DE60328802D1 - Verfahren zur herstellung von säugetier-trypsinen - Google Patents

Verfahren zur herstellung von säugetier-trypsinen

Info

Publication number
DE60328802D1
DE60328802D1 DE60328802T DE60328802T DE60328802D1 DE 60328802 D1 DE60328802 D1 DE 60328802D1 DE 60328802 T DE60328802 T DE 60328802T DE 60328802 T DE60328802 T DE 60328802T DE 60328802 D1 DE60328802 D1 DE 60328802D1
Authority
DE
Germany
Prior art keywords
mammalian trypsin
nucleic acid
encoding
fusarium venenatum
fusarium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60328802T
Other languages
English (en)
Inventor
Randy Berka
Kimberly Brown
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DE60328802D1 publication Critical patent/DE60328802D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE60328802T 2002-08-30 2003-08-29 Verfahren zur herstellung von säugetier-trypsinen Expired - Lifetime DE60328802D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40717002P 2002-08-30 2002-08-30
PCT/US2003/027074 WO2004020612A1 (en) 2002-08-30 2003-08-29 Methods for producing mammalian trypsins

Publications (1)

Publication Number Publication Date
DE60328802D1 true DE60328802D1 (de) 2009-09-24

Family

ID=31978430

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60328802T Expired - Lifetime DE60328802D1 (de) 2002-08-30 2003-08-29 Verfahren zur herstellung von säugetier-trypsinen

Country Status (8)

Country Link
US (1) US20040043455A1 (de)
EP (1) EP1546302B1 (de)
CN (1) CN1694953B (de)
AT (1) ATE439432T1 (de)
AU (1) AU2003273256A1 (de)
DE (1) DE60328802D1 (de)
DK (1) DK1546302T3 (de)
WO (1) WO2004020612A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011047900A2 (en) 2009-10-22 2011-04-28 Glaxosmithkline Biologicals S.A. Novel process
WO2011138229A1 (en) 2010-05-03 2011-11-10 Glaxosmithkline Biologicals S.A. Novel method
GB201011502D0 (en) 2010-07-08 2010-08-25 Glaxosmithkline Biolog Sa Novel process
JP6851827B2 (ja) 2013-08-30 2021-03-31 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 細胞培養中でのウイルスの大規模製造
US10683489B2 (en) 2014-05-27 2020-06-16 Novozymes A/S Methods for producing lipases
CN107090427A (zh) * 2017-06-23 2017-08-25 友康恒业生物科技（北京）有限公司 一种基因重组胰蛋白酶细胞消化液
CN113430187A (zh) * 2021-06-23 2021-09-24 山东黎宁科技新材料有限公司 一种皮革软化用复合酶及其制备方法与应用
CN115975996A (zh) * 2022-11-24 2023-04-18 中国科学院华南植物园 FpOPSB基因和蛋白在预防香蕉冠腐病中的应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5288627A (en) 1988-01-07 1994-02-22 Novo Nordisk A/S Endoprotease from Fusarium oxysporumDSM 2672 for use in detergents
DK52393D0 (de) 1993-05-05 1993-05-05 Novo Nordisk As
EP0777737B1 (de) 1994-06-30 2005-05-04 Novozymes Biotech, Inc. Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US5945328A (en) 1995-06-16 1999-08-31 Novo Nordisk A/S Process for producing trypsin (trypsinogen)
JPH11507207A (ja) 1995-06-16 1999-06-29 ノボ ノルディスク アクティーゼルスカブ トリプシン（トリプシノーゲン）の製造法
US5948746A (en) 1995-07-19 1999-09-07 Novo Nordisk A/S Proteolytic enzymes
EP0877801B1 (de) 1996-01-19 2005-04-27 Novozymes Biotech, Inc. Morphologische mutanten von filamentösen pilzen
EP0910627A1 (de) 1996-06-28 1999-04-28 Novo Nordisk A/S Rekombinantes enzym mit dextranase aktivität
WO1999010503A1 (de) 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
CN1231579C (zh) 1998-05-20 2005-12-14 诺沃奇梅兹生物技术有限公司 trichodiene合成酶及其制备方法
EP1141263A4 (de) 1998-09-21 2002-08-28 Lilly Co Eli Herstellung von löslichen rekombinanten trypsinanalogen
DK1124949T3 (da) 1998-10-26 2006-11-06 Novozymes As Konstruktion og screening af et DNA-bibliotek af interesse i trådformede svampeceller
JP4860820B2 (ja) * 1999-01-13 2012-01-25 ノボザイムス，インコーポレイティド シクロヘキサデプシペプチド欠損細胞におけるポリペプチドの産生方法
US6361973B1 (en) 1999-03-22 2002-03-26 Novozymes Biotech, Inc. Promoters for expressing genes in a fungal cell
WO2001055429A2 (en) 2000-01-24 2001-08-02 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
CN1545553B (zh) 2001-02-01 2011-09-21 弗·哈夫曼-拉罗切有限公司 生产重组胰蛋白酶的方法

Also Published As

Publication number Publication date
US20040043455A1 (en) 2004-03-04
CN1694953A (zh) 2005-11-09
EP1546302B1 (de) 2009-08-12
WO2004020612A1 (en) 2004-03-11
EP1546302A4 (de) 2006-08-16
EP1546302A1 (de) 2005-06-29
ATE439432T1 (de) 2009-08-15
DK1546302T3 (da) 2009-12-14
CN1694953B (zh) 2010-05-12
AU2003273256A1 (en) 2004-03-19

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