DE3888804T2 - Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase. - Google Patents
Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase.Info
- Publication number
- DE3888804T2 DE3888804T2 DE3888804T DE3888804T DE3888804T2 DE 3888804 T2 DE3888804 T2 DE 3888804T2 DE 3888804 T DE3888804 T DE 3888804T DE 3888804 T DE3888804 T DE 3888804T DE 3888804 T2 DE3888804 T2 DE 3888804T2
- Authority
- DE
- Germany
- Prior art keywords
- restriction endonuclease
- foki restriction
- methylase
- production
- foki
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1007—Methyltransferases (general) (2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/134,236 US4999294A (en) | 1987-12-17 | 1987-12-17 | Method for producing the FokI restriction endonuclease and methylase |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3888804D1 DE3888804D1 (de) | 1994-05-05 |
DE3888804T2 true DE3888804T2 (de) | 1994-11-10 |
Family
ID=22462394
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE3888804T Expired - Lifetime DE3888804T2 (de) | 1987-12-17 | 1988-12-16 | Verfahren zur Herstellung von FokI-Restriktionsendonuklease und -methylase. |
Country Status (5)
Country | Link |
---|---|
US (1) | US4999294A (de) |
EP (1) | EP0321266B1 (de) |
JP (1) | JPH022370A (de) |
AT (1) | ATE103637T1 (de) |
DE (1) | DE3888804T2 (de) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5238821A (en) * | 1989-05-16 | 1993-08-24 | Genzyme Corporation | Endo F-free PNGase |
US5298404A (en) * | 1989-10-13 | 1994-03-29 | New England Biolabs, Inc. | Method for producing the Hpa I restriction endonuclease and methylase |
US5192676A (en) * | 1991-02-05 | 1993-03-09 | New England Biolabs, Inc. | Type ii restriction endonuclease, asci, obtainable from arthrobacter species and a process for producing the same |
US5200337A (en) * | 1991-10-25 | 1993-04-06 | New England Biolabs, Inc. | Type ii restriction endonuclease, apo i, obtainable from arthrobacter protophormiae and a process for producing the same |
EP0756286B1 (de) | 1995-07-24 | 2000-01-26 | STMicroelectronics S.r.l. | Flash-EEPROM mit onchip-Löschung-Source-Spannungsgenerator |
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
EP2574668A1 (de) | 2005-08-04 | 2013-04-03 | New England Biolabs, Inc. | Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität |
US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
US10207240B2 (en) | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
WO2011066185A1 (en) | 2009-11-25 | 2011-06-03 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
WO2011085075A2 (en) | 2010-01-07 | 2011-07-14 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
EP4039363A1 (de) | 2010-11-12 | 2022-08-10 | Gen9, Inc. | Proteinanordnungen und verfahren zu ihrer herstellung und verwendung |
CA3132011A1 (en) | 2010-11-12 | 2012-06-14 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
EP3954770A1 (de) | 2011-08-26 | 2022-02-16 | Gen9, Inc. | Zusammensetzungen und verfahren zur hochqualitativen anordnung von nukleinsäuren |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
US10081807B2 (en) | 2012-04-24 | 2018-09-25 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
IL236303B (en) | 2012-06-25 | 2022-07-01 | Gen9 Inc | Methods for high-throughput nucleic acid assembly and sequencing |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IN166864B (de) * | 1985-03-01 | 1990-07-28 | New England Biolabs Inc | |
EP0618295B1 (de) * | 1986-06-06 | 1996-03-06 | New England Biolabs, Inc. | Verfahren zur Klonierung des Dde I- Restriktions-Modifikationssystems |
-
1987
- 1987-12-17 US US07/134,236 patent/US4999294A/en not_active Expired - Lifetime
-
1988
- 1988-12-16 DE DE3888804T patent/DE3888804T2/de not_active Expired - Lifetime
- 1988-12-16 EP EP88311917A patent/EP0321266B1/de not_active Expired - Lifetime
- 1988-12-16 JP JP63319327A patent/JPH022370A/ja active Pending
- 1988-12-16 AT AT88311917T patent/ATE103637T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP0321266A2 (de) | 1989-06-21 |
EP0321266A3 (en) | 1990-03-21 |
EP0321266B1 (de) | 1994-03-30 |
ATE103637T1 (de) | 1994-04-15 |
JPH022370A (ja) | 1990-01-08 |
DE3888804D1 (de) | 1994-05-05 |
US4999294A (en) | 1991-03-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |