ATE438722T1 - Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein - Google Patents

Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein

Info

Publication number
ATE438722T1
ATE438722T1 AT01930179T AT01930179T ATE438722T1 AT E438722 T1 ATE438722 T1 AT E438722T1 AT 01930179 T AT01930179 T AT 01930179T AT 01930179 T AT01930179 T AT 01930179T AT E438722 T1 ATE438722 T1 AT E438722T1
Authority
AT
Austria
Prior art keywords
sugar chain
reduction
protein
mannose phosphate
glycoprotein
Prior art date
Application number
AT01930179T
Other languages
English (en)
Inventor
Masami Miura
Masaaki Hirose
Taeko Miwa
Hiroyuki Irie
Shinobu Kuwae
Kenmi Miyano
Wataru Otani
Hideyuki Ohi
Original Assignee
Mitsubishi Tanabe Pharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Tanabe Pharma Corp filed Critical Mitsubishi Tanabe Pharma Corp
Application granted granted Critical
Publication of ATE438722T1 publication Critical patent/ATE438722T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT01930179T 2000-05-17 2001-05-16 Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein ATE438722T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000144643 2000-05-17
PCT/JP2001/004070 WO2001088143A1 (fr) 2000-05-17 2001-05-16 Procede d'elaboration de proteine avec reduction de chaine saccharose acide, et glycoproteine resultante

Publications (1)

Publication Number Publication Date
ATE438722T1 true ATE438722T1 (de) 2009-08-15

Family

ID=18651270

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01930179T ATE438722T1 (de) 2000-05-17 2001-05-16 Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein

Country Status (9)

Country Link
US (3) US7198921B2 (de)
EP (1) EP1283265B1 (de)
JP (2) JP4969011B2 (de)
AT (1) ATE438722T1 (de)
AU (1) AU2001256762A1 (de)
DE (1) DE60139467D1 (de)
DK (1) DK1283265T3 (de)
ES (1) ES2328106T3 (de)
WO (1) WO2001088143A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001256762A1 (en) * 2000-05-17 2001-11-26 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
CN100385006C (zh) 2001-01-19 2008-04-30 陶氏化学公司 利用植物细胞分泌性生产具有人型糖链的糖蛋白的方法
JP4861830B2 (ja) * 2003-12-24 2012-01-25 グライコフィ， インコーポレイテッド 糖タンパク質の産生において、グリカンのマンノシルリン酸化を除去する方法
WO2005106010A2 (en) * 2004-04-29 2005-11-10 Glycofi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
EP1934605B1 (de) * 2005-09-22 2014-03-26 Prosci Incorporated In hefemutanten produzierte glykosylierte polypeptide und verwendungsverfahren dafür
CA2679732A1 (en) * 2007-03-07 2008-09-18 Glycofi, Inc. Production of glycoproteins with modified fucosylation
US8026083B2 (en) 2007-04-03 2011-09-27 Oxyrane Uk Limited Yarrowia lipolytica and Pichia pastoris HAC1 nucleic acids
CA2684370C (en) 2007-04-17 2017-10-17 Plant Research International B.V. Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
CN101903531B (zh) * 2007-12-19 2013-09-18 格利科菲公司 用于蛋白质生产的酵母菌株
EP2245151B1 (de) * 2008-02-20 2014-12-24 GlycoFi, Inc. Vektoren und hefestämme für die proteinproduktion
CA2715212A1 (en) * 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
MX2011001706A (es) 2008-08-12 2011-03-24 Glycofi Inc Vectores mejorados y cepas de levadura para produccion de proteina: sobreexpresion de ca2+ atpasa.
CN102333872B (zh) 2009-02-25 2014-07-09 默沙东公司 在糖工程化的酵母巴斯德毕赤酵母中对半乳糖同化途径的代谢工程化
CA2775938C (en) 2009-09-29 2021-08-10 Oxyrane Uk Limited Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
CN102648286A (zh) 2009-10-16 2012-08-22 默沙东公司 在巴斯德毕赤氏酵母中生产缺乏与针对宿主细胞抗原的抗体的可检测交叉结合活性的蛋白质的方法
CN102725396A (zh) 2009-10-30 2012-10-10 默沙东公司 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
CA2777487A1 (en) 2009-10-30 2011-05-05 Merck Sharp & Dohme Corp. Methods for the production of recombinant proteins with improved secretion efficiencies
JP2013511272A (ja) 2009-11-19 2013-04-04 オキシレイン ユーケー リミテッド 哺乳類様複合ｎ−グリカンを生成する酵母株
US8715963B2 (en) 2010-02-24 2014-05-06 Merck Sharp & Dohme Corp. Method for increasing N-glycosylation site occupancy on therapeutic glycoproteins produced in Pichia pastoris
WO2011149999A2 (en) 2010-05-27 2011-12-01 Merck Sharp & Dohme Corp. Method for preparing antibodies having improved properties
EP2622088A2 (de) 2010-09-29 2013-08-07 Oxyrane UK Limited Mannosidasen zum entkappen von mannose-1-phospho-6-mannose-bindungen und demannosylierung von phosphorylierten n-glykanen sowie verfahren zur ermöglichung der zellulären aufnahme von glykoproteinen durch säugerzellen
CN103261432A (zh) 2010-09-29 2013-08-21 奥克西雷恩英国有限公司 磷酸化的n-聚糖的脱甘露糖基化
CN107459577A (zh) 2010-12-01 2017-12-12 默沙东公司 表面锚定的fc‑诱饵抗体展示系统
JP2014518608A (ja) 2011-02-25 2014-08-07 メルク・シャープ・アンド・ドーム・コーポレーション 修飾ｏ−グリコシル化を有するタンパク質の製造のための酵母株
CA2834589A1 (en) 2011-05-25 2012-11-29 Merck Sharp & Dohme Corp. Method for preparing fc-containing polypeptides having improved properties
CA2853341A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
KR20190114003A (ko) 2012-03-15 2019-10-08 옥시레인 유케이 리미티드 폼페병의 치료를 위한 방법 및 물질
EP2847368B1 (de) 2012-05-11 2020-08-19 Merck Sharp & Dohme Corp. Oberflächenverankertes leichtketten-köder-antikörperdisplaysystem
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
CN104736694B (zh) 2012-10-22 2020-08-11 默沙东公司 Crz1突变型真菌细胞
CA2890048C (en) 2012-12-03 2022-05-03 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
JPH093097A (ja) 1995-06-16 1997-01-07 Green Cross Corp:The ピキア属酵母由来の糖鎖伸長タンパク、該蛋白質をコードするｄｎａ、修飾該ｄｎａおよび形質転換体
JP2770010B2 (ja) 1996-03-29 1998-06-25 工業技術院長 酵母のマンノース−１−リン酸転移を正に制御する遺伝子ならびにこの遺伝子の欠損変異株を利用する高マンノース型中性糖鎖の製造方法
US6747137B1 (en) * 1998-02-13 2004-06-08 Genome Therapeutics Corporation Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics
AU2001256762A1 (en) * 2000-05-17 2001-11-26 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
WO2002000879A2 (en) * 2000-06-28 2002-01-03 Glycofi, Inc. Methods for producing modified glycoproteins

