DE3888804D1 - Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase. - Google Patents

Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase.

Info

Publication number
DE3888804D1
DE3888804D1 DE88311917T DE3888804T DE3888804D1 DE 3888804 D1 DE3888804 D1 DE 3888804D1 DE 88311917 T DE88311917 T DE 88311917T DE 3888804 T DE3888804 T DE 3888804T DE 3888804 D1 DE3888804 D1 DE 3888804D1
Authority
DE
Germany
Prior art keywords
restriction endonuclease
foki restriction
methylase
production
foki
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE88311917T
Other languages
English (en)
Other versions
DE3888804T2 (de
Inventor
Mary Looney
Geoffrey Wilson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of DE3888804D1 publication Critical patent/DE3888804D1/de
Publication of DE3888804T2 publication Critical patent/DE3888804T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE3888804T 1987-12-17 1988-12-16 Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase. Expired - Lifetime DE3888804T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/134,236 US4999294A (en) 1987-12-17 1987-12-17 Method for producing the FokI restriction endonuclease and methylase

Publications (2)

Publication Number Publication Date
DE3888804D1 true DE3888804D1 (de) 1994-05-05
DE3888804T2 DE3888804T2 (de) 1994-11-10

Family

ID=22462394

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3888804T Expired - Lifetime DE3888804T2 (de) 1987-12-17 1988-12-16 Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase.

Country Status (5)

Country Link
US (1) US4999294A (de)
EP (1) EP0321266B1 (de)
JP (1) JPH022370A (de)
AT (1) ATE103637T1 (de)
DE (1) DE3888804T2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5238821A (en) * 1989-05-16 1993-08-24 Genzyme Corporation Endo F-free PNGase
US5298404A (en) * 1989-10-13 1994-03-29 New England Biolabs, Inc. Method for producing the Hpa I restriction endonuclease and methylase
US5192676A (en) * 1991-02-05 1993-03-09 New England Biolabs, Inc. Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same
US5200337A (en) * 1991-10-25 1993-04-06 New England Biolabs, Inc. Type ii restriction endonuclease, apo i, obtainable from arthrobacter protophormiae and a process for producing the same
DE69514791T2 (de) 1995-07-24 2000-07-20 Stmicroelectronics S.R.L., Agrate Brianza Flash-EEPROM mit onchip-Löschung-Source-Spannungsgenerator
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
EP2548953A1 (de) 2005-08-04 2013-01-23 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
AU2011338841B2 (en) 2010-11-12 2017-02-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP2748318B1 (de) 2011-08-26 2015-11-04 Gen9, Inc. Zusammensetzungen und verfahren für hochqualitative anordnung von nukleinsäuren
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
LT2841601T (lt) 2012-04-24 2019-07-10 Gen9, Inc. Nukleorūgščių rūšiavimo būdai ir multipleksinis preparatyvinis in vitro klonavimas
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
DE3750708T2 (de) * 1986-06-06 1995-06-29 New England Biolabs Inc Verfahren zur Klonierung eines Restriktionsmodifizierungssystems.

Also Published As

Publication number Publication date
JPH022370A (ja) 1990-01-08
ATE103637T1 (de) 1994-04-15
DE3888804T2 (de) 1994-11-10
EP0321266B1 (de) 1994-03-30
EP0321266A3 (en) 1990-03-21
US4999294A (en) 1991-03-12
EP0321266A2 (de) 1989-06-21

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