BRPI0510811A - nano-pcr: métodos e dispositivos para amplificação e detecção de ácido nucléico - Google Patents

nano-pcr: métodos e dispositivos para amplificação e detecção de ácido nucléico

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Publication number
BRPI0510811A
BRPI0510811A BRPI0510811-0A BRPI0510811A BRPI0510811A BR PI0510811 A BRPI0510811 A BR PI0510811A BR PI0510811 A BRPI0510811 A BR PI0510811A BR PI0510811 A BRPI0510811 A BR PI0510811A
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BR
Brazil
Prior art keywords
methods
nucleic acid
pcr
devices
amplification
Prior art date
Application number
BRPI0510811-0A
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English (en)
Inventor
Anita Goel
Original Assignee
Anita Goel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anita Goel filed Critical Anita Goel
Priority to BR122016013290-0A priority Critical patent/BR122016013290B1/pt
Publication of BRPI0510811A publication Critical patent/BRPI0510811A/pt
Publication of BRPI0510811B1 publication Critical patent/BRPI0510811B1/pt

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/0896—Nanoscaled
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/303—Applying a physical force on a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/307—Denaturation or renaturation by electric current/voltage
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/101—Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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Abstract

NANO-PCR: MéTODOS E DISPOSITIVOS PARA AMPLIFICAçãO E DETECçãO DE áCIDO NUCLéICO. São descritos métodos, dispositivos e composições que fornecem a amplificação de seqüências de ácido nucléico sem confiança no ciclo de temperatura, assim liberando os métodos de dispositivos convencionais de ciclos térmicos de benchtop. A desnaturação de ácidos nucléicos de filamento duplo, anelamento de iniciadores, e controle preciso sobre a extensão de iniciador por polimerase pode ser realizado por aplicação de estresse a um ácido nucléico. Esses métodos podem fornecer um ou mais benefícios sobre métodos convencionais de PCR incluindo: a precisão do controle sobre o processo de PCR; fidelidade geralmente melhorada; precisão melhorada sobre seqüências roblemáticas como regiões de repetição ricas em GC e tandem; maior comprimento de seqüência; rendimento aumentado de reação; tempo experimental reduzido; maior eficiência; custo mais baixo; maior portabilidade; e robustez a vários parâmetros ambientais, como temperatura, pH e forças iónicas.
BRPI0510811A 2004-05-13 2005-05-13 métodos para amplificação de ácido nucléico, método de detecção de patógeno e métodos para execução de amplificação de ácido nucléico BRPI0510811B1 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122016013290-0A BR122016013290B1 (pt) 2004-05-13 2005-05-13 dispositivo para amplificação de moléculas de ácido nucleico, dispositivo microfluídico para aplicação de tensão às moléculas de ácido nucléico e kits para processamento de molécula de ácido nucléico

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US57090704P 2004-05-13 2004-05-13
US61679304P 2004-10-06 2004-10-06
PCT/US2005/016638 WO2006076022A2 (en) 2004-05-13 2005-05-13 Nano-pcr: methods and devices for nucleic acid amplification and detection

Publications (2)

Publication Number Publication Date
BRPI0510811A true BRPI0510811A (pt) 2007-11-06
BRPI0510811B1 BRPI0510811B1 (pt) 2018-12-26

Family

ID=36678035

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Application Number Title Priority Date Filing Date
BRPI0510811A BRPI0510811B1 (pt) 2004-05-13 2005-05-13 métodos para amplificação de ácido nucléico, método de detecção de patógeno e métodos para execução de amplificação de ácido nucléico
BR122016013290-0A BR122016013290B1 (pt) 2004-05-13 2005-05-13 dispositivo para amplificação de moléculas de ácido nucleico, dispositivo microfluídico para aplicação de tensão às moléculas de ácido nucléico e kits para processamento de molécula de ácido nucléico

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BR122016013290-0A BR122016013290B1 (pt) 2004-05-13 2005-05-13 dispositivo para amplificação de moléculas de ácido nucleico, dispositivo microfluídico para aplicação de tensão às moléculas de ácido nucléico e kits para processamento de molécula de ácido nucléico

Country Status (11)

Country Link
US (3) US7494791B2 (pt)
EP (2) EP1751314B1 (pt)
JP (1) JP5007440B2 (pt)
KR (1) KR101506727B1 (pt)
AP (1) AP2773A (pt)
AU (2) AU2005324505B2 (pt)
BR (2) BRPI0510811B1 (pt)
CA (2) CA3152535C (pt)
IL (2) IL179006A (pt)
NZ (2) NZ551996A (pt)
WO (1) WO2006076022A2 (pt)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0510811B1 (pt) 2004-05-13 2018-12-26 Goel Anita métodos para amplificação de ácido nucléico, método de detecção de patógeno e métodos para execução de amplificação de ácido nucléico
US20060105433A1 (en) * 2004-11-18 2006-05-18 Bickmore William D Jr Rapid thermocycler
US7315376B2 (en) * 2005-01-07 2008-01-01 Advanced Molecular Systems, Llc Fluorescence detection system
US20060188891A1 (en) * 2005-02-23 2006-08-24 Bickmore William D Jr Methods and apparatus for controlling DNA amplification
DK1948816T3 (da) 2005-10-24 2012-04-02 Univ Johns Hopkins Forbedrede fremgangsmåder til beaming
JP2009534033A (ja) 2006-04-21 2009-09-24 ナノバイオシン，インコーポレイテッド 薬物発見のための単一分子プラットホーム：抗癌剤および抗ウイルス剤の発見を含む薬物発見のための方法および装置
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7312029B1 (en) 2006-06-30 2007-12-25 Searete Llc Method of combing an elongated molecule
US7745129B1 (en) * 2006-07-12 2010-06-29 Kenneth David Schatz Methods for sequencing of a necleic acid
WO2008015396A2 (en) * 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
JP5511669B2 (ja) 2007-10-02 2014-06-04 セラノス， インコーポレイテッド モジュール式ポイントオブケアデバイスおよびその使用
GB2469424B (en) * 2008-02-19 2013-04-17 Intelligent Bio Systems Inc Non-emulsion methods and masked biomolecules
US8741815B2 (en) 2008-02-19 2014-06-03 Intelligent Bio Systems, Inc. Methods and devices for amplification of nucleic acid
JP2010207133A (ja) * 2009-03-10 2010-09-24 Sony Corp 細胞分離方法
EP2437887B1 (en) * 2009-06-04 2016-05-11 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
EP2507220B1 (en) 2009-12-04 2016-10-05 Biotium Inc. Heterocycle-substituted xanthene dyes
CN103210079B (zh) 2010-08-02 2015-07-22 B·L·韦特 用于聚合酶链式反应的可增压筒
MX2013004184A (es) 2010-10-15 2013-07-29 Lockheed Corp Diseño optico microfluidico.
CN106290159A (zh) 2011-01-21 2017-01-04 提拉诺斯公司 样品使用最大化的系统和方法
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
EP2744916A4 (en) 2011-07-13 2015-06-17 Primeradx Inc MULTIMODAL METHODS FOR SIMULTANEOUS DETECTION AND QUANTIFICATION OF MULTIPLE NUCLEIC ACIDS IN A SAMPLE
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
US9044729B2 (en) 2012-07-27 2015-06-02 International Park Of Creativity Methods and devices for electromagnetic amplification of nucleic acids
JP6063693B2 (ja) * 2012-10-03 2017-01-18 株式会社日立ハイテクノロジーズ 分析装置及び分析方法
KR101437261B1 (ko) * 2013-01-17 2014-11-03 한국과학기술원 회전 ｐｃｒ 공정용 시료 처리 칩, 이를 구비하는 회전 ｐｃｒ 장치 및 이를 이용한 회전 ｐｃｒ 방법
US10401373B1 (en) 2013-02-18 2019-09-03 Theranos Ip Company, Llc Systems and methods for analyte testing and laboratory oversight
US11008628B1 (en) 2013-02-18 2021-05-18 Labrador Diagnostics Llc Systems and methods for analyte testing and laboratory oversight
KR102529007B1 (ko) 2013-03-15 2023-05-03 나노바이오심 인크. 핵산들 및 단백질들의 모바일 디바이스 분석을 위한 시스템들 및 방법들
US10933417B2 (en) * 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
US10422806B1 (en) 2013-07-25 2019-09-24 Theranos Ip Company, Llc Methods for improving assays of biological samples
WO2015017604A1 (en) * 2013-08-02 2015-02-05 International Park Of Creativity Methods and devices for electromagnetic ligation of nucleic acids and electromagnetic transformation of cells
CN111007236B (zh) 2013-09-06 2022-01-14 赛拉诺斯知识产权有限责任公司 用于容纳试剂的筒匣、系统、套件和拭子容器
WO2015035260A1 (en) 2013-09-06 2015-03-12 Theranos, Inc. Systems and methods for detecting infectious diseases
EP3077545B1 (en) 2013-12-05 2020-09-16 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
WO2015085275A2 (en) 2013-12-05 2015-06-11 Centrillion Technology Holdings Corporation Fabrication of patterned arrays
CN105940024B (zh) 2013-12-05 2019-03-15 生捷科技控股公司 修饰的表面
US10260111B1 (en) 2014-01-20 2019-04-16 Brett Eric Etchebarne Method of detecting sepsis-related microorganisms and detecting antibiotic-resistant sepsis-related microorganisms in a fluid sample
US11360107B1 (en) 2014-02-25 2022-06-14 Labrador Diagnostics Llc Systems and methods for sample handling
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US10533216B2 (en) 2014-05-23 2020-01-14 Centrillion Technology Holdings Corporation Oligonucleotide probe inversion process for in situ synthesized probe arrays
WO2016004171A1 (en) 2014-07-03 2016-01-07 Centrillion Technology Holdings Corporation Device for storage and dispensing of reagents
WO2016153000A1 (ja) * 2015-03-24 2016-09-29 国立大学法人東京大学 流体デバイス、システム及び方法
EP3280424B1 (en) 2015-04-06 2020-10-14 Centrillion Technology Holdings Corporation Methods for phrasing epigenetic modifications of genomes
WO2016182984A1 (en) * 2015-05-08 2016-11-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
WO2017004502A1 (en) 2015-07-02 2017-01-05 Centrillion Technology Holdings Corporation Systems and methods to dispense and mix reagents
WO2017027779A1 (en) 2015-08-13 2017-02-16 Centrillion Technology Holdings Corporation Library construction using y-adapters and vanishing restriction sites
CN106434873B (zh) 2015-08-13 2021-08-27 生捷科技控股公司 使核酸分子同步化的方法
US10695735B2 (en) 2015-08-18 2020-06-30 Centrillion Technology Holdings Corporation Probe inversion process for in situ synthesized probe arrays
CN105139824B (zh) * 2015-10-16 2018-02-06 重庆京东方光电科技有限公司 栅极驱动器及其配置系统和调节配置方法
CN107841575B (zh) * 2017-11-06 2021-04-23 青岛农业大学 一种区分四种血清型禽腺病毒i群的纳米多重pcr方法
EP3789117A1 (en) * 2019-09-09 2021-03-10 F. Hoffmann-La Roche AG Smartphone pcr device
TR202008937A2 (tr) * 2020-06-09 2020-07-21 Gigabiomol Biyoteknoloji Sanayi Ve Ticaret Ltd Sirketi Elastik Polimer ile Mikroorganizma Konsantrasyon Yöntemi
CN111763718A (zh) * 2020-07-09 2020-10-13 广东省农业科学院动物卫生研究所 一种检测猪链球菌感染的纳米pcr方法

