WO2000032757A3 - Plant vitamin e biosynthetic enzymes - Google Patents

Plant vitamin e biosynthetic enzymes Download PDF

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Publication number
WO2000032757A3
WO2000032757A3 PCT/US1999/028588 US9928588W WO0032757A3 WO 2000032757 A3 WO2000032757 A3 WO 2000032757A3 US 9928588 W US9928588 W US 9928588W WO 0032757 A3 WO0032757 A3 WO 0032757A3
Authority
WO
WIPO (PCT)
Prior art keywords
vitamin
biosynthetic enzyme
biosynthetic enzymes
plant vitamin
relates
Prior art date
Application number
PCT/US1999/028588
Other languages
French (fr)
Other versions
WO2000032757A2 (en
Inventor
Rebecca E Cahoon
Sean J Coughlan
Guo-Hua Miao
J Antoni Rafalski
Original Assignee
Du Pont
Rebecca E Cahoon
Sean J Coughlan
Miao Guo Hua
J Antoni Rafalski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Rebecca E Cahoon, Sean J Coughlan, Miao Guo Hua, J Antoni Rafalski filed Critical Du Pont
Priority to AU20377/00A priority Critical patent/AU2037700A/en
Priority to US09/857,613 priority patent/US7071381B1/en
Publication of WO2000032757A2 publication Critical patent/WO2000032757A2/en
Publication of WO2000032757A3 publication Critical patent/WO2000032757A3/en
Priority to US11/051,785 priority patent/US7223905B2/en
Priority to US11/141,593 priority patent/US7226745B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a vitamin E biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the vitamin E biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the vitamin E biosynthetic enzyme in a transformed host cell.
PCT/US1999/028588 1998-12-03 1999-12-02 Plant vitamin e biosynthetic enzymes WO2000032757A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU20377/00A AU2037700A (en) 1998-12-03 1999-12-02 Plant vitamin e biosynthetic enzymes
US09/857,613 US7071381B1 (en) 1998-12-03 1999-12-02 Plant vitamin e biosynthetic enzymes
US11/051,785 US7223905B2 (en) 1998-12-03 2005-02-04 Plant vitamin E biosynthetic enzymes
US11/141,593 US7226745B2 (en) 1998-12-03 2005-05-31 Plant vitamin E biosynthetic enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11078198P 1998-12-03 1998-12-03
US60/110,781 1998-12-03

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US09857613 A-371-Of-International 1999-12-02
US11/051,785 Division US7223905B2 (en) 1998-12-03 2005-02-04 Plant vitamin E biosynthetic enzymes
US11/141,593 Division US7226745B2 (en) 1998-12-03 2005-05-31 Plant vitamin E biosynthetic enzymes

Publications (2)

Publication Number Publication Date
WO2000032757A2 WO2000032757A2 (en) 2000-06-08
WO2000032757A3 true WO2000032757A3 (en) 2000-11-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/028588 WO2000032757A2 (en) 1998-12-03 1999-12-02 Plant vitamin e biosynthetic enzymes

Country Status (2)

Country Link
AU (1) AU2037700A (en)
WO (1) WO2000032757A2 (en)

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Patent Citations (1)

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Non-Patent Citations (5)

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Also Published As

Publication number Publication date
AU2037700A (en) 2000-06-19
WO2000032757A2 (en) 2000-06-08

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