ES2593613T3 - Proceso para la producción de ácido hialurónico en Escherichia coli o Bacillus subtilis - Google Patents
Proceso para la producción de ácido hialurónico en Escherichia coli o Bacillus subtilis Download PDFInfo
- Publication number
- ES2593613T3 ES2593613T3 ES11764126.6T ES11764126T ES2593613T3 ES 2593613 T3 ES2593613 T3 ES 2593613T3 ES 11764126 T ES11764126 T ES 11764126T ES 2593613 T3 ES2593613 T3 ES 2593613T3
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- sequence encoding
- hyaluronic acid
- enzyme
- bacillus subtilis
- production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01022—UDP-glucose 6-dehydrogenase (1.1.1.22)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01212—Hyaluronan synthase (2.4.1.212)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
- C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Proceso para la producción de ácido hialurónico en Bacillus subtilis, que comprende los siguientes etapas: (a) cultivar células bacterianas huésped de Bacillus subtilis transformadas con el sistema grac-lac, en condiciones adecuadas para la producción de ácido hialurónico, y en presencia de isopropil-ß-tiogalactopiranósido (IPTG) como inductor, en donde dichas células bacterianas huésped se caracterizan por estar transformadas con: (i) al menos un vector de plásmido episómico que comprende una secuencia que codifica para la enzima hialuronano sintasa, una secuencia que codifica para la enzima UDP-glucosa deshidrogenasa en tándem, bajo el control del promotor fuerte inducible Pgrac que usa el represor lac, o (ii) al menos un vector de plásmido episómico que comprende una secuencia que codifica para la enzima hialuronano sintasa, una secuencia que codifica para la enzima UDP-glucosa deshidrogenasa, una secuencia que codifica para la enzima UDP-glucosa pirofosforilasa, y una secuencia que codifica para la enzima glucosa 6 fosfato isomerasa, bajo el control del promotor fuerte inducible Pgrac que usa el represor lac; (b) recuperar el ácido hialurónico del medio de cultivo; en donde dichas células huésped bacterianas de Bacillus subtilis transformadas con el vector de plásmido (i) o (ii) capaces de producir ácido hialurónico de la etapa a) se preseleccionan en la placa en gradiente de IPTG.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI20101642 | 2010-09-09 | ||
ITMI2010A001642A IT1402385B1 (it) | 2010-09-09 | 2010-09-09 | Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis |
PCT/EP2011/065642 WO2012032154A1 (en) | 2010-09-09 | 2011-09-09 | Process for the production of hyaluronic acid in escherichia coli or bacillus subtilis |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2593613T3 true ES2593613T3 (es) | 2016-12-12 |
Family
ID=43738875
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES11764126.6T Active ES2593613T3 (es) | 2010-09-09 | 2011-09-09 | Proceso para la producción de ácido hialurónico en Escherichia coli o Bacillus subtilis |
Country Status (5)
Country | Link |
---|---|
US (2) | US9290785B2 (es) |
EP (1) | EP2614088B1 (es) |
ES (1) | ES2593613T3 (es) |
IT (1) | IT1402385B1 (es) |
WO (1) | WO2012032154A1 (es) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITPD20120173A1 (it) | 2012-05-31 | 2013-12-01 | Fidia Farmaceutici | "nuovo sistema di rilascio di proteine idrofobiche" |
ITPD20120360A1 (it) | 2012-11-30 | 2014-05-31 | Fidia Farmaceutici | "nuovi geli viscoelastici in chirurgia oftalmica" |
ITPD20130110A1 (it) | 2013-04-24 | 2014-10-25 | Fidia Farmaceutici | Composizioni farmaceutiche comprendenti acido ialuronico per il trattamento del black disc |
ITMI20130883A1 (it) | 2013-05-30 | 2014-12-01 | Eurores S R L | Composizioni farmaceutiche comprendenti collagene e sodio ialuronato |
