EA200300647A1 - Изотермическая амплификация нуклеиновых кислот на твердой подложке - Google Patents

Изотермическая амплификация нуклеиновых кислот на твердой подложке

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Publication number
EA200300647A1
EA200300647A1 EA200300647A EA200300647A EA200300647A1 EA 200300647 A1 EA200300647 A1 EA 200300647A1 EA 200300647 A EA200300647 A EA 200300647A EA 200300647 A EA200300647 A EA 200300647A EA 200300647 A1 EA200300647 A1 EA 200300647A1
Authority
EA
Eurasian Patent Office
Prior art keywords
nucleic acids
isothermal amplification
solid substrate
methods
applicable
Prior art date
Application number
EA200300647A
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English (en)
Inventor
Паскаль Майер
Original Assignee
Апплайд Резеч Системз Арс Холдинг Н.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Апплайд Резеч Системз Арс Холдинг Н.В. filed Critical Апплайд Резеч Системз Арс Холдинг Н.В.
Publication of EA200300647A1 publication Critical patent/EA200300647A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

Описаны способы для изотермической амплификации нуклеиновой кислоты с использованием твердой подложки. Эти способы применимы для приложений, требующих высокой производительности, в частности для секвенирования нуклеиновых кислот.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200300647A 2000-12-08 2001-12-07 Изотермическая амплификация нуклеиновых кислот на твердой подложке EA200300647A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00127011 2000-12-08
PCT/EP2001/014369 WO2002046456A1 (en) 2000-12-08 2001-12-07 Isothermal amplification of nucleic acids on a solid support

Publications (1)

Publication Number Publication Date
EA200300647A1 true EA200300647A1 (ru) 2003-10-30

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EA200300647A EA200300647A1 (ru) 2000-12-08 2001-12-07 Изотермическая амплификация нуклеиновых кислот на твердой подложке

Country Status (21)

Country Link
US (2) US7790418B2 (ru)
EP (1) EP1339877A1 (ru)
JP (1) JP2004524012A (ru)
KR (1) KR20030055343A (ru)
CN (1) CN1489632A (ru)
AR (1) AR031640A1 (ru)
AU (1) AU2002219161A1 (ru)
BG (1) BG107861A (ru)
BR (1) BR0116033A (ru)
CA (1) CA2430369A1 (ru)
CZ (1) CZ20031582A3 (ru)
EA (1) EA200300647A1 (ru)
EE (1) EE200300256A (ru)
HU (1) HUP0302803A2 (ru)
IL (1) IL156311A0 (ru)
MX (1) MXPA03005078A (ru)
NO (1) NO20032455L (ru)
PL (1) PL363742A1 (ru)
SK (1) SK6952003A3 (ru)
WO (1) WO2002046456A1 (ru)
ZA (1) ZA200304102B (ru)

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US20110008781A1 (en) 2011-01-13
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