ATE113653T1 - Verfahren zur klonierung eines restriktionsmodifizierungssystems. - Google Patents

Verfahren zur klonierung eines restriktionsmodifizierungssystems.

Info

Publication number
ATE113653T1
ATE113653T1 AT87305005T AT87305005T ATE113653T1 AT E113653 T1 ATE113653 T1 AT E113653T1 AT 87305005 T AT87305005 T AT 87305005T AT 87305005 T AT87305005 T AT 87305005T AT E113653 T1 ATE113653 T1 AT E113653T1
Authority
AT
Austria
Prior art keywords
gene
cloning
restriction
methods
modification system
Prior art date
Application number
AT87305005T
Other languages
English (en)
Inventor
Joan Ellen Brooks
Kimberly Anne Howard
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of ATE113653T1 publication Critical patent/ATE113653T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Paper (AREA)
  • Electrotherapy Devices (AREA)
  • Use Of Switch Circuits For Exchanges And Methods Of Control Of Multiplex Exchanges (AREA)
  • Control Of Eletrric Generators (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT87305005T 1986-06-06 1987-06-05 Verfahren zur klonierung eines restriktionsmodifizierungssystems. ATE113653T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US87204686A 1986-06-06 1986-06-06

Publications (1)

Publication Number Publication Date
ATE113653T1 true ATE113653T1 (de) 1994-11-15

Family

ID=25358720

Family Applications (1)

Application Number Title Priority Date Filing Date
AT87305005T ATE113653T1 (de) 1986-06-06 1987-06-05 Verfahren zur klonierung eines restriktionsmodifizierungssystems.

Country Status (7)

