BR0215220A - método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isolado - Google Patents
método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isoladoInfo
- Publication number
- BR0215220A BR0215220A BRPI0215220-7A BR0215220A BR0215220A BR 0215220 A BR0215220 A BR 0215220A BR 0215220 A BR0215220 A BR 0215220A BR 0215220 A BR0215220 A BR 0215220A
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- producing
- host cell
- udp
- expression vector
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title abstract 4
- 241000193830 Bacillus <bacterium> Species 0.000 title abstract 4
- 229920002674 hyaluronan Polymers 0.000 title abstract 4
- 229960003160 hyaluronic acid Drugs 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 239000013604 expression vector Substances 0.000 title abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 title 2
- 102000004196 processed proteins & peptides Human genes 0.000 title 2
- 108090000765 processed proteins & peptides Proteins 0.000 title 2
- 238000003259 recombinant expression Methods 0.000 title 1
- 108090000320 Hyaluronan Synthases Proteins 0.000 abstract 4
- 102000003918 Hyaluronan Synthases Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 101710167959 Putative UTP-glucose-1-phosphate uridylyltransferase Proteins 0.000 abstract 2
- 102000048175 UTP-glucose-1-phosphate uridylyltransferases Human genes 0.000 abstract 2
- 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 abstract 1
- 108030001662 UDP-glucose 6-dehydrogenases Proteins 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 238000010276 construction Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
"MéTODO PARA PRODUZIR UM áCIDO HIALURÈNICO, CéLULA HOSPEDEIRA DE BACILLUS, CONSTRUçãO DE áCIDO NUCLéICO SEQüêNCIA DE áCIDO NUCLéICO ISOLADA, VETOR DE EXPRESSãO, VETOR DE EXPRESSãO RECOMBINANTE, CéLULA HOSPEDEIRA RECOMBINANTE, MéTODO PARA PRODUZIR UM POLIPEPTìDEO, E, POLIPEPTìDEO ISOLADO". A presente invenção diz respeito a métodos para produzir ácido hialurónico, compreendendo: (a) cultivar uma célula hospedeira de Bacillus sob condições adequadas para a produção do ácido hialurónico, em que a célula hospedeira de Bacillus compreende uma construção de ácido nucléico compreendendo uma seqüência codificando a hialuronano sintase operavelmente ligada a uma seqüência promotora estranha à seqüência codificando a hialuronano sintase; e (b) recuperar o ácido hialurónico do meio de cultivo. A presente invenção também diz respeito a uma seqüência de ácido nucléico isolada codificando um óperon de hialuronano sintase que compreende um gene de hialuronano sintase e um gene de UDP-glicose 6-desidrogenase, e opcionalmente um ou mais genes selecionados do grupo consistindo de um gene de UDP-glicose pirofosforilase, um gene de UDP-Nacetilglicosamina pirofosforilase, e um gene de glicose-6-fosfato isomerase. A presente invenção também diz respeito a seqüências de ácido nucléico isoladas codificando uma UDP-glicose-6-desidrocenase, UDP-glicose pirofosforilase, e UDP-N-acetilglicosamina pirofosforilase.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34264401P | 2001-12-21 | 2001-12-21 | |
| PCT/US2002/041067 WO2003054163A2 (en) | 2001-12-21 | 2002-12-20 | Methods for producing hyaluronan in a recombinant host cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0215220A true BR0215220A (pt) | 2007-01-09 |
