WO1994000463A3 - Production of hyaluronic acid by transeformed microorganisms - Google Patents

Production of hyaluronic acid by transeformed microorganisms Download PDF

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Publication number
WO1994000463A3
WO1994000463A3 PCT/EP1993/001575 EP9301575W WO9400463A3 WO 1994000463 A3 WO1994000463 A3 WO 1994000463A3 EP 9301575 W EP9301575 W EP 9301575W WO 9400463 A3 WO9400463 A3 WO 9400463A3
Authority
WO
WIPO (PCT)
Prior art keywords
hyaluronic acid
acid synthase
cells
synthase
producing
Prior art date
Application number
PCT/EP1993/001575
Other languages
French (fr)
Other versions
WO1994000463A2 (en
Inventor
Peter Prehm
Michael O'regan
Lanfranco Callegaro
Sabine Lellig
Original Assignee
Murst Italian
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Murst Italian filed Critical Murst Italian
Publication of WO1994000463A2 publication Critical patent/WO1994000463A2/en
Publication of WO1994000463A3 publication Critical patent/WO1994000463A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

An isolated DNA fragment comprising a nucleotide sequence encoding hyaluronic acid synthase is provided. Also provided are recombinant expression vectors comprising this sequence, capable of expressing hyaluronic acid synthase in prokaryotic and eukaryotic cells, as well as host cells harboring such expression vectors. Such cells are capable of producing hyaluronic acid synthase, hyaluronic acid, or both, which can be recovered from these cells, the culture medium, or both. Also provided are a method of producing hyaluronic acid synthase, methods of producing hyaluronic acid both in vivo and in vitro, and hyaluronic acid produced by these methods. In the case of in vitro production, the molecular weight of hyaluronic acid can be varied by varying the time of incubation of hyaluronic acid precursors in the presence of hyaluronic acid synthase and/or the reaction conditions. Lastly, a method of purifying hyaluronic acid synthase from cultured cells is also provided.
PCT/EP1993/001575 1992-06-19 1993-06-21 Production of hyaluronic acid by transeformed microorganisms WO1994000463A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITPD92A000110 1992-06-19
ITPD920110A IT1260153B (en) 1992-06-19 1992-06-19 IDENTIFICATION OF A DNA FRAGMENT CODING THE HYALURONIC ACID SYNTHETASE (HAS) AND ITS NUCLEOTID SEQUENCE

Publications (2)

Publication Number Publication Date
WO1994000463A2 WO1994000463A2 (en) 1994-01-06
WO1994000463A3 true WO1994000463A3 (en) 1994-09-29

Family

ID=11389978

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1993/001575 WO1994000463A2 (en) 1992-06-19 1993-06-21 Production of hyaluronic acid by transeformed microorganisms

Country Status (2)

Country Link
IT (1) IT1260153B (en)
WO (1) WO1994000463A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7091008B1 (en) 1994-07-01 2006-08-15 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase genes and expression thereof in Bacillus hosts
US6951743B2 (en) 1997-10-31 2005-10-04 University Of Oklahoma Board Of Regents Hyaluronan synthase genes and expression thereof in bacillus hosts
US6455304B1 (en) 1994-07-01 2002-09-24 The Board Of Regents Of The University Of Oklahoma Hyaluronate synthase gene and uses thereof
US6423514B1 (en) * 1996-04-22 2002-07-23 Millennium Pharmaceuticals, Inc. Mammalian hyaluronan synthases, nucleic acids and uses thereof
CN101113436B (en) 1997-10-31 2013-02-06 俄克拉何马大学董事会 Hyaluronan synthase gene and uses thereof
DE69828193T2 (en) 1997-10-31 2005-12-01 The Board Of Regents Of The University Of Oklahoma, Norman HYALURONAN SYNTHASE GENE AND ITS USE
EP1073460A4 (en) * 1998-04-02 2001-05-23 Univ Oklahoma Nucleic acid encoding hyaluronan synthase and methods of use
US7223571B2 (en) 1998-04-02 2007-05-29 The Board Of Regents Of The Universtiy Of Oklahoma Targeted glycosaminoglycan polymers by polymer grafting and methods of making and using same
US6987023B2 (en) 1998-04-02 2006-01-17 The Board Of Regents Of The University Of Oklahoma DNA encoding hyaluronan synthase from Pasteurella multocida and methods of use
US20060188966A1 (en) 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
US7094581B2 (en) 1998-10-26 2006-08-22 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthases and methods of making and using same
KR101322227B1 (en) 2011-09-30 2013-10-28 일동제약주식회사 Streptococcus dysgalactiae ID9103 and method for production of hyaluronic acid using the same
WO2013116819A1 (en) * 2012-02-03 2013-08-08 University Of Rochester Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991003559A1 (en) * 1989-08-29 1991-03-21 Board Of Regents, The University Of Texas System Dna encoding hyaluronate synthase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991003559A1 (en) * 1989-08-29 1991-03-21 Board Of Regents, The University Of Texas System Dna encoding hyaluronate synthase

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A. MAUSOLF ET AL.: "Shedding of hyaluronate synthase from Streptococci", THE BIOCHEMICAL JOURNAL, vol. 267, no. 1, 1 April 1990 (1990-04-01), LONDON, UK, pages 191 - 196, XP009052569 *
M. LANSING ET AL.: "Hyaluronate synthase: cloning and sequencing of the gene from Streptococcus sp.", THE BIOCHEMICAL JOURNAL, vol. 289, no. 1, 1 January 1993 (1993-01-01), LONDON, UK, pages 179 - 184 *
P. PREHM AND A. MAUSOLF: "Isolation of streptococcal hyaluronate synthase", THE BIOCHEMICAL JOURNAL, vol. 235, no. 3, 1 May 1986 (1986-05-01), LONDON, UK, pages 887 - 889 *

Also Published As

Publication number Publication date
ITPD920110A1 (en) 1993-12-19
IT1260153B (en) 1996-03-28
WO1994000463A2 (en) 1994-01-06
ITPD920110A0 (en) 1992-06-19

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