BR0014059A - Processos para produzir uma proteìna secretora heteróloga, e para a produção secretora de uma transglutaminase madura, peptidase especìfica para prolina, polipeptìdeo, e, molécula de ácido nucleico - Google Patents
Processos para produzir uma proteìna secretora heteróloga, e para a produção secretora de uma transglutaminase madura, peptidase especìfica para prolina, polipeptìdeo, e, molécula de ácido nucleicoInfo
- Publication number
- BR0014059A BR0014059A BR0014059-7A BR0014059A BR0014059A BR 0014059 A BR0014059 A BR 0014059A BR 0014059 A BR0014059 A BR 0014059A BR 0014059 A BR0014059 A BR 0014059A
- Authority
- BR
- Brazil
- Prior art keywords
- transglutaminase
- coryneform bacterium
- foreign protein
- secretory
- secretory production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/104—Aminoacyltransferases (2.3.2)
- C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/02—Aminoacyltransferases (2.3.2)
- C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"PROCESSOS PARA PRODUZIR UMA PROTEìNA SECRETORA HETERóLOGA, E PARA A PRODUçãO SECRETORA DE UMA TRANSGLUTAMINASE MADURA, PEPTIDASE ESPECìFICA PARA PROLINA, POLIPEPTìDEO, E, MOLéCULA DE áCIDO NUCLEICO". A presente invenção refere-se a um processo para a produção secretora de uma proteína estranha, em particular transglutaminase, por meio de uma bactéria coryneform. De acordo com a presente invenção, proporciona-se um processo para a produção secretora de uma proteína estranha, em particular transglutaminase, fazendo-se com que uma bactéria coryneform produza uma proteína estranha industrialmente útil, em particular transglutaminase, e libere eficientemente o produto extracelularmente (isto é, produção secretora). Uma proteína estranha desejada, em particular transglutaminase, é produzida utilizando-se uma construção de expressão em que a seq³ência de genes da proteína estranha desejada contendo a parte pró-estrutura, em particular seq³ência de genes de pró-transglutaminase, é ligada a jusante de uma seq³ência codificando a região de peptídeo sinal de uma bactéria coryneform introduzindo esta construção genética expressional numa bactéria coryneform, cultivando a assim transformada bactéria coryneform, e tratando a proteína liberada extracelularmente com uma protease, etc. para clivar e eliminar a parte pró.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28009899 | 1999-09-30 | ||
| JP2000194043 | 2000-06-28 | ||
| PCT/JP2000/006780 WO2001023591A1 (fr) | 1999-09-30 | 2000-09-29 | Procédé de production de transglutaminase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR0014059A true BR0014059A (pt) | 2002-05-14 |
| BR0014059B1 BR0014059B1 (pt) | 2013-02-05 |
Family
ID=26553616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0014059-7A BR0014059B1 (pt) | 1999-09-30 | 2000-09-29 | processo para produzir transglutaminase. |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7723067B2 (pt) |
| EP (1) | EP1219713B1 (pt) |
| JP (1) | JP4320769B2 (pt) |
| KR (1) | KR100565030B1 (pt) |
| CN (1) | CN100497632C (pt) |
| AT (1) | ATE532870T1 (pt) |
| AU (1) | AU7449400A (pt) |
| BR (1) | BR0014059B1 (pt) |
| CA (1) | CA2391961C (pt) |
| DK (1) | DK1219713T3 (pt) |
| ES (1) | ES2372806T3 (pt) |
| WO (1) | WO2001023591A1 (pt) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2260051C2 (ru) * | 2000-08-17 | 2005-09-10 | Адзиномото Ко., Инк. | Способ модифицирования трансглутаминаз микроорганизмов |
| EP1375664B1 (en) | 2001-03-30 | 2009-11-04 | Ajinomoto Co., Inc. | Method for the secretion and production of protein |