Family
ID=23342668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0215220-7A BR0215220A (pt) | 2001-12-21 | 2002-12-20 | método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isolado |
Country Status (18)
| Country | Link |
|---|---|
| US (5) | US7811806B2 (pt) |
| EP (1) | EP1572895B1 (pt) |
| JP (1) | JP2005525091A (pt) |
| KR (2) | KR100885163B1 (pt) |
| CN (1) | CN1636052B (pt) |
| AT (1) | ATE502115T1 (pt) |
| AU (1) | AU2002366711C1 (pt) |
| BR (1) | BR0215220A (pt) |
| CA (2) | CA2803931A1 (pt) |
| CZ (1) | CZ2004765A3 (pt) |
| DE (1) | DE60239495D1 (pt) |
| DK (1) | DK1572895T3 (pt) |
| ES (1) | ES2362354T3 (pt) |
| HU (2) | HU228693B1 (pt) |
| IL (2) | IL162302A0 (pt) |
| PL (2) | PL212928B1 (pt) |
| RU (1) | RU2346049C2 (pt) |
| WO (1) | WO2003054163A2 (pt) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7091008B1 (en) * | 1994-07-01 | 2006-08-15 | The Board Of Regents Of The University Of Oklahoma | Hyaluronan synthase genes and expression thereof in Bacillus hosts |
| EP1025211B1 (en) * | 1997-10-31 | 2004-12-15 | The Board of Regents of The University of Oklahoma | Hyaluronan synthase gene and uses thereof |
| CN1620511A (zh) * | 2001-06-13 | 2005-05-25 | 俄克拉何马大学董事会 | 透明质素合成酶基因及其表达 |
| KR100885163B1 (ko) * | 2001-12-21 | 2009-02-23 | 노보자임스 바이오폴리머 에이/에스 | 재조합체 숙주 세포에서 히알루로난의 제조 방법 |
| ES2359381T5 (es) * | 2002-05-14 | 2014-07-10 | Novozymes A/S | Variantes de pectato liasa |
| KR101023049B1 (ko) * | 2002-06-20 | 2011-03-24 | 노보자임스 바이오파마 디케이 에이/에스 | 2가 염을 사용한 응집 방법 |
| DK1537229T3 (da) * | 2002-07-26 | 2013-12-02 | Novozymes Inc | Fremgangsmåder til produktion af biologiske stoffer i pigmentmanglende mutanter af Bacillus-celler |
| EP3018140A3 (en) * | 2004-01-09 | 2016-08-17 | Basf Se | Inactivation of glutamylpolypeptide synthesis in bacillus |
| EP1735455A2 (en) * | 2004-03-31 | 2006-12-27 | Novozymes Biopolymer A/S | Methods for producing hyaluronic acid in a bacillus cell |
| US7002007B2 (en) * | 2004-05-28 | 2006-02-21 | Calcigen Corporation | Production of high molecular weight hyaluronates |
| US20080044853A1 (en) | 2004-06-21 | 2008-02-21 | Novozymes A/S | Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation |
| EP1640457A1 (de) * | 2004-09-23 | 2006-03-29 | Bayer CropScience GmbH | Verfahren und Mittel zur Herstellung von Hyaluronan |
| SI1805312T1 (sl) | 2004-09-23 | 2009-12-31 | Bayer Cropscience Ag | Postopki in sredstva za izdelavo hialuronana |
| PL1817347T3 (pl) * | 2004-11-24 | 2017-10-31 | Albumedix As | Sposób sieciowania kwasu hialuronowego za pomocą diwinylosulfonu |
| EP1833882A1 (en) * | 2004-12-30 | 2007-09-19 | Novozymes Biopolymer A/S | Hyaluronic acid linked with a polymer of an alpha hydroxy acid |
| EP1696032A1 (de) * | 2005-02-23 | 2006-08-30 | Bayer CropScience GmbH | Verfahren und Mittel zur Herstellung von Hyaluronan in Pilzen |
| JP4639904B2 (ja) | 2005-03-30 | 2011-02-23 | チッソ株式会社 | 蛍光活性を有するルシフェラーゼの活性増強方法 |
| JP4609177B2 (ja) | 2005-04-28 | 2011-01-12 | チッソ株式会社 | カルシウム結合型発光蛋白質溶液の発光時間延長方法 |
| US20060287512A1 (en) * | 2005-05-04 | 2006-12-21 | Novozymes Biopolymer A/S | Method of controlling the content of selected component(s) from polymer(s) using molecular sieve(s) |
