MX9404063A - Amplificacion simultanea de objetivos multiples. - Google Patents

Amplificacion simultanea de objetivos multiples.

Info

Publication number
MX9404063A
MX9404063A MX9404063A MX9404063A MX9404063A MX 9404063 A MX9404063 A MX 9404063A MX 9404063 A MX9404063 A MX 9404063A MX 9404063 A MX9404063 A MX 9404063A MX 9404063 A MX9404063 A MX 9404063A
Authority
MX
Mexico
Prior art keywords
sequences
amplification
target
sequence
attached
Prior art date
Application number
MX9404063A
Other languages
English (en)
Inventor
George Terrance Walker
James G Nadeau
Michael C Little
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Publication of MX9404063A publication Critical patent/MX9404063A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B19/00—Driving, starting, stopping record carriers not specifically of filamentary or web form, or of supports therefor; Control thereof; Control of operating function ; Driving both disc and head
    • G11B19/02—Control of operating function, e.g. switching from recording to reproducing
    • G11B19/04—Arrangements for preventing, inhibiting, or warning against double recording on the same blank or against other recording or reproducing malfunctions
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B5/00—Recording by magnetisation or demagnetisation of a record carrier; Reproducing by magnetic means; Record carriers therefor
    • G11B5/02—Recording, reproducing, or erasing methods; Read, write or erase circuits therefor
    • G11B5/09—Digital recording
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B5/00—Recording by magnetisation or demagnetisation of a record carrier; Reproducing by magnetic means; Record carriers therefor
    • G11B5/127—Structure or manufacture of heads, e.g. inductive
    • G11B5/187—Structure or manufacture of the surface of the head in physical contact with, or immediately adjacent to the recording medium; Pole pieces; Gap features
    • G11B5/1875—"Composite" pole pieces, i.e. poles composed in some parts of magnetic particles and in some other parts of magnetic metal layers
    • G11B5/1877—"Composite" pole pieces, i.e. poles composed in some parts of magnetic particles and in some other parts of magnetic metal layers including at least one magnetic thin film
    • G11B5/1878—"Composite" pole pieces, i.e. poles composed in some parts of magnetic particles and in some other parts of magnetic metal layers including at least one magnetic thin film disposed immediately adjacent to the transducing gap, e.g. "Metal-In-Gap" structure
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B5/00—Recording by magnetisation or demagnetisation of a record carrier; Reproducing by magnetic means; Record carriers therefor
    • G11B5/127—Structure or manufacture of heads, e.g. inductive
    • G11B5/31—Structure or manufacture of heads, e.g. inductive using thin films
    • G11B5/3109—Details
    • G11B5/3113—Details for improving the magnetic domain structure or avoiding the formation or displacement of undesirable magnetic domains
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B5/00—Recording by magnetisation or demagnetisation of a record carrier; Reproducing by magnetic means; Record carriers therefor
    • G11B5/455—Arrangements for functional testing of heads; Measuring arrangements for heads
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B5/00—Recording by magnetisation or demagnetisation of a record carrier; Reproducing by magnetic means; Record carriers therefor
    • G11B5/465—Arrangements for demagnetisation of heads
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11B—INFORMATION STORAGE BASED ON RELATIVE MOVEMENT BETWEEN RECORD CARRIER AND TRANSDUCER
    • G11B5/00—Recording by magnetisation or demagnetisation of a record carrier; Reproducing by magnetic means; Record carriers therefor
    • G11B2005/0002—Special dispositions or recording techniques
    • G11B2005/0005—Arrangements, methods or circuits
    • G11B2005/0008—Magnetic conditionning of heads, e.g. biasing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Métodos para la amplificación de multicanalización de secuencias de ácidonucleico del objetivo usando un solo par de cebadores. Las secuenciasdefinidas se anexan a los extremos de las secuencias del objetivo múltiplecomo parte de la reacción de amplificación de manera que no se requierenpasos además de la amplificación. Las secuencias del objetivo con lassecuencias definidas anexas no necesitan aislarse antes de laamplificación. En una modalidad para la co-amplificación de dos secuenciasdel objetivo, se anexa una secuencia que corresponde a un segmento de laprimera secuencia del objetivo a un extremo de la segunda secuencia elobjetivo y una secuencia que corresponde a un segmento terminal de lasegunda secuencia del objetivo se anexa a un extremo de la primerasecuencia del objetivo. La amplificación de los dos objetivos luegorequiere solamente un solo par de cebadores. Alternativamente, se puedeanexar una secuencia definida individual a los extremos 5' y 3' decualquier número de objetivos seleccionados. Toadas estas secuencias delobjetivo modificadas pueden amplificarse usando un solo par de cebadoresque se hibridizan en las secuencias de extremo definidas.
MX9404063A 1993-06-04 1994-05-31 Amplificacion simultanea de objetivos multiples. MX9404063A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/073,197 US5422252A (en) 1993-06-04 1993-06-04 Simultaneous amplification of multiple targets

Publications (1)

Publication Number Publication Date
MX9404063A true MX9404063A (es) 1995-01-31

Family

ID=22112324

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9404063A MX9404063A (es) 1993-06-04 1994-05-31 Amplificacion simultanea de objetivos multiples.

Country Status (10)

Country Link
US (2) US5422252A (es)
EP (1) EP0628640B1 (es)
JP (1) JP2703183B2 (es)
AU (1) AU673424B2 (es)
BR (1) BR9402174A (es)
CA (1) CA2125004C (es)
DE (1) DE69412540T2 (es)
ES (1) ES2122083T3 (es)
MX (1) MX9404063A (es)
SG (1) SG52767A1 (es)

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US5624825A (en) 1997-04-29
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AU6453794A (en) 1994-12-08
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