| US7485438B2 (en) | 2002-03-01 | 2009-02-03 | Szu-Yi Chou | Method of producing polyvalent antigens |
| US7101695B2 (en) | 2002-03-01 | 2006-09-05 | Szu-Yi Chou | Method of producing transglutaminase having broad substrate activity |
| JP4323147B2 (ja) | 2002-09-10 | 2009-09-02 | 天野エンザイム株式会社 | トランスグルタミナーゼ生産菌 |
| EP1548116A4 (en) * | 2002-09-30 | 2006-04-05 | Ajinomoto Kk | PROCESS FOR PRODUCING A PROTEIN MARKED BY A STABLE ISOTOPE |
| CN100381569C (zh) | 2003-03-07 | 2008-04-16 | 味之素株式会社 | 微生物转谷氨酰胺酶的生产方法 |
| JP4730302B2 (ja) * | 2004-04-20 | 2011-07-20 | 味の素株式会社 | タンパク質の製造法 |
| CN101287757A (zh) * | 2005-08-18 | 2008-10-15 | 诺沃-诺迪斯克保健股份有限公司 | 特异性改进的转谷氨酰胺酶变异体 |
| US20090318349A1 (en) * | 2006-08-18 | 2009-12-24 | Novo Nordisk Health Care Ag | Transglutaminase variants with improved specificity |
| KR101162049B1 (ko) | 2007-02-15 | 2012-07-04 | 아지노모토 가부시키가이샤 | 디설파이드 결합 도입 트랜스글루타미나제 |
| JP2010518842A (ja) * | 2007-02-22 | 2010-06-03 | ノボ ノルディスク ヘルス ケア アーゲー | 改善された特異性を有するトランスグルタミナーゼ変異体 |
| PL2138049T3 (pl) | 2007-03-29 | 2014-11-28 | Ajinomoto Kk | Preparat enzymatyczny do adhezji i sposób wytwarzania uformowanego adhezyjnie produktu żywnościowego |
| CN101868575A (zh) | 2007-11-19 | 2010-10-20 | 味之素株式会社 | 纤维加工物及其制造方法 |
| ES2616020T3 (es) | 2008-02-13 | 2017-06-09 | Amano Enzyme Inc. | Transglutaminasa estabilizada y procedimiento para la producción de la misma |
| TWI444146B (zh) | 2008-09-25 | 2014-07-11 | Ajinomoto Kk | 黏著成型食品用酵素製劑及黏著成型食品之製造方法 |
| CN102341497B (zh) * | 2009-03-06 | 2013-11-06 | 味之素株式会社 | 放线菌来源的耐热性转谷氨酰胺酶 |
| US20100316764A1 (en) * | 2009-06-10 | 2010-12-16 | Engrain, LLC | Flour supplement compositions and methods for preparing wheat flour |
| EP2772546B1 (en) * | 2011-10-25 | 2019-05-15 | Ajinomoto Co., Inc. | Secretion production method for protein |
| WO2013065772A1 (ja) | 2011-11-02 | 2013-05-10 | 味の素株式会社 | タンパク質の分泌生産法 |
| IN2014CN04039A (pt) | 2011-11-02 | 2015-10-23 | Ajinomoto Kk | |
| CN102586167B (zh) * | 2012-03-01 | 2013-07-24 | 华南理工大学 | 一株重组的枯草芽孢杆菌及由其生产转谷氨酰胺酶的方法 |
| CN103060283B (zh) * | 2012-12-28 | 2014-05-28 | 天津科技大学 | 一种抑制枯草芽孢杆菌谷氨酰胺转胺酶活性的多肽及其筛选方法和应用 |
| WO2014126260A1 (ja) | 2013-02-18 | 2014-08-21 | 味の素株式会社 | タンパク質の沈殿による製造方法 |
| CN103146661A (zh) * | 2013-03-07 | 2013-06-12 | 哈尔滨工业大学 | MgCl2胁迫提高茂原链霉菌谷氨酰胺转氨酶产量的方法 |
| RU2013119826A (ru) | 2013-04-29 | 2014-11-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Коринеформная бактерия и способ получения гетерологичных гибридных белков |
| WO2016171224A1 (ja) | 2015-04-24 | 2016-10-27 | 味の素株式会社 | タンパク質の分泌生産法 |
| US11242545B2 (en) | 2016-09-01 | 2022-02-08 | Ningxia Eppen Biotech Co., Ltd | Corynebacterium for producing L-lysine by fermentation |
| JP7159867B2 (ja) | 2016-10-21 | 2022-10-25 | 味の素株式会社 | タンパク質の分泌生産法 |
| EP3530748B1 (en) | 2016-10-21 | 2021-06-02 | Ajinomoto Co., Inc. | Method for the secretory production proteins |
| JP2020512007A (ja) | 2017-03-28 | 2020-04-23 | 味の素株式会社 | Ｒｎａの製造方法 |
| WO2019078095A1 (en) | 2017-10-16 | 2019-04-25 | Ajinomoto Co., Inc. | PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY |
| CN107574159B (zh) * | 2017-10-26 | 2020-05-08 | 江南大学 | 一种以活性形式表达的谷氨酰胺转氨酶的突变体 |
| BR112020016770A2 (pt) | 2018-02-20 | 2020-12-15 | Ajinomoto Co., Inc. | Método para induzir silenciamento de rna e para produzir uma composição para induzir silenciamento de rna em um organismo-alvo, e, composição para induzir silenciamento de rna em um organismo-alvo |