| BRPI0614885A2 (pt) * | 2005-08-25 | 2011-04-19 | Toyo Boseki | planta produtora de ácido hialurÈnico |
| EP1951878B1 (en) * | 2005-10-05 | 2015-02-25 | Bayer Intellectual Property GmbH | Plants with increased hyaluronan production |
| EP1772052A1 (de) | 2005-10-05 | 2007-04-11 | Bayer CropScience GmbH | Verbesserte Verfahren und Mittel zur Herstellung von Hyaluronan |
| JP2007174957A (ja) * | 2005-12-27 | 2007-07-12 | Toyobo Co Ltd | ヒアルロン酸生産酵母 |
| US20080038780A1 (en) | 2006-02-15 | 2008-02-14 | Novozymes Biopolymer A/S | Production of low molecular weight hyaluronic acid |
| BRPI0708776A2 (pt) * | 2006-03-14 | 2011-06-14 | Novozymes Biopolymer As | produto de Ácido hialurânico acrilado, e, processo para preparaÇço e mÉtodo para polimerizaÇço/reticulaÇço do mesmo |
| AU2007280846B2 (en) | 2006-08-04 | 2012-06-14 | Novozymes Biopharma Dk A/S | Branched hyaluronic acid and method of manufacture |
| ATE533838T1 (de) | 2006-11-29 | 2011-12-15 | Novozymes Inc | Verfahren zur verbesserten einführung von dna in bakterienzellen |
| CN101611145B (zh) | 2006-12-21 | 2013-08-14 | 诺维信股份有限公司 | 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列 |
| EP2031053A1 (en) * | 2007-08-31 | 2009-03-04 | The University Of Queensland | Production of HA |
| US20110281817A1 (en) * | 2007-08-31 | 2011-11-17 | Sugar Industry Innovation Pty. Ltd. | Production of hyaluronic acid |
| EP2036983A1 (de) * | 2007-09-12 | 2009-03-18 | Bayer CropScience AG | Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren |
| JP5523338B2 (ja) | 2007-12-19 | 2014-06-18 | エヴォニク ゴールドシュミット ゲーエムベーハー | エマルジョン中の架橋ヒアルロン酸 |
| WO2010068955A2 (en) | 2008-12-13 | 2010-06-17 | Dna Microarray | MICROENVIRONMENT NICHE ASSAY FOR CiPS SCREENING |
| EP2213315A1 (en) | 2009-01-30 | 2010-08-04 | Mero S.r.L. | Antibacterial hydrogel and use thereof in orthopedics |
| CN102021213B (zh) * | 2009-09-17 | 2014-07-09 | 上海佰加壹医药有限公司 | 一种发酵生产透明质酸发酵液的方法 |
| CN102884182A (zh) | 2010-03-03 | 2013-01-16 | 诺维信股份有限公司 | 木聚糖酶变体及编码其的多核苷酸 |
| IT1401498B1 (it) | 2010-07-30 | 2013-07-26 | Mero Srl | Idrogelo a base di acido ialuronico e suo uso in ortopedia |
| IT1402385B1 (it) * | 2010-09-09 | 2013-09-04 | Fidia Farmaceutici | Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis |
| IT1402384B1 (it) | 2010-09-09 | 2013-09-04 | Fidia Farmaceutici | Processo per la produzione di acido ialuronico in escherichia coli o bacillus megaterium |
| CA2835016A1 (en) | 2011-05-30 | 2012-12-06 | Novozymes Biopharma Dk A/S | Spray drying of high molecular weight hyaluronic acid |
| CN102911887A (zh) * | 2011-08-02 | 2013-02-06 | 山东省生物药物研究院 | 一种利用毕赤酵母基因调控发酵生产不同分子量透明质酸的方法 |
| CA2847328A1 (en) | 2011-09-02 | 2013-03-07 | Novozymes Biopharma Dk A/S | Oral formulations containing hyaluronic acid for sustained drug release |
| DE102012201297A1 (de) * | 2012-01-31 | 2013-08-01 | Basf Se | Expressionsverfahren |
| CN102559559A (zh) * | 2012-02-21 | 2012-07-11 | 山东福瑞达生物医药有限公司 | 一种芽孢杆菌及采用该菌种生产透明质酸酶的方法 |
| US9493755B2 (en) * | 2012-02-21 | 2016-11-15 | Bloomage Freda Biopharm Co., Ltd. | Bacillus, hyaluronidase, and uses thereof |
| JP6306519B2 (ja) * | 2012-02-22 | 2018-04-04 | ノボザイムス アクティーゼルスカブ | 高度発酵制御 |
| WO2014100837A1 (en) | 2012-12-17 | 2014-06-26 | Bui The Duy | Computer aided implantation of body implants |
| CN105873955B (zh) | 2013-11-25 | 2019-03-15 | 杜特里亚生物材料有限责任公司 | 富氘透明质酸 |
