WO2000046375A3 - Methods for producing polypeptides in fungal cells - Google Patents

Methods for producing polypeptides in fungal cells Download PDF

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Publication number
WO2000046375A3
WO2000046375A3 PCT/US2000/002864 US0002864W WO0046375A3 WO 2000046375 A3 WO2000046375 A3 WO 2000046375A3 US 0002864 W US0002864 W US 0002864W WO 0046375 A3 WO0046375 A3 WO 0046375A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
methods
fungal cells
producing polypeptides
nucleic acid
Prior art date
Application number
PCT/US2000/002864
Other languages
French (fr)
Other versions
WO2000046375A2 (en
Inventor
Debbie S Yaver
Original Assignee
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc filed Critical Novo Nordisk Biotech Inc
Priority to EP00905971A priority Critical patent/EP1151106A2/en
Priority to AU27553/00A priority patent/AU2755300A/en
Priority to JP2000597435A priority patent/JP2002535990A/en
Publication of WO2000046375A2 publication Critical patent/WO2000046375A2/en
Publication of WO2000046375A3 publication Critical patent/WO2000046375A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent fungal cell under conditions conducive for the production of the polypeptide, wherein the mutant cell comprises a first nucleic acid sequence comprising a modification of one or more genes of a pacC pH signal transduction pathway or homologues thereof, and a second nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.
PCT/US2000/002864 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells WO2000046375A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00905971A EP1151106A2 (en) 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells
AU27553/00A AU2755300A (en) 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells
JP2000597435A JP2002535990A (en) 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24195599A 1999-02-02 1999-02-02
US09/241,955 1999-02-02

Publications (2)

Publication Number Publication Date
WO2000046375A2 WO2000046375A2 (en) 2000-08-10
WO2000046375A3 true WO2000046375A3 (en) 2000-11-23

Family

ID=22912879

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/002864 WO2000046375A2 (en) 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells

Country Status (5)

Country Link
EP (1) EP1151106A2 (en)
JP (1) JP2002535990A (en)
CN (1) CN1268746C (en)
AU (1) AU2755300A (en)
WO (1) WO2000046375A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164574B1 (en) * 1999-12-10 2003-05-16 Consejo Superior Investigacion IMPROVEMENT OF PENICILLIN PRODUCTION THROUGH THE USE OF MERODIPLOID TRANSGENIC BREEDS FOR A REGULATORY GENE.
EP2102366A4 (en) * 2006-12-10 2010-01-27 Dyadic International Inc Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
US20130084608A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
RU2645252C2 (en) 2012-01-05 2018-02-19 Глюкос Финланд Ой Felamentous fungi cell with protease deficit and methods of its application
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
CA2916905A1 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
KR101508353B1 (en) * 2013-08-30 2015-04-06 대상 주식회사 Mutants of Trichoderma reesei Producing Cellulase with Improved Activity and Their Uses
FR3018522B1 (en) * 2014-03-17 2018-02-02 IFP Energies Nouvelles MUTANT STRAINS OF TRICHODERMA REESEI
CN108064266A (en) 2014-07-21 2018-05-22 格利科斯芬兰公司 The preparation of the glycoprotein with mammal sample N- glycan in filamentous fungi
JP6473339B2 (en) * 2015-02-05 2019-02-20 本田技研工業株式会社 Neisseria gonorrhoeae mutants and transformants
KR101751966B1 (en) 2015-06-23 2017-06-30 대상 주식회사 Method for Producing Cellulase with Improved Activity by Mixed Culture of the Genus Trichoderma
CN114786473B (en) * 2019-10-10 2023-12-19 嘉士伯有限公司 Method for producing mutant plants

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998011203A1 (en) * 1996-09-13 1998-03-19 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
WO1999061651A2 (en) * 1998-05-27 1999-12-02 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide by modifying the copy number of a gene

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998011203A1 (en) * 1996-09-13 1998-03-19 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
WO1999061651A2 (en) * 1998-05-27 1999-12-02 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide by modifying the copy number of a gene