Country Link
US (2) US5137823A (de)
EP (3) EP0248678B1 (de)
JP (1) JP2647847B2 (de)
CN (1) CN87104039A (de)
AT (1) ATE113653T1 (de)
AU (1) AU607141B2 (de)
DE (3) DE3750708T2 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
DE3854373T2 (de) * 1987-10-15 1996-03-07 New England Biolabs Inc Verfahren zur Herstellung der Afl II Restriktionsendonuklease.
US4999293A (en) * 1987-12-17 1991-03-12 New England Biolabs, Inc. Method for producing the HhaI restriction endonuclease and methylase
US4983522A (en) * 1987-12-17 1991-01-08 New England Biolabs, Inc. Method for producing the HinPI restriction endonuclease and methylase
JPH022365A (ja) * 1987-12-17 1990-01-08 New England Biolabs Inc ＢａｎＩ制限エンドヌクレアーゼおよびメチラーゼの製造方法
US4987074A (en) * 1987-12-17 1991-01-22 New England Biolabs, Inc. Method for producing the HgiAI restriction endonuclease and methylase
US4988620A (en) * 1987-12-17 1991-01-29 New England Biolabs, Inc. Method for producing the FnuDI restriction endonuclease and methylase
US4999294A (en) * 1987-12-17 1991-03-12 New England Biolabs, Inc. Method for producing the FokI restriction endonuclease and methylase
US4983542A (en) * 1987-12-21 1991-01-08 New England Biolabs, Inc. Method for producing the XbaI restriction endonuclease and methylase
EP0341776A3 (de) * 1988-05-09 1990-07-25 Merck & Co. Inc. Verfahren zur Aufnahme von DNS-Moleküle in Mikroorganismen mit Methyl-spezifischen Restriktionsystemen
US5194387A (en) * 1988-05-09 1993-03-16 Merck & Co., Inc. Process for the incorporation of DNA molecules into microorganisms with methyl-specific restriction systems
US5139942A (en) * 1988-05-19 1992-08-18 New England Biolabs, Inc. Method for producing the nde i restriction endonuclease and methylase
JPH07121219B2 (ja) * 1988-08-22 1995-12-25 東洋紡績株式会社 新規なＢａｍＨＩ制限エンドヌクレアーゼの製造方法
US5298404A (en) * 1989-10-13 1994-03-29 New England Biolabs, Inc. Method for producing the Hpa I restriction endonuclease and methylase
DE4018441A1 (de) * 1990-06-08 1991-12-12 Boehringer Mannheim Gmbh Rekombinantes restriktionsenzym sau3ai
US5278060A (en) * 1990-08-30 1994-01-11 New England Biolabs, Inc. Method for producing the Nla III restriction endonuclease and methylase
US5288696A (en) * 1990-09-07 1994-02-22 New England Biolabs, Inc. Method for producing and cloning SacII restriction endonuclease and methylase
US5202248A (en) * 1990-11-02 1993-04-13 New England Biolabs, Inc. Method for cloning and producing the nco i restriction endonuclease and methylase
US5196330A (en) * 1991-06-03 1993-03-23 New England Biolabs, Inc. Type ii restriction endonuclease, pme i, obtainable from pseudomonas mendocina and a process for producing the same
US5231021A (en) * 1992-04-10 1993-07-27 Life Technologies, Inc. Cloning and expressing restriction endonucleases and modification methylases from xanthomonas
US5472872A (en) * 1994-01-13 1995-12-05 Mead; David Recombinant CviJI restriction endonuclease
US5434068A (en) * 1993-04-09 1995-07-18 New England Biolabs, Inc. Method for cloning and producing the BglII restriction endonuclease and modification methylase
JP3836168B2 (ja) * 1995-02-03 2006-10-18 タカラバイオ株式会社 ＢａｌＩ制限・修飾系酵素遺伝子
JP3549210B2 (ja) 1996-03-13 2004-08-04 タカラバイオ株式会社 プラスミド
US5945326A (en) * 1997-03-20 1999-08-31 New England Biolabs, Inc. Method for cloning and producing the Spel restriction endonuclease
US6048731A (en) * 1998-09-03 2000-04-11 New England Biolabs, Inc. Method for cloning and producing the SgrAI restriction endonuclease
US6048719A (en) * 1999-01-22 2000-04-11 New England Biolabs, Inc. Method for cloning and producing the DraIII restriction endonuclease
US6245545B1 (en) 1999-04-27 2001-06-12 New England Biolabs, Inc. Method for cloning and producing the SwaI restriction endonuclease
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
US6846658B1 (en) * 2000-10-12 2005-01-25 New England Biolabs, Inc. Method for cloning and producing the Msel restriction endonuclease
US6335190B1 (en) * 2000-10-20 2002-01-01 New England Biolabs, Inc. Method for cloning and producing the BsmI restriction endonuclease in E. coli
US6413758B1 (en) * 2000-10-20 2002-07-02 New England Biolabs, Inc. Method for cloning and expression of Bpml restriction endonuclease in E. coli
US20030211506A1 (en) * 2001-06-01 2003-11-13 Huimin Kong N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
US7081358B2 (en) 2001-08-23 2006-07-25 New England Biolabs, Inc. Method for engineering strand-specific, sequence-specific, DNA-nicking enzymes
US6764843B2 (en) * 2001-09-28 2004-07-20 New England Biolabs, Inc. Method of cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease
CA2541177A1 (en) * 2003-10-03 2005-09-22 Promega Corporation Vectors for directional cloning
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
KR100683852B1 (ko) * 2004-07-02 2007-02-15 삼성전자주식회사 반도체 소자의 마스크롬 소자 및 그 형성 방법
EP2548953A1 (de) 2005-08-04 2013-01-23 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
WO2007103061A2 (en) * 2006-03-08 2007-09-13 New England Biolabs, Inc. Method for cloning and expression of stui restriction endonuclease and stui methylase in e. coli
CN101595214B (zh) 2006-11-29 2013-06-12 诺维信股份有限公司 改进向细菌细胞中导入dna的方法
CN101225396B (zh) * 2008-02-04 2011-11-30 金普诺安蛋白质工程技术（北京）有限公司 利用基因工程生产限制性内切酶的方法
US11066653B2 (en) 2017-07-21 2021-07-20 Basf Se Method of transforming bacterial cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4530904A (en) * 1982-09-03 1985-07-23 Eli Lilly And Company Method for conferring bacteriophage resistance to bacteria

