IT1402385B1 - Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis - Google Patents

Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis

Info

Publication number
IT1402385B1
IT1402385B1 ITMI2010A001642A ITMI20101642A IT1402385B1 IT 1402385 B1 IT1402385 B1 IT 1402385B1 IT MI2010A001642 A ITMI2010A001642 A IT MI2010A001642A IT MI20101642 A ITMI20101642 A IT MI20101642A IT 1402385 B1 IT1402385 B1 IT 1402385B1
Authority
IT
Italy
Prior art keywords
production
escherichia coli
hyaluronic acid
bacillus subtilis
subtilis
Prior art date
Application number
ITMI2010A001642A
Other languages
English (en)
Inventor
Susanna Vaccaro
Vincenza Corsa
Alessandro Negro
Original Assignee
Fidia Farmaceutici
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fidia Farmaceutici filed Critical Fidia Farmaceutici
Priority to ITMI2010A001642A priority Critical patent/IT1402385B1/it
Priority to ES11764126.6T priority patent/ES2593613T3/es
Priority to PCT/EP2011/065642 priority patent/WO2012032154A1/en
Priority to EP11764126.6A priority patent/EP2614088B1/en
Priority to US13/821,947 priority patent/US9290785B2/en
Publication of ITMI20101642A1 publication Critical patent/ITMI20101642A1/it
Application granted granted Critical
Publication of IT1402385B1 publication Critical patent/IT1402385B1/it
Priority to US15/045,704 priority patent/US10017802B2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01022—UDP-glucose 6-dehydrogenase (1.1.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01212—Hyaluronan synthase (2.4.1.212)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Materials Engineering (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
ITMI2010A001642A 2010-09-09 2010-09-09 Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis IT1402385B1 (it)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ITMI2010A001642A IT1402385B1 (it) 2010-09-09 2010-09-09 Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis
ES11764126.6T ES2593613T3 (es) 2010-09-09 2011-09-09 Proceso para la producción de ácido hialurónico en Escherichia coli o Bacillus subtilis
PCT/EP2011/065642 WO2012032154A1 (en) 2010-09-09 2011-09-09 Process for the production of hyaluronic acid in escherichia coli or bacillus subtilis
EP11764126.6A EP2614088B1 (en) 2010-09-09 2011-09-09 Process for the production of hyaluronic acid in escherichia coli or bacillus subtilis
US13/821,947 US9290785B2 (en) 2010-09-09 2011-09-09 Method for obtaining recombinant host bacterial cells that produce hyaluronic acid
US15/045,704 US10017802B2 (en) 2010-09-09 2016-02-17 Process for the production of hyaluronic acid in Escherichia coli or Bacillus subtilis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI2010A001642A IT1402385B1 (it) 2010-09-09 2010-09-09 Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis

Publications (2)

Publication Number Publication Date
ITMI20101642A1 ITMI20101642A1 (it) 2012-03-10
IT1402385B1 true IT1402385B1 (it) 2013-09-04

Family

ID=43738875

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI2010A001642A IT1402385B1 (it) 2010-09-09 2010-09-09 Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis

Country Status (5)

