ATE395424T1 - Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen - Google Patents

Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen

Info

Publication number
ATE395424T1
ATE395424T1 AT99925717T AT99925717T ATE395424T1 AT E395424 T1 ATE395424 T1 AT E395424T1 AT 99925717 T AT99925717 T AT 99925717T AT 99925717 T AT99925717 T AT 99925717T AT E395424 T1 ATE395424 T1 AT E395424T1
Authority
AT
Austria
Prior art keywords
mutant
nucleic acid
relates
trichothecene
cells
Prior art date
Application number
AT99925717T
Other languages
English (en)
Inventor
John Royer
Lynne Christianson
Gregory Gambetta
Howard Brody
Suzanne Otani
Wendy Yoder
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE395424T1 publication Critical patent/ATE395424T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT99925717T 1998-05-20 1999-05-20 Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen ATE395424T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8221798A 1998-05-20 1998-05-20

Publications (1)

Publication Number Publication Date
ATE395424T1 true ATE395424T1 (de) 2008-05-15

Family

ID=22169802

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99925717T ATE395424T1 (de) 1998-05-20 1999-05-20 Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen

Country Status (9)

Country Link
US (3) US6180366B1 (de)
EP (1) EP1078081B1 (de)
JP (2) JP2002515253A (de)
CN (2) CN1190495C (de)
AT (1) ATE395424T1 (de)
AU (1) AU4195099A (de)
DE (1) DE69938722D1 (de)
DK (1) DK1078081T3 (de)
WO (1) WO1999060137A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
US6214901B1 (en) * 1998-04-27 2001-04-10 Surmodics, Inc. Bioactive agent release coating
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
FR2826978B1 (fr) * 2001-07-06 2004-09-17 Agronomique Inst Nat Rech Procede de determination de la capacite de fusarium a produire des toxines trichotecenes
WO2003089617A2 (en) * 2002-04-22 2003-10-30 Novozymes Biotech, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
ATE439432T1 (de) 2002-08-30 2009-08-15 Novozymes Inc Verfahren zur herstellung von säugetier-trypsinen
WO2004020611A1 (en) * 2002-08-30 2004-03-11 Puratos Naamloze Vennootschap Myrothecium sp. transformation and expression system
AU2003294397A1 (en) 2002-11-18 2004-06-15 Novozymes Biotech, Inc. Promoter variants for expressing genes in a fungal cell
WO2007122249A1 (en) * 2006-04-25 2007-11-01 Dsm Ip Assets B.V. Production of compounds in a recombinant host
EP2026685A1 (de) * 2006-05-18 2009-02-25 Koninklijke Philips Electronics N.V. Horizontale toast-vorrichtung mit einer relativ kleinen oberen heizvorrichtung
WO2010039840A1 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
CN102695787A (zh) * 2009-08-05 2012-09-26 诺维信公司 用于从木素纤维素原料产生trichodiene的丝状真菌和方法
DK2513290T3 (en) 2009-12-18 2016-01-25 Novozymes Inc Methods for Preparation of Polypeptides in Trichoderma Mutants with Protease Deficiency
CN108588060B (zh) * 2017-03-07 2020-12-01 武汉康复得生物科技股份有限公司 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶
JP2021078354A (ja) * 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN112522231B (zh) * 2020-12-14 2023-03-14 广东省微生物研究所（广东省微生物分析检测中心） 一种酰基转移酶及其编码基因和应用
WO2023088791A1 (en) 2021-11-22 2023-05-25 Basf Se Paenibacillus strains producing low amounts of dab comprising compounds.

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
EP0777737B1 (de) * 1994-06-30 2005-05-04 Novozymes Biotech, Inc. Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
JP3628336B2 (ja) * 1996-03-11 2005-03-09 明治製菓株式会社 β―フルクトフラノシダーゼおよびその遺伝子、β―フルクトフラノシダーゼ遺伝子の単離法、β―フルクトフラノシダーゼの産生系、並びにβ―フルクトフラノシダーゼ変異体
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
US6383781B1 (en) * 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells

Also Published As

Publication number Publication date
JP2002515253A (ja) 2002-05-28
US7332341B2 (en) 2008-02-19
WO1999060137A2 (en) 1999-11-25
US20050181480A1 (en) 2005-08-18
US20080213901A1 (en) 2008-09-04
CN1190495C (zh) 2005-02-23
DK1078081T3 (da) 2008-08-04
CN1231579C (zh) 2005-12-14
EP1078081B1 (de) 2008-05-14
JP2009291207A (ja) 2009-12-17
CN1500882A (zh) 2004-06-02
AU4195099A (en) 1999-12-06
WO1999060137A3 (en) 2000-05-18
DE69938722D1 (de) 2008-06-26
CN1309711A (zh) 2001-08-22
EP1078081A2 (de) 2001-02-28
US6180366B1 (en) 2001-01-30

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