IL162302A0 - Methods for producing hyaluronan ina recombinant host cell - Google Patents

Methods for producing hyaluronan ina recombinant host cell

Info

Publication number
IL162302A0
IL162302A0 IL16230202A IL16230202A IL162302A0 IL 162302 A0 IL162302 A0 IL 162302A0 IL 16230202 A IL16230202 A IL 16230202A IL 16230202 A IL16230202 A IL 16230202A IL 162302 A0 IL162302 A0 IL 162302A0
Authority
IL
Israel
Prior art keywords
udp
glucose
gene
host cell
hyaluronan synthase
Prior art date
Application number
IL16230202A
Other languages
English (en)
Original Assignee
Novozymes Biopolymer As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biopolymer As filed Critical Novozymes Biopolymer As
Publication of IL162302A0 publication Critical patent/IL162302A0/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
IL16230202A 2001-12-21 2002-12-20 Methods for producing hyaluronan ina recombinant host cell IL162302A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34264401P 2001-12-21 2001-12-21
PCT/US2002/041067 WO2003054163A2 (en) 2001-12-21 2002-12-20 Methods for producing hyaluronan in a recombinant host cell

Publications (1)

Publication Number Publication Date
IL162302A0 true IL162302A0 (en) 2005-11-20

Family

ID=23342668

Family Applications (2)

Application Number Title Priority Date Filing Date
IL16230202A IL162302A0 (en) 2001-12-21 2002-12-20 Methods for producing hyaluronan ina recombinant host cell
IL162302A IL162302A (en) 2001-12-21 2004-06-02 Methods of producing hyaluronic acid in a bacillus host cell

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL162302A IL162302A (en) 2001-12-21 2004-06-02 Methods of producing hyaluronic acid in a bacillus host cell

Country Status (18)

Country Link
US (5) US7811806B2 (pt)
EP (1) EP1572895B1 (pt)
JP (1) JP2005525091A (pt)
KR (2) KR100879908B1 (pt)
CN (1) CN1636052B (pt)
AT (1) ATE502115T1 (pt)
AU (1) AU2002366711C1 (pt)
BR (1) BR0215220A (pt)
CA (2) CA2803931A1 (pt)
CZ (1) CZ2004765A3 (pt)
DE (1) DE60239495D1 (pt)
DK (1) DK1572895T3 (pt)
ES (1) ES2362354T3 (pt)
HU (2) HU228693B1 (pt)
IL (2) IL162302A0 (pt)
PL (2) PL212928B1 (pt)
RU (1) RU2346049C2 (pt)
WO (1) WO2003054163A2 (pt)