Country Link
US (2) US9290785B2 (it)
EP (1) EP2614088B1 (it)
ES (1) ES2593613T3 (it)
IT (1) IT1402385B1 (it)
WO (1) WO2012032154A1 (it)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITPD20120173A1 (it) 2012-05-31 2013-12-01 Fidia Farmaceutici "nuovo sistema di rilascio di proteine idrofobiche"
ITPD20120360A1 (it) 2012-11-30 2014-05-31 Fidia Farmaceutici "nuovi geli viscoelastici in chirurgia oftalmica"
ITPD20130110A1 (it) 2013-04-24 2014-10-25 Fidia Farmaceutici Composizioni farmaceutiche comprendenti acido ialuronico per il trattamento del black disc
ITMI20130883A1 (it) 2013-05-30 2014-12-01 Eurores S R L Composizioni farmaceutiche comprendenti collagene e sodio ialuronato
ITMI20131971A1 (it) 2013-11-26 2015-05-27 Fidia Farmaceutici Composizioni farmaceutiche ad attività idratante e lubrificante
CN104212732A (zh) * 2014-09-12 2014-12-17 江南大学 一种产透明质酸的重组毕赤酵母及其构建方法
CN105400784B (zh) * 2015-10-10 2019-01-15 清华大学 一种枯草芽孢杆菌诱导性强启动子及其应用
IT201900006250A1 (it) 2019-04-23 2020-10-23 Fidia Farm Spa Medicazione per il trattamento della cute lesa
IT201800009444A1 (it) 2018-10-15 2020-04-15 Fidia Farm Spa Coniugati tra acido ialuronico e amminobisfosfonati e loro impiego terapeutico
IT201800009731A1 (it) 2018-10-25 2020-04-25 Fidia Farm Spa Coniugato paclitaxel - acido ialuronico nel trattamento del carcinoma vescicale non infiltrante
CN109628363B (zh) * 2019-01-03 2022-12-02 南开大学 一株生产高分子量透明质酸的工程菌及其构建方法和应用
CN110055201B (zh) * 2019-03-05 2021-05-28 江南大学 一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法
IT201900003887A1 (it) 2019-03-18 2020-09-18 Fidia Farm Spa Sistemi di stabilizzazione e di rilascio controllato di farmaci instabili alla sterilizzazione
RU2719140C1 (ru) * 2019-07-26 2020-04-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии
RU2723722C1 (ru) * 2019-07-26 2020-06-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ
IT201900019762A1 (it) 2019-10-24 2021-04-24 Fidia Farm Spa Composizione farmaceutica per uso nel trattamento della cistite di varia eziologia
CN110923173B (zh) 2019-12-26 2021-10-08 江南大学 一种肠杆菌及其应用
IT202000005692A1 (it) 2020-03-17 2021-09-17 Fidia Farm Spa Bio-inchiostro per stampa 3D, relativo coniugato e processo di preparazione di un intermedio costituito da un linker fotoreattivo
IT202000007747A1 (it) 2020-04-10 2021-10-10 Fidia Farm Spa Coniugato acido ialuronico-paclitaxel nel trattamento del mesotelioma
IT202000008209A1 (it) 2020-04-17 2021-10-17 Fidia Farm Spa Procedimento di sintesi di un coniugato acido ialuronico-paclitaxel
CN111548978B (zh) * 2020-05-14 2021-11-12 浙江农林大学 一种产甘露聚糖的枯草芽孢杆菌及其应用
IT202000014017A1 (it) 2020-06-11 2021-12-11 Fidia Farm Spa Nuovi derivati ottenuti da acido ialuronico e carnosina
IT202000032243A1 (it) 2020-12-23 2022-06-23 Fidia Farm Spa Nuovi agenti antivirali
CN113564154A (zh) * 2021-08-16 2021-10-29 厦门大学 一种利用氧化石墨烯-羊毛角蛋白与金属离子配位固定化氧化还原酶的方法
IT202100032111A1 (it) 2021-12-22 2023-06-22 Fidia Farm Spa Nuovi sostituti biocompatibili dell’umor vitreo

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9715660D0 (en) * 1997-07-25 1997-10-01 Zeneca Ltd Proteins
PL212928B1 (pl) * 2001-12-21 2012-12-31 Novozymes Biopolymer As Sposób wytwarzania kwasu hialuronowego i komórka gospodarz Bacillus

Also Published As

Publication number Publication date
EP2614088B1 (en) 2016-08-31
ITMI20101642A1 (it) 2012-03-10
WO2012032154A1 (en) 2012-03-15
EP2614088A1 (en) 2013-07-17
US9290785B2 (en) 2016-03-22
US10017802B2 (en) 2018-07-10
US20130189740A1 (en) 2013-07-25
US20160237465A1 (en) 2016-08-18
ES2593613T3 (es) 2016-12-12

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