SG10201408300PA - Processing of amplified dna fragments for sequencing - Google Patents

Processing of amplified dna fragments for sequencing

Info

Publication number
SG10201408300PA
SG10201408300PA SG10201408300PA SG10201408300PA SG10201408300PA SG 10201408300P A SG10201408300P A SG 10201408300PA SG 10201408300P A SG10201408300P A SG 10201408300PA SG 10201408300P A SG10201408300P A SG 10201408300PA SG 10201408300P A SG10201408300P A SG 10201408300PA
Authority
SG
Singapore
Prior art keywords
sequencing
dna
processing
dna fragments
amplified dna
Prior art date
Application number
SG10201408300PA
Inventor
Masafumi Muratani
Huck Hui Ng
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Priority to SG10201408300PA priority Critical patent/SG10201408300PA/en
Publication of SG10201408300PA publication Critical patent/SG10201408300PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Abstract

23 Abstract Processing of Amplified DNA Fragments for Sequencing A processing method to trim ends DNA of fragments, exposing the internal DNA part to give original DNA sequence information enabling application next generation of sequencing for DNA samples to be amplified by DOP-PCR or other primer-dependent amplification methods. Figure 8
SG10201408300PA 2009-12-15 2010-12-15 Processing of amplified dna fragments for sequencing SG10201408300PA (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SG10201408300PA SG10201408300PA (en) 2009-12-15 2010-12-15 Processing of amplified dna fragments for sequencing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG2009083429 2009-12-15
SG10201408300PA SG10201408300PA (en) 2009-12-15 2010-12-15 Processing of amplified dna fragments for sequencing

Publications (1)

Publication Number Publication Date
SG10201408300PA true SG10201408300PA (en) 2015-01-29

Family

ID=54193738

Family Applications (2)

Application Number Title Priority Date Filing Date
SG2012041661A SG181543A1 (en) 2009-12-15 2010-12-15 Processing of amplified dna fragments for sequencing
SG10201408300PA SG10201408300PA (en) 2009-12-15 2010-12-15 Processing of amplified dna fragments for sequencing

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG2012041661A SG181543A1 (en) 2009-12-15 2010-12-15 Processing of amplified dna fragments for sequencing

Country Status (4)

Country Link
US (1) US9506110B2 (en)
DE (1) DE112010004821T5 (en)
SG (2) SG181543A1 (en)
WO (1) WO2011075083A1 (en)

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DE112010004821T5 (en) 2009-12-15 2012-10-04 Agency For Science, Technology And Research Processing of amplified DNA fragments for sequencing
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
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CN109996875B (en) * 2016-08-24 2023-11-28 注入技术公司 Method for concentrating microorganisms or extracting nucleic acids using DTBP
CN108504649B (en) * 2017-02-24 2021-03-05 金弗康生物科技(上海)股份有限公司 Coding PCR second-generation sequencing database building method, kit and detection method
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3894591A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
DK3891300T3 (en) 2019-12-23 2023-05-22 10X Genomics Inc METHODS FOR SPATIAL ANALYSIS USING RNA TEMPLATE LIGATION
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4139508A1 (en) * 2020-04-20 2023-03-01 Myriad Genetics, Inc. Comprehensive polygenic risk prediction for breast cancer
CN115916999A (en) 2020-04-22 2023-04-04 10X基因组学有限公司 Methods for spatial analysis using targeted RNA depletion
EP4153775A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
AU2021283174A1 (en) * 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207A1 (en) 2020-06-10 2023-04-19 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array

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US6413758B1 (en) * 2000-10-20 2002-07-02 New England Biolabs, Inc. Method for cloning and expression of Bpml restriction endonuclease in E. coli
CA2477611A1 (en) * 2002-03-01 2003-09-12 Ravgen, Inc. Rapid analysis of variations in a genome
US7135310B2 (en) * 2002-04-24 2006-11-14 The Regents Of The University Of California Method to amplify variable sequences without imposing primer sequences
WO2007149789A2 (en) 2006-06-22 2007-12-27 Applera Corporation Conversion of target specific amplification to universal sequencing
DE112010004821T5 (en) 2009-12-15 2012-10-04 Agency For Science, Technology And Research Processing of amplified DNA fragments for sequencing

Also Published As

Publication number Publication date
SG181543A1 (en) 2012-07-30
WO2011075083A1 (en) 2011-06-23
DE112010004821T5 (en) 2012-10-04
US9506110B2 (en) 2016-11-29
US20120252702A1 (en) 2012-10-04

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