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
ARST JR H N ET AL.: "Two new genes involved in signalling ambient pH in Aspergillus nidulans.", MOLECULAR AND GENERAL GENETICS, vol. 245, no. 6, December 1994 (1994-12-01), pages 787 - 790, XP002144939 *
DENISON S H ET AL.: "Putative membrane components of signal transduction pathways for ambient pH regulation in Aspergillus and meiosis in Saccharomyces are homologous.", MOLECULAR MICROBIOLOGY, vol. 30, no. 2, October 1998 (1998-10-01), pages 259 - 264, XP000930049 *
DENISON S H ET AL.: "Signaling of ambient pH in Aspergillus involves a cysteine protease", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 48, 1 December 1995 (1995-12-01), pages 28519 - 28522, XP002144938 *
DENISON S H: "pH regulation of gene expression in fungi", FUNGAL GENETICS AND BIOLOGY, vol. 29, no. 2, March 2000 (2000-03-01), pages 61 - 71, XP000911751 *
FUTAI E ET AL.: "The protease activity of a calpain-like cysteine protease in Saccharomyces cerevisiae is required for alkaline adaptation and sporulation", MOLECULAR AND GENERAL GENETICS, vol. 260, no. 6, January 1999 (1999-01-01), pages 559 - 568, XP002144933 *
LAMBERT M ET AL.: "Genetic analysis of regulatory mutants affecting synthesis of extracellular proteinases in the yeast Yarrowia lipolytica: identification of a RIM101/pacC homolog", MOLECULAR AND CELLULAR BIOLOGY, vol. 17, no. 7, July 1997 (1997-07-01), pages 3966 - 3976, XP002144934 *
MACCABE A P ET AL.: "Opposite patterns of expression of two Aspergillus nidulans xylananse genes with respect to ambient pH", JOURNAL OF BACTERIOLOGY, vol. 180, no. 5, March 1998 (1998-03-01), pages 1331 - 1333, XP002144935 *
MACCHERONI JR W ET AL.: "The sequence of palF, an environmental pH response gene in Aspergillus nidulans", GENE, vol. 194, no. 2, 31 July 1997 (1997-07-31), pages 163 - 167, XP004086062 *
NEGRETE-URTASUN S ET AL.: "Ambient pH signal transduction in Aspergillus: completion of gene characterization", MOLECULAR MICROBIOLOGY, vol. 33, no. 5, July 1999 (1999-07-01), pages 994 - 1003, XP000938403 *
NEGRETE-URTASUN S ET AL.: "Characterization of the pH signal transduction pathway gene palA of Aspergillus nidulans and identification of possible homologs", JOURNAL OF BACTERIOLOGY, vol. 179, no. 5, March 1997 (1997-03-01), pages 1832 - 1835, XP002144937 *
VAN DEN HOMBERGH J P T W ET AL.: "Regulation of acid phosphatases in an Aspergillus niger pacC disruption strain", MOLECULAR AND GENERAL GENETICS, vol. 251, no. 5, 19 July 1996 (1996-07-19), pages 542 - 550, XP002144936 *
VAN DEN HOMBERGH J P T W ET AL: "Aspergillus as a host for heterologous protein production: the problem of proteases", TRENDS IN BIOTECHNOLOGY, vol. 15, no. 7, 1 July 1997 (1997-07-01), pages 256 - 263, XP004092682 *
YAVER D S ET AL.: "Using DNA-tagged mutagenesis to improve heterologous protein production in Aspergillus oryzae.", FUNGAL GENETICS AND BIOLOGY, vol. 29, no. 1, February 2000 (2000-02-01), pages 28 - 37, XP000925375 *

Also Published As

Publication number Publication date
WO2000046375A2 (en) 2000-08-10
CN1268746C (en) 2006-08-09
EP1151106A2 (en) 2001-11-07
JP2002535990A (en) 2002-10-29
AU2755300A (en) 2000-08-25
CN1342204A (en) 2002-03-27

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