DK153992D0 - Metode - Google Patents

Metode

Info

Publication number
DK153992D0
DK153992D0 DK921539A DK153992A DK153992D0 DK 153992 D0 DK153992 D0 DK 153992D0 DK 921539 A DK921539 A DK 921539A DK 153992 A DK153992 A DK 153992A DK 153992 D0 DK153992 D0 DK 153992D0
Authority
DK
Denmark
Prior art keywords
sequence
dna sequence
dna
genome
cell
Prior art date
Application number
DK921539A
Other languages
English (en)
Inventor
Steen Troels Joergensen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Priority to DK921539A priority Critical patent/DK153992D0/da
Publication of DK153992D0 publication Critical patent/DK153992D0/da
Priority to JP51470694A priority patent/JP3983798B2/ja
Priority to PCT/DK1993/000438 priority patent/WO1994014968A1/en
Priority to AU56944/94A priority patent/AU679707B2/en
Priority to EP94902652A priority patent/EP0675959B1/en
Priority to CA002152371A priority patent/CA2152371C/en
Priority to AT94902652T priority patent/ATE323167T1/de
Priority to DK94902652T priority patent/DK0675959T3/da
Priority to DE69334006T priority patent/DE69334006T2/de
Priority to CN94109005A priority patent/CN1063488C/zh
Priority to NO952496A priority patent/NO952496L/no
Priority to FI953079A priority patent/FI120741B/fi
Priority to US08/751,282 priority patent/US5733753A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Semiconductor Lasers (AREA)
DK921539A 1992-12-22 1992-12-22 Metode DK153992D0 (da)

Priority Applications (13)

Application Number Priority Date Filing Date Title
DK921539A DK153992D0 (da) 1992-12-22 1992-12-22 Metode
DE69334006T DE69334006T2 (de) 1992-12-22 1993-12-22 Dna - amplifikation
EP94902652A EP0675959B1 (en) 1992-12-22 1993-12-22 Dna amplification
PCT/DK1993/000438 WO1994014968A1 (en) 1992-12-22 1993-12-22 Dna amplification
AU56944/94A AU679707B2 (en) 1992-12-22 1993-12-22 DNA amplification
JP51470694A JP3983798B2 (ja) 1992-12-22 1993-12-22 Ｄｎａ増幅
CA002152371A CA2152371C (en) 1992-12-22 1993-12-22 Dna amplification
AT94902652T ATE323167T1 (de) 1992-12-22 1993-12-22 Dna - amplifikation
DK94902652T DK0675959T3 (da) 1992-12-22 1993-12-22 DNA amplificering
CN94109005A CN1063488C (zh) 1992-12-22 1994-06-22 Dna扩增
NO952496A NO952496L (no) 1992-12-22 1995-06-21 DNA-amplifikasjon
FI953079A FI120741B (fi) 1992-12-22 1995-06-21 DNA:n vahvistaminen
US08/751,282 US5733753A (en) 1992-12-22 1996-11-18 Amplification of genomic DNA by site specific integration of a selectable marker construct

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK921539A DK153992D0 (da) 1992-12-22 1992-12-22 Metode
CN94109005A CN1063488C (zh) 1992-12-22 1994-06-22 Dna扩增

Publications (1)

Publication Number Publication Date
DK153992D0 true DK153992D0 (da) 1992-12-22

Family

ID=37075831

Family Applications (2)

Application Number Title Priority Date Filing Date
DK921539A DK153992D0 (da) 1992-12-22 1992-12-22 Metode
DK94902652T DK0675959T3 (da) 1992-12-22 1993-12-22 DNA amplificering

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK94902652T DK0675959T3 (da) 1992-12-22 1993-12-22 DNA amplificering

Country Status (11)