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CA2307842C (en) * 1997-10-31 2010-10-19 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase gene and uses thereof
JP2005514059A (ja) * 2001-06-13 2005-05-19 ザ ボード オブ リージェンツ オブ ザ ユニヴァーシティー オブ オクラホマ ヒアルロナンシンターゼ遺伝子およびそれらの発現
CZ2004765A3 (cs) * 2001-12-21 2004-12-15 Novozymes Biopolymer A/S Způsoby výroby hylauronanu v rekombinantní hostitelské buňce
AR040004A1 (es) * 2002-05-14 2005-03-09 Novozymes As Variantes de pectatoliasa
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DK1537229T3 (da) * 2002-07-26 2013-12-02 Novozymes Inc Fremgangsmåder til produktion af biologiske stoffer i pigmentmanglende mutanter af Bacillus-celler
EP3018140A3 (en) * 2004-01-09 2016-08-17 Basf Se Inactivation of glutamylpolypeptide synthesis in bacillus
CA2563173A1 (en) * 2004-03-31 2005-10-20 Novozymes Biopolymer A/S Methods for producing hyaluronic acid in a bacillus cell
US7002007B2 (en) * 2004-05-28 2006-02-21 Calcigen Corporation Production of high molecular weight hyaluronates
WO2005123915A1 (en) 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
EP1640457A1 (de) * 2004-09-23 2006-03-29 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan
WO2006032538A1 (en) 2004-09-23 2006-03-30 Bayer Cropscience Gmbh Methods and means for producing hyaluronan
EP1817347B1 (en) * 2004-11-24 2017-05-17 Albumedix A/S Method of cross-linking hyaluronic acid with divinylsulfone
KR20070106702A (ko) * 2004-12-30 2007-11-05 노보자임스 바이오폴리머 에이/에스 알파 히드록시산의 중합체와 연결된 히알루론산
EP1696032A1 (de) * 2005-02-23 2006-08-30 Bayer CropScience GmbH Verfahren und Mittel zur Herstellung von Hyaluronan in Pilzen
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
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EP1932909A4 (en) * 2005-08-25 2009-04-08 Toyo Boseki VEGETABLE PRODUCING HYALURONIC ACID
EP1772052A1 (de) 2005-10-05 2007-04-11 Bayer CropScience GmbH Verbesserte Verfahren und Mittel zur Herstellung von Hyaluronan
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US20080038780A1 (en) 2006-02-15 2008-02-14 Novozymes Biopolymer A/S Production of low molecular weight hyaluronic acid
CN101405303B (zh) * 2006-03-14 2011-11-23 诺维信生物制药丹麦公司 丙烯酸酯化的透明质酸
US8202986B2 (en) 2006-08-04 2012-06-19 Novozymes Biopolymer AIS Branched hyaluronic acid and method of manufacture
WO2008067423A2 (en) 2006-11-29 2008-06-05 Novozymes, Inc. Methods of improving the introduction of dna into bacterial cells
EP2104739B1 (en) 2006-12-21 2013-06-19 Novozymes Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells
EP2031053A1 (en) * 2007-08-31 2009-03-04 The University Of Queensland Production of HA
AU2008291695A1 (en) * 2007-08-31 2009-03-05 Sugar Industry Innovation Pty Ltd Production of hyaluronic acid
EP2036983A1 (de) 2007-09-12 2009-03-18 Bayer CropScience AG Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren
CN101878230B (zh) * 2007-12-19 2012-11-21 赢创高施米特有限公司 乳液中的交联透明质酸
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IT1401498B1 (it) 2010-07-30 2013-07-26 Mero Srl Idrogelo a base di acido ialuronico e suo uso in ortopedia
IT1402385B1 (it) 2010-09-09 2013-09-04 Fidia Farmaceutici Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis
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CN102911887A (zh) * 2011-08-02 2013-02-06 山东省生物药物研究院 一种利用毕赤酵母基因调控发酵生产不同分子量透明质酸的方法
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KR101736790B1 (ko) * 2012-02-21 2017-05-17 블루메이지 프레다 바이오팜 컴퍼니 리미티드 바실러스, 히알루론산 효소, 및 그의 용도
JP6306519B2 (ja) * 2012-02-22 2018-04-04 ノボザイムス アクティーゼルスカブ 高度発酵制御
WO2014100837A1 (en) 2012-12-17 2014-06-26 Bui The Duy Computer aided implantation of body implants
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CA2896038C (en) 2015-07-03 2022-08-09 Glycobiosciences Inc. Polymer matrix compositions comprising a high concentration of bio-fermented sodium hyaluronate and uses thereof
CN106755022B (zh) * 2015-11-25 2020-08-04 中国科学院大连化学物理研究所 乙酰葡萄糖胺磷酸变位酶AtAGM编码基因及其酶、制备、应用与酶活性检测方法
MX2019000133A (es) 2016-07-05 2019-04-22 Novozymes As Variantes de pectato liasa y polinucleotidos que las codifican.
CN107653280B (zh) * 2016-07-25 2021-05-04 安琪酵母股份有限公司 透明质酸发酵用的组合物、培养基和方法及其应用
CN106381279B (zh) * 2016-08-29 2019-11-05 中国药科大学 一种细菌胞外多糖、其制备方法及其应用
WO2020122430A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 비병원성 세균을 이용하여 히알루론산 생산을 위한 발현 시스템 및 상기 발현 시스템을 이용한 히알루론산 생산방법
WO2020122429A1 (ko) * 2018-12-10 2020-06-18 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
KR102135044B1 (ko) * 2018-12-10 2020-07-17 대화제약 주식회사 히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
CN110055201B (zh) * 2019-03-05 2021-05-28 江南大学 一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法
RU2719140C1 (ru) 2019-07-26 2020-04-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии
RU2723722C1 (ru) * 2019-07-26 2020-06-17 Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех" ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ
CN111518825B (zh) * 2020-04-30 2022-10-11 浙江工业大学 一种多基因组合表达制备蛹虫草多糖的方法
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Also Published As

Publication number Publication date
AU2002366711A1 (en) 2003-07-09
PL212928B1 (pl) 2012-12-31
AU2002366711B2 (en) 2007-11-29
BR0215220A (pt) 2007-01-09
PL399352A1 (pl) 2012-10-08
EP1572895A4 (en) 2007-07-25
KR100879908B1 (ko) 2009-01-21
US8137951B2 (en) 2012-03-20
ATE502115T1 (de) 2011-04-15
US20140038235A1 (en) 2014-02-06
CA2471148A1 (en) 2003-07-03
US20120149067A1 (en) 2012-06-14
HU1300136D0 (hu) 2007-05-29
CA2471148C (en) 2013-04-09
DE60239495D1 (de) 2011-04-28
US20030175902A1 (en) 2003-09-18
US8574886B2 (en) 2013-11-05
WO2003054163A3 (en) 2005-10-27
HUP0700154A2 (en) 2007-05-29
EP1572895B1 (en) 2011-03-16
CA2803931A1 (en) 2003-07-03
AU2002366711C1 (en) 2009-01-22
RU2004122420A (ru) 2005-10-10
US7811806B2 (en) 2010-10-12
HU228693B1 (hu) 2013-05-28
KR20040085142A (ko) 2004-10-07
US20110014662A1 (en) 2011-01-20
ES2362354T3 (es) 2011-07-04
US8093036B2 (en) 2012-01-10
JP2005525091A (ja) 2005-08-25
CN1636052B (zh) 2014-07-02
RU2346049C2 (ru) 2009-02-10
EP1572895A2 (en) 2005-09-14
PL375190A1 (en) 2005-11-28
DK1572895T3 (da) 2011-07-11
KR100885163B1 (ko) 2009-02-23
CZ2004765A3 (cs) 2004-12-15
WO2003054163A2 (en) 2003-07-03
CN1636052A (zh) 2005-07-06
IL162302A (en) 2011-01-31
KR20080018965A (ko) 2008-02-28
US20110189737A1 (en) 2011-08-04

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