DE60139467D1 - Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein - Google Patents

Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein

Info

Publication number
DE60139467D1
DE60139467D1 DE60139467T DE60139467T DE60139467D1 DE 60139467 D1 DE60139467 D1 DE 60139467D1 DE 60139467 T DE60139467 T DE 60139467T DE 60139467 T DE60139467 T DE 60139467T DE 60139467 D1 DE60139467 D1 DE 60139467D1
Authority
DE
Germany
Prior art keywords
sugar chain
reduction
glycoprotein
protein
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60139467T
Other languages
English (en)
Inventor
Masami Miura
Masaaki Hirose
Taeko Miwa
Hiroyuki Irie
Shinobu Kuwae
Kenmi Miyano
Wataru Otani
Hideyuki Ohi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Mitsubishi Tanabe Pharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Tanabe Pharma Corp filed Critical Mitsubishi Tanabe Pharma Corp
Application granted granted Critical
Publication of DE60139467D1 publication Critical patent/DE60139467D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
DE60139467T 2000-05-17 2001-05-16 Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein Expired - Lifetime DE60139467D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000144643 2000-05-17
PCT/JP2001/004070 WO2001088143A1 (fr) 2000-05-17 2001-05-16 Procede d'elaboration de proteine avec reduction de chaine saccharose acide, et glycoproteine resultante

Publications (1)

Publication Number Publication Date
DE60139467D1 true DE60139467D1 (de) 2009-09-17

Family

ID=18651270

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60139467T Expired - Lifetime DE60139467D1 (de) 2000-05-17 2001-05-16 Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein

Country Status (9)

