ATE532870T1 - Verfahren zur herstellung von transglutaminase - Google Patents

Verfahren zur herstellung von transglutaminase

Info

Publication number
ATE532870T1
ATE532870T1 AT00962986T AT00962986T ATE532870T1 AT E532870 T1 ATE532870 T1 AT E532870T1 AT 00962986 T AT00962986 T AT 00962986T AT 00962986 T AT00962986 T AT 00962986T AT E532870 T1 ATE532870 T1 AT E532870T1
Authority
AT
Austria
Prior art keywords
transglutaminase
coryneform bacterium
foreign protein
pro
secretory production
Prior art date
Application number
AT00962986T
Other languages
English (en)
Inventor
Yoshimi Kikuchi
Masayo Date
Yukiko Umezawa
Keiichi Yokoyama
Hiroshi Matsui
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Application granted granted Critical
Publication of ATE532870T1 publication Critical patent/ATE532870T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT00962986T 1999-09-30 2000-09-29 Verfahren zur herstellung von transglutaminase ATE532870T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP28009899 1999-09-30
JP2000194043 2000-06-28
PCT/JP2000/006780 WO2001023591A1 (en) 1999-09-30 2000-09-29 Process for producing transglutaminase

Publications (1)

Publication Number Publication Date
ATE532870T1 true ATE532870T1 (de) 2011-11-15

Family

ID=26553616

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00962986T ATE532870T1 (de) 1999-09-30 2000-09-29 Verfahren zur herstellung von transglutaminase

Country Status (12)

Country Link
US (2) US7723067B2 (de)
EP (1) EP1219713B1 (de)
JP (1) JP4320769B2 (de)
KR (1) KR100565030B1 (de)
CN (1) CN100497632C (de)
AT (1) ATE532870T1 (de)
AU (1) AU7449400A (de)
BR (1) BR0014059B1 (de)
CA (1) CA2391961C (de)
DK (1) DK1219713T3 (de)
ES (1) ES2372806T3 (de)
WO (1) WO2001023591A1 (de)

Families Citing this family (43)