| CA2896038C (en) | 2015-07-03 | 2022-08-09 | Glycobiosciences Inc. | Polymer matrix compositions comprising a high concentration of bio-fermented sodium hyaluronate and uses thereof |
| CN106755022B (zh) * | 2015-11-25 | 2020-08-04 | 中国科学院大连化学物理研究所 | 乙酰葡萄糖胺磷酸变位酶AtAGM编码基因及其酶、制备、应用与酶活性检测方法 |
| WO2018007435A1 (en) | 2016-07-05 | 2018-01-11 | Novozymes A/S | Pectate lyase variants and polynucleotides encoding same |
| CN107653280B (zh) * | 2016-07-25 | 2021-05-04 | 安琪酵母股份有限公司 | 透明质酸发酵用的组合物、培养基和方法及其应用 |
| CN106381279B (zh) * | 2016-08-29 | 2019-11-05 | 中国药科大学 | 一种细菌胞外多糖、其制备方法及其应用 |
| WO2020122429A1 (ko) * | 2018-12-10 | 2020-06-18 | 대화제약 주식회사 | 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법 |
| KR102135044B1 (ko) * | 2018-12-10 | 2020-07-17 | 대화제약 주식회사 | 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법 |
| WO2020122430A1 (ko) * | 2018-12-10 | 2020-06-18 | 대화제약 주식회사 | 비병원성 세균을 이용하여 히알루론산 생산을 위한 발현 시스템 및 상기 발현 시스템을 이용한 히알루론산 생산방법 |
| CN110055201B (zh) * | 2019-03-05 | 2021-05-28 | 江南大学 | 一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法 |
| RU2723722C1 (ru) * | 2019-07-26 | 2020-06-17 | Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" | ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ |
| RU2719140C1 (ru) | 2019-07-26 | 2020-04-17 | Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" | Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии |
| CN111518825B (zh) * | 2020-04-30 | 2022-10-11 | 浙江工业大学 | 一种多基因组合表达制备蛹虫草多糖的方法 |
| EP4294823A1 (en) * | 2021-02-22 | 2023-12-27 | Danisco US Inc. | Methods and compositions for producing proteins of interest in pigment deficient bacillus cells |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60251898A (ja) * | 1984-05-25 | 1985-12-12 | Shiseido Co Ltd | 醗酵法によるヒアルロン酸の製造方法 |
| US6455304B1 (en) | 1994-07-01 | 2002-09-24 | The Board Of Regents Of The University Of Oklahoma | Hyaluronate synthase gene and uses thereof |
| US6951743B2 (en) | 1997-10-31 | 2005-10-04 | University Of Oklahoma Board Of Regents | Hyaluronan synthase genes and expression thereof in bacillus hosts |
| IT1271001B (it) | 1994-07-26 | 1997-05-26 | Poli Ind Chimica Spa | Procedimento di preparazione di acido ialuronico mediante fermentazione con streptococcus |
| EP1025211B1 (en) | 1997-10-31 | 2004-12-15 | The Board of Regents of The University of Oklahoma | Hyaluronan synthase gene and uses thereof |
| US5955310A (en) * | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| BR9909346A (pt) * | 1998-04-02 | 2001-09-11 | Univ Oklahoma | Segmento de ácido nucleico, vetor recombinante, célula hospedeira, composição, processos para detectar uma espécie de dna, uma célula bacteriana e infecção, para produzir ácido hialurÈnico e para criar uma vacina, sequência de ácido nucleico e de cdna, vacina e teste diagnóstico |
| EP1129209B1 (en) | 1998-11-11 | 2009-01-21 | The Board Of Regents Of The University Of Oklahoma | Polymer grafting by polysaccharide synthases |
| WO2002077183A2 (en) | 2001-03-21 | 2002-10-03 | Elitra Pharmaceuticals, Inc. | Identification of essential genes in microorganisms |
| CN1620511A (zh) * | 2001-06-13 | 2005-05-25 | 俄克拉何马大学董事会 | 透明质素合成酶基因及其表达 |
| KR100885163B1 (ko) * | 2001-12-21 | 2009-02-23 | 노보자임스 바이오폴리머 에이/에스 | 재조합체 숙주 세포에서 히알루로난의 제조 방법 |
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