Also Published As

Publication number Publication date
EP0618295B1 (de) 1996-03-06
EP0618295A1 (de) 1994-10-05
EP0248678B1 (de) 1994-11-02
DE3751730D1 (de) 1996-04-11
DE3750708T2 (de) 1995-06-29
JP2647847B2 (ja) 1997-08-27
EP0618296B1 (de) 1996-10-09
JPS6387982A (ja) 1988-04-19
DE3751923D1 (de) 1996-11-14
US5320957A (en) 1994-06-14
AU607141B2 (en) 1991-02-28
EP0618296A1 (de) 1994-10-05
EP0248678A2 (de) 1987-12-09
US5137823A (en) 1992-08-11
AU7389287A (en) 1988-01-07
DE3751730T2 (de) 1996-09-12
CN87104039A (zh) 1988-03-23
DE3750708D1 (de) 1994-12-08
DE3751923T2 (de) 1997-04-03
EP0248678A3 (en) 1988-02-24

Similar Documents

Publication Publication Date Title
ATE113653T1 (de) Verfahren zur klonierung eines restriktionsmodifizierungssystems.
DE3163939D1 (en) Method for enzymatic interesterification of lipid and enzyme used therein
IL58244A0 (en) Method and apparatus for determining the minimum concentration of antibiotic to inhibit micro-organism growth
DE3773791D1 (de) Verfahren zur partialoxydation eines kohlenwasserstoffbrennstoffs.
DE3688163D1 (de) Verfahren zur rueckgewinnung von extrazellulaeren enzymen aus gesamten fermentationsbruehen.
DE3574799D1 (de) Verfahren zur vakuumentgasung und anfuellung eines reaktorkuehlsystems.
DE3874889D1 (de) Verfahren zur wiedergabe von sichtbildern eines objektes.
DE3888804D1 (de) Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase.
DE3672920D1 (de) Verfahren zur herstellung immobilisierter enzyme und vorrichtung zur durchfuehrung des verfahrens.
ATE118817T1 (de) Verfahren zur herstellung von xbai- restriktionsendonuklease und -methylase.
IL65572A (en) Method for transformation of microorganisms
DK148827C (da) Fremgangsmaade til fremstilling af cholesterolesterase samt fremgangsmaade til hydrolyse af cholesterolestere
ATE194329T1 (de) Verfahren zur stereospezifischen hydrolyse von piperidin-dion derivaten
DE69006489D1 (de) Verfahren zur kontinuierlichen wiedergewinnung von fluorwasserstoffgas.
DE3761579D1 (de) Verfahren zur kraftstoffzuteilung.
DE59007810D1 (de) Verfahren zur enzymatischen Spaltung von 2-Arylpropionsäurevinylestern.
DE3888648D1 (de) Verfahren zur Herstellung von HgiAI-Restriktionsendonuklease und -methylase.
DE3889884D1 (de) Verfahren zur Herstellung von HinPI-Restriktionsendonuklease und -methylase.
ATA423283A (de) Verfahren zur modifizierten enzymatischen hydrolyse von proteinsubstraten
ATA592480A (de) Verfahren zur enzymatischen schlammstabilisierung
ATA88691A (de) Verfahren zur enzymatischen hydrolyse eines carbonsäurederivates
AT388568B (de) Verfahren zur verringerung des einschlussgehaltes von staehlen sowie zur verfeinerung ihres gefueges
DE3770177D1 (de) Halbacetale von glyoxylsaeureestern sowie verfahren zur isolierung von glyoxylsaeureestern.
DE3761611D1 (de) Verfahren zur abklaerung und stabilisierung eines zellkulturmediums.
ATE118816T1 (de) Verfahren zur herstellung von acci- restriktionsendonuklease und -methylase.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties