATE502115T1
ATE502115T1 AT02805661T AT02805661T ATE502115T1 AT E502115 T1 ATE502115 T1 AT E502115T1 AT 02805661 T AT02805661 T AT 02805661T AT 02805661 T AT02805661 T AT 02805661T AT E502115 T1 ATE502115 T1 AT E502115T1
Authority
AT
Austria
Prior art keywords
udp
glucose
gene
host cell
hyaluronan synthase
Prior art date
Application number
AT02805661T
Other languages
English (en)
Inventor
Alan Sloma
Regine Behr
William Widner
Maria Tang
David Sternberg
Stephen Brown
Original Assignee
Novozymes Biopharma Dk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biopharma Dk As filed Critical Novozymes Biopharma Dk As
Application granted granted Critical
Publication of ATE502115T1 publication Critical patent/ATE502115T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT02805661T 2001-12-21 2002-12-20 Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle ATE502115T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34264401P 2001-12-21 2001-12-21
PCT/US2002/041067 WO2003054163A2 (en) 2001-12-21 2002-12-20 Methods for producing hyaluronan in a recombinant host cell

Publications (1)

Publication Number Publication Date
ATE502115T1 true ATE502115T1 (de) 2011-04-15

Family

ID=23342668

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02805661T ATE502115T1 (de) 2001-12-21 2002-12-20 Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle

Country Status (18)