ITMI20131971A1 (it) | 2013-11-26 | 2015-05-27 | Fidia Farmaceutici | Composizioni farmaceutiche ad attività idratante e lubrificante |
CN104212732A (zh) * | 2014-09-12 | 2014-12-17 | 江南大学 | 一种产透明质酸的重组毕赤酵母及其构建方法 |
CN105400784B (zh) * | 2015-10-10 | 2019-01-15 | 清华大学 | 一种枯草芽孢杆菌诱导性强启动子及其应用 |
IT201900006250A1 (it) | 2019-04-23 | 2020-10-23 | Fidia Farm Spa | Medicazione per il trattamento della cute lesa |
IT201800009444A1 (it) | 2018-10-15 | 2020-04-15 | Fidia Farm Spa | Coniugati tra acido ialuronico e amminobisfosfonati e loro impiego terapeutico |
IT201800009731A1 (it) | 2018-10-25 | 2020-04-25 | Fidia Farm Spa | Coniugato paclitaxel - acido ialuronico nel trattamento del carcinoma vescicale non infiltrante |
CN109628363B (zh) * | 2019-01-03 | 2022-12-02 | 南开大学 | 一株生产高分子量透明质酸的工程菌及其构建方法和应用 |
CN110055201B (zh) * | 2019-03-05 | 2021-05-28 | 江南大学 | 一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法 |
IT201900003887A1 (it) | 2019-03-18 | 2020-09-18 | Fidia Farm Spa | Sistemi di stabilizzazione e di rilascio controllato di farmaci instabili alla sterilizzazione |
RU2719140C1 (ru) * | 2019-07-26 | 2020-04-17 | Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" | Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии |
RU2723722C1 (ru) * | 2019-07-26 | 2020-06-17 | Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" | ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ |
IT201900019762A1 (it) | 2019-10-24 | 2021-04-24 | Fidia Farm Spa | Composizione farmaceutica per uso nel trattamento della cistite di varia eziologia |
CN110923173B (zh) | 2019-12-26 | 2021-10-08 | 江南大学 | 一种肠杆菌及其应用 |
IT202000005692A1 (it) | 2020-03-17 | 2021-09-17 | Fidia Farm Spa | Bio-inchiostro per stampa 3D, relativo coniugato e processo di preparazione di un intermedio costituito da un linker fotoreattivo |
IT202000007747A1 (it) | 2020-04-10 | 2021-10-10 | Fidia Farm Spa | Coniugato acido ialuronico-paclitaxel nel trattamento del mesotelioma |
IT202000008209A1 (it) | 2020-04-17 | 2021-10-17 | Fidia Farm Spa | Procedimento di sintesi di un coniugato acido ialuronico-paclitaxel |
CN111548978B (zh) * | 2020-05-14 | 2021-11-12 | 浙江农林大学 | 一种产甘露聚糖的枯草芽孢杆菌及其应用 |
IT202000014017A1 (it) | 2020-06-11 | 2021-12-11 | Fidia Farm Spa | Nuovi derivati ottenuti da acido ialuronico e carnosina |
IT202000032243A1 (it) | 2020-12-23 | 2022-06-23 | Fidia Farm Spa | Nuovi agenti antivirali |
CN113564154A (zh) * | 2021-08-16 | 2021-10-29 | 厦门大学 | 一种利用氧化石墨烯-羊毛角蛋白与金属离子配位固定化氧化还原酶的方法 |
IT202100032111A1 (it) | 2021-12-22 | 2023-06-22 | Fidia Farm Spa | Nuovi sostituti biocompatibili dell’umor vitreo |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9715660D0 (en) * | 1997-07-25 | 1997-10-01 | Zeneca Ltd | Proteins |
JP2005525091A (ja) * | 2001-12-21 | 2005-08-25 | ノボザイムス バイオポリマー アクティーゼルスカブ | 組換え宿主細胞におけるヒアルロナンの生成方法 |
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2010
- 2010-09-09 IT ITMI2010A001642A patent/IT1402385B1/it active
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2011
- 2011-09-09 WO PCT/EP2011/065642 patent/WO2012032154A1/en active Application Filing
- 2011-09-09 EP EP11764126.6A patent/EP2614088B1/en active Active
- 2011-09-09 ES ES11764126.6T patent/ES2593613T3/es active Active
- 2011-09-09 US US13/821,947 patent/US9290785B2/en active Active
-
2016
- 2016-02-17 US US15/045,704 patent/US10017802B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP2614088B1 (en) | 2016-08-31 |
US20130189740A1 (en) | 2013-07-25 |
IT1402385B1 (it) | 2013-09-04 |
US9290785B2 (en) | 2016-03-22 |
WO2012032154A1 (en) | 2012-03-15 |
ITMI20101642A1 (it) | 2012-03-10 |
EP2614088A1 (en) | 2013-07-17 |
US10017802B2 (en) | 2018-07-10 |
US20160237465A1 (en) | 2016-08-18 |
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