DE69938722D1 - Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen - Google Patents

Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen

Info

Publication number
DE69938722D1
DE69938722D1 DE69938722T DE69938722T DE69938722D1 DE 69938722 D1 DE69938722 D1 DE 69938722D1 DE 69938722 T DE69938722 T DE 69938722T DE 69938722 T DE69938722 T DE 69938722T DE 69938722 D1 DE69938722 D1 DE 69938722D1
Authority
DE
Germany
Prior art keywords
nucleic acid
mutant
relates
cells
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69938722T
Other languages
English (en)
Inventor
John C Royer
Lynne M Christianson
Gregory A Gambetta
Howard Brody
Suzanne M Otani
Wendy T Yoder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DE69938722D1 publication Critical patent/DE69938722D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69938722T 1998-05-20 1999-05-20 Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen Expired - Lifetime DE69938722D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8221798A 1998-05-20 1998-05-20
PCT/US1999/011223 WO1999060137A2 (en) 1998-05-20 1999-05-20 Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells

Publications (1)

Publication Number Publication Date
DE69938722D1 true DE69938722D1 (de) 2008-06-26

Family

ID=22169802

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69938722T Expired - Lifetime DE69938722D1 (de) 1998-05-20 1999-05-20 Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen

Country Status (9)

Country Link
US (3) US6180366B1 (de)
EP (1) EP1078081B1 (de)
JP (2) JP2002515253A (de)
CN (2) CN1190495C (de)
AT (1) ATE395424T1 (de)
AU (1) AU4195099A (de)
DE (1) DE69938722D1 (de)
DK (1) DK1078081T3 (de)
WO (1) WO1999060137A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
AU760408B2 (en) * 1998-04-27 2003-05-15 Surmodics, Inc. Bioactive agent release coating
DK1078081T3 (da) * 1998-05-20 2008-08-04 Novozymes Inc Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller
FR2826978B1 (fr) * 2001-07-06 2004-09-17 Agronomique Inst Nat Rech Procede de determination de la capacite de fusarium a produire des toxines trichotecenes
WO2003089617A2 (en) * 2002-04-22 2003-10-30 Novozymes Biotech, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
US20060240509A1 (en) * 2002-08-30 2006-10-26 Jean-Luc Jonniaux Myrothecium sp transformation and expression system
AU2003273256A1 (en) * 2002-08-30 2004-03-19 Novozymes Biotech, Inc. Methods for producing mammalian trypsins
JP4490284B2 (ja) 2002-11-18 2010-06-23 ノボザイムス，インコーポレイティド 菌類細胞において遺伝子を発現するためのプロモーター変異体
US20090233287A1 (en) * 2006-04-25 2009-09-17 Roelof Ary Lans Bovenberg Production of compounds in a recombinant host
US20090223380A1 (en) * 2006-05-18 2009-09-10 Koninklijke Philips Electronics N.V. Horizontal toasting device having relatively small top heating means
US8647856B2 (en) 2008-09-30 2014-02-11 Novozymes Inc. Methods for producing polypeptides in enzyme-deficient mutants of Fusarium venenatum
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
US20120184008A1 (en) * 2009-08-05 2012-07-19 Novozymes A/S Filamentous fungi and methods for producing trichodiene from lignocellulosic feedstocks
BR122018077153B1 (pt) 2009-12-18 2020-10-27 Novozymes, Inc mutante de uma cepa de trichoderma reesei parental,e, métodos para produzir um polipeptídeo de interesse e para obter um mutante de uma cepa de trichoderma reesei parental
CN108588060B (zh) * 2017-03-07 2020-12-01 武汉康复得生物科技股份有限公司 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶
JP2021078354A (ja) * 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN112522231B (zh) * 2020-12-14 2023-03-14 广东省微生物研究所（广东省微生物分析检测中心） 一种酰基转移酶及其编码基因和应用
WO2023088791A1 (en) 2021-11-22 2023-05-25 Basf Se Paenibacillus strains producing low amounts of dab comprising compounds.

