MX2021000201A - Sistema y métodos para modificar bacterias adecuadas para la producción, exportación y traducción de arnm eucariota en un hospedador eucariota. - Google Patents

Sistema y métodos para modificar bacterias adecuadas para la producción, exportación y traducción de arnm eucariota en un hospedador eucariota.

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Publication number
MX2021000201A
MX2021000201A MX2021000201A MX2021000201A MX2021000201A MX 2021000201 A MX2021000201 A MX 2021000201A MX 2021000201 A MX2021000201 A MX 2021000201A MX 2021000201 A MX2021000201 A MX 2021000201A MX 2021000201 A MX2021000201 A MX 2021000201A
Authority
MX
Mexico
Prior art keywords
eukaryotic
methods
export
translation
fit
Prior art date
Application number
MX2021000201A
Other languages
English (en)
Inventor
Richard Sayre
Pedro Costa-Nunes
Dr Guohua Yin
Original Assignee
Pebble Labs Usa Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pebble Labs Usa Inc filed Critical Pebble Labs Usa Inc
Publication of MX2021000201A publication Critical patent/MX2021000201A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La tecnología inventiva incluye sistemas, métodos y composiciones novedosos para generar organismos procariotas modificados genéticamente configurados para producir ARNm tipo eucariota que puede introducirse en un hospedador eucariota y traducirse a uno; los aspectos adicionales pueden incluir moléculas de ARNm y construcciones de nucleótidos tipo eucariota novedosas, así como métodos y sistemas para la administración eficaz de células procariotas a células hospedadores eucariotas diana; aún otros aspectos de la invención incluyen sistemas, métodos y composiciones para la transformación no integrativa de la célula eucariota.
MX2021000201A 2018-07-04 2019-07-05 Sistema y métodos para modificar bacterias adecuadas para la producción, exportación y traducción de arnm eucariota en un hospedador eucariota. MX2021000201A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862693963P 2018-07-04 2018-07-04
PCT/US2019/040747 WO2020010344A1 (en) 2018-07-04 2019-07-05 System and methods for engineering bacteria fit for eukaryotic mrna production, export, and translation in a eukaryotic host

Publications (1)

Publication Number Publication Date
MX2021000201A true MX2021000201A (es) 2021-06-08

Family

ID=69059973

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021000201A MX2021000201A (es) 2018-07-04 2019-07-05 Sistema y métodos para modificar bacterias adecuadas para la producción, exportación y traducción de arnm eucariota en un hospedador eucariota.

Country Status (10)

Country Link
US (1) US20210292775A1 (es)
EP (1) EP3818163A4 (es)
CN (1) CN113166768A (es)
AU (1) AU2019298340A1 (es)
CA (1) CA3105460A1 (es)
IL (1) IL279874A (es)
MX (1) MX2021000201A (es)
PH (1) PH12020552291A1 (es)
SG (1) SG11202013209SA (es)
WO (1) WO2020010344A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020245093A1 (en) * 2019-06-02 2020-12-10 Redbiotec Ag Bacterial delivery of gene editing tools into eukaryotic cells
CN115335527A (zh) * 2020-01-11 2022-11-11 西韦克生物技术有限责任公司 产生真核生物可翻译mRNA并将其传递给真核生物的微生物系统
WO2021173953A1 (en) * 2020-02-26 2021-09-02 Pebble Labs, Inc. Novel plant endophytic bacteria and methods to control plant pathogens and pests
CN117384867B (zh) * 2022-09-16 2024-06-07 北京普译生物科技有限公司 一种经修饰的Cas3移位酶及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1533438A (zh) * 2001-06-08 2004-09-29 �����Ŵ��Ƽ���˾ 在植物生产蛋白质的方法
US7663022B1 (en) * 2002-07-15 2010-02-16 Bruce Eric Hudkins Transgenic bioluminescent plants
AU2003256566A1 (en) * 2002-07-16 2004-02-02 Stuart Bussell Methods to construct multimeric dna and polymeric protein sequences as direct fusions or with linkers
EP3294878A1 (en) * 2015-05-15 2018-03-21 Pioneer Hi-Bred International, Inc. Guide rna/cas endonuclease systems
WO2017066845A1 (en) * 2015-10-23 2017-04-27 Queensland University Of Technology Organisms with modified growth and performance characteristics and methods of making them
US20190062734A1 (en) * 2016-04-13 2019-02-28 Editas Medicine, Inc. Grna fusion molecules, gene editing systems, and methods of use thereof
BR112019005183A2 (pt) * 2016-09-16 2019-07-02 Pebble Labs Usa Inc sistema paratransgênico inovador para o biocontrole de mosquitos transmissores de doença

Also Published As

Publication number Publication date
AU2019298340A1 (en) 2021-01-28
SG11202013209SA (en) 2021-01-28
EP3818163A4 (en) 2022-04-06
CA3105460A1 (en) 2020-01-09
CN113166768A (zh) 2021-07-23
WO2020010344A1 (en) 2020-01-09
IL279874A (en) 2021-03-01
US20210292775A1 (en) 2021-09-23
EP3818163A1 (en) 2021-05-12
PH12020552291A1 (en) 2021-06-28

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