DK1572895T3 - Fremgangsmåder til fremstilling af hyaluronan i en rekombinant værtscelle - Google Patents

Fremgangsmåder til fremstilling af hyaluronan i en rekombinant værtscelle

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Publication number
DK1572895T3
DK1572895T3 DK02805661.2T DK02805661T DK1572895T3 DK 1572895 T3 DK1572895 T3 DK 1572895T3 DK 02805661 T DK02805661 T DK 02805661T DK 1572895 T3 DK1572895 T3 DK 1572895T3
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DK
Denmark
Prior art keywords
udp
glucose
gene
host cell
hyaluronan synthase
Prior art date
Application number
DK02805661.2T
Other languages
English (en)
Inventor
Alan Sloma
Regine Behr
William Widner
Maria Tang
David Sternberg
Stephen Brown
Original Assignee
Novozymes Biopharma Dk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biopharma Dk As filed Critical Novozymes Biopharma Dk As
Application granted granted Critical
Publication of DK1572895T3 publication Critical patent/DK1572895T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
DK02805661.2T 2001-12-21 2002-12-20 Fremgangsmåder til fremstilling af hyaluronan i en rekombinant værtscelle DK1572895T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34264401P 2001-12-21 2001-12-21
PCT/US2002/041067 WO2003054163A2 (en) 2001-12-21 2002-12-20 Methods for producing hyaluronan in a recombinant host cell

Publications (1)

Publication Number Publication Date
DK1572895T3 true DK1572895T3 (da) 2011-07-11

Family

ID=23342668

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02805661.2T DK1572895T3 (da) 2001-12-21 2002-12-20 Fremgangsmåder til fremstilling af hyaluronan i en rekombinant værtscelle

Country Status (18)

Country Link
US (5) US7811806B2 (da)
EP (1) EP1572895B1 (da)
JP (1) JP2005525091A (da)
KR (2) KR100885163B1 (da)
CN (1) CN1636052B (da)
AT (1) ATE502115T1 (da)
AU (1) AU2002366711C1 (da)
BR (1) BR0215220A (da)
CA (2) CA2803931A1 (da)
CZ (1) CZ2004765A3 (da)
DE (1) DE60239495D1 (da)
DK (1) DK1572895T3 (da)
ES (1) ES2362354T3 (da)
HU (2) HU228693B1 (da)
IL (2) IL162302A0 (da)
PL (2) PL399352A1 (da)
RU (1) RU2346049C2 (da)
WO (1) WO2003054163A2 (da)