Also Published As

Publication number Publication date
EP1283265B1 (de) 2009-08-05
JP2012105652A (ja) 2012-06-07
US7872111B2 (en) 2011-01-18
US20090004725A1 (en) 2009-01-01
ES2328106T3 (es) 2009-11-10
US7488591B2 (en) 2009-02-10
DE60139467D1 (de) 2009-09-17
EP1283265A4 (de) 2004-10-20
AU2001256762A1 (en) 2001-11-26
US20070202569A1 (en) 2007-08-30
US20040014170A1 (en) 2004-01-22
EP1283265A1 (de) 2003-02-12
US7198921B2 (en) 2007-04-03
JP4969011B2 (ja) 2012-07-04
WO2001088143A1 (fr) 2001-11-22
DK1283265T3 (da) 2009-11-09

Similar Documents

Publication Publication Date Title
ATE438722T1 (de) Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein
DE60228961D1 (de) Verfahren zur herstellung von l-aminosäuren durch enterobacteriaceae-stämmen mit verstärkter expression des mop-b
ATE392430T1 (de) Verfahren zur herstellung von dipeptiden
ATE474847T1 (de) Verfahren zur herstellung von dipeptiden
ATE414162T1 (de) Verfahren und materialien zur herstellung von glucosamin
DE69941991D1 (de) Verfahren zur Herstellung von L-Aminosäure
EP1239041A3 (de) Verfahren zur Produktion von L-Aminosäuren mittels Bakterien der Gattung Escherichia
ATE426021T1 (de) Verfahren zur herstellung von biopterinen
DE10390418D2 (de) Verfahren zur Herstellung von rekombinanten Proteinen in Mikroorganismen
ATE312809T1 (de) Verfahren zur herstellung von gabapentin
ATE341640T1 (de) Verfahren zur herstellung von nicht-proteinogenen l-aminosäuren
DE60211772D1 (de) Verfahren zur Herstellung von Proteine
ATE458037T1 (de) Grosstechnisch geeigneter mikroorganismus
DE60038281D1 (de) Verfahren zur herstellung von optisch aktiven pyridinethanol-derivaten
DE59807664D1 (de) Verfahren zur herstellung von 3-cyano-2,4-dihalogen-5-fluor-benzoesäuren
ATE386027T1 (de) Verfahren zur herstellung von valsartan
DE50109809D1 (de) Verfahren zur Herstellung von D- oder L-Menthol
ATE341558T1 (de) Verfahren zur herstellung von topiramate
DE602005016857D1 (de) Verfahren zur Herstellung von Tripeptid-Zwischenprodukten zur Herstellung von LHRH-Antagonisten
WO2003040291A3 (de) Gene die für kohlenstoffmetabolismus- und energieproduktion-proteine codieren
ATE386015T1 (de) Verfahren zur herstellung von alkyl-n-(3- dimethylamino)alkylcarbamaten
ATE399209T1 (de) Verfahren zur herstellung von coenzym q 10
WO2003040289A3 (de) Gene die für dna-replikations- und pathogenese proteine codieren
DE69930118D1 (de) Verfahren zur herstellung von proteinen in transformierten hefezellen
DE50203529D1 (de) Verfahren zur herstellung von desclarithromycin, sowie zwischenprodukte

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties