AU9260598A - Zymogenic protease precursors that can be autocatalytically activated and their use - Google Patents

Zymogenic protease precursors that can be autocatalytically activated and their use

Info

Publication number
AU9260598A
AU9260598A AU92605/98A AU9260598A AU9260598A AU 9260598 A AU9260598 A AU 9260598A AU 92605/98 A AU92605/98 A AU 92605/98A AU 9260598 A AU9260598 A AU 9260598A AU 9260598 A AU9260598 A AU 9260598A
Authority
AU
Australia
Prior art keywords
protease
zymogenic
precursor
host cell
active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU92605/98A
Inventor
Wolfram Bode
Karl-Peter Hopfner
Robert Huber
Erhard Kopetzki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of AU9260598A publication Critical patent/AU9260598A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A process for the recombinant production of a protease is characterised by (a) transformation of a host cell with a recombinant nucleic acid which codes for a zymogenic protease precursor containing an autocatalytic cleavage site which does not occur naturally, this cleavage site being recognised by the active form of said protease, cleaving the zymogenic precursor into the active protease; (b) cultivation of the host cell such that the zymogenic protease precursor is created in the host cell in the form of inclusion bodies; (c) isolation and naturalisation of the inclusion bodies in such conditions that the protease part of the zymogenic precursor is produced with its natural conformation; and (d) autocatalytic cleavage of the naturalised zymogenic precursor to produce the active protease. This process is suitable to produce recombinant proteases in a simple manner and with high yields.
AU92605/98A 1997-08-22 1998-08-12 Zymogenic protease precursors that can be autocatalytically activated and their use Abandoned AU9260598A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP97114513 1997-08-22
EP97114513 1997-08-22
EP97117816 1997-10-15
EP97117816 1997-10-15
PCT/EP1998/005094 WO1999010503A1 (en) 1997-08-22 1998-08-12 Zymogenic protease precursors that can be autocatalytically activated and their use

Publications (1)

Publication Number Publication Date
AU9260598A true AU9260598A (en) 1999-03-16

Family

ID=26145739

Family Applications (1)

Application Number Title Priority Date Filing Date
AU92605/98A Abandoned AU9260598A (en) 1997-08-22 1998-08-12 Zymogenic protease precursors that can be autocatalytically activated and their use

Country Status (9)

Country Link
EP (1) EP1012307B1 (en)
JP (1) JP3947653B2 (en)
AT (1) ATE294869T1 (en)
AU (1) AU9260598A (en)
CA (1) CA2301698A1 (en)
DE (1) DE59812768D1 (en)
DK (1) DK1012307T3 (en)
IL (1) IL134653A0 (en)
WO (1) WO1999010503A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6159722A (en) 1997-12-03 2000-12-12 Boehringer Mannheim Gmbh Chimeric serine proteases
GB9911689D0 (en) * 1999-05-19 1999-07-21 Medical Res Council Refolding method
JP4820512B2 (en) * 1999-08-09 2011-11-24 サンド・アクチエンゲゼルシヤフト Protein production
CN1230542C (en) 1999-08-09 2005-12-07 山德士股份公司 Production of proteins by autoproteolytic cleavage
AU7053500A (en) * 1999-09-15 2001-04-17 Eli Lilly And Company Chymotrypsin-free trypsin
WO2001066771A2 (en) * 2000-03-03 2001-09-13 Zymogenetics, Inc. Human serine protease
CN1545553B (en) 2001-02-01 2011-09-21 弗·哈夫曼-拉罗切有限公司 Method for producing recombinant trypsin
DE60328802D1 (en) 2002-08-30 2009-09-24 Novozymes Inc PROCESS FOR THE PREPARATION OF MAMMALS TRYPSINS
EP1558731A4 (en) * 2002-10-04 2007-01-10 Schering Ag Modified hepsin molecules having a substitute activation sequence and uses thereof
RU2006100035A (en) * 2003-06-11 2006-08-27 Шеринг Акциенгезельшафт (De) NEW MODIFIED CORIN MOLECULES HAVING SUBSTITUTED ACTIVATING SEQUENCES, AND THEIR APPLICATION
JP2015092852A (en) * 2013-11-12 2015-05-18 倉敷紡績株式会社 Composition for biological tissue dispersion
WO2018174231A1 (en) * 2017-03-24 2018-09-27 株式会社カネカ Method for producing protease in yeast

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340740C (en) * 1987-12-08 1999-09-14 Eileen R. Mulvihill Co-expression in eukaryotic cells
DE4140699A1 (en) * 1991-01-11 1992-07-16 Boehringer Mannheim Gmbh RECOMBINANT IGA PROTEASE
CA2102673A1 (en) * 1992-11-13 1994-05-14 Michael G. Greaney Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith
AU4114596A (en) * 1994-12-09 1996-06-26 Novo Nordisk A/S A process of producing extracellular proteins in bacteria
AU6121796A (en) * 1995-06-16 1997-01-15 Novo Nordisk A/S A process for producing trypsin (trypsinogen)

Also Published As

Publication number Publication date
DK1012307T3 (en) 2005-09-05
DE59812768D1 (en) 2005-06-09
IL134653A0 (en) 2001-04-30
JP3947653B2 (en) 2007-07-25
WO1999010503A1 (en) 1999-03-04
JP2001514003A (en) 2001-09-11
CA2301698A1 (en) 1999-03-04
ATE294869T1 (en) 2005-05-15
EP1012307B1 (en) 2005-05-04
EP1012307A1 (en) 2000-06-28

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase