BR0215220A - método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isolado - Google Patents

método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isolado

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Publication number
BR0215220A
BR0215220A BRPI0215220-7A BR0215220A BR0215220A BR 0215220 A BR0215220 A BR 0215220A BR 0215220 A BR0215220 A BR 0215220A BR 0215220 A BR0215220 A BR 0215220A
Authority
BR
Brazil
Prior art keywords
nucleic acid
producing
host cell
udp
expression vector
Prior art date
Application number
BRPI0215220-7A
Other languages
English (en)
Inventor
Alan Sloma
Regine Behr
William Widner
Maria Tang
David Sternberg
Stephen Brown
Original Assignee
Novozymes Biopolymer As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biopolymer As filed Critical Novozymes Biopolymer As
Publication of BR0215220A publication Critical patent/BR0215220A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Abstract

"MéTODO PARA PRODUZIR UM áCIDO HIALURÈNICO, CéLULA HOSPEDEIRA DE BACILLUS, CONSTRUçãO DE áCIDO NUCLéICO SEQüêNCIA DE áCIDO NUCLéICO ISOLADA, VETOR DE EXPRESSãO, VETOR DE EXPRESSãO RECOMBINANTE, CéLULA HOSPEDEIRA RECOMBINANTE, MéTODO PARA PRODUZIR UM POLIPEPTìDEO, E, POLIPEPTìDEO ISOLADO". A presente invenção diz respeito a métodos para produzir ácido hialurónico, compreendendo: (a) cultivar uma célula hospedeira de Bacillus sob condições adequadas para a produção do ácido hialurónico, em que a célula hospedeira de Bacillus compreende uma construção de ácido nucléico compreendendo uma seqüência codificando a hialuronano sintase operavelmente ligada a uma seqüência promotora estranha à seqüência codificando a hialuronano sintase; e (b) recuperar o ácido hialurónico do meio de cultivo. A presente invenção também diz respeito a uma seqüência de ácido nucléico isolada codificando um óperon de hialuronano sintase que compreende um gene de hialuronano sintase e um gene de UDP-glicose 6-desidrogenase, e opcionalmente um ou mais genes selecionados do grupo consistindo de um gene de UDP-glicose pirofosforilase, um gene de UDP-Nacetilglicosamina pirofosforilase, e um gene de glicose-6-fosfato isomerase. A presente invenção também diz respeito a seqüências de ácido nucléico isoladas codificando uma UDP-glicose-6-desidrocenase, UDP-glicose pirofosforilase, e UDP-N-acetilglicosamina pirofosforilase.
BRPI0215220-7A 2001-12-21 2002-12-20 método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isolado BR0215220A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34264401P 2001-12-21 2001-12-21
PCT/US2002/041067 WO2003054163A2 (en) 2001-12-21 2002-12-20 Methods for producing hyaluronan in a recombinant host cell

Publications (1)

Publication Number Publication Date
BR0215220A true BR0215220A (pt) 2007-01-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0215220-7A BR0215220A (pt) 2001-12-21 2002-12-20 método para produzir um ácido hialurÈnico, célula hospedeira de bacillus, construção de ácido nucléico, seqüência de ácido nucléico isolada, vetor de expressão , vetor de expressão recombinante, célula hospedeira recombinante, método para produzir um polipeptìdeo, e, polipeptìdeo isolado

Country Status (18)

Country Link
US (5) US7811806B2 (pt)
EP (1) EP1572895B1 (pt)
JP (1) JP2005525091A (pt)
KR (2) KR100885163B1 (pt)
CN (1) CN1636052B (pt)
AT (1) ATE502115T1 (pt)
AU (1) AU2002366711C1 (pt)
BR (1) BR0215220A (pt)
CA (2) CA2471148C (pt)
CZ (1) CZ2004765A3 (pt)
DE (1) DE60239495D1 (pt)
DK (1) DK1572895T3 (pt)
ES (1) ES2362354T3 (pt)
HU (2) HU228693B1 (pt)
IL (2) IL162302A0 (pt)
PL (2) PL399352A1 (pt)
RU (1) RU2346049C2 (pt)
WO (1) WO2003054163A2 (pt)

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WO2003054163A3 (en) 2005-10-27
US20110189737A1 (en) 2011-08-04
PL212928B1 (pl) 2012-12-31
PL399352A1 (pl) 2012-10-08
AU2002366711A1 (en) 2003-07-09
US8137951B2 (en) 2012-03-20
CN1636052B (zh) 2014-07-02
KR20080018965A (ko) 2008-02-28
AU2002366711C1 (en) 2009-01-22
CZ2004765A3 (cs) 2004-12-15
PL375190A1 (en) 2005-11-28
KR20040085142A (ko) 2004-10-07
EP1572895A2 (en) 2005-09-14
JP2005525091A (ja) 2005-08-25
ES2362354T3 (es) 2011-07-04
AU2002366711B2 (en) 2007-11-29
US20120149067A1 (en) 2012-06-14
RU2004122420A (ru) 2005-10-10
EP1572895A4 (en) 2007-07-25
RU2346049C2 (ru) 2009-02-10
DE60239495D1 (de) 2011-04-28
ATE502115T1 (de) 2011-04-15
US8574886B2 (en) 2013-11-05
CA2471148A1 (en) 2003-07-03
CA2803931A1 (en) 2003-07-03
DK1572895T3 (da) 2011-07-11
IL162302A (en) 2011-01-31
CN1636052A (zh) 2005-07-06
KR100885163B1 (ko) 2009-02-23
KR100879908B1 (ko) 2009-01-21
CA2471148C (en) 2013-04-09
US8093036B2 (en) 2012-01-10
US20140038235A1 (en) 2014-02-06
HUP0700154A2 (en) 2007-05-29
IL162302A0 (en) 2005-11-20
HU228693B1 (hu) 2013-05-28
US20030175902A1 (en) 2003-09-18
WO2003054163A2 (en) 2003-07-03
HU1300136D0 (hu) 2007-05-29
EP1572895B1 (en) 2011-03-16
US7811806B2 (en) 2010-10-12
US20110014662A1 (en) 2011-01-20

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