Country Link
US (3) US7198921B2 (de)
EP (1) EP1283265B1 (de)
JP (2) JP4969011B2 (de)
AT (1) ATE438722T1 (de)
AU (1) AU2001256762A1 (de)
DE (1) DE60139467D1 (de)
DK (1) DK1283265T3 (de)
ES (1) ES2328106T3 (de)
WO (1) WO2001088143A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001256762A1 (en) * 2000-05-17 2001-11-26 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
CA2434364C (en) 2001-01-19 2013-03-19 The Dow Chemical Company Method for secretory production of glycoprotein having human-type sugar chain using plant cell
ES2528739T3 (es) * 2003-12-24 2015-02-12 Glycofi, Inc. Métodos para eliminar la manosilfosforilación de glucanos en la producción de glucoproteínas
CA2565125A1 (en) 2004-04-29 2005-11-10 Glycofi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
CN101313216A (zh) * 2005-09-22 2008-11-26 普洛茨股份有限公司 酵母突变体中产生的糖基化多肽及其使用方法
AU2008227024A1 (en) * 2007-03-07 2008-09-18 Glycofi, Inc. Production of glycoproteins with modified fucosylation
KR101812400B1 (ko) * 2007-04-03 2017-12-27 옥시레인 유케이 리미티드 분자의 글리코실화
MX341988B (es) 2007-04-17 2016-09-08 Stichting Dienst Landbouwkunding Onderzoek Glicosilacion tipo mamifera en plantas para la expresion de glicosil-transferasas no mamiferas.
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
EP2235198B1 (de) * 2007-12-19 2018-08-29 GlycoFi, Inc. Hefestämme für die Proteinproduktion
CA2714166A1 (en) 2008-02-20 2009-08-27 Glycofi, Inc. Vectors and yeast strains for protein production
JP5731827B2 (ja) 2008-03-03 2015-06-10 グライコフィ， インコーポレイテッド 下等真核生物中での組換えタンパク質の表面ディスプレイ
US8067339B2 (en) 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
US8507224B2 (en) 2008-08-12 2013-08-13 Glycofi, Inc. Vectors and yeast strains for protein production: Ca2+ ATPase overexpression
CA2751730A1 (en) 2009-02-25 2010-09-02 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
US9598682B2 (en) 2009-09-29 2017-03-21 Vib Vzw Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
US9139632B2 (en) 2009-10-16 2015-09-22 Merck Sharp & Dohme Corp. Method for producing proteins in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens
EP2494030A4 (de) 2009-10-30 2013-03-27 Merck Sharp & Dohme Verfahren zur herstellung therapeutischer proteine in pichia pastoris ohne dipeptidyl-aminopeptidase-aktivität
EP2494053A4 (de) 2009-10-30 2013-10-30 Merck Sharp & Dohme Verfahren zur herstellung rekombinanter proteine mit erhöhter sekretionseffizienz
JP2013511272A (ja) 2009-11-19 2013-04-04 オキシレイン ユーケー リミテッド 哺乳類様複合ｎ−グリカンを生成する酵母株
JP5976549B2 (ja) 2010-02-24 2016-08-24 メルク・シャープ・エンド・ドーム・コーポレイション ピチア・パストリスにおいて産生される治療用糖タンパク質上のｎ−グリコシル化部位占拠を増加させるための方法
RU2604811C2 (ru) 2010-05-27 2016-12-10 Мерк Шарп Энд Домэ Корп. Способ получения антител с улучшенными свойствами
JP6151183B2 (ja) 2010-09-29 2017-06-21 オキシレイン ユーケー リミテッド マンノース−１−ホスホ−６−マンノース結合からキャップを外すことおよびリン酸化ｎ−グリカンを脱マンノシル化することができるマンノシダーゼならびに哺乳動物細胞による糖タンパク質の取り込みを促進する方法
WO2012042387A2 (en) 2010-09-29 2012-04-05 Oxyrane Uk Limited De-mannosylation of phosphorylated n-glycans
CN110042066A (zh) 2010-12-01 2019-07-23 默沙东公司 表面锚定的fc-诱饵抗体展示系统
CN103764837A (zh) 2011-02-25 2014-04-30 默沙东公司 用于生产具有修饰的o-糖基化的蛋白的酵母菌株
EP2714732A4 (de) 2011-05-25 2014-12-10 Merck Sharp & Dohme Verfahren zur herstellung fc-haltiger polypeptide mit verbesserten eigenschaften
AU2012329091A1 (en) 2011-10-28 2014-05-01 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
KR20190114003A (ko) 2012-03-15 2019-10-08 옥시레인 유케이 리미티드 폼페병의 치료를 위한 방법 및 물질
EP2847368B1 (de) 2012-05-11 2020-08-19 Merck Sharp & Dohme Corp. Oberflächenverankertes leichtketten-köder-antikörperdisplaysystem
WO2013174927A1 (en) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production of fucosylated glycoproteins
US20150275222A1 (en) 2012-10-22 2015-10-01 Bo Jiang Crz1 mutant fungal cells
WO2014088836A1 (en) 2012-12-03 2014-06-12 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
JPH093097A (ja) * 1995-06-16 1997-01-07 Green Cross Corp:The ピキア属酵母由来の糖鎖伸長タンパク、該蛋白質をコードするｄｎａ、修飾該ｄｎａおよび形質転換体
JP2770010B2 (ja) * 1996-03-29 1998-06-25 工業技術院長 酵母のマンノース−１−リン酸転移を正に制御する遺伝子ならびにこの遺伝子の欠損変異株を利用する高マンノース型中性糖鎖の製造方法
US6747137B1 (en) * 1998-02-13 2004-06-08 Genome Therapeutics Corporation Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics
AU2001256762A1 (en) 2000-05-17 2001-11-26 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
PT1522590E (pt) * 2000-06-28 2009-10-26 Glycofi Inc Métodos para a produção de glicoproteínas modificadas

Also Published As

Publication number Publication date
AU2001256762A1 (en) 2001-11-26
US7872111B2 (en) 2011-01-18
US20070202569A1 (en) 2007-08-30
WO2001088143A1 (fr) 2001-11-22
EP1283265A4 (de) 2004-10-20
ES2328106T3 (es) 2009-11-10
EP1283265B1 (de) 2009-08-05
DK1283265T3 (da) 2009-11-09
JP2012105652A (ja) 2012-06-07
US20090004725A1 (en) 2009-01-01
US20040014170A1 (en) 2004-01-22
US7198921B2 (en) 2007-04-03
EP1283265A1 (de) 2003-02-12
ATE438722T1 (de) 2009-08-15
US7488591B2 (en) 2009-02-10
JP4969011B2 (ja) 2012-07-04

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