WO2000047726A3 - Plant polyphenol oxidase homologs - Google Patents

Plant polyphenol oxidase homologs Download PDF

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Publication number
WO2000047726A3
WO2000047726A3 PCT/US2000/003176 US0003176W WO0047726A3 WO 2000047726 A3 WO2000047726 A3 WO 2000047726A3 US 0003176 W US0003176 W US 0003176W WO 0047726 A3 WO0047726 A3 WO 0047726A3
Authority
WO
WIPO (PCT)
Prior art keywords
polyphenol oxidase
oxidase enzyme
plant polyphenol
relates
chimeric gene
Prior art date
Application number
PCT/US2000/003176
Other languages
French (fr)
Other versions
WO2000047726A2 (en
Inventor
Rebecca E Cahoon
Saverio Carl Falco
Anthony J Kinney
Guo-Hua Miao
Original Assignee
Du Pont
Rebecca E Cahoon
Saverio Carl Falco
Anthony J Kinney
Miao Guo Hua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Rebecca E Cahoon, Saverio Carl Falco, Anthony J Kinney, Miao Guo Hua filed Critical Du Pont
Priority to US09/889,463 priority Critical patent/US6680185B1/en
Priority to EP00911722A priority patent/EP1151084A2/en
Priority to AU33576/00A priority patent/AU3357600A/en
Publication of WO2000047726A2 publication Critical patent/WO2000047726A2/en
Publication of WO2000047726A3 publication Critical patent/WO2000047726A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0059—Catechol oxidase (1.10.3.1), i.e. tyrosinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a polyphenol oxidase enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the polyphenol oxidase enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the polyphenol oxidase enzyme in a transformed host cell.
PCT/US2000/003176 1999-02-10 2000-02-08 Plant polyphenol oxidase homologs WO2000047726A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US09/889,463 US6680185B1 (en) 1999-02-10 2000-02-08 Plant polyphenol oxidase homologs
EP00911722A EP1151084A2 (en) 1999-02-10 2000-02-08 Plant polyphenol oxidase homologs
AU33576/00A AU3357600A (en) 1999-02-10 2000-02-08 Plant polyphenol oxidase homologs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11959099P 1999-02-10 1999-02-10
US60/119,590 1999-02-10

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/889,463 A-371-Of-International US6680185B1 (en) 1999-02-10 2000-02-08 Plant polyphenol oxidase homologs
US10/727,936 Division US20040214201A1 (en) 1999-02-10 2003-12-03 Plant polyphenol oxidase homologs

Publications (2)

Publication Number Publication Date
WO2000047726A2 WO2000047726A2 (en) 2000-08-17
WO2000047726A3 true WO2000047726A3 (en) 2001-03-08

Family

ID=22385207

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/003176 WO2000047726A2 (en) 1999-02-10 2000-02-08 Plant polyphenol oxidase homologs

Country Status (3)

Country Link
EP (1) EP1151084A2 (en)
AU (1) AU3357600A (en)
WO (1) WO2000047726A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111218434B (en) * 2020-03-09 2022-01-04 山东省农业科学院作物研究所 Wheat grain polyphenol oxidase gene Ppo1 mutant and application thereof

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112048569B (en) * 2020-10-26 2022-08-02 江苏省农业科学院 Molecular marker coseparated with wheat low polyphenol oxidase activity gene QPpo-5D
CN114703220A (en) * 2022-02-11 2022-07-05 新疆农业科学院农产品贮藏加工研究所 Grape fruit stem polyphenol oxidase VvPPO1 subcellular localization detection research method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993002195A1 (en) * 1991-07-17 1993-02-04 Commonwealth Scientific And Industrial Research Organisation Polyphenol oxidase genes
WO1993015599A1 (en) * 1992-01-31 1993-08-19 Cornell Research Foundation, Inc. Polyphenol oxidase
WO1997029193A1 (en) * 1996-02-05 1997-08-14 Commonwealth Scientific And Industrial Research Organisation Genomic ppo clones
WO1998053080A1 (en) * 1997-05-19 1998-11-26 Commonwealth Scientific And Industrial Research Organisation Polyphenol oxidase genes from banana, tobacco and pineapple

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993002195A1 (en) * 1991-07-17 1993-02-04 Commonwealth Scientific And Industrial Research Organisation Polyphenol oxidase genes
WO1993015599A1 (en) * 1992-01-31 1993-08-19 Cornell Research Foundation, Inc. Polyphenol oxidase
WO1997029193A1 (en) * 1996-02-05 1997-08-14 Commonwealth Scientific And Industrial Research Organisation Genomic ppo clones
WO1998053080A1 (en) * 1997-05-19 1998-11-26 Commonwealth Scientific And Industrial Research Organisation Polyphenol oxidase genes from banana, tobacco and pineapple

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111218434B (en) * 2020-03-09 2022-01-04 山东省农业科学院作物研究所 Wheat grain polyphenol oxidase gene Ppo1 mutant and application thereof

Also Published As

Publication number Publication date
WO2000047726A2 (en) 2000-08-17
AU3357600A (en) 2000-08-29
EP1151084A2 (en) 2001-11-07

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