DE60226592D1 - Desoxycytidinkinase-mutanten mit erweiterter enzymaktivität - Google Patents

Desoxycytidinkinase-mutanten mit erweiterter enzymaktivität

Info

Publication number
DE60226592D1
DE60226592D1 DE60226592T DE60226592T DE60226592D1 DE 60226592 D1 DE60226592 D1 DE 60226592D1 DE 60226592 T DE60226592 T DE 60226592T DE 60226592 T DE60226592 T DE 60226592T DE 60226592 D1 DE60226592 D1 DE 60226592D1
Authority
DE
Germany
Prior art keywords
protein
cells
vector
mutagen
evolving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60226592T
Other languages
English (en)
Inventor
Philippe Marliere
Sylvie Pochet
Madeleine Bouzon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut Pasteur de Lille
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut Pasteur de Lille filed Critical Centre National de la Recherche Scientifique CNRS
Application granted granted Critical
Publication of DE60226592D1 publication Critical patent/DE60226592D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Ecology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60226592T 2001-04-10 2002-04-10 Desoxycytidinkinase-mutanten mit erweiterter enzymaktivität Expired - Lifetime DE60226592D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0104856A FR2823219B1 (fr) 2001-04-10 2001-04-10 Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
PCT/FR2002/001252 WO2002083892A2 (fr) 2001-04-10 2002-04-10 Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie

Publications (1)

Publication Number Publication Date
DE60226592D1 true DE60226592D1 (de) 2008-06-26

Family

ID=8862148

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60226592T Expired - Lifetime DE60226592D1 (de) 2001-04-10 2002-04-10 Desoxycytidinkinase-mutanten mit erweiterter enzymaktivität

Country Status (12)