| KR20210002575A (ko) | 2018-04-20 | 2021-01-08 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산법 |
| CN113528479B (zh) * | 2020-04-13 | 2023-01-24 | 中国科学院微生物研究所 | 一种转谷氨酰胺酶的高效制备方法及其专用工程菌 |
| AU2021289499A1 (en) | 2020-06-11 | 2023-02-02 | Ajinomoto Co., Inc. | Method for producing protein |
| CN111944778B (zh) * | 2020-08-14 | 2022-06-21 | 安徽医学高等专科学校 | 谷氨酰胺转胺酶突变体及其编码基因和应用 |
| CN117642511A (zh) | 2021-07-07 | 2024-03-01 | 味之素株式会社 | 含非天然氨基酸的蛋白质的分泌生产方法 |
| WO2023225666A1 (en) * | 2022-05-20 | 2023-11-23 | Curie Co. Inc. | Pro-peptide variants for modulating activity of transglutaminases |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8800352A1 (es) * | 1985-04-05 | 1987-10-16 | Sankyo Co | Un procedimiento para producir un compuesto capaz de funcionar como una elastasa pancreatica humana |
| RU2091490C1 (ru) | 1985-10-25 | 1997-09-27 | Зимодженетикс, Инк. | Способ получения гетерологичного полипептида в эукариотических микроорганизмов |
| US4965194A (en) * | 1986-08-21 | 1990-10-23 | Toyo Boseki Kabushiki Kaisha | Pyruvate oxidase and an analytical method using the same |
| WO1988009821A1 (en) * | 1987-06-12 | 1988-12-15 | Massachusetts Institute Of Technology | Coryneform expression and secretion system |
| JPH05244947A (ja) * | 1992-03-04 | 1993-09-24 | Mercian Corp | 新規ポストプロリンエンドペプチダーゼおよびその製造方法 |
| JPH0736754B2 (ja) | 1993-07-16 | 1995-04-26 | 三共株式会社 | ヒト・膵臓エラスターゼｉｉｉｂ |
| JPH09316095A (ja) * | 1996-05-27 | 1997-12-09 | Mitsubishi Chem Corp | 新規シグナルペプチド |
| JP3711658B2 (ja) * | 1996-10-07 | 2005-11-02 | 味の素株式会社 | コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質 |
| JP3305609B2 (ja) | 1997-03-06 | 2002-07-24 | ダイハツ工業株式会社 | 車両用スペアタイヤの支持構造 |
| JPH10244947A (ja) | 1997-03-06 | 1998-09-14 | Toyoda Gosei Co Ltd | ステアリングホイール芯金 |
-
2000
- 2000-09-29 CA CA002391961A patent/CA2391961C/en not_active Expired - Lifetime
- 2000-09-29 EP EP00962986A patent/EP1219713B1/en not_active Expired - Lifetime
- 2000-09-29 WO PCT/JP2000/006780 patent/WO2001023591A1/ja not_active Application Discontinuation
- 2000-09-29 KR KR1020027004159A patent/KR100565030B1/ko active IP Right Grant
- 2000-09-29 AT AT00962986T patent/ATE532870T1/de active
- 2000-09-29 CN CNB008137331A patent/CN100497632C/zh not_active Expired - Lifetime
- 2000-09-29 BR BRPI0014059-7A patent/BR0014059B1/pt active IP Right Grant
- 2000-09-29 DK DK00962986.6T patent/DK1219713T3/da active
- 2000-09-29 AU AU74494/00A patent/AU7449400A/en not_active Abandoned
- 2000-09-29 JP JP2001526973A patent/JP4320769B2/ja not_active Expired - Lifetime
- 2000-09-29 ES ES00962986T patent/ES2372806T3/es not_active Expired - Lifetime
-
2002
- 2002-04-01 US US10/112,488 patent/US7723067B2/en not_active Expired - Fee Related
-
2010
- 2010-02-26 US US12/714,151 patent/US7972829B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| KR20020038787A (ko) | 2002-05-23 |
| US20030082746A1 (en) | 2003-05-01 |
| BR0014059B1 (pt) | 2013-02-05 |
| US7723067B2 (en) | 2010-05-25 |
| EP1219713A1 (en) | 2002-07-03 |
| EP1219713A4 (en) | 2005-02-02 |
| US7972829B2 (en) | 2011-07-05 |
| ES2372806T3 (es) | 2012-01-26 |
| JP4320769B2 (ja) | 2009-08-26 |
| WO2001023591A1 (fr) | 2001-04-05 |
| CN1377413A (zh) | 2002-10-30 |
| CN100497632C (zh) | 2009-06-10 |
| DK1219713T3 (da) | 2011-12-12 |
| US20100297729A1 (en) | 2010-11-25 |
| CA2391961C (en) | 2008-12-02 |
| CA2391961A1 (en) | 2001-04-05 |
| AU7449400A (en) | 2001-04-30 |
| EP1219713B1 (en) | 2011-11-09 |
| KR100565030B1 (ko) | 2006-03-30 |
| ATE532870T1 (de) | 2011-11-15 |
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