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AU2002365206B2 (en) * 2001-06-13 2008-03-20 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof
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US7002007B2 (en) * 2004-05-28 2006-02-21 Calcigen Corporation Production of high molecular weight hyaluronates
CN101006174B (zh) 2004-06-21 2010-05-26 诺维信公司 以相同取向稳定保持的至少两个orf的多拷贝
SI1805312T1 (sl) 2004-09-23 2009-12-31 Bayer Cropscience Ag Postopki in sredstva za izdelavo hialuronana
EP1640457A1 (de) * 2004-09-23 2006-03-29 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan
PL1817347T3 (pl) * 2004-11-24 2017-10-31 Albumedix As Sposób sieciowania kwasu hialuronowego za pomocą diwinylosulfonu
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EP1696032A1 (de) * 2005-02-23 2006-08-30 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan in Pilzen
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
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US20080038780A1 (en) * 2006-02-15 2008-02-14 Novozymes Biopolymer A/S Production of low molecular weight hyaluronic acid
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JP2009545637A (ja) 2006-08-04 2009-12-24 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 分岐ヒアルロン酸及びその製造方法
ATE533838T1 (de) 2006-11-29 2011-12-15 Novozymes Inc Verfahren zur verbesserten einführung von dna in bakterienzellen
DK2104739T3 (da) 2006-12-21 2013-10-07 Novozymes Inc Modificerede messenger-RNA-stabiliseringssekvenser til ekspression af gener i bakterieceller
US20110281817A1 (en) * 2007-08-31 2011-11-17 Sugar Industry Innovation Pty. Ltd. Production of hyaluronic acid
EP2031053A1 (en) * 2007-08-31 2009-03-04 The University Of Queensland Production of HA
EP2036983A1 (de) 2007-09-12 2009-03-18 Bayer CropScience AG Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren
CN101878230B (zh) 2007-12-19 2012-11-21 赢创高施米特有限公司 乳液中的交联透明质酸
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CN102021213B (zh) * 2009-09-17 2014-07-09 上海佰加壹医药有限公司 一种发酵生产透明质酸发酵液的方法
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IT1402384B1 (it) 2010-09-09 2013-09-04 Fidia Farmaceutici Processo per la produzione di acido ialuronico in escherichia coli o bacillus megaterium
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CN103562229A (zh) 2011-05-30 2014-02-05 诺维信生物制药丹麦公司 高分子量透明质酸的喷雾干燥
CN102911887A (zh) * 2011-08-02 2013-02-06 山东省生物药物研究院 一种利用毕赤酵母基因调控发酵生产不同分子量透明质酸的方法
KR20140058581A (ko) 2011-09-02 2014-05-14 노보자임스 바이오파마 디케이 에이/에스 지연된 약물 방출용 히알루론산 함유 경구 제형
DE102012201297A1 (de) * 2012-01-31 2013-08-01 Basf Se Expressionsverfahren
CN102559559A (zh) * 2012-02-21 2012-07-11 山东福瑞达生物医药有限公司 一种芽孢杆菌及采用该菌种生产透明质酸酶的方法
WO2013123791A1 (zh) * 2012-02-21 2013-08-29 华熙福瑞达生物医药有限公司 一种芽孢杆菌、一种透明质酸酶、以及其用途
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CA2896038C (en) 2015-07-03 2022-08-09 Glycobiosciences Inc. Polymer matrix compositions comprising a high concentration of bio-fermented sodium hyaluronate and uses thereof
CN106755022B (zh) * 2015-11-25 2020-08-04 中国科学院大连化学物理研究所 乙酰葡萄糖胺磷酸变位酶AtAGM编码基因及其酶、制备、应用与酶活性检测方法
CN109715794A (zh) 2016-07-05 2019-05-03 诺维信公司 果胶裂解酶变体以及编码它们的多核苷酸
CN107653280B (zh) * 2016-07-25 2021-05-04 安琪酵母股份有限公司 透明质酸发酵用的组合物、培养基和方法及其应用
CN106381279B (zh) * 2016-08-29 2019-11-05 中国药科大学 一种细菌胞外多糖、其制备方法及其应用
KR102135044B1 (ko) * 2018-12-10 2020-07-17 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
WO2020122429A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
WO2020122430A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 비병원성 세균을 이용하여 히알루론산 생산을 위한 발현 시스템 및 상기 발현 시스템을 이용한 히알루론산 생산방법
CN110055201B (zh) * 2019-03-05 2021-05-28 江南大学 一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法
RU2723722C1 (ru) * 2019-07-26 2020-06-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ
RU2719140C1 (ru) 2019-07-26 2020-04-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии
CN111518825B (zh) * 2020-04-30 2022-10-11 浙江工业大学 一种多基因组合表达制备蛹虫草多糖的方法
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Also Published As

Publication number Publication date
US8137951B2 (en) 2012-03-20
AU2002366711A1 (en) 2003-07-09
RU2004122420A (ru) 2005-10-10
JP2005525091A (ja) 2005-08-25
ATE502115T1 (de) 2011-04-15
IL162302A0 (en) 2005-11-20
DE60239495D1 (de) 2011-04-28
US20110189737A1 (en) 2011-08-04
US20110014662A1 (en) 2011-01-20
US20030175902A1 (en) 2003-09-18
CN1636052B (zh) 2014-07-02
KR100879908B1 (ko) 2009-01-21
AU2002366711C1 (en) 2009-01-22
CN1636052A (zh) 2005-07-06
CA2471148C (en) 2013-04-09
WO2003054163A2 (en) 2003-07-03
EP1572895A2 (en) 2005-09-14
PL399352A1 (pl) 2012-10-08
US8574886B2 (en) 2013-11-05
RU2346049C2 (ru) 2009-02-10
HU228693B1 (hu) 2013-05-28
WO2003054163A3 (en) 2005-10-27
IL162302A (en) 2011-01-31
KR20080018965A (ko) 2008-02-28
US8093036B2 (en) 2012-01-10
HU1300136D0 (hu) 2007-05-29
CA2471148A1 (en) 2003-07-03
ES2362354T3 (es) 2011-07-04
AU2002366711B2 (en) 2007-11-29
EP1572895A4 (en) 2007-07-25
CA2803931A1 (en) 2003-07-03
PL375190A1 (en) 2005-11-28
KR20040085142A (ko) 2004-10-07
PL212928B1 (pl) 2012-12-31
KR100885163B1 (ko) 2009-02-23
US20140038235A1 (en) 2014-02-06
HUP0700154A2 (en) 2007-05-29
CZ2004765A3 (cs) 2004-12-15
US20120149067A1 (en) 2012-06-14
EP1572895B1 (en) 2011-03-16
US7811806B2 (en) 2010-10-12
BR0215220A (pt) 2007-01-09

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