Country Link
US (2) US7547513B2 (de)
EP (2) EP1383890B1 (de)
JP (1) JP2004528844A (de)
AT (1) ATE395416T1 (de)
AU (1) AU2002307996B2 (de)
CA (1) CA2444026A1 (de)
DE (1) DE60226592D1 (de)
DK (1) DK1383890T3 (de)
ES (1) ES2306780T3 (de)
FR (1) FR2823219B1 (de)
PT (1) PT1383890E (de)
WO (1) WO2002083892A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA05008857A (es) * 2003-02-18 2006-03-09 Metabolic Explorer Sa Procedimiento de preparacion de microorganismos evolucionados que permite la creacion o la modificacion de vias metabolicas.
US7419811B2 (en) 2003-02-28 2008-09-02 The Board Of Trustees Of The University Of Illinois Use of specifically engineered enzymes to enhance the efficacy of prodrugs
FR2852968B1 (fr) 2003-03-28 2007-10-26 Pasteur Institut Procede de modification in vivo de l'activite de synthese d'un metabolite par modification d'un gene dont l'activite n'est pas l'activite originelle
US7892838B2 (en) 2003-03-28 2011-02-22 Institut Pasteur Method for the in vivo modification of the synthesis activity of a metabolite by means of the modification of a gene the activity of which is not the original activity
FR2868432B1 (fr) * 2004-03-30 2007-11-02 Pasteur Institut N-desoxyribosyltransferases de lactobacillus fermantum et application a la synthese enzymatique de 2',3'-didesoxynucleosides et de 2'3'-didehydro- 2'3'-didesoxynucleosides
EP1781788A2 (de) * 2004-06-30 2007-05-09 ZGene A/S Desoxycytidinkinase- und desoxyadenosinkinase-enzyme aus huhn und deren verwendung
DE102005037349A1 (de) * 2005-08-08 2007-02-15 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vivo
US8349318B2 (en) 2008-05-19 2013-01-08 The Board Of Trustees Of The University Of Illinois Use of specifically engineered enzymes to enhance the efficacy of prodrugs
JP6205642B2 (ja) * 2013-05-16 2017-10-04 国立大学法人 千葉大学 テルペン合成酵素をコードする遺伝子のスクリーニング方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030194807A1 (en) * 1992-11-02 2003-10-16 Roberto Crea Walk-through mutagenesis
AU691194B2 (en) * 1993-06-25 1998-05-14 Yale University Chemically modified oligonucleotide for site-directed mutagenesis
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
AU1923795A (en) * 1994-02-18 1995-09-04 University Of Washington Methods and compositions for screening for anti-aids drugs
US6063376A (en) 1995-01-13 2000-05-16 Human Genome Sciences, Inc. Human deoxycytidine kinase 2
US6015705A (en) 1995-07-24 2000-01-18 Rijksuniversiteit Groningen Method of modifying the DNA of an organism, DNA sequence, method of detection and isolation of a gene capable of modifying the DNA of an organism and organisms comprising modified DNA
EP0866796B1 (de) * 1995-09-22 2000-11-29 Amersham Pharmacia Biotech UK Limited Verstärkung der mutagenese von nukleinsäuren
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6790605B1 (en) * 1995-12-07 2004-09-14 Diversa Corporation Methods for obtaining a desired bioactivity or biomolecule using DNA libraries from an environmental source
US6764835B2 (en) * 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
WO1997029196A1 (fr) 1996-02-09 1997-08-14 Rhone-Poulenc Rorer S.A. Variants de la thymidine kinase, sequences d'acides nucleiques correspondantes et leur utilisation en therapie genique
EP0906420A1 (de) * 1996-06-07 1999-04-07 Massachusetts Institute Of Technology Programmiertem regelmässige mutagenese
JP3172710B2 (ja) * 1997-07-07 2001-06-04 理化学研究所 Ｒｎａポリメラーゼ
GB9716231D0 (en) * 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US20020151019A1 (en) * 1999-01-19 2002-10-17 John Shanklin Mutant fatty acid desaturase and methods for directed mutagenesis
US20030049614A1 (en) * 1999-10-06 2003-03-13 Holly Hurlbut Hogrefe Compositions for dna amplification, synthesis, and mutagenesis
CN1429268A (zh) * 2000-05-12 2003-07-09 沃尔夫冈·肯特 新型脱氧核苷激酶多底物变体
JP3859947B2 (ja) * 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
FR2813314B1 (fr) * 2000-08-25 2004-05-07 Biomethodes Procede de mutagenese dirigee massive
GB0106567D0 (en) * 2001-03-16 2001-05-09 Arrow Therapeutics Ltd Mutuagenesis Technique
US7647184B2 (en) * 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
EP1451295B1 (de) * 2001-11-02 2015-06-03 Athersys, Inc. Verfahren zur mutation von genen in zellen und tieren mit insertionsmutagenese
US20030171543A1 (en) * 2002-03-05 2003-09-11 Bott Richard R. High throughput mutagenesis screening method
CA2382470A1 (en) * 2002-04-29 2003-10-29 Institut Pasteur Mutant ndp kinases for antiviral nucleotide analog activation and therapeutic uses thereof

Also Published As

Publication number Publication date
US7547513B2 (en) 2009-06-16
EP1383890A2 (de) 2004-01-28
FR2823219A1 (fr) 2002-10-11
PT1383890E (pt) 2008-08-25
FR2823219B1 (fr) 2003-07-04
EP1383890B1 (de) 2008-05-14
JP2004528844A (ja) 2004-09-24
WO2002083892A3 (fr) 2003-12-04
EP1921143A3 (de) 2009-06-03
EP1921143A2 (de) 2008-05-14
US20070037269A1 (en) 2007-02-15
CA2444026A1 (fr) 2002-10-24
WO2002083892A2 (fr) 2002-10-24
AU2002307996B2 (en) 2007-05-24
ES2306780T3 (es) 2008-11-16
US20100136693A1 (en) 2010-06-03
ATE395416T1 (de) 2008-05-15
DK1383890T3 (da) 2008-09-15

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