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BR0113304A (pt) * 2000-08-17 2004-01-06 Ajinomoto Kk Método para planejar e preparar uma transglutaminase mutante, mtg mutante transglutaminase mutante, gene, dna recombinante, microorganismos, método para produzir uma mtg mutante ou uma transglutaminase mutante, e, cristais de mtg monoclìnicos
EP1375664B1 (de) 2001-03-30 2009-11-04 Ajinomoto Co., Inc. Verfahren zur sekretion und produktion von protein
US7101695B2 (en) 2002-03-01 2006-09-05 Szu-Yi Chou Method of producing transglutaminase having broad substrate activity
US7485438B2 (en) 2002-03-01 2009-02-03 Szu-Yi Chou Method of producing polyvalent antigens
JP4323147B2 (ja) * 2002-09-10 2009-09-02 天野エンザイム株式会社 トランスグルタミナーゼ生産菌
AU2003268693A1 (en) * 2002-09-30 2004-04-19 Ajinomoto Co., Inc. Method of producing stable isotope-labeled protein
ES2356149T3 (es) 2003-03-07 2011-04-05 Ajinomoto Co., Inc. Procedimiento para producir transglutaminasa microbiana.
DK1748077T3 (da) 2004-04-20 2013-04-08 Ajinomoto Kk Fremgangsmåde til fremstilling af protein
EP1920050A1 (de) * 2005-08-18 2008-05-14 Novo Nordisk Health Care AG Transglutaminase-substrate mit erhöhter spezifität
WO2008020074A1 (en) * 2006-08-18 2008-02-21 Novo Nordisk Health Care Ag Transglutaminase variants with improved specificity
CN101631860B (zh) 2007-02-15 2012-07-18 味之素株式会社 引入二硫键的转谷氨酰胺酶
EP2118132A1 (de) * 2007-02-22 2009-11-18 Novo Nordisk Health Care AG Transglutaminasevarianten mit verbesserter spezifität
JP5093228B2 (ja) 2007-03-29 2012-12-12 味の素株式会社 接着用酵素製剤及び接着成形食品の製造方法
JPWO2009066785A1 (ja) 2007-11-19 2011-04-07 味の素株式会社 繊維加工物及びその製造法
CN101945995B (zh) 2008-02-13 2013-06-12 天野酶株式会社 稳定型转谷氨酰胺酶及其制造法
TWI444146B (zh) 2008-09-25 2014-07-11 Ajinomoto Kk 黏著成型食品用酵素製劑及黏著成型食品之製造方法
CN102341497B (zh) * 2009-03-06 2013-11-06 味之素株式会社 放线菌来源的耐热性转谷氨酰胺酶
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
JP6064912B2 (ja) 2011-10-25 2017-01-25 味の素株式会社 タンパク質の分泌生産法
IN2014CN04039A (de) 2011-11-02 2015-10-23 Ajinomoto Kk
CN103946372B (zh) 2011-11-02 2016-06-08 味之素株式会社 用于分泌产生蛋白质的方法
CN102586167B (zh) * 2012-03-01 2013-07-24 华南理工大学 一株重组的枯草芽孢杆菌及由其生产转谷氨酰胺酶的方法
CN103060283B (zh) * 2012-12-28 2014-05-28 天津科技大学 一种抑制枯草芽孢杆菌谷氨酰胺转胺酶活性的多肽及其筛选方法和应用
WO2014126260A1 (ja) * 2013-02-18 2014-08-21 味の素株式会社 タンパク質の沈殿による製造方法
CN103146661A (zh) * 2013-03-07 2013-06-12 哈尔滨工业大学 MgCl2胁迫提高茂原链霉菌谷氨酰胺转氨酶产量的方法
RU2013119826A (ru) 2013-04-29 2014-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") Коринеформная бактерия и способ получения гетерологичных гибридных белков
EP3287523B1 (de) 2015-04-24 2022-06-29 Ajinomoto Co., Inc. Verfahren zur sekretorischen herstellung eines proteins
PL3533875T3 (pl) * 2016-09-01 2023-06-12 Ningxia Eppen Biotech Co. Ltd Corynebacterium do wytwarzania L-lizyny przez fermentację
AU2017347016B2 (en) 2016-10-21 2021-09-23 Ajinomoto Co., Inc. Secretory production method for protein
CN109937258B (zh) 2016-10-21 2023-06-23 味之素株式会社 蛋白质的分泌产生方法
WO2018179834A1 (en) 2017-03-28 2018-10-04 Ajinomoto Co., Inc. Method for producing rna
WO2019078095A1 (en) 2017-10-16 2019-04-25 Ajinomoto Co., Inc. PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY
CN107574159B (zh) * 2017-10-26 2020-05-08 江南大学 一种以活性形式表达的谷氨酰胺转氨酶的突变体
BR112020016770A2 (pt) 2018-02-20 2020-12-15 Ajinomoto Co., Inc. Método para induzir silenciamento de rna e para produzir uma composição para induzir silenciamento de rna em um organismo-alvo, e, composição para induzir silenciamento de rna em um organismo-alvo
EP3783106A4 (de) 2018-04-20 2022-01-05 Ajinomoto Co., Inc. Verfahren zur sekretorischen herstellung eines proteins
CN115298306A (zh) * 2020-03-13 2022-11-04 居里公司 转谷氨酰胺酶变体
CN113528479B (zh) * 2020-04-13 2023-01-24 中国科学院微生物研究所 一种转谷氨酰胺酶的高效制备方法及其专用工程菌
WO2021251383A1 (ja) 2020-06-11 2021-12-16 味の素株式会社 タンパク質の製造法
CN111944778B (zh) * 2020-08-14 2022-06-21 安徽医学高等专科学校 谷氨酰胺转胺酶突变体及其编码基因和应用
WO2023282315A1 (ja) 2021-07-07 2023-01-12 味の素株式会社 非天然アミノ酸含有タンパク質の分泌生産法
EP4509613A1 (de) 2022-04-15 2025-02-19 Ajinomoto Co., Inc. Verfahren zur herstellung von kultiviertem fleisch
EP4526437A1 (de) * 2022-05-20 2025-03-26 Curie Co. Inc. Propeptidvarianten zur modulation der aktivität von transglutaminasen
WO2024172163A1 (ja) 2023-02-16 2024-08-22 味の素株式会社 タンパク質の分泌生産法

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JPH0736754B2 (ja) 1993-07-16 1995-04-26 三共株式会社 ヒト・膵臓エラスターゼｉｉｉｂ
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JP3711658B2 (ja) * 1996-10-07 2005-11-02 味の素株式会社 コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質
JP3305609B2 (ja) 1997-03-06 2002-07-24 ダイハツ工業株式会社 車両用スペアタイヤの支持構造
JPH10244947A (ja) 1997-03-06 1998-09-14 Toyoda Gosei Co Ltd ステアリングホイール芯金

Also Published As

Publication number Publication date
CN100497632C (zh) 2009-06-10
ES2372806T3 (es) 2012-01-26
DK1219713T3 (da) 2011-12-12
WO2001023591A1 (en) 2001-04-05
US7972829B2 (en) 2011-07-05
US7723067B2 (en) 2010-05-25
US20100297729A1 (en) 2010-11-25
EP1219713B1 (de) 2011-11-09
US20030082746A1 (en) 2003-05-01
KR20020038787A (ko) 2002-05-23
AU7449400A (en) 2001-04-30
JP4320769B2 (ja) 2009-08-26
EP1219713A4 (de) 2005-02-02
BR0014059B1 (pt) 2013-02-05
CN1377413A (zh) 2002-10-30
CA2391961A1 (en) 2001-04-05
EP1219713A1 (de) 2002-07-03
BR0014059A (pt) 2002-05-14
KR100565030B1 (ko) 2006-03-30
CA2391961C (en) 2008-12-02

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