Country Link
EP (1) EP0675959B1 (da)
JP (1) JP3983798B2 (da)
CN (1) CN1063488C (da)
AT (1) ATE323167T1 (da)
AU (1) AU679707B2 (da)
CA (1) CA2152371C (da)
DE (1) DE69334006T2 (da)
DK (2) DK153992D0 (da)
FI (1) FI120741B (da)
NO (1) NO952496L (da)
WO (1) WO1994014968A1 (da)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994021787A1 (en) * 1993-03-15 1994-09-29 Cell Genesys, Inc. Method for defined deletions of dna
EP0817856A1 (en) * 1995-03-22 1998-01-14 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
FR2762842B1 (fr) * 1997-05-05 1999-07-23 Pasteur Institut Procede de detection de la presence de matieres biologiques d'origine bovine, et oligonucleotides pour sa mise en oeuvre
CN1261918A (zh) * 1997-07-03 2000-08-02 诺沃挪第克公司 具有多拷贝目的基因和可筛选标记而不具选择性标记的产蛋白质细胞
EP1041890B1 (en) 1997-12-22 2005-04-20 Novozymes A/S Carbohydrate oxidase and use thereof in baking
WO2002000907A1 (en) 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
DK1995319T3 (da) 2002-04-10 2011-12-05 Novozymes As Forbedret bacillusværtscelle
EP2213745A1 (en) 2002-04-10 2010-08-04 Novozymes A/S Improved bacillus host cell
US7700322B2 (en) 2003-10-31 2010-04-20 Novozymes A/S Method for stable gene-amplification in a bacterial host cell
US20070249016A1 (en) * 2003-11-25 2007-10-25 Japan Science And Technology Agency Method for Gene Amplification
US20080044853A1 (en) 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
CN101313064B (zh) * 2005-11-24 2012-11-28 大学共同利用机关法人自然科学研究机构 基因扩增方法
CN101595214B (zh) 2006-11-29 2013-06-12 诺维信股份有限公司 改进向细菌细胞中导入dna的方法
EP2104739B1 (en) 2006-12-21 2013-06-19 Novozymes Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells
WO2010097436A1 (en) 2009-02-27 2010-09-02 Novozymes A/S Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
US20120329090A1 (en) 2009-12-21 2012-12-27 Novozymes A/S Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells
WO2012159007A1 (en) 2011-05-19 2012-11-22 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis
CN107287331B (zh) * 2017-08-01 2020-06-26 北京大学 一种高效检测蓝藻细胞周期的方法
WO2019092042A1 (en) 2017-11-10 2019-05-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
EP3918086A1 (en) 2019-01-30 2021-12-08 Novozymes A/S Cognate foldase co-expression
US20220170003A1 (en) 2019-03-08 2022-06-02 Novozymes A/S Means And Methods For Improving Protease Expression
US20220275354A1 (en) 2019-05-15 2022-09-01 Novozymes A/S TEMPERATURE-SENSITIVE RNA- Guided Endonuclease
US20220298517A1 (en) 2019-06-25 2022-09-22 Novozymes A/S Counter-selection by inhibition of conditionally essential genes
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression
WO2025132815A1 (en) 2023-12-20 2025-06-26 Novozymes A/S Novel cas nucleases and polynucleotides encoding the same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
BR8805646A (pt) * 1987-02-27 1989-10-17 Gist Brocades Nv Processo para amplificacao de genes estaveis em adn cromossomico de microorganismos procarioticos
HK1000547A1 (en) * 1989-12-22 1998-04-03 Merck Serono Sa Endogenous gene expression modification with regulatory element by way of homologous recombination

Also Published As

Publication number Publication date
JP3983798B2 (ja) 2007-09-26
FI953079L (fi) 1995-06-21
CA2152371C (en) 2008-12-02
WO1994014968A1 (en) 1994-07-07
JPH08504585A (ja) 1996-05-21
CN1114355A (zh) 1996-01-03
FI120741B (fi) 2010-02-15
FI953079A0 (fi) 1995-06-21
ATE323167T1 (de) 2006-04-15
DE69334006T2 (de) 2006-11-23
NO952496D0 (no) 1995-06-21
CA2152371A1 (en) 1994-07-07
DE69334006D1 (de) 2006-05-24
AU5694494A (en) 1994-07-19
NO952496L (no) 1995-08-21
EP0675959A1 (en) 1995-10-11
CN1063488C (zh) 2001-03-21
EP0675959B1 (en) 2006-04-12
AU679707B2 (en) 1997-07-10
DK0675959T3 (da) 2006-08-07

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Legal Events

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A0 Application filed
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