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
DE4027616A1 (de) * 1990-08-31 1992-03-05 Boehringer Mannheim Gmbh Verfahren zur bestimmung von polymerase-aktivitaet
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5824477A (en) 1990-09-12 1998-10-20 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US6197508B1 (en) 1990-09-12 2001-03-06 Affymetrix, Inc. Electrochemical denaturation and annealing of nucleic acid
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
DE69409646T2 (de) 1993-06-09 1998-08-06 Gamera Bioscience Corp Magnetische zyklusreaktionen
ATE175723T1 (de) 1994-03-15 1999-01-15 Scient Generics Ltd Elektrochemische denatusierung von doppelsträngiger nukleinsäure
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US6613560B1 (en) 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
US5683875A (en) * 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
ATE295427T1 (de) * 1996-06-04 2005-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9614544D0 (en) 1996-07-11 1996-09-04 Scient Generics Ltd Denaturation of double-stranded nucleic acid
GB9706654D0 (en) 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
US6391622B1 (en) * 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
CA2256563A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
US6055106A (en) 1998-02-03 2000-04-25 Arch Development Corporation Apparatus for applying optical gradient forces
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6929030B2 (en) 1999-06-28 2005-08-16 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
DE60031540T2 (de) 1999-06-28 2007-05-16 California Institute Of Technology, Pasadena Mikromechanische pump- und ventilsysteme
JP2003507026A (ja) * 1999-08-13 2003-02-25 ユー．エス．ジェノミクス，インコーポレーテッド ポリマーを引き伸ばす方法および装置
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6699713B2 (en) 2000-01-04 2004-03-02 The Regents Of The University Of California Polymerase chain reaction system
US20030045005A1 (en) * 2000-10-17 2003-03-06 Michael Seul Light-controlled electrokinetic assembly of particles near surfaces
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn AMPLIFICATION OF NUCLEIC ACID USING MICROFLUIDIC DEVICES
US6793752B2 (en) * 2001-09-21 2004-09-21 The Goodyear Tire & Rubber Company Precision longitudinal registration of tire building drum to automated tire building system work station
CA2474993A1 (en) 2002-02-21 2003-08-28 Tokyo Gas Company Limited Diagnostic reagent for evalulation of pharmacological effect of medicine, and method and reagent for screening pharmaceutical agents comprising enzyme, enzyme inhibitor or receptor ligand and/or prodrugs thereof for one having high medicative efficacy and/or small side effect
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
BRPI0510811B1 (pt) 2004-05-13 2018-12-26 Goel Anita métodos para amplificação de ácido nucléico, método de detecção de patógeno e métodos para execução de amplificação de ácido nucléico
JP2009534033A (ja) 2006-04-21 2009-09-24 ナノバイオシン，インコーポレイテッド 薬物発見のための単一分子プラットホーム：抗癌剤および抗ウイルス剤の発見を含む薬物発見のための方法および装置

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