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
AU2705895A (en) * 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
WO1997034004A1 (fr) * 1996-03-11 1997-09-18 Meiji Seika Kaisha, Ltd. β-FRUCTOFURANNOSIDASE ET SON GENE, PROCEDE D'ISOLEMENT DU GENE DE β-FRUCTOFURANNOSIDASE, SYSTEME POUR LA PRODUCTION DE β-FRUCTOFURANNOSIDASE, ET VARIANT DE β-FRUCTOFURANNOSIDASE
DK1078081T3 (da) * 1998-05-20 2008-08-04 Novozymes Inc Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller
US6383781B1 (en) * 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells

Also Published As

Publication number Publication date
JP2002515253A (ja) 2002-05-28
CN1190495C (zh) 2005-02-23
CN1500882A (zh) 2004-06-02
DK1078081T3 (da) 2008-08-04
WO1999060137A2 (en) 1999-11-25
EP1078081A2 (de) 2001-02-28
US20080213901A1 (en) 2008-09-04
AU4195099A (en) 1999-12-06
WO1999060137A3 (en) 2000-05-18
CN1231579C (zh) 2005-12-14
JP2009291207A (ja) 2009-12-17
ATE395424T1 (de) 2008-05-15
EP1078081B1 (de) 2008-05-14
US20050181480A1 (en) 2005-08-18
US7332341B2 (en) 2008-02-19
CN1309711A (zh) 2001-08-22
US6180366B1 (en) 2001-01-30

Similar Documents

Publication Publication Date Title
DE69939947D1 (de) Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
DE69938722D1 (de) Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
WO2000056900A3 (en) Promoter sequences derived from fusarium venenatum and uses thereof
ATE502115T1 (de) Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle
DE69723855D1 (de) Verfahren zur herstellung von polypeptiden in surfaktinmutanten von bacillus zellen
AU2912301A (en) Pyrophosphatase stress-related proteins and methods of use in plants
RU2006117757A (ru) Высокоэффективный перенос и экспрессия генов в клетках млекопитающих путем процедуры множественной трансфекции последовательностей mar
DE69720026D1 (de) Verfahren zur herstellung von d-aminosäuren durch direkte gärung
DE602004028724D1 (de) Verfahren zur herstellung sezernierter polypeptide
AU2003294397A1 (en) Promoter variants for expressing genes in a fungal cell
ATE353953T1 (de) Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion
DE60115225D1 (de) Verfahren zur bestimmung einer genfunktion
CN112430586B (zh) 一种VI-B型CRISPR/Cas13基因编辑系统及其应用
WO2000046375A3 (en) Methods for producing polypeptides in fungal cells
EP2281892A3 (de) Verfahren zur Herstellung von Polypeptiden in atmungsdefizienten Trichoderma Zellen
CA2559093A1 (en) Proteins imparting boron-tolerance and genes thereof
DE60226592D1 (de) Desoxycytidinkinase-mutanten mit erweiterter enzymaktivität
WO2000042203A3 (en) Methods for producing polypeptides in cyclohexadepsipeptide-deficient cells
ATE451469T1 (de) In vivo-verfahren zur herstellung von proteinen, die nicht-konventionelle aminosäuren enthalten
KR20050025180A (ko) 메틸트로픽 효모로부터 유도된 변화된 전사 효율을 갖는프로모터
WO2001051646A3 (en) Methods for producing a polypeptide using a crippled translational initiator sequence
DE50211058D1 (de) Verfahren zur herstellung von arachidonsäure in transgenen organismen
Royer et al. Methods for producing heterologous polypeptides in trichothecene-deficient fungi
DE60013829D1 (en) Pichia methanolica glyceraldehyd-3-phosphat-dehydrogenase-1-promoter und -terminator
DE69736520D1 (de) Modifikation von kryptischen splicemotiven heterologer gene zur expression in pilzen

Legal Events

Date Code Title Description
8364 No opposition during term of opposition