Country Link
US (5) US7811806B2 (de)
EP (1) EP1572895B1 (de)
JP (1) JP2005525091A (de)
KR (2) KR100885163B1 (de)
CN (1) CN1636052B (de)
AT (1) ATE502115T1 (de)
AU (1) AU2002366711C1 (de)
BR (1) BR0215220A (de)
CA (2) CA2803931A1 (de)
CZ (1) CZ2004765A3 (de)
DE (1) DE60239495D1 (de)
DK (1) DK1572895T3 (de)
ES (1) ES2362354T3 (de)
HU (2) HU228693B1 (de)
IL (2) IL162302A0 (de)
PL (2) PL212928B1 (de)
RU (1) RU2346049C2 (de)
WO (1) WO2003054163A2 (de)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7091008B1 (en) * 1994-07-01 2006-08-15 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof in Bacillus hosts
EP1025211B1 (de) * 1997-10-31 2004-12-15 The Board of Regents of The University of Oklahoma Hyaluronan synthase gen und seine verwendung
CN1620511A (zh) * 2001-06-13 2005-05-25 俄克拉何马大学董事会 透明质素合成酶基因及其表达
KR100885163B1 (ko) * 2001-12-21 2009-02-23 노보자임스 바이오폴리머 에이/에스 재조합체 숙주 세포에서 히알루로난의 제조 방법
ES2359381T5 (es) * 2002-05-14 2014-07-10 Novozymes A/S Variantes de pectato liasa
KR101023049B1 (ko) * 2002-06-20 2011-03-24 노보자임스 바이오파마 디케이 에이/에스 2가 염을 사용한 응집 방법
DK1537229T3 (da) * 2002-07-26 2013-12-02 Novozymes Inc Fremgangsmåder til produktion af biologiske stoffer i pigmentmanglende mutanter af Bacillus-celler
EP3018140A3 (de) * 2004-01-09 2016-08-17 Basf Se Inaktivierung von glutamylpolypeptidsynthese in bacillus
EP1735455A2 (de) * 2004-03-31 2006-12-27 Novozymes Biopolymer A/S Verfahren zur produktion von hyaluronsäure in einer bacillus-zelle
US7002007B2 (en) * 2004-05-28 2006-02-21 Calcigen Corporation Production of high molecular weight hyaluronates
US20080044853A1 (en) 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
EP1640457A1 (de) * 2004-09-23 2006-03-29 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan
SI1805312T1 (sl) 2004-09-23 2009-12-31 Bayer Cropscience Ag Postopki in sredstva za izdelavo hialuronana
PL1817347T3 (pl) * 2004-11-24 2017-10-31 Albumedix As Sposób sieciowania kwasu hialuronowego za pomocą diwinylosulfonu
EP1833882A1 (de) * 2004-12-30 2007-09-19 Novozymes Biopolymer A/S Mit einem polymer einer alpha-hydroxysäure verknüpfte hyaluronsäure
EP1696032A1 (de) * 2005-02-23 2006-08-30 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan in Pilzen
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP4609177B2 (ja) 2005-04-28 2011-01-12 チッソ株式会社 カルシウム結合型発光蛋白質溶液の発光時間延長方法
US20060287512A1 (en) * 2005-05-04 2006-12-21 Novozymes Biopolymer A/S Method of controlling the content of selected component(s) from polymer(s) using molecular sieve(s)
BRPI0614885A2 (pt) * 2005-08-25 2011-04-19 Toyo Boseki planta produtora de ácido hialurÈnico
EP1951878B1 (de) * 2005-10-05 2015-02-25 Bayer Intellectual Property GmbH Pflanzen mit verstärkter hyaluronanproduktion
EP1772052A1 (de) 2005-10-05 2007-04-11 Bayer CropScience GmbH Verbesserte Verfahren und Mittel zur Herstellung von Hyaluronan
JP2007174957A (ja) * 2005-12-27 2007-07-12 Toyobo Co Ltd ヒアルロン酸生産酵母
US20080038780A1 (en) 2006-02-15 2008-02-14 Novozymes Biopolymer A/S Production of low molecular weight hyaluronic acid
BRPI0708776A2 (pt) * 2006-03-14 2011-06-14 Novozymes Biopolymer As produto de Ácido hialurânico acrilado, e, processo para preparaÇço e mÉtodo para polimerizaÇço/reticulaÇço do mesmo
AU2007280846B2 (en) 2006-08-04 2012-06-14 Novozymes Biopharma Dk A/S Branched hyaluronic acid and method of manufacture
ATE533838T1 (de) 2006-11-29 2011-12-15 Novozymes Inc Verfahren zur verbesserten einführung von dna in bakterienzellen
CN101611145B (zh) 2006-12-21 2013-08-14 诺维信股份有限公司 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列
EP2031053A1 (de) * 2007-08-31 2009-03-04 The University Of Queensland HA-Herstellung
US20110281817A1 (en) * 2007-08-31 2011-11-17 Sugar Industry Innovation Pty. Ltd. Production of hyaluronic acid
EP2036983A1 (de) * 2007-09-12 2009-03-18 Bayer CropScience AG Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren
JP5523338B2 (ja) 2007-12-19 2014-06-18 エヴォニク ゴールドシュミット ゲーエムベーハー エマルジョン中の架橋ヒアルロン酸
WO2010068955A2 (en) 2008-12-13 2010-06-17 Dna Microarray MICROENVIRONMENT NICHE ASSAY FOR CiPS SCREENING
EP2213315A1 (de) 2009-01-30 2010-08-04 Mero S.r.L. Antibakterielles Hydrogel und dessen Verwendungen in der Orthopädie
CN102021213B (zh) * 2009-09-17 2014-07-09 上海佰加壹医药有限公司 一种发酵生产透明质酸发酵液的方法
CN102884182A (zh) 2010-03-03 2013-01-16 诺维信股份有限公司 木聚糖酶变体及编码其的多核苷酸
IT1401498B1 (it) 2010-07-30 2013-07-26 Mero Srl Idrogelo a base di acido ialuronico e suo uso in ortopedia
IT1402385B1 (it) * 2010-09-09 2013-09-04 Fidia Farmaceutici Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis
IT1402384B1 (it) 2010-09-09 2013-09-04 Fidia Farmaceutici Processo per la produzione di acido ialuronico in escherichia coli o bacillus megaterium
CA2835016A1 (en) 2011-05-30 2012-12-06 Novozymes Biopharma Dk A/S Spray drying of high molecular weight hyaluronic acid
CN102911887A (zh) * 2011-08-02 2013-02-06 山东省生物药物研究院 一种利用毕赤酵母基因调控发酵生产不同分子量透明质酸的方法
CA2847328A1 (en) 2011-09-02 2013-03-07 Novozymes Biopharma Dk A/S Oral formulations containing hyaluronic acid for sustained drug release
DE102012201297A1 (de) * 2012-01-31 2013-08-01 Basf Se Expressionsverfahren
CN102559559A (zh) * 2012-02-21 2012-07-11 山东福瑞达生物医药有限公司 一种芽孢杆菌及采用该菌种生产透明质酸酶的方法
US9493755B2 (en) * 2012-02-21 2016-11-15 Bloomage Freda Biopharm Co., Ltd. Bacillus, hyaluronidase, and uses thereof
JP6306519B2 (ja) * 2012-02-22 2018-04-04 ノボザイムス アクティーゼルスカブ 高度発酵制御
WO2014100837A1 (en) 2012-12-17 2014-06-26 Bui The Duy Computer aided implantation of body implants
CN105873955B (zh) 2013-11-25 2019-03-15 杜特里亚生物材料有限责任公司 富氘透明质酸
CA2896038C (en) 2015-07-03 2022-08-09 Glycobiosciences Inc. Polymer matrix compositions comprising a high concentration of bio-fermented sodium hyaluronate and uses thereof
CN106755022B (zh) * 2015-11-25 2020-08-04 中国科学院大连化学物理研究所 乙酰葡萄糖胺磷酸变位酶AtAGM编码基因及其酶、制备、应用与酶活性检测方法
WO2018007435A1 (en) 2016-07-05 2018-01-11 Novozymes A/S Pectate lyase variants and polynucleotides encoding same
CN107653280B (zh) * 2016-07-25 2021-05-04 安琪酵母股份有限公司 透明质酸发酵用的组合物、培养基和方法及其应用
CN106381279B (zh) * 2016-08-29 2019-11-05 中国药科大学 一种细菌胞外多糖、其制备方法及其应用
WO2020122429A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
KR102135044B1 (ko) * 2018-12-10 2020-07-17 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
WO2020122430A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 비병원성 세균을 이용하여 히알루론산 생산을 위한 발현 시스템 및 상기 발현 시스템을 이용한 히알루론산 생산방법
CN110055201B (zh) * 2019-03-05 2021-05-28 江南大学 一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法
RU2723722C1 (ru) * 2019-07-26 2020-06-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ
RU2719140C1 (ru) 2019-07-26 2020-04-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии
CN111518825B (zh) * 2020-04-30 2022-10-11 浙江工业大学 一种多基因组合表达制备蛹虫草多糖的方法
EP4294823A1 (de) * 2021-02-22 2023-12-27 Danisco US Inc. Verfahren und zusammensetzungen zur herstellung von proteinen von interesse in pigmentdefizienten bacillus-zellen

