ATE439432T1 - Verfahren zur herstellung von säugetier-trypsinen - Google Patents

Verfahren zur herstellung von säugetier-trypsinen

Info

Publication number
ATE439432T1
ATE439432T1 AT03755759T AT03755759T ATE439432T1 AT E439432 T1 ATE439432 T1 AT E439432T1 AT 03755759 T AT03755759 T AT 03755759T AT 03755759 T AT03755759 T AT 03755759T AT E439432 T1 ATE439432 T1 AT E439432T1
Authority
AT
Austria
Prior art keywords
mammalian trypsin
nucleic acid
encoding
fusarium venenatum
fusarium
Prior art date
Application number
AT03755759T
Other languages
English (en)
Inventor
Randy Berka
Kimberly Brown
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE439432T1 publication Critical patent/ATE439432T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)
AT03755759T 2002-08-30 2003-08-29 Verfahren zur herstellung von säugetier-trypsinen ATE439432T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40717002P 2002-08-30 2002-08-30
PCT/US2003/027074 WO2004020612A1 (en) 2002-08-30 2003-08-29 Methods for producing mammalian trypsins

Publications (1)

Publication Number Publication Date
ATE439432T1 true ATE439432T1 (de) 2009-08-15

Family

ID=31978430

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03755759T ATE439432T1 (de) 2002-08-30 2003-08-29 Verfahren zur herstellung von säugetier-trypsinen

Country Status (8)

Country Link
US (1) US20040043455A1 (de)
EP (1) EP1546302B1 (de)
CN (1) CN1694953B (de)
AT (1) ATE439432T1 (de)
AU (1) AU2003273256A1 (de)
DE (1) DE60328802D1 (de)
DK (1) DK1546302T3 (de)
WO (1) WO2004020612A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011047900A2 (en) 2009-10-22 2011-04-28 Glaxosmithkline Biologicals S.A. Novel process
WO2011138229A1 (en) 2010-05-03 2011-11-10 Glaxosmithkline Biologicals S.A. Novel method
GB201011502D0 (en) 2010-07-08 2010-08-25 Glaxosmithkline Biolog Sa Novel process
BE1022132B1 (fr) 2013-08-30 2016-02-19 Glaxosmithkline Biologicals S.A. Nouvelle methode.
CN106459937A (zh) * 2014-05-27 2017-02-22 诺维信公司 用于产生脂肪酶的方法
CN107090427A (zh) * 2017-06-23 2017-08-25 友康恒业生物科技（北京）有限公司 一种基因重组胰蛋白酶细胞消化液
CN113430187A (zh) * 2021-06-23 2021-09-24 山东黎宁科技新材料有限公司 一种皮革软化用复合酶及其制备方法与应用
CN115975996A (zh) * 2022-11-24 2023-04-18 中国科学院华南植物园 FpOPSB基因和蛋白在预防香蕉冠腐病中的应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5288627A (en) 1988-01-07 1994-02-22 Novo Nordisk A/S Endoprotease from Fusarium oxysporumDSM 2672 for use in detergents
DK52393D0 (de) 1993-05-05 1993-05-05 Novo Nordisk As
CN101659926A (zh) * 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
WO1997000316A1 (en) 1995-06-16 1997-01-03 Novo Nordisk A/S A process for producing trypsin (trypsinogen)
US5945328A (en) 1995-06-16 1999-08-31 Novo Nordisk A/S Process for producing trypsin (trypsinogen)
US5948746A (en) 1995-07-19 1999-09-07 Novo Nordisk A/S Proteolytic enzymes
AU1750697A (en) 1996-01-19 1997-08-11 Novo Nordisk Biotech, Inc. Morphological mutants of filamentous fungi
CN1226283A (zh) 1996-06-28 1999-08-18 诺沃挪第克公司 具有葡聚糖酶活性的重组酶
AU9260598A (en) 1997-08-22 1999-03-16 Boehringer Mannheim Gmbh Zymogenic protease precursors that can be autocatalytically activated and their use
EP1078081B1 (de) 1998-05-20 2008-05-14 Novozymes, Inc. Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
WO2000017332A1 (en) 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
DE69932345T2 (de) 1998-10-26 2007-07-19 Novozymes A/S Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
AU2612000A (en) * 1999-01-13 2000-08-01 Novozymes Biotech, Inc. Methods for producing polypeptides in cyclohexadepsipeptide-deficient cells
US6361973B1 (en) 1999-03-22 2002-03-26 Novozymes Biotech, Inc. Promoters for expressing genes in a fungal cell
WO2001055429A2 (en) 2000-01-24 2001-08-02 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
DE50204952D1 (de) 2001-02-01 2005-12-22 Roche Diagnostics Gmbh Verfahren zur herstellung von rekombinantem trypsin

Also Published As

Publication number Publication date
WO2004020612A1 (en) 2004-03-11
EP1546302A1 (de) 2005-06-29
EP1546302B1 (de) 2009-08-12
CN1694953A (zh) 2005-11-09
AU2003273256A1 (en) 2004-03-19
CN1694953B (zh) 2010-05-12
DE60328802D1 (de) 2009-09-24
US20040043455A1 (en) 2004-03-04
EP1546302A4 (de) 2006-08-16
DK1546302T3 (da) 2009-12-14

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