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60251898A (ja) * 1984-05-25 1985-12-12 Shiseido Co Ltd 醗酵法によるヒアルロン酸の製造方法
US6455304B1 (en) 1994-07-01 2002-09-24 The Board Of Regents Of The University Of Oklahoma Hyaluronate synthase gene and uses thereof
US6951743B2 (en) 1997-10-31 2005-10-04 University Of Oklahoma Board Of Regents Hyaluronan synthase genes and expression thereof in bacillus hosts
IT1271001B (it) 1994-07-26 1997-05-26 Poli Ind Chimica Spa Procedimento di preparazione di acido ialuronico mediante fermentazione con streptococcus
EP1025211B1 (de) 1997-10-31 2004-12-15 The Board of Regents of The University of Oklahoma Hyaluronan synthase gen und seine verwendung
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
BR9909346A (pt) * 1998-04-02 2001-09-11 Univ Oklahoma Segmento de ácido nucleico, vetor recombinante, célula hospedeira, composição, processos para detectar uma espécie de dna, uma célula bacteriana e infecção, para produzir ácido hialurÈnico e para criar uma vacina, sequência de ácido nucleico e de cdna, vacina e teste diagnóstico
EP1129209B1 (de) 1998-11-11 2009-01-21 The Board Of Regents Of The University Of Oklahoma Polymerpfropfung mittels polysaccharide-synthase
WO2002077183A2 (en) 2001-03-21 2002-10-03 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
CN1620511A (zh) * 2001-06-13 2005-05-25 俄克拉何马大学董事会 透明质素合成酶基因及其表达
KR100885163B1 (ko) * 2001-12-21 2009-02-23 노보자임스 바이오폴리머 에이/에스 재조합체 숙주 세포에서 히알루로난의 제조 방법

Also Published As

Publication number Publication date
WO2003054163A2 (en) 2003-07-03
PL375190A1 (en) 2005-11-28
CA2803931A1 (en) 2003-07-03
US20110189737A1 (en) 2011-08-04
PL399352A1 (pl) 2012-10-08
CN1636052B (zh) 2014-07-02
HUP0700154A2 (en) 2007-05-29
DE60239495D1 (de) 2011-04-28
CA2471148A1 (en) 2003-07-03
ES2362354T3 (es) 2011-07-04
US20120149067A1 (en) 2012-06-14
AU2002366711A1 (en) 2003-07-09
US20110014662A1 (en) 2011-01-20
EP1572895A4 (de) 2007-07-25
KR20080018965A (ko) 2008-02-28
KR100885163B1 (ko) 2009-02-23
US20030175902A1 (en) 2003-09-18
CZ2004765A3 (cs) 2004-12-15
US8137951B2 (en) 2012-03-20
CA2471148C (en) 2013-04-09
AU2002366711B2 (en) 2007-11-29
JP2005525091A (ja) 2005-08-25
HU1300136D0 (hu) 2007-05-29
CN1636052A (zh) 2005-07-06
RU2346049C2 (ru) 2009-02-10
BR0215220A (pt) 2007-01-09
KR100879908B1 (ko) 2009-01-21
AU2002366711C1 (en) 2009-01-22
KR20040085142A (ko) 2004-10-07
PL212928B1 (pl) 2012-12-31
US8574886B2 (en) 2013-11-05
US7811806B2 (en) 2010-10-12
RU2004122420A (ru) 2005-10-10
US8093036B2 (en) 2012-01-10
EP1572895A2 (de) 2005-09-14
HU228693B1 (hu) 2013-05-28
EP1572895B1 (de) 2011-03-16
DK1572895T3 (da) 2011-07-11
US20140038235A1 (en) 2014-02-06
IL162302A (en) 2011-01-31
WO2003054163A3 (en) 2005-10-27
IL162302A0 